CN106467904A - Fusarium graminearum solid medium produces spore method - Google Patents
Fusarium graminearum solid medium produces spore method Download PDFInfo
- Publication number
- CN106467904A CN106467904A CN201510499750.2A CN201510499750A CN106467904A CN 106467904 A CN106467904 A CN 106467904A CN 201510499750 A CN201510499750 A CN 201510499750A CN 106467904 A CN106467904 A CN 106467904A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- wheat stalk
- gibberella saubinetii
- collection
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of fusarium graminearum produces conidial method on solid medium.Can make gibberella saubinetii that conidium is produced on solid medium using the method.
Description
Technical field
The technical problem to be solved in the present invention is to provide a kind of fusarium graminearum to produce conidial method on solid medium, is used for making fusarium graminearum produce conidium on solid medium.
Background technology
Wheat scab is a kind of worldwide disease, generally occurs in each Wheat Production country, especially in the rainy regional serious harm that has a humid climate.China is one of wheat scab occurrence frequency highest and the country by harm most serious, the wheat yield loss that wheat scab causes has had become as one of main loss of wheat yield, cause the underproduction of 10%-15% in the general morbidity time, serious morbidity loss is up to 20%-40%.Wheat scab all can endanger in each period of duration of Semen Tritici aestivi, based on fringe portion harm；DON the toxin remains can be produced in seed, reduce wheat quality during its harm, after eating, person poultry poisoning can be caused.At present, fusarium graminearum is in general culture medium, as PDA culture medium, conidium can't be produced or yield is few, and be commonly used in and make the conidial culture medium of gibberella saubinetii generation be mainly fluid medium, as CMC fluid medium and sweet mung bean soup culture medium, need shaken cultivation 5 days to one week in culture, so the exploitation strengthening fusarium graminearum product spore culture medium is significant to the research of fusarium graminearum other side.
At present, with going deep into that fusarium graminearum is understood, produce spore being many times required for it, to carry out the research of correlation, but the kinds of culture medium being currently used to facilitate fusarium graminearum product spore is also little, type is also more single, so needing to develop the culture medium that a kind of new relatively simple, effect preferably can promote fusarium graminearum to produce spore.
Content of the invention
For solving above-mentioned technical problem, fusarium graminearum cultural method of the present invention is fusarium graminearum to be inoculated into wheat stalk have section position, is cultivated so as to be produced conidium with PDA for carrier.
The present invention application concrete grammar be：The wheat stalk of collection belt segment, prepares PDA culture medium flat board, placement sterilizing belt segment wheat stalk on culture medium flat plate, inoculates fusarium graminearum at wheat stalk belt segment position, and culture is observed under an optical microscope after one week and had or not conidium generation.
The collection of wheat stalk described in present invention application：
Gather wheat stalk in field, straw is cut into the segment of 6-7cm, every section of centre has needed section, after moist heat sterilization, be used for follow-up inoculation.
The preparation of the culture medium described in present invention application：Using conventional PDA culture medium method for production, and 121 DEG C of moist heat sterilization 20min；The flat board of falling PDA culture medium in superclean bench, after culture medium solidifying on every ware flat board place three to four sections of wheat stalks through above-mentioned process, often intersegmental every 1-2cm.
Fusarium graminearum inoculation described in present invention application：In superclean bench, the conidium of fusarium graminearum or hypohostroma are made suspension, draw 10 L inoculation of suspension liquid in the belt segment position of wheat stalk with pipettor, be upside down in culture in 28 DEG C of incubators.
Have the beneficial effects that using produced by technique scheme：The invention provides a kind of fusarium graminearum produces conidial method on solid medium, be applied to make fusarium graminearum that spore to be produced on solid medium, for fusarium graminearum deeper into research provide pathogen material.
Specific embodiment
Collection wheat stalk, is cut into 6-7cm segment and carries out moist heat sterilization；Preparation PDA culture medium flat board, places sterilizing belt segment wheat stalk on culture medium flat plate；Inoculate fusarium graminearum in wheat stalk section portion, culture is observed under an optical microscope after one week and had or not conidium generation.
It is as follows that application the method makes fusarium graminearum produce conidial concrete grammar on solid PDA medium：
1st, the collection of wheat stalk and sterilizing
Gather wheat stalk in field, and straw is cut into the segment of 6-7cm, every section of centre has needed section, after moist heat sterilization, be used for follow-up inoculation；
2nd, the preparation of culture medium
1）Using conventional PDA culture medium manufacture method；
2）Subpackage conical flask, sealing, 121 DEG C of sterilizing 20min；
3）The flat board of falling PDA culture medium in superclean bench；
4）After culture medium solidifying, on every ware flat board place three to four sections of above-mentioned process wheat stalk, often intersegmental every 1-2cm；
3rd, fusarium graminearum inoculation
In superclean bench, the conidium of fusarium graminearum or hypohostroma are made suspension, draw the belt segment position of 10 L inoculation of suspension liquid wheat stalk on culture medium flat plate with pipettor, be upside down in culture in 28 DEG C of incubators.
4th, produce spore situation to observe
After inoculated and cultured one week, in picking culture medium, wheat stalk is had at section, is made interim section with the straw hypohostroma that nearby 1-1.5cm locates in culture medium or beat bacteria cake with card punch and make suspension, optical microphotograph Microscopic observation fusarium graminearum product spore situation no at section straw.
In following embodiments, method therefor is conventional method if no special instructions.
Experimental example：
Gather wheat stalk in field, and straw is cut into the segment of 6-7cm, every section of centre has needed section, after moist heat sterilization, be used for follow-up inoculation；Preparation PDA culture medium, 121 DEG C of moist heat sterilization 20min after coating-dividing sealing；The flat board of falling PDA culture medium in superclean bench；Three to four sections of sterilized belt segment wheat stalks of 6-7cm are put into after culture medium solidifying on every ware flat board, often intersegmental every 1-2cm.
In superclean bench, the conidium of fusarium graminearum or hypohostroma are made suspension, draw the belt segment position of 10 L inoculation of suspension liquid wheat stalk on culture medium flat plate with pipettor, be upside down in culture in 28 DEG C of incubators.
Finally after inoculated and cultured one week, in picking culture medium, wheat stalk is had at section, is no made with the straw hypohostroma that nearby 1-1.5cm locates in culture medium at section straw and cut into slices temporarily, observe fusarium graminearum under an optical microscope and have or not conidium generation, measure sporulation quantity with blood counting chamber method and reach 6.05 × 106Individual/mL.
Embodiment 1：
1st, wheat stalk collection, sterilizing and culture medium preparation.Gather wheat stalk in field, and straw is cut into the segment of about 7cm, every section of centre has needed section, after moist heat sterilization, be used for follow-up inoculation；Preparation PDA culture medium, 121 DEG C of moist heat sterilization 20min after coating-dividing sealing；The flat board of falling PDA culture medium in superclean bench；Three to four sections of sterilized belt segment wheat stalks of 6-7cm are put into after culture medium solidifying on every ware flat board, often intersegmental every 1-2cm.
2nd, fusarium graminearum inoculation.In superclean bench, the conidium of fusarium graminearum or hypohostroma are made suspension, draw the belt segment position of 10 L inoculation of suspension liquid wheat stalk on culture medium flat plate with pipettor, be upside down in culture in 28 DEG C of incubators.
3rd, produce spore situation to observe.After inoculated and cultured one week, in picking culture medium, wheat stalk is had at section, is made interim section with the straw hypohostroma that nearby 1-1.5cm locates in culture medium or beat bacteria cake making suspension with card punch no at section straw, observe fusarium graminearum under an optical microscope and have or not conidium generation, measure sporulation quantity with blood counting chamber method and reach 6.05 × 106Individual/mL.
Claims (5)
1. one kind makes fusarium graminearum produce conidial method on solid medium, is made up of the inoculation of the collection of wheat stalk, the preparation of culture medium and gibberella saubinetii.
2. the collection of the wheat stalk described in claim 1, the preparation of culture medium and gibberella saubinetii be seeded in the application making gibberella saubinetii that spore to be produced on solid medium.
3. the collection of wheat stalk according to claim 2, the preparation of culture medium and gibberella saubinetii be seeded in the application making gibberella saubinetii that spore to be produced on solid medium it is characterised in that：The required wheat stalk of collection fusarium graminearum inoculation moist heat sterilization, preparation PDA culture medium is simultaneously down flat plate, sterilized wheat stalk is put on culture medium flat plate, has section position inoculation gibberella saubinetii in wheat stalk, culture is observed under an optical microscope after one week and had or not generation conidium.
4. the collection of wheat stalk according to claim 3, the preparation of culture medium and gibberella saubinetii be seeded in the application making gibberella saubinetii that spore to be produced on solid medium, it is characterized in that, the inoculation of the described collection of wheat stalk, the preparation of culture medium and gibberella saubinetii：
The collection of wheat stalk：The wheat stalk having section from field collection is used for the inoculation of gibberella saubinetii；
The preparation of culture medium：The preparation of PDA culture medium, weighs 200g peeled potatoes and is cut into small pieces, the 1L that adds water boils 20-30 minute, can be poked by Glass rod, with three layers of filtered through gauze, then plus 15-20g agar, continue heated and stirred to mix, after agar dissolving completely, add 20g glucose, stir, slightly cooling supplies moisture to 1000mL, subpackage conical flask, sealing, 121 DEG C of sterilizing 20min；The flat board of falling PDA culture medium in superclean bench, places three to four sections of 6-7cm after culture medium solidifying on every ware flat board and sterilizes belt segment wheat stalks, often intersegmental every 1-2cm；
Fusarium graminearum is inoculated：In superclean bench, the conidium of gibberella saubinetii or hypohostroma are made suspension, draw the belt segment position of 10 L inoculation of suspension liquid wheat stalk on culture medium flat plate with pipettor, be upside down in culture in 28 DEG C of incubators.
5. the collection of the wheat stalk according to claim 3 or 4, the preparation of culture medium and gibberella saubinetii the application making gibberella saubinetii produce in spore is seeded on solid medium, it is characterized in that, the described wheat stalk gathering belt segment first, then prepare PDA culture medium and be down flat plate, sterilized belt segment wheat stalk is put on culture medium flat plate, finally inoculate gibberella saubinetii at wheat stalk belt segment position, observe under an optical microscope after culture a period of time and have or not conidium generation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510499750.2A CN106467904A (en) | 2015-08-15 | 2015-08-15 | Fusarium graminearum solid medium produces spore method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510499750.2A CN106467904A (en) | 2015-08-15 | 2015-08-15 | Fusarium graminearum solid medium produces spore method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106467904A true CN106467904A (en) | 2017-03-01 |
Family
ID=58213554
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510499750.2A Pending CN106467904A (en) | 2015-08-15 | 2015-08-15 | Fusarium graminearum solid medium produces spore method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106467904A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113234604A (en) * | 2021-04-30 | 2021-08-10 | 裕菁科技(上海)有限公司 | Preparation method of mixed culture medium for mycotoxin production |
| CN116622614A (en) * | 2023-02-06 | 2023-08-22 | 中国科学院南京土壤研究所 | Preparation method of fusarium graminearum spore liquid |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011016921A2 (en) * | 2009-07-29 | 2011-02-10 | The United States Of America, As Represented By The Secretary Of Agriculture | Prothioconazole tolerant cryptococcus flavescens strains for biological control of fusarium head blight |
| CN102123596A (en) * | 2007-12-14 | 2011-07-13 | 农业研究基金会 | Novel micro-organisms controlling plant pathogens |
| CN104087515A (en) * | 2014-06-30 | 2014-10-08 | 河南科技大学 | Method for separating wheat stem base rot bacteria |
-
2015
- 2015-08-15 CN CN201510499750.2A patent/CN106467904A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102123596A (en) * | 2007-12-14 | 2011-07-13 | 农业研究基金会 | Novel micro-organisms controlling plant pathogens |
| WO2011016921A2 (en) * | 2009-07-29 | 2011-02-10 | The United States Of America, As Represented By The Secretary Of Agriculture | Prothioconazole tolerant cryptococcus flavescens strains for biological control of fusarium head blight |
| CN104087515A (en) * | 2014-06-30 | 2014-10-08 | 河南科技大学 | Method for separating wheat stem base rot bacteria |
Non-Patent Citations (1)
| Title |
|---|
| 张向向等: "我国冬小麦主产省小麦茎基腐镰孢菌的组成及其致病力", 《麦类作物学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113234604A (en) * | 2021-04-30 | 2021-08-10 | 裕菁科技(上海)有限公司 | Preparation method of mixed culture medium for mycotoxin production |
| CN116622614A (en) * | 2023-02-06 | 2023-08-22 | 中国科学院南京土壤研究所 | Preparation method of fusarium graminearum spore liquid |
| CN116622614B (en) * | 2023-02-06 | 2024-05-28 | 中国科学院南京土壤研究所 | Preparation method of fusarium graminearum spore liquid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107446847A (en) | One plant of Bei Laisi bacillus GT11 and its application | |
| CN103330258A (en) | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof | |
| CN103451135A (en) | Bacillus subtilis M3 and application thereof | |
| CN103756916B (en) | A kind of Acremonium terricola mutant strain and application thereof | |
| CN103571756B (en) | Test tube screening method of isaria cicadae strain and culture medium | |
| CN105586274A (en) | Trichoderma koningiopsis T-51 strain and application thereof in growth promotion of tomatoes and biological prevention and control of Botrytis cinerea Pers | |
| CN103190279A (en) | Submerged fermentation of Armillaria luteovirens and application thereof | |
| CN107432135A (en) | Promote the method for cynomorium songaricum seed sprouting using fungi | |
| CN102876767A (en) | Method for screening alternaria alternata resistant germplasm material from petri dish | |
| CN106467904A (en) | Fusarium graminearum solid medium produces spore method | |
| CN104403952B (en) | A kind of sclerotium oyster mushroom novel bacterial and its cultural method and application | |
| CN108293593B (en) | Method for cultivating black fungus by using corncob mixture | |
| CN108342429A (en) | A kind of preparation method of tremella spore fermentation high yield tremella polysaccharides | |
| CN106719645B (en) | A kind of botanical fungicide and preparation method thereof for preventing and treating dry rot of potato | |
| CN104945129A (en) | Mushroom culture medium | |
| CN109628369A (en) | A kind of millet blast bacterium product spore culture medium and preparation method thereof | |
| CN110699352A (en) | Breeding method of trichoderma harzianum high-yield strain for preventing and controlling potato root rot | |
| CN105296416B (en) | The method that Rumex gmelini Turcz suspension cell is established with its endogenetic fungus co-culture system | |
| CN109762745A (en) | A kind of fermentation culture method of Pleurotus tuber-regium and the method that active constituent is extracted from culture | |
| CN109906872A (en) | A kind of lucidum strain production method | |
| CN115039633B (en) | Artificial culture method for sporophore of Isaria japonica | |
| CN105586267B (en) | Produce the ganoderma lucidum mutagenic strain of ganoderma lucidum mycelium | |
| CN103864499A (en) | Mushroom 808 liquid strain cultivation medium, manufacturing method and mushroom 808 cultivation method | |
| CN107156297A (en) | A kind of purple sesame Yoghourt and preparation method thereof | |
| CN112514736A (en) | Method for cultivating lentinus edodes by using stevia rebaudiana leaf residues |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170301 |
|
| RJ01 | Rejection of invention patent application after publication |