CN106483070A - It is applied to flow cell and its method for work of liquid on-line detection - Google Patents
It is applied to flow cell and its method for work of liquid on-line detection Download PDFInfo
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- CN106483070A CN106483070A CN201611180253.7A CN201611180253A CN106483070A CN 106483070 A CN106483070 A CN 106483070A CN 201611180253 A CN201611180253 A CN 201611180253A CN 106483070 A CN106483070 A CN 106483070A
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- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000007788 liquid Substances 0.000 title claims abstract description 24
- 238000001514 detection method Methods 0.000 title claims description 50
- 238000007789 sealing Methods 0.000 claims abstract description 44
- 239000013307 optical fiber Substances 0.000 claims abstract description 14
- 239000012528 membrane Substances 0.000 claims abstract description 13
- 238000010521 absorption reaction Methods 0.000 claims abstract description 9
- 238000012360 testing method Methods 0.000 claims abstract description 7
- 239000000126 substance Substances 0.000 claims abstract description 4
- 239000012530 fluid Substances 0.000 claims description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000003480 eluent Substances 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 8
- 239000013536 elastomeric material Substances 0.000 claims description 3
- 238000012986 modification Methods 0.000 claims description 3
- 230000004048 modification Effects 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 241000208340 Araliaceae Species 0.000 claims 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims 1
- 235000003140 Panax quinquefolius Nutrition 0.000 claims 1
- 235000008434 ginseng Nutrition 0.000 claims 1
- 241000195493 Cryptophyta Species 0.000 description 4
- 238000007689 inspection Methods 0.000 description 4
- 239000003053 toxin Substances 0.000 description 4
- 231100000765 toxin Toxicity 0.000 description 4
- 108700012359 toxins Proteins 0.000 description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical group [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 229920005479 Lucite® Polymers 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000003319 supportive effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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Abstract
The present invention relates to a kind of be applied to flow cell and its method for work that liquid on-line detects, described flow cell includes：Briquetting, described briquetting is provided with first through hole, the second through hole and third through-hole, and the bottom surface of described briquetting is provided with the first array groove, and described first array groove is embedded with first group of magnetic part；Sealing member, described sealing member is provided with fourth hole, fifth hole；Support member, the groove that described support member upper surface is provided with the second array groove and places elastic component, the second array groove is embedded with second group of magnetic part, and described second group of magnetic part and first group of magnetic part are oppositely arranged and attract each other；Chip of light waveguide, described chip of light waveguide is located between described elastic component and sealing member, and chip of light waveguide is provided with sensing area and optical fiber interface, and described sensing area is fixed with the modified membrane of absorption test substance.The present invention has the advantages that small volume, good airproof performance, easy accessibility, stability are high.
Description
Technical field
The present invention relates to liquid on-line detection field, carry out liquid on-line detection particularly to one kind using chip of light waveguide
Flow cell and its method of work.
Background technology
In liquid detecting technology, online circulation microchip detection technique is little because of sample volume needed for it, detection sensitivity
Height, is widely used the advantages of detection speed is fast.The core of online circulation microchip detection is the assembling of flow cell, mesh
Front assembling mode is as follows：
1st, design top board, lower platen, by way of bolt is tightened, chip are fixed between top board and lower platen,
But there is bolt and tighten complex operation, and degree of tightening can because people, dimension and different, uncertain factor is excessive, thus leading to device
Stability is poor, and testing result concordance is poor.
2nd, chip is fixed on by way of guide rod and spring compression by design cover plate, base plate, alignment pin and hold down gag
Between cover plate and base plate, but there is the problems such as assembly parts volume is bigger than normal, and base plate chips flatness requirement cannot meet.
Content of the invention
In order to solve the deficiency in above-mentioned prior art, the invention provides a kind of small volume, easy accessibility, sealing
Property good, interference less, the high flow cell of stability.
The purpose of the present invention is achieved through the following technical solutions：
A kind of flow cell being applied to liquid on-line detection, described flow cell includes：
Briquetting, described briquetting is provided with first through hole, the second through hole and third through-hole, and the bottom surface of briquetting is provided with the first array
Groove, described first array groove is embedded with first group of magnetic part；
Sealing member, described sealing member is provided with fourth hole, fifth hole, and described first through hole and the second through hole are described close
Sealing projection in the plane fall in described fourth hole, described first through hole, the second through hole and fourth hole constitute institute
State the fluid passage of flow cell；Described sealing member is elastomeric material, positioned between described briquetting and support member；
Support member, described support member upper surface is provided with the second array groove, and described second array groove is embedded with second group
Magnetic part, described second group of magnetic part and first group of magnetic part are oppositely arranged and attract each other so that described briquetting and described sealing
Part, chip of light waveguide form the fluid passage of sealing；Described support member upper surface is additionally provided with the groove placing elastic component, described bullet
Property part upper surface be higher than described support member upper surface；
Chip of light waveguide, described chip of light waveguide is provided with sensing area and optical fiber interface, and sensing area is described
Sealing member projection in the plane fall in described fourth hole, described sensing area is fixed with absorption test substance
Modified membrane；Described third through-hole, fifth hole up/down perforation, pencil optical fiber connects described optical fiber interface, and passes through up and down from described
Stretch out in logical passage；Described chip of light waveguide is located between described elastic component and sealing member, and it is in described support member upper table
The distance of the view field edge in face to described second group of magnetic part is more than zero.
According to above-mentioned flow cell it is preferable that the side of described support member is provided with two installing holes, for inserting heating unit
Part and temperature element.
According to above-mentioned flow cell, alternatively, the bottom surface of described support member is provided with temperature chamber, and described temperature chamber is embedded with
Water influent pipeline, fluid passage described in the outlet of described water influent pipeline.
According to above-mentioned flow cell, alternatively, described temperature chamber passes through cover plate for sealing.
According to above-mentioned flow cell it is preferable that described first group of magnetic part includes the magnetic part of two kinds of polarity, described second
Organize the opposite polarity of the magnetic part in magnetic part and the relative magnetic part in position in first group of magnetic part；
If magnetic part number is 3-5 in first group of magnetic part, the polarity of at least two adjacent magnetic parts is identical；
If in first group of magnetic part magnetic part number be N, N >=6 and be even number, then the pole of at least N/2 adjacent magnetic part
Property is identical；
If in first group of magnetic part magnetic part number be M, M >=7 and for odd number when, then at least (M-1)/2 adjacent magnetic
The polarity of part is identical.
According to above-mentioned flow cell it is preferable that described sealing member arrives at the view field edge of described support member upper surface
The distance of described second group of magnetic part is more than zero so that first group of magnetic part and second group of magnetic part directly contact attract, and increases
Sealing between briquetting and support member.
According to above-mentioned flow cell, alternatively, at least two in described first group of magnetic part and/or second group of magnetic part
Magnetic part is with regard to the central axis centrosymmetry of described flow cell.
According to above-mentioned flow cell, alternatively, described first group of magnetic part and second group of magnetic part are along respective place face
Circumferential direction is uniformly distributed.
According to above-mentioned flow cell it is preferable that described chip of light waveguide further includes：Reference region, described reference region is not
Through fluid passage；
Light source injects described flow cell through pencil optical fiber, is divided into two-way, and a road entrance reference region forms reference light paths；One tunnel
Entering signal sensing unit forms detection light path.
According to above-mentioned flow cell, alternatively, described elastic component is O-ring.
According to above-mentioned flow cell it is preferable that described briquetting is made up of lucite.
According to above-mentioned flow cell it is preferable that described support member is aluminum material.
The present invention also provides the method for work of the flow cell that one such application detects, described method of work in liquid on-line
Including：
(A1) sample enters fluid passage, and component to be measured is adsorbed by the modified membrane in sensing area；
(A2) buffer enters fluid passage and displaces sample；
(A3) light source injects described flow cell, and a road enters reference light paths, and a road enters detection light path；
(A4) reference light paths output reference signal I1, detection light path output detection signal I simultaneously2, according to I2/I1Ratio with
The relation of concentration of component to be measured obtains the content of component to be measured in sample.
According to above-mentioned method of work it is preferable that described method of work further includes：
(B1) eluent rinses described fluid passage, and eluting is modified the component to be measured of film absorption；
(B2) buffer flows through described fluid passage, displaces eluent.
According to above-mentioned method of work, alternatively, sensing area includes at least two subregions, described at least two subregions
On be fixed with the modified membrane adsorbing different components to be measured, detection light path exports the detection signal of each subregion, obtains in sample not
Content with component to be measured.
The present invention also provides the method for work of the flow cell that one such application detects, described method of work in liquid on-line
Including：
(C1) buffer enters fluid passage, and light source injects described flow cell, and a road enters reference light paths, and a road enters inspection
Light-metering road；
(C2) using the output signal of reference light paths as temperature indication signal, after temperature indication signal is stable, detection light
Road exports control signal I0；
(C3) sample enters described fluid passage, and component to be measured is adsorbed by described modified membrane；
(C4) buffer enters fluid passage and displaces sample, after temperature indication signal is stable, detection light path output inspection
Survey signal I2；
(C5) according to I2/I0What ratio and concentration of component to be measured closed is the content obtaining component to be measured in sample.
Compared with prior art, the device have the advantages that being：
1st, the flow cell of the present invention, using the setting up and down relative magnetic part in sealing member, is attracted each other by magnetic part
Mode briquetting and support member are sealed, easy accessibility, good airproof performance.
2nd, the present invention arranges heating element heater and temperature element it is ensured that temperature constant in detection process, exclusion on support member
Ambient temperature affects, and improves detection stability.
3rd, the through hole on the through hole and sealing member that the present invention opens up on briquetting constitutes microchannel for liquid communication, sample
Consumption is low, be simple to manufacture, device volume is little.
4th, the present invention adopts chip of light waveguide to detect, in the environment of buffer exists, detection is it is ensured that matrix refractive index one
Cause, exclude matrix interference；Pass through to export reference signal and detection signal simultaneously, improve the accuracy of testing result.
Brief description
Referring to the drawings, the disclosure will be easier to understand.Skilled addressee readily understands that be：This
A little accompanying drawings are used only for illustrating technical scheme, and are not intended to protection scope of the present invention is construed as limiting.
In figure：
Fig. 1 is the stereochemical structure separation graph of the flow cell of the embodiment of the present invention 1；
Fig. 2 be the embodiment of the present invention 1 flow cell in the upper bottom surface of briquetting and the structure diagram of bottom surface；
Fig. 3 be the embodiment of the present invention 1 flow cell in the upper bottom surface of sealing member and the structure diagram of bottom surface；
Fig. 4 be the embodiment of the present invention 1 flow cell in the upper bottom surface of support member and the structure diagram of bottom surface.
Specific embodiment
Fig. 1-4 and following description describe the optional embodiment of the present invention to instruct how those skilled in the art implement
With the reproduction present invention.In order to instruct technical solution of the present invention, some conventional aspects are simplified or have eliminated.Those skilled in the art
It should be appreciated that will within the scope of the invention from the modification of these embodiments or replacement.Those skilled in the art should understand that
Following characteristics can combine in every way to form multiple modifications of the present invention.Thus, the invention is not limited in following can
Embodiment, and only limited by claim and their equivalent.
Embodiment 1
Fig. 1 schematically illustrates the stereochemical structure of the flow cell being applied to liquid on-line detection of the embodiment of the present invention 1
Separate sketch, Fig. 2 schematically illustrates the upper bottom surface of briquetting and the structure of bottom surface, and Fig. 3 schematically illustrates sealing member
Upper bottom surface and the structure of bottom surface (bottom surface of sealing member is identical with upper bottom surface structure), Fig. 4 schematically illustrates support
The upper bottom surface of part and the structure of bottom surface, as Figure 1-4, described flow cell includes：
Briquetting 1, described briquetting is provided with first through hole 11, the second through hole 12 and third through-hole 13, described first through hole be into
Liquid passage, described second through hole is liquid outlet channel；The bottom surface of described briquetting is provided with the first array groove, and described first array is recessed
Groove is embedded with first group of magnetic part 14；
Sealing member 2, described sealing member is provided with fourth hole 21, fifth hole 22, and described first through hole and the second through hole exist
Described sealing member projection in the plane fall in described fourth hole, described first through hole, the second through hole and fourth hole
Constitute the fluid passage of described flow cell；Described sealing member is elastomeric material, positioned between described briquetting and support member；
Support member 3, described support member upper surface is provided with the second array groove, and described second array groove is embedded with second group
Magnetic part 31, described second group of magnetic part is oppositely arranged with first group of magnetic part and attracts each other so that described briquetting is close with described
Sealing, chip of light waveguide form the fluid passage of sealing；Described support member upper surface is additionally provided with the groove placing elastic component 32, institute
The upper surface stating elastic component is higher than the upper surface of described support member；
Chip of light waveguide 4, described chip of light waveguide is provided with sensing area and optical fiber interface, and sensing area is described
Sealing member projection in the plane fall in described fourth hole, described sensing area is fixed with absorption test substance
Modified membrane；Described third through-hole, fifth hole up/down perforation and corresponding with the position of optical fiber interface, pencil optical fiber from described up and down
Stretch out in the passage of insertion；Described chip of light waveguide is located between described elastic component and sealing member, covers described elastic component, and
Its distance at the view field edge of described support member upper surface to described second group of magnetic part is more than zero.
Further, described first through hole and the second through hole are divided into upper and lower two parts, and upper end through-hole aperture is more than lower end
Through-hole aperture, is easy to the connection between feed pathway, liquid outlet channel and liquid line.
Further, the lower end through hole of described first through hole and the second through hole described sealing member projection in the plane
Fall in described fourth hole.
In order to exclude the interference of ambient temperature, improve stability and the accuracy of testing result, therefore：
Further, the side of described support member is provided with installing hole 33, installing hole 34, for inserting heating element heater and thermometric
Element, described support member is aluminum material, to improve heat conductivility.Heating element heater heats to support member, and temperature element will
Temperature signal passes to chip of light waveguide.
In order to shorten the heat time heating time of liquid, preheated before sample enters fluid passage, therefore：
Further, the bottom surface of described support member is provided with temperature chamber, and described temperature chamber is embedded with water influent pipeline, described enter
Fluid passage described in the outlet of liquid pipeline；Described temperature chamber passes through cover plate (as magnetic closure) and seals, and improves temperature chamber
Heat-insulating property.
First group of magnetic part and second group of magnetic part are oppositely arranged and attract each other so that keeping between briquetting and support member
Sealing, in order to ensure the magnetic part existence anduniquess corresponding relation in the magnetic part in first group of magnetic part and second group of magnetic part,
Namely the corresponding relation of briquetting and support member fixes, briquetting will not rotated to when inhaling fixing using magnetic part with support member
Displacement, therefore：
Further, described first group of magnetic part includes the magnetic part of two kinds of polarity, the magnetic in described second group of magnetic part
The opposite polarity of property part and the relative magnetic part in position in first group of magnetic part；
If magnetic part number is 3-5 in first group of magnetic part, the polarity of at least two adjacent magnetic parts is identical；
If in first group of magnetic part magnetic part number be N, N >=6 and be even number, then the pole of at least N/2 adjacent magnetic part
Property is identical；
If in first group of magnetic part magnetic part number be M, M >=7 and for odd number when, then at least (M-1)/2 adjacent magnetic
The polarity of part is identical.
In order to improve the sealing property between briquetting and support member, therefore：
Further, described sealing member at the view field edge of described support member upper surface to described second group of magnetic part
Distance be more than zero so that first group of magnetic part and second group of magnetic part directly contact attract.
Further, described first group of magnetic part and second group of magnetic part uniformly divide along the circumferential direction in respective place face
Cloth；Or, at least two magnetic parts in described first group of magnetic part and/or second group of magnetic part are with regard in described flow cell
Heart axis centre is symmetrical.
In order to exclude the uncertain factors such as temperature, ambient humidity, the chip of light waveguide of the present embodiment is provided with reference region, described
Reference region is without fluid passage；Light source injects described flow cell through pencil optical fiber, is divided into two-way, and a road entrance reference region is formed
Reference light paths；One tunnel entering signal sensing unit forms detection light path.
The present embodiment also provides method of work in liquid on-line detection process for the above-mentioned flow cell, described method of work bag
Include：
(A1) sample enters fluid passage, and component to be measured is adsorbed by the modified membrane in sensing area；
(A2) buffer enters fluid passage and displaces sample；
(A3) light source injects described flow cell, and a road enters reference light paths, and a road enters detection light path；
(A4) reference light paths output reference signal I1, detection light path output detection signal I simultaneously2, according to I2/I1Ratio with
The relation of concentration of component to be measured obtains the content of component to be measured in sample.
Component to be measured in sample detects after being modified film absorption in the environment of buffer, eliminates matrix interference.One
After secondary sample detection terminates, need to be eluted out the component to be measured of absorption, and allow fluid passage, sensing area in buffer
In the environment of preserve, to protect chip of light waveguide, therefore：
Further, described method of work also includes：
(B1) eluent rinses described fluid passage, and eluting is modified the component to be measured of film absorption；
(B2) buffer flows through described fluid passage, displaces eluent.
Embodiment 2
The present embodiment provides a kind of flow cell of the embodiment of the present invention 1 to be applied to the method for work of liquid on-line detection, with
Unlike embodiment 1, the signal that reference light paths are exported by the present embodiment, as temperature indication signal, treats that temperature indication signal is steady
After fixed, detection light path carries out the content of component to be measured in the measurement of control signal and detection signal, and then acquisition sample, tool successively
Body step is as follows：
(C1) buffer enters fluid passage, and light source injects described flow cell, and a road enters reference light paths, and a road enters inspection
Light-metering road；
(C2) using the output signal of reference light paths as temperature indication signal, after temperature indication signal is stable, detection light
Road exports control signal I0；
(C3) sample enters described fluid passage, and component to be measured is adsorbed by described modified membrane；
(C4) buffer enters fluid passage and displaces sample, after temperature indication signal is stable, detection light path output inspection
Survey signal I2；
(C5) according to I2/I0What ratio and concentration of component to be measured closed is the content obtaining component to be measured in sample.
Embodiment 3
The present embodiment provides a kind of flow cell being applied to liquid on-line detection and its method of work, different from embodiment 1
Be：The sensing area of the present embodiment includes at least two subregions, each subregion is fixed with and adsorbs different components to be measured
Modified membrane, detection light path exports the detection signal of each subregion, obtains the content of different components to be measured in sample.
Embodiment 4
The present embodiment is the application examples in water sample on-line checking field for the flow cell of the embodiment of the present invention 1.
In this application examples, the cylinder that briquetting is made for lucite, near the circumference at described briquetting bottom surface edge
On be uniformly arranged 4 grooves, be embedded with the Magnet of N, N, S, S polarity along clockwise direction successively；Support member is aluminum material cylinder
Body, is inserted with heating rod and temperature element in the side installing hole of support member；In the circumference at described support member upper bottom surface edge
It is provided with the one-to-one groove with briquetting bottom surface groove, be embedded with the Magnet of S, S, N, N polarity successively；Bottom on described support member
The centre in face is provided with circular groove, and described circular groove is embedded in the O-ring as elastic component, and the upper surface of described O-ring is high
In the upper bottom surface of support member, chip of light waveguide is played with support and protective effect；Chip of light waveguide is covered on O-ring, institute
The maximum stating the distance to described flow cell central axis for the chip of light waveguide edge is less than described magnet edges in flow cell
The minima of heart axial line distance；Sealing member is elaxtic seal, and described sealing member falls in the projection of described support member upper surface
Enter Magnet to enclose in region；Modified membrane in described chip of light waveguide sensing area is molecular imprinting film, and not subregion is only used for
Detect the Algae toxins in water sample to be measured.
The on-line checking process of water sample is as follows：
S1. the feed pathway of above-mentioned flow cell is connected with water influent pipeline, liquid outlet channel connects waste liquid bottle；Light source through with
Described flow cell injected by the pencil optical fiber that chip of light waveguide is connected, and is divided into two-way, road entrance reference region formation reference light paths, and one
Road entering signal sensing unit forms detection light path；
S2. sample enters fluid passage, and Algae toxins are adsorbed by the molecular imprinting film in sensing area；Heating rod starts to add
Heat；
S3. buffer enters fluid passage and displaces sample；
S4. reference light paths output reference signal I1, detection light path output detection signal I simultaneously2, according to I2/I1Ratio with treat
The relation surveying concentration of component obtains the content of Algae toxins in water sample；
S5. eluent flushing liquid passage, the Algae toxins that eluting is adsorbed；
S6. buffer flows through described fluid passage, displaces eluent, preserves described flow cell under buffer conditions.
Briquetting in the embodiment of the present invention, sealing member, the bottom surface of support member are not limited to circle, rectangle, square, many
Side shape etc. is all within protection scope of the present invention.
Claims (10)
1. a kind of be applied to liquid on-line detection flow cell it is characterised in that：Described flow cell includes：
Briquetting, described briquetting is provided with first through hole, the second through hole and third through-hole, and it is recessed that the bottom surface of briquetting is provided with the first array
Groove, described first array groove is embedded with first group of magnetic part；
Sealing member, described sealing member is provided with fourth hole, fifth hole, and described first through hole and the second through hole are in described sealing member
Projection in the plane fall in described fourth hole, described first through hole, the second through hole and fourth hole constitute described stream
The fluid passage in logical pond；Described sealing member is elastomeric material, positioned between described briquetting and support member；
Support member, described support member upper surface is provided with the second array groove, and described second array groove is embedded with second group of magnetic
Part, described second group of magnetic part and first group of magnetic part be oppositely arranged and attract each other so that described briquetting and described sealing member,
Chip of light waveguide forms the fluid passage of sealing；Described support member upper surface is additionally provided with the groove placing elastic component, described elasticity
The upper surface of part is higher than the upper surface of described support member；
Chip of light waveguide, described chip of light waveguide is provided with sensing area and optical fiber interface, and sensing area is in described sealing
Part projection in the plane fall in described fourth hole, described sensing area is fixed with the modification of absorption test substance
Film；Described third through-hole, fifth hole up/down perforation, pencil optical fiber connects described optical fiber interface, and from described up/down perforation
Stretch out in passage；Described chip of light waveguide is located between described elastic component and sealing member, and it is in described support member upper surface
The distance of view field edge to described second group of magnetic part is more than zero.
2. flow cell according to claim 1 it is characterised in that：The side of described support member is provided with two installing holes, uses
In insertion heating element heater and temperature element.
3. flow cell according to claim 2 it is characterised in that：The bottom surface of described support member is provided with temperature chamber, described
Temperature chamber is embedded with water influent pipeline, fluid passage described in the outlet of described water influent pipeline.
4. flow cell according to claim 1 it is characterised in that：Described first group of magnetic part includes the magnetic of two kinds of polarity
Part, the opposite polarity of the magnetic part in described second group of magnetic part and the relative magnetic part in position in first group of magnetic part；
If magnetic part number is 3-5 in first group of magnetic part, the polarity of at least two adjacent magnetic parts is identical；
If in first group of magnetic part magnetic part number be N, N >=6 and be even number, then the polarity phase of at least N/2 adjacent magnetic part
With；
If magnetic part number is M in first group of magnetic part, M >=7 and during for odd number, then at least (M-1)/2 adjacent magnetic part
Polarity is identical.
5. flow cell according to claim 1 it is characterised in that：Described sealing member is in the projection of described support member upper surface
The distance of edges of regions to described second group of magnetic part is more than zero.
6. according to the arbitrary described flow cell of claim 1-5 it is characterised in that：Described chip of light waveguide further includes：Ginseng
Ratio area, described reference region is without fluid passage；
Light source injects described flow cell through pencil optical fiber, is divided into two-way, and a road entrance reference region forms reference light paths；One tunnel enters
Sensing area forms detection light path.
7. according to claim 6 flow cell method of work it is characterised in that：The method of work of described flow cell includes：
(A1) sample enters fluid passage, and component to be measured is adsorbed by the modified membrane in sensing area；
(A2) buffer enters fluid passage and displaces sample；
(A3) light source injects described flow cell, and a road enters reference light paths, and a road enters detection light path；
(A4) reference light paths output reference signal I1, detection light path output detection signal I simultaneously2, according to I2/I1Ratio with to be measured group
Divide the content of component to be measured in the relation acquisition sample of concentration.
8. method of work according to claim 7 it is characterised in that：Described method of work further includes：
(B1) eluent rinses described fluid passage, and eluting is modified the component to be measured of film absorption；
(B2) buffer flows through described fluid passage, displaces eluent.
9. the method for work according to claim 7 or 8 it is characterised in that：Sensing area includes at least two subregions, institute
State and the modified membrane adsorbing different components to be measured is fixed with least two subregions, detection light path exports the detection letter of each subregion
Number, obtain the content of different components to be measured in sample.
10. according to claim 6 flow cell method of work it is characterised in that：The method of work of described flow cell includes：
(C1) buffer enters fluid passage, and light source injects described flow cell, and a road enters reference light paths, and a road enters detection light
Road；
(C2) using the output signal of reference light paths as temperature indication signal, after temperature indication signal is stable, detection light path is defeated
Go out control signal I0；
(C3) sample enters described fluid passage, and component to be measured is adsorbed by described modified membrane；
(C4) buffer enters fluid passage and displaces sample, after temperature indication signal is stable, detection light path output detection letter
Number I2；
(C5) according to I2/I0What ratio and concentration of component to be measured closed is the content obtaining component to be measured in sample.
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