CN106492282A - A kind of material for filling allogeneic decalcification bone for nose shaping and preparation method thereof - Google Patents
A kind of material for filling allogeneic decalcification bone for nose shaping and preparation method thereof Download PDFInfo
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- CN106492282A CN106492282A CN201611122678.2A CN201611122678A CN106492282A CN 106492282 A CN106492282 A CN 106492282A CN 201611122678 A CN201611122678 A CN 201611122678A CN 106492282 A CN106492282 A CN 106492282A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3612—Cartilage, synovial fluid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/18—Internal ear or nose parts, e.g. ear-drums
- A61F2/186—Nose parts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
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Abstract
The present invention relates to a kind of material for filling allogeneic decalcification bone for nose shaping and preparation method thereof, wherein, the structure includes that the nasal bone matrix being made by allogeneic decalcification bone, the nasal bone matrix include that nose, nose pillar, the bridge of the nose, the bridge of the nose connect nose pillar by nose；There is between the bridge of the nose and nose the changeover portion for narrowing, the width of the changeover portion to be less than the width of the bridge of the nose；The bottom of the bridge of the nose is made up of two shape that is in the shape of the letter V bridge of the nose sections arranged in a crossed manner, and the nasal bone of human body is located between two bridge of the nose sections, to ensure that prosthese will not displacement and abrasion.Beneficial effect is：By the design of bridge of the nose section so that prosthese is in close contact with nasal bone, will not displacement or abrasion；Body fluid can be allowed to flow into hole by the hole on the bridge of the nose is made tissue grow into hole, allows nose to be connected with nose prosthesiss, prevent nose prosthesiss from moving, come off, festering, and prevents the pain of postoperative patient, this method to be applied to consubstantiality, allograph bone process.
Description
Technical field
The present invention relates to shaping technique field, fills allogeneic decalcification bone more particularly, to a kind of for nose shaping
Material and preparation method thereof.
Background technology
Nasal plastic (face-lifting) operation be by being implanted into suitable material changing height and the form of nose, be allowed to face other
Position is mutually coordinated, and is a kind of modal operation of field of orthopedic surgery.
Medically, nasal plastic material has many kinds, such as medical grade silicon rubber, autologous cartilage, animal tendon or ligament, varicosity
(politef), hyaluronic acid etc..The preparation method of bridge of the nose prosthese is also more, wherein, autologous cartilage due to originating itself, so
Be not in rejection phenomenon, also not sensitization, but moulding relatively difficult in manufacturing process.With animal tendon, ligament as raw material, because
Which constitutes and structure is similar to tissue, good biocompatibility, is implanted into and does not cause immune rejection, and inducible tissue is again
Raw, but need to process through a series of biochemical technologies, manufacturing process is more complicated.Firm silicone rubber hump nose material because nontoxic,
Histocompatibility is relatively stable, is main medical prosthesis hump nose material, is easy to moulding in manufacturing process, but silicone rubber implant
It is susceptible to shift and wear and tear after implanting.
Therefore, have very much must for a kind of appearance of the material and preparation method thereof for filling allogeneic decalcification bone for nose shaping
Want, its manufacturing process is convenient, Stability Analysis of Structures is not susceptible to shift and weares and teares.
Content of the invention
The present invention provides a kind of material for filling allogeneic decalcification bone for nose shaping and preparation method thereof, to solve
In prior art, manufacturing process is more complicated, is susceptible to the problems such as displacement and abrasion.
Technical problem solved by the invention employs the following technical solutions to realize：
A kind of material for filling allogeneic decalcification bone for nose shaping, including being made by allogeneic decalcification bone
Nasal bone matrix, the nasal bone matrix includes nose, nose pillar, the bridge of the nose, and the bridge of the nose connects nose pillar by nose；The nose
There is between beam and nose the changeover portion for narrowing, the width of the changeover portion to be less than the width of the bridge of the nose；
The bottom of the bridge of the nose is made up of two shape that is in the shape of the letter V bridge of the nose sections arranged in a crossed manner, and the nasal bone of human body is located at two
Between the individual bridge of the nose section, to ensure that prosthese will not displacement and abrasion.
Used as preferred technical scheme, the surface of the bridge of the nose is densely covered with numerous holes, by described hole so that prosthese
There is elasticity, can stretch, can be very good nose of fitting, hole can allow body fluid to flow into hole and grow tissue, allow
Nose is connected with nose prosthesiss, is prevented nose prosthesiss from moving, come off, festering, is prevented the pain of postoperative patient.
Used as preferred technical scheme, described hole is through hole or non through hole.
Used as preferred technical scheme, described hole is non through hole, and the bottom of described hole is plane or arc surface.Described
Hole can be typed it out with laser, and laser equipment punched hole, its hole periphery be mellow and full, and bottom is one and smooths, impulse- free robustness.
As preferred technical scheme, the surface for being arranged in the bridge of the nose of described hole rule, described hole and hole
Between spacing identical, overall very attractive in appearance.
Used as preferred technical scheme, the bore dia of described hole is 0.02mm-0.1mm；Hole density is 3000-200000
Individual/cm2.
A kind of preparation method of the material for filling allogeneic decalcification bone for nose shaping, comprises the following steps：
A bone) is taken, allogeneic cartilage is obtained in an aseptic environment；
B) process, the allogeneic cartilage for obtaining is carried out defat, decalcification, deproteinization and sterilization treatment successively；
C) chisel and carve, the allogeneic cartilage after above-mentioned process chiseled and carved into the shape for needing, you can；
Used as preferred technical scheme, the allogeneic cartilage is non-autologous rib, non-Auricular cartilage.
Used as preferred technical scheme, the concrete processing procedure in step B includes：
(1) allogeneic cartilage is immersed 6-24 hours in chloroform and methanol mixed liquid；
(2) allogeneic cartilage after above-mentioned steps (1) process is added in hydrochloric acid solution, persistently stirring 8-12 is little
When；
(3) allogeneic cartilage after above-mentioned steps (2) process is immersed TritonX-100 and ammonia mixed solution
Middle effect 2-72 hours；
(4) by the allogeneic cartilage lyophilization after above-mentioned steps (3) process, sterilization treatment；
Wherein：The volume ratio of chloroform and methanol in the chloroform and methyl alcohol mixed liquor is 1：1；
The concentration of the hydrochloric acid solution is 0.5-0.6mol/L；
The volumn concentration of the TritonX-100 in the TritonX-100 and ammonia mixed solution is 0.1%-
2%, the weight/mass percentage composition of ammonia is 0.3%；
The lyophilization refers to freeze-dried in the freeze dryer that the allogeneic cartilage is put into -72 DEG C～-80 DEG C
24h～48h；
The sterilization treatment is referred to and will be put into ethylene oxide sterilizing after the allogeneic cartilage plastic bag packaging seal
Sterilizing in device, in 480mg/L～520mg/L, sterilising temp is controlled at 38 DEG C～42 DEG C, is gone out for the mass concentration control of oxirane
Bacterium humidity is 45%～55%, and the sterilization functions time is 3h～4h, and sterilization intensity is in 1500mg/h L～2000mg/h L.
Used as preferred technical scheme, the process of chiseling and carving includes beating the allogeneic cartilage by laser equipment
Hole.
Used as preferred technical scheme, the bore dia that laser equipment punches to the allogeneic cartilage is 0.02mm-
0.1mm；Hole density is 3000-200000/cm2.
The invention has the advantages that：By the design of bridge of the nose section so that prosthese is in close contact with nasal bone, Bu Huiwei
Move or wear and tear；Body fluid can be allowed to flow into hole by the hole on the bridge of the nose makes tissue grow into hole, allows nose and nose
Prosthese is connected, and prevents nose prosthesiss from moving, come off, festering, prevents the pain of postoperative patient.
Description of the drawings
In order to be illustrated more clearly that embodiment of the present invention or technical scheme of the prior art, below will be to embodiment
Or accompanying drawing to be used is briefly described needed for description of the prior art, it should be apparent that, drawings in the following description are only
It is some embodiments of the present invention, for those of ordinary skill in the art, in the premise for not paying creative labor
Under, can be with according to these other accompanying drawings of accompanying drawings acquisition.
Fig. 1 is the present invention：A kind of material schematic diagram for filling allogeneic decalcification bone for nose shaping；
Fig. 2 is the present invention：A kind of top view of the material for filling allogeneic decalcification bone for nose shaping；
Fig. 3 is the present invention：A kind of flow process of the preparation method of the material for filling allogeneic decalcification bone for nose shaping
Schematic diagram.
Specific embodiment
In order that technological means, creation characteristic, reached purpose and effect that the present invention is realized are easy to understand, tie below
Conjunction is specifically illustrating, and the present invention is expanded on further.
With reference to shown in Fig. 1-2, a kind of material for filling allogeneic decalcification bone for nose shaping, including by allogeneic
The nasal bone matrix 100 that decalcification bone is made, nasal bone matrix 100 include that nose 1, nose pillar 2, the bridge of the nose 3, the bridge of the nose 3 pass through nose 1
Connection nose pillar 2；The width between the bridge of the nose 3 and nose 1 with the changeover portion 4 for narrowing, the changeover portion 4 is less than the bridge of the nose 3
Width, changeover portion 4 can cause nose more moulding；The bottom of the bridge of the nose 3 is by two shapes that are in the shape of the letter V, 12 groups of bridge of the nose section arranged in a crossed manner
Into, and the nasal bone of human body is between two bridge of the nose sections 12, to ensure that prosthese will not displacement and abrasion.
The surface of the bridge of the nose 3 of the present invention is densely covered with numerous holes 11, causes prosthese that there is elasticity, Ke Yila by hole 11
Stretch, can be very good nose of fitting, hole 11 can allow body fluid to flow into hole 11 and grow tissue, make nose false with nose
Body is connected, and prevents nose prosthesiss from moving, come off, festering, prevents the pain of postoperative patient.Preferably, hole 11 is through hole
Or non through hole.
The hole 11 of the present invention is non through hole, and the bottom of hole 11 is plane or arc surface.Hole 11 can be beaten with laser
Out, laser equipment punched hole, its hole periphery are mellow and full, and bottom is one and smooths, impulse- free robustness.
The surface for being arranged in the bridge of the nose 3 or bridge of the nose section 12 of 11 rule of hole of the present invention, between hole 11 and hole 11
Spacing is identical, overall very attractive in appearance.The bore dia of hole 11 is 0.02mm-0.1mm；Hole density is 3000-200000/cm2.
With reference to shown in Fig. 3, the invention allows for a kind of material for filling allogeneic decalcification bone for nose shaping
Preparation method, comprises the following steps：
A bone) is taken, allogeneic cartilage is obtained in an aseptic environment, wherein, the allogeneic cartilage is non-autologous rib
Bone, non-Auricular cartilage.
B) process, the allogeneic cartilage for obtaining is carried out defat, decalcification, deproteinization and sterilization treatment successively；
C) chisel and carve, the allogeneic cartilage after above-mentioned process chiseled and carved into the shape for needing, you can.
Concrete processing procedure in step B of the present invention includes：
(1) allogeneic cartilage is immersed 6-24 hours in chloroform and methanol mixed liquid；Wherein：The chloroform and
The volume ratio of chloroform and methanol in methyl alcohol mixed liquor is 1：1；
(2) allogeneic cartilage after above-mentioned steps (1) process is added in hydrochloric acid solution, persistently stirring 8-12 is little
When；The concentration of the hydrochloric acid solution is 0.5-0.6mol/L；
(3) allogeneic cartilage after above-mentioned steps (2) process is immersed TritonX-100 and ammonia mixed solution
Middle effect 2-72 hours；The volumn concentration of the TritonX-100 in the TritonX-100 and ammonia mixed solution is
0.1%-2%, the weight/mass percentage composition of ammonia is 0.3%；
(4) by the allogeneic cartilage lyophilization after above-mentioned steps (3) process, sterilization treatment；Wherein, described cold
Lyophilizing is dry to refer to freeze-dried 24h～48h in the freeze dryer that the allogeneic cartilage is put into -72 DEG C～-80 DEG C；Described go out
Bacterium process is referred to and will be put into sterilizing, epoxy second in ethylene oxide sterilizer after the allogeneic cartilage plastic bag packaging seal
In 480mg/L～520mg/L, sterilising temp is controlled at 38 DEG C～42 DEG C for the mass concentration control of alkane, and sterilizing humidity is 45%～
55%, the sterilization functions time is 3h～4h, and sterilization intensity is in 1500mg/h L～2000mg/h L.
The process of chiseling and carving in step C of the present invention includes punching the allogeneic cartilage by laser equipment,
Wherein, bore dia is 0.02mm-0.1mm；Hole density is 3000-200000/cm2.
Embodiment 1
1st, non-autologous rib cartilage is obtained in an aseptic environment；It is preferred that the 11st section or the 12nd section rib is taken, with distillation
Water is cleaned up and is soaked；
2nd, immersing the allogeneic cartilage in chloroform and methanol mixed liquid carries out defat in 24 hours；Wherein：Described
The volume ratio of chloroform and methanol in chloroform and methyl alcohol mixed liquor is 1：1, cleaned with distilled water after taking-up；
3rd, by defat after allogeneic cartilage immersion concentration for the hydrochloric acid solution of 0.5mol/L in, and persistently stirring is 12 little
Shi Jinhang decalcifications, are cleaned with distilled water after taking-up；
4th, by decalcification after allogeneic cartilage immersion TritonX-100 and ammonia mixed solution in act on and carrying out for 72 hours
Deproteinization；Wherein, the volumn concentration of the TritonX-100 in the TritonX-100 and ammonia mixed solution is
1.5%, the weight/mass percentage composition of ammonia is 0.3%, is cleaned with distilled water after taking-up；
5th, by deproteinization after allogeneic cartilage be placed in -80 DEG C of freeze dryer after freeze-dried 24h, use plastic bag bag
Sterilizing in ethylene oxide sterilizer is put into after dress sealing, and the mass concentration of oxirane is controlled in 500mg/L, and sterilising temp is 38
DEG C, sterilizing humidity is 45%, and the sterilization functions time is 3h, and sterilization intensity is in 1500mg/h L.
The 6th, allogeneic cartilage after above-mentioned process is chiseled and carved into the shape for needing, you can be implanted into.Of the same race different in order to ensure
Body cartilage is stablized, and by laser equipment to its surface punching, wherein, bore dia is 0.05mm to the allogeneic cartilage；Hole
Density is 10000/cm2.
Embodiment 2
1st, non-Auricular cartilage is obtained in an aseptic environment；Cleaned up with distilled water and soaked；
2nd, immersing the allogeneic cartilage in chloroform and methanol mixed liquid carries out defat in 24 hours；Wherein：Described
The volume ratio of chloroform and methanol in chloroform and methyl alcohol mixed liquor is 1：1, cleaned with distilled water after taking-up；
3rd, by defat after allogeneic cartilage immersion concentration for the hydrochloric acid solution of 0.6mol/L in, and persistently stirring is 12 little
Shi Jinhang decalcifications, are cleaned with distilled water after taking-up；
4th, by decalcification after allogeneic cartilage immersion TritonX-100 and ammonia mixed solution in act on and carrying out for 72 hours
Deproteinization；Wherein, the volumn concentration of the TritonX-100 in the TritonX-100 and ammonia mixed solution is 1%,
The weight/mass percentage composition of ammonia is 0.3%, is cleaned with distilled water after taking-up；
5th, by deproteinization after allogeneic cartilage be placed in -80 DEG C of freeze dryer after freeze-dried 24h, use plastic bag bag
Sterilizing in ethylene oxide sterilizer is put into after dress sealing, and the mass concentration of oxirane is controlled in 500mg/L, and sterilising temp is 40
DEG C, sterilizing humidity is 45%, and the sterilization functions time is 3h, and sterilization intensity is in 2000mg/h L.
The 6th, allogeneic cartilage after above-mentioned process is chiseled and carved into the shape for needing, you can be implanted into.Of the same race different in order to ensure
Body cartilage is stablized, and by laser equipment to its surface punching, wherein, bore dia is 0.05mm to the allogeneic cartilage；Hole
Density is 15000/cm2.
Embodiment 3
1st, non-autologous rib cartilage is obtained in an aseptic environment；It is preferred that the 11st section or the 12nd section rib is taken, with distillation
Water is cleaned up and is soaked；
2nd, immersing the allogeneic cartilage in chloroform and methanol mixed liquid carries out defat in 24 hours；Wherein：Described
The volume ratio of chloroform and methanol in chloroform and methyl alcohol mixed liquor is 1：1, cleaned with distilled water after taking-up；
3rd, by defat after allogeneic cartilage immersion concentration for the hydrochloric acid solution of 0.6mol/L in, and persistently stirring is 12 little
Shi Jinhang decalcifications, are cleaned with distilled water after taking-up；
4th, by decalcification after allogeneic cartilage immersion TritonX-100 and ammonia mixed solution in act on and carrying out for 72 hours
Deproteinization；Wherein, the volumn concentration of the TritonX-100 in the TritonX-100 and ammonia mixed solution is
0.5%, the weight/mass percentage composition of ammonia is 0.3%, is cleaned with distilled water after taking-up；
5th, by deproteinization after allogeneic cartilage be placed in -80 DEG C of freeze dryer after freeze-dried 24h, use plastic bag bag
Sterilizing in ethylene oxide sterilizer is put into after dress sealing, and the mass concentration of oxirane is controlled in 500mg/L, and sterilising temp is 38
DEG C, sterilizing humidity is 45%, and the sterilization functions time is 3h, and sterilization intensity is in 1500mg/h L.
The 6th, allogeneic cartilage after above-mentioned process is chiseled and carved into the shape for needing, you can be implanted into.Of the same race different in order to ensure
Body cartilage is stablized, and by laser equipment to its surface punching, wherein, bore dia is 0.08mm to the allogeneic cartilage；Hole
Density is 8000/cm2.
Comparing result
Nasal bone prosthese is made according to the preparation method of above-described embodiment 1,2,3 respectively, and is implanted in corresponding human body
It is tracked and observes, statistical data is shown in Table lattice 1.
Form 1
Result surface prosthesis implantation effect is good, and without rejection, healing is good.
As the material of the present invention is using allogeneic decalcification cartilage, without rejection, and hole is provided with the surface of cartilage, is led to
Crossing hole can allow body fluid inflow hole to make tissue grow into hole, allow nose to be connected with nose prosthesiss, prevent nose prosthesiss
Move, come off, fester, prevent the pain of postoperative patient.
The ultimate principle and principal character and advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel it should be appreciated that the present invention is not restricted to the described embodiments, simply explanation described in above-described embodiment and description this
The principle of invention, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, these changes
Change and improvement is both fallen within scope of the claimed invention.The claimed scope of the invention by appending claims and its
Equivalent thereof.
Claims (10)
1. a kind of for nose shaping fill allogeneic decalcification bone material, it is characterised in that include by allogeneic decalcification
The nasal bone matrix that bone is made, the nasal bone matrix include that nose, nose pillar, the bridge of the nose, the bridge of the nose connect nose by nose
Pillar；There is between the bridge of the nose and nose the changeover portion for narrowing, the width of the changeover portion to be less than the width of the bridge of the nose；
The bottom of the bridge of the nose is made up of two shape that is in the shape of the letter V bridge of the nose sections arranged in a crossed manner, and the nasal bone of human body is located at two institutes
State between bridge of the nose section.
2. according to claim 1 a kind of for nose shaping fill allogeneic decalcification bone material, it is characterised in that
The surface of the bridge of the nose is densely covered with numerous holes.
3. according to claim 2 a kind of for nose shaping fill allogeneic decalcification bone material, it is characterised in that
Described hole is through hole or non through hole.
4. according to claim 3 a kind of for nose shaping fill allogeneic decalcification bone material, it is characterised in that
Described hole is non through hole, and the bottom of described hole is plane or arc surface.
5. according to claim 2 a kind of for nose shaping fill allogeneic decalcification bone material, it is characterised in that
The surface for being arranged in the bridge of the nose of described hole rule, the spacing between described hole and hole are identical.
6. a kind of for nose shaping fill allogeneic decalcification bone material preparation method, it is characterised in that including following
Step：
A bone) is taken, allogeneic cartilage is obtained in an aseptic environment；
B) process, the allogeneic cartilage for obtaining is carried out defat, decalcification, deproteinization and sterilization treatment successively；
C) chisel and carve, the allogeneic cartilage after above-mentioned process is chiseled and carved into the use as described in claim 1-5 any claim
In the material that allogeneic decalcification bone is filled in nose shaping, you can.
7. according to claim 6 a kind of for nose shaping fill allogeneic decalcification bone material preparation method,
Characterized in that, the allogeneic cartilage is non-autologous rib, non-Auricular cartilage.
8. according to claim 6 a kind of for nose shaping fill allogeneic decalcification bone material preparation method,
Characterized in that, the concrete processing procedure in step B includes：
(1) allogeneic cartilage is immersed 6-24 hours in chloroform and methanol mixed liquid；
(2) allogeneic cartilage after above-mentioned steps (1) process is added in hydrochloric acid solution, persistently stirs 8-12 hours；
(3) allogeneic cartilage after above-mentioned steps (2) process is immersed in TritonX-100 and ammonia mixed solution and is made
Use 2-72 hours；
(4) by the allogeneic cartilage lyophilization after above-mentioned steps (3) process, sterilization treatment；
Wherein：The volume ratio of chloroform and methanol in the chloroform and methyl alcohol mixed liquor is 1：1；
The concentration of the hydrochloric acid solution is 0.5-0.6mol/L；
The volumn concentration of the TritonX-100 in the TritonX-100 and ammonia mixed solution be 0.1%-2%, ammonia
Weight/mass percentage composition be 0.3%；
The lyophilization refer to freeze-dried 24h in the freeze dryer that the allogeneic cartilage is put into -72 DEG C～-80 DEG C～
48h；
The sterilization treatment is referred to and will be put in ethylene oxide sterilizer after the allogeneic cartilage plastic bag packaging seal
Sterilizing, in 480mg/L～520mg/L, sterilising temp is controlled at 38 DEG C～42 DEG C, is sterilized wet for the mass concentration control of oxirane
Spend for 45%～55%, the sterilization functions time is 3h～4h, and sterilization intensity is in 1500mg/h L～2000mg/h L；Or
The sterilization treatment is irradiation sterilization, and the ultraviolet light of irradiation is 265-266nm, and the sterilization functions time is 3h～4h.
9. according to claim 6 a kind of for nose shaping fill allogeneic decalcification bone material preparation method,
Characterized in that, the process of chiseling and carving includes punching the allogeneic cartilage by laser equipment.
10. according to claim 9 a kind of for nose shaping fill allogeneic decalcification bone material preparation method,
Characterized in that, the bore dia that laser equipment punches to the allogeneic cartilage is 0.02mm-0.1mm；Hole density is 3000-
200000/cm2.
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Application publication date: 20170315 |