CN106489720A - Soya bean mutually-acting nucleus male-sterile line NJCMS4A and its selection - Google Patents
Soya bean mutually-acting nucleus male-sterile line NJCMS4A and its selection Download PDFInfo
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- CN106489720A CN106489720A CN201610956231.9A CN201610956231A CN106489720A CN 106489720 A CN106489720 A CN 106489720A CN 201610956231 A CN201610956231 A CN 201610956231A CN 106489720 A CN106489720 A CN 106489720A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Genetics & Genomics (AREA)
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Abstract
The invention discloses soya bean mutually-acting nucleus male-sterile line NJCMS4A and its selection, belong to the breeding technique field of male sterility of crops system；Sterile line NJCMS4A is that using Soybean Native Varieties N23658 as male parent, hybridization obtains F using Soybean Native Varieties N23661 as female parent1For seed；Obtain F1Dai Houyu N23658 are returned；Afterwards with backcross progeny as female parent, N23658 is male parent, carries out continuous backcross, and the economical character for selecting is consistent with male parent, and shows male sterile colony；Which is returned male parent used and is male sterility maintainer line NJCMS4B, and have found restorer south holy 9403, it is achieved that " three are " is supporting.The present invention provides the new i.e. male sterile cytoplasm N23661 in Cytoplasm source for the selection-breeding of soya bean mutually-acting nucleus male-sterile line, has widened the hereditary basiies of soya bean mutually-acting nucleus male-sterile line.
Description
Technical field
A kind of the present invention relates to breeding method of hybrid crop, more particularly to soya bean mutually-acting nucleus male-sterile line
NJCMS4A and its selection, belong to the breeding technique field of male sterility of crops system.
Background technology
Heterotic utilization is the important channel of crops raising per unit area yield.Heterotic utilization makes Oryza sativa L., Semen Maydiss
It is significantly improved Deng staple crops per unit area yield.Utilization of the crop hybrid superiority in agricultural production mainly passes through cenospecies
Popularization is carried out.Cross breeding seed is mainly carried out using male sterility line.Male sterility tie up to have in heterosis utilization important
Effect.
Semen sojae atricolor is important protein and oil crop, and worldwide extensively plants.But, current China Semen sojae atricolor
The 5% or so of the total product in the total output Zhi Zhan world, and per unit area yield is far below Semen sojae atricolor main production country.According to statistics, the heterosis rate of Semen sojae atricolor is
13-20%, even more high, therefore it is one of main target of domestic and international soybean breeder for a long time that soybean heterosis are utilized.
At present, domestic some soya bean mutually-acting nucleus male-sterile lines existing, and have 12 hybrid soybean kinds by authorization,
But still have no the spread of hybrid soybean seeds.Trace it to its cause, main including 3：（1）Existing soya cells matter male
Sterile line outcrossing seed-setting rate is low, causes cross breeding seed inefficient；（2）Part hybrid F1Fertility affected by environment and unstable
Fixed；（3）Existing sterile line is difficult to make influential point set.This 3 aspect reason is probably by Semen sojae atricolor sterile line, particularly not
Hatching cell cytoplasmic inheritance basis is narrow to cause.The Cytoplasm of China's soya bean mutually-acting nucleus male-sterile line is mainly derived from 4 cultivations
Training Semen sojae atricolor, is the cultivation bean from Henan and Hubei Province.As Cytoplasm source is few, significantly limit Semen sojae atricolor sterile line and exist
Utilization on hybrid seeding and hybrid vigor.Therefore, excavate the sterile cytoplasm of new sources and selection-breeding has new sterile cytoplasm
The soya bean mutually-acting nucleus male-sterile line in source is an important content during soybean heterosis are utilized.
Content of the invention
1st, invention technical problem to be solved
It is an object of the invention to solve the problems, such as that Cytoplasm source is few in soybean heterosis utilization, different by selecting
The material in ecotope source, random preparation group merge continuous backcross, find the males different from existing sterile cytoplasm source not
Hatching cell matter, and with this Cytoplasm selection-breeding soya bean mutually-acting nucleus male-sterile line.
2nd, technical scheme
To achieve these goals, the technical solution adopted in the present invention is：
Soya bean mutually-acting nucleus male-sterile line NJCMS4A, sterile line NJCMS4A are made with Soybean Native Varieties N23661
For female parent, using Soybean Native Varieties N23658 as male parent, hybridize to obtain F1For seed；Obtain F1Dai Houyu N23658 are returned；
Afterwards with backcross progeny as female parent, N23658 is male parent, carries out continuous backcross, and the economical character for selecting is consistent with male parent,
And show male sterile colony.
The maternal Soybean Native Varieties N23661 of male sterility line NJCMS4A of the present invention is from the wide of the 6th ecotope
Dong Sheng Dapu Counties, and contain male sterile cytoplasm.
The soybean male sterile cytoplasm N23661 different from existing sterile cytoplasm source are male sterility line NJCMS4A
Maternal through the following steps that filtering out：
(1) random choose covers the wild material of 6 ecotope of Semen sojae atricolor, local varieties；
(2) combination prepared by the material for randomly choosing the identical florescence in selection kind, obtains F1Proceed to be returned after generation；
(3) the method investigation pollen germination rate that is sprouted using sucrose in full-bloom stage, identifies the fertility of offspring；
According to the pollen of F1 generation reciprocal crossess and backcross progeny can degree of educating be pollen germination rate data, filter out with kytoplasm
The soybean male sterile cytoplasm of sterile gene.
The male parent Soybean Native Varieties N23658 of male sterility line NJCMS4A of the present invention from Sichuan Province Xingwen County,
And contain male sterility nucleus.
Maintainer NJCMS4B of soya bean mutually-acting nucleus male-sterile line NJCMS4A of the present invention is Soybean Native Varieties
N23658；Restorer can be south holy 9403.
A kind of method of selection-breeding soya bean mutually-acting nucleus male-sterile line NJCMS4A, concrete operations are：With Soybean Native Varieties
N23661 is female parent as the Cytoplasm donor of sterile line breeding, using Soybean Native Varieties N23658 as the thin of sterile line breeding
Karyon donor is male parent, hybridizes to obtain F1For seed；Obtain F1Dai Houyu N23658 are returned；Afterwards with backcross progeny as female parent,
N23658 is male parent, carries out continuous backcross, consistent with male parent until therefrom selecting economical character, and performance is male sterile not
It is NJCMS4A to educate.
3rd, beneficial effect
(1) the sterile cytoplasm donor of soya bean mutually-acting nucleus male-sterile line NJCMS4A of the invention is Soybean Native Varieties
N23661, source differences are big compared with other sterile lines, can widen the hereditary basiies of soya bean mutually-acting nucleus male-sterile line, be
Hybrid soybean seeds production provides the sterile line with different cytoplasm source.
(2) male parent used by present invention backcrossing is male sterility maintainer line NJCMS4B, and have found restorer south holy 9403,
Achieve " three are " supporting.
Specific embodiment
Below example facilitates a better understanding of the present invention, but does not limit the present invention.
Embodiment 1：
Germplasm materials are selected：Random choose covers 43 parts of wild materials of 6 ecotope of Semen sojae atricolor, 44 parts of local varieties.
Cross combination is prepared：Combination prepared by the material for randomly choosing the identical florescence, obtains F1Proceed to be returned after generation.
Offspring's Fertility identification：In the method investigation pollen germination rate that full-bloom stage is sprouted using sucrose.
Fertility identification is carried out to material to be tested, finds [(N23661 × N23658) F1× N23658] BC that combines1F1Dai Cheng
Existing partial sterility characteristic, pollen germination rate is 24.00-55.03%.
The reciprocal crossess pollen germination rate investigation of above-mentioned cross combination is found：16 plants in Orthogonal Composite N23661 × N23658
F1Pollen germination rate be 50% or so, 10 plants of F are combined in its reciprocal cross1Pollen germination rate be more than 75%.
Proceed continuous backcross to above-mentioned cross combination, pollen germination test finds, BC2F1Dai Zhong, 5 plants of pollen germinations
Rate is 0.13-6.73%；BC3F1Dai Zhong, 55 plants of pollen germination rates are 0%, and 8 plants of pollen germination rates are 0.50-2.10%；BC4F1Generation
In, 41 plants of pollen germination rates are 0%, and 9 plants of pollen germination rates are 0.38-1.49%.
According to the survey result of above pollen germination rate, may infer that maternal N23661 has cytoplasmic sterility gene, with return
The increase of number of times, backcross progeny sterile degree is handed over also to improve therewith；Final backcrossing has obtained the plant that abortion rate is 100%, so as to obtain
A new soya bean mutually-acting nucleus male-sterile line NJCMS4A is obtained, its maintainer NJCMS4B is male parent N23658.
Holy 9403 pollen germination rate in test cross combination NJCMS4A × south is 54.29-79.21%, south sage 9403 should be one extensive
Multiple system.
Cytoplasm donor in the present invention is Soybean Native Varieties N23661, with existing sterile cytoplasm source not phase
With.
The invention is not restricted to above-described embodiment, all are belonged to using the technical scheme that equivalent or equivalence replacement are formed
The scope of protection of present invention.
Claims (7)
1. soya bean mutually-acting nucleus male-sterile line NJCMS4A, it is characterised in that the male sterility line NJCMS4A is with Semen sojae atricolor
Local varieties N23661 are female parent as Cytoplasm donor, are male parent using Soybean Native Varieties N23658 as cell nucleus donor,
Hybridize to obtain F1For seed；Obtain F1Dai Houyu N23658 are returned；Afterwards with backcross progeny as female parent, N23658 is male parent, enters
Row continuous backcross, and the economical character for selecting is consistent with male parent, and show male sterile colony.
2. soya bean mutually-acting nucleus male-sterile line NJCMS4A according to claim 1, it is characterised in that the male is not
Educate be NJCMS4A Cytoplasm donor Soybean Native Varieties N23661 from the Guangdong Province Dapu County of the 6th ecotope, and containing hero
Property sterile cytoplasm.
3. soya bean mutually-acting nucleus male-sterile line NJCMS4A according to claim 1, it is characterised in that the male is not
Educate be NJCMS4A Cytoplasm donor Soybean Native Varieties N23661 through the following steps that filtering out：
(1) random choose covers the wild material of 6 ecotope of Semen sojae atricolor, local varieties；
(2) combination prepared by the material for randomly choosing the identical florescence in selection kind, obtains F1Proceed to be returned after generation；
(3) the method investigation pollen germination rate that is sprouted using sucrose in full-bloom stage, identifies the fertility of offspring；
(4) according to F1For reciprocal crossess and backcross progeny pollen can degree of educating be pollen germination rate data, filter out with cytoplasmic sterility
The soybean male sterile cytoplasm of gene.
4. soya bean mutually-acting nucleus male-sterile line NJCMS4A according to claim 1, it is characterised in that the male is not
Educate be NJCMS4A cell nucleus donor Soybean Native Varieties N23658 from Sichuan Province Xingwen County, and contain male sterility cell
Core.
5. soya bean mutually-acting nucleus male-sterile line NJCMS4A according to claim 1, it is characterised in that the Semen sojae atricolor matter
Maintainer NJCMS4B of core interaction male sterility line NJCMS4A is Soybean Native Varieties N23658.
6. soya bean mutually-acting nucleus male-sterile line NJCMS4A according to claim 1, it is characterised in that the Semen sojae atricolor matter
The restorer of core interaction male sterility line NJCMS4A is south holy 9403.
7. a kind of selection of soya bean mutually-acting nucleus male-sterile line NJCMS4A described in claim 1, it is characterised in that institute
The concrete operations for stating method are：It is female parent using Soybean Native Varieties N23661 as the Cytoplasm donor of sterile line breeding, with big
Bean local varieties N23658 are male parent as the cell nucleus donor of sterile line breeding, hybridize to obtain F1For seed；Obtain F1Dai Houyu
N23658 is returned；Afterwards with backcross progeny as female parent, N23658 is male parent, carries out continuous backcross, until therefrom selecting
Economical character is consistent with male parent, and shows male sterile sterile line NJCMS4A.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610956231.9A CN106489720A (en) | 2016-10-27 | 2016-10-27 | Soya bean mutually-acting nucleus male-sterile line NJCMS4A and its selection |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610956231.9A CN106489720A (en) | 2016-10-27 | 2016-10-27 | Soya bean mutually-acting nucleus male-sterile line NJCMS4A and its selection |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1196877A (en) * | 1998-04-09 | 1998-10-28 | 安徽省农业科学院作物研究所 | Selectively breeding method of soya bean mutually-acting nucleus male-sterile line |
| CN101663994A (en) * | 2009-09-24 | 2010-03-10 | 安徽省农业科学院作物研究所 | Seed production method of hybridized summer-sown soybean |
-
2016
- 2016-10-27 CN CN201610956231.9A patent/CN106489720A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1196877A (en) * | 1998-04-09 | 1998-10-28 | 安徽省农业科学院作物研究所 | Selectively breeding method of soya bean mutually-acting nucleus male-sterile line |
| CN101663994A (en) * | 2009-09-24 | 2010-03-10 | 安徽省农业科学院作物研究所 | Seed production method of hybridized summer-sown soybean |
Non-Patent Citations (2)
| Title |
|---|
| 李曙光: "大豆新质核互作雄性不育系的选育及NJCMS3A雄性育性基因的定位", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 赵团结等: "大豆不育细胞质资源的发掘与鉴定", 《作物学报》 * |
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