CN106472307A - A kind of isolated culture base of Billbergiapyramidalis - Google Patents
A kind of isolated culture base of Billbergiapyramidalis Download PDFInfo
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- CN106472307A CN106472307A CN201610874240.3A CN201610874240A CN106472307A CN 106472307 A CN106472307 A CN 106472307A CN 201610874240 A CN201610874240 A CN 201610874240A CN 106472307 A CN106472307 A CN 106472307A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
A kind of isolated culture base of Billbergiapyramidalis, including three kinds of culture medium of different phase, the raw material of various culture medium and its constituent concentration are as follows, and Initial culture base is：On improvement White medium base, first the concentration of iron sulfate is adjusted to 3.0～3.2mg/L, the concentration of sodium sulfate is adjusted to 160～180mg/L, then add aspartic acid and heteroauxing；Subculture medium is：On improvement White medium base, first the concentration of boric acid is adjusted to 2.0～2.2mg/L, the concentration of molybdenum oxide is adjusted to 0.0003～0.0005mg/L, in addition add vitamin K1, heteroauxing and naphthalene acetic acid again；Root media is：In B5On medium base, in addition add ammonium molybdate and gibberellins again, adjusting pH with lime water is 7.2.Culture medium of the present invention is directed to Billbergiapyramidalis growth characteristic and designs, and disclosure satisfy that the nutritional need in each stage of Billbergiapyramidalis tissue culture.
Description
Technical field
The present invention relates to technical field of tissue culture, the isolated culture base of specifically a kind of Billbergiapyramidalis.
Background technology
Billbergiapyramidalis (Billbergia pyramidalis Lindl.) pineapple family perennial evergreen draft succulent, stem
Very short.The wealthy lanceolar of leaf, anxious point, there are serration, hard leather matter, emerald green in edge, and there is thick horny layer on surface and absorbs scale.Spike
Inflorescence is upright, exceeds leafage, bract pink, corolla vermilion, rolls up, boundary zone purple outside petal.Blade revolves folded at rhizome
Shape is grown thickly, and base portion is in rosette-stape, and center is cylindrical in shape.Bloom more than winter-spring season.Blade leathery；Verdant and gloss, grows thickly into lotus throne
Shape is dignified beautiful；The mutual obvolvent of leaf base, makes plant center become tubular, interior being filled with water and do not leak, shape is like water tower, therefore " water of gaining the name
Tower is spent ".Like warm, moistening, half shade environment.Can not resist cold.Slightly drought-enduring, originate in Brazil.Billbergiapyramidalis strain Cong Qingcui, pattern is gorgeous, is
Good pot flowers.Billbergiapyramidalis in full bloom is the good merchantable brand interspersing balcony, room, plants foliage plant for good indoor room.Former
Produce american torrid zone, grow nonparasitically upon another plant on the tree of Tropical forests or in humus.There are cultivation, especially southern area more in Chinese the greenhouse.So
And, the traditional nursery propagation method yield reduction of Billbergiapyramidalis, virus accumulation is serious, variety deterioration, quality and yield significantly under
Fall, planting benefit declines.Group culturation rapid propagating technology is applied as economic, efficient propagation method on a lot of plants, cycle is short,
Breeding coefficient is high, low cost, not time and space restricted.
Content of the invention
The present invention is directed to the deficiencies in the prior art, meets artificial fast breeding, the needs of large area high-yield cultivating, provides one
Plant the isolated culture base of Billbergiapyramidalis.
To achieve these goals, present invention employs technical scheme below：
A kind of isolated culture base of Billbergiapyramidalis is it is characterised in that include three kinds of culture medium of different phase, various culture medium
Raw material and its constituent concentration as follows,
Initial culture base is：On improvement White medium base, first the concentration of iron sulfate is adjusted to 3.0～
3.2mg/L, the concentration of sodium sulfate is adjusted to 160～180mg/L, then adds aspartic acid 6～8mg/L, adds heteroauxing 2
～3mg/L；
Subculture medium is：On improvement White medium base, first the concentration of boric acid is adjusted to 2.0～2.2mg/
L, the concentration of molybdenum oxide is adjusted to 0.0003～0.0005mg/L, in addition adds vitamin K1 0.5～0.8mg/L again, adds
Heteroauxing 1～1.2mg/L, adds naphthalene acetic acid 1.2～1.5mg/L；
Root media is：In B5On medium base, in addition add ammonium molybdate 0.01～0.02mg/L again, add red mould
Plain 2～3mg/L, adjusting pH with lime water is 7.2.
Compared with prior art, the beneficial effect that the present invention possesses：
Culture medium of the present invention is directed to Billbergiapyramidalis growth characteristic and designs, and disclosure satisfy that each stage of Billbergiapyramidalis tissue culture
Nutritional need, carries out tissue culture using isolated culture base of the present invention to Billbergiapyramidalis, and survival rate is high, the Billbergiapyramidalis children of acquisition
Seedling stalwartness vitality is indomitable, can rapidly adapt to natural growthing condition, effectively improve the breeding coefficient of Billbergiapyramidalis, be after transplanting
Billbergiapyramidalis is commercially produced provides sufficient seedling source.Compared with seed propagation, tissue culture method effectively can shorten growing-seedling period,
Can continuously produce in a large number indoors, realize batch production, specialized and scale, improve the culture survival rate of seedling, improve
Yield and quality.
Specific embodiment
Below by embodiment, technical scheme is further elaborated.
Embodiment 1
A kind of isolated culture base of Billbergiapyramidalis, including three kinds of culture medium of different phase, the raw material of various culture medium and its
Constituent concentration is as follows,
Initial culture base is：On improvement White medium base, first the concentration of iron sulfate is adjusted to 3.2mg/L, will
The concentration of sodium sulfate is adjusted to 180mg/L, then adds aspartic acid 8mg/L, adds heteroauxing 3mg/L；
Subculture medium is：On improvement White medium base, first the concentration of boric acid is adjusted to 2.2mg/L, by oxygen
The concentration changing molybdenum is adjusted to 0.0005mg/L, in addition adds vitamin K1 0.8mg/L again, adds heteroauxing 1.2mg/L, adds
Plus naphthalene acetic acid 1.5mg/L；
Root media is：In B5On medium base, in addition add ammonium molybdate 0.02mg/L again, add gibberellins 3mg/
L, adjusting pH with lime water is 7.2.
Embodiment 2
A kind of isolated culture base of Billbergiapyramidalis, including three kinds of culture medium of different phase, the raw material of various culture medium and its
Constituent concentration is as follows,
Initial culture base is：On improvement White medium base, first the concentration of iron sulfate is adjusted to 3.0mg/L, will
The concentration of sodium sulfate is adjusted to 160mg/L, then adds aspartic acid 6mg/L, adds heteroauxing 2mg/L；
Subculture medium is：On improvement White medium base, first the concentration of boric acid is adjusted to 2.0mg/L, by oxygen
The concentration changing molybdenum is adjusted to 0.0003mg/L, in addition adds vitamin K1 0.5mg/L again, adds heteroauxing 1mg/L, adds
Naphthalene acetic acid 1.2mg/L；
Root media is：In B5On medium base, in addition add ammonium molybdate 0.01mg/L again, add gibberellins 2mg/
L, adjusting pH with lime water is 7.2.
Embodiment 3
A kind of isolated culture base of Billbergiapyramidalis, including three kinds of culture medium of different phase, the raw material of various culture medium and its
Constituent concentration is as follows,
Initial culture base is：On improvement White medium base, first the concentration of iron sulfate is adjusted to 3.0～
3.2mg/L, the concentration of sodium sulfate is adjusted to 160～180mg/L, then adds aspartic acid 6～8mg/L, adds heteroauxing 2
～3mg/L；
Subculture medium is：On improvement White medium base, first the concentration of boric acid is adjusted to 2.0～2.2mg/
L, the concentration of molybdenum oxide is adjusted to 0.0003～0.0005mg/L, in addition adds vitamin K1 0.5～0.8mg/L again, adds
Heteroauxing 1～1.2mg/L, adds naphthalene acetic acid 1.2～1.5mg/L；
Root media is：In B5On medium base, in addition add ammonium molybdate 0.01～0.02mg/L again, add red mould
Plain 2～3mg/L, adjusting pH with lime water is 7.2.
Embodiment 4
A kind of method Billbergiapyramidalis cultivated using isolated culture base of the present invention, is comprised the steps：
(1) obtain explant and sterilize
The newborn young tender sprout of the healthy and strong plant of selection, water washing twig surface smut dust from the beginning, first alcohol-pickled, then rise
Hydrargyrum is sterilized, and be sterilized after last aseptic water washing is clean explant,
(2) initial culture
Sterilization explant is divided into the stem with bud of 0.6～1.0cm, then accesses and in Initial culture base, cultivate 32～36
It obtains adventitious bud,
Initial culture condition is：Temperature is 28 ± 2 DEG C, and humidity is 55～60%, and light application time is 8～10 hours/day, light
Be 1800～20001x according to intensity, initial culture the 1st～7 day, daily apply ultrasonic field 3～4 hours, ultrasonic field power is 10～
15W/cm2, ultrasonic field frequencies range is 25～28KHz, initial culture the 8th～15 day, daily applying ultrasonic field 4～6 hours, ultrasonic field
Power is 20～22W/cm2, ultrasonic field frequencies range is 30～35KHz；
Initial culture base is：On improvement White medium base, first the concentration of iron sulfate is adjusted to 3.1mg/L, will
The concentration of sodium sulfate is adjusted to 170mg/L, then adds aspartic acid 7mg/L, adds heteroauxing 2.5mg/L；
(3) successive transfer culture
Adventitious bud is transferred to culture in subculture medium and obtains successive transfer culture Seedling within 40～45 days,
Successive transfer culture condition is：Temperature is 30 ± 2 DEG C, and humidity is 65～70%, and light application time is 12～15 hours/day,
Intensity of illumination is 3000～35001x,
Subculture medium is：On improvement White medium base, first the concentration of boric acid is adjusted to 2.1mg/L, by oxygen
The concentration changing molybdenum is adjusted to 0.0004mg/L, in addition adds vitamin K1 0.6mg/L again, adds heteroauxing 1.1mg/L, adds
Plus naphthalene acetic acid 1.3mg/L；
(4) root culture
Successive transfer culture Seedling is transferred to culture in root media and obtains Billbergiapyramidalis seedling within 20～25 days,
Root culture condition is：Temperature is 30 ± 2 DEG C, and humidity is 40～50%, and light application time is 12～15 hours/day,
Intensity of illumination is 5000～60001x,
Root media is：In B5On medium base, in addition add ammonium molybdate 0.015mg/L again, add gibberellins
2.5mg/L, adjusting pH with lime water is 7.2；
(5) seedling exercising and transplanting
After root culture terminates, open culture bottle cap, carry out opening experienced Seedling 5～7 days under natural light, then by Billbergiapyramidalis
Seedling clear water multiple wash and remove residual culture medium, soaks after root 30min clear water again with the carbendazim of mass fraction 3% clear
Move in the little basin of plastics after washing, the little basin of plastics is placed on 2～3 weeks in the booth of shading rate 60%～70%, temperature 25 in booth
～28 DEG C, it is 75～85% that spray sprinkler keeps humidity, and booth seedling exercising is transplanted after one month to field planting ground.
Claims (1)
1. a kind of Billbergiapyramidalis isolated culture base it is characterised in that include different phase three kinds of culture medium, various culture medium
Raw material and its constituent concentration are as follows,
Initial culture base is：On improvement White medium base, first the concentration of iron sulfate is adjusted to 3.0～3.2mg/L,
The concentration of sodium sulfate is adjusted to 160～180mg/L, then adds aspartic acid 6～8mg/L, add heteroauxing 2～3mg/L；
Subculture medium is：On improvement White medium base, first the concentration of boric acid is adjusted to 2.0～2.2mg/L, will
The concentration of molybdenum oxide is adjusted to 0.0003～0.0005mg/L, in addition adds vitamin K1 0.5～0.8mg/L again, adds indole
Acetic acid 1～1.2mg/L, adds naphthalene acetic acid 1.2～1.5mg/L；
Root media is：In B5On medium base, in addition add ammonium molybdate 0.01～0.02mg/L again, add gibberellins 2～
3mg/L, adjusting pH with lime water is 7.2.
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| CN201610874240.3A CN106472307B (en) | 2016-10-05 | 2016-10-05 | A kind of in vitro culture base of Billbergiapyramidalis |
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| CN201610874240.3A CN106472307B (en) | 2016-10-05 | 2016-10-05 | A kind of in vitro culture base of Billbergiapyramidalis |
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| CN106472307A true CN106472307A (en) | 2017-03-08 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385430A (en) * | 2008-09-25 | 2009-03-18 | 天津滨海大顺花卉科技发展股份有限公司 | Pineapple flower forcing and nourishing method |
| CN104542306A (en) * | 2015-02-02 | 2015-04-29 | 广西壮族自治区药用植物园 | Fast propagation method of longans |
-
2016
- 2016-10-05 CN CN201610874240.3A patent/CN106472307B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385430A (en) * | 2008-09-25 | 2009-03-18 | 天津滨海大顺花卉科技发展股份有限公司 | Pineapple flower forcing and nourishing method |
| CN104542306A (en) * | 2015-02-02 | 2015-04-29 | 广西壮族自治区药用植物园 | Fast propagation method of longans |
Non-Patent Citations (1)
| Title |
|---|
| 王春荣等: "红藻凤梨的离体快速繁殖", 《植物生理学通讯》 * |
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Effective date of registration: 20221213 Address after: 276800 R&D Building, No. 8, Electronic Information Industrial Park, Gaoxin 7th Road, Rizhao High tech Zone, Shandong Province (No. 237, Xueyuan Road, Rizhao High tech Zone, Shandong Province) Patentee after: Rizhao Xinrui Investment Promotion Development Co.,Ltd. Address before: 530105 Guangxi Nanning Guangxi-ASEAN Economic and Technological Development Zone No.98 Qiaoguang Road Da Mingzhu Residential Quarter 2 1 Unit 1001 Rooms Patentee before: NANNING SUICONG FUYU TECHNOLOGY DEVELOPMENT Co.,Ltd. |