CN106474421B - A kind of Chinese medicine composition that treating colorectal cancer and its application - Google Patents
A kind of Chinese medicine composition that treating colorectal cancer and its application Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/284—Atractylodes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/39—Convolvulaceae (Morning-glory family), e.g. bindweed
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/64—Orobanchaceae (Broom-rape family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8994—Coix (Job's tears)
Abstract
The invention discloses a kind of Chinese medicine composition for treating the carcinoma of the rectum and its applications, are formulated by the Chinese medicine of following weight ratio: cairo morningglory root or leaf: Rhizoma Atractylodis Macrocephalae: ginseng: Paris polyphylla: Chinese knotweed: curcuma zedoary: Herba Cistanches: semen coicis=(25-35): (50-70): (10-20): (10-20): (25-35): (10-20): (10-20): (25-35).Application of the traditional chinese medicine composition of the invention on the drug of the preparation treatment carcinoma of the rectum has effects that treat CRC, therapeutic effect is good, securely and reliably, and uses Chinese medicine, raw material is easy to get.
Description
Technical field
The present invention relates to field of medicaments, specially a kind of Chinese medicine composition for treating colorectal cancer and its application.
Background technique
Colorectal cancer (Colorectal cancer, CRC) is common one of tumor in digestive tract.American cancer in 2013
Prediction data shows that CRC new cases and death toll are respectively 142820 and 50830, and cancer is newly sent out and death
The 3rd of all kinds of cancers is arranged, about respectively accounts for 9%.In the past 10 years, global colorectal cancer incidence rate and mortality level are substantially steady
It is fixed, but account for global Incidence, dead ratio increased.The Regional Distribution of Colorectal Cancer differs greatly,
Middle developed regions colorectal cancer incidence rate is higher.China is the area Di Fa of colorectal cancer, but disease incidence is in rise year by year trend.It cuts
Only 2010, China's colorectal cancer neopathy number of cases was more than 270,000,130,000 or more death.
CRC belongs to traditional Chinese medicine mass located in the upper or lower abdomen, intestines Bleach, ovarian cyst scope.Traditional Chinese medicine diagnosis and treatment CRC has long history." Plain Questions " is said
" name of disease says mass located in the upper or lower abdomen ", " intestines Bleach defecation with blood and pus ", " cold air visitor is fought with gas phase is defended in parenteral, and gas must not be flourish, because there is direct bearing, addiction and it is interior
Write, bad odor has been that polyp is raw ", illustrate that this disease and eating and drinking without temperance, exopathogen are invaded, battalion defends imbalance, and it is related to injure the five internal organs.Spleen deficiency
Be tumour formed this, Zhang Yuansu " living method confidential " is said: " strengthening people without product, empty people then has it, and weakness of the spleen and the stomach, qi and blood two declines, when four
Thoughts, all can be at product ", Jing-Yue Complete Works are said: " people of spleen kidney deficiency and debilitating disorder has the disease of accumulation more "；During multidigit name is old
Doctor proposes that treatment and prevention of tumour should be with invigorating the spleen for this.
Therefore, with Chinese Traditional Medicine combination Modern preparations technology, a kind of compound Chinese medicinal preparation for treating CRC is researched and developed,
The micro- side of righting tune, has great importance, while also having huge market development potential.
Summary of the invention
The purpose of the present invention is to provide a kind of Chinese medicine composition for treating the carcinoma of the rectum, the micro- side of righting tune is significant in efficacy.
To achieve the goals above, the present invention adopts the following technical scheme: a kind of Chinese medicine composition for treating the carcinoma of the rectum, by
The Chinese medicine of following weight ratio is formulated: cairo morningglory root or leaf: Rhizoma Atractylodis Macrocephalae: ginseng: Paris polyphylla: Chinese knotweed: curcuma zedoary: Herba Cistanches: semen coicis=
(25-35):(50-70):(10-20):(10-20):(25-35):(10-20):(10-20):(25-35)。
Preferably, it is formulated by the Chinese medicine of following weight ratio: cairo morningglory root or leaf: Rhizoma Atractylodis Macrocephalae: ginseng: Paris polyphylla: Chinese knotweed: cowherb
Art: Herba Cistanches: semen coicis=(25-30): (55-65): (15-20): (10-15): (25-30): (10-15): (10-15):
(25-30)。
It is furthermore preferred that being formulated by the Chinese medical extract and Chinese medicine of following weight ratio: cairo morningglory root or leaf: Rhizoma Atractylodis Macrocephalae: ginseng: weight
Building: Chinese knotweed: curcuma zedoary: Herba Cistanches: semen coicis=30:60:15:12:30:12:15:30.
Application of the above-mentioned Chinese medicine composition on the drug of the preparation treatment carcinoma of the rectum.
The dosage form of the drug is decoction, tablet, capsule or granule.
The drug, decoction are to be obtained with the Chinese medicine in the Traditional Chinese medicine composition formula by decocting.
The drug, it is to have that tablet, capsule, granule, which are with the extract of component in the Traditional Chinese medicine composition formula,
Ingredient is imitated, preparation made from pharmaceutic adjuvant is added.
The extract is water extract.
Chinese medicine composition provided by the invention has effects that treatment CRC, and therapeutic effect is good, securely and reliably, and adopts
With Chinese medicine, raw material is easy to get.
Detailed description of the invention
Fig. 1 is the influence in experimental example of the present invention to mouse weight；
Fig. 2 is each group mouse Colon audio-visual picture in experimental example of the present invention；
Fig. 3 is mouse colon tumors number in experimental example of the present invention；
Fig. 4 is that IHC detects influence of the micro- side of righting tune of the present invention to carcinogenic correlative protein expression；
Fig. 5 is that WB detects influence of the micro- side of righting tune of the present invention to carcinogenic correlative protein expression；
Fig. 6 is the CD4+CD25+Foxp3+T cell proportion in experimental example of the present invention in each group mouse peripheral blood；
Fig. 7 is the CD4+CD25+Foxp3+T cell proportion of the spleen of each group mouse in experimental example of the present invention；
Fig. 8 is influence of the WB detection micro- side of righting tune to Foxp3 albumen.
Specific embodiment
The present invention is the Chinese medicine composition (the also known as micro- side of righting tune) for treating CRC, is matched by the Chinese medicine of following weight ratio
It makes: cairo morningglory root or leaf: Rhizoma Atractylodis Macrocephalae: ginseng: Paris polyphylla: Chinese knotweed: curcuma zedoary: Herba Cistanches: semen coicis=(25-35): (50-70): (10-
20):(10-20):(25-35):(10-20):(10-20):(25-35)。
Preferably, it is formulated by the Chinese medicine of following weight ratio: cairo morningglory root or leaf: Rhizoma Atractylodis Macrocephalae: ginseng: Paris polyphylla: Chinese knotweed: cowherb
Art: Herba Cistanches: semen coicis=(25-30): (55-65): (15-20): (10-15): (25-30): (10-15): (10-15):
(25-30)。
It is furthermore preferred that being formulated by the Chinese medicine of following weight ratio: cairo morningglory root or leaf: Rhizoma Atractylodis Macrocephalae: ginseng: Paris polyphylla: Chinese knotweed: cowherb
Art: Herba Cistanches: semen coicis=30:60:15:12:30:12:15:30.
We are using cairo morningglory root or leaf, Rhizoma Atractylodis Macrocephalae as monarch drug in a prescription, strengthening the spleen and replenishing qi thickness stomach；Monarch drug in a prescription air making-up and spleen enlivening is assisted with ginseng, with Paris polyphylla
The clearing heat and detoxicating tumor that disappears is altogether ministerial drug；It is altogether with Herba Cistanches kidney tonifying Wen Yang with Chinese knotweed, curcuma zedoary, semen coicis removing toxic substances promoting blood circulation clearing damp
Assistant makes.All medicines closes and plays invigorating the spleen altogether and disappear the function of tumor.
In Chinese medicine used in the present invention:
Cairo morningglory root or leaf is moraceae plants cleft palm banyan Ficus simplicissima Lour. [F.hirta Vahl
Var.palmatiloba (Merr.) Chun] root.It is sweet in flavor, mild-natured, returns spleen, lung, Liver Channel.With strengthening the spleen and tonifying the lung, promoting the circulation of qi dampness removing,
The effect of relaxing tendons and activating collaterals.For spleen deficiency edema, deficiency of food is powerless, pulmonary tuberculosis cough, night sweat, leukorrhagia, postpartum agalorrhea, rheumatic arthralgia, water
It is swollen, traumatic injury etc..Cairo morningglory root or leaf mainly contains psoralen, 3 beta-hydroxy beans steroid -5- alkene -7- ketone, cupreol, hexadecylic acid, 18
Acid, apiolin, alpha-amyrin acetic acid esters etc..Have the effects that antibacterial, antiviral, anticoagulation, inhibit tumour, immunological regulation.
Rhizoma Atractylodis Macrocephalae is the dry rhizome of composite family perennial herb Rhizoma Atractylodis Macrocephalae Atractylodes macrocephala Koidz..Taste
It is bitter, sweet, warm-natured, returns spleen, stomach meridian.With air making-up and spleen enlivening, eliminate dampness and have diuretic effect, strengthening exterior and reducing sweat, the effect of miscarriage prevention.For anorexia and loose stool,
Abdominal fullness and distention, the soft mind of limb is tired, phlegm retention, oedema, difficult urination, spontaneous perspiration and threatened abortion.Rhizoma Atractylodis Macrocephalae mainly contains volatile oil, main component
For atractylol, atractylone, atractylodes lactone etc..Still containing acetylenic, vitamin A etc..With strong, diuresis, hypoglycemic, liver protection, it is anticoagulant,
It expands blood vessel, decompression, inhibit the effects of bacterium and fungi.
Ginseng is the dry root of Araliaceae perennial herb ginseng Panax ginseng C.A.Mey..Sweet in flavor, slight bitter, property
Tepor, return heart, lung, the spleen channel.Have the function of reinforcing vital energy, reinforces the spleen to benefit the lung, promotes the production of body fluid to quench thirst, tranquilize the mind and promote the intelligence.Be intended to take off for the deficiency of vital energy,
The critical disease of feeble and impalpable pulse, syndrome characterized by dyspnea of losing heart, lazy Yan Shengwei, feeble pulse spontaneous perspiration, fatigue and weakness, anorexia and loose stool are quenched one's thirst, palpitaition, are lost
It sleeps, amnesia etc..Ginseng mainly contains ginsenoside, the volatile oil such as α-panacene, the organic acids such as panax acid, the flavones such as Panasenoside
And lignanoid, sterol, amino acid, polysaccharide etc..With adjusting nervous centralis, enhancing memory, excited hypophysis Sexual gland system, adjust
The effects of blood pressure, blood glucose.
Paris polyphylla is Liliaceae herbaceos perennial Yunnan Rhizoma Paridis Paris polyphylla smith
The rhizome of var.Yunnanensis (Franch.) Hara.Bitter, cold nature, return liver warp.With clearing heat and detoxicating, swelling and pain relieving,
The effect of dispelling wind and relieving convulsion.For carbuncle sore tumefacting virus, worm snake bite, traumatic hemorrhage, pain caused by ecchymoma, liver hot air wind producing, infantile convulsion, epilepsy.Weight
Building-owner will contain paris polyphylla glycosides, Dioscin etc..With antibacterial, antidote against snake bite, relieving cough and asthma, anti-inflammatory, hemostasis, it is antitumor, adjust it is immune
The effects of.
Chinese knotweed is the drying herb of polygonaceae arsesmart Chinese knotweed Polygonum chinense Linn..It is sour, sweet,
It is cold in nature.Return liver, the spleen channel.With clearing heat and promoting diuresis, the effect of removing pattogenic heat from the blood and toxic material from the body.For diarrhea due to damp-heat, dysentery, jaundice, abscess of throat.Fire
Charcoal mother contains Kaempferol, Quercetin, -7 glucoside of Kaempferol, Kaempferol -3- glucuronide, cupreol, inverse galla turcica
Acid, gallic acid, 3-O- methyl benzoaric acid.With antibacterial, contraction intestinal smooth muscle effect.
Curcuma zedoary is zingiberaceous plant zedoary Curcuma phaeocaulis Val., Guangxi zedoary Curcuma
Kwangsiensis S.G.Lee et C.F.Liang or RADIX CURCUMAE Curcuma wenyujin Y.H.Chen et C.Ling
Dry rhizome.Acrid flavour, hardship, it is warm-natured, return liver, the spleen channel.With breaking blood and promoting the circulation of qi, the effect of clearing stagnation and killing pain.For a lump in the abdomen causing distension and pain, close
Through, confidant sting, stagnation of QI due to dyspepsia, abdominal distention, traumatic injury, pain caused by ecchymoma etc..Curcuma zedoary mainly contains volatile oil, main component
For more than the 20 kinds of sequiterpenes such as curcuma zedoary furanone, Curcumenol, curcumene.Still containing palmitinic acid, curcumin etc..With anticancer, enhancing
The effects of immune, antibacterial, antithrombotic.
Herba Cistanches are the perennial meat parasitism draft Herba Cistanches Cistanche deserticola Y.C.Ma's of Orobanchaceae
The fleshy stem of dry zone scale leaf.It is sweet in flavor, salty, it is warm-natured, return kidney, large intestine channel.With kidney-replenishing, benefiting essence-blood, the effect of relax bowel and defecation.
Infertile for impotence, soreness and weakness of waist and knees, muscles and bones is powerless, dry constipation of intestines etc..Herba Cistanches mainly contain glycine betaine, cupreol, carrot
Glycosides, triacontanol, acteoside, mannitol, stearic acid, Salidroside etc..With blood pressure lowering, antiatherosclerosis, tune
Whole endocrine promotes metabolism, enhances the effects of remembering, is strong.
Semen coicis is perennial coix lacryma-jobi Coix lacryma-jobi L.var.Ma-yuen (Roman.) of grass family
The dry mature kernal of Stapf.It is sweet in flavor, light, cold nature, returns spleen, stomach, lung channel.With clearing damp and promoting diuresis, invigorating the spleen to arrest diarrhea, heat-clearing row
Purulence, the effect of eliminating impediment.For oedema, difficult urination, splenasthenic diarrhea, pulmonary abscess, acute appendicitis, arthritis with fixed pain caused by dampness muscle arteries and veins contraction.As long as semen coicis contains
Coix seed oil, semen coicis rouge, protein, fat oil, carbohydrate, vitamin B1, Coixol, sterol, coixan A,
B, C etc..Have the effects that anticancer, antibacterial, solution spasm, hypoglycemic, antipyretic, calm, analgesia.
Application of the above-mentioned Chinese medicine composition on the drug of the preparation treatment carcinoma of the rectum.The dosage form of drug can be decoction, piece
The peroral dosage forms such as agent, capsule or granule.Decoction is to be obtained with the Chinese medicine in the Traditional Chinese medicine composition formula by decocting.
Tablet, capsule, granule are to add pharmaceutic adjuvant using the extract of component in the Traditional Chinese medicine composition formula as effective component
Preparation obtained, it is preferred that the extract is water extract, routinely extraction process by water.
Below with reference to embodiment, the present invention is further illustrated, but the present invention is not limited thereto specific examples.
Embodiment 1
Take cairo morningglory root or leaf 30g, Rhizoma Atractylodis Macrocephalae 60g, ginseng 15g, Paris polyphylla 12g, Chinese knotweed 30g, curcuma zedoary 12g, Herba Cistanches 12g, coix lacryma-jobi
Benevolence 30g is put into medicine pot, and cool drinking water is added, and is impregnated 20-30 minutes, is added water and is not crossed drug, medicine pot is placed on stove fire
It is decocted with moderate heat.After decocting for the first time, medical fluid is poured out (about 150~200 milliliters), then heat appropriate amount of water (if the dregs of a decoction cool again
Water should be cooled when decoction), it carries out second and decocts, medical fluid is poured out (about 150~200 milliliters), liquid medicine mixing twice point 2 times
It takes.
Embodiment 2
Take cairo morningglory root or leaf 660.0g, Rhizoma Atractylodis Macrocephalae 1320.0g, ginseng 330.0g, Paris polyphylla 264.0g, Chinese knotweed 660.0g, curcuma zedoary
264.0g, Herba Cistanches 264.0g, semen coicis 660.0g add 10 times of amount water, impregnate 45min, extract 2 times, each 90min, filtration,
Merging filtrate is concentrated into cream (1.05~1.10,70 DEG C), belt drying, is carried out whole grain after 14 meshes, is then added suitable
Magnesium stearate mixes, is pressed into 1000 to get tablet.Every slice weight 0.45g.
Embodiment 3
Take cairo morningglory root or leaf 660.0g, Rhizoma Atractylodis Macrocephalae 1320.0g, ginseng 330.0g, Paris polyphylla 264.0g, Chinese knotweed 660.0g, curcuma zedoary
264.0g, Herba Cistanches 264.0g, semen coicis 660.0g add 10 times of amount water, impregnate 45min, extract 2 times, each 90min, filtration,
Merging filtrate is concentrated into cream (1.05~1.10,70 DEG C), belt drying, is crushed, and 2% magnesium stearate is added, and is packed into capsule, system
At 1000 to get hard capsule.Every 0.45g containing content.
Embodiment 4
Take cairo morningglory root or leaf 660.0g, Rhizoma Atractylodis Macrocephalae 1320.0g, ginseng 330.0g, Paris polyphylla 264.0g, Chinese knotweed 660.0g, curcuma zedoary
264.0g, Herba Cistanches 264.0g, semen coicis 660.0g add 10 times of amount water, impregnate 45min, extract 2 times, each 90min, filtration,
Merging filtrate is concentrated into cream (1.05~1.10,70 DEG C), and belt drying is ground into fine powder, and suitable dextrin and lactose is added, and mixes
Even, granulation, 65 DEG C of dryings cross 14 meshes and carry out whole grain, and 1000 packets are made to get granule in packing.Every packet weight 5g.
Embodiment 5
Take cairo morningglory root or leaf 550.0g, Rhizoma Atractylodis Macrocephalae 1210.0g, ginseng 330.0g, Paris polyphylla 220.0g, Chinese knotweed 550.0g, curcuma zedoary
Tablet is made by 2 preparation method of embodiment in 220.0g, Herba Cistanches 220.0g, semen coicis 550.0g.
Embodiment 6
Take cairo morningglory root or leaf 660.0g, Rhizoma Atractylodis Macrocephalae 1430.0g, ginseng 440.0g, Paris polyphylla 330.0g, Chinese knotweed 660.0g, curcuma zedoary
Tablet is made by 2 preparation method of embodiment in 330.0g, Herba Cistanches 330.0g, semen coicis 660.0g.
Embodiment 7
Take cairo morningglory root or leaf 616.0g, Rhizoma Atractylodis Macrocephalae 1320.0g, ginseng 396.0g, Paris polyphylla 286.0g, Chinese knotweed 616.0g, curcuma zedoary
Tablet is made by 2 preparation method of embodiment in 286.0g, Herba Cistanches 286.0g, semen coicis 616.0g.
Embodiment 8
Take cairo morningglory root or leaf 550.0g, Rhizoma Atractylodis Macrocephalae 1100.0g, ginseng 220.0g, Paris polyphylla 220.0g, Chinese knotweed 550.0g, curcuma zedoary
Tablet is made by 2 preparation method of embodiment in 220.0g, Herba Cistanches 220.0g, semen coicis 550.0g.
Embodiment 9
Take cairo morningglory root or leaf 770.0g, Rhizoma Atractylodis Macrocephalae 1540.0g, ginseng 440.0g, Paris polyphylla 440.0g, Chinese knotweed 770.0g, curcuma zedoary
Tablet is made by 2 preparation method of embodiment in 440.0g, Herba Cistanches 440.0g, semen coicis 770.0g.
Experimental example (the micro- side of righting tune studies the therapeutic effect of CRC)
1. material
1.1 animal: C57BL/6J-ApcMin/ J (abbreviation Apcmin/+) strain height easily spontaneously forms enteron aisle adenoma.Homozygote
Mouse cannot survive.Apcmin/+Mouse is bought from model animal research institute, Nanjing University and in pharmaceutical college, Nanfang Medical Univ SPF
Animal house propagating and breeding breeds 30 8 week old Apcmin/+Mouse.15 are separately bought from Nanfang Medical Univ experimental animal 8 weeks
SPF grades of male C 57 BL/6 J mouses are raised in SPF grades of animal houses of pharmaceutical college, Nanfang Medical Univ.Experiment records mouse before starting
Weight and number.
1.2 reagents: embodiment 1 is pressed, the micro- side of righting tune is by cairo morningglory root or leaf 30g, Rhizoma Atractylodis Macrocephalae 60g, ginseng 15g, Paris polyphylla 12g, live charcoal
Female 30g, curcuma zedoary 12g, Herba Cistanches 12g, semen coicis 30g composition, all purchased from attached Nanfang Hospital, Nanfang Medical Univ.Righting tune
Micro- side uses traditional water decoction method, decocts twice.Drug is put into medicine pot, cool drinking water is added, impregnates 20-30 minutes, adds
Water did not had drug, and medicine pot is placed on electric furnace and is decocted.After decocting for the first time, medical fluid is poured out (about 150~200 milliliters), then plus
Appropriate warm water (if the dregs of a decoction, which cool, should cool water when decocting again) carries out second and decocts, and medical fluid is poured out (about 150~200 millis
Rise), liquid medicine mixing twice, 4 DEG C of packing is saved backup.
1.3 instruments:
2. method
2.1 groupings and administration: experimental animal is divided into 3 groups, wild control group (Wild Type) 15,30 Apcmin/+It is small
Mouse is randomly divided into two groups, Apcmin/+Group 15, Apcmin/+The micro- side's group of+righting tune 15.Wherein, wild control group (Wild
Type) mouse and Apcmin/+Group mouse is fed in a conventional manner as after component cage.Apcmin/+The micro- side's group mouse of+righting tune is pressed
It is given in a manner of stomach-filling according to 10ml/kg/d standard and prepares spare medical fluid.
2.2 samplings:
One week adaptation environment is normally raised after experiment mice grouping, freer feed and drinking-water, and second week starts to be administered.
Apcmin/+The micro- side's group of+righting tune gives the micro- side of righting tune (10ml/kg/d) stomach-filling.Monitored weekly during experiment mouse weight and into
Appetite variation, the symptoms such as observation mouse general state such as hair, activity and hematochezia, de- tripe, and record dead mouse situation.
Terminate experiment after being administered 8 weeks, after mouse fasting for 24 hours, weigh in and record, blood is taken using eyeball, blood is stood
After 2h, 3000r/min is centrifuged 15min, takes supernatant, is sub-packed in EP pipe and saves the detection for being used for the subsequent cell factor for -80 DEG C.So
Mouse is put to death using cervical dislocation afterwards, mouse is fixed on ice, its abdominal cavity is cut off, is taken by anus mouth to cap end separation
Under whole section of Colon and rectum, split colon along the intestinal tube longitudinal axis, clean intestinal contents with the physiological saline of pre-cooling.The knot that will be cleaned up
Intestinal mucosa is laid in up on clean filter paper, has observed and recorded tumor free generation and its number, size and location, meter
Calculate Tumor incidence.Remaining physiological saline in colonic tissue is blotted with clean filter paper, colonic tissue is divided into several etc.
Part, wherein the colonic tissue for pathological study puts into fixed in 10% neutral formalin solution, remaining tissue packing immediately
- 80 DEG C of preservations in cryopreservation tube, are used for subsequent experimental study.
2.3 statistical procedures: all data are with mean ± standard deviationIt indicates, statistical procedures use spss22.0
Software is analyzed, and has statistical difference as P < 0.05.
3 results
Influence of the 3.1 micro- sides of righting tune to CRC mouse weight:
After three group of 8 week old mouse is handled 8 weeks according to dosage regimen, wild control group (Wild Type) mores than 15, Apcmin/+
Group Yus 8, Apcmin/+The micro- Fang Zuyu of+righting tune 12.Apcmin/+Have when group 8 week old of mouse with C57BL/6J mouse weight bright
Significant difference is different, and difference is increased as week old increases.Apcmin/+After the micro- side's group of+righting tune is administered 3 weeks, weight and Apcmin/+Group
There are significant difference, the result is shown in Figure 1s for mouse.Therefore, righting tune is micro- can be obviously improved Apcmin/+The weight of intestinal cancer mouse.
Influence of the 3.2 micro- sides of righting tune to CRC mouse Colon:
At the end of experiment, mouse Colon is taken out, the colon of Wild Type group mouse does not find tumour, Apcmin/+Group and
Apcmin/+The micro- side's group mouse of+righting tune generates tumour, sees Fig. 2.Compared to an Apcmin/+Group mouse, Apcmin/+The micro- side of+righting tune
The tumor number of group mouse is less, as a result sees Fig. 3.Therefore, righting tune is micro- can substantially reduce Apcmin/+The colon of+mouse is swollen
Tumor.
The micro- side of 3.3 righting tune reduces the expression of the carcinogenic GAP-associated protein GAP of CRC mouse
β-catenin, Cox-2, p53, PCNA are representational carcinogenic GAP-associated protein GAP.Immunohistochemical method
(IHC) as the result is shown: Apcmin/+β-catenin, Cox-2, p53, PCNA in group mouse intestinal tissue express opposite Wild
Type group dramatically increases (P < 0.01), and Apcmin/+β-catenin, Cox-2 in the micro- side's group mouse intestinal tissue of+righting tune,
Apc has been compared in p53, PCNA expressionmin/+Group significantly reduces (P < 0.01), as a result sees Fig. 4.Immunoblotting (WB) result with exempt from
Epidemic disease histochemical method (IHC) result is similar, as a result sees Fig. 5.Therefore, the micro- carcinogenic related egg that can lower CRC mouse of righting tune
White expression.
The 3.4 micro- sides of righting tune play antitumaous effect by immunological regulation
NTrey cell (CD4+CD25+Foxp3+) is regulatory T cells (Treg), there is maintenance tumor patient to be immunized resistance to
The effect received, has a very important significance in antineoplaston.Foxp3 is mainly expressed in thymus gland, spleen and lymph node etc.
Lymphoid organ and tissue.It is specific expressed in CD4+CD25+T cell in mouse, without being expressed in CD8+T cell.In the mankind
Foxp3 can not only be expressed in CD4+CD25+T cell, can also be expressed in CD8+CD28-T cell.But the table in CD4+T cell
Up to being apparently higher than CD8+T cell.Currently, Foxp3 (forkhead box P3, Scurfin) is Treg cell generally acknowledged at present
Most sensitive mark.
The CD4+CD25+Foxp3+T cell discovery for detecting mouse peripheral blood and spleen, compares Wild Type group mouse,
Apcmin/+CD4+CD25+Foxp3+T cell proportion in group mouse intestinal tissue dramatically increases (P < 0.01), and Apcmin/++ righting
Adjust the CD4+CD25+Foxp3+T cell proportion in micro- side's group mouse intestinal tissue with respect to Apcmin/+Group significantly reduces (P < 0.01),
As a result see Fig. 6, Fig. 7.It is found using the Foxp3 albumen of immunoblotting (WB) detection mouse intestinal tissue similar as a result, knot
Fruit sees Fig. 8.Prove that the micro- side of righting tune by nTrey cell, influences immunological regulation, to play antitumaous effect.
4. conclusion
The micro- side of righting tune adjusts and is immunized, the expression of carcinogenic GAP-associated protein GAP is lowered, to play improvement by nTrey cell
Intestinal cancer mouse constitution and antitumaous effect.
Claims (5)
1. a kind of application of Chinese medicine composition on the drug that preparation treats the carcinoma of the rectum by nTrey cell-mediated immune, special
Sign is: the Chinese medicine composition is formulated by the Chinese medical extract or Chinese medicine of following weight ratio: cairo morningglory root or leaf: Rhizoma Atractylodis Macrocephalae:
Ginseng: Paris polyphylla: Chinese knotweed: curcuma zedoary: Herba Cistanches: semen coicis=30:60:15:12:30:12:15:30.
2. application according to claim 1, it is characterised in that: the dosage form of the drug be decoction, tablet, capsule or
Granule.
3. application according to claim 2, it is characterised in that: the drug, decoction are in the Chinese medicine composition
Chinese medicine obtained by decocting.
4. application according to claim 2, it is characterised in that: the drug, tablet, capsule, granule are with institute
The extract for stating Chinese medicine composition is effective component, adds preparation made from pharmaceutic adjuvant.
5. application according to claim 4, it is characterised in that: the extract is water extract.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1539458A (en) * | 2003-10-30 | 2004-10-27 | 吉林省辽源亚东药业股份有限公司 | Chinese materia medica preparation made from gen-seng and astragalus root for strengthening the body resistance and anti cancer as well as producing method |
| CN1745843A (en) * | 2005-10-17 | 2006-03-15 | 北京阜康仁生物制药科技有限公司 | Anticancer Chinese medicine compound preparation and its making method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1539458A (en) * | 2003-10-30 | 2004-10-27 | 吉林省辽源亚东药业股份有限公司 | Chinese materia medica preparation made from gen-seng and astragalus root for strengthening the body resistance and anti cancer as well as producing method |
| CN1745843A (en) * | 2005-10-17 | 2006-03-15 | 北京阜康仁生物制药科技有限公司 | Anticancer Chinese medicine compound preparation and its making method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 岭南中药龙术消瘤方对结直肠癌免疫抑制微环境的影响;靖林林等;《新中医》;20010320;第46卷(第7期);第141页左栏第1段、倒数第2段 * |
| 龙术消瘤方降低HIF-1α介导的调节性T细胞抑制APCMin/+小鼠结肠腺瘤生成;易志勇;《中国优秀硕士学位论文全文数据库医药卫生科技辑》;20160331(第3期);第1-3页 * |
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