CN106472220A - Fructus Pruni pseudocerasi anti-root knot stock screening technique - Google Patents
Fructus Pruni pseudocerasi anti-root knot stock screening technique Download PDFInfo
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- CN106472220A CN106472220A CN201610890568.4A CN201610890568A CN106472220A CN 106472220 A CN106472220 A CN 106472220A CN 201610890568 A CN201610890568 A CN 201610890568A CN 106472220 A CN106472220 A CN 106472220A
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- fructus pruni
- pruni pseudocerasi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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Abstract
The present invention relates to a kind of screening technique of cherry rootstock, more particularly to a kind of Fructus Pruni pseudocerasi anti-root knot stock screening technique.Concretely comprise the following steps：1）, Fructus Pruni pseudocerasi anti-root knot stock screening spring the previous year, in Fructus Pruni pseudocerasi nursery unit, select, buy band crown gall Fructus Pruni pseudocerasi nursery stock, planted in plot；In spring in next year, the band tumor Fructus Pruni pseudocerasi Seedling planted is dug out, soil must not be ploughed deeply；2）, the cherry rootstock tested will be needed to carry out cutting lateral root, plant in above-mentioned plot, plant line-spacing and step 1）Line-spacing of planting identical；After fall foliage, lifting, clean root system, the sense root knot situation of investigation stock, thus screen anti-root knot cherry rootstock.The present invention utilizes the field propagation law of Fructus Pruni pseudocerasi root knot, produces soil environment of carrying disease germs, and to cherry rootstock natural infection, can more accurately reflect the resistance situation to root knot for the stock field, the stock of screening has more production application and is worth.
Description
Technical field
The present invention relates to a kind of screening technique of cherry rootstock, more particularly to a kind of screening of Fructus Pruni pseudocerasi anti-root knot stock
Method.
Background technology
Root knot is a kind of worldwide phytobacterial disease, is to pass through plant roots or stem by the Agrobacterium tumefaciens in soil
Wound invasion after formed, content in soil for this bacterium is extremely abundant, all has generation all over the world, and host is suitable
Extensively, 93 section 331 can be infected and belong to 643 kinds of higher plants, in China, peach tree, Fructus Pruni pseudocerasi, many planting fruit-trees such as Fructus Vitis viniferae are critically ill by crown gall
Evil is especially serious with Fructus Pruni pseudocerasi.Fundamentally to solve the generation of Fructus Pruni pseudocerasi root knot, it is crucial for cultivating Resistant rootstock.
At present, the domestic screening operation having carried out Fructus Pruni pseudocerasi Resistant rootstock, such as Zhang Xiaoming（Zhang Xiaoming, Zhou Yu, opens the spring.Cherry
Radix Persicae carninomatosis water planting authentication method [J]. fruit tree, 2005（3）：51-52）Make use of water culture that cherry rootstock is sieved
Choosing.Fang Hongjun etc. utilizes tissue culture plant inoculation Fructus Pruni pseudocerasi crown gall bacterium, to screen Resistant rootstock.(Fang Hongjun, Wang Guanlin, Wang Huoxu.
Cherry by Introducing Antibacterial Polypeptide Genes dwarfing rootstock obtains the transfer-gen plant [J] of anti-root knot. Botany Gazette, 1999,41 (11):
1192-1198), two methods are required for first isolating and purifying root knot pathogen, then carry out artificial vaccination.Fructus Pruni pseudocerasi Rooted Cuttings manage
Cumbersome, need to configure nutritional solution and need timing to change.The management of Fructus Pruni pseudocerasi tissue cultured seedling is easier than Rooted Cuttings, but to cherry rootstock
Before anti-root knot screening, first to obtain the tissue cultured seedling of this stock, previous work amount is big.And two methods are all using artificial vaccination,
With some differences of field occurrence regularity of stock it is impossible to accurate response stock in field the resistance situation to Fructus Pruni pseudocerasi root knot.
Content of the invention
The invention provides a kind of anti-root knot stock screening technique of Fructus Pruni pseudocerasi, the method utilizes the field of Fructus Pruni pseudocerasi root knot to pass
Broadcast rule, produce soil environment of carrying disease germs, to cherry rootstock natural infection, can more accurately reflect stock field to crown gall
The resistance situation of disease, the stock of screening has more production application and is worth.
The Fructus Pruni pseudocerasi anti-root knot stock screening technique of the present invention, it is characterized in that and comprises the following steps：
1st, experimental field prepare
In spring the previous year of Fructus Pruni pseudocerasi anti-root knot stock screening, in Fructus Pruni pseudocerasi nursery unit, select, buy band crown gall Fructus Pruni pseudocerasi nursery stock,
Planted in plot, the scale being needed according to Fructus Pruni pseudocerasi anti-root knot stock screening test, determine planting area；
In spring in next year, the band tumor Fructus Pruni pseudocerasi Seedling planted is dug out, soil must not be ploughed deeply；
Described band root nodule Fructus Pruni pseudocerasi nursery stock is according to seeding row spacing（0.2-0.4）m*（0.4-0.6）M is planted；
2nd, test is implemented
The cherry rootstock needing to be tested is carried out cutting lateral root, plants in above-mentioned plot, plant line-spacing and step 1）'s
Line-spacing of planting is identical；
In described plot in addition to cultivation experiment cherry rootstock, also plant and have comparison stock, that is, more disease-resistant horse Harry stock（Morbidity
Rate is less than 5%）Be easier to susceptible cherry anvil king's stock（Sickness rate is more than 90%）Compare, one sickness rate of both stocks
Relatively low, one is that sickness rate is higher, has both stocks to compare, and both the anti-crown gall of comparative test stock, can test again simultaneously
The reliability of card method, if that is, cherry anvil king does not fall ill, illustrates that a method is not right, is free from germs, during planting inside ground
The same Routine Management of other control measures；
After fall foliage, lifting, clean root system, the sense root knot situation of investigation stock, thus screen anti-root knot cherry rootstock；
3rd, experimental field subsequent treatment
As also needed to carry out the screening of Fructus Pruni pseudocerasi anti-root knot stock, later test, can continue to carry out in this block, the test period is one
Year；
If off-test, do not recycle this block ground, Ze Yaoduiquan garden carries out sterilization treatment；
During described sterilization treatment, processed using the bacterial agents of going out such as copper sulfate, Thiodiazole-copper, interval, after 7-10 days, enriches life
Organic fertilizer.
The present invention is propagated by nursery stock according to the propagation law of Fructus Pruni pseudocerasi root knot, i.e. distance, and short distance is passed through to irrigate, applied
The approach such as fertilizer, continuous crop are propagated, and pathogenic bacteria is to be entered by wound, make full use of the rule that Fructus Pruni pseudocerasi root knot occurs and propagates
Rule makes fungus in soil, produces the field conditions occurring beneficial to Fructus Pruni pseudocerasi root knot, and cherry rootstock is carried out with anti-crown gall screening.Examination
, compared with Fructus Pruni pseudocerasi water culture and tissue culture plant inoculation method, operation, management are simple, and result of the test more can accurately reflect stock for proved recipe method
Field resistance.Using anti-root knot stock horse Harry below 5% for the sickness rate, and sickness rate easily feels root more than 90%
Carninomatosis stock cherry anvil king, this two stocks as comparison, both anti-crown gall of comparative test stock, again can verification method simultaneously
Reliability, during agricultural planting, the anti-crown gall Fructus Pruni pseudocerasi anvil that filtered out using the Fructus Pruni pseudocerasi of the present invention anti-root knot stock screening technique
Wood, more can react the resistance situation in field to Fructus Pruni pseudocerasi root knot for the stock, have very high using value.
Specific embodiment
Below the embodiment it is clear that described is clearly and completely described to the technical scheme in the embodiment of the present invention
It is only a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
All other embodiment that art personnel are obtained under the premise of not having creative work, broadly falls into the scope of protection of the invention.
Embodiment 1
A kind of Fructus Pruni pseudocerasi anti-root knot stock screening technique of the present embodiment, comprises the following steps：
1st, experimental field prepare
In spring the previous year of Fructus Pruni pseudocerasi anti-root knot stock screening, in Fructus Pruni pseudocerasi nursery unit, select, buy band crown gall Fructus Pruni pseudocerasi nursery stock,
Plant a piece of plot according to seeding row spacing 0.3m × 0.5m, the scale being needed according to Fructus Pruni pseudocerasi anti-root knot stock screening test, really
Determine planting area；
In spring in next year, the band tumor Fructus Pruni pseudocerasi Seedling planted is dug out, soil must not be ploughed deeply；
2nd, test is implemented
13 kinds of cherry rootstocks that needs are tested（Depending on the kind of cherry rootstock, quantity can need according to actual tests）,
Often test 30 plants of kind, carry out cutting lateral root, plant in above-mentioned plot, row 0.3m × 0.5m is originally planted in behavior of planting；
Kind of planting is as follows：
Horse Harry stock, cherry anvil king's stock, excellent lobule, Gisela 6, preferred Folium Isatidiss, examine spy, Demi that, GiselaNo.5,
Great Ye is big, the red tassel of sharp mouth, cherry, Nanking cherry, northeast mountain cherry；
Horse Harry, European and American countries commonly used sweet, sour cherry stock, test Seedling is quoted from northwest
University of agriculture and forestry science and technology university, using seminal propagation；Cherry anvil king, the spring in 2000 is through Laiyang Shandong Province city Lv Ge Mr. Zhuan Zhengao
Introduced by Japanese friend,
Easily cutting propagation.Test nursery stock is cutting propagation；
Excellent lobule, the microphyll type strain selected from cherry, using mound layering；
GiselaNo.5 and Gisela 6, quoted from Shandong Fruit-tree Inst., tissue culture propagation；
Preferably Folium Isatidiss, are the kinds that Yantai academy of agricultural sciences selects from cherry, blade is relatively
Common Folium Isatidiss are thick, and color is green.Test nursery stock is mound layering；
Examine spy, Britain's east cyclopentadienyl woods experimental station campstool moral × cherry selection cross becomes.Root system is sent out
Reach, strong adaptability, drought resisting, admittedly property are good, and plant branch is many, grafting affinity is strong, nursery stock gives birth to
Long healthy and strong, there is preferable yielding ability and yield stability, test nursery stock is mound layering；
Wear Mil, the stock that Yantai academy of agricultural sciences introduces from Belgium, using seminal propagation；
Great Ye is big, select from cherry a kind, and blade area is common Folium Isatidiss
4 times, mound layering；
The red cherry of sharp mouth, is the cherry rootstock that Yimeng area is selected, using mound layering；Cherry, also known as little Fructus Pruni pseudocerasi or careless cherry
Fructus Persicae, species is a lot, typically adopts great Ye in production
Type cherry, typically adopts planting of layer breeding；
Nanking cherry, tree body is undershrub, strong stress resistance, and soil is required with not strict, barren-resistant energy
Power is strong, drought resisting, drought resisting but, in Yantai production useful Nanking cherry poor with Prunus avium grafting affinity
Do base anvil, Fructus Pruni salicinae does intermediate stock grafting Prunus avium.Test stock is seminal propagation；Northeast mountain cherry, Liaoning Province commonly uses cherry
One of Peach rootstock, has that winter resistance is strong, seed is easily bred
The features such as.Test seedling wooden is from Dalian academy of agricultural sciences, seminal propagation.
Wherein, horse Harry stock susceptible gene is less than 5%, and the susceptible gene of cherry anvil king's stock is more than 90%, both stocks
One sickness rate is relatively low, and one is that sickness rate is higher, has both stocks to compare, both the anti-crown gall of comparative test stock,
Again can verification method simultaneously reliability, if that is, cherry anvil king does not fall ill, it is not right to illustrate to send out method, is free from germs inside ground；
After fall foliage, lifting, clean root system, investigate the sense root knot situation of above-mentioned 13 kinds of stocks, thus screening anti-crown gall
Sick cherry rootstock, observes by the naked eye and can be seen that whether stock is susceptible.
As shown in table 1, horse Harry stock susceptible gene is 0% to result, illustrates that horse Harry stock is immunity to Fructus Pruni pseudocerasi root knot
's.This result is consistent with the result of study of Cai Yuliang.（Cai Yuliang, Feng Ying, Zhang Xue etc..Prunus avium new stock horse Harry
The selection-breeding of CDR-1.Journal of Fruit Science, 2013,30（1）：177-178）.The susceptible gene of other stocks is respectively：Excellent lobule 3.6%, Ji
It is 9.1%, preferred Folium Isatidiss 10.0% that plug draws No. 6, examine special 10.7%, your 12.2%, GiselaNo.5 19.2% of Demi, great Ye are big
29.6%th, the sharp red tassel of mouth 30.0%, cherry 35.7%, Nanking cherry 37.5%, northeast mountain cherry 39.3%, cherry anvil king 92.6%.Cherry anvil
The so high sickness rate of king, in can verify that this experimental field, bacterium has spread all over crown gall simultaneously, and what this natural inoculation method was described can
By property.The root knot sickness rate of excellent lobule, below 5%, belongs to high anti-Fructus Pruni pseudocerasi root knot stock；Gisela 6, preferred Folium Isatidis
Leaf, the band ratio of outflow of your this 4 kinds of stocks of Kao Te and Demi 10% about, the stock of anti-Fructus Pruni pseudocerasi root knot in being classified as；GiselaNo.5
Band ratio of outflow 20% about, divides that low anti-root knot stock, great Ye be big, the red tassel of sharp mouth into, cherry, Nanking cherry and northeast mountain cherry
The band ratio of outflow of this five kinds of stocks, in 30%-40%, is susceptible root knot stock.The band ratio of outflow of cherry anvil king is up to 92.6%, for height
Sense root knot stock.The consistent test of design condition, carries out anti-crown gall screening to the cherry rootstock of such multi items, at home
Belong to first.
Table 1：13 kinds of stock band ratio of outflow application forms
3rd, experimental field subsequent treatment
As also needed to carry out the screening of Fructus Pruni pseudocerasi anti-root knot stock, later test, can continue to carry out in this block, the test period is one
Year；
If off-test, do not recycle this block ground, medicine such as copper sulfate, Thiodiazole-copper etc. of test plot killing antibacterial go out antibacterial
Medicament is processed, and interval, after 7-10 days, enriches biological organic fertilizer.
Technical solution of the present invention connects the anti-Fructus Pruni pseudocerasi root knot of screening with Fructus Pruni pseudocerasi Rooted Cuttings and tissue cultured seedling method inoculation Agrobacterium tumefaciems
Stock is compared, and has operation, management simply, can adopt large sample（Each kind repeats 30）, multiple stocks are carried out simultaneously
Test, can quickly, the anti-crown gall of the cherry rootstock of precise Identification selection-breeding.The cherry rootstock filtering out, more can be reflected in field
Resistance, application in being more suitable for producing.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and
Within principle, any modification, equivalent substitution and improvement made etc., should be included within the scope of the present invention.
Claims (4)
1. Fructus Pruni pseudocerasi anti-root knot stock screening technique is it is characterised in that comprise the following steps：
1）, experimental field prepare
In spring the previous year of Fructus Pruni pseudocerasi anti-root knot stock screening, in Fructus Pruni pseudocerasi nursery unit, select, buy band crown gall Fructus Pruni pseudocerasi nursery stock,
Planted in plot, the scale being needed according to Fructus Pruni pseudocerasi anti-root knot stock screening test, determine planting area；
In spring in next year, the band tumor Fructus Pruni pseudocerasi Seedling planted is dug out, soil must not be ploughed deeply；
2）, test implement
The cherry rootstock needing to be tested is carried out cutting lateral root, plants in above-mentioned plot, plant line-spacing and step 1）'s
Line-spacing of planting is identical；
After fall foliage, lifting, clean root system, the sense root knot situation of investigation stock, thus screen anti-root knot cherry rootstock；
3）, experimental field subsequent treatment
As also needed to carry out the screening of Fructus Pruni pseudocerasi anti-root knot stock, later test, can continue to carry out in this block, the test period is one
Year；
If off-test, do not recycle this block ground, Ze Yaoduiquan garden carries out sterilization treatment.
2. according to the anti-root knot stock screening technique of the Fructus Pruni pseudocerasi described in claim 1 it is characterised in that step 1）Experimental field prepare
Described in band root nodule Fructus Pruni pseudocerasi nursery stock according to seeding row spacing（0.2-0.4）m*（0.4-0.6）M is planted.
3. according to the anti-root knot stock screening technique of the Fructus Pruni pseudocerasi described in claim 1 it is characterised in that step 2）Described in plot
In in addition to cultivation experiment cherry rootstock, also plant and have comparison stock, that is, more disease-resistant horse Harry stock and cherry anvil king's stock.
4. according to the anti-root knot stock screening technique of the Fructus Pruni pseudocerasi described in claim 1 it is characterised in that step 3）Described in sterilize
During process, processed using the bacterial agents of going out such as copper sulfate, Thiodiazole-copper, interval, after 7-10 days, enriches biological organic fertilizer.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113115654A (en) * | 2019-12-30 | 2021-07-16 | 西北农林科技大学 | Grape double-stock green branch grafting method |
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2016
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| CN101451112A (en) * | 2008-05-26 | 2009-06-10 | 中国农业大学 | Soil bacilli for preventing and controlling fruit tree crown gall and inocula thereof and preparation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113115654A (en) * | 2019-12-30 | 2021-07-16 | 西北农林科技大学 | Grape double-stock green branch grafting method |
| CN113115654B (en) * | 2019-12-30 | 2023-07-21 | 西北农林科技大学 | Grape double-stock green branch grafting method |
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