CN106474387B

CN106474387B - Antibacterial microcapsule for piglets

Info

Publication number: CN106474387B
Application number: CN201611061812.2A
Authority: CN
Inventors: 张艳雯; 郑一民; 张宁; 兰宗宝
Original assignee: Nanning Institute
Current assignee: Boon Group Co., Ltd
Priority date: 2016-11-25
Filing date: 2016-11-25
Publication date: 2019-12-31
Anticipated expiration: 2036-11-25
Also published as: CN106474387A

Abstract

The invention relates to the technical field of veterinary biological products, in particular to a microcapsule for piglet antibiosis; the composition comprises the following raw materials in parts by weight: 15-20 parts of bacillus amyloliquefaciens, 5-10 parts of a traditional Chinese medicine reinforcing agent, 2-3 parts of kaolin, 5-7 parts of magnesium aluminum hydroxide, 8-12 parts of ethyl cellulose, 6-9 parts of methyl cellulose, 2-4 parts of butyl hydroxy anisole and 90-100 parts of ultrapure water; the microcapsule disclosed by the invention can stimulate the generation of immunocompetence of piglets, help the piglets to establish an intestinal microecological system as early as possible, prevent the damage of the digestive tract structure of the piglets, prevent the piglets from being invaded by fungi, viruses and pathogenic microorganisms, prevent the stress reaction of the piglets, improve the stress resistance and disease resistance of the piglets, regulate the organism metabolism, effectively reduce the morbidity and mortality, obviously reduce the diarrhea diseases of the piglets and improve the growth performance of the piglets.

Description

Antibacterial microcapsule for piglets

Technical Field

The invention relates to the technical field of veterinary biological products, and in particular relates to a piglet antibacterial microcapsule.

Background

With the continuous improvement of the large-scale breeding level, the early weaning of piglets is more and more common, the productivity of sows and the utilization rate of colony house equipment can be improved, the maternal propagation of diseases can be reduced, the growth performance and the later growth effect of the piglets can be improved, however, the problems of appetite reduction, dyspepsia, diarrhea caused diseases and the like are often caused due to the immature gastrointestinal system after the weaning of the piglets, and finally the growth inhibition is expressed; after weaning, the immune system of the piglets is not developed completely, the immunity is low, the piglets are very easy to be invaded by pathogenic bacteria, fungi and viruses, the morbidity is greatly improved, and the survival rate of the piglets is low.

The bacillus amyloliquefaciens can secrete various digestive enzymes and antibacterial substances in the metabolic process, can inhibit pathogenic bacteria, fungi, viruses and the like, can improve the digestive absorption of animals on nutrient substances, maintain the balance of intestinal flora of livestock and poultry, inhibit the growth of harmful pathogenic bacteria and enhance the capability of resisting diseases, and at present, the bacillus amyloliquefaciens as biological control bacteria is widely applied to plant protection and has better inhibiting effect on plant diseases and insect pests; the piglet is a prerequisite guarantee of the whole pig breeding industry, so that researchers develop a novel preparation capable of resisting bacteria, sterilizing and improving the survival rate of the piglet according to the growth requirements of the piglet through the physiological characteristics of the piglet, the novel preparation has important significance for the pig breeding industry, and a new thought is provided for the research of antibacterial property and disease resistance in the livestock breeding industry.

Disclosure of Invention

The invention provides a microcapsule for piglet antibiosis to solve the technical problems.

The method is realized by the following technical scheme:

the antibacterial microcapsule for the piglets comprises the following raw materials in parts by weight: 15-20 parts of bacillus amyloliquefaciens, 5-10 parts of a traditional Chinese medicine reinforcing agent, 2-3 parts of kaolin, 5-7 parts of magnesium aluminum hydroxide, 8-12 parts of ethyl cellulose, 6-9 parts of methyl cellulose, 2-4 parts of butyl hydroxy anisole and 90-100 parts of ultrapure water;

the preparation method of the microcapsule for piglet antibiosis comprises the following steps:

(1) treating the bacillus amyloliquefaciens: inoculating bacillus amyloliquefaciens strains to a culture medium, after 24-48 hours, adding 1 colony into a triangular flask containing 1L of the culture medium, placing the flask in a shaking table for shaking culture overnight, centrifuging for 15-20 min, discarding supernatant, uniformly mixing precipitates by using double distilled water, concentrating by using a rotary evaporator, passing through a bacterial filter of 0.22 mu m, standing for 2-3 hours, centrifuging, discarding supernatant, dissolving precipitates by using ultrapure water, adding sodium alginate of which the mass is 2-3 times of that of the precipitates, concentrating by using the rotary evaporator, passing through a bacterial filter of 0.22 mu m, standing for 1-2 hours, centrifuging, and discarding supernatant;

(2) preparing a traditional Chinese medicine reinforcing agent: weighing the Chinese herbal medicines, respectively cleaning, mashing to 40-50 min, adding ethyl acetate, soaking at 70 ℃ for 3-4 h, recovering ethyl acetate under reduced pressure, filtering, reserving residual liquid for later use, adding water into filter residues, decocting, filtering, repeating for 2 times, decocting 1.5-2 h each time, combining the three filtrates and the residual liquid, and decocting to obtain an extract with the water content of 16-20%;

(3) preparing an inner core: mixing the bacillus amyloliquefaciens in the step (1) and the extract in the step (2), drying until the water content is 2-3%, and mixing the mixture and the sterilized kaolin according to the mass ratio of 9-11: 0.9-1.1 to obtain an inner core;

(4) preparing microcapsules: and (3) mixing the magnesium aluminum hydroxide, the ethyl cellulose, the methyl cellulose, the butyl hydroxy anisole and the ultrapure water according to a ratio, uniformly stirring, adding the inner core prepared in the step (3), stirring at the rotating speed of 30-35 r/min for 20-30 min, and drying.

Preferably, the feed consists of the following raw materials in parts by weight: bacillus amyloliquefaciens 18, a traditional Chinese medicine reinforcing agent 8, kaolin 2.6, magnesium aluminum hydroxide 6, ethyl cellulose 10, methyl cellulose 8, butyl hydroxy anisol 3 and ultrapure water 95.

Further, the traditional Chinese medicine reinforcing agent is composed of the following raw materials in parts by weight: 20-30 parts of folium artemisiae argyi, 6-9 parts of bletilla striata, 10-15 parts of fructus forsythiae, 2-3 parts of dandelion, 20-25 parts of endothelium corneum gigeriae galli, 10-15 parts of mangnolia officinalis, 4-7 parts of fingered citron, 15-20 parts of rehmannia, 3-5 parts of cortex mori radicis, 6-9 parts of radix asparagi, 2-3 parts of buffalo horn, 7-11 parts of radix ophiopogonis, 5-9 parts of sweet wormwood, 1-3 parts of Chinese lobelia, 5-10 parts of semen coicis, 2-4 parts of phili.

Further, the culture medium is composed of the following raw materials in parts by weight: 10-15 parts of agar, 10-15 parts of beef soup, 8-12 parts of glucose, 1-2 parts of magnesium chloride, 1-3 parts of potassium hydrogen phosphate, 1-2 parts of magnesium sulfate, 1-2 parts of sodium chloride, 6-9 parts of thiamine and 4-7 parts of sodium acetate.

Further, the centrifugation is carried out at the rotating speed of 2000-3000 r/min.

Further, the concentration of the sodium alginate is 80-100 mmol/L.

Further, the disinfected kaolin is prepared by removing impurities from kaolin, putting the kaolin in a pot at the temperature of 60-80 ℃, stir-frying for 15-20 min, soaking, and then treating for 10-15 min by using a microwave sterilization machine.

Furthermore, the antimicrobial microcapsule for the piglets is fed to the piglets along with the feed, and the microcapsule and the piglet feed are mixed according to the mass ratio of 0.2-0.5 to 1 before feeding.

In conclusion, the beneficial effects of the invention are as follows: the microcapsule disclosed by the invention can stimulate the generation of immunocompetence of piglets, help the piglets to establish an intestinal microecological system as early as possible, prevent the damage of the digestive tract structure of the piglets, prevent the piglets from being invaded by fungi, viruses and pathogenic microorganisms, prevent the stress reaction of the piglets, improve the stress resistance and disease resistance of the piglets, regulate the organism metabolism, effectively reduce the morbidity and mortality, obviously reduce the diarrhea diseases of the piglets and improve the growth performance of the piglets.

The bacillus amyloliquefaciens is used as a raw material, can effectively secrete various digestive enzymes and antibacterial substances, can inhibit pathogenic bacteria, fungi, viruses and the like, can improve the digestive absorption of animals on nutrient substances, maintain the balance of intestinal flora of livestock and poultry, inhibit the growth of harmful pathogenic bacteria and enhance the disease resistance.

By adding the traditional Chinese medicine reinforcing agent, the development of an immune system of an organism, the digestion and absorption of intestines and stomach and the secretion of growth hormone are effectively promoted, meanwhile, the antibacterial and antiviral effects can be achieved, the immunity of the organism is improved, the invasion of external pathogenic microorganisms, bacteria and fungi can be resisted, the growth of piglets is promoted, and the morbidity and the mortality are reduced.

Detailed Description

The following is a detailed description of the embodiments of the present invention, but the present invention is not limited to these embodiments, and any modifications or substitutions in the basic spirit of the embodiments are included in the scope of the present invention as claimed in the claims.

Example 1

The antibacterial microcapsule for the piglets comprises the following raw materials in parts by weight: 15kg of bacillus amyloliquefaciens, 5kg of a traditional Chinese medicine reinforcing agent, 2kg of kaolin, 5kg of magnesium aluminum hydroxide, 8kg of ethyl cellulose, 6kg of methyl cellulose, 2kg of butyl hydroxy anisol and 90kg of ultrapure water;

(1) treating the bacillus amyloliquefaciens: inoculating bacillus amyloliquefaciens strains to a culture medium, adding 1 colony into a triangular flask containing 1L of the culture medium after 24 hours, placing the flask in a shaking table for shaking culture overnight, centrifuging for 15min, discarding the supernatant, uniformly mixing the precipitate with double distilled water, concentrating by using a rotary evaporator, passing through a bacterial filter of 0.22 mu m, standing for 2 hours, centrifuging, discarding the supernatant, dissolving the precipitate with ultrapure water, adding sodium alginate of which the mass is 2 times that of the precipitate, concentrating by using the rotary evaporator, passing through the bacterial filter of 0.22 mu m, standing for 1 hour, centrifuging, and discarding the supernatant;

(2) preparing a traditional Chinese medicine reinforcing agent: weighing Chinese herbal medicines, respectively cleaning, smashing for 40min, adding ethyl acetate, soaking at 70 deg.C for 3h, recovering ethyl acetate under reduced pressure, filtering, collecting the residual liquid, decocting the residue with water, filtering, repeating for 2 times (1.5 h each time), mixing the three filtrates and residual liquid, and decocting to obtain extract with water content of 16%;

(3) preparing an inner core: mixing the bacillus amyloliquefaciens in the step (1) and the extract in the step (2), drying until the water content is 2-3%, and mixing the mixture and the disinfected kaolin according to the mass ratio of 9: 0.9 to obtain an inner core;

(4) preparing microcapsules: mixing magnesium aluminum hydroxide, ethyl cellulose, methyl cellulose, butyl hydroxy anisole and ultrapure water according to a proportion, adding the inner core prepared in the step (3) after uniformly stirring, stirring for 20min at the rotating speed of 30r/min, and drying.

The traditional Chinese medicine reinforcing agent is prepared from the following raw materials in parts by weight: 20kg of folium artemisiae argyi, 6kg of bletilla striata, 10kg of fructus forsythiae, 2kg of dandelion, 20kg of endothelium corneum gigeriae galli, 10kg of mangnolia officinalis, 4kg of fingered citron, 15kg of rehmannia glutinosa, 3kg of cortex mori, 6kg of radix asparagi, 2kg of buffalo horn, 7kg of radix ophiopogonis, 5kg of sweet wormwood herb, 1kg of Chinese lobelia herb, 5kg of semen coicis, 2kg of herba violae and 10kg of ligusticum chuanxiong hort.

The culture medium comprises the following raw materials in parts by weight: 10kg of agar, 10kg of beef soup, 8kg of glucose, 1kg of magnesium chloride, 1kg of potassium hydrogen phosphate, 1kg of magnesium sulfate, 1kg of sodium chloride, 6kg of thiamine and 4kg of sodium acetate.

The centrifugation is carried out at the rotating speed of 2000 r/min.

The concentration of the sodium alginate is 80 mmol/L.

The sterilized kaolin is prepared by removing impurities from kaolin, parching at 60 deg.C for 15min, soaking, and treating with microwave sterilizer for 10 min.

The microcapsule for piglet antibiosis is fed to piglets along with feed, and the microcapsule and the piglet feed are mixed according to the mass ratio of 0.2 to 1 before feeding.

Example 2

The antibacterial microcapsule for the piglets comprises the following raw materials in parts by weight: 20kg of bacillus amyloliquefaciens, 10kg of traditional Chinese medicine reinforcing agent, 3kg of kaolin, 7kg of magnesium aluminum hydroxide, 12kg of ethyl cellulose, 9kg of methyl cellulose, 4kg of butyl hydroxy anisol and 100kg of ultrapure water;

(1) treating the bacillus amyloliquefaciens: inoculating bacillus amyloliquefaciens strains to a culture medium, adding 1 colony into a triangular flask containing 1L of the culture medium after 48 hours, placing the flask in a shaking table for shaking culture overnight, centrifuging for 20min, discarding the supernatant, uniformly mixing the precipitate with double distilled water, concentrating by using a rotary evaporator, passing through a bacterial filter of 0.22 mu m, standing for 3 hours, centrifuging, discarding the supernatant, dissolving the precipitate with ultrapure water, adding sodium alginate with the mass 3 times of that of the precipitate, concentrating by using the rotary evaporator, passing through the bacterial filter of 0.22 mu m, standing for 2 hours, centrifuging, and discarding the supernatant;

(2) preparing a traditional Chinese medicine reinforcing agent: weighing Chinese herbal medicines, respectively cleaning, smashing for 50min, adding ethyl acetate, soaking at 70 deg.C for 4h, recovering ethyl acetate under reduced pressure, filtering, collecting the residual liquid, decocting the residue with water, filtering, repeating for 2 times (2 h each time), mixing the three filtrates and residual liquid, and decocting to obtain extract with water content of 20%;

(3) preparing an inner core: mixing the bacillus amyloliquefaciens in the step (1) and the extract in the step (2), drying until the water content is 3%, and mixing the mixture and the disinfected kaolin according to the mass ratio of 11: 1.1 to obtain an inner core;

(4) preparing microcapsules: mixing magnesium aluminum hydroxide, ethyl cellulose, methyl cellulose, butyl hydroxy anisole and ultrapure water according to a proportion, adding the inner core prepared in the step (3) after uniformly stirring, stirring for 30min at the rotating speed of 35r/min, and drying.

The traditional Chinese medicine reinforcing agent is prepared from the following raw materials in parts by weight: 30kg of folium artemisiae argyi, 9kg of bletilla striata, 15kg of fructus forsythiae, 3kg of dandelion, 25kg of endothelium corneum gigeriae galli, 15kg of mangnolia officinalis, 7kg of fingered citron, 20kg of rehmannia glutinosa, 5kg of cortex mori, 9kg of radix asparagi, 3kg of buffalo horn, 11kg of radix ophiopogonis, 9kg of sweet wormwood herb, 3kg of Chinese lobelia herb, 10kg of semen coicis, 4kg of herba violae and 15kg of ligusticum chuanxiong hort.

The culture medium comprises the following raw materials in parts by weight: 15kg of agar, 15kg of beef soup, 12kg of glucose, 2kg of magnesium chloride, 3kg of potassium hydrogen phosphate, 2kg of magnesium sulfate, 2kg of sodium chloride, 9kg of thiamine and 7kg of sodium acetate.

And the rotation speed of the centrifugation is 3000 r/min.

The concentration of the sodium alginate is 100 mmol/L.

The sterilized kaolin is prepared by removing impurities from kaolin, parching at 80 deg.C for 20min, soaking, and treating with microwave sterilizer for 15 min.

The microcapsule for piglet antibiosis is fed to piglets along with feed, and the microcapsule and the piglet feed are mixed according to the mass ratio of 0.5 to 1 before feeding.

Example 3

The antibacterial microcapsule for the piglets comprises the following raw materials in parts by weight: 18kg of bacillus amyloliquefaciens, 8kg of a traditional Chinese medicine reinforcing agent, 2.6kg of kaolin, 6kg of magnesium aluminum hydroxide, 10kg of ethyl cellulose, 8kg of methyl cellulose, 3kg of butyl hydroxy anisol and 95kg of ultrapure water;

(1) treating the bacillus amyloliquefaciens: inoculating bacillus amyloliquefaciens strains to a culture medium, adding 1 colony into a triangular flask containing 1L of the culture medium after 26 hours, placing the flask in a shaking table for shaking culture overnight, centrifuging for 18min, discarding supernatant, uniformly mixing precipitates by using double distilled water, concentrating by using a rotary evaporator, passing through a bacterial filter of 0.22 mu m, standing for 2.5 hours, centrifuging, discarding supernatant, dissolving the precipitates by using ultrapure water, adding sodium alginate of which the mass is 2.5 times that of the precipitates, concentrating by using the rotary evaporator, passing through the bacterial filter of 0.22 mu m, standing for 1.5 hours, centrifuging, and discarding supernatant;

(2) preparing a traditional Chinese medicine reinforcing agent: weighing Chinese herbal medicines, respectively cleaning, mashing to 45min, adding ethyl acetate, soaking at 70 deg.C for 3.5h, recovering ethyl acetate under reduced pressure, filtering, collecting the residual solution, decocting the residue with water, filtering, repeating for 2 times (1.8 h each time), mixing the three filtrates and residual solution, and decocting to obtain extract with water content of 18%;

(3) preparing an inner core: mixing the bacillus amyloliquefaciens in the step (1) and the extract in the step (2), drying until the water content is 2.5%, and mixing the mixture and the disinfected kaolin according to the mass ratio of 10: 1 to obtain an inner core;

(4) preparing microcapsules: mixing magnesium aluminum hydroxide, ethyl cellulose, methyl cellulose, butyl hydroxy anisole and ultrapure water according to a proportion, adding the inner core prepared in the step (3) after uniformly stirring, stirring for 25min at the rotating speed of 33r/min, and drying.

The traditional Chinese medicine reinforcing agent is prepared from the following raw materials in parts by weight: 25kg of folium artemisiae argyi, 8kg of bletilla striata, 13kg of fructus forsythiae, 2.5kg of dandelion, 23kg of endothelium corneum gigeriae galli, 13kg of mangnolia officinalis, 5kg of fingered citron, 18kg of rehmannia, 4kg of cortex mori, 8kg of radix asparagi, 2.5kg of buffalo horn, 9kg of radix ophiopogonis, 7kg of sweet wormwood herb, 2kg of Chinese lobelia, 8kg of semen coicis, 3kg of Chinese violet and 13kg of ligusticum chuanxiong hort.

The culture medium comprises the following raw materials in parts by weight: 13kg of agar, 13kg of beef soup, 10kg of glucose, 1.5kg of magnesium chloride, 2kg of potassium hydrogen phosphate, 1.5kg of magnesium sulfate, 1.5kg of sodium chloride, 8kg of thiamine and 5kg of sodium acetate.

And the rotation speed of the centrifugation is 2500 r/min.

The concentration of the sodium alginate is 90 mmol/L.

The sterilized kaolin is prepared by removing impurities from kaolin, parching at 70 deg.C for 18min, soaking, and treating with microwave sterilizer for 13 min.

The microcapsule for piglet antibiosis is fed to piglets along with feed, and the microcapsule and the piglet feed are mixed according to the mass ratio of 0.35 to 1 before feeding.

Example 4

The antibacterial microcapsule for the piglets comprises the following raw materials in parts by weight: 20kg of bacillus amyloliquefaciens, 5kg of a traditional Chinese medicine reinforcing agent, 3kg of kaolin, 5kg of magnesium aluminum hydroxide, 12kg of ethyl cellulose, 6kg of methyl cellulose, 4kg of butyl hydroxy anisol and 90kg of ultrapure water;

(1) treating the bacillus amyloliquefaciens: inoculating bacillus amyloliquefaciens strains to a culture medium, adding 1 colony into a triangular flask containing 1L of the culture medium after 24 hours, placing the flask in a shaking table for shaking culture overnight, centrifuging for 20min, discarding the supernatant, uniformly mixing the precipitate with double distilled water, concentrating by using a rotary evaporator, passing through a bacterial filter of 0.22 mu m, standing for 2 hours, centrifuging, discarding the supernatant, dissolving the precipitate with ultrapure water, adding sodium alginate with the mass 3 times of that of the precipitate, concentrating by using the rotary evaporator, passing through the bacterial filter of 0.22 mu m, standing for 1 hour, centrifuging, and discarding the supernatant;

(2) preparing a traditional Chinese medicine reinforcing agent: weighing Chinese herbal medicines, respectively cleaning, smashing for 40min, adding ethyl acetate, soaking at 70 deg.C for 3h, recovering ethyl acetate under reduced pressure, filtering, collecting the residual liquid, decocting the residue with water, filtering, repeating for 2 times (2 h each time), mixing the three filtrates and residual liquid, and decocting to obtain extract with water content of 16%;

(3) preparing an inner core: mixing the bacillus amyloliquefaciens in the step (1) and the extract in the step (2), drying until the water content is 3%, and mixing the mixture and the disinfected kaolin according to the mass ratio of 11: 0.9 to obtain an inner core;

(4) preparing microcapsules: mixing magnesium aluminum hydroxide, ethyl cellulose, methyl cellulose, butyl hydroxy anisole and ultrapure water according to a proportion, adding the inner core prepared in the step (3) after uniformly stirring, stirring for 20min at the rotating speed of 35r/min, and drying.

The traditional Chinese medicine reinforcing agent is prepared from the following raw materials in parts by weight: 30kg of folium artemisiae argyi, 6kg of bletilla striata, 10kg of fructus forsythiae, 3kg of dandelion, 20kg of endothelium corneum gigeriae galli, 15kg of mangnolia officinalis, 4kg of fingered citron, 20kg of rehmannia glutinosa, 3kg of cortex mori, 9kg of radix asparagi, 2kg of buffalo horn, 7kg of radix ophiopogonis, 9kg of sweet wormwood herb, 3kg of Chinese lobelia herb, 5kg of semen coicis, 4kg of herba violae and 15kg of ligusticum chuanxiong hort.

The culture medium comprises the following raw materials in parts by weight: 15kg of agar, 10kg of beef soup, 8kg of glucose, 2kg of magnesium chloride, 1kg of potassium hydrogen phosphate, 1kg of magnesium sulfate, 2kg of sodium chloride, 9kg of thiamine and 7kg of sodium acetate.

The centrifugation is carried out at the rotating speed of 2000 r/min.

The concentration of the sodium alginate is 80 mmol/L.

The sterilized kaolin is prepared by removing impurities from kaolin, putting the kaolin in a pot at 60 ℃, stir-frying for 15min, soaking in the kaolin, and treating the kaolin for 15min by using a microwave sterilization machine.

Example 5

The antibacterial microcapsule for the piglets comprises the following raw materials in parts by weight: 20kg of bacillus amyloliquefaciens, 10kg of traditional Chinese medicine reinforcing agent, 2kg of kaolin, 5kg of magnesium aluminum hydroxide, 12kg of ethyl cellulose, 9kg of methyl cellulose, 2kg of butyl hydroxy anisol and 100kg of ultrapure water;

(1) treating the bacillus amyloliquefaciens: inoculating bacillus amyloliquefaciens strains to a culture medium, adding 1 colony into a triangular flask containing 1L of the culture medium after 48 hours, placing the flask in a shaking table for shaking culture overnight, centrifuging for 20min, discarding the supernatant, uniformly mixing the precipitate with double distilled water, concentrating by using a rotary evaporator, passing through a bacterial filter of 0.22 mu m, standing for 2 hours, centrifuging, discarding the supernatant, dissolving the precipitate with ultrapure water, adding sodium alginate with the mass 3 times of that of the precipitate, concentrating by using the rotary evaporator, passing through the bacterial filter of 0.22 mu m, standing for 1 hour, centrifuging, and discarding the supernatant;

(2) preparing a traditional Chinese medicine reinforcing agent: weighing Chinese herbal medicines, respectively cleaning, smashing for 50min, adding ethyl acetate, soaking at 70 deg.C for 3h, recovering ethyl acetate under reduced pressure, filtering, collecting the residual liquid, decocting the residue with water, filtering, repeating for 2 times, repeating for 2h, mixing the three filtrates and residual liquid, and decocting to obtain extract with water content of 16%;

(3) preparing an inner core: mixing the bacillus amyloliquefaciens in the step (1) and the extract in the step (2), drying until the water content is 2-3%, and mixing the mixture and the disinfected kaolin according to the mass ratio of 11: 1.1 to obtain an inner core;

The traditional Chinese medicine reinforcing agent is prepared from the following raw materials in parts by weight: 30kg of folium artemisiae argyi, 9kg of bletilla striata, 15kg of fructus forsythiae, 2kg of dandelion, 20kg of endothelium corneum gigeriae galli, 10kg of mangnolia officinalis, 4kg of fingered citron, 20kg of rehmannia glutinosa, 5kg of cortex mori, 9kg of radix asparagi, 2kg of buffalo horn, 7kg of radix ophiopogonis, 5kg of sweet wormwood herb, 3kg of Chinese lobelia, 10kg of semen coicis, 4kg of herba violae and 15kg of ligusticum chuanxiong.

The culture medium comprises the following raw materials in parts by weight: 15kg of agar, 15kg of beef soup, 8kg of glucose, 1kg of magnesium chloride, 1kg of potassium hydrogen phosphate, 2kg of magnesium sulfate, 2kg of sodium chloride, 6kg of thiamine and 7kg of sodium acetate.

And the rotation speed of the centrifugation is 3000 r/min.

The concentration of the sodium alginate is 100 mmol/L.

The sterilized kaolin is prepared by removing impurities from kaolin, parching at 60 deg.C for 20min, soaking, and treating with microwave sterilizer for 10 min.

The microcapsule for piglet antibiosis is fed to piglets along with feed, and the microcapsule and the piglet feed are mixed according to the mass ratio of 0.4 to 1 before feeding.

Examples of the experiments

1. Selecting a plurality of healthy, disease-free and weaned piglets, wherein the male and female halves of the piglets are used as research objects and are respectively divided into 6 groups, the first five groups are respectively added with the same amount of the microcapsules prepared in the examples 1-5 into the feed, the last group is used as a blank group, and the feed is not added with the microcapsules; the following statistics were made for the condition of piglets, and the results are shown in table 1:

TABLE 1

Item	The incidence of disease%	Survival rate%	The daily gain%
				Example 1	8.4	93.2	21.2
Example 2	8.2	93.4	21.8
				Example 3	7.9	93.6	22.3
Example 4	8.3	93.3	23.8
				Example 5	8.4	92.9	20.1
Blank group	22.3	84.9	——

The daily gain is the proportion of the gain on the basis of the blank group.

Claims

1. The antibacterial microcapsule for the piglets is characterized by being prepared from the following raw materials in parts by weight: 15-20 parts of bacillus amyloliquefaciens, 5-10 parts of a traditional Chinese medicine reinforcing agent, 2-3 parts of kaolin, 5-7 parts of magnesium aluminum hydroxide, 8-12 parts of ethyl cellulose, 6-9 parts of methyl cellulose, 2-4 parts of butyl hydroxy anisole and 90-100 parts of ultrapure water;

(2) preparing a traditional Chinese medicine reinforcing agent: weighing the Chinese herbal medicines, respectively cleaning, mashing for 40-50 min, adding ethyl acetate, soaking at 70 ℃ for 3-4 h, recovering ethyl acetate under reduced pressure, filtering, reserving residual liquid for later use, adding water into filter residues, decocting, filtering, repeating for 2 times, decocting for 1.5-2 h each time, combining the three filtrates and the residual liquid, and decocting to obtain an extract with the water content of 16-20%;

(4) preparing microcapsules: mixing magnesium aluminum hydroxide, ethyl cellulose, methyl cellulose, butyl hydroxy anisole and ultrapure water according to a ratio, uniformly stirring, adding the inner core prepared in the step (3), stirring at the rotating speed of 30-35 r/min for 20-30 min, and drying;

the traditional Chinese medicine reinforcing agent is prepared from the following raw materials in parts by weight: 20-30 parts of folium artemisiae argyi, 6-9 parts of bletilla striata, 10-15 parts of fructus forsythiae, 2-3 parts of dandelion, 20-25 parts of endothelium corneum gigeriae galli, 10-15 parts of mangnolia officinalis, 4-7 parts of fingered citron, 15-20 parts of rehmannia, 3-5 parts of cortex mori radicis, 6-9 parts of radix asparagi, 2-3 parts of buffalo horn, 7-11 parts of radix ophiopogonis, 5-9 parts of sweet wormwood, 1-3 parts of Chinese lobelia, 5-10 parts of semen coicis, 2-4 parts;

centrifuging at the rotating speed of 2000-3000 r/min;

the concentration of the sodium alginate is 80-100 mmol/L;

the disinfected kaolin is obtained by removing impurities from kaolin, putting the kaolin in a pot at the temperature of 60-80 ℃, stir-frying for 15-20 min, and then treating for 10-15 min by using a microwave sterilization machine;

the feed is fed to piglets along with the feed, and the microcapsule and the piglet feed are mixed according to the mass ratio of 0.2-0.5 percent to 1 before feeding.

2. The piglet antibacterial microcapsule according to claim 1, which is prepared from the following raw materials in parts by weight: bacillus amyloliquefaciens 18, a traditional Chinese medicine reinforcing agent 8, kaolin 2.6, magnesium aluminum hydroxide 6, ethyl cellulose 10, methyl cellulose 8, butyl hydroxy anisol 3 and ultrapure water 95.

3. The piglet antibacterial microcapsule according to claim 1, wherein the culture medium is prepared from the following raw materials in parts by weight: 10-15 parts of agar, 10-15 parts of beef soup, 8-12 parts of glucose, 1-2 parts of magnesium chloride, 1-3 parts of potassium hydrogen phosphate, 1-2 parts of magnesium sulfate, 1-2 parts of sodium chloride, 6-9 parts of thiamine and 4-7 parts of sodium acetate.