CN106459160A - Asx特异性蛋白质连接酶 - Google Patents

Asx特异性蛋白质连接酶 Download PDF

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CN106459160A
CN106459160A CN201580022063.XA CN201580022063A CN106459160A CN 106459160 A CN106459160 A CN 106459160A CN 201580022063 A CN201580022063 A CN 201580022063A CN 106459160 A CN106459160 A CN 106459160A
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基恩·特鲁茨·江·阮
谈秉军
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Abstract

本发明针对具有Asx特异性连接酶和环化酶活性的酶，并且针对编码那些酶的核酸以及所述酶的生产方法。进一步涵盖的是这些酶的方法和用途。

Description

ASX特异性蛋白质连接酶
相关申请的交叉引用
本申请要求于2014年4月24日提交的美国临时专利申请号61/983,729的优先权权益，出于所有目的将该临时申请的内容通过引用以其全部内容并入本文。
技术领城
本发明属于酶技术的技术领域，并且具体地涉及新颖的具有Asx特异性连接酶和环化酶活性的酶，并且涉及编码那些酶的核酸以及生产所述酶的方法。进一步涵盖的是这些酶的方法和用途。
背景技术
肽和蛋白质的头至尾大环化已经被用作约束结构和增强抗蛋白水解降解的代谢稳定性的策略。另外，受约束的大环构象还可以改进药理活性和口服生物利用度。尽管大部分肽和蛋白质作为直链被产生，但是在多种多样的生物体中仍天然地存在范围从6至78个残基的环肽。这些环肽通常展示出对热变性和蛋白水解的高度抗性，并且已经在蛋白质工程中激发了新趋势，如通过在细胞因子、组胺素(histatin)、泛素C-末端水解酶、芋螺毒素和缓激肽接枝的大环寡肽(cyclotide)的环化中的近期成功所证明的。此外，环肽已经被用作治疗剂，包括缬氨霉素、短杆菌肽S和环孢霉素。
迄今为止，化学方法被典型地用于肽的环化。一种可能的策略是天然化学连接(native chemical litigation)。这种方法需要N-末端半胱氨酸和C-末端硫酯，这些要求限制了所述方法对于不含半胱氨酸的肽的应用。此外，化学方法并非总是可行的，尤其是对于大的肽和蛋白质。
尽管利用天然存在的环化酶的酶法将是理想的，但是当前仅已知非常少的肽环化酶并且由于各种原因它们未被充分利用。然而，其他酶(如其固有功能并非环化酶的分选酶A和内含肽)已经被成功地应用于多种肽和蛋白质的环化。尽管如此，但是这些酶具有缺点。例如，分选酶A是一种将表面蛋白锚定到细菌细胞壁的转肽酶。它的环化反应通常需要过夜孵育和0.1至1摩尔当量的酶。此外，分选酶A具有五肽识别序列LPXTG，并且在被修饰的蛋白质上留下不必要的标签。内含肽是已经用于大环寡肽、向日葵胰蛋白酶抑制剂、和q-防御素的表达的自催化剪接元件。然而，内含肽介导的环化需要靶蛋白与内含肽结构域的基因融合，该必要条件可以影响蛋白质折叠或溶解性。
因此在本领域仍需要用于使肽和蛋白质环化的、克服现有技术的缺陷并且理想地是简单、快速和通用的改进手段。
发明内容
本发明通过提供新颖的满足以上要求的Asx特异性蛋白质连接酶满足了这个需要。诸位发明人已经出人意料地发现，从药用植物蝶豆(Clitoria ternatea)中分离出的这种酶是一种天然存在的环化酶，其在大环寡肽(一个大的植物环肽家族)的合成中被用作加工酶。已经发现这种酶是到目前为止最快的已知连接酶，其催化活性高达542,000M-1s-1。它识别C-末端处的三肽基序Asx-His-Val，并且通过切割分选序列His-Val并将Asx连接到N-末端残基上以形成环形拓扑结构而介导肽主链环化。可以显示所述酶不仅有效地环化大环寡肽前体和大小范围从14至58个残基的多种富半胱氨酸肽，并且还有效地环化不含半胱氨酸的肽和绿色荧光蛋白(GFP)。这使得它在希望使给定肽或蛋白质环化的各种应用中是高度通用且有用的。
在第一方面中，本发明因此涉及包含如在SEQ ID NO:1中列出的氨基酸序列或由其组成的分离的多肽。由在SEQ ID NO:1中列出的氨基酸序列组成的多肽在本文也被称为“butelase 1”。
在另一个方面中，本发明还涉及编码在本文描述的多肽的核酸分子，以及含有这样的核酸的载体，特别是拷贝载体或表达载体。
在再一个方面中，本发明还针对含有如本文所考虑的核酸或如本文所考虑的载体的宿主细胞，优选非人类宿主细胞。
本发明的仍再一个方面是用于生产本文所述的多肽的方法，所述方法包括培养本文所考虑的宿主细胞；并且从培养基或从宿主细胞中分离多肽。
在仍再一个方面中，本发明涉及在本文描述的多肽用于蛋白质连接，特别是用于环化一种或多种肽的用途。
本发明的另一个方面针对以下多肽用于连接至少两种肽或使肽环化的用途，所述多肽包含以下项或由其组成：
(i)如在SEQ ID No:3-109中列出的任一个氨基酸序列；
(ii)在其整个长度上与(i)的任一个氨基酸序列具有至少60％、优选至少70％、更优选至少80％、最优选至少90％序列同一性的氨基酸序列；
(iii)在其整个长度上与(i)的任一个氨基酸序列具有至少80％、优选至少90％、更优选至少95％序列同源性的氨基酸序列；或
(iv)(i)-(iii)中任一项的片段。
在仍另一个方面中，本发明涉及用于使肽环化的方法，该方法包括结合本发明的用途，在允许环化所述肽的条件下用上文描述的多肽孵育所述肽。
在仍再一个方面中，本发明涉及用于连接至少两种肽的方法，该方法包括结合本发明的用途，在允许连接所述肽的条件下用上文描述的多肽孵育所述肽。
在另一个方面中，本发明涉及本发明的分离的多肽被固定到其上的固体支持材料及其用途和使用此类底物的方法。
在另一个方面中，本发明还涵盖包含编码本文所述的具有蛋白质连接酶和/或环化酶活性的多肽的核酸分子的转基因植物。多肽优选地不天然地存在于所述植物中。因此，本发明还重点包括表达根据本发明的异源多肽的转基因植物。
附图说明
图1示出了kB1-NHV的氧化折叠。使所述肽在含有50％乙腈、100mM碳酸氢铵、3mM还原型谷胱甘肽的缓冲液(pH 8.0)中在30μM浓度下折叠18h。折叠的肽最后在RP-HPLC中洗脱。
图2示出了肽环化酶活性的MS表征。(a)由butelase 1介导的kB1-NHV环化的示意图。对butelase 1识别位点处的残基(P1、P1'和P2’)进行了标记。(b,c)分别由蝶豆(C.Ternatea)粗提物和纯化的butelase 1介导的kB1-NHV环化的MS曲线。括号中的肽是cliotide，蝶豆中天然存在的大环寡肽。产物环状kB1由箭头指示。(d)刀豆豆荚蛋白(Jackbean legumain)被用作对照。MS曲线显示刀豆豆荚蛋白水解kB1-NHV中的天冬酰胺酰基键，以给出线性形式的kB1。用K+或K2 +标记的峰分别是对应于一个或两个钾离子的结合的离子加合物。
图3示出了酶环化的kB1和天然肽的共洗脱。(a)酶环化的kB1的HPLC曲线。(b)从O.affinis中提取的天然kB1的HPLC曲线。(c)酶环化的和天然的kB1的共洗脱曲线。
图4示出了从butelase 1对kB1-NHV的转化获得的酶环化的kB1中的环状主链的MS证据。(a)S-脲甲基化之后环化的kB1的MS曲线。环化的kB1具有2891的m/z值，所述值在S-烷基化之后变为3239。在3182处观察到不完全烷基化造成的次峰，其中仅有5个半胱氨酸被修饰。(b)胰蛋白酶消化之后S-烷基化的kB1的MS曲线。观察到18Da的质量增加，这指示水分子和环状主链的加入。(c)3257-Da胰蛋白酶片段的MS/MS曲线。肽序列示出在MS/MS谱的顶端。y-离子被标记在对应峰的顶端。
图5示出了酶环化的kB1(深灰色)和天然kB1(浅灰色)的1D NMR谱比较。将肽溶解于pH 4.3的95％H2O/5％D2O中。在298K下记录谱。
图6示出了butelase 1的分离、表征和同源建模。(a)纯化的butelase 1的SDS-PAGE分析。通过银染将蛋白质可视化。左泳道是纯化的butelase 1，并且右泳道是具有指示的分子量(kDa)的蛋白梯。(b)豆荚蛋白特异性探针LP-1对butelase 1的标记。将蛋白质通过SDS-PAGE分辨，并且通过具有Cy5过滤器的Typhoon扫描仪(GE Health Care)可视化。(c)从EST序列推导的butelase 1前体的翻译序列。所述序列是彩色编码的，其中内质网信号以黑色示出，N-末端前域(prodomain)以橙色示出，AEP结构域以蓝色示出，活性肽区域以品红色示出，并且LSAM结构域以灰色示出。对纯化的活性肽的第一个和最后一个残基(V42和N383)进行了标记。催化三联体(Asn59、His165和Cys207)的保守残基是斜体的。从胶内胰蛋白酶消化获得的肽序列是加下划线的。(d)基于人类豆荚蛋白结构的酶原butelase 1的建模结构。左图示出了建模的酶原butelase 1，其中AEP结构域以蓝色示出，活性肽区域以品红色示出并且LSAM结构域以灰色示出。右上图示出了建模的butelase 1和模板人类豆荚蛋白(PDB ID:4FGU；黄色)的结构比对。催化三联体残基(Asn59、His165和Cys207)在黑色虚线框内，以及在右下图中的放大图内以红色棒高亮。
图7示出了胶内胰蛋白酶消化对butelase 1的蛋白质鉴定。通过MS/MS对五个优势胰蛋白酶片段进行测序并且示出在MS曲线的顶端。
图8示出了豆荚蛋白特异性探针LP-1的化学结构。
图9示出了作为肽环化酶的butelase 1的动力学表征。(a)在45min的时间过程中环化反应的RP-HPLC踪迹。对底物kB1-NHV和产物kB1进行了标记。在0.125μM butelase1和50μM kB1-NHV的存在下在37℃下进行试验。在220nm的波长下监测吸光度。(b-d)针对kB1-NHV、SFTI-NHV和SA-kB1-NHV的butelase 1动力学的Michaelis-Menten图。通过将产物的HPLC-峰面积转化为浓度来计算环化速率。为了对kB1-NHV和SFTI-NHV进行动力学测量，使试验在0.125μM butelase 1和变化的底物浓度的存在下在37℃下进行12min。对于SA-kB1-NHV，归因于快得多的环化速率，使用5nM的酶浓度代替0.125μM并且将孵育时间减少到6min。
图10示出了kB1的环二聚体形成。(a)RP-HPLC曲线图示了kB1的环二聚体形成。使反应在0.125μM butelase 1和500μM kB1-NHV的存在下在37℃下进行4h。峰1是与天然环状kB1具有为2891的相同m/z值的kB1异构体。峰2具有2893的m/z值，这指示减少了kB1的一个二硫键。在峰顶点处对底物kB1-NHV和产物kB1和(kB1)2进行了标记。(b)MS曲线图示了kB1的环二聚体形成。用K+标记的峰是钾加合物。
图11示出了用butelase 1处理之后kB1-NHV截短的类似物的MS曲线。使试验在0.125μM butelase 1和50μM的每种底物的存在下在37℃下进行30h。(a)用butelase 1处理的kB1-NH的MS曲线。(b)用butelase 1处理的kB1-N*的MS曲线。在峰顶点处对kB1产物和截短的类似物kB1-NH和kB1-N*进行标记。m/z值为2969.4的峰表示修饰的环状kB1，其中一个半胱氨酸残基用β-Me来S-烷基化。用K+和K2 +标记的峰分别是对应于一个或两个钾离子的结合的离子加合物。
图12示出了用butelase 1处理之后kB1-NHV类似物的MS曲线。(a-d)P1位置处的保守Asn被kB1-NHV底物中的Glu、Gln、Ala或Asp残基置换。使试验在0.125μM butelase 1和50μM的每种底物的存在下在37℃下进行4h。在峰顶点处对kB1-NHV类似物的质量进行了标记。在kB1-DHV的情况下，观察到环化产物的m/z值为2892。用K+和K2 +标记的峰分别是对应于一个或两个钾离子的结合的离子加合物。
图13示出了用刀豆豆荚蛋白和butelase 1处理的Z-AAN-AMC的HPLC曲线。(a)作为对照的Z-AAN-AMC的HPLC踪迹。(b)示出被刀豆豆荚蛋白催化的的水解产物7-氨基-4-甲基香豆素的HPLC踪迹。使试验在8μU刀豆豆荚蛋白和50μM Z-AAN-AMC的存在下在37℃下进行30h。(c)示出butelase 1对Z-AAN-AMC的作用的HPLC踪迹。使试验在0.125μM butelase 1和50μM Z-AAN-AMC的存在下在37℃下进行30h。在30h孵育之后未观察到明显的水解产物(由箭头指示)。在254nm的波长下监测吸光度。
图14示出了用GIGGIR(SEQ ID NO:123)进行的SA-pyroGlu-kB1-NHV连接的HPLC曲线。(a)作为对照的SA-pyroGlu-kB1-NHV的HPLC踪迹。(b)示出不存在butelase1的情况下水解产物SA-pyroGlu-kB1-N的HPLC踪迹。(c)示出在5倍过量的GIGGIR(250μM)的存在下连接反应的HPLC踪迹。(d)示出在20倍过量的GIGGIR(1mM)的存在下连接反应的HPLC踪迹。使连接反应在0.125μM butelase 1和50μM SA-pyroGlu-kB1-NHV及变化浓度的GIGGIR(0-1mM)的存在下在37℃下进行20min。
图15示出了butelase介导的肽连接的受体特异性。(a)通过butelase 1促进的KALVINHV(SEQ ID NO:122)和XIGGIR(SEQ ID NO:123)的分子间连接。使反应在100nMbutelase 1、100μM KALVINHV和1mM XIGGIR的存在下进行并孵育10min或2h。通过将HPLC峰面积转化为浓度来计算连接产率。(b)通过butelase 1促进的KALVINHV和LXGGIR的分子间连接。使反应在100nM butelase 1、100μM KALVINHV和1mM LXGGIR的存在下进行并孵育10min或2h。通过将HPLC峰面积转化为浓度来计算连接产率。
图16示出了butelase介导的人类神经介肽U、心血管调节肽a、apelin和甘丙肽以及大鼠神经介肽U的环化。使环化反应在含有50μM肽和0.1μM butelase 1(0.002摩尔当量)的50μl反应混合物中在37℃下进行并且通过MS进行监测。
图17示出了butelase介导的在C-末端以Asn-His-Val基序结束并且在N-末端以Gly-Ile开始的修饰的GFP的环化。使环化反应在25μM GFP和0.1μM butelase 1(0.004摩尔当量)的存在下进行。通过高分辨率ESI-MS来监测环化反应。
图18示出了butelase介导的GFP与短肽GIGK(生物素)R(SEQ ID NO:134)的连接。使连接反应在0.125μM butelase 1、50μM GFP和1mM肽底物的存在下在37℃下进行30分钟并且通过MS进行监测。
图19示出了荧光标记的肽GIR-AMC(AMC＝7-氨基-4-甲基香豆素)与(a)ABL-Mono和(b)ERK-Darp之间孵育20分钟的连接的质谱曲线。7232和7354.8是ABL-Mono及其被质子化一次的连接产物的峰。10251.4和6031.3是质子化一次和两次的ERK-Darp连接产物的峰。
图20示出了下述的ESI-MS曲线：(a,c)作为阴性对照的ERK-Darp和GFP-NHV底物，(b,d)用1mM FITC-GKNHV和50nM butelase 1在42℃下孵育10分钟的50μM ERK-Darp和50μMGFP-NHV。
图21示出了泛素(SEQ ID NO:148)和肽YKN-巯基乙酸-V之间的连接的HPLC和MS曲线。
图22示出了butelase 1介导的二聚化的分析。(a)G2K二聚体核心肽的结构，(b)和(c)两种不同的可能的单连接的G2K肽的结构，(d)完全连接的二聚体肽的结构。(e)二聚化反应的质谱分析示出了单连接的和完全连接的二聚体的存在。
图23示出了butelase介导的肽环化的受体特异性。使反应在50nM butelase 1、50μM肽的存在下进行并在42℃下孵育60min。通过将HPLC峰面积转化为浓度来计算环化产率。(a)通过butelase 1促进的XLYRRGRYLRRNHV(SEQ ID NO:157)的分子内环化。(b)通过butelase 1促进的XRLYRGRYLRRNHV(SEQ ID NO:158)的分子内连接。(c)通过butelase 1促进的GXLYRGRYLRRNHV(SEQ ID NO:159)的分子内连接。
图24示出了通过固定在ProSwift ConA-1S亲和柱上的butelase 1进行的肽底物芋螺毒素、SFTI和kalata B1的环化试验。
具体实施方式
本发明基于诸位发明人鉴定了从蝶豆中分离的肽连接酶/环化酶，所述酶能够以非常高的催化活性连接/环化肽。它识别C-末端处的三肽基序Asx-His-Val，并且通过切割分选序列His-Val并将Asx连接到N-末端残基上以形成环形拓扑结构而介导肽主链环化。值得注意地，所述酶不仅可以有效地环化大环寡肽前体和大小范围从14至58个残基的多种富半胱氨酸肽，并且还可以有效地环化不含半胱氨酸的肽和蛋白质(如绿色荧光蛋白(GFP))。这使得它在期望环化给定肽或蛋白质的各种应用中是高度通用且有用的。
在第一方面中，本发明包括处于分离形式的所述酶，并且更具体地针对包含如在SEQ ID NO:1中列出的氨基酸序列、基本上由其组成或由其组成的分离的多肽。由在SEQ IDNO:1中列出的氨基酸序列组成的多肽在本文也被称为“butelase 1”。如在本文使用的，“分离的”涉及处于下述形式的多肽，其中所述多肽已经至少部分地与其可以天然地存在或相关的其他细胞组分分离。所述多肽可以是重组多肽，即产生于不天然地产生所述多肽的基因工程化的生物体中的多肽。
根据本发明的多肽展现出蛋白质连接活性，即它能够在两个氨基酸残基之间形成肽键，其中这两个氨基酸残基位于相同或不同的肽或蛋白质上，优选地位于相同的肽或蛋白质上，这样使得所述连接活性环化所述肽或蛋白质。因此，在不同实施方案中，本发明的多肽具有环化酶活性。在不同实施方案中，这种蛋白质连接或环化酶活性还包括内肽酶活性，即所述多肽在两个氨基酸残基之间形成肽键并且同时切割现有的肽键。这意味着环化无需发生在给定肽的末端之间，而是也可以发生在内部氨基酸残基之间，其中在用于环化的氨基酸的C-末端或N-末端的氨基酸被切割掉。在优选实施方案中，多肽通过将N-末端连接到内部氨基酸上并切割剩余的C-末端氨基酸而形成环化的肽。
如在本文披露的多肽是“Asx特异性的”，因为发生连接的氨基酸C-末端(即所连接的肽的C-末端)是天冬酰胺(Asn或N)或天冬氨酸(Asp或D)，优选是天冬酰胺。在不同实施方案中，根据本发明的多肽还具有针对下述肽的连接活性，所述肽具有酰胺化的C-末端Asx(N或D)残基，即C-末端羧基基团被酰胺基置换。这个酰胺基在连接反应的过程中被切割掉。因此，此类酰胺化的肽底物，虽然仍被连接/环化，但不包含天然地存在的三肽基序NHV。
如在本文使用的，“多肽”涉及通过肽键连接的由氨基酸构成的聚合体。如在本文定义的，多肽可以包含50个或更多个氨基酸，优选100个或更多个氨基酸。如在本文使用的，“肽”涉及通过肽键连接的由氨基酸构成的聚合体。如在本文定义的，肽可以包含2个或更多个氨基酸，优选5个或更多个氨基酸，更优选10个或更多个氨基酸，例如10至50个氨基酸。
在不同实施方案中，所述多肽包含以下氨基酸序列或由其组成，所述氨基酸序列在其整个长度上与在SEQ ID NO:1中列出的氨基酸序列至少60％、65％、70％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、90.5％、91％、91.5％、92％、92.5％、93％、93.5％、94％、94.5％、95％、95.5％、96％、96.5％、97％、97.5％、98％、98.5％、99％、99.25％、或99.5％相同或同源。在一些实施方案中，它具有在其整个长度上与在SEQ ID NO:1中列出的氨基酸序列具有至少60％、优选至少70％、更优选至少80％、最优选至少90％序列同一性的氨基酸序列，或者具有在其整个长度上与在SEQID NO:1中列出的氨基酸序列具有至少80％、优选至少90％、更优选至少95％序列同源性的氨基酸序列。
在不同实施方案中，所述多肽可以是成熟酶的前体。在此类实施方案中，它可以包含在SEQ ID NO:2中列出的氨基酸序列或由其组成。还涵盖的是具有以下氨基酸序列的多肽，所述氨基酸序列在其整个长度上与在SEQ ID NO:2中列出的氨基酸序列至少75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、90.5％、91％、91.5％、92％、92.5％、93％、93.5％、94％、94.5％、95％、95.5％、96％、96.5％、97％、97.5％、98％、98.5％、99％、99.25％、或99.5％相同或同源。
通常借助序列比较确定核酸序列或氨基酸序列的同一性。这种序列比较是基于在现有技术中建立的且常用的BLAST算法(参见例如Altschul等人(1990)“Basic localalignment search tool”，J.Mol.Biol.215：403-410，和Altschul等人(1997)：“GappedBLAST and PSI-BLAST:a new generation of protein database search programs”；Nucleic Acids Res.，25，第3389-3402页)，并且原则上通过分别将核酸序列和氨基酸序列中相似的连续核苷酸或氨基酸相互关联来实现。相关位置的列表关联称为“比对”。序列比较(比对)特别是多序列比较常见地使用对本领域普通技术人员而言可获得且已知的计算机程序进行准备。
这种比较也允许阐明被比较的序列之间的相似性。这通常被表示为百分比同一性，即在比对中在相同位置处或在彼此相对应的位置处的相同核苷酸或氨基酸残基的比例。在氨基酸序列的背景下，更广义解释的术语“同源性”还考虑了保守氨基酸替换，即具有相似化学活性的氨基酸，因为这些氨基酸通常在蛋白质内发挥相似的化学活性。所比较序列的相似性也可以因此被表示为“百分比同源性”或“百分比相似性”。同一性和/或同源性的表示可以针对整个多肽或基因，或者仅针对个别区域。各个核酸序列或氨基酸序列的同源和相同区域因此通过序列中匹配的方式来定义。此类区域通常展现出相同的功能。它们可以较小，并且可以仅涵盖几个核苷酸或氨基酸。这种小区域通常发挥对蛋白质的总体活性而言必要的功能。因此仅针对个别(并且任选地小)区域提及序列匹配可以是有用的。然而，除非另外指示，否则在本文同一性和同源性的表示是指对应地指示的核酸序列或氨基酸序列的全长。
在不同实施方案中，在本文描述的多肽在对应于SEQ ID NO:1的位置19的位置处包含氨基酸残基N；和/或在对应于SEQ ID NO:1的位置124的位置处包含氨基酸残基H；和/或在对应于SEQ ID NO:1的位置166的位置处包含氨基酸残基C。已经发现这些氨基酸残基推定地在所述多肽的催化活性中发挥作用。在优选实施方案中，多肽因此在给定的或对应的位置处包含至少两个、更优选所有三个上文指示的残基。
本发明的分离的多肽优选地具有酶活性，特别是蛋白质连接酶，优选环化酶活性。在不同实施方案中，这意味着它们能以80％或更高、优选90％或更高的效率连接给定肽。蛋白质连接(优选环化)反应优选地是比较快的，即所述多肽能以500μM或更小、优选250μM或更小的Km；和/或至少0.05s-1、优选至少0.5s-1、更优选至少1.0s-1、最优选至少1.5s-1的kcat环化给定肽。优选的多肽满足两个要求，即Km和kcat要求。用于确定此类Michaelis-Menten动力学的方法在本领域是众所周知的并且可以常规地由本领域普通技术人员来应用。优选的是本发明的多肽具有以下酶的至少50％、更优选至少70％、最优选至少90％的蛋白质连接酶活性，所述酶具有SEQ ID NO:1的氨基酸序列。
根据在本文描述的实施方案的多肽可以包含氨基酸修饰，特别是氨基酸取代、插入、或缺失。此类多肽例如通过靶向遗传修饰(即通过诱变方法的方式)进行进一步开发，并且针对具体目的或相对于特殊特性(例如，相对于其催化活性、稳定性等)进行优化。另外，本文所考虑的核酸可以引入重组制剂中并且由此用于产生完全新颖的蛋白质连接酶、环化酶或其他多肽。
在不同实施方案中，具有连接酶/环化酶活性的多肽可以是翻译后修饰的，例如糖基化的。此类修饰可以通过重组手段进行，即在产生后直接在宿主细胞中进行，或者可以在(例如在体外)合成多肽之后化学地或酶促地实现。
目的可以在于将靶向突变(如取代、插入、或缺失)引入已知分子中，以便例如改变底物特异性和/或改进催化活性。为此目的，具体而言，可以修饰分子的表面电荷和/或等电点，并且由此修饰它们与底物的相互作用。可替代地或另外地，可以通过一个或多个对应的突变的方式增强多肽的稳定性，并且由此改进其催化性能。单独突变(例如单独取代)的有利特性可以彼此补充。
在不同实施方案中，所述多肽的特征可以在于它是可通过单个或多个保守性氨基酸取代从如上文描述的多肽作为初始分子而获得的。术语“保守性氨基酸取代”意指将一个氨基酸残基替换(取代)为另一个氨基酸残基，其中这样的替换不会导致交换的氨基酸位置处发生极性或电荷改变，例如将非极性氨基酸残基替换为另一个非极性氨基酸残基。在本发明的背景下，保守性氨基酸取代涵盖例如G＝A＝S、I＝V＝L＝M、D＝E、N＝Q、K＝R、Y＝F、S＝T、G＝A＝I＝V＝L＝M＝Y＝F＝W＝P＝S＝T。
可替代地或另外地，所述多肽的特征可以在于它是可通过片段化或通过缺失、插入、或取代诱变从本文考虑的多肽作为初始分子而获得的，并且涵盖在至少150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、300、310、320、325、330、331、332、333、334、335、336、337、338、339、340、341、或342个连续连接的氨基酸的长度上匹配所述初始分子的氨基酸序列。优选的是在此类实施方案中，包含在初始分子中的氨基酸N19、H124和C166仍存在。
在不同实施方案中，本发明因此还涉及本文描述的多肽的片段，其中所述片段保留酶活性。优选的是它们具有初始分子的、优选地具有SEQ ID NO:1的氨基酸序列的多肽的至少50％、更优选至少70％、最优选至少90％的蛋白质连接酶和/或环化酶活性。所述片段的长度优选地是至少150个、更优选地至少200或250个，最优选地至少300个氨基酸。进一步优选的是这些片段在对应于SEQ ID NO:1的位置19、124和166的位置处包含氨基酸N、H和C。优选的片段因此包含在SEQ ID NO:1中列出的氨基酸序列的氨基酸19-166，更优选地10-200，最优选地1-277。
编码在本文描述的多肽的核酸分子，以及含有这样的核酸的载体，特别是拷贝载体或表达载体，也形成本发明的一部分。
这些可以是DNA分子或RNA分子。它们可以作为单链、作为与所述单链互补的单链、或作为双链存在。特别是对于DNA分子，在每种情况下都应当以所有三个可能的阅读框来考虑两条互补链的序列。还应当考虑的是以下事实，即不同的密码子(即碱基三联体)可以编码相同的氨基酸，这样使得特定的氨基酸序列可以由多个不同的核酸来编码。作为这种遗传密码简并性的结果，可以编码上文描述的多肽之一的所有核酸序列都被包括在本发明的主题中。本领域技术人员能够明确地确定这些核酸序列，因为尽管存在遗传密码的简并性，但是所限定的氨基酸会与个别密码子相关联。本领域技术人员可以因此从氨基酸序列着手而容易地确定编码所述氨基酸序列的核酸。另外，在根据本发明的核酸的背景下，一个或多个密码子可以被同义密码子置换。这个方面特别是指本文所考虑的酶的异源表达。例如，每个生物体(例如生产株的宿主细胞)都具有特定的密码子选择。“密码子选择”被理解为由对应的生物体将遗传密码翻译成氨基酸。如果位于核酸上的密码子遇到了在生物体中相对小数目的装载的tRNA分子，则可以发生蛋白质生物合成的瓶颈。其仍编码相同的氨基酸，但结果是密码子在生物体中的翻译效率低于编码相同氨基酸的同义密码子。由于存在较大数目的用于同义密码子的tRNA分子，后者可以在生物体中更有效地被翻译。
通过目前通常已知的方法，例如像化学合成或聚合酶链式反应(PCR)与分子生物学或蛋白质化学的标准方法组合，本领域技术人员有能力在已知的DN序列和/或氨基酸序列的基础上生产出对应的核酸直至完整的基因。此类方法例如从Sambrook，J.，Fritsch，E.F.，和Maniatis，T，2001，Molecular cloning：a laboratory manual，第3版，ColdSpring Laboratory Press已知。
出于本文的目的，“载体”被理解为由核酸构成的元件，其含有本文考虑的核酸作为表征核酸区域。它们使得所述核酸能够被建立为在物种或细胞系中历经多个世代或细胞分化的稳定遗传元件。特别是当在细菌中使用时，载体是特定的质粒(即环状遗传元件)。在本文的背景下，如本文考虑的核酸被克隆到载体中。所述载体包括例如其来源为细菌质粒、病毒、或噬菌体的那些，或者具有广泛不同来源的元件的、主要为合成的载体或质粒。使用在每种情况下存在的另外的遗传元件，载体能够将其本身建立为相关宿主细胞中历经多个世代的稳定单元。它们可以作为分离的单元存在于染色体外，或者可以整合到染色体中，或分别整合到染色体DNA中。
表达载体涵盖下述核酸序列，其能够在含有它们的宿主细胞、优选微生物、特别优选细菌中复制并在其中表达所含的核酸。在不同实施方案中，在本文描述的载体因此还含有控制编码本发明多肽的核酸的表达的调节元件。表达特别受调节转录的一个或多个启动子的影响。原则上可以借助原本位于待表达的核酸之前的天然启动子来进行表达，但是也可以借助配备于表达载体上的宿主细胞启动子来进行表达，或者也可以借助另一个生物体的或另一个宿主细胞的修饰的、或完全不同的载体来进行表达。在本发明的情况下，使至少一种用于表达本文所考虑的核酸的启动子可用，并用于其表达。可例如通过改变培养条件的方式，或者当含有表达载体的宿主细胞达到特定的细胞密度时，或者通过加入特定物质，特别是基因表达的激活剂，来进一步调解表达载体。这样的物质的一个实例是半乳糖衍生物异丙基-β-D-硫代半乳糖吡喃糖苷(IPTG)，其被用作细菌乳糖操纵子(lac操纵子)的激活剂。与表达载体不同，所含的核酸在克隆载体中不表达。
在再一个方面中，本发明还针对含有如本文所考虑的核酸或如本文所考虑的载体的宿主细胞，优选非人类宿主细胞。如本文所考虑的核酸或含有所述核酸的载体优选地被转化到微生物中，所述微生物则代表了根据实施方案的宿主细胞。用于转化细胞的方法在现有技术中是已确立的，并且为本领域技术人员充分已知。原则上所有细胞(即原核或真核细胞)都适合作为宿主细胞。那些可以按遗传上有利的方式(例如关于转化使用核酸或载体及其稳定建立)来操纵的宿主细胞是优选的，例如单细胞真菌或细菌。另外，优选的宿主细胞因就微生物学和生物技术而言是易于操纵的而著称。这是指例如易于培养性、高生长率、对发酵培养基而言低需求、以及外源蛋白的良好生产和分泌率。在其生产之后，所述多肽可以进一步由产生它们的细胞来修饰，例如通过加入糖分子、甲酰化、胺化等。这种翻译后修饰可以在功能上影响多肽。
另外的实施方案由其活性可以在遗传调节元件的基础上进行调节的那些宿主细胞代表，所述遗传调节元件例如通过在载体上而可用，但是也可以先验地存在于那些细胞中。可以例如通过可控地加入作为激活剂的化合物、通过改变培养条件、或者当达到特定的细胞密度时刺激它们表达。这使得本文所考虑的蛋白质的经济生产成为可能。这样的化合物的一个实例是IPTG，如先前描述的。
优选的宿主细胞是原核或细菌细胞。细菌因短的世代时间和就培养条件而言的低需求而著称。其结果是，可以建立经济的培养方法或生产方法。另外，本领域技术人员在发酵技术中有细菌背景下的丰富经验。革兰氏阴性细菌或革兰氏阳性细菌可以适于特定的生产情况，因为在个体情况下多种原因需要实验确定，如营养来源、产物形成率、时间要求等。
本文所考虑的宿主细胞可以就其对培养条件的要求进行修饰，可以包含其他或另外的选择标记，或者还可以表达其他或另外的蛋白质。特别地，它们可以是那些转基因地表达多种蛋白质或酶的宿主细胞。
然而，宿主细胞也可以是真核细胞，所述真核细胞的特征在于它具有细胞核。再一个实施方案因此由以下宿主细胞代表，所述宿主细胞的特征在于它具有细胞核。与原核细胞不同，真核细胞能够对形成的蛋白质进行翻译后修饰。其实例是真菌，如Actinomycetes，或酵母如Saccharomyces或Kluyveromyces。例如，在蛋白质(结合其合成)旨在经历由此类系统使之成为可能的特定修饰时，这可以是特别有利的。真核系统进行的，特别是与蛋白质合成相关联的修饰是例如低分子量化合物(如膜锚(membrane anchors)或寡糖)的结合。
本文所考虑的宿主细胞例如在非连续或连续系统中以通常的方式进行培养和发酵。在前一种情况下，用宿主细胞接种适合的营养培养基，并且在有待实验确定的时间段之后从培养基中收获产物。连续发酵因实现流平衡而著称，其中在相对长的时间段中，部分细胞死亡但是也有部分更新，并且所形成的蛋白质可以同时从培养基中去除。
本文所考虑的宿主细胞优选地用于生产在本文描述的多肽。
因此，本发明的再一个方面是用于生产本文所述的多肽的方法，所述方法包括培养本文所考虑的宿主细胞；并且从培养基或从宿主细胞中分离多肽。通过诉诸本领域已知的常识和技术，基于所使用的宿主生物体，本领域普通技术人员可以对培养条件和培养基进行选择。
在仍再一个方面中，本发明涉及上文描述的多肽用于蛋白质连接，特别是用于环化一种或多种肽的用途。
还涵盖的是以下多肽用于连接至少两种肽或蛋白质或者环化肽或蛋白质的用途，所述多肽包含以下项、基本上由其组成或由其组成
(i)如在SEQ ID No:3-109中列出的任一个氨基酸序列；
(ii)在其整个长度上与(i)的任一个氨基酸序列具有至少60％、优选至少70％、更优选至少80％、最优选至少90％序列同一性的氨基酸序列；
(iii)在其整个长度上与(i)的任一个氨基酸序列具有至少80％、优选至少90％、更优选至少95％序列同源性的氨基酸序列；或
(iv)(i)-(iii)中任一项的片段，其中所述片段具有连接酶/环化酶活性。
在此类用途的不同实施方案中，基于如在SEQ ID No.3-109中列出的氨基酸序列的根据(i)-(iv)的多肽是基于SEQ ID NO:1的多肽，如上文描述的那些。这特别地涉及在对应于SEQ ID NO:1的位置19、124和166的位置处的保守氨基酸残基N、H和C和/或其活性和功能性。
应当理解的是，虽然在下文通过参考肽底物对在本文描述的酶的用途进行了描述，但是它们可以类似地用于对应的多肽或蛋白质。本发明因此还包括多肽或蛋白质被用作底物的实施方案。这些多肽或蛋白质可以包含如在下文在肽底物的背景下描述的结构基序。还涵盖的是利用保留功能性的肽片段(如人类肽激素的片段)、或肽衍生物(如(主链)修饰的肽，包括例如硫代缩肽)的实施方案。因此，本发明还包括在本文披露的肽底物的片段和衍生物。
在不同实施方案中，待连接的或待环化的肽可以是任何肽，长度典型地是至少10个氨基酸，只要它含有被连接酶/环化酶识别、结合和连接的识别和连接序列即可。待连接的或待环化的肽的这个氨基酸序列可以包含氨基酸残基N或D，优选N。在不同实施方案中。待环化的肽包含氨基酸序列(X)oN/D(X)p，其中X是任何氨基酸，o是1或更大、优选2或更大的整数，并且p是1或更大、优选2或更大的整数。在优选实施方案中，(X)p是H(X)r或HV(X)r，其中r是0或者1或更大的整数。在更优选的实施方案中，所述肽包含氨基酸序列(X)oNH或(X)oNHV。所述氨基酸序列优选地位于待连接的或待环化的肽的C-末端或附近，因为在连接/环化期间所有在N的C-末端的氨基酸都将被切割掉。因此，在所有上述实施方案中，p或r优选地是高达20、优选地高达5的整数。特别优选的是p是2、(X)p优选地是HX或HV、或r是0的实施方案。
在替代性实施方案中，待连接的或待环化的肽可以包含氨基酸序列(X)oN*/D*，其中X是任何氨基酸，o是至少2的整数，并且(N或D残基)的C-末端羧基基团被具有化学式–C(O)-N(R’)2的基团置换，其中R’是任何残基，例如像烷基。在此类实施方案中，N或D残基的末端–C(O)OH基团(在D的情况下优选α-羧基基团)被修饰，以形成基团–C(O)-N(R’)2。这些C-末端酰胺化的D或N残基在本文分别由D*和N*指示。已经发现在本文披露的酶可以切割酰胺基并且将所述N或D残基连接到另一种感兴趣的肽的N-末端或包含N或D残基的相同肽的N-末端。
待连接的肽的N-末端部分优选地包含氨基酸序列X1X2(X)q，其中X可以是任何氨基酸；X1可以是除Pro外的任何氨基酸；X2可以是任何氨基酸，但是优选地是疏水性氨基酸，如Val、Ile或Leu、或Cys；并且q是0或者1或更大的整数。在X1位置优选的是按以下顺序：G＝H>M＝W＝F＝R＝A＝I＝K＝L＝N＝S＝Q＝C>T＝V＝Y>D＝E。“＝”指示对应的氨基酸是类似地优选的，而“>”指示偏好在所述符号之前列出的氨基酸优先于在所述符号之后列出的氨基酸。在X2位置优选的是按以下顺序：L>V>I>C>T>W>A＝F>Y>M>Q>S。在X2位置中较不优选的是P、D、E、G、K、R、N以及H。在X1位置特别优选的是G和H，并且在X2位置特别优选的是L、V、I以及C，如二肽序列GL、GV、GI、GC、HL、HV、HI以及HC。
在优选实施方案中，待连接的或待环化的肽因此在N-到C-末端方向包含氨基酸序列X1X2(X)q(X)oN/D(X)p，其中X、X1、X2、o、p、以及q是如上文定义的，其中o优选地至少是7。在不同实施方案中，(1)q是0并且o是至少7的整数；和/或(2)X1是G或H；和/或(3)X2是L、V、I或C；和/或(4)p至少是2但是不超过22，优选地是2-7，更优选地是H(X)r或HV(X)r，最优选地是HX或HV。在不同实施方案中，(1)q是0并且o是至少7的整数；并且(2)X1是G或H；并且(3)X2是L、V、I或C；并且(4)p至少是2但是不超过22，优选地是2-7，更优选地是H(X)r或HV(X)r，最优选地是HX或HV。
在不同实施方案中，待环化的肽是环状胱氨酸结多肽(cyclic cysteine knotpolypeptide)(特别是大环寡肽)的线性前体形式。大环寡肽是异常稳定的拓扑独特的植物蛋白家族。它们包含被安排在头至尾环化的肽主链中的约30个氨基酸，所述肽主链另外地受与六个保守半胱氨酸残基缔合的胱氨酸结基序的限制。胱氨酸结由两个二硫键及其在结构中形成内环的连接主链区段构建，所述结构被第三个二硫键穿过以形成互锁且交叉支撑的结构。叠加在这个胱氨酸结核心基序上的是良好定义的β-片层和一系列展示出短外露表面环的转角。
大环寡肽在其主链环内表达多样的肽序列，并且具有广泛范围的生物活性。它们因此对制药应用而言具有重要意义。作为这些大环寡肽来源的一些植物被用于本土药物中，包括kalata-kalata，一种含有原型大环寡肽kalata B1(kB1)、来自植物Oldenlandiaaffinis的茶叶，其在非洲用于加速分娩。它们异常的稳定性意指它们在肽基药物设计应用中作为潜在的模板已经吸引了注意。具体而言，将生物活性肽序列接枝到大环寡肽框架中提供了用于稳定化肽基治疗剂的新方法的承诺，由此克服了在将肽用作药物上的主要限制之一。
在不同实施方案中，待环化的肽的长度因此是10个或更多个氨基酸、优选多达50个氨基酸，在一些实施方案中长度是约25至35个氨基酸。待环化的肽可以包含如在SEQ IDNO:110中列出的来自Oldenlandia affinis的大环寡肽kalata B1的前体的氨基酸或由其组成。
在不同实施方案中，待环化的肽包含氨基酸序列(X)nC(X)nC(X)nC(X)nC(X)nC(X)nC(X)nNHV(X)n或由其组成，其中每个n独立地是选自1至6的整数并且X可以是任何氨基酸。此类肽是在六个半胱氨酸残基之间形成胱氨酸键的环状胱氨酸结多肽的前体，如上文描述的，并且其可以通过切割掉C-末端HV(X)n序列并且将N残基(随后C-末端)连接到N-末端残基上而被本文描述的酶环化。
在不同实施方案中，待环化的肽可以包括在US 2012/0244575中披露的线性前体。为此目的，将这个文献通过引用以其全部内容并入本文。
在不同的另外的实施方案，待环化的肽包括但不限于肽毒素和抗微生物肽(如芋螺毒素、死亡素(thanatin)(昆虫抗微生物肽)和组胺素(人类唾液抗微生物肽))的线性前体。可以被环化的其他肽是环状人类或动物肽激素的前体，所述肽激素包括但不限于神经介肽、心血管调节肽α、apelin以及甘丙肽。示例性肽包括在SEQ ID No.111-116和128-132中列出的任一个氨基酸序列或由其组成。
可以使用在本文披露的酶和方法连接或环化的另外的肽包括但不限于促肾上腺皮质激素(ACTH)、肾上腺髓质素、中介素、肾上腺髓质素前体、Adropin、Agelenin、AGRP、Alarin、胰岛素样生长因子结合蛋白5、胰淀素(Amylin)、淀粉体b-蛋白、两亲肽抗生素、LAH4、血管紧张素I、血管紧张素II、A型(心房)利钠肽(ANP)、蜂毒明肽、Apelin、比伐卢定、蛙皮素、赖氨酰缓激肽、B型(脑)利钠肽、C-肽(胰岛素前体)、降钙素、可卡因-苯丙胺调节转录肽(CART)、降钙素基因相关肽(CGRP)、缩胆囊素(CCK)-33、细胞因子诱导的中性粒细胞化学吸引剂-1/生长相关癌基因(CINC)、Colivelin、促肾上腺皮质激素释放因子(CRF)、皮质抑素、C型利钠肽(CNP)、抗栓肽、人类嗜中性粒细胞肽-1(HNP-1)、HNP-2、HNP-3、HNP-4、人类防御素HD5、HD6、人类β防御素-1(hbd1)、hbd2、hbd3、hbd4、δ睡眠诱导肽(DSIP)、皮离蛋白-1L、强啡肽A、弹力素、目录肽(Endokinin)C、目录肽D、b-促脂解素、g-内啡肽、内皮素-1、内皮素-2、内皮素-3、大-内皮素-1、大-内皮素-2、大-内皮素-3、恩夫韦地、艾塞那肽(Exendin)-4、MBP、髓磷脂少突胶质细胞蛋白(MOG)、Glu-纤维蛋白肽B、甘丙肽、甘丙肽样肽、大胃泌素(人类)、抑胃多肽(GIP)、胃泌素释放肽、饥饿素(Ghrelin)、胰高血糖素、胰高血糖素样肽-1(GLP-1)、GLP-2、生长激素释放因子(GRF，GHRF)、鸟苷素、尿鸟苷素、尿鸟苷素异构体A、尿鸟苷素异构体B、铁调素(Hepcidin)、肝表达的抗菌肽(LEAP-2)、Humanin、加入肽(Joining Peptide)(rJP)、Kisspeptin-10、Kisspeptin-54、利拉鲁肽、LL-37(人源杀菌肽)、促黄体激素释放激素(LHRH)、蛙皮素1、蜂毒肽、a-交配因子、肥大细胞脱颗粒(MCD)肽、黑色素浓集激素(MCH)、a-黑素细胞刺激激素(α-MSH)、中期因子、胃动素、神经内分泌调节肽1(NERP1)、NERP2、神经激肽A、神经激肽B、神经介肽B、神经介肽C、神经介肽S、神经介肽U8、Neuronostatin-13、神经肽B-29、神经肽S(NPS)、神经肽W-30、神经肽Y(NPY)、神经降压素、孤啡肽、痛稳素、肥胖抑制素、食欲肽(Orexin)-A、骨钙素、催产素、儿茶酚抑素、嗜铬颗粒蛋白A、甲状旁腺激素(PTH)、肽YY、垂体腺苷酸环化酶激活多肽38(PACAP-38)、血小板因子-4、菌丝霉素、多效生长因子、催乳素释放肽、多聚谷氨酰化的RF酰胺肽(QRFP)、RF酰胺相关肽-1、胰泌素、血清胸腺因子(FTS)、钾钠ATP酶抑制剂-1(SPAI-1)、生长抑素、生长抑素-28、顶压素(Stresscopin)、尿皮质素(Urocortin)、P物质、锯鳞蝰素(Echistatin)、肠毒素STp、广西毒素-1E、尾加压素II、血管活性肠肽(VIP)、以及加压素及其片段和衍生物。上述肽可以具有人类或动物(如大鼠、小鼠、猪)来源。它们全部为本领域普通技术人员所熟知并且其氨基酸序列是容易地可获得的。
在不同的其他实施方案中，多于50个氨基酸长度的多肽或蛋白质被用作环化底物。在这样的反应中，所述多肽/蛋白质可以通过将其C-末端连接到其N-末端来环化。
在不同实施方案中，通过本发明的酶连接两种或更多种肽。这可以包括形成由两种或更多种肽组成的大环，优选的是大环二聚体。待连接的肽可以是任何肽，只要它们中的至少一种含有被连接酶/环化酶识别、结合和连接的识别和连接序列即可。已经结合环化策略在上文描述了适合的肽。相同的肽也可以用于连接到另一种可以相同或不同的肽上。待连接的肽之一可以例如是具有酶活性或另一种生物功能的多肽。待连接的肽还可以包括标记物肽或包含可检测标记物的肽，如荧光标记或生物素。根据此类实施方案，具有生物活性的多肽可以被融合到可检测标记物上。在不同实施方案中，待连接的肽中的至少一种具有25个氨基酸或更多个、优选50个氨基酸或更多个的长度(并且因此在本发明的意义上可以是“多肽”)。
待连接的肽可以包含在SEQ ID No.117至127中列出的任何氨基酸序列或由其组成。优选的待连接以形成(大环)二聚体的肽包括具有在SEQ ID No.117-121的任一项中列出的氨基酸序列的肽。优选的待(与一种C-末端肽)连接以形成线性融合肽的N-末端肽包括具有在SEQ ID No.112、115和117的任一项中列出的氨基酸序列的肽。优选的待(与一种N-末端肽)连接以形成线性融合肽的C-末端肽包括具有在SEQ ID No.113、114和116的任一项中列出的氨基酸序列的肽。
待连接的或待环化的肽还可以是这样的融合肽或多肽，其中含Asx的标签已经被C-末端融合到感兴趣的待连接的或待融合的肽上。含Asx的标签优选地具有氨基酸序列N/D(X)p，其中X是任何氨基酸，并且o和p彼此独立地是1或更大、优选2或更大的整数。在优选实施方案中，所述标签包含(C-末端)氨基酸序列NH或NHV或者由其组成。可替代地，酰胺化的N或D(如上文定义的N*或D*)可以被融合到待连接的或待融合的肽或多肽的C-末端。这种融合肽或多肽所连接的另一种肽可以是如上文定义的。可替代地，所述融合肽或多肽可以通过在其C-末端与N-末端之间形成键来环化。在一个实施方案中，所述融合肽或多肽可以是融合到氨基酸序列NHV(SEQ ID NO:133)的C-末端标签上的绿色荧光蛋白，并且所连接的肽可以是氨基酸序列GIGK(生物素化的)R(SEQ ID NO:134)的生物素化的肽。通常，可以使用在本文描述的方法和用途而与肽(如带有信号传导或可检测部分的肽)连接或环化的多肽和蛋白质包括但不限于抗体、抗体片段、抗体样分子、抗体模拟物、肽适体、激素、各种治疗性蛋白等。
在不同实施方案中，连接酶活性被用于将带有可检测部分(如荧光基团包括荧光素，如异硫氰酸荧光素(FITC)，或香豆素如7-氨基-4-甲基香豆素)的肽与多肽或蛋白质(如在上文提及的那些)融合。在不同实施方案中，所述蛋白质可以是抗体片段如人类抗-ABLscFv，例如具有在SEQ ID NO:146中列出的氨基酸序列，或是抗体模拟物如darpin(设计的锚蛋白重复蛋白)，例如对人类ERK有特异性的darpin，例如具有在SEQ ID NO:147中列出的氨基酸序列。
在仍另一个方面中，本发明涉及用于使肽、多肽或蛋白质环化的方法，该方法包括结合本发明的用途在允许环化所述肽的条件下用上文描述的具有连接酶/环化酶活性的多肽孵育所述肽、多肽或蛋白质。
在仍再一个方面中，本发明涉及用于连接至少两种肽、多肽或蛋白质的方法，该方法包括结合本发明的用途在允许连接所述肽的条件下用上文描述的多肽孵育所述肽、多肽或蛋白质。
在不同实施方案中，待根据这些方法环化的或连接的肽、多肽和蛋白质被类似地定义为待根据上文描述的用途环化的或连接的肽、多肽和蛋白质。
在本文描述的方法和用途中，可以按1:100或更高、优选1:400或更高、更优选至少1:1000的摩尔比来使用酶和底物。
典型地使反应在允许最佳酶活性的温度(通常在环境温度(20℃)与40℃之间)下在适合的缓冲液体系中进行。
在上文描述的方法和用途中，具有连接酶/环化酶活性的多肽可以被固定在适合的支持材料上。适合的支持材料包括在层析柱中使用的各种树脂等。支持体可以具有珠粒的形式，或者可以是更大的结构(如微量滴定板)的表面。固定允许非常容易且简单地与底物接触，并且在合成之后容易地分离酶和底物。如果具有酶促功能的多肽被固定在固体柱材料上，则连接/环化可以是连续过程和/或可以使底物/产物溶液在柱上循环。
因此，在一个方面中，本发明还包括下述固体支持材料，其包含固定在其上的根据本发明的分离的多肽。固体支持材料可以包括聚合物树脂，优选地处于微粒形式，如上文提及的那些。分离的多肽可以通过共价或非共价相互作用被固定在固体支持材料上。
在示例性实施方案中，具有连接酶/环化酶活性的多肽被糖基化并且可以借助伴刀豆球蛋白A(Concanavalin A,Con A)(一种从Canavalia ensiformis(刀豆)中分离的凝集素(碳水化合物结合蛋白))进行固定。它特异性地结合到含有α-D-甘露糖和α-D-葡萄糖的生物分子(包括糖蛋白和糖脂)上。所述ConA蛋白以固定形式用于亲和柱上以固定糖蛋白和糖脂。因此，在不同实施方案中，具有连接酶/环化酶活性的分离的多肽被糖基化并被非共价地结合到偶联在固体支持材料表面上的碳水化合物结合部分(优选伴刀豆球蛋白A)上。
上文描述的固体支持材料可以用于至少一种底物肽的柱上环化和/或连接，或者用于环化或连接至少一种底物肽的方法中，包括在允许环化和/或连接所述至少一种底物肽的条件下使包含所述至少一种底物肽的溶液与上文描述的固体支持材料接触。底物肽是上文描述的那些并且还包括以上多肽底物。
本发明还涵盖包含编码本文所述的具有蛋白质连接酶和/或环化酶活性的多肽的核酸分子的转基因植物。多肽优选地不天然地存在于所述植物中。因此，本发明还重点包括表达根据本发明的异源多肽的转基因植物。
在不同实施方案中，此类转基因植物可以进一步包含至少一种编码一种或多种待环化的肽或一种或多种待连接的肽的核酸分子。这些可以是如上文结合本发明的用途和方法定义的肽。在一个实施方案中，待环化的肽是环状胱氨酸结多肽的线性前体形式，例如像上文定义的那些。待环化的肽或多肽的这些前体可以天然地存在于所述植物中，但还优选地是人为引入的，即编码它们的核酸是异源的。
归因于酶及其底物的共表达，此类转基因植物可以因此直接产生环化的感兴趣的肽。
在本文相对于多肽和核酸披露的所有实施方案都可类似地应用于在本文描述的用途和方法中，反之亦然。
本发明由以下非限制性实施例进一步说明。
实施例
材料。Z-AAN-AMC(N-苄氧羰基-Ala-Ala-Asn-7-酰胺基-4-甲基香豆素)和肽底物由GL Biochem(上海)合成。使每种肽底物的氧化折叠在含有50％乙腈、100mM碳酸氢铵、3mM还原型谷胱甘肽的缓冲液(pH 8.0)中在30mM的肽浓度下进行18h。刀豆豆荚蛋白购自Takara Bio(日本)。从O.affinis的地上部分分离天然kB1肽并且通过使用RP-HPLC进行纯化。豆荚蛋白特异性LP-1探针由Matthew Bogyo(斯坦福大学)提供。
登录代码。butelase 1的核苷酸序列已经在登录号KF918345下保存在GenBank数据库中。
实施例1：天冬酰胺酰基内肽酶和肽环化酶活性的体外筛选
通过在缓冲液A中在100mM的浓度下使用荧光底物Z-Ala-Ala-Asn-AMC(Z-AAN-AMC)(一种对豆荚蛋白具选择性的荧光底物)(Kembhavi，A.A.，Buttle，D.J.，Knight，C.G.&Barrett，A.J.The two cysteine endopeptidases of legume seeds：purification andcharacterization by use of specific fluorometricassays.Arch.Biochem.Biophys.303，208-213(1993)；Sojka，D.等人IrAE-An asparaginylendopeptidase(legumain)in the gut of the hard tick Ixodesricinus.Int.J.Parasitol.37，713-724(2007))确定天冬酰胺酰基内肽酶(AEP)活性。用380nm的激发波长和460nm的发射波长测量发射的荧光。
在第一实验中，在上文描述的条件下用Z-AAN-AMC孵育蝶豆的粗提物。观察到460nm下的荧光强度大增，指示推定的豆荚蛋白的存在。
然后对环化酶活性进行测定。通常，使体外环化试验在含有缓冲液A、0.125mMbutelase 1和变化的肽浓度(0.5至400mM)的50-ml反应混合物中进行。通过280nm下的UV吸光度估计酶浓度。使每个反应在37℃下一式三份地进行，并且通过加入5ml的1M HCl溶液淬灭。通过使用反相C18分析柱(150x 2.1mm，Vydac)在Nexera UHPLC系统(日本岛津公司(Shimadzu))上分离肽。通过将剩余的线性前体或环化产物的HPLC-峰面积转化为浓度来计算环化速度。通过MALDI-TOF MS和MS/MS(ABI 4800MALDI TOF/TOF)分析每个HPLC峰的类别。
使用肽底物kB1-NHV(SEQ ID NO:110)对蝶豆粗提物的环化酶活性进行测定，所述肽底物是作为前肽的在C-末端具有His-Val序列的大环寡肽kB1的31个残基的、线性且经氧化折叠形式(表1和图1)。在我们的试验中将Kalata B1(kB1)(发现于Oldenlandia affinis中但未发现于蝶豆中的原型大环寡肽)选择为底物，以将它与由蝶豆产生的天然大环寡肽区分开。His-Val基序在蝶豆的大环寡肽前体中是保守的，并且已经显示C-末端二肽对于大环寡肽的生物合成而言是足够的(Nguyen，G.K.，Lim，W.H.，Nguyen，P.Q.&Tam，J.P.NovelCyclotides and Uncyclotides with Highly Shortened Precursors from Chassaliachartacea and Effects of Methionine Oxidation onBioactivities.J.Biol.Chem.287，17598-17607(2012).Conlan，B.F.等人Insights intoProcessing and Cyclization Events Associated with Biosynthesis of the CyclicPeptide Kalata B1.J.Biol.Chem.287，28037-28046(2012))。用蝶豆的提取物处理kB1-NHV产生了新肽，所述新肽匹配经计算的天然环状kB1的质量(图2a、2b)。通过以下各项，这种肽产物被进一步确认为是环状kB1：(1)在RP-HPLC中与天然环状kB1共洗脱(图3)；(2)导致18Da的质量增加的胰蛋白酶消化暗示环状主链，和MS/MS分析确认了kB1序列和Asn-Gly作为连接位点(图4)；和(3)针对环化的肽和天然环状kB1显示出相同的化学位移的1D NMR(图5)。对于kalata B1的1D NMR谱，在95％H2O/5％D2O(pH 4.3)中制备0.1mM浓度的天然的和butelase环化的kB1肽。在配备有冷冻探针的600MHz NMR光谱仪(Bruker)上记录两种肽的1D 1H谱。这些结果指示在蝶豆的粗提物中存在能够进行肽大环化的推定的连接酶。
表1.由butelase 1介导的肽底物的环化产率
使试验在37℃和1:400的酶肽比(0.125μM butelase 1:50μM肽)下进行。*指示C-末端的酰胺化的肽。aS-脲甲基化的kB1-NHV。
实施例2：butelase 1的分离、纯化、鉴定和表征。
由Z-AAN-AMC指导的分离肽连接酶的尝试是不成功的。在粗提物的HPLC分离之后给出强荧光强度的级分无法环化kB1-NHV。相反，观察到对应于kB1的线性形式的、His-Val被水解的肽。然后直接使用kB1-NHV作为底物对所有经HPLC分离的级分进行筛选，并且在缺乏荧光的级分中发现了环化酶活性(图2c)。这个结果证明环化酶活性与AEP活性是分开的。作为对照，商业刀豆豆荚蛋白无法环化kB1-NHV并且仅产生线性形式的kB1(图2d)。
以若干层析步骤纯化推定的连接酶，以在SDS-PAGE上给出38kDa的单个蛋白质带(图6a)。为了分离和纯化，将300g的蝶豆荚果用500ml的提取缓冲液(20mM磷酸钠、1mMEDTA、1mM PMSF、5mM b-巯基乙醇(b-ME)，pH 6.0)匀化。在4℃下进行提取，以使蛋白质降解最小化。将匀浆离心并过滤，以除去植物碎片。将硫酸铵加入上清液中，至达到20％的饱和度。将沉淀的蛋白质丢弃，并且继续将硫酸铵加入上清液中至达到85％的饱和度。离心之后，将上清液丢弃，并且将沉淀的蛋白质重新溶解于300ml的提取缓冲液中。使用10kDa截留透析管，将溶解的样品用6升的提取缓冲液透析过夜。将经透析的样品离心并过滤，以给出蝶豆的粗提物。将这种粗提物施加到含有100ml的Q-Sepharose Fast Flow阴离子交换树脂(GE Healthcare)浆料的快速柱上。将柱用800ml的缓冲液A(20mM磷酸盐缓冲液、1mM EDTA、5mM b-ME，pH 6.0)洗涤并用400ml的缓冲液B(20mM磷酸钠、1mM EDTA、5mM b-ME、200mMKcl,pH 6.0)洗脱。使用10kDa截留离心过滤器装置(Amicon Ultra，Millipore)将洗脱物浓缩至3ml的终体积。使用BioSuite HPLC柱(300x 21.5mm，Waters)，对经浓缩的样品经受进行的尺寸排阻层析。将具有肽环化酶活性的级分取出，并且使用分析型PolyWAX HPLC柱(200x 4.6mm，PolyLC)通过阴离子交换层析进一步纯化。通过SDS-PAGE和银染分析酶纯度。可以从300g的植物材料获得大约0.4mg的butelase 1。
在变性条件下通过SDS-PAGE分析经纯化的butelase 1。将凝胶进行银染，切下蛋白质带并使其经受如先前描述的胶内胰蛋白酶消化(Gharahdaghi，F.，Weinberg，C.R.，Meagher，D.A.，Imai，B.S.&Mische，S.M.Mass spectrometric identification ofproteins from silver-stained polyacrylamide gel：A method for the removal ofsilver ions to enhance sensitivity.Electrophoresis20，601-605(1999))。胶内胰蛋白酶消化给出五个优势肽片段，然后通过MALDI-TOF MS/MS对所述肽片段进行测序(图7)。针对由Beijing Genomics Institute提供的蝶豆的转录组数据对这些片段进行BLAST检索，发现它们匹配被指定为butelase 1的新颖蛋白质的单个序列(图6c)。所述酶在4℃下历时30天仍是稳定的，具有最小的活性损失。它在水中是相对可溶的，其已经达到10mg/ml的浓度。
基于蝶豆转录组中的EST序列，预测butelase 1由482个残基组成并且具有53kDa的质量，而经纯化的活性酶大约是38kDa，表明它通过蛋白水解加工进行了翻译后修饰(图6c)。用PNGase F或糖肽酶A孵育butelase 1没有产生分子量变化，指示butelase1未被N-糖基化(数据未示出)。针对NCBI非冗余蛋白质数据库的BLASTp检索显示，butelase 1与豆荚蛋白家族的若干成员具有高序列同源性。Butelase 1与来自Glycine max的豆荚蛋白样蛋白(NCBI参考序列：XP_003525979)和来自Vigna mungo的VmPE-1(GenBank：BAA76744.1)具有最高的同源性，序列同一性分别是71％和70％。这个结果强烈地表明butelase 1是豆荚蛋白家族的新成员。butelase 1的酶学分类进一步得到了荧光探针LP-1(一种对豆荚蛋白有特异性的aza-Asn环氧化物探针)对其标记的支持(图6b和图8)(Lee，J.&Bogyo，M.Development of Near-Infrared Fluorophore(NIRF)-Labeled Activity-BasedProbes for in Vivo Imaging of Legumain.ACS Chem.Biol.5，233-243(2010))。
豆荚蛋白被产生为无活性酶原，其经历自蛋白水解激活以释放N-和C-末端前域。Edman测序揭示了VEGTR作为butelase 1的N-末端序列。预测C-末端加工位点出现在Asn383与Ser384之间，这是基于38kDa的表观分子量，和其他豆荚蛋白(如来自Vicia sativa的蛋白质酶B和刀豆豆荚蛋白)的自切割位点(图6c)(Becker，C.等人Purification，CdnaCloning and Characterization of Proteinase-B，an Asparagine-SpecificEndopeptidase from Germinating Vetch(Vicia-Sativa L)Seeds.Eur.J.Biochem.228，456-462(1995)；Abe，Y.等人Asparaginyl Endopeptidase of Jack Bean-Seeds-Purification，Characterization，and High Utility in Protein-SequenceAnalysis.J.Biol.Chem.268，3525-3529(1993))。
基于人类豆荚蛋白(仅有的晶体结构已知的豆荚蛋白家族成员)的酶原(Sali，A.&Blundell，T.L.Comparative protein modelling by satisfaction of spatialrestraints.J.Mol.Biol.234，779-815(1993)；Dall，E.&Brandstetter，H.Mechanisticand structural studies on legumain explain its zymogenicity，distinctactivation pathways，and regulation.Proc.Natl.Acad.Sci.U.S.A.110，10940-10945(2013))，使用MODELLER(https://salilab.org/modeller/)来构建butelase 1的同源性模型。butelase 1的酶原(V42-I468)与人类豆荚蛋白具有37.8％的序列同一性。所构建的butelase 1模型与人类豆荚蛋白的模板结构一致性良好，对于链Cα而言具有的RMSD(图6d)。
先前的研究将人类豆荚蛋白的酶原定义为三个结构部分：AEP活性结构域，活性肽区域，以及豆荚蛋白稳定化和活性调节(LSAM)结构域。后两个结构域在人类豆荚蛋白中的酶激活期间被自动切割。类似地，建模的butelase 1结构也可以被分为三个部分：推定的AEP活性结构域(海蓝色，V42-T318)，活性肽区域(品红色，D319-N383)以及LSAM结构域(灰色，S385-I468，其在butelase 1的最终活性形式中被排除)。总的来说，与人类豆荚蛋白的AEP活性结构域相比，butelase 1的AEP活性结构域保留了49.8％的序列同一性(V42-T318)，并且展示出良好的催化三联体(Asn59、His165和Cys207)结构比对(图6d)。
使用来源于不同植物家族的两种非天然线性肽底物，31-残基kB1-NHV和16-残基SFTI-NHV(SEQ ID NO:135)，通过HPLC和MS分析来确定作为肽环化酶的butelase1的动力学。尽管是具有不同长度和序列的非天然底物，但是butelase 1以卓越的产率有效地环化这些肽(表1)。
环化反应的RP-HPLC踪迹揭示了在1:400的酶肽比下，butelase 1在6min内将约40％的kB1-NHV转化为环状kB1，并且在45min内达到>95％的转化率(图9a)。使用GraphPadPrism从Michaelis-Menten图计算的针对kB1-NHV的butelase 1的表观动力学参数对于kcat而言是2.28±0.05s-1，对于Km而言是213±10mM，并且对于催化效率(kcat/Km)而言是10,700M-1s-1(图9b)。另外，在高底物浓度(>400mM)下观察到kB1的58-残基的环二聚体(<10％)，表明butelase 1能够执行长肽的分子间连接和环化(图10)。
通过SFT1-NHV，butelase 1还示出了>95％的转化产率，其中kcat是0.6±0.02s-1，Km是51±4mM，并且催化效率是11,700M-1s-1(图9c)。这些数据表明butelase 1可以环化各式各样的肽底物。
由于SFTI和kB1两者均在自然界中作为由二硫键稳定化的环肽存在，随后确定了通过butelase 1进行环化反应是否要求由二硫键提供的构象援助。用碘乙酰胺对经还原的kB1-NHV进行S-烷基化，给出了SA-kB1-NHV。用0.125mM butelase 1处理50mM经S-烷基化的肽(SA-kB1-NHV)在12min内导致了>95％转化成其环状形式(表1)。动力学分析显示出与kB1-NHV相比，SA-kB1-NHV的催化效率改进50倍(图9d)。这个结果证明通过butelase 1进行的肽环化不要求二硫键。
为了研究C-末端前肽的P1'和P2'位置的要求，合成了四种kB1-NHV类似物(表1)。具有比kB1-NHV长的前肽的类似物展示出小的环化速率下降，其中针对kB1-NHVI(SEQ IDNO:136)和kB1-NHVIA(SEQ ID NO:137)的催化效率分别是4032和2971M-1s-1(表1和表2)。相比之下，butelase 1在环化两种截短的缺乏Val或His-Val的类似物(SEQ ID NO:138；SEQID NO:139)方面效率显著较低，其中4h之后环状kB1产率<10％，并且30h之后反应是不完全的(图11)。这个结果指示，C-末端HV二肽为通过butelase 1进行有效的环化反应所必需。
表2.针对不同肽底物的butelase 1的动力学参数
为了确定butelase 1在P1位置处的底物特异性，通过用Ala或密切相关残基如Asp、Glu和Gln单独地替换保守Asn残基来制备kB1-NHV的类似物底物(表1；SEQ ID No.140、141、142、143)。在用butelase 1孵育4h之后没有观察到kB1-AHV、kB1-QHV或kB1-EHV的环化(图12)。Butelase 1能够环化kB1-DHV，但比kB1-NHV慢大约百倍，并且4h之后有少于10％的环化产物。类似地，我们比较了butelase 1对SFT1-NHV和SFT1-DHV(SEQ ID NO:144)的活性。Butelase 1环化两种肽底物，但是与SFTI-DHV相比更显著有效地环化SFTI-NHV。这些结果证明C-末端NHV三肽标签为通过butelase1进行的环化所必要且充分的。
为了提供通用性(generality)的证据，使用来源于芋螺毒素(MrIA；SEQ ID NO:111)、死亡素类似物(昆虫抗微生物肽；SEQ ID NO:112)，以及组胺素-1a、组胺素-1b、组胺素-3a、和组胺素-3b(人类唾液抗菌蛋白；SEQ ID No.113-116)的底物检查了butelase1是否可以环化非植物来源的蛋白质(表3)。Butelase 1有效地环化了所有被测试的肽。
表3.针对环化测试的肽的连接产率
为了确定为什么Z-AAN-AMC在针对butelase 1的试验中没有用，用50mMZ-AAN-AMC孵育0.125mM纯化的酶。在孵育30h之后没有观察到明显的荧光强度增加，指示butelase 1不水解Z-AAN-AMC。RP-HPLC分析显示形成了<3％的水解产物(图13)。作为阳性对照，刀豆豆荚蛋白在相同的实验条件下完全水解Z-AAN-AMC。这个结果表明butelase 1已经进化为作为连接酶而非蛋白酶起作用。
kB1的环二聚化表明butelase 1能够介导分子间肽连接。值得指出的是从蝶豆中分离的>24种天然大环寡肽的高序列多样性还提供了诱人的提示，即butelase 1是具有广泛底物特异性的混杂酶(promiscuous enzyme)。为了定义受体亲核试剂的N-末端特异性，KALVINHV(SEQ ID NO:122)被用作模型肽并且评估了其与XIGGIR(X＝20种天然地存在的氨基酸(G,A,V,L,I,F,Y,W,H,R,K,S,T,D,E,N,Q,P,C,M)中的任一种；SEQ ID NO:123)的连接效率。使反应在0.1mM butelase 1、50mM KALVINHV和1mM XIGGIR的存在下进行。Butelase1以广泛特异性有效地介导分子间肽连接，在P1″位置处接受大部分天然氨基酸，Pro和酸性氨基酸如Asp和Glu除外(图15a)。对大部分肽而言，在孵育10min内连接产率达到60％-80％，观察到<5％的天冬酰胺酰基键水解。
为了定义P2″位置处的特异性，合成了第二肽文库：LXGGIR(SEQ ID NO:124)(X＝20种天然地存在的氨基酸中的任一种)。与P1″位置相比，Butelase 1在P2'’处展现出更加严格的要求，并且对疏水性氨基酸特别是Ile、Leu和Val展示出高偏好(图15b)。这个结果还解释了butelase 1对芋螺毒素和组胺素-3的高催化效率。
另外，还针对肽YRNHV(SEQ ID NO:125)+GLPVR(SEQ ID NO:126)和TRNHV(SEQ IDNO:127)+GLPVR(SEQ ID NO:126)测试了连接活性。在孵育10min内连接产率达到60。
为了证明butelase 1的通用性，选择了五种大小范围从26至40个残基的不含半胱氨酸的肽激素。四个序列来源于人类肽(神经介肽U(SEQ ID NO:128)、心血管调节肽a(SEQID NO:129)、apelin(SEQ ID NO:131)、和甘丙肽(SEQ ID NO:132))，并且一个序列来源于大鼠(神经介肽U；SEQ ID NO:130)(表4)。人类甘丙肽和神经介肽U含有内在Asn残基，因此能够进行“无痕”连接，在最终环化产物中不留下任何另外的分选序列。对于其他肽，另外的Asn-His-Val序列被加到C-末端，并且Gly或Gly-Ile被加到N-末端，作为接头序列。使环化反应在含有50μM肽和0.1μM butelase 1(0.002摩尔当量)的50μl反应混合物中在37℃下进行。HPLC和质谱用于监测反应。值得注意地，针对所有被测试的肽，butelase 1在5min内达到>95％的环化产率(图16)。这些肽是随机选择的且没有序列同源性的事实表明了butelase 1的多样性和最低底物要求。
表4
接下来检查了这些肽底物的环化动力学。使用GraphPad Prism从Michaelis-Menten图计算butelase 1的表观动力学参数(表5)。催化效率落入1x 105至1.3x 106M-1s-1的范围内，这与我们先前的对富半胱氨酸肽的研究是一致的。这个结果确认了二硫化物以及进而由多种二硫化物维持的折叠结构不是通过butelase 1进行的环化所需的。
表5
另外，进行具有C-末端NHV-标签(SEQ ID NO:133)的绿色荧光蛋白与短肽GIGK(生物素)R(SEQ ID NO:134)的试验连接，以证明butelase 1用于蛋白质标记的应用。为了所述反应，将具有NHV标签的50μM GFP、0.125μM butelase 1和1mM GIGK(生物素)R在37℃下孵育30分钟。结果示出图17中。
为了证明butelase 1可以环化蛋白质，构建了在C-末端以Asn-His-Val基序结束并且在N-末端以Gly-Ile开始的修饰的GFP(SEQ ID NO:145)。使环化反应在25μM GFP和0.1μM butelase 1(0.004摩尔当量)的存在下进行。环化反应以>90％的产率在15min内完成，如通过SDS page和高分辨率ESI-MS监测的(图18)。为了比较，分选酶介导的GFP环化需要24小时的孵育和1摩尔当量的分选酶A。使用GFP作为模型蛋白，butelase 1的催化速率因此比分选酶A快近10,000倍。这个结果证明butelase 1是有希望的潜力巨大的连接酶并且可以为肽和蛋白质的环化提供替代性方法。
为了研究butelase 1向蛋白质中引入官能团(如生物素和荧光团)的能力，进行C-末端和N-末端连接，并且通过不同技术对结果进行分析。用于C-末端连接，所测试的底物是ABL-Mono(合成的抗ABL蛋白的人类scFv片段；SEQ ID NO:146)、和ERK-Darp(合成的对ERK有特异性的人类抗体模拟物(darpin)；SEQ IDF NO:147)(表6)。用于N-末端连接，所测试的底物是泛素蛋白(SEQ ID NO:148)、和肽1(YKNHV，SEQ ID NO:149)或其巯基乙酸变体(硫代缩肽)(YKN-巯基乙酸-V)。
表6
为了研究butelase 1的C-末端连接能力，在butelase 1的存在下，用含荧光的肽GIR-AMC(AMC＝7-氨基-4-甲基香豆素)孵育具有14469.2Da MW的ABL-Mono和具有20270.2Da MW的ERK-Darp。通过MS监测反应(图19)。在14718.4Da和20511.1Da下检测到的峰是ABL-Mono和ERK-Darp的连接产物，其中加入了GIR-AMC并且去除了His、Val和水分子。在20分钟孵育之后，ERK-Darp达到了几乎90％的转化产率。
归因于ERK-Darp、GFP-NHV及其与FITC标记的肽FITC-GKNHV(SEQ ID NO:150)的连接产物的MW较大，通过MS进行分析是不够准确的。使用专门用于电离化大分子的ESI-MS(Ho等人，Clin Biochem.Rev.(2003)24(1)，3-12)来检查大的连接产物。对于连接反应，在42℃下用1mM FITC-GKNHV和50nM butelase 1将50μM ERK-Darp和50μM GFP-NHV孵育10分钟。将ESI-MS的产物样品首先通过UPLC分离，以降低盐浓度。通过ESI prot 1.0程序分析ESI-MS的结果。通过ESI-MS获得的ERK-Darp的MW是20205±1.5Da，与理论MW仅具有239Da的差异。推定的连接产物的MW是20453±1.6Da，并且这证明了蛋白质连接成功。GFP-NHV和推定的连接产物的MW分别是29720.0±0.7Da和29969.3±0.7Da(图20)。
对于N-末端连接，反应条件是：100μM泛素，0.1μM butelase 1，500μM肽1，在42℃下进行孵育。150分钟之后的反应产率是82％。通过HPLC和MS监测反应(图21)。
已经显示肽树枝状聚合物(dendrimers)具有增加的活性，这是由于增加的结合亲和力。为了测试使用butelase 1作为连接酶产生树枝状聚合物肽的可能性，进行模型肽YRNHV(SEQ ID NO:125)与二聚体肽G2K(两个通过K残基连接的GIG序列)的连接(图22)。将50μM G2K、20nM butelase 1和250μM YRNHV肽在37℃下孵育1h。质谱被用于在孵育结束时监测反应(图22)。结果提供了以下概念验证，即butelase1能够充当用于肽二聚化的连接酶。
还用肽GV-10、SV-10、HV-10、EV-10和RV-10(SEQ ID No.117-121)测试了二聚化能力。结果示于表7中。
表7小肽的二聚化和环化
实施例3：N-末端环化特异性
为了进一步研究butelase 1的关于其环化酶活性的N-末端特异性，合成了三个肽文库并针对环化对其进行测试：
肽文库1：XLYRRGRYLRRNHV(SEQ ID NO:157)
肽文库2：XRLYRGRYLRRNHV(SEQ ID NO:158)
肽文库3：GXLYRGRYLRRNHV(SEQ ID NO:159)
在上述肽文库中，X表示20种天然氨基酸中的任一种，如上文所定义的。环化肽具有与上文给定相同的序列，除外的是其中C-末端残基HV已经被切割掉并且C-末端N被共价地连接到对应肽的N-末端氨基酸上。使反应在50nM butelase 1、50μM肽的存在下在42℃下进行60min。这种环化活性的结果在图23a)-c)中被示为三个肽文库的环化产率。
通过这个实验，可以得出结论当P2”残基(位置2处的残基，如从N-向C-末端计算的)是Leu/Val/Ile/Cys中的任一种时，则P1”残基(即位置1处的末端残基)是不重要的，这与我们从KALVINHV与XIGGIR的分子间连接获得的结果(实施例2)相似。进一步地，当P1”残基是Gly时，则P2”可以是任何残基并且仍允许有效的环化。
实施例4：将Butelase 1可逆地固定在伴刀豆球蛋白A树脂上用于柱上肽环化
伴刀豆球蛋白A(Con A)是一种从Canavalia ensiformis(刀豆)中分离的凝集素(碳水化合物结合蛋白)。它特异性地结合含有α-D-甘露糖和α-D-葡萄糖的生物分子(包括糖蛋白和糖脂)。Butelase 1是重组表达的并且以糖基化形式被分离为约37kDa的蛋白质(数据未示出)。将它通过其碳水化合物部分固定在 ConA-1S亲和柱(ThermoScientific)上。经固定的butelase 1是完全功能性的并且能够催化SFTI-1、kB1(kalataB1)和芋螺毒素的柱上环化。使反应在50μg经固定的butelase 1和50μM的肽底物的存在下进行。图示了(a)芋螺毒素GV-17(SEQ ID NO:111)、(b)SFTI-NHV(SEQ ID NO:135)、(c)kB1(SEQ ID NO:110)的环化的UPLC曲线示出图24中。
序列表
<110> 南洋理工大学
<120> Asx特异性蛋白质连接酶
<130> P109209
<150> US 61/983,729
<151> 2014-04-24
<160> 159
<170> PatentIn version 3.5
<210> 1
<211> 342
<212> PRT
<213> 蝶豆（Clitoria ternatea）
<400> 1
Val Glu Gly Thr Arg Trp Ala Val Leu Val Ala Gly Ser Lys Gly Tyr
1 5 10 15
Val Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Ile Leu
20 25 30
Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp
35 40 45
Asp Ile Ala Tyr Asn Glu Ser Asn Pro His Pro Gly Val Ile Ile Asn
50 55 60
His Pro Tyr Gly Ser Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Val
65 70 75 80
Gly Glu Asp Ile Asn Pro Pro Asn Phe Tyr Ala Val Leu Leu Ala Asn
85 90 95
Lys Ser Ala Leu Thr Gly Thr Gly Ser Gly Lys Val Leu Asp Ser Gly
100 105 110
Pro Asn Asp His Val Phe Ile Tyr Tyr Thr Asp His Gly Gly Ala Gly
115 120 125
Val Leu Gly Met Pro Ser Lys Pro Tyr Ile Ala Ala Ser Asp Leu Asn
130 135 140
Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Ile Val
145 150 155 160
Phe Tyr Val Glu Ser Cys Glu Ser Gly Ser Met Phe Asp Gly Leu Leu
165 170 175
Pro Glu Asp His Asn Ile Tyr Val Met Gly Ala Ser Asp Thr Gly Glu
180 185 190
Ser Ser Trp Val Thr Tyr Cys Pro Leu Gln His Pro Ser Pro Pro Pro
195 200 205
Glu Tyr Asp Val Cys Val Gly Asp Leu Phe Ser Val Ala Trp Leu Glu
210 215 220
Asp Cys Asp Val His Asn Leu Gln Thr Glu Thr Phe Gln Gln Gln Tyr
225 230 235 240
Glu Val Val Lys Asn Lys Thr Ile Val Ala Leu Ile Glu Asp Gly Thr
245 250 255
His Val Val Gln Tyr Gly Asp Val Gly Leu Ser Lys Gln Thr Leu Phe
260 265 270
Val Tyr Met Gly Thr Asp Pro Ala Asn Asp Asn Asn Thr Phe Thr Asp
275 280 285
Lys Asn Ser Leu Gly Thr Pro Arg Lys Ala Val Ser Gln Arg Asp Ala
290 295 300
Asp Leu Ile His Tyr Trp Glu Lys Tyr Arg Arg Ala Pro Glu Gly Ser
305 310 315 320
Ser Arg Lys Ala Glu Ala Lys Lys Gln Leu Arg Glu Val Met Ala His
325 330 335
Arg Met His Ile Asp Asn
340
<210> 2
<211> 482
<212> PRT
<213> 蝶豆
<400> 2
Met Lys Asn Pro Leu Ala Ile Leu Phe Leu Ile Ala Thr Val Val Ala
1 5 10 15
Val Val Ser Gly Ile Arg Asp Asp Phe Leu Arg Leu Pro Ser Gln Ala
20 25 30
Ser Lys Phe Phe Gln Ala Asp Asp Asn Val Glu Gly Thr Arg Trp Ala
35 40 45
Val Leu Val Ala Gly Ser Lys Gly Tyr Val Asn Tyr Arg His Gln Ala
50 55 60
Asp Val Cys His Ala Tyr Gln Ile Leu Lys Lys Gly Gly Leu Lys Asp
65 70 75 80
Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Ser
85 90 95
Asn Pro His Pro Gly Val Ile Ile Asn His Pro Tyr Gly Ser Asp Val
100 105 110
Tyr Lys Gly Val Pro Lys Asp Tyr Val Gly Glu Asp Ile Asn Pro Pro
115 120 125
Asn Phe Tyr Ala Val Leu Leu Ala Asn Lys Ser Ala Leu Thr Gly Thr
130 135 140
Gly Ser Gly Lys Val Leu Asp Ser Gly Pro Asn Asp His Val Phe Ile
145 150 155 160
Tyr Tyr Thr Asp His Gly Gly Ala Gly Val Leu Gly Met Pro Ser Lys
165 170 175
Pro Tyr Ile Ala Ala Ser Asp Leu Asn Asp Val Leu Lys Lys Lys His
180 185 190
Ala Ser Gly Thr Tyr Lys Ser Ile Val Phe Tyr Val Glu Ser Cys Glu
195 200 205
Ser Gly Ser Met Phe Asp Gly Leu Leu Pro Glu Asp His Asn Ile Tyr
210 215 220
Val Met Gly Ala Ser Asp Thr Gly Glu Ser Ser Trp Val Thr Tyr Cys
225 230 235 240
Pro Leu Gln His Pro Ser Pro Pro Pro Glu Tyr Asp Val Cys Val Gly
245 250 255
Asp Leu Phe Ser Val Ala Trp Leu Glu Asp Cys Asp Val His Asn Leu
260 265 270
Gln Thr Glu Thr Phe Gln Gln Gln Tyr Glu Val Val Lys Asn Lys Thr
275 280 285
Ile Val Ala Leu Ile Glu Asp Gly Thr His Val Val Gln Tyr Gly Asp
290 295 300
Val Gly Leu Ser Lys Gln Thr Leu Phe Val Tyr Met Gly Thr Asp Pro
305 310 315 320
Ala Asn Asp Asn Asn Thr Phe Thr Asp Lys Asn Ser Leu Gly Thr Pro
325 330 335
Arg Lys Ala Val Ser Gln Arg Asp Ala Asp Leu Ile His Tyr Trp Glu
340 345 350
Lys Tyr Arg Arg Ala Pro Glu Gly Ser Ser Arg Lys Ala Glu Ala Lys
355 360 365
Lys Gln Leu Arg Glu Val Met Ala His Arg Met His Ile Asp Asn Ser
370 375 380
Val Lys His Ile Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly His Lys
385 390 395 400
Met Leu Asn Asn Val Arg Pro Ala Gly Leu Pro Val Val Asp Asp Trp
405 410 415
Asp Cys Phe Lys Thr Leu Ile Arg Thr Phe Glu Thr His Cys Gly Ser
420 425 430
Leu Ser Glu Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Leu Cys
435 440 445
Asn Ala Gly Ile Arg Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala
450 455 460
Cys Val Ser Ile Pro Asp Asn Pro Trp Ser Ser Leu His Ala Gly Phe
465 470 475 480
Ser Val
<210> 3
<211> 488
<212> PRT
<213> 蝶豆
<400> 3
Met Ala Val Asp His Cys Phe Leu Lys Lys Lys Thr Cys Tyr Tyr Gly
1 5 10 15
Phe Val Leu Trp Ser Trp Met Leu Met Met Ser Leu His Ser Lys Ala
20 25 30
Ala Arg Leu Asn Pro Gln Lys Glu Trp Asp Ser Val Ile Arg Leu Pro
35 40 45
Thr Glu Pro Val Asp Ala Asp Thr Asp Glu Val Gly Thr Arg Trp Ala
50 55 60
Val Leu Val Ala Gly Ser Asn Gly Tyr Glu Asn Tyr Arg His Gln Ala
65 70 75 80
Asp Val Cys His Ala Tyr Gln Leu Leu Ile Lys Gly Gly Leu Lys Glu
85 90 95
Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Trp His Glu Leu
100 105 110
Asn Pro Arg Pro Gly Val Ile Ile Asn Asn Pro Arg Gly Glu Asp Val
115 120 125
Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Ala Glu
130 135 140
Asn Leu Phe Ala Val Ile Leu Gly Asp Arg Ser Lys Val Lys Gly Gly
145 150 155 160
Ser Gly Lys Val Ile Asn Ser Lys Pro Glu Asp Arg Ile Phe Ile Phe
165 170 175
Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Asn Glu Gln
180 185 190
Ile Leu Tyr Ala Met Asp Phe Ile Asp Val Leu Lys Lys Lys His Ala
195 200 205
Ser Gly Gly Tyr Arg Glu Met Val Ile Tyr Val Glu Ala Cys Glu Ser
210 215 220
Gly Ser Leu Phe Glu Gly Ile Met Pro Lys Asp Leu Asn Val Phe Val
225 230 235 240
Thr Thr Ala Ser Asn Ala Gln Glu Asn Ser Trp Gly Thr Tyr Cys Pro
245 250 255
Gly Thr Glu Pro Ser Pro Pro Pro Glu Tyr Thr Thr Cys Leu Gly Asp
260 265 270
Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Glu Ser His Asn Leu Arg
275 280 285
Arg Glu Thr Val Asn Gln Gln Tyr Arg Ser Val Lys Glu Arg Thr Ser
290 295 300
Asn Phe Lys Asp Tyr Ala Met Gly Ser His Val Met Gln Tyr Gly Asp
305 310 315 320
Thr Asn Ile Thr Ala Glu Lys Leu Tyr Leu Phe Gln Gly Phe Asp Pro
325 330 335
Ala Thr Val Asn Leu Pro Pro His Asn Gly Arg Ile Glu Ala Lys Met
340 345 350
Glu Val Val His Gln Arg Asp Ala Glu Leu Leu Phe Met Trp Gln Met
355 360 365
Tyr Gln Arg Ser Asn His Leu Leu Gly Lys Lys Thr His Ile Leu Lys
370 375 380
Gln Ile Ala Glu Thr Val Lys His Arg Asn His Leu Asp Gly Ser Val
385 390 395 400
Glu Leu Ile Gly Val Leu Leu Tyr Gly Pro Gly Lys Gly Ser Pro Val
405 410 415
Leu Gln Ser Val Arg Asp Pro Gly Leu Pro Leu Val Asp Asn Trp Ala
420 425 430
Cys Leu Lys Ser Met Val Arg Val Phe Glu Ser His Cys Gly Ser Leu
435 440 445
Thr Gln Tyr Gly Met Lys His Met Arg Ala Phe Ala Asn Ile Cys Asn
450 455 460
Ser Gly Val Ser Glu Ser Ser Met Glu Glu Ala Cys Met Val Ala Cys
465 470 475 480
Gly Gly His Asp Ala Gly His Leu
485
<210> 4
<211> 359
<212> PRT
<213> 蝶豆
<400> 4
Glu Asp Val Asn Ala His Asn Phe Phe Ala Val Leu Leu Gly Asn Lys
1 5 10 15
Ser Ala Leu Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asp
20 25 30
Asp His Ile Phe Ile Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu
35 40 45
Gly Met Pro Thr His Pro Tyr Leu Tyr Ala Asp Asp Leu Asn Glu Val
50 55 60
Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Arg Leu Val Phe Tyr
65 70 75 80
Ile Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu
85 90 95
Asp Ile Asp Ile Tyr Ala Thr Thr Ala Ser Asn Ala Thr Glu Ser Ser
100 105 110
Ser Pro Thr Tyr Cys Pro Arg Pro Pro Ala Glu His Ala Pro Phe Pro
115 120 125
Glu Tyr Thr Thr Cys Leu Gly Asp Leu Tyr Ser Ile Thr Trp Met Glu
130 135 140
Asp Ser Asp Ile His Asn Leu Arg Thr Glu Thr Leu His Gln Gln Tyr
145 150 155 160
Glu Leu Val Lys Gly Ile Thr Phe Ala Asp Ser Glu Gly Ser His Val
165 170 175
Met Gln Tyr Gly Asp Ile Asp Leu Ser Ser Asp Val Leu Phe Gln Tyr
180 185 190
Leu Gly Thr Asn Pro Ala Asn Asp Asn Phe Thr Phe Val Asp Glu Asn
195 200 205
Tyr Leu Arg Ser Ser Ser Lys Pro Val Asn Gln His Asp Ala Asp Leu
210 215 220
Ile His Phe Trp Asp Lys Phe Arg Lys Ala Pro Gly Asp Ser Ala Lys
225 230 235 240
Lys Asn Thr Ala Gln Lys Gln Leu Leu Glu Val Met Ser His Arg Met
245 250 255
His Ile Asp Asn Thr Val Gln Leu Ile Gly Lys Leu Leu Phe Gly Ile
260 265 270
Glu Lys Gly Pro Glu Ile Leu Asn Asn Val Arg Pro Val Gly Ser Val
275 280 285
Leu Val Asp Asp Trp Ala Cys Met Lys Ala Met Val Arg Thr Phe Glu
290 295 300
Thr His Cys Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser
305 310 315 320
Phe Ala Asn Ile Cys Asn Ala Gly Ile Lys Asn Glu Gln Met Ala Glu
325 330 335
Ala Ser Gly Gln Ala Cys Val Ser Ile Pro Ala Asn Pro Trp Ser Ser
340 345 350
Leu Gln Arg Gly Phe Ser Ala
355
<210> 5
<211> 193
<212> PRT
<213> 蝶豆
<400> 5
Met Asp Thr Phe Pro Pro Leu Leu Leu Cys Leu Phe Val Leu Ala Ala
1 5 10 15
Val Val Ser Ala Arg Arg Gly Leu Ala Gly Glu Phe Arg Arg Leu Ala
20 25 30
Ser Glu Pro Asp Ile Asp His Asn Phe Arg Gly Thr Lys Trp Ala Val
35 40 45
Leu Leu Ala Gly Ser Arg Gly Phe Phe Asp Tyr Arg His Gln Ala Asp
50 55 60
Val Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Glu Glu
65 70 75 80
Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Asn Asn Glu Glu Asn
85 90 95
Pro Arg Pro Gly Val Ile Ile Asn Ser Pro His Gly Asp Asn Val Tyr
100 105 110
Lys Gly Val Pro Lys Asp Tyr Val Gly Glu Asp Val Asn Ala Asp Asn
115 120 125
Phe Phe Ala Ala Ile Leu Gly Asn Lys Ser Ala Leu Thr Gly Gly Ser
130 135 140
Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr
145 150 155 160
Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Ser Tyr
165 170 175
Val Phe Ala Ser Asp Leu Ile Glu Val Leu Lys Lys Lys His Ala Ser
180 185 190
Gly
<210> 6
<211> 150
<212> PRT
<213> 蝶豆
<400> 6
Met Asp Thr Phe Pro Pro Leu Leu Leu Cys Leu Phe Val Leu Ala Ala
1 5 10 15
Val Val Ser Ala Arg Arg Gly Leu Ala Gly Glu Phe Arg Arg Leu Ala
20 25 30
Ser Glu Pro Asp Ile Asp His Asn Phe Arg Gly Thr Lys Trp Ala Val
35 40 45
Leu Leu Ala Gly Ser Arg Gly Phe Phe Asp Tyr Arg His Gln Ala Asp
50 55 60
Val Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Glu Glu
65 70 75 80
Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Phe Asn Lys Lys Asn
85 90 95
Pro Arg Pro Gly Val Ile Ile Asn Lys Pro Asp Gly Gly Asp Val Tyr
100 105 110
Lys Gly Val Pro Lys Asp Tyr Thr Gly Glu Asn Val Asn Val Asn Asn
115 120 125
Phe Leu Ala Val Leu Leu Gly Asn Lys Ser Ala Leu Thr Gly Gly Ser
130 135 140
Gly Lys Val Leu Asn Ser
145 150
<210> 7
<211> 86
<212> PRT
<213> 蝶豆
<400> 7
Ala Asp Leu Ile His Phe Trp Glu Lys Phe Arg Arg Ala Thr Lys Gly
1 5 10 15
Ser Pro Arg Lys Ala Glu Ala Glu Lys Gln Leu Arg Glu Val Thr Ser
20 25 30
His Arg Met His Ile Asp His Ser Val Lys His Ile Gly Lys Leu Leu
35 40 45
Phe Gly Ile Glu Lys Gly Ser Lys Met Leu Asn Ser Val Arg Pro Ala
50 55 60
Gly Leu Pro Ile Val Asp Asp Trp Asp Cys Leu Lys Thr Met Val Arg
65 70 75 80
Thr Phe Glu Thr His Cys
85
<210> 8
<211> 473
<212> PRT
<213> Oldenlandia affinis
<400> 8
Met Val Arg Tyr Leu Ala Gly Ala Val Leu Leu Leu Val Val Leu Ser
1 5 10 15
Val Ala Ala Ala Val Ser Gly Ala Arg Asp Gly Asp Tyr Leu His Leu
20 25 30
Pro Ser Glu Val Ser Arg Phe Phe Arg Pro Gln Glu Thr Asn Asp His
35 40 45
Gly Glu Asp Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser
50 55 60
Lys Gly Tyr Ala Asn Tyr Arg His Gln Ala Gly Val Cys His Ala Tyr
65 70 75 80
Gln Ile Leu Lys Arg Gly Gly Leu Lys Asp Glu Asn Ile Val Val Phe
85 90 95
Met Tyr Asp Asp Ile Ala Tyr Asn Glu Ser Asn Pro Arg Pro Gly Val
100 105 110
Ile Ile Asn Ser Pro His Gly Ser Asp Val Tyr Ala Gly Val Pro Lys
115 120 125
Asp Tyr Thr Gly Glu Glu Val Asn Ala Lys Asn Phe Leu Ala Ala Ile
130 135 140
Leu Gly Asn Lys Ser Ala Ile Thr Gly Gly Ser Gly Lys Val Val Asp
145 150 155 160
Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His Gly Ala
165 170 175
Ala Gly Val Ile Gly Met Pro Ser Lys Pro Tyr Leu Tyr Ala Asp Glu
180 185 190
Leu Asn Asp Ala Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser
195 200 205
Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Met Phe Glu Gly
210 215 220
Ile Leu Pro Glu Asp Leu Asn Ile Tyr Ala Leu Thr Ser Thr Asn Thr
225 230 235 240
Thr Glu Ser Ser Trp Cys Tyr Tyr Cys Pro Ala Gln Glu Asn Pro Pro
245 250 255
Pro Pro Glu Tyr Asn Val Cys Leu Gly Asp Leu Phe Ser Val Ala Trp
260 265 270
Leu Glu Asp Ser Asp Val Gln Asn Ser Trp Tyr Glu Thr Leu Asn Gln
275 280 285
Gln Tyr His His Val Asp Lys Arg Ile Ser His Ala Ser His Ala Thr
290 295 300
Gln Tyr Gly Asn Leu Lys Leu Gly Glu Glu Gly Leu Phe Val Tyr Met
305 310 315 320
Gly Ser Asn Pro Ala Asn Asp Asn Tyr Thr Ser Leu Asp Gly Asn Ala
325 330 335
Leu Thr Pro Ser Ser Ile Val Val Asn Gln Arg Asp Ala Asp Leu Leu
340 345 350
His Leu Trp Glu Lys Phe Arg Lys Ala Pro Glu Gly Ser Ala Arg Lys
355 360 365
Glu Glu Ala Gln Thr Gln Ile Phe Lys Ala Met Ser His Arg Val His
370 375 380
Ile Asp Ser Ser Ile Lys Leu Ile Gly Lys Leu Leu Phe Gly Ile Glu
385 390 395 400
Lys Cys Thr Glu Ile Leu Asn Ala Val Arg Pro Ala Gly Gln Pro Leu
405 410 415
Val Asp Asp Trp Ala Cys Leu Arg Ser Leu Val Gly Thr Phe Glu Thr
420 425 430
His Cys Gly Ser Leu Ser Glu Tyr Gly Met Arg His Thr Arg Thr Ile
435 440 445
Ala Asn Ile Cys Asn Ala Gly Ile Ser Glu Glu Gln Met Ala Glu Ala
450 455 460
Ala Ser Gln Ala Cys Ala Ser Ile Pro
465 470
<210> 9
<211> 486
<212> PRT
<213> Hedyotis biflora
<400> 9
Met Val Arg Tyr Pro Ala Gly Val Val Leu Leu Leu Val Thr Ile Thr
1 5 10 15
Ile Ser Val Val Ala Glu Ala Arg Asp Gly Tyr Leu Lys Leu Pro Ser
20 25 30
Glu Phe Ser Ala Phe Leu Arg Pro Asn Glu Thr Asn Asp Asn Ser Val
35 40 45
Ser Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Lys Asp Tyr Trp Asn
50 55 60
Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Ile Leu Lys Arg
65 70 75 80
Gly Gly Leu Lys Asp Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile
85 90 95
Ala Tyr Asn Glu Glu Asn Pro Arg Pro Gly Val Ile Ile Asn Ser Pro
100 105 110
His Gly Ser Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Asp
115 120 125
Glu Val Asn Ala Lys Asn Phe Leu Ala Ala Ile Leu Gly Asp Lys Ser
130 135 140
Ala Ile Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp
145 150 155 160
His Ile Phe Ile Tyr Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly
165 170 175
Met Pro Ser Gly Pro Tyr Leu Tyr Ala Asp Glu Leu Asn Asp Ala Leu
180 185 190
Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu
195 200 205
Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly
210 215 220
Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp
225 230 235 240
Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Ser Glu Tyr Glu
245 250 255
Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Glu
260 265 270
Val His Asn Leu Arg Ser Glu Thr Leu Lys Gln Gln Tyr His Leu Val
275 280 285
Lys Thr Arg Thr Ser Asn Gly Asn Ser Ala Tyr Gly Ser His Val Met
290 295 300
Gln Tyr Gly Asp Leu Lys Leu Ser Val Asp Asn Leu Phe Leu Tyr Met
305 310 315 320
Gly Thr Asn Pro Ala Asn Asp Asn Tyr Thr Phe Val Asp Asp Asn Ala
325 330 335
Leu Arg Pro Ser Ser Lys Ala Ile Asn Gln Arg Asp Ala Asp Leu Val
340 345 350
His Phe Trp Asp Lys Phe Arg Lys Ala Pro Glu Gly Ser Leu Arg Lys
355 360 365
Glu Glu Ala Ala Lys Gln Val Phe Glu Ala Met Ser His Arg Met His
370 375 380
Ile Asp Ser Ser Ile Lys Leu Val Gly Lys Leu Leu Phe Gly Ile Glu
385 390 395 400
Arg Gly Ser Glu Ile Leu Asn Ala Val Arg Pro Ala Gly Gln Pro Leu
405 410 415
Ala Asp Asp Trp Ala Cys Leu Lys Ser Leu Val Arg Thr Phe Glu Thr
420 425 430
His Cys Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Thr Val
435 440 445
Ala Asn Ile Cys Asn Ala Gly Ile Thr Lys Glu Gln Met Ala Glu Ala
450 455 460
Ala Ala Gln Ala Cys Val Ser Val Pro Ser Asn Pro Trp Ser Ser Leu
465 470 475 480
Ser Gly Gly Phe Ser Ala
485
<210> 10
<211> 483
<212> PRT
<213> Phaseolus vulgaris
<400> 10
Met Asp Gly Phe Pro Ile Leu Phe Leu Leu Ala Thr Leu Ile Thr Leu
1 5 10 15
Val Ser Gly Gly Arg Asp Glu Ile Leu Arg Leu Pro Ser Glu Ala Ser
20 25 30
Arg Phe Phe Gln Ala Pro Ala Ala Asp Gln Asn Gln Glu Gly Thr Arg
35 40 45
Trp Ala Leu Leu Ile Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His
50 55 60
Gln Ser Asp Val Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu
65 70 75 80
Lys Glu Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Phe Asn
85 90 95
Glu Glu Asn Pro Arg Pro Gly Val Ile Ile Asn Ser Pro His Gly Asn
100 105 110
Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Val Gly Glu Asp Val Thr
115 120 125
Val Asn Asn Phe Phe Ala Ala Ile Leu Gly Asn Lys Ser Ala Leu Thr
130 135 140
Gly Gly Ser Gly Lys Val Ile Asp Ser Gly Pro Asn Asp His Ile Phe
145 150 155 160
Ile Tyr Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr
165 170 175
Ser Pro Tyr Met Tyr Ala Ser Asp Leu Ile Glu Val Leu Lys Lys Lys
180 185 190
His Ala Ser Glu Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys
195 200 205
Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly Met Asn Ile
210 215 220
Tyr Ala Thr Thr Ala Ala Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr
225 230 235 240
Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu
245 250 255
Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Ile His Asn
260 265 270
Leu Gln Thr Glu Thr Leu His Gln Gln Phe Glu Leu Val Lys Gln Arg
275 280 285
Thr Ile Asn Gly Asn Ser Ala Tyr Gly Ser His Val Met Gln Tyr Gly
290 295 300
Asp Ile Gly Leu Ser Lys Asn Asn Leu Ser Leu Tyr Leu Gly Thr Asn
305 310 315 320
Pro Ala Asn Asp Asn Phe Ala Phe Arg Glu Lys Asn Ser Leu Val Pro
325 330 335
Pro Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val His Leu Trp
340 345 350
Asp Lys Tyr Arg Lys Ala Pro Val Gly Ser Ser Arg Lys Ser Val Ala
355 360 365
Gln Lys Gln Ile Leu Glu Ala Met Ser His Arg Met His Ile Asp Asp
370 375 380
Ser Met Lys Leu Ile Gly Lys Leu Leu Phe Gly Ile Glu Glu Gly Pro
385 390 395 400
Lys Leu Leu Asn Ser Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp
405 410 415
Trp Asp Cys Leu Lys Thr Leu Val Arg Thr Phe Glu Thr His Cys Gly
420 425 430
Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Phe
435 440 445
Cys Asn Ala Gly Ile Gly Lys Glu Gln Met Ala Glu Ala Ser Ala Gln
450 455 460
Ala Cys Val Ser Ile Pro Ala Thr Pro Trp Ser Ser Leu Arg Ser Gly
465 470 475 480
Phe Ser Ala
<210> 11
<211> 484
<212> PRT
<213> Glycine max
<400> 11
Met Asp Arg Phe Pro Ile Leu Phe Leu Val Ala Thr Leu Ile Thr Leu
1 5 10 15
Ala Ser Gly Ala Arg His Asp Ile Leu Arg Leu Pro Ser Glu Ala Ser
20 25 30
Arg Phe Phe Lys Ala Pro Ala Asn Ala Asp Gln Asn Asp Glu Gly Thr
35 40 45
Arg Trp Ala Val Leu Val Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg
50 55 60
His Gln Ser Asp Val Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly
65 70 75 80
Val Lys Glu Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Phe
85 90 95
Asn Glu Glu Asn Pro Arg Pro Gly Val Ile Ile Asn Ser Pro His Gly
100 105 110
Asn Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Val Gly Glu Asp Val
115 120 125
Thr Val Asp Asn Phe Phe Ala Ala Ile Leu Gly Asn Lys Ser Ala Leu
130 135 140
Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile
145 150 155 160
Phe Ile Tyr Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro
165 170 175
Thr Asn Pro Tyr Met Tyr Ala Ser Asp Leu Ile Glu Val Leu Lys Lys
180 185 190
Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala
195 200 205
Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn
210 215 220
Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr
225 230 235 240
Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Glu Thr Cys
245 250 255
Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Ile His
260 265 270
Asn Leu Arg Thr Glu Thr Leu His Gln Gln Tyr Asp Leu Val Lys Glu
275 280 285
Arg Thr Met Asn Gly Asn Ser Ile Tyr Gly Ser His Val Met Gln Tyr
290 295 300
Gly Asp Ile Gly Leu Ser Lys Asn Asn Leu Val Leu Tyr Leu Gly Thr
305 310 315 320
Asn Pro Ala Asn Asp Asn Phe Thr Phe Val His Lys Asn Ser Leu Val
325 330 335
Pro Pro Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Ile His Phe
340 345 350
Trp Asp Lys Phe Arg Lys Ala Pro Val Gly Ser Ser Arg Lys Ala Ala
355 360 365
Ala Glu Lys Glu Ile Leu Glu Ala Met Ser His Arg Met His Ile Asp
370 375 380
Asp Asn Met Lys Leu Ile Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly
385 390 395 400
Pro Glu Leu Leu Ser Ser Val Arg Pro Ala Gly Gln Pro Leu Val Asp
405 410 415
Asp Trp Asp Cys Leu Lys Thr Leu Val Arg Thr Phe Glu Thr His Cys
420 425 430
Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn
435 440 445
Phe Cys Asn Ala Gly Ile Arg Lys Glu Gln Met Ala Glu Ala Ser Ala
450 455 460
Gln Ala Cys Val Ser Ile Pro Ala Ser Ser Trp Ser Ser Leu His Arg
465 470 475 480
Gly Phe Ser Ala
<210> 12
<211> 483
<212> PRT
<213> 人
<400> 12
Met Asp Arg Phe Pro Ile Leu Phe Leu Leu Ala Thr Leu Ile Thr Leu
1 5 10 15
Ala Ser Gly Ala Arg His Asp Ile Leu Arg Leu Pro Ser Glu Ala Ser
20 25 30
Thr Phe Phe Lys Ala Pro Gly Gly Asp Gln Asn Asp Glu Gly Thr Arg
35 40 45
Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His
50 55 60
Gln Ser Asp Val Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu
65 70 75 80
Lys Glu Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Phe Asn
85 90 95
Glu Glu Asn Pro Arg Pro Gly Val Ile Ile Asn Ser Pro His Gly Asn
100 105 110
Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Ile Gly Glu Asp Val Thr
115 120 125
Val Gly Asn Phe Phe Ala Ala Ile Leu Gly Asn Lys Ser Ala Leu Thr
130 135 140
Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe
145 150 155 160
Ile Tyr Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr
165 170 175
Asn Pro Tyr Val Tyr Ala Ser Asp Leu Ile Glu Val Leu Lys Lys Lys
180 185 190
His Ala Ser Gly Ser Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys
195 200 205
Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile
210 215 220
Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr
225 230 235 240
Cys Pro Gly Glu Tyr Pro Ser Pro Pro Ser Glu Tyr Glu Thr Cys Leu
245 250 255
Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Ile His Asn
260 265 270
Leu Gln Thr Glu Thr Leu His Gln Gln Tyr Glu Leu Val Lys Gln Arg
275 280 285
Thr Met Asn Gly Asn Ser Ile Tyr Gly Ser His Val Met Gln Tyr Gly
290 295 300
Asp Ile Gly Leu Ser Glu Asn Asn Leu Val Leu Tyr Leu Gly Thr Asn
305 310 315 320
Pro Ala Asn Asp Asn Phe Thr Phe Val Leu Lys Asn Ser Leu Val Pro
325 330 335
Pro Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Ile His Phe Trp
340 345 350
Asp Lys Phe Arg Lys Ala Pro Val Gly Ser Ser Arg Lys Ala Ala Ala
355 360 365
Glu Lys Gln Ile Leu Glu Ala Met Ser His Arg Met His Ile Asp Asp
370 375 380
Ser Met Lys Arg Ile Gly Lys Leu Phe Phe Gly Ile Glu Lys Gly Pro
385 390 395 400
Glu Leu Leu Ser Ser Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp
405 410 415
Trp Asp Cys Leu Lys Thr Leu Val Arg Thr Phe Glu Thr His Cys Gly
420 425 430
Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Phe
435 440 445
Cys Asn Ala Gly Ile Arg Lys Glu Gln Met Ala Glu Ala Ser Ala Gln
450 455 460
Ala Cys Val Asn Ile Pro Ala Ser Ser Trp Ser Ser Met His Arg Gly
465 470 475 480
Phe Ser Ala
<210> 13
<211> 483
<212> PRT
<213> Vigna mungo
<400> 13
Met Asn Arg Phe Pro Ile Ile Phe Val Val Ala Asn Leu Ile Thr Leu
1 5 10 15
Val Ser Gly Gly Arg Asp Glu Ile Leu Arg Met Pro Ser Glu Ala Ser
20 25 30
Arg Phe Phe Gln Ala Pro Ala Thr Asp Glu Asn Asp Glu Gly Thr Arg
35 40 45
Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His
50 55 60
Gln Ser Asp Val Cys His Ala Tyr Gln Leu Leu Thr Lys Gly Gly Leu
65 70 75 80
Lys Glu Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Phe Asn
85 90 95
Glu Glu Asn Pro Arg Pro Gly Val Ile Ile Asn Ser Pro His Gly Asn
100 105 110
Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Val Gly Glu Asp Val Thr
115 120 125
Val Asn Asn Phe Phe Ala Ala Ile Leu Gly Asn Lys Ser Ala Leu Thr
130 135 140
Gly Gly Ser Gly Lys Val Val Asn Ser Gly Pro Asn Asp His Ile Phe
145 150 155 160
Ile Tyr Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr
165 170 175
Ser Pro Tyr Met Tyr Ala Ser Asp Leu Ile Glu Val Leu Lys Lys Lys
180 185 190
His Ala Ser Gly Thr Tyr Lys Ser Leu Ala Phe Tyr Leu Glu Gly Cys
195 200 205
Glu Ser Gly Ser Ile Phe Gly Gly Leu Leu Pro Glu Gly Leu Asn Ile
210 215 220
Tyr Ala Thr Thr Ala Ala Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr
225 230 235 240
Cys Pro Gly Asp Asn Pro Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu
245 250 255
Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Ile His Asn
260 265 270
Leu Arg Thr Glu Thr Leu His Gln Gln Phe Glu Leu Val Lys Gln Arg
275 280 285
Thr Met Asn Gly Asn Ser Ala Tyr Gly Ser His Val Met Gln Tyr Gly
290 295 300
Asp Val Gly Leu Ser Lys Asn Asn Val Ser Leu Tyr Leu Gly Thr Asn
305 310 315 320
Pro Ala Asn Asp Asn Phe Pro Phe Arg Glu Lys Asn Ser Leu Val Pro
325 330 335
Pro Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp
340 345 350
Asp Lys Phe Pro Lys Ala Pro Leu Gly Ser Ser Arg Lys Ser Val Ala
355 360 365
Gln Lys Gln Ile Leu Glu Ala Met Ser His Arg Met His Ile Asp Asp
370 375 380
Ser Val Thr Leu Ile Gly Lys Leu Leu Phe Gly Ile Glu Glu Gly Pro
385 390 395 400
Glu Leu Leu Ser Ser Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp
405 410 415
Trp Asp Cys Leu Lys Thr Leu Val Arg Thr Phe Glu Thr His Cys Gly
420 425 430
Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Leu
435 440 445
Cys Asn Ala Gly Ile Arg Lys Glu Gln Met Ala Glu Ala Ser Ala Gln
450 455 460
Ala Cys Val Ser Ile Pro Ala Thr Pro Trp Ser Ser Leu Ser Ser Gly
465 470 475 480
Phe Ser Ala
<210> 14
<211> 488
<212> PRT
<213> Cicer arietinum
<400> 14
Met Tyr Arg Phe Pro Thr Pro Thr Leu Leu Phe Leu Ile Val Thr Leu
1 5 10 15
Ile Ala Leu Val Ser Ser Asn Pro Glu Asp Phe Leu Arg Leu Pro Ser
20 25 30
Glu Ser Ser Arg Phe Phe His Ser Pro Ser Ala Asp Asp Lys Glu Asn
35 40 45
Asn Glu Gly Thr Arg Trp Ala Ile Leu Ile Ala Gly Ser Asn Gly Tyr
50 55 60
Trp Asn Tyr Arg His Gln Ser Asp Val Cys His Ala Tyr Gln Val Leu
65 70 75 80
Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile Val Val Phe Met Tyr Asp
85 90 95
Asp Ile Ala Phe Asn Glu Glu Asn Pro Arg Pro Gly Val Ile Ile Asn
100 105 110
Ser Pro His Gly Asp Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Thr
115 120 125
Gly Glu Asp Val Asn Val Asp Asn Phe Phe Ala Ala Leu Leu Gly Asn
130 135 140
Lys Ser Ala Leu Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro
145 150 155 160
Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His Gly Gly Pro Gly Val
165 170 175
Leu Gly Met Pro Thr Ser Pro Tyr Met Tyr Ala Ser Asp Leu Ile Glu
180 185 190
Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe
195 200 205
Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro
210 215 220
Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ala Asn Ala Asp Glu Ser
225 230 235 240
Ser Trp Gly Thr Tyr Cys Pro Gly Glu Phe Pro Ser Pro Pro Pro Glu
245 250 255
Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp
260 265 270
Ser Asp Met His Asn Leu Gln Ser Glu Thr Leu His Gln Gln Tyr Glu
275 280 285
Leu Val Lys Glu Arg Thr Lys Asn Gly Asn Thr Leu Tyr Gly Ser His
290 295 300
Val Met Gln Tyr Gly Asp Ile Gly Leu Ser Glu Asn Ser Leu Phe Leu
305 310 315 320
Tyr Leu Gly Thr Asn Pro Ala Asn Glu Asn Phe Thr Phe Val Gly Arg
325 330 335
Asn Ser Leu Val Pro Pro Ser Lys Ala Val Asn Gln Arg Asp Ala Asp
340 345 350
Leu Val His Phe Trp Asp Lys Phe Arg Lys Ala Pro Gln Gly Ser Pro
355 360 365
Arg Lys Ala Ala Ala Glu Lys Gln Val Leu Glu Ala Met Ser His Arg
370 375 380
Met His Ile Asp Asp Ser Ile Lys Leu Val Gly Lys Leu Leu Phe Gly
385 390 395 400
Met Glu Lys Gly Pro Glu Val Leu Thr Ser Val Arg Pro Ala Gly Gln
405 410 415
Pro Leu Ala Asp Asp Trp Asn Cys Leu Lys Thr Leu Val Arg Thr Phe
420 425 430
Glu Thr Tyr Cys Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg
435 440 445
Ser Phe Ala Asn Phe Cys Asn Ala Gly Ile His Lys Glu Gln Met Ala
450 455 460
Glu Ala Ser Ala Gln Ala Cys Val Asn Val Pro Ala Asn Pro Trp Ser
465 470 475 480
Ser Leu Arg Ser Gly Phe Ser Ala
485
<210> 15
<211> 492
<212> PRT
<213> Ricinus communis
<400> 15
Met Thr Ile Arg Leu Ser Thr Gly Ile Ile Leu Ile Leu Leu Thr Leu
1 5 10 15
Cys Gly Val Val Ser Ser Ser Arg Asp Ile Val Gly Asp Val Ile Arg
20 25 30
Leu Pro Ser Glu Ala Ser Arg Phe Phe Arg Pro Ala Asp Gly Lys Asn
35 40 45
Gly Asp Asp Asp Ser Ala Gly Thr Arg Trp Ala Ile Leu Ile Ala Gly
50 55 60
Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His Ala
65 70 75 80
Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile Ile Val
85 90 95
Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Glu Asn Pro Arg Gln Gly
100 105 110
Ile Ile Ile Asn Asn Pro His Gly Glu Asp Val Tyr Lys Gly Val Pro
115 120 125
Lys Asp Tyr Thr Gly Glu Asn Val Thr Val Gly Asn Phe Phe Ala Ala
130 135 140
Ile Leu Gly Asn Arg Thr Ala Leu Thr Gly Gly Arg Gly Lys Val Val
145 150 155 160
Asp Ser Gly Pro Asn Asp His Ile Phe Val Tyr Tyr Thr Asp His Gly
165 170 175
Gly Pro Gly Val Leu Gly Met Pro Thr Asn Pro Tyr Leu Tyr Ala Asn
180 185 190
Asp Leu Ile Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys
195 200 205
Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu
210 215 220
Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn
225 230 235 240
Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser
245 250 255
Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ala
260 265 270
Trp Met Glu Asp Ser Asp Val His Asn Leu Gln Thr Glu Thr Leu His
275 280 285
Gln Gln Tyr Glu Leu Val Lys Arg Arg Thr Ser Asn Gly Asn Ser Ala
290 295 300
Tyr Gly Ser His Val Met Gln Tyr Gly Asp Val Gly Leu Ser Arg Glu
305 310 315 320
Asn Leu Phe Leu Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Tyr Thr
325 330 335
Phe Val Asp Glu Asn Ser Leu Thr Pro Pro Ser Lys Ala Val Asn Gln
340 345 350
Arg Asp Ala Asp Leu Val His Phe Trp Asp Lys Tyr Arg Lys Ala Pro
355 360 365
Asp Gly Ser Ala Arg Lys Asp Gln Ala Gln Lys Gln Phe Val Glu Ala
370 375 380
Met Ser His Arg Met His Ile Asp His Ser Val Lys Leu Ile Gly Lys
385 390 395 400
Leu Leu Phe Gly Leu Glu Lys Ala Ser Glu Val Leu Ser Thr Val Arg
405 410 415
Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Lys Leu
420 425 430
Val Arg Thr Phe Glu Thr His Cys Gly Ser Ile Ser Gln Tyr Gly Met
435 440 445
Lys His Met Arg Ser Leu Ala Asn Leu Cys Asn Ala Gly Ile Arg Glu
450 455 460
Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Ile Thr Phe Pro Ser
465 470 475 480
Gly Pro Trp Ser Ser Leu His Lys Gly Phe Ser Ala
485 490
<210> 16
<211> 487
<212> PRT
<213> Theobroma cacao
<400> 16
Met Thr Arg Leu Val Ser Gly Val Ile Leu Leu Leu Leu Ser Leu Thr
1 5 10 15
Gly Ile Val Ser Ala Gly Arg Asp Ile Thr Gly Asp Val Leu Arg Leu
20 25 30
Pro Ser Glu Ala Ser Lys Phe Phe Arg Gly Ser Asn Asp Asp Glu Val
35 40 45
Glu Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Trp
50 55 60
Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Leu Leu Lys
65 70 75 80
Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp
85 90 95
Ile Ala Phe Asn Glu Glu Asn Pro Arg Pro Gly Ile Ile Ile Asn Ser
100 105 110
Pro His Gly Asp Asp Val Tyr Glu Gly Val Pro Lys Asp Tyr Thr Gly
115 120 125
Glu Asp Val Thr Val Asn Asn Leu Leu Ala Ala Ile Leu Gly Asn Lys
130 135 140
Thr Ala Leu Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn
145 150 155 160
Asp His Ile Phe Ile Tyr Tyr Thr Asp His Gly Gly Pro Gly Val Leu
165 170 175
Gly Met Pro Thr Phe Pro Tyr Leu Tyr Ala Asp Asp Leu Ile Glu Val
180 185 190
Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr
195 200 205
Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu
210 215 220
Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser
225 230 235 240
Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr
245 250 255
Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser
260 265 270
Asp Ile His Asn Leu Gln Thr Glu Thr Leu His Gln Gln Tyr Glu Leu
275 280 285
Val Lys Arg Arg Thr Ile Asn Gly Asn Ser Ala Tyr Gly Ser His Val
290 295 300
Met Gln Tyr Gly Asp Ile Gly Leu Ser Lys Asp Ile Val Phe Val Tyr
305 310 315 320
Leu Gly Thr Asn Pro Ala Asn Asp Asn Phe Thr Phe Val Asp Glu Asn
325 330 335
Ser Leu Gln Pro Pro Thr Lys Ala Val Asn Gln Arg Asp Ala Asp Leu
340 345 350
Val His Phe Trp Asp Lys Tyr Arg Lys Ala Pro Asp Gly Ser Val Arg
355 360 365
Lys Leu Glu Ala Gln Lys Gln Phe Val Glu Ala Met Ser His Arg Met
370 375 380
His Ile Asp Asn Ser Met Lys Leu Ile Gly Lys Leu Leu Phe Gly Ile
385 390 395 400
Glu Lys Gly Pro Glu Val Met Lys Thr Val Arg Pro Ala Gly Gln Pro
405 410 415
Leu Val Asp Asp Trp Lys Cys Leu Lys Lys Met Val Arg Thr Phe Glu
420 425 430
Thr His Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg Ser
435 440 445
Leu Ala Asn Ile Cys Asn Ala Gly Ile Gln Thr Glu Gln Met Ala Glu
450 455 460
Ala Ser Ala Gln Ala Cys Val Ser Ile Pro Ser Gly His Trp Ser Ser
465 470 475 480
Val Gln Lys Gly Phe Ser Ala
485
<210> 17
<211> 484
<212> PRT
<213> Medicago truncatula
<400> 17
Met Ala Arg Phe Leu Phe Leu Ile Ile Ala Thr Leu Ile Pro Ile Phe
1 5 10 15
Ser Ala Ala Thr Ala Thr Ala Gly Asp Asp Phe Leu Arg Leu Pro Ser
20 25 30
Gln Ala Ser Arg Phe Phe Gln Ser Asp Asp Asp Asn Asn Glu Gly Thr
35 40 45
Lys Trp Ala Ile Leu Ile Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg
50 55 60
His Gln Ser Asp Val Cys His Ala Tyr Gln Val Leu Arg Lys Gly Gly
65 70 75 80
Leu Lys Glu Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Asp
85 90 95
Asn Gln Glu Asn Pro Arg Pro Gly Val Ile Ile Asn Ser Pro His Gly
100 105 110
Asp Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Thr Gly Asp Asp Val
115 120 125
Asn Val Asn Asn Phe Phe Ala Ala Leu Leu Gly Asn Lys Ser Ala Leu
130 135 140
Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile
145 150 155 160
Phe Ile Tyr Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro
165 170 175
Thr Gly Pro Phe Met Tyr Ala Thr Asp Leu Ile Glu Val Leu Lys Lys
180 185 190
Lys His Ala Ser Glu Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala
195 200 205
Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn
210 215 220
Ile Tyr Ala Thr Thr Ala Ala Asn Ala Glu Glu Ser Ser Trp Gly Thr
225 230 235 240
Tyr Cys Pro Gly Glu Asn Pro Ser Pro Pro Pro Glu Tyr Glu Thr Cys
245 250 255
Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Ile His
260 265 270
Asn Leu Gln Thr Glu Thr Leu His Gln Gln Tyr Glu Leu Val Lys Glu
275 280 285
Arg Thr Ser Asn Gly Asn Ser Ile Tyr Gly Ser His Val Met Gln Phe
290 295 300
Gly Asp Ile Gly Leu Ser Arg Asp Ser Leu Phe Leu Tyr Leu Gly Ser
305 310 315 320
Asn Pro Ala Asn Glu Asn Phe Thr Phe Met Gly Arg Asn Ser Leu Val
325 330 335
Pro Pro Ser Lys Thr Val Asn Gln Arg Asp Ala Asp Leu Ile His Phe
340 345 350
Trp Asp Lys Phe Arg Lys Ala Pro Gln Gly Ser Pro Arg Lys Val Ala
355 360 365
Ala Gln Lys Gln Val Leu Glu Ala Met Ser His Arg Met His Ile Asp
370 375 380
Glu Ser Ile Lys Leu Val Gly Lys Leu Leu Phe Gly Met Lys Lys Gly
385 390 395 400
Pro Glu Val Leu Ala Ser Val Arg Pro Ala Gly Gln Pro Val Val Asp
405 410 415
Asp Trp Asp Cys Leu Lys Ser Leu Val Arg Thr Phe Glu Thr Tyr Cys
420 425 430
Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn
435 440 445
Phe Cys Asn Ala Gly Ile His Ser Glu Gln Met Ala Glu Ala Ser Ala
450 455 460
Gln Ala Cys Ile Asn Ile Pro Ala Asn Pro Trp Ser Ser Leu His Gly
465 470 475 480
Gly Phe Ser Ala
<210> 18
<211> 493
<212> PRT
<213> Populus tomentosa
<400> 18
Met Thr Arg Leu Ile Ala Gly Val Ile Phe Leu Leu Ile Ala Phe Cys
1 5 10 15
Gly Ile Ala Val Gly Val Arg Asp Ile Val Gly Asp Val Leu Arg Leu
20 25 30
Pro Ser Glu Ala Ser Arg Phe Phe Arg Ser Gly Lys Phe Asn Asp Asp
35 40 45
Asn Ser Asp Asp Asp Ser Ser Gly Thr Arg Trp Ala Ile Leu Leu Ala
50 55 60
Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His
65 70 75 80
Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile Ile
85 90 95
Val Phe Met Tyr Asp Asp Ile Ala Asp Asn Pro Glu Asn Pro Arg Pro
100 105 110
Gly Val Ile Ile Asn Asn Pro Gln Gly Glu Asp Val Tyr Glu Gly Val
115 120 125
Pro Lys Asp Tyr Thr Gly Gln Asp Val Thr Val Gly Asn Phe Phe Ala
130 135 140
Ala Ile Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser Gly Lys Val
145 150 155 160
Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His
165 170 175
Gly Gly Pro Gly Val Leu Gly Met Pro Thr Asn Pro Tyr Leu Tyr Ala
180 185 190
Asp Asp Leu Ile Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr
195 200 205
Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe
210 215 220
Glu Gly Leu Leu Pro Gln Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser
225 230 235 240
Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro
245 250 255
Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val
260 265 270
Ala Trp Met Glu Asp Ser Asp Ile His Asn Leu Arg Thr Glu Thr Leu
275 280 285
His Gln Gln Tyr Glu Leu Val Lys Arg Arg Thr Ser Asp Glu Asn Ser
290 295 300
Ala Tyr Gly Ser His Val Met Gln Tyr Gly Asp Val Gly Leu Ser Lys
305 310 315 320
Glu Asp Leu Phe Gln Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Phe
325 330 335
Thr Phe Leu Glu Asp Asn Ser Leu Arg Pro Pro Ser Lys Ala Val Asn
340 345 350
Gln Arg Asp Ala Asp Leu Val His Phe Trp Ala Lys Tyr Arg Lys Ala
355 360 365
Pro Glu Gly Ser Ser Arg Lys Val Glu Ala Gln Lys Gln Phe Val Glu
370 375 380
Ala Met Ser His Arg Met His Ile Asp His Ser Ile Lys Leu Ile Gly
385 390 395 400
Lys Leu Leu Phe Gly Ile Glu Lys Ala Ser Glu Val Leu Asn Asn Val
405 410 415
Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Val Cys Leu Lys Thr
420 425 430
Leu Val Arg Thr Phe Glu Thr His Cys Gly Ser Ile Ser Gln Tyr Gly
435 440 445
Met Lys His Met Arg Ser Leu Ala Asn Leu Cys Asn Ala Gly Ile Val
450 455 460
Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Val Ser Ile Pro
465 470 475 480
Ser Gly Ser Trp Ser Ser Leu His Lys Gly Phe Ser Ala
485 490
<210> 19
<211> 489
<212> PRT
<213> Populus trichocarpa
<400> 19
Met Thr Gly Leu Ala Thr Gly Ala Ile Phe Leu Leu Ile Ser Leu Cys
1 5 10 15
Gly Ile Ala Ala Ala Gly Arg Asp Thr Val Gly Asp Val Leu Arg Leu
20 25 30
Pro Ser Glu Ala Ser Arg Phe Phe His Asn Asp Asp Asn Ser Asp Asp
35 40 45
Asp Ser Thr Gly Thr Arg Trp Ala Ile Leu Leu Ala Gly Ser Asn Gly
50 55 60
Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Leu
65 70 75 80
Leu Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile Ile Val Phe Met Tyr
85 90 95
Asp Asp Ile Ala Tyr Asn Ser Glu Asn Pro Arg Arg Gly Val Ile Ile
100 105 110
Asn Ser Pro Gln Gly Glu Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr
115 120 125
Thr Gly Glu Asp Val Thr Val Gly Asn Phe Phe Ala Ala Ile Leu Gly
130 135 140
Asn Lys Thr Ala Leu Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly
145 150 155 160
Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His Gly Gly Pro Gly
165 170 175
Val Leu Gly Met Pro Thr Asn Pro Tyr Leu Tyr Ala Asp Asp Leu Ile
180 185 190
Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val
195 200 205
Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu
210 215 220
Pro Gln Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu
225 230 235 240
Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Asn Pro Ser Pro Pro Pro
245 250 255
Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu
260 265 270
Asp Ser Asp Ile His Asn Leu Gln Thr Glu Thr Leu His Gln Gln Tyr
275 280 285
Glu Leu Val Lys Arg Arg Thr Ser Asn Asp Asn Ser Pro Tyr Gly Ser
290 295 300
His Val Met Gln Tyr Gly Asp Val Gly Leu Ser Lys Asp Asn Ile Phe
305 310 315 320
Leu Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Phe Thr Phe Met Asp
325 330 335
Glu Asn Leu Leu Arg Pro Arg Ser Lys Ala Val Asn Gln Arg Asp Ala
340 345 350
Asp Leu Val His Phe Trp Asp Lys Tyr Arg Lys Ala Pro Glu Gly Ser
355 360 365
Ser Arg Lys Val Glu Ala Gln Lys Gln Phe Val Glu Ala Met Ser His
370 375 380
Arg Met His Ile Asp His Ser Ile Lys Leu Ile Gly Lys Leu Leu Phe
385 390 395 400
Gly Ile Glu Lys Ala Ser Glu Val Leu Asn Ala Ile Arg Pro Ala Gly
405 410 415
Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Thr Leu Val Arg Thr
420 425 430
Phe Glu Thr His Cys Gly Ser Val Ser Gln Tyr Gly Met Lys His Met
435 440 445
Arg Ser Leu Ala Asn Leu Cys Asn Ala Gly Ile Gly Lys Glu Gln Met
450 455 460
Ala Glu Ala Ser Ala Gln Ala Cys Val Ser Phe Pro Ser Gly Pro Trp
465 470 475 480
Ser Thr Leu His Lys Gly Phe Ser Ala
485
<210> 20
<211> 494
<212> PRT
<213> Malus hupehensis var. mengshanensis
<220>
<221> misc_feature
<222> (264)..(264)
<223> Xaa可以是任何天然存在的氨基酸
<400> 20
Met Thr Arg Leu Ala Ser Ala Val Val Leu Leu Phe Leu Ala Ser Val
1 5 10 15
Leu Ala Ser Ala Ala Gly Ser Arg Asp Leu Ile Gly Asp Val Leu Arg
20 25 30
Leu Pro Ser Glu Ala Ser Arg Phe Phe Gly Arg Gly Asp Asp Ala Pro
35 40 45
Asp Gln Gln Asp Asp Gly Thr Val Gly Thr Arg Trp Ala Val Leu Ile
50 55 60
Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys
65 70 75 80
His Ala Tyr Gln Leu Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile
85 90 95
Val Val Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Glu Asn Pro Arg
100 105 110
Gln Gly Val Ile Ile Asn Ser Pro His Gly Ser Asp Val Tyr Glu Gly
115 120 125
Val Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val Asn Asn Phe Phe
130 135 140
Ala Ala Ile Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser Gly Lys
145 150 155 160
Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp
165 170 175
His Gly Gly Pro Gly Ile Leu Gly Met Pro Thr Ser Pro Tyr Ile Tyr
180 185 190
Ala Asn Asp Leu Ile Glu Val Leu Lys Lys Lys His Ala Ala Gly Thr
195 200 205
Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile
210 215 220
Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Phe Ala Thr Thr Ala
225 230 235 240
Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr
245 250 255
Pro Ser Pro Pro Pro Glu Tyr Xaa Thr Cys Leu Gly Asp Leu Tyr Ser
260 265 270
Val Val Trp Met Glu Asp Ser Asp Val His Asn Leu Arg Ser Glu Thr
275 280 285
Leu His Gln Gln Tyr Glu Leu Val Lys Met Arg Thr Ala Asn Asp Asn
290 295 300
Ser Gly Phe Gly Ser His Val Met Gln Tyr Gly Asp Val Gly Leu Ser
305 310 315 320
Lys Asn Asn Leu Phe Val Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn
325 330 335
Tyr Thr Phe Leu Gly Glu Asn Ser Leu Arg Pro Ser Ser Lys Ala Val
340 345 350
Asn Gln Arg Asp Ala Asp Leu Leu Arg Phe Trp His Lys Tyr Arg Lys
355 360 365
Ala Pro Glu Gly Ser Ala Arg Lys Ile Gln Ala Gln Lys Asp Phe Val
370 375 380
Glu Ala Met Ser His Arg Met His Ile Asp Gln Thr Met Lys Leu Ile
385 390 395 400
Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly Pro Gln Val Leu Asn Ala
405 410 415
Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys
420 425 430
Thr Met Val Arg Ser Phe Glu Thr His Cys Gly Ser Leu Ser Gln Tyr
435 440 445
Gly Met Lys His Met Arg Ser Leu Ala Asn Ile Cys Asn Ala Gly Met
450 455 460
Thr Gln Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Val Ser Ala
465 470 475 480
Pro Ser Gly Arg Trp Ser Ser Leu His Arg Gly Phe Ser Ala
485 490
<210> 21
<211> 480
<212> PRT
<213> Glycine max
<400> 21
Met Pro Thr Phe Phe Leu Pro Thr Leu Leu Leu Leu Leu Ile Ala Phe
1 5 10 15
Ala Thr Ser Val Ser Gly Arg Arg Asp Leu Val Gly Asp Phe Leu Arg
20 25 30
Leu Pro Ser Glu Thr Asp Asn Asp Asp Asn Phe Lys Gly Thr Arg Trp
35 40 45
Ala Val Leu Leu Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln
50 55 60
Ala Asp Val Cys His Ala Tyr Gln Ile Leu Arg Lys Gly Gly Leu Lys
65 70 75 80
Glu Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Phe Asn Gly
85 90 95
Glu Asn Pro Arg Pro Gly Val Ile Ile Asn Lys Pro Asp Gly Gly Asp
100 105 110
Val Tyr Lys Gly Val Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val
115 120 125
Asp Asn Phe Phe Ala Ala Leu Leu Gly Asn Lys Ser Ala Leu Thr Gly
130 135 140
Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asp Asp His Ile Phe Val
145 150 155 160
Tyr Tyr Thr Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Ala Gly
165 170 175
Pro Tyr Leu Tyr Ala Asp Asp Leu Ile Glu Val Leu Lys Lys Lys His
180 185 190
Ala Ser Gly Thr Tyr Lys Asn Leu Val Phe Tyr Leu Glu Ala Cys Glu
195 200 205
Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Asp Ile Asn Ile Tyr
210 215 220
Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys
225 230 235 240
Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Thr Thr Cys Leu Gly
245 250 255
Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Arg His Asn Leu
260 265 270
Arg Thr Glu Thr Leu His Gln Gln Tyr Lys Leu Val Lys Glu Arg Thr
275 280 285
Ile Ser Gly Asp Ser Tyr Tyr Gly Ser His Val Met Gln Tyr Gly Asp
290 295 300
Val Gly Leu Ser Arg Asp Val Leu Phe His Tyr Leu Gly Thr Asp Pro
305 310 315 320
Ala Asn Asp Asn Phe Thr Phe Val Asp Glu Asn Ser Leu Trp Ser Pro
325 330 335
Ser Lys Pro Val Asn Gln Arg Asp Ala Asp Leu Ile His Phe Trp Asp
340 345 350
Lys Phe Arg Lys Ala Pro Glu Gly Ser Leu Arg Lys Asn Thr Ala Gln
355 360 365
Lys Gln Val Leu Glu Ala Met Ser His Arg Met His Val Asp Asn Ser
370 375 380
Val Lys Leu Ile Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly Pro Glu
385 390 395 400
Val Leu Asn Ala Val Arg Pro Ala Gly Ser Ala Leu Val Asp Asp Trp
405 410 415
His Cys Leu Lys Thr Met Val Arg Thr Phe Glu Thr His Cys Gly Ser
420 425 430
Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Ile Cys
435 440 445
Asn Val Gly Ile Lys Asn Glu Gln Met Ala Glu Ala Ser Ala Gln Ala
450 455 460
Cys Val Ser Ile Pro Ser Asn Pro Trp Ser Ser Leu Gln Arg Gly Phe
465 470 475 480
<210> 22
<211> 481
<212> PRT
<213> Glycine max
<400> 22
Met Ala Thr Leu Leu Leu Pro Thr Leu Leu Leu Leu Ile Pro Phe Ala
1 5 10 15
Thr Leu Val Ser Ala Arg Pro His Leu Ala Gly Asp Phe Leu Arg Leu
20 25 30
Pro Ser Glu Thr Asp Asn Asp Asp Asn Val Gln Gly Thr Arg Trp Ala
35 40 45
Val Leu Leu Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala
50 55 60
Asp Val Cys His Ala Tyr Gln Ile Leu Arg Lys Gly Gly Leu Lys Glu
65 70 75 80
Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Phe Asn Gly Glu
85 90 95
Asn Pro Arg Pro Gly Val Ile Ile Asn Lys Pro Asp Gly Gly Asp Val
100 105 110
Tyr Glu Gly Val Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val Gly
115 120 125
Asn Phe Phe Ala Ala Leu Leu Gly Asn Lys Ser Ala Leu Thr Gly Gly
130 135 140
Ser Gly Lys Val Val Asp Ser Gly Pro Asp Asp His Ile Phe Val Tyr
145 150 155 160
Tyr Thr Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Ala Gly Pro
165 170 175
Tyr Leu Tyr Ala Asp Asp Leu Ile Glu Val Leu Lys Lys Lys His Ala
180 185 190
Ser Gly Thr Tyr Lys Asn Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser
195 200 205
Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Asp Ile Asn Ile Tyr Ala
210 215 220
Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro
225 230 235 240
Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Ser Thr Cys Leu Gly Asp
245 250 255
Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Arg His Asn Leu Arg
260 265 270
Thr Glu Thr Leu His Gln Gln Tyr Lys Leu Val Lys Glu Arg Thr Ile
275 280 285
Ser Gly Asp Ser Tyr Tyr Gly Ser His Val Met Gln Tyr Gly Asp Val
290 295 300
Arg Leu Ser Ser Asp Val Leu Phe His Tyr Leu Gly Thr Asp Pro Ala
305 310 315 320
Asn Asp Asn Phe Thr Phe Val Asp Glu Asn Ser Leu Trp Ser Pro Ser
325 330 335
Lys Pro Val Asn Gln Arg Asp Ala Asp Leu Ile His Phe Trp Asp Lys
340 345 350
Phe Arg Lys Ala Pro Glu Gly Ser Leu Arg Lys Asn Ala Ala Gln Lys
355 360 365
Gln Val Leu Glu Ala Met Ser His Arg Met His Val Asp Asn Ser Val
370 375 380
Lys Leu Ile Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly Pro Glu Val
385 390 395 400
Leu Asn Ala Val Arg Pro Ala Gly Ser Ala Leu Val Asp Asp Trp His
405 410 415
Cys Leu Lys Thr Met Val Arg Thr Phe Glu Thr His Cys Gly Ser Leu
420 425 430
Ser Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn
435 440 445
Val Gly Ile Lys Asn Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys
450 455 460
Val Ser Ile Pro Ser Asn Pro Trp Ser Ser Leu Gln Arg Gly Phe Ser
465 470 475 480
Ala
<210> 23
<211> 494
<212> PRT
<213> Prunus persica
<400> 23
Met Thr Arg Leu Ala Ser Ala Val Val Leu Leu Phe Leu Val Ser Leu
1 5 10 15
Ser Ser Phe Ala Ala Gly Ser Arg Asp Leu Ile Gly Asp Val Leu Arg
20 25 30
Leu Pro Ser Glu Ala Ser Arg Phe Phe Gly Arg Gly Asp Asp Gly Pro
35 40 45
Asp Glu Gln Asp Asp Gly Thr Val Gly Thr Arg Trp Ala Val Leu Ile
50 55 60
Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys
65 70 75 80
His Ala Tyr Gln Leu Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile
85 90 95
Val Val Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Glu Asn Pro Arg
100 105 110
Pro Gly Val Ile Ile Asn Ser Pro His Gly Asp Asp Val Tyr Lys Gly
115 120 125
Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Thr Val Asn Asn Phe Phe
130 135 140
Ala Ala Ile Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Thr Gly Lys
145 150 155 160
Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp
165 170 175
His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Pro Tyr Ile Tyr
180 185 190
Ala Asn Asp Leu Ile Glu Val Leu Lys Lys Lys His Ala Ala Gly Thr
195 200 205
Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile
210 215 220
Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Phe Ala Thr Thr Ala
225 230 235 240
Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr
245 250 255
Pro Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser
260 265 270
Val Ala Trp Met Glu Asp Ser Asp Ile His Asn Leu Arg Ser Glu Thr
275 280 285
Leu His Gln Gln Tyr Glu Leu Val Lys Thr Arg Thr Ala Asn Asp Asn
290 295 300
Ser Gly Phe Gly Ser His Val Met Gln Tyr Gly Asp Val Gly Leu Ser
305 310 315 320
Lys Glu Asn Leu Phe Val Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn
325 330 335
Tyr Thr Phe Leu Gly Glu Asn Ser Leu Arg Pro Ser Thr Lys Ala Val
340 345 350
Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp His Lys Tyr Arg Lys
355 360 365
Ala Pro Glu Gly Ser Pro Arg Lys Ile Gln Ala Gln Lys Asp Phe Val
370 375 380
Glu Ala Met Ser His Arg Met His Met Asp Gln Thr Met Lys Leu Ile
385 390 395 400
Gly Lys Leu Leu Phe Gly Ile Lys Lys Gly Pro Glu Val Leu Asn Thr
405 410 415
Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys
420 425 430
Thr Met Val Arg Ser Phe Glu Thr Tyr Cys Gly Ser Leu Ser Gln Tyr
435 440 445
Gly Met Lys His Met Arg Ser Leu Ala Asn Ile Cys Asn Ala Gly Met
450 455 460
Thr Lys Glu Gln Met Thr Glu Ala Ser Ala Gln Ala Cys Thr Ser Val
465 470 475 480
Pro Ser Ser Arg Trp Ser Ser Leu His Arg Gly Phe Ser Ala
485 490
<210> 24
<211> 487
<212> PRT
<213> Arachis diogoi
<400> 24
Met Glu Ser Leu Leu Arg Ile Thr Leu Leu Phe Phe Ala Phe Thr Thr
1 5 10 15
Phe Val Ala Ser Ala Ser Gly Arg Arg Asp Ile Val Gly Gly Thr Leu
20 25 30
Arg Leu Pro Ser Glu Ala Ile Ser Arg Phe Phe His Glu Pro Glu Asn
35 40 45
Glu Gly Thr Lys Trp Ala Val Leu Leu Ala Gly Ser Asn Gly Tyr Trp
50 55 60
Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln Leu Leu Arg
65 70 75 80
Ser Gly Gly Val Lys Glu Glu Asn Ile Ile Val Phe Met Phe Asp Asp
85 90 95
Ile Ala Tyr Ser Glu Glu Asn Pro Arg Pro Gly Val Ile Ile Asn Lys
100 105 110
Pro Asp Gly Gly Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Thr Gly
115 120 125
Lys Asp Val Asn Val Asn Asn Phe Phe Ala Ala Leu Leu Gly Asn Lys
130 135 140
Ser Ala Leu Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn
145 150 155 160
Asp His Ile Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Ile Leu
165 170 175
Gly Met Pro Val Gly Pro Tyr Leu Tyr Ala Asn Asp Leu Asn Glu Val
180 185 190
Leu Lys Lys Lys His Ala Ser Gly Gly Tyr Lys Ser Leu Val Phe Tyr
195 200 205
Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu
210 215 220
Asp Ile Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Val Glu Ser Ser
225 230 235 240
Trp Gly Thr Tyr Cys Pro Gly Glu Asp Pro Ser Pro Pro Pro Glu Tyr
245 250 255
Ser Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ser Trp Met Glu Asp Ser
260 265 270
Asp Thr His Asn Leu Arg Thr Glu Thr Leu His Gln Gln Tyr Lys Leu
275 280 285
Val Lys Asp Arg Thr Leu Asn Gly Asn Ala Tyr Tyr Gly Ser His Ala
290 295 300
Met Gln Tyr Gly Asp Val Gly Ile Ser Glu Asn Leu Leu Phe Gln Tyr
305 310 315 320
Leu Gly Thr Asn Pro Ala Asn Asp Asn Tyr Thr Phe Val Asp Glu Asn
325 330 335
Ser Leu Arg Thr Pro Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu
340 345 350
Ile His Phe Trp Glu Lys Phe Arg Lys Ala Pro Glu Gly Ser Ser Ser
355 360 365
Lys Ile Thr Ala Gln Lys Gln Val Val Glu Val Met Ser His Arg Met
370 375 380
His Ile Asp Asn Ser Val Lys Leu Ile Gly Asn Leu Leu Phe Gly Thr
385 390 395 400
Glu Lys Gly Pro Glu Leu Leu Ser Ala Val Arg Pro Ala Gly Lys Pro
405 410 415
Leu Val Asp Asp Trp Asp Cys Leu Lys Asn Met Val Arg Thr Phe Glu
420 425 430
Thr His Cys Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Thr
435 440 445
Phe Ala Asn Ile Cys Asn Ala Gly Ile His Lys Asp Gln Met Asp Glu
450 455 460
Ala Thr Ala Gln Ala Cys Val Ser Ile Pro Ser Asn Pro Trp Ser Ser
465 470 475 480
Leu Glu Arg Gly Phe Ser Ala
485
<210> 25
<211> 493
<212> PRT
<213> Populus trichocarpa
<400> 25
Met Thr Arg Leu Ile Ala Gly Val Ile Phe Leu Leu Ile Ser Phe Cys
1 5 10 15
Gly Ile Ala Val Gly Val Arg Asp Ile Val Gly Asp Val Leu Arg Leu
20 25 30
Pro Ser Glu Ala Ser Arg Phe Phe Arg Pro Gly Lys Phe Asn Asp Asp
35 40 45
Asn Ser Asp Asp Asp Ser Ser Gly Thr Arg Trp Ala Ile Leu Leu Ala
50 55 60
Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His
65 70 75 80
Ala Tyr Gln Leu Leu Arg Gln Gly Gly Leu Lys Glu Glu Asn Ile Ile
85 90 95
Val Phe Met Tyr Asp Asp Ile Ala Asp Asn Pro Glu Asn Pro Arg Pro
100 105 110
Gly Val Ile Ile Asn Asn Pro Gln Gly Glu Asp Val Tyr Lys Gly Val
115 120 125
Pro Lys Asp Tyr Thr Gly Pro Asp Val Thr Val Gly Asn Phe Phe Ala
130 135 140
Ala Ile Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser Gly Lys Val
145 150 155 160
Ile Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His
165 170 175
Gly Gly Pro Gly Val Leu Gly Met Pro Thr Asn Pro Tyr Leu Tyr Ala
180 185 190
Asp Asp Leu Ile Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr
195 200 205
Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe
210 215 220
Glu Gly Leu Leu Pro Gln Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser
225 230 235 240
Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro
245 250 255
Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val
260 265 270
Ala Trp Met Glu Asp Ser Asp Ile His Asn Leu Arg Thr Glu Thr Leu
275 280 285
His Gln Gln Tyr Glu Leu Val Lys Arg Arg Thr Ser Tyr Asp Asn Ser
290 295 300
Pro Tyr Gly Ser His Val Met Gln Tyr Gly Asp Val Gly Leu Ser Lys
305 310 315 320
Asp Asp Leu Phe Gln Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Tyr
325 330 335
Thr Phe Val Glu Glu Asn Ser Leu Arg Pro His Ser Lys Val Val Asn
340 345 350
Gln Arg Asp Ala Asp Leu Val His Phe Trp Thr Lys Tyr Arg Lys Ala
355 360 365
Pro Glu Gly Ser Ser Arg Lys Val Glu Ala Gln Lys Gln Phe Val Glu
370 375 380
Ala Met Ser His Arg Met His Ile Asp His Ser Ile Lys Leu Ile Gly
385 390 395 400
Lys Leu Leu Phe Gly Ile Glu Lys Ala Ser Glu Ala Leu Asn Thr Val
405 410 415
Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Val Cys Leu Lys Thr
420 425 430
Leu Val Arg Thr Phe Glu Thr His Cys Gly Ser Ile Ser Gln Tyr Gly
435 440 445
Met Lys His Met Arg Ser Leu Ala Asn Leu Cys Asn Ala Gly Ile Val
450 455 460
Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Val Ser Phe Pro
465 470 475 480
Ser Gly Ser Trp Ser Ser Leu His Lys Gly Phe Ser Ala
485 490
<210> 26
<211> 492
<212> PRT
<213> Cicer arietinum
<400> 26
Met Asp Arg Ser Lys Ile Ser Thr Leu Leu Phe Leu Ile Val Ala Leu
1 5 10 15
Thr Phe Leu Ala Ala Val Ser Ala Val Arg Asp Leu Pro Gly Asp Tyr
20 25 30
Leu Arg Leu Pro Ser Asp Ala Ser Arg Phe Phe His Glu Pro Glu Asn
35 40 45
Asp Asp Asn Val Gln Gly Thr Arg Trp Ala Ile Leu Leu Ala Gly Ser
50 55 60
Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr
65 70 75 80
Gln Ile Leu Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile Ile Val Phe
85 90 95
Met Tyr Asp Asp Ile Ala Phe Asp Ile Glu Asn Pro Arg Pro Gly Val
100 105 110
Ile Ile Asn Lys Pro Asp Gly Gly Asp Val Tyr Ala Gly Val Pro Lys
115 120 125
Asp Tyr Thr Gly Asp Asp Val Asn Val Asp Asn Phe Tyr Ala Ala Leu
130 135 140
Leu Gly Asn Lys Ser Ala Leu Thr Gly Gly Ser Gly Lys Val Val Asp
145 150 155 160
Ser Gly Pro Asn Asp His Ile Phe Val Tyr Tyr Thr Asp His Gly Gly
165 170 175
Pro Gly Val Leu Gly Met Pro Val Gly Pro Tyr Leu Tyr Ala Ser Asp
180 185 190
Leu Asn Glu Val Leu Lys Lys Lys His Ala Ser Gly Ser Tyr Lys Ser
195 200 205
Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly
210 215 220
Leu Leu Pro Glu Asn Ile Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala
225 230 235 240
Asp Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Pro Pro
245 250 255
Pro Pro Glu Tyr Ser Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp
260 265 270
Met Glu Asp Ser Asp Ile His Asn Leu Arg Thr Glu Ser Leu His Gln
275 280 285
Gln Tyr Lys Val Val Lys Asp Arg Thr Ile Asn Gly Val Tyr Tyr Gly
290 295 300
Ser His Val Met Glu Tyr Gly Asp Val Gly Leu Ser Asn Asn His Leu
305 310 315 320
Phe Ile Tyr Leu Gly Thr Asn Pro Ala Asn Asp Asn Ile Ser Phe Val
325 330 335
Asp Glu Ser Ser Leu Thr Leu Arg Ser Pro Ser Ala Ala Val Asn Gln
340 345 350
Arg Asp Ala Asp Leu Ile His Phe Trp Asp Lys Phe Arg Lys Ala Pro
355 360 365
Glu Gly Ser Ala Arg Lys Asn Glu Ala Glu Lys Gln Val Leu Glu Ala
370 375 380
Met Ser His Arg Lys His Val Asp Asn Ser Val Glu Leu Ile Gly Lys
385 390 395 400
Leu Leu Phe Gly Ile Glu Lys Gly Pro Glu Leu Phe Asn Thr Val Arg
405 410 415
Pro Ala Gly Leu Pro Leu Val Asp Asn Trp Asp Cys Leu Lys Thr Met
420 425 430
Val Arg Thr Phe Glu Thr His Cys Gly Ser Leu Ser Gln Tyr Gly Met
435 440 445
Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile Gln Asn
450 455 460
Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Ser Asn Ile Pro Ala
465 470 475 480
Asn Pro Trp Ser Ser Leu His Arg Gly Phe Ser Ala
485 490
<210> 27
<211> 493
<212> PRT
<213> Vitis vinifera
<400> 27
Met Thr Ile Phe Pro Ala Ala Val Ala Ala Phe Leu Ala Leu Ser Thr
1 5 10 15
Leu Val Ala Gly Gly Arg His Phe Ala Gly Asp Asn Gly Leu Leu Leu
20 25 30
Pro Ser Glu Ala Ser Arg Phe Phe Arg Pro Gly Gly Ala Ala Asp Asp
35 40 45
Asp Thr Gly Ala Glu Ser Ala Gly Thr Arg Trp Ala Val Leu Ile Ala
50 55 60
Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys His
65 70 75 80
Ala Tyr Gln Leu Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile
85 90 95
Val Phe Met Tyr Asp Asp Ile Ser Phe Asn Glu Glu Asn Pro Arg Pro
100 105 110
Gly Ile Ile Ile Asn Ser Pro His Gly Glu Asp Val Tyr Glu Gly Val
115 120 125
Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val Asp Asn Phe Phe Ala
130 135 140
Val Ile Leu Gly Asn Lys Thr Ala Leu Ser Gly Gly Ser Gly Lys Val
145 150 155 160
Leu Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His
165 170 175
Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Pro Tyr Leu Tyr Ala
180 185 190
Asn Asp Leu Ile Glu Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr
195 200 205
Asn Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe
210 215 220
Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ala
225 230 235 240
Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Asp Pro
245 250 255
Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val
260 265 270
Ala Trp Met Glu Asp Ser Asp Val His Asn Leu Arg Thr Glu Thr Leu
275 280 285
Arg Gln Gln Tyr Glu Leu Val Lys Lys Arg Thr Ala Asn Asp Asn Ser
290 295 300
Val Tyr Gly Ser His Val Met Gln Tyr Gly Asp Leu Gly Leu Asn Lys
305 310 315 320
Glu Asp Leu Val Leu Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Tyr
325 330 335
Thr Phe Val Asp Asn Asn Ser Leu Arg Leu Pro Ser Lys Ala Val Asn
340 345 350
Gln Arg Asp Ala Asp Leu Val His Phe Trp Asp Lys Phe Arg Lys Ala
355 360 365
Pro Glu Gly Ser Pro Arg Lys Ala Glu Ala Gln Lys Gln Phe Leu Glu
370 375 380
Ala Met Ser His Arg Thr His Ile Asp His Ala Ile Lys Leu Val Gly
385 390 395 400
Arg Leu Leu Phe Gly Met Lys Lys Gly Ser Glu Val Leu Lys Thr Val
405 410 415
Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp His Cys Leu Lys Thr
420 425 430
Leu Val Arg Thr Phe Glu Ala His Cys Gly Ser Leu Ser Gln Tyr Gly
435 440 445
Met Lys His Met Arg Ser Ile Ala Asn Ile Cys Asn Ala Gly Ile Glu
450 455 460
Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Val Thr Ile Pro
465 470 475 480
Pro Gly Pro Trp Ser Ser Leu Asp Lys Gly Phe Ser Ala
485 490
<210> 28
<211> 484
<212> PRT
<213> Phaseolus vulgaris
<400> 28
Met Ala Thr Thr Thr Ala Thr Thr Ser Leu Leu Ala Leu Leu Leu Leu
1 5 10 15
Phe Leu Val Ala Leu Val Ser Ala Gly Arg Asp Leu Val Gly Asp Phe
20 25 30
Leu Arg Leu Pro Ser Asp Ser Gly Asn Gly Asp Asn Val His Gly Thr
35 40 45
Arg Trp Ala Ile Leu Phe Ala Gly Ser Ser Gly Tyr Trp Asn Tyr Arg
50 55 60
His Gln Ala Asp Ile Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly
65 70 75 80
Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Phe
85 90 95
Asn Ser Glu Asn Pro Arg Arg Gly Val Ile Ile Asn Ser Pro Asn Gly
100 105 110
Asp Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Thr Gly Glu Asp Val
115 120 125
Thr Ala His Asn Phe Tyr Ala Ala Leu Leu Gly Asp Lys Ser Lys Leu
130 135 140
Thr Gly Gly Ser Gly Lys Val Val Asn Ser Gly Pro Asn Asp His Ile
145 150 155 160
Phe Ile Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Ser Pro
165 170 175
Ala Gly Pro Tyr Ile Tyr Ala Ser Asp Leu Asn Glu Val Leu Lys Lys
180 185 190
Lys His Ala Ser Gly Thr Tyr Lys Asn Leu Val Phe Tyr Leu Glu Ala
195 200 205
Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Asp Ile Asn
210 215 220
Val Tyr Ala Thr Thr Ala Ser Asn Ala Asp Glu Ser Ser Trp Gly Thr
225 230 235 240
Tyr Cys Pro Gly Glu Asp Pro Ser Pro Pro Pro Glu Tyr Ser Thr Cys
245 250 255
Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Arg His
260 265 270
Asn Leu Arg Thr Glu Thr Leu His Gln Gln Tyr Lys Leu Val Lys Glu
275 280 285
Arg Thr Ile Ser Gly Gly Leu Tyr Tyr Gly Ser His Val Met Gln Tyr
290 295 300
Gly Asp Val Gly Leu Ser Lys Asp Ile Leu Phe His Tyr Leu Gly Thr
305 310 315 320
Asp Pro Ala Asn Glu Asn Leu Thr Phe Val Asp Glu Asn Ser Leu Trp
325 330 335
Ser Ser Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val His Phe
340 345 350
Trp Asp Lys Phe Arg Lys Ala Pro Glu Gly Ser Pro Lys Lys Asn Glu
355 360 365
Ala Arg Lys Gln Val Leu Glu Val Met Ser His Arg Met His Ile Asp
370 375 380
Asp Ser Val Glu Leu Val Gly Lys Leu Leu Phe Gly Ile Glu Lys Ala
385 390 395 400
Pro Glu Leu Leu Asn Ala Val Arg Pro Ala Gly Ser Ala Leu Val Asp
405 410 415
Asp Trp Asp Cys Leu Lys Thr Met Val Arg Thr Phe Glu Thr His Cys
420 425 430
Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn
435 440 445
Met Cys Asn Val Gly Ile Lys Lys Glu Gln Met Arg Glu Ala Ser Ala
450 455 460
Gln Ala Cys Val Thr Ile Pro Ala Asn Pro Trp Ser Ser Leu Gln Arg
465 470 475 480
Gly Phe Ser Ala
<210> 29
<211> 484
<212> PRT
<213> Phaseolus vulgaris
<400> 29
Met Ala Thr Thr Thr Ala Thr Thr Ser Leu Leu Ala Leu Leu Leu Leu
1 5 10 15
Phe Leu Val Ala Leu Val Ser Ala Gly Arg Asp Leu Val Gly Asp Phe
20 25 30
Leu Arg Leu Pro Ser Asp Ser Gly Asn Gly Asp Asn Val His Gly Thr
35 40 45
Arg Trp Ala Ile Leu Phe Ala Gly Ser Ser Gly Tyr Trp Asn Tyr Arg
50 55 60
His Gln Ala Asp Ile Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly
65 70 75 80
Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Phe
85 90 95
Asn Ser Glu Asn Pro Arg Arg Gly Val Ile Ile Asn Ser Pro Asn Gly
100 105 110
Asp Glu Val Tyr Lys Gly Val Pro Lys Asp Tyr Thr Gly Glu Asp Val
115 120 125
Thr Ala His Asn Phe Tyr Ala Ala Leu Leu Gly Asp Lys Ser Lys Leu
130 135 140
Thr Gly Gly Ser Gly Lys Val Val Asn Ser Gly Pro Asn Asp His Ile
145 150 155 160
Phe Ile Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Ser Pro
165 170 175
Ala Gly Pro Tyr Ile Tyr Ala Ser Asp Leu Asn Glu Val Leu Lys Lys
180 185 190
Lys His Ala Ser Gly Thr Tyr Lys Asn Leu Val Phe Tyr Leu Glu Ala
195 200 205
Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Asp Ile Asn
210 215 220
Val Tyr Ala Thr Thr Ala Ser Asn Ala Asp Glu Ser Ser Trp Gly Thr
225 230 235 240
Tyr Cys Pro Gly Glu Asp Pro Ser Pro Pro Pro Glu Tyr Ser Thr Cys
245 250 255
Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Arg His
260 265 270
Asn Leu Arg Thr Glu Thr Leu His Gln Gln Tyr Lys Leu Val Lys Glu
275 280 285
Arg Thr Ile Ser Gly Gly Leu Tyr Tyr Gly Ser His Val Met Gln Tyr
290 295 300
Gly Asp Val Gly Leu Ser Lys Asp Ile Leu Phe His Tyr Leu Gly Thr
305 310 315 320
Asp Pro Ala Asn Glu Asn Leu Thr Phe Val Asp Glu Asn Ser Leu Trp
325 330 335
Ser Ser Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val His Phe
340 345 350
Trp Asp Lys Phe Arg Lys Ala Pro Glu Gly Ser Pro Lys Lys Asn Glu
355 360 365
Ala Arg Lys Gln Val Leu Glu Val Met Ser His Arg Met His Ile Asp
370 375 380
Asp Ser Val Glu Leu Val Gly Lys Leu Leu Phe Gly Ile Glu Lys Ala
385 390 395 400
Pro Glu Leu Leu Asn Ala Val Arg Pro Ala Gly Ser Ala Leu Val Asp
405 410 415
Asp Trp Asp Cys Leu Lys Thr Met Val Arg Thr Phe Glu Thr His Cys
420 425 430
Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn
435 440 445
Met Cys Asn Val Gly Ile Lys Lys Glu Gln Met Arg Glu Ala Ser Ala
450 455 460
Gln Ala Cys Val Thr Ile Pro Ala Asn Pro Trp Ser Ser Leu Gln Arg
465 470 475 480
Gly Phe Ser Ala
<210> 30
<211> 493
<212> PRT
<213> Vitis vinifera
<400> 30
Met Thr Ile Phe Pro Ala Ala Val Ala Ala Leu Leu Ala Leu Ser Thr
1 5 10 15
Leu Val Ala Gly Gly Arg His Phe Ala Gly Asp Asn Gly Leu Leu Leu
20 25 30
Pro Ser Glu Ala Ser Arg Phe Phe Arg Pro Gly Gly Ala Ala Asp Asp
35 40 45
Asp Thr Gly Gly Glu Ser Ala Gly Thr Arg Trp Ala Val Leu Ile Ala
50 55 60
Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys His
65 70 75 80
Ala Tyr Gln Leu Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile
85 90 95
Val Phe Met Tyr Asp Asp Ile Ser Phe Asn Glu Glu Asn Pro Arg Pro
100 105 110
Gly Ile Ile Ile Asn Ser Pro His Gly Glu Asp Val Tyr Glu Gly Val
115 120 125
Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val Asp Asn Phe Phe Ala
130 135 140
Val Ile Leu Gly Asn Lys Thr Ala Leu Ser Gly Gly Ser Gly Lys Val
145 150 155 160
Leu Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His
165 170 175
Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Pro Tyr Leu Tyr Ala
180 185 190
Asn Asp Leu Ile Glu Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr
195 200 205
Asn Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe
210 215 220
Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ala
225 230 235 240
Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Asp Pro
245 250 255
Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val
260 265 270
Ala Trp Met Glu Asp Ser Asp Val His Asn Leu Arg Thr Glu Thr Leu
275 280 285
Arg Gln Gln Tyr Glu Leu Val Lys Lys Arg Thr Ala Asn Asp Asn Ser
290 295 300
Val Tyr Gly Ser His Val Met Gln Tyr Gly Asp Leu Gly Leu Asn Lys
305 310 315 320
Glu Asp Leu Val Leu Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Tyr
325 330 335
Thr Phe Val Asp Asn Asn Ser Leu Arg Leu Pro Ser Lys Ala Val Asn
340 345 350
Gln Arg Asp Ala Asp Leu Val His Phe Trp Asp Lys Phe Arg Lys Ala
355 360 365
Pro Glu Gly Ser Pro Arg Lys Ala Glu Ala Gln Lys Gln Phe Leu Glu
370 375 380
Ala Met Ser His Arg Thr His Ile Asp His Ala Ile Lys Leu Val Gly
385 390 395 400
Arg Leu Leu Phe Gly Met Lys Lys Gly Ser Glu Val Leu Lys Thr Val
405 410 415
Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp His Cys Leu Lys Thr
420 425 430
Leu Val Arg Thr Phe Glu Ala His Cys Gly Ser Leu Ser Gln Tyr Gly
435 440 445
Met Lys His Met Arg Ser Ile Ala Asn Ile Cys Asn Ala Gly Ile Glu
450 455 460
Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Val Thr Ile Pro
465 470 475 480
Pro Gly Pro Trp Ser Ser Leu Asp Lys Gly Phe Ser Ala
485 490
<210> 31
<211> 493
<212> PRT
<213> Cucumis sativus
<400> 31
Met Ala Arg Ile Pro Thr Gly Val Leu Leu Ser Leu Leu Phe Leu Ala
1 5 10 15
Val Ile Gly Leu Pro Ala Gly Ala Arg Asp Leu Pro Gly Asp Phe Leu
20 25 30
Arg Leu Pro Ser Glu Ala Leu Lys Phe Phe Arg Gly Gly Ala Ser Asp
35 40 45
Ala Ser Asp Glu Asp Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala
50 55 60
Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys His
65 70 75 80
Ala Tyr Gln Leu Leu Arg Lys Asn Gly Leu Lys Asp Glu Asn Ile Ile
85 90 95
Val Phe Met Tyr Asp Asp Ile Ala Phe Asn Pro Glu Asn Pro Arg Pro
100 105 110
Gly Val Ile Ile Asn His Pro Lys Gly Ser Asp Val Tyr His Gly Val
115 120 125
Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val Asn Asn Phe Phe Ala
130 135 140
Ala Ile Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser Gly Lys Val
145 150 155 160
Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His
165 170 175
Gly Gly Pro Gly Val Leu Gly Met Pro Thr Tyr Pro Tyr Met Tyr Ala
180 185 190
Asp Asp Leu Asn Lys Val Leu Lys Lys Lys His Ala Ala Gly Ser Tyr
195 200 205
Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe
210 215 220
Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Thr Thr Thr Ala Ser
225 230 235 240
Asn Ala Tyr Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Asp Tyr Pro
245 250 255
Ser Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly Asp Leu Tyr Ser Val
260 265 270
Ala Trp Leu Glu Asp Ser Asp Asn His Asn Leu Lys Thr Glu Ser Leu
275 280 285
Arg Gln Gln Tyr Glu Leu Val Lys Lys Arg Thr Ile Ser Gly Gln Tyr
290 295 300
Ala Tyr Gly Ser His Val Met Gln Tyr Gly Asp Leu Met Leu Asn Lys
305 310 315 320
Asn Ala Leu Phe Ser Tyr Leu Gly Thr Asp Pro Ala Asn Glu Asn Asn
325 330 335
Thr Phe Val Glu Glu Asn Ser Leu Arg Pro Ala Thr Lys Phe Thr Asn
340 345 350
Gln Arg Asp Ala Asp Leu Val His Phe Trp Glu Lys Phe Arg Lys Ala
355 360 365
Pro Glu Gly Ser Leu Thr Lys Val Glu Ala Gln Lys Lys Phe Val Glu
370 375 380
Ala Met Ser His Arg Ala His Ile Asp Asn Ser Val Lys Leu Val Gly
385 390 395 400
Lys Leu Leu Phe Gly Ile Lys Glu Gly Pro Glu Val Leu Glu Ala Ile
405 410 415
Arg Pro Ala Gly Arg Pro Leu Val Asp Asp Trp Asn Cys Leu Arg Asn
420 425 430
Met Val Arg Ser Phe Glu Ala Arg Cys Gly Ser Leu Ser Gln Tyr Gly
435 440 445
Met Lys His Met Arg Ser Phe Ala Asn Leu Cys Asn Ala Gly Ile Ser
450 455 460
Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Met Ser Val Pro
465 470 475 480
Pro Gly Pro Trp Ser Ser Leu Leu Lys Gly Phe Thr Ala
485 490
<210> 32
<211> 493
<212> PRT
<213> Cucumis sativus
<400> 32
Met Ala Arg Ile Pro Thr Gly Val Leu Leu Ser Leu Leu Phe Leu Ala
1 5 10 15
Val Ile Gly Leu Pro Ala Gly Ala Arg Asp Leu Pro Gly Asp Phe Leu
20 25 30
Arg Leu Pro Ser Glu Ala Leu Lys Phe Phe Arg Gly Gly Ala Ser Asp
35 40 45
Ala Ser Asp Glu Asp Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala
50 55 60
Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys His
65 70 75 80
Ala Tyr Gln Leu Leu Arg Lys Asn Gly Leu Lys Asp Glu Asn Ile Ile
85 90 95
Val Phe Met Tyr Asp Asp Ile Ala Phe Asn Pro Glu Asn Pro Arg Pro
100 105 110
Gly Val Ile Ile Asn His Pro Lys Gly Ser Asp Val Tyr His Gly Val
115 120 125
Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val Asn Asn Phe Phe Ala
130 135 140
Ala Ile Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser Gly Lys Val
145 150 155 160
Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His
165 170 175
Gly Gly Pro Gly Val Leu Gly Met Pro Thr Tyr Pro Tyr Met Tyr Ala
180 185 190
Asp Asp Leu Asn Lys Val Leu Lys Lys Lys His Ala Ala Gly Ser Tyr
195 200 205
Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe
210 215 220
Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Thr Thr Thr Ala Ser
225 230 235 240
Asn Ala Tyr Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Asp Tyr Pro
245 250 255
Ser Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly Asp Leu Tyr Ser Val
260 265 270
Ala Trp Leu Glu Asp Ser Asp Asn His Asn Leu Lys Thr Glu Ser Leu
275 280 285
Arg Gln Gln Tyr Glu Leu Val Lys Lys Arg Thr Leu Ser Gly Gln Tyr
290 295 300
Ala Tyr Gly Ser His Val Met Gln Tyr Gly Asp Leu Met Leu Asn Lys
305 310 315 320
Asn Ala Leu Phe Ser Tyr Leu Gly Thr Asp Pro Ala Asn Glu Asn Asn
325 330 335
Thr Phe Val Glu Glu Asn Ser Leu Arg Pro Ala Thr Lys Phe Thr Asn
340 345 350
Gln Arg Asp Ala Asp Leu Val His Phe Trp Glu Lys Phe Arg Lys Ala
355 360 365
Pro Glu Gly Ser Leu Thr Lys Val Glu Ala Gln Lys Lys Phe Val Glu
370 375 380
Ala Met Ser His Arg Ala His Ile Asp Asn Ser Val Lys Leu Val Gly
385 390 395 400
Lys Leu Leu Phe Gly Ile Lys Glu Gly Pro Glu Val Leu Glu Ala Ile
405 410 415
Arg Pro Ala Gly Arg Pro Leu Val Asp Asp Trp Asn Cys Leu Arg Asn
420 425 430
Met Val Arg Ser Phe Glu Ala Arg Cys Gly Ser Leu Ser Gln Tyr Gly
435 440 445
Met Lys His Met Arg Ser Phe Ala Asn Leu Cys Asn Ala Gly Ile Ser
450 455 460
Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Met Ser Val Pro
465 470 475 480
Pro Gly Pro Trp Ser Ser Leu Leu Lys Gly Phe Thr Ala
485 490
<210> 33
<211> 493
<212> PRT
<213> Vicia sativa
<400> 33
Met Gly Ser Ser Gln Leu Ser Thr Leu Leu Phe Phe Thr Ile Val Val
1 5 10 15
Thr Phe Leu Thr Val Val Ser Ser Gly Arg Asp Leu Pro Gly Asp Tyr
20 25 30
Leu Arg Leu Pro Ser Glu Thr Ser Arg Phe Phe Arg Glu Pro Lys Asn
35 40 45
Asp Asp Asp Phe Glu Gly Thr Arg Trp Ala Ile Leu Leu Ala Gly Ser
50 55 60
Asn Gly Tyr Trp Asn Tyr Arg His Gln Ser Asp Val Cys His Ala Tyr
65 70 75 80
Gln Leu Leu Arg Lys Gly Gly Ser Lys Glu Glu Asn Ile Ile Val Phe
85 90 95
Met Tyr Asp Asp Ile Ala Ser Asn Glu Glu Asn Pro Arg Pro Gly Val
100 105 110
Ile Ile Asn Lys Pro Asp Gly Asp Asp Val Tyr Ala Gly Val Pro Lys
115 120 125
Asp Tyr Thr Gly Ala Glu Val His Ala Asp Asn Phe Tyr Ala Ala Leu
130 135 140
Leu Gly Asn Lys Ser Ala Leu Thr Gly Gly Ser Gly Lys Val Val Asp
145 150 155 160
Ser Gly Pro Asn Asp His Ile Phe Val Tyr Tyr Thr Asp His Gly Gly
165 170 175
Pro Gly Val Leu Gly Met Pro Val Gly Pro Tyr Leu Tyr Ala Ser Asp
180 185 190
Leu Asn Glu Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser
195 200 205
Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly
210 215 220
Leu Leu Pro Asp Asp Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala
225 230 235 240
Glu Glu Ser Ser Trp Gly Tyr Tyr Cys Pro Gly Asp Lys Pro Pro Pro
245 250 255
Pro Pro Glu Tyr Ser Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ala Trp
260 265 270
Met Glu Asp Ser Glu Val His Asn Leu Gln Thr Glu Ser Leu Gln Gln
275 280 285
Gln Tyr Lys Leu Val Lys Asn Arg Thr Ile Ser Glu Pro Tyr Gly Ser
290 295 300
His Val Met Glu Tyr Gly Asp Ile Gly Leu Ser Lys Asn Asp Leu Tyr
305 310 315 320
Gln Tyr Leu Gly Thr Asn Pro Ala Asn Asp Asn Asn Ser Phe Val Asp
325 330 335
Glu Thr Glu Asn Ser Leu Lys Leu Arg Thr Pro Ser Ala Ala Val Asn
340 345 350
Gln Arg Asp Ala Asp Leu Ile His Phe Trp Glu Lys Phe Arg Lys Ala
355 360 365
Pro Glu Gly Ser Ser Gln Lys Asn Glu Ala Glu Lys Gln Val Leu Glu
370 375 380
Ala Met Ser His Arg Lys His Ile Asp Asn Ser Val Lys Leu Ile Gly
385 390 395 400
Gln Leu Leu Phe Gly Ile Glu Lys Gly Thr Glu Leu Leu Asp Val Val
405 410 415
Arg Pro Ala Gly Ser Pro Leu Val Asp Asn Trp Asp Cys Leu Lys Thr
420 425 430
Met Val Lys Thr Phe Glu Thr His Cys Gly Ser Leu Ser Gln Tyr Gly
435 440 445
Met Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile Pro
450 455 460
Asn Glu Pro Met Ala Glu Ala Ser Ala Gln Ala Cys Ala Ser Ile Pro
465 470 475 480
Ala Asn Pro Trp Ser Ser Leu Gln Gly Gly Phe Ser Ala
485 490
<210> 34
<211> 493
<212> PRT
<213> Medicago truncatula
<400> 34
Met Asp Phe Ser Gln Phe Ser Thr Ile Leu Phe Leu Thr Val Ile Leu
1 5 10 15
Thr Ile Phe Ala Ala Val Ser Gly Ser Arg Asp Leu Pro Gly Asp Tyr
20 25 30
Ile Arg Leu Pro Ser Gln Ser Gln Ala Ser Arg Phe Phe His Glu Pro
35 40 45
Glu Asn Asp Asp Asn Asp Gln Gly Thr Arg Trp Ala Ile Leu Leu Ala
50 55 60
Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His
65 70 75 80
Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile Ile
85 90 95
Val Phe Met Tyr Asp Asp Ile Ala Ser Asn Val Glu Asn Pro Arg Pro
100 105 110
Gly Val Ile Ile Asn Lys Pro Asp Gly Gly Asp Val Tyr Glu Gly Val
115 120 125
Pro Lys Asp Tyr Thr Gly Ala Glu Val His Ala Asp Asn Phe Tyr Ala
130 135 140
Ala Leu Leu Gly Asn Lys Ser Ala Leu Thr Gly Gly Ser Gly Lys Val
145 150 155 160
Val Asp Ser Gly Pro Asn Asp His Ile Phe Val Tyr Tyr Thr Asp His
165 170 175
Gly Gly Pro Gly Val Leu Gly Met Pro Val Gly Pro Tyr Leu Tyr Ala
180 185 190
Ser Asp Leu Asn Glu Val Leu Lys Lys Lys His Ala Ser Gly Ser Tyr
195 200 205
Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe
210 215 220
Glu Gly Leu Leu Pro Glu Asp Ile Asn Ile Tyr Ala Thr Thr Ala Ser
225 230 235 240
Asn Ala Val Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro
245 250 255
Pro Pro Pro Pro Glu Tyr Ser Thr Cys Leu Gly Asp Leu Tyr Ser Ile
260 265 270
Ala Trp Met Glu Asp Ser Asp Ile His Asn Leu Arg Thr Glu Ser Leu
275 280 285
His Gln Gln Tyr Lys Leu Val Lys Asp Arg Thr Ile Asn Gly Tyr Tyr
290 295 300
Gly Ser His Val Met Glu Tyr Gly Asp Val Gly Leu Ser Asn Asn His
305 310 315 320
Leu Phe Leu Tyr Leu Gly Thr Asn Pro Ala Asn Asp Asn Ile Ser Phe
325 330 335
Val Asp Glu Ser Ser Leu Lys Leu Arg Ser Pro Ser Thr Ala Val Asn
340 345 350
Gln Arg Asp Ala Asp Leu Ile His Phe Trp Asp Lys Phe Arg Lys Ala
355 360 365
Pro Glu Gly Ser Leu Arg Lys Asn Glu Ala Gln Lys Glu Val Leu Glu
370 375 380
Ala Met Ser His Arg Met His Val Asp Asn Ser Ala Lys Leu Ile Gly
385 390 395 400
Lys Leu Leu Phe Gly Ile Glu Lys Gly Thr Glu Leu Leu Gly Asn Val
405 410 415
Arg Pro Ala Gly Ser Pro Leu Val Asp Asn Trp Asp Cys Leu Lys Thr
420 425 430
Met Val Lys Thr Phe Glu Thr His Cys Gly Ser Leu Ser Gln Tyr Gly
435 440 445
Met Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile Gln
450 455 460
Thr Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Ala Ser Ile Pro
465 470 475 480
Ala Asn Pro Trp Ser Ser Leu Gln Arg Gly Phe Ser Ala
485 490
<210> 35
<211> 494
<212> PRT
<213> Fragaria vesca subsp. vesca
<400> 35
Met Thr Arg Leu Ala Ala Val Val Ala Val Val Leu Phe Phe Leu Val
1 5 10 15
Ser Leu Phe Ser Ser Ser Thr Ser Ala Arg Asp Leu Pro Gly Asp Val
20 25 30
Leu Arg Leu Pro Ser Glu Thr Ser Arg Phe Phe Arg Ala Gly Asp Asp
35 40 45
Gln Gln Asp Asp Gly Thr Val Gly Thr Arg Trp Ala Val Leu Ile Ala
50 55 60
Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys His
65 70 75 80
Ala Tyr Gln Leu Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Val
85 90 95
Val Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Glu Asn Pro Arg Gln
100 105 110
Gly Val Ile Ile Asn Ser Pro His Gly Asp Asp Val Tyr Lys Gly Val
115 120 125
Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val Gly Asn Phe Phe Ala
130 135 140
Ala Ile Leu Gly Asn Lys Thr Ala Ile Ser Gly Gly Ser Gly Lys Val
145 150 155 160
Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His
165 170 175
Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Pro Tyr Ile Tyr Ala
180 185 190
Asp Arg Leu Ile Glu Val Leu Lys Lys Lys His Ala Ala Gly Thr Tyr
195 200 205
Glu Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe
210 215 220
Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Phe Ala Thr Thr Ala Ser
225 230 235 240
Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro
245 250 255
Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val
260 265 270
Ala Trp Met Glu Asp Ser Asp Ile His Asn Leu Arg Ser Glu Thr Leu
275 280 285
His Gln Gln Tyr Glu Leu Val Lys Ser Arg Thr Ala Ser Asp Asn Ser
290 295 300
Pro Tyr Gly Ser His Val Met Gln Tyr Gly Asp Ile Pro Leu Ser Lys
305 310 315 320
Asn Asn Leu Phe Met Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Tyr
325 330 335
Thr Phe Met Pro Gln Asn Phe Leu Arg Pro Ser Ser Ser Lys Ala Val
340 345 350
Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp His Lys Tyr Arg Lys
355 360 365
Ala Pro Glu Gly Ser Ala Arg Lys Ala Gln Ala Gln Lys Glu Phe Leu
370 375 380
Glu Ala Met Ser His Arg Met His Ile Asp Glu Ser Val Lys Leu Ile
385 390 395 400
Gly Lys Leu Leu Phe Gly Ile Lys Lys Gly Pro Glu Val Leu Ser Ala
405 410 415
Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys
420 425 430
Thr Met Val Arg Ser Phe Glu Thr Tyr Cys Gly Ser Leu Ser Gln Tyr
435 440 445
Gly Met Lys His Met Arg Ser Leu Ala Asn Ile Cys Asn Ala Gly Met
450 455 460
Thr Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Val Asn Val
465 470 475 480
Pro Ser Gly Arg Trp Ser Ser Leu His Arg Gly Phe Ser Ala
485 490
<210> 36
<211> 482
<212> PRT
<213> Solanum tuberosum
<400> 36
Met Asn Arg Ser Ile Ala Gly Val Leu Phe Leu Ile Ala Leu Ser Leu
1 5 10 15
Asn Val Ser Val Ser Glu Ser Arg Asn Phe Leu Lys Leu Pro Ser Glu
20 25 30
Gly Ser Arg Phe Phe Asp Ala Asp Glu Asn Asp Ser Val Gly Thr Arg
35 40 45
Trp Ala Ile Leu Leu Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His
50 55 60
Gln Ala Asp Ile Cys His Ala Tyr Gln Leu Leu Lys Lys Gly Gly Leu
65 70 75 80
Lys Asp Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Asn Asn
85 90 95
Glu Glu Asn Pro Arg Gln Gly Val Ile Ile Asn Ser Pro His Gly Glu
100 105 110
Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Thr
115 120 125
Val Asn Asn Phe Leu Ala Ala Leu Leu Gly Asn Lys Thr Ala Ile Thr
130 135 140
Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe
145 150 155 160
Ile Phe Tyr Ser Asp His Gly Gly Ala Gly Val Ile Gly Met Pro Thr
165 170 175
Asp Pro Tyr Leu Tyr Ala Asn Asp Leu Ile Asp Ala Leu Lys Lys Lys
180 185 190
His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys
195 200 205
Glu Ser Gly Ser Met Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile
210 215 220
TyrAla Thr Thr Ala Ser Asn Ala Asp Glu Ser Ser Trp Gly Thr Tyr
225 230 235 240
Cys Pro Gly Glu Phe Pro Ser Pro Pro Ile Glu Tyr Gly Thr Cys Leu
245 250 255
Gly Asp Leu Tyr Ser Ile Ser Trp Met Glu Asp Ser Glu Arg His Asn
260 265 270
Leu Arg Thr Glu Thr Leu Lys Gln Gln Tyr His Leu Val Lys Glu Arg
275 280 285
Thr Ala Ser Gly Asn Pro Ala Tyr Gly Ser His Val Met Gln Tyr Gly
290 295 300
Asp Val His Leu Ser Lys Asp Val Leu Phe Leu Tyr Met Gly Thr Asp
305 310 315 320
Pro Ala Asn Asp Asn Ser Thr Phe Met Asp Asp Asn Ser Met Arg Val
325 330 335
Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp Tyr
340 345 350
Lys Phe His Lys Ala Pro Glu Gly Ser Val Arg Lys Thr Glu Ala Gln
355 360 365
Lys Gln Leu Asn Glu Ala Ile Ser His Arg Met His Leu Asp Asn Ser
370 375 380
Ile Ala Leu Val Gly Lys Leu Leu Phe Gly Ile Lys Lys Gly Pro Glu
385 390 395 400
Val Leu Thr Ser Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp
405 410 415
Asp Cys Leu Lys Ser Tyr Val Arg Thr Phe Glu Thr His Cys Gly Ser
420 425 430
Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Val Ala Asn Ile Cys
435 440 445
Asn Ala Gly Ile Lys Met Glu Gln Met Val Glu Ala Ser Ala Gln Ala
450 455 460
Cys Pro Ser Val Pro Ser Asn Thr Trp Ser Ser Leu Gln Arg Gly Phe
465 470 475 480
Ser Ala
<210> 37
<211> 482
<212> PRT
<213> Solanum tuberosum
<400> 37
Met Asn Arg Ser Ile Ala Gly Val Leu Phe Leu Ile Ala Leu Ser Leu
1 5 10 15
Asn Val Ser Val Ser Glu Ser Arg Asn Phe Leu Lys Leu Pro Ser Glu
20 25 30
Gly Ser Arg Phe Phe Asp Ala Asp Glu Asn Asp Ser Val Gly Thr Arg
35 40 45
Trp Ala Ile Leu Leu Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His
50 55 60
Gln Ala Asp Ile Cys His Ala Tyr Gln Leu Leu Lys Lys Gly Gly Leu
65 70 75 80
Lys Asp Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Asn Asn
85 90 95
Glu Glu Asn Pro Arg Gln Gly Val Ile Ile Asn Ser Pro His Gly Glu
100 105 110
Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Thr
115 120 125
Val Asn Asn Phe Leu Ala Ala Leu Leu Gly Asn Lys Thr Ala Ile Thr
130 135 140
Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe
145 150 155 160
Ile Phe Tyr Ser Asp His Gly Gly Ala Gly Val Ile Gly Met Pro Thr
165 170 175
Asp Pro Tyr Leu Tyr Ala Asn Asp Leu Ile Asp Ala Leu Lys Lys Lys
180 185 190
His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys
195 200 205
Glu Ser Gly Ser Met Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile
210 215 220
Tyr Ala Thr Thr Ala Ser Asn Ala Asp Glu Ser Ser Trp Gly Thr Tyr
225 230 235 240
Cys Pro Gly Glu Phe Pro Ser Pro Pro Ile Glu Tyr Gly Thr Cys Leu
245 250 255
Gly Asp Leu Tyr Ser Ile Ser Trp Met Glu Asp Ser Gly Arg His Asn
260 265 270
Leu Arg Thr Glu Thr Leu Lys Gln Gln Tyr His Leu Val Lys Glu Arg
275 280 285
Thr Ala Ser Gly Asn Pro Ala Tyr Gly Ser His Val Met Gln Tyr Gly
290 295 300
Asp Val His Leu Ser Lys Asp Val Leu Phe Leu Tyr Met Gly Thr Asp
305 310 315 320
Pro Ala Asn Asp Asn Ser Thr Phe Met Asp Asp Asn Ser Met Arg Val
325 330 335
Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp Tyr
340 345 350
Lys Phe His Lys Ala Pro Glu Gly Ser Val Arg Lys Thr Glu Ala Gln
355 360 365
Lys Gln Leu Asn Glu Ala Ile Ser His Arg Met His Leu Asp Asn Ser
370 375 380
Ile Ala Leu Val Gly Lys Leu Leu Phe Gly Ile Lys Lys Gly Pro Glu
385 390 395 400
Val Leu Thr Ser Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp
405 410 415
Asp Cys Leu Lys Ser Tyr Val Arg Thr Phe Glu Thr His Cys Gly Ser
420 425 430
Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Val Ala Asn Ile Cys
435 440 445
Asn Ala Gly Ile Lys Met Glu Gln Met Val Glu Ala Ser Ala Gln Ala
450 455 460
Cys Pro Ser Val Pro Ser Asn Thr Trp Ser Ser Leu Gln Arg Gly Phe
465 470 475 480
Ser Ala
<210> 38
<211> 454
<212> PRT
<213> Glycine max
<400> 38
Met Asp Arg Phe Pro Ile Leu Phe Leu Leu Ala Thr Leu Ile Thr Leu
1 5 10 15
Ala Ser Gly Ala Arg His Asp Ile Leu Arg Leu Pro Ser Glu Ala Ser
20 25 30
Thr Phe Phe Lys Ala Pro Gly Gly Asp Gln Asn Asp Glu Gly Thr Arg
35 40 45
Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His
50 55 60
Gln Ser Asp Val Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu
65 70 75 80
Lys Glu Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Phe Asn
85 90 95
Glu Glu Asn Pro Arg Pro Gly Val Ile Ile Asn Ser Pro His Gly Asn
100 105 110
Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Ile Gly Glu Asp Val Thr
115 120 125
Val Gly Asn Phe Phe Ala Ala Ile Leu Gly Asn Lys Ser Ala Leu Thr
130 135 140
Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe
145 150 155 160
Ile Tyr Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr
165 170 175
Asn Pro Tyr Met Tyr Ala Ser Asp Leu Ile Glu Val Leu Lys Lys Lys
180 185 190
His Ala Ser Gly Ser Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys
195 200 205
Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile
210 215 220
Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr
225 230 235 240
Cys Pro Gly Glu Tyr Pro Ser Pro Pro Ser Glu Tyr Glu Thr Cys Leu
245 250 255
Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Ile His Asn
260 265 270
Leu Gln Thr Glu Thr Leu His Gln Gln Tyr Glu Leu Val Lys Gln Arg
275 280 285
Thr Met Asn Gly Asn Ser Ile Tyr Gly Ser His Val Met Gln Tyr Gly
290 295 300
Asp Ile Gly Leu Ser Glu Asn Asn Leu Val Leu Tyr Leu Asp Leu Ile
305 310 315 320
His Phe Trp Asp Lys Phe Arg Lys Ala Pro Val Gly Ser Ser Arg Lys
325 330 335
Ala Ala Ala Glu Lys Gln Ile Leu Glu Ala Met Ser His Arg Met His
340 345 350
Ile Asp Asp Ser Met Lys Arg Ile Gly Lys Leu Phe Phe Gly Ile Glu
355 360 365
Lys Gly Pro Glu Leu Leu Ser Ser Val Arg Pro Ala Gly Gln Pro Leu
370 375 380
Val Asp Asp Trp Asp Cys Leu Lys Thr Leu Val Arg Thr Phe Glu Thr
385 390 395 400
His Cys Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Phe
405 410 415
Ala Asn Phe Cys Asn Ala Gly Ile Arg Lys Glu Gln Met Ala Glu Ala
420 425 430
Ser Ala Gln Ala Cys Val Asn Ile Pro Ala Ser Ser Trp Ser Ser Met
435 440 445
His Arg Gly Phe Ser Ala
450
<210> 39
<211> 490
<212> PRT
<213> Papaver rhoeas
<400> 39
Met Val Lys Phe Leu Phe Ser Val Ile Ile Leu Phe Phe Leu Leu Ser
1 5 10 15
Ala Val Gly Ser Ser Ala Arg Asn Ile Glu Glu Asp Gly Val Ile Arg
20 25 30
Leu Pro Ser Glu Val Lys Asp Phe Ile Asn Gly Lys Asn Ile Asp Asp
35 40 45
Asp Ser Val Gly Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Ser
50 55 60
Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln
65 70 75 80
Val Leu Lys Arg Gly Gly Val Lys Asp Glu Asn Ile Val Val Phe Met
85 90 95
Tyr Asp Asp Ile Ala Leu Asn Glu Glu Asn Pro Arg Pro Gly Val Ile
100 105 110
Ile Asn His Pro Lys Gly Glu Asp Val Tyr Ala Gly Val Pro Lys Asp
115 120 125
Tyr Thr Gly Arg Asp Val Thr Ala His Asn Phe Tyr Ser Val Leu Leu
130 135 140
Gly Asn Lys Thr Ala Val Lys Gly Gly Ser Gly Lys Val Ile Asp Ser
145 150 155 160
Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His Gly Gly Pro
165 170 175
Gly Val Leu Gly Met Pro Thr Tyr Pro Tyr Leu Tyr Ala Asp Asp Leu
180 185 190
Val Asn Val Leu Lys Gln Lys His Ala Leu Gly Ala Tyr Lys Ser Leu
195 200 205
Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Ile
210 215 220
Leu Pro Lys Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu
225 230 235 240
Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Phe Pro Ser Pro Pro
245 250 255
Ser Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met
260 265 270
Glu Asp Ser Asp Val His Asn Leu Arg Ser Glu Thr Leu Lys Gln Gln
275 280 285
Tyr His Leu Val Lys Glu Arg Thr Gln Asn Ala Asn Ser Ala Tyr Gly
290 295 300
Ser His Val Met Gln Tyr Gly Asp Leu Glu Val Ser Lys Glu Asp Leu
305 310 315 320
Phe Leu Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Asn Lys Phe Ile
325 330 335
Glu Gln Asn Ser Leu Pro Ser Leu Ser Gly Ser Val Asn Gln Arg Glu
340 345 350
Ala Asp Leu Ile His Phe Trp Gln Lys Tyr Arg Lys Ala Pro Glu Gly
355 360 365
Ser Gln Arg Lys Ala Asp Ala Gln Lys Gln Phe Val Glu Val Met Ala
370 375 380
His Arg Met His Val Asp His Ser Ile Lys Leu Ile Gly Lys Leu Leu
385 390 395 400
Phe Gly Phe Glu Lys Gly Pro Gln Val Leu Glu Ala Val Arg Pro Ala
405 410 415
Gly Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Thr Met Val Arg
420 425 430
Thr Phe Glu Ala Gln Cys Gly Ser Leu Ser Gln Tyr Gly Met Lys His
435 440 445
Met Arg Ser Val Ala Asn Ile Cys Asn Ala Gly Ile Lys Lys Glu Gln
450 455 460
Met Ala Glu Ala Ala Ser Gln Ala Cys Val Thr Ile Pro Asn Gly Ser
465 470 475 480
Trp Ser Ser Thr His Gln Gly Phe Ser Ala
485 490
<210> 40
<211> 483
<212> PRT
<213> Vigna radiata
<400> 40
Met Ala Thr Thr Thr Thr Ser Leu Ser Thr Leu Phe Leu Leu Phe Leu
1 5 10 15
Ala Thr Val Ala Leu Val Ala Ala Gly Arg Asp Leu Val Gly Asp Phe
20 25 30
Leu Arg Leu Pro Ser Asp Ser Gly Asn Asp Asp Asn Val Lys Gly Thr
35 40 45
Arg Trp Ala Ile Leu Phe Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg
50 55 60
His Gln Ala Asp Ile Cys His Ala Tyr Gln Ile Leu Arg Lys Gly Gly
65 70 75 80
Leu Lys Glu Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Phe
85 90 95
Asn Trp Asp Asn Pro Arg Pro Gly Val Ile Ile Asn Lys Pro Asp Gly
100 105 110
Asp Asp Val Tyr Glu Gly Val Pro Lys Asp Tyr Thr Gly Glu Asp Ala
115 120 125
Thr Ala His Asn Phe Tyr Ser Ala Leu Leu Gly Asp Lys Ser Ala Leu
130 135 140
Thr Gly Gly Ser Gly Lys Val Val Asn Ser Gly Pro Asp Asp Arg Ile
145 150 155 160
Phe Ile Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Thr Pro
165 170 175
Ala Gly Pro Tyr Ile Tyr Ala Ser Asp Leu Val Glu Val Leu Lys Lys
180 185 190
Lys His Ala Ser Gly Thr Tyr Lys Asn Leu Val Phe Tyr Leu Glu Ala
195 200 205
Cys Glu Ala Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Asp Ile Asn
210 215 220
Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr
225 230 235 240
Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Ser Thr Cys
245 250 255
Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Arg His
260 265 270
Asn Leu Arg Thr Glu Ser Leu His Gln Gln Tyr Lys Val Val Lys Asp
275 280 285
Arg Thr Leu Ser Gly Gly Trp Tyr Gly Ser His Val Met Gln Tyr Gly
290 295 300
Asp Val Glu Phe Ser Lys Asp Thr Leu Phe Leu Tyr Leu Gly Thr Asp
305 310 315 320
Pro Ala Asn Asp Asn Leu Thr Phe Val Asp Glu Asn Ser Leu Trp Ser
325 330 335
Ser Ser Thr Ala Val Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp
340 345 350
His Lys Phe Arg Lys Ala Pro Glu Gly Ser Pro Lys Lys Asn Glu Ala
355 360 365
Arg Lys Gln Val Leu Glu Val Met Ser His Arg Met His Ile Asp Asp
370 375 380
Ser Val Lys Leu Val Gly Lys Leu Leu Phe Gly Phe Glu Lys Ala Pro
385 390 395 400
Glu Val Leu Asn Ala Val Arg Pro Ala Gly Ser Ala Leu Val Asp Asp
405 410 415
Trp Ala Cys Leu Lys Thr Met Val Arg Thr Phe Glu Thr His Cys Gly
420 425 430
Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Ile
435 440 445
Cys Asn Val Gly Ile Lys Lys Glu Gln Met Ala Glu Ala Ser Ala Gln
450 455 460
Ala Cys Val Thr Val Pro Ala Ser Ser Trp Ser Ser Leu Gln Arg Gly
465 470 475 480
Phe Ser Ala
<210> 41
<211> 489
<212> PRT
<213> Nicotiana tabacum
<400> 41
Met Ile Arg Tyr Val Ala Gly Thr Leu Phe Leu Ile Gly Leu Ala Leu
1 5 10 15
Asn Val Ala Val Ser Glu Ser Arg Asn Val Leu Lys Leu Pro Ser Glu
20 25 30
Val Ser Arg Phe Phe Gly Ala Asp Glu Ser Asn Ala Gly Asp His Asp
35 40 45
Asp Asp Ser Val Gly Thr Arg Trp Ala Ile Leu Leu Ala Gly Ser Asn
50 55 60
Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln
65 70 75 80
Leu Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Val Val Phe Met
85 90 95
Tyr Asp Asp Ile Ala Asn Asn Glu Glu Asn Pro Arg Arg Gly Val Ile
100 105 110
Ile Asn Ser Pro His Gly Glu Asp Val Tyr Lys Gly Val Pro Lys Asp
115 120 125
Tyr Thr Gly Asp Asp Val Thr Val Asp Asn Phe Phe Ala Val Ile Leu
130 135 140
Gly Asn Lys Thr Ala Leu Ser Gly Gly Ser Gly Lys Val Val Asn Ser
145 150 155 160
Gly Pro Asn Asp His Ile Phe Ile Phe Tyr Ser Asp His Gly Gly Pro
165 170 175
Gly Val Leu Gly Met Pro Thr Asp Pro Tyr Leu Tyr Ala Asn Asp Leu
180 185 190
Ile Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu
195 200 205
Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu
210 215 220
Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu
225 230 235 240
Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro
245 250 255
Ile Glu Tyr Met Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ser Trp Met
260 265 270
Glu Asp Ser Glu Leu His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln
275 280 285
Tyr His Leu Val Lys Glu Arg Thr Ala Thr Gly Asn Pro Val Tyr Gly
290 295 300
Ser His Val Met Gln Tyr Gly Asp Leu His Leu Ser Lys Asp Ala Leu
305 310 315 320
Tyr Leu Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Tyr Thr Phe Met
325 330 335
Asp Asp Asn Ser Leu Arg Val Ser Lys Ala Val Asn Gln Arg Asp Ala
340 345 350
Asp Leu Leu His Phe Trp His Lys Phe Arg Thr Ala Pro Glu Gly Ser
355 360 365
Val Arg Lys Ile Glu Ala Gln Lys Gln Leu Asn Glu Ala Ile Ser His
370 375 380
Arg Val His Leu Asp Asn Ser Val Ala Leu Val Gly Lys Leu Leu Phe
385 390 395 400
Gly Ile Glu Lys Gly Pro Glu Val Leu Ser Gly Val Arg Pro Ala Gly
405 410 415
Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Ser Phe Val Arg Thr
420 425 430
Phe Glu Thr His Cys Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met
435 440 445
Arg Ser Ile Ala Asn Ile Cys Asn Ala Gly Ile Lys Lys Glu Gln Met
450 455 460
Val Glu Ala Ser Ala Gln Ala Cys Pro Ser Val Pro Ser Asn Thr Trp
465 470 475 480
Ser Ser Leu His Arg Gly Phe Ser Ala
485
<210> 42
<211> 482
<212> PRT
<213> Solanum lycopersicum
<400> 42
Met Asn Arg Ser Val Ala Gly Val Leu Phe Leu Ile Ala Leu Ser Leu
1 5 10 15
Asn Val Ser Val Ser Glu Ser Arg Asn Phe Leu Lys Leu Pro Ser Glu
20 25 30
Gly Ser Arg Phe Phe Asp Ala Asp Glu Ile Asp Ser Val Gly Thr Arg
35 40 45
Trp Ala Ile Leu Leu Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His
50 55 60
Gln Ala Asp Ile Cys His Ala Tyr Gln Leu Leu Lys Lys Gly Gly Leu
65 70 75 80
Lys Asp Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Asn Asn
85 90 95
Glu Glu Asn Pro Arg Gln Gly Val Ile Ile Asn Ser Pro His Gly Glu
100 105 110
Asp Val Tyr Asn Gly Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Thr
115 120 125
Val Asp Asn Phe Leu Ala Ala Leu Leu Gly Asn Lys Thr Ala Leu Thr
130 135 140
Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe
145 150 155 160
Ile Phe Tyr Ser Asp His Gly Gly Ala Gly Val Leu Gly Met Pro Thr
165 170 175
Asn Pro Tyr Leu Tyr Ala Asn Asp Leu Ile Asp Ala Leu Lys Met Lys
180 185 190
His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys
195 200 205
Glu Ser Gly Ser Met Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile
210 215 220
Tyr Ala Thr Thr Ala Ser Asn Ala Asp Glu Ser Ser Trp Gly Thr Tyr
225 230 235 240
Cys Pro Gly Glu Tyr Pro Ser Pro Pro Ile Glu Tyr Asp Thr Cys Leu
245 250 255
Gly Asp Leu Tyr Ser Ile Ser Trp Met Glu Asp Ser Glu Arg His Asn
260 265 270
Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr His Leu Val Lys Glu Arg
275 280 285
Thr Ala Ser Gly Asn Pro Ala Tyr Gly Ser His Val Met Gln Tyr Gly
290 295 300
Asp Val His Leu Ser Lys Asp Ala Val Phe Leu Tyr Met Gly Thr Asp
305 310 315 320
Pro Ala Asn Asp Asn Ser Thr Phe Met Asp Asp Asn Ser Leu Arg Val
325 330 335
Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp Tyr
340 345 350
Lys Phe His Lys Ala Pro Glu Gly Ser Val Ser Lys Thr Glu Ala Gln
355 360 365
Lys Arg Leu Asn Glu Ala Ile Ser His Arg Met His Leu Asp Asn Ser
370 375 380
Ile Ala Leu Val Gly Lys Leu Leu Phe Gly Ile Lys Lys Gly Pro Glu
385 390 395 400
Val Leu Thr Ser Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asn Trp
405 410 415
Asp Cys Leu Lys Ser Tyr Val Arg Thr Phe Glu Thr His Cys Gly Ser
420 425 430
Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Val Ala Asn Ile Cys
435 440 445
Asn Ala Gly Ile Lys Met Glu Gln Met Val Glu Ala Ser Ala Gln Ala
450 455 460
Cys Pro Ser Val Pro Ser Tyr Thr Trp Ser Ser Leu His Arg Gly Phe
465 470 475 480
Ser Ala
<210> 43
<211> 494
<212> PRT
<213> Citrus clementina
<400> 43
Met Thr Arg Leu Ala Ser Gly Val Leu Ile Thr Leu Leu Val Ala Leu
1 5 10 15
Ala Gly Ile Ala Asp Gly Ser Arg Asp Ile Ala Gly Asp Ile Leu Lys
20 25 30
Leu Pro Ser Glu Ala Tyr Arg Phe Phe His Asn Gly Gly Gly Gly Ala
35 40 45
Lys Val Asn Asp Asp Asp Asp Ser Val Gly Thr Arg Trp Ala Val Leu
50 55 60
Leu Ala Gly Ser Asn Gly Phe Trp Asn Tyr Arg His Gln Ala Asp Ile
65 70 75 80
Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Asp Glu Asn
85 90 95
Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Phe Asn Glu Glu Asn Pro
100 105 110
Arg Pro Gly Val Ile Ile Asn His Pro His Gly Asp Asp Val Tyr Lys
115 120 125
Gly Val Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val Glu Asn Phe
130 135 140
Phe Ala Val Ile Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser Gly
145 150 155 160
Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Phe Tyr Ser
165 170 175
Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Arg Tyr Ile
180 185 190
Tyr Ala Asp Glu Leu Ile Asp Val Leu Lys Lys Lys His Ala Ser Gly
195 200 205
Asn Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser
210 215 220
Ile Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr
225 230 235 240
Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu
245 250 255
Ile Pro Gly Pro Pro Pro Glu Tyr Ser Thr Cys Leu Gly Asp Leu Tyr
260 265 270
Ser Ile Ala Trp Met Glu Asp Ser Asp Ile His Asn Leu Arg Thr Glu
275 280 285
Thr Leu His Gln Gln Tyr Glu Leu Val Lys Thr Arg Thr Ala Ser Tyr
290 295 300
Asn Ser Tyr Gly Ser His Val Met Gln Tyr Gly Asp Ile Gly Leu Ser
305 310 315 320
Lys Asn Asn Leu Phe Thr Tyr Leu Gly Thr Asn Pro Ala Asn Asp Asn
325 330 335
Tyr Thr Phe Val Asp Glu Asn Ser Leu Arg Pro Ala Ser Lys Ala Val
340 345 350
Asn Gln Arg Asp Ala Asp Leu Leu His Phe Trp Asp Lys Tyr Arg Lys
355 360 365
Ala Pro Glu Gly Thr Pro Arg Lys Ala Glu Ala Gln Lys Gln Phe Phe
370 375 380
Glu Ala Met Ser His Arg Met His Val Asp His Ser Ile Lys Leu Ile
385 390 395 400
Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly Pro Glu Ile Leu Asn Thr
405 410 415
Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Gly Cys Leu Lys
420 425 430
Ser Leu Val Arg Thr Phe Glu Ser His Cys Gly Ala Leu Ser Gln Tyr
435 440 445
Gly Met Lys His Met Arg Ser Leu Ala Asn Ile Cys Asn Thr Gly Ile
450 455 460
Gly Lys Glu Lys Met Ala Glu Ala Ser Ala Gln Ala Cys Glu Asn Ile
465 470 475 480
Pro Ser Gly Pro Trp Ser Ser Leu Asp Lys Gly Phe Ser Ala
485 490
<210> 44
<211> 481
<212> PRT
<213> Nicotiana tabacum
<400> 44
Met Ile Ser Tyr Ala Ala Gly Ile Phe Phe Leu Val Gly Phe Ser Ile
1 5 10 15
Ala Ala Ala Ala Asp Gly Arg Asn Val Leu Lys Leu Pro Ser Glu Ala
20 25 30
Ser Arg Phe Phe Asp Glu Ala Asp Asp Ser Val Gly Thr Arg Trp Ala
35 40 45
Val Leu Leu Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala
50 55 60
Asp Val Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Asp
65 70 75 80
Glu Asn Ile Ile Met Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Glu
85 90 95
Asn Pro Arg Gln Gly Val Ile Ile Asn Ser Pro Ala Gly Glu Asp Val
100 105 110
Tyr Lys Gly Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Asn Val Asp
115 120 125
Asn Phe Leu Ala Val Leu Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly
130 135 140
Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Phe
145 150 155 160
Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Asn Pro
165 170 175
Tyr Leu Tyr Ala Ser Asp Leu Ile Asp Val Leu Lys Lys Lys His Ala
180 185 190
Ser Gly Thr Tyr Lys Ser Leu Val Leu Tyr Ile Glu Ala Cys Glu Ser
195 200 205
Gly Ser Ile Phe Glu Gly Leu Leu Pro Lys Gly Leu Asn Ile Tyr Ala
210 215 220
Thr Thr Ala Ser Asn Ala Val Glu Ser Ser Trp Gly Thr Tyr Cys Pro
225 230 235 240
Gly Asp Tyr Pro Ser Leu Pro Pro Gly Tyr Glu Thr Cys Leu Gly Asp
245 250 255
Leu Tyr Ala Val Ser Trp Met Glu Asp Ser Glu Met His Asn Leu Arg
260 265 270
Thr Glu Asn Leu Arg Gln Gln Tyr His Leu Val Lys Glu Arg Thr Ala
275 280 285
Asn Gly Asn Ser Ala Tyr Gly Ser His Val Leu Gln Phe Gly Asp Leu
290 295 300
Gln Leu Gly Met Asp Ser Leu Phe Met Tyr Met Gly Thr Asn Pro Ala
305 310 315 320
Asn Asp Asn Tyr Thr Tyr Val Asp Asp Asn Ser Leu Arg Ala Ser Ser
325 330 335
Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Leu His Phe Trp Asp Lys
340 345 350
Phe Arg Lys Ala Pro Glu Gly Ser Ala Arg Lys Val Glu Ala Gln Lys
355 360 365
Gln Phe Thr Glu Ala Met Ser His Arg Met His Leu Asp Asn Ser Met
370 375 380
Ala Leu Val Gly Lys Leu Leu Phe Gly Ile Gln Lys Gly Pro Glu Val
385 390 395 400
Leu Lys Arg Val Arg Pro Val Gly Gln Pro Leu Val Asp Asp Trp Thr
405 410 415
Cys Leu Lys Tyr Phe Val Arg Thr Phe Glu Thr His Cys Gly Ser Leu
420 425 430
Ser Gln Tyr Gly Met Lys His Met Arg Ser Ile Ala Asn Ile Cys Asn
435 440 445
Ala Gly Ile Lys Met Glu Gln Met Val Glu Ala Ser Thr Gln Ala Cys
450 455 460
Pro Ser Val Pro Thr Asn Ile Trp Ser Ser Leu His Arg Gly Phe Ser
465 470 475 480
Ala
<210> 45
<211> 481
<212> PRT
<213> Capsella rubella
<400> 45
Met Thr Arg Ile Val Ala Val Ile Val Val Val Leu Phe Leu Ser Leu
1 5 10 15
Val Ala Ala Ala Ser Asp Asn Phe Ile Arg Leu Pro Ser Glu Ala Ser
20 25 30
Lys Phe Phe Arg Pro Asn Asn Glu Asn Asp Asp Ser Thr Arg Trp Ala
35 40 45
Val Leu Val Ala Gly Ser Ser Gly Tyr Trp Asn Tyr Arg His Gln Ala
50 55 60
Asp Val Cys His Ala Tyr Gln Leu Leu Lys Lys Gly Gly Val Lys Glu
65 70 75 80
Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Asn Asn Glu Glu
85 90 95
Asn Pro Arg Pro Gly Val Ile Ile Asn Ser Pro Asn Gly Glu Asp Val
100 105 110
Tyr Asn Gly Val Pro Lys Asp Tyr Thr Gly Asp Glu Val Asn Val Asn
115 120 125
Asn Phe Phe Ala Val Leu Leu Gly Asn Lys Thr Ala Leu Lys Gly Gly
130 135 140
Ser Gly Lys Val Val Asn Ser Gly Pro Asn Asp His Ile Phe Ile Tyr
145 150 155 160
Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Pro
165 170 175
Tyr Leu Tyr Ala Lys Asp Leu Asn Asp Val Leu Lys Lys Lys His Ala
180 185 190
Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser
195 200 205
Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala
210 215 220
Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro
225 230 235 240
Gly Glu Asp Pro Ser Pro Pro Ser Glu Tyr Glu Thr Cys Leu Gly Asp
245 250 255
Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Lys His Asn Leu Gln
260 265 270
Thr Glu Ser Leu His Gln Gln Tyr Glu Leu Val Lys Lys Arg Thr Ala
275 280 285
Gly Thr Gly Ser Ser Tyr Gly Ser His Val Leu Glu Phe Gly Asp Ile
290 295 300
Gly Leu Ser Lys Glu Lys Leu Val Leu Tyr Met Gly Thr Asn Pro Ala
305 310 315 320
Asn Glu Asn Phe Thr Phe Val Asp Glu Asn Ser Ser Leu Arg Leu Pro
325 330 335
Ser Arg Val Thr Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp Asp
340 345 350
Lys Tyr Arg Lys Ala Pro Glu Gly Ser Ala Arg Lys Val Glu Ala Gln
355 360 365
Lys Gln Val Leu Glu Ala Met Ser His Arg Leu His Val Asp Asn Ser
370 375 380
Val Leu Leu Ile Gly Lys Leu Leu Phe Gly Leu Glu Gly Pro Ala Val
385 390 395 400
Leu Asn Lys Val Arg Pro Ser Gly Arg Pro Leu Val Asp Asp Trp Asp
405 410 415
Cys Leu Lys Ser Met Val Arg Ala Phe Glu Arg His Cys Gly Ser Leu
420 425 430
Ser Gln Tyr Gly Ile Lys His Met Arg Ser Ile Ala Asn Ile Cys Asn
435 440 445
Ala Gly Ile Gln Met Gly Leu Met Glu Glu Ala Ala Lys Gln Ala Cys
450 455 460
Pro Ser Ile Pro Ala Gly Pro Trp Ser Ser Leu His Arg Gly Phe Ser
465 470 475 480
Ala
<210> 46
<211> 479
<212> PRT
<213> Arabidopsis lyrata subsp. lyrata
<400> 46
Met Thr Thr Val Ala Val Thr Phe Leu Ala Leu Phe Leu Tyr Leu Val
1 5 10 15
Ala Ala Val Ser Gly Asp Val Ile Lys Leu Pro Ser Gln Ala Ser Lys
20 25 30
Phe Phe His Pro Thr Glu Asn Asp Asp Asp Ser Thr Arg Trp Ala Val
35 40 45
Leu Val Ala Gly Ser Ser Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp
50 55 60
Val Cys His Ala Tyr Gln Leu Leu Lys Lys Gly Gly Val Lys Glu Glu
65 70 75 80
Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Lys Asn Glu Glu Asn
85 90 95
Pro Arg Pro Gly Val Ile Ile Asn Ser Pro Asn Gly Glu Asp Val Tyr
100 105 110
Asn Gly Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Asn Val Asp Asn
115 120 125
Leu Leu Ala Val Ile Leu Gly Asn Lys Thr Ala Val Lys Gly Gly Ser
130 135 140
Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr
145 150 155 160
Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Pro Tyr
165 170 175
Leu Tyr Ala Asn Asp Leu Asn Asp Val Leu Lys Lys Lys His Ala Ser
180 185 190
Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly
195 200 205
Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr
210 215 220
Thr Ala Ser Asn Ala Val Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly
225 230 235 240
Glu Asp Pro Ser Pro Pro Ser Glu Tyr Glu Thr Cys Leu Gly Asp Leu
245 250 255
Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Ile His Asn Leu Gln Thr
260 265 270
Glu Thr Leu His Gln Gln Tyr Glu Leu Val Lys Lys Arg Thr Ala Gly
275 280 285
Ser Gly Lys Ser Phe Gly Ser His Val Met Glu Phe Gly Asp Ile Gly
290 295 300
Leu Ser Lys Glu Lys Leu Val Leu Tyr Met Gly Thr Asn Pro Ala Asn
305 310 315 320
Glu Asn Phe Thr Phe Val Asn Glu Asn Ser Leu Arg Pro Pro Ser Arg
325 330 335
Val Thr Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp Asp Lys Tyr
340 345 350
Arg Lys Ala Pro Glu Gly Ser Ala Arg Lys Val Glu Ala Gln Lys Gln
355 360 365
Val Leu Glu Ala Met Ser His Arg Leu His Val Asp Asn Ser Ile Leu
370 375 380
Leu Ile Gly Lys Leu Leu Phe Gly Leu Asp Ser Pro Ala Val Leu Asn
385 390 395 400
Asn Val Arg Pro Ser Gly Thr Pro Leu Val Asp Asp Trp Asp Cys Leu
405 410 415
Lys Ser Leu Val Arg Val Phe Glu Met His Cys Gly Ser Leu Ser Gln
420 425 430
Tyr Gly Ile Lys His Met Arg Ser Ile Ala Asn Ile Cys Asn Ala Gly
435 440 445
Ile Gln Met Gly Gln Met Glu Glu Ala Ala Met Gln Ala Cys Pro Thr
450 455 460
Ile Pro Ala Ser Pro Trp Ser Ser Leu Glu Arg Gly Phe Ser Ala
465 470 475
<210> 47
<211> 482
<212> PRT
<213> Capsella rubella
<400> 47
Met Thr Arg Leu Thr Val Gly Val Leu Phe Leu Ser Leu Ile Ala Leu
1 5 10 15
Ser Ala Ala Arg Asn Gly Pro Asp Asp Val Ile Lys Leu Pro Ser Gln
20 25 30
Ala Ser Arg Phe Phe Arg Pro Pro Glu Asn Asp Ala Gly Thr Arg Trp
35 40 45
Ala Val Leu Val Ala Gly Ser Ser Gly Tyr Gly Asn Tyr Arg His Gln
50 55 60
Ala Asp Ile Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys
65 70 75 80
Glu Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Asn Asn Tyr
85 90 95
Glu Asn Pro Arg Pro Gly Thr Ile Ile Asn Ser Pro His Gly Lys Asp
100 105 110
Val Tyr Gln Gly Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Thr Val
115 120 125
Asp Asn Leu Phe Ala Val Ile Leu Gly Asp Lys Thr Ala Ile Lys Gly
130 135 140
Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile
145 150 155 160
Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Phe
165 170 175
Pro Tyr Ile Tyr Ala Asn Asp Leu Asn Asp Val Leu Lys Lys Lys His
180 185 190
Ala Leu Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu
195 200 205
Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr
210 215 220
Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys
225 230 235 240
Pro Gly Glu Glu Pro Ser Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly
245 250 255
Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Gly Ile His Asn Leu
260 265 270
Gln Thr Glu Thr Leu Gln Gln Gln Tyr Glu Leu Val Lys Lys Arg Thr
275 280 285
Ala Leu Asp Gly Tyr Tyr Tyr Gly Ser His Val Met Gln Tyr Gly Asp
290 295 300
Val Gly Leu Ser Lys Asp Lys Leu Glu Ile Tyr Met Gly Thr Asn Pro
305 310 315 320
Ala Asn Glu Asn Ser Thr Phe Val Asp Ser Asn Ser Leu Lys Leu Pro
325 330 335
Ser Arg Val Thr Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp Glu
340 345 350
Lys Tyr Arg Lys Ala Pro Glu Gly Ser Ser Arg Lys Ala Glu Ala Gln
355 360 365
Lys Gln Val Leu Glu Val Met Ser His Arg Leu His Val Asp Asn Ser
370 375 380
Val Ile Leu Val Gly Lys Ile Leu Phe Gly Ile Ser Lys Gly Pro Gln
385 390 395 400
Val Leu Asn Glu Val Arg Ser Ala Gly Gln Pro Leu Val Asp Asp Trp
405 410 415
Asn Cys Leu Lys Asn Met Val Arg Ala Phe Glu Arg His Cys Gly Ser
420 425 430
Leu Ser Gln Tyr Gly Ile Lys His Met Arg Ser Phe Ala Asn Phe Cys
435 440 445
Asn Ser Gly Ile Gln Met Glu Gln Met Glu Glu Ala Ala Ser Gln Ala
450 455 460
Cys Thr Thr Ile Pro Pro Gly Pro Trp Ser Ser Leu His Arg Gly Phe
465 470 475 480
Ser Ala
<210> 48
<211> 482
<212> PRT
<213> Vigna mungo
<400> 48
Met Ala Thr Thr Thr Ser Leu Ser Thr Leu Phe Leu Leu Phe Leu Ala
1 5 10 15
Thr Val Ala Leu Val Ala Ala Arg Arg Asp His Val Gly Asp Phe Leu
20 25 30
Arg Leu Pro Ser Asp Ser Gly Asn Asp Asp Asn Val Gln Gly Thr Arg
35 40 45
Trp Ala Ile Leu Phe Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His
50 55 60
Gln Ala Asp Ile Cys His Ala Tyr Gln Ile Leu Arg Lys Gly Gly Leu
65 70 75 80
Lys Glu Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Phe Asn
85 90 95
Trp Asp Asn Pro Arg Pro Gly Val Ile Ile Asn Lys Pro Asp Gly Asp
100 105 110
Asp Val Tyr Glu Gly Val Pro Lys Asp Tyr Thr Gly Glu Asp Ala Thr
115 120 125
Ala His Asn Phe Tyr Ser Ala Leu Leu Gly Asp Lys Ser Ala Leu Thr
130 135 140
Gly Gly Ser Gly Lys Val Val Ser Ser Gly Pro Asp Asp Arg Ile Phe
145 150 155 160
Ile Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Thr Pro Ala
165 170 175
Gly Pro Tyr Ile Tyr Ala Ser Asp Leu Val Glu Val Leu Lys Lys Lys
180 185 190
His Ala Ser Gly Thr Tyr Lys Asn Leu Val Phe Tyr Leu Glu Ala Cys
195 200 205
Glu Ala Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Asp Ile Asn Ile
210 215 220
Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr
225 230 235 240
Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Ser Thr Cys Leu
245 250 255
Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Arg His Asn
260 265 270
Leu Arg Thr Glu Ser Leu His Gln Gln Tyr Lys Val Val Lys Asp Arg
275 280 285
Thr Leu Ser Gly Gly Trp Tyr Gly Ser His Val Met Gln Tyr Gly Asp
290 295 300
Val Glu Phe Ser Lys Asp Ala Leu Phe Leu Tyr Leu Gly Thr Asp Pro
305 310 315 320
Ala Asn Asp Asn Leu Thr Phe Val Asp Glu Asn Ser Leu Trp Ser Ser
325 330 335
Ser Thr Ala Val Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp His
340 345 350
Lys Phe Arg Lys Ala Pro Glu Gly Ser Pro Lys Lys Asn Glu Ala Arg
355 360 365
Lys Gln Val Leu Glu Val Met Ser His Arg Met His Ile Asp Asp Ser
370 375 380
Val Lys Leu Val Gly Lys Leu Leu Phe Gly Phe Glu Lys Ala Pro Glu
385 390 395 400
Val Leu Asn Ala Val Arg Pro Ala Gly Ser Ala Leu Val Asp Asp Trp
405 410 415
Ala Cys Leu Lys Thr Met Val Arg Thr Phe Glu Thr His Cys Gly Ser
420 425 430
Leu Ser Gln Tyr Gly Met Lys His Met Ser Pro Phe Ala Asn Ile Cys
435 440 445
Asn Val Gly Ile Lys Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala
450 455 460
Cys Val Thr Val Pro Ala Ser Ser Trp Ser Ser Leu Gln Arg Gly Phe
465 470 475 480
Ser Ala
<210> 49
<211> 488
<212> PRT
<213> Eutrema salsugineum
<400> 49
Met Thr Ser Val Ala Val Pro Leu Leu Val Leu Leu Leu Ser Leu Ile
1 5 10 15
Ala Val Ser Ala Ala Arg Gln Gly Pro Asp Asp Ile Ile Lys Leu Pro
20 25 30
Ser Gln Ala Ser Met Phe Phe Arg Pro Ala Asp Asp Asp Asn Asp Ser
35 40 45
Ser Ala Gly Thr Arg Trp Ala Val Leu Val Ala Gly Ser Asn Gly Tyr
50 55 60
Trp Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln Leu Leu
65 70 75 80
Arg Lys Gly Gly Val Lys Glu Asp Asn Ile Val Val Phe Met Tyr Asp
85 90 95
Asp Ile Ala Asn Asn Glu Glu Asn Pro Arg Arg Gly Ile Ile Ile Asn
100 105 110
Ser Pro His Gly Lys Asp Val Tyr Gln Gly Val Pro Lys Asp Tyr Thr
115 120 125
Gly Asp Asp Val Thr Val Asp Asn Leu Phe Ala Val Ile Leu Gly Asn
130 135 140
Lys Thr Ala Thr Lys Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro
145 150 155 160
Asn Asp His Ile Phe Ile Phe Tyr Ser Asp His Gly Gly Pro Gly Val
165 170 175
Leu Gly Met Pro Thr Ser Pro Tyr Leu Tyr Ala Asn Asp Leu Asn Asp
180 185 190
Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe
195 200 205
Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Glu
210 215 220
Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Val Glu Ser
225 230 235 240
Ser Trp Gly Thr Tyr Cys Pro Gly Glu Asp Pro Ser Leu Pro Pro Glu
245 250 255
Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val Ser Trp Met Glu Asp
260 265 270
Ser Gly Met His Asn Leu Gln Thr Glu Thr Leu Arg Gln Gln Tyr Glu
275 280 285
Leu Val Lys Arg Arg Thr Ala Gly Val Gly Ser Ala Tyr Gly Ser His
290 295 300
Val Met Gln Tyr Gly Asp Val Gly Leu Ser Lys Asp Lys Leu Asp Leu
305 310 315 320
Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Phe Thr Phe Val Asp Glu
325 330 335
Asn Ser Leu Thr Pro Pro Ser Arg Val Thr Asn Gln Arg Asp Ala Asp
340 345 350
Leu Val His Phe Trp Asp Lys Tyr Arg Lys Ala Pro Glu Gly Ser Thr
355 360 365
Arg Lys Thr Glu Ala Gln Lys Gln Val Leu Glu Ala Met Ser His Arg
370 375 380
Leu His Val Asp Asn Ser Val Lys Leu Val Gly Lys Leu Leu Phe Gly
385 390 395 400
Ile Ser Glu Gly Pro Glu Val Leu Asn Lys Val Arg Ser Ala Gly Gln
405 410 415
Pro Leu Val Asp Asp Trp Asn Cys Leu Lys Asn Leu Val Arg Ala Phe
420 425 430
Glu Arg His Cys Gly Ser Leu Ser Gln Tyr Gly Ile Lys His Met Arg
435 440 445
Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile Gln Met Glu Gln Met Glu
450 455 460
Glu Ala Ser Ser Gln Ala Cys Thr Thr Ile Pro Pro Gly Pro Trp Ser
465 470 475 480
Ser Leu His Arg Gly Phe Ser Ala
485
<210> 50
<211> 494
<212> PRT
<213> Citrus sinensis
<400> 50
Met Thr Arg Leu Ala Ser Gly Val Leu Ile Thr Leu Leu Val Ala Leu
1 5 10 15
Ala Gly Ile Ala Asp Gly Ser Arg Asp Ile Ala Gly Asp Ile Leu Lys
20 25 30
Leu Pro Ser Glu Ala Tyr Arg Phe Phe His Asn Gly Gly Gly Gly Ala
35 40 45
Lys Val Asn Asp Asp Asp Asp Ser Val Gly Thr Arg Trp Ala Val Leu
50 55 60
Leu Ala Gly Ser Asn Gly Phe Trp Asn Tyr Arg His Gln Ala Asp Ile
65 70 75 80
Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Asp Glu Asn
85 90 95
Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Phe Asn Glu Glu Asn Pro
100 105 110
Arg Pro Gly Val Ile Ile Asn His Pro His Gly Asp Asp Val Tyr Lys
115 120 125
Gly Val Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val Glu Lys Phe
130 135 140
Phe Ala Val Val Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser Gly
145 150 155 160
Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Phe Tyr Ser
165 170 175
Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Arg Tyr Ile
180 185 190
Tyr Ala Asp Glu Leu Ile Asp Val Leu Lys Lys Lys His Ala Ser Gly
195 200 205
Asn Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser
210 215 220
Ile Phe Glu Gly Leu Leu Leu Glu Gly Leu Asn Ile Tyr Ala Thr Thr
225 230 235 240
Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu
245 250 255
Ile Pro Gly Pro Pro Pro Glu Tyr Ser Thr Cys Leu Gly Asp Leu Tyr
260 265 270
Ser Ile Ala Trp Met Glu Asp Ser Asp Ile His Asn Leu Arg Thr Glu
275 280 285
Thr Leu His Gln Gln Tyr Glu Leu Val Lys Thr Arg Thr Ala Ser Tyr
290 295 300
Asn Ser Tyr Gly Ser His Val Met Gln Tyr Gly Asp Ile Gly Leu Ser
305 310 315 320
Lys Asn Asn Leu Phe Thr Tyr Leu Gly Thr Asn Pro Ala Asn Asp Asn
325 330 335
Tyr Thr Phe Val Asp Glu Asn Ser Leu Arg Pro Ala Ser Lys Ala Val
340 345 350
Asn Gln Arg Asp Ala Asp Leu Leu His Phe Trp Asp Lys Tyr Arg Lys
355 360 365
Ala Pro Glu Gly Thr Pro Arg Lys Ala Glu Ala Gln Lys Gln Phe Phe
370 375 380
Glu Ala Met Ser His Arg Met His Val Asp His Ser Ile Lys Leu Ile
385 390 395 400
Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly Pro Glu Ile Leu Asn Thr
405 410 415
Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Gly Cys Leu Lys
420 425 430
Ser Leu Val Arg Thr Phe Glu Ser His Cys Gly Ala Leu Ser Gln Tyr
435 440 445
Gly Met Lys His Met Arg Ser Leu Ala Asn Ile Cys Asn Thr Gly Ile
450 455 460
Gly Lys Glu Lys Met Ala Glu Ala Ser Ala Gln Ala Cys Glu Asn Ile
465 470 475 480
Pro Ser Gly Pro Trp Ser Ser Leu Asp Lys Gly Phe Ser Ala
485 490
<210> 51
<211> 484
<212> PRT
<213> Capsicum annuum
<400> 51
Met Asn Tyr Gln Val Ala Gly Ile Leu Phe Ile Val Gly Leu Ser Val
1 5 10 15
Ala Ile Ala Val Thr Ala Val Asp Gly Arg Asn Val Leu Lys Leu Pro
20 25 30
Thr Glu Ala Ser Arg Phe Phe Asp His Ala Asp Asp Asp Ser Val Gly
35 40 45
Thr Arg Trp Ala Val Leu Leu Ala Gly Ser Asn Gly Tyr Trp Asn Tyr
50 55 60
Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Leu Leu Arg Lys Gly
65 70 75 80
Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala
85 90 95
Tyr Asn Glu Glu Asn Pro Arg Pro Gly Val Ile Ile Asn Asn Pro Ala
100 105 110
Ala Glu Asp Val Tyr Glu Gly Val Pro Lys Asp Tyr Thr Arg Asp Glu
115 120 125
Val Asn Val His Asn Phe Leu Ala Val Leu Leu Gly Asn Lys Thr Ala
130 135 140
Leu Thr Gly Gly Ser Gly Lys Val Val Asn Ser Gly Pro Asn Asp His
145 150 155 160
Ile Phe Ile Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met
165 170 175
Pro Thr Asn Pro Tyr Leu Tyr Ala Ser Asp Leu Ile Asn Ala Leu Lys
180 185 190
Lys Lys His Ala Ala Gly Ala Tyr Lys Ser Leu Val Leu Tyr Ile Glu
195 200 205
Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Thr Gly Leu
210 215 220
Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Val Glu Ser Ser Trp Gly
225 230 235 240
Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Glu Thr
245 250 255
Cys Leu Gly Asp Leu Tyr Ala Val Ser Trp Met Glu Asp Ser Glu Met
260 265 270
His Asn Leu Arg Thr Glu Asn Leu Arg Gln Gln Tyr His Leu Val Lys
275 280 285
Arg Arg Thr Ala Asn Gly Asn Thr Cys Gly Ser His Val Met Gln Phe
290 295 300
Gly Asp Leu Gln Leu Ser Met Glu Ser Leu Phe Ser Phe Met Gly Thr
305 310 315 320
Asn Pro Ala Asn Asp Asn Tyr Thr Tyr Val Asp Asp Asn Ser Leu Trp
325 330 335
Ala Ser Ser Arg Ala Val Asn Gln Arg Asp Ala Asp Leu Leu His Phe
340 345 350
Trp Asp Lys Phe Arg Lys Ala Pro Glu Gly Ser Ala Arg Lys Val Glu
355 360 365
Ala Gln Lys Gln Phe Thr Glu Ala Met Ser His Arg Met His Leu Asp
370 375 380
Asn Ser Ile Ala Leu Val Gly Lys Leu Leu Phe Gly Ile Gln Lys Gly
385 390 395 400
Pro Glu Val Leu Lys Arg Val Arg Ser Ala Gly Gln Pro Leu Val Asp
405 410 415
Asp Trp Ala Cys Leu Lys Ser Phe Val Arg Thr Phe Glu Thr His Cys
420 425 430
Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Ile Ala Asn
435 440 445
Ile Cys Asn Ala Gly Ile His Thr Glu Gln Met Val Glu Ala Ser Ala
450 455 460
Gln Ala Cys Pro Ser Ile Pro Ala Asn Thr Trp Ser Ser Leu His Arg
465 470 475 480
Gly Phe Ser Ala
<210> 52
<211> 480
<212> PRT
<213> Solanum lycopersicum
<400> 52
Met Val His Val Ala Gly Val Phe Ile Leu Val Gly Ile Ala Val Leu
1 5 10 15
Ala Ala Val Glu Gly Arg Asn Val Leu Lys Leu Pro Ser Glu Ala Ser
20 25 30
Arg Phe Phe Asp Asp Ala Asp Asp Ser Val Gly Thr Arg Trp Ala Val
35 40 45
Leu Leu Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp
50 55 60
Val Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Asp Glu
65 70 75 80
Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala His His Glu Glu Asn
85 90 95
Pro Arg Pro Gly Val Ile Ile Asn Ser Pro Ala Gly Glu Asp Val Tyr
100 105 110
Glu Gly Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Asn Val His Asn
115 120 125
Phe Leu Ala Val Leu Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser
130 135 140
Gly Lys Val Val Asn Ser Gly Pro Asn Asp His Ile Phe Ile Phe Tyr
145 150 155 160
Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Asn Pro Tyr
165 170 175
Leu Tyr Ala Asp Asp Leu Ile Ala Val Leu Lys Lys Lys His Ala Ala
180 185 190
Gly Thr Tyr Lys Ser Leu Val Leu Tyr Ile Glu Ala Cys Glu Ser Gly
195 200 205
Ser Ile Phe Glu Gly Leu Leu Pro Asn Gly Leu Asn Ile Tyr Ala Thr
210 215 220
Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly
225 230 235 240
Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu
245 250 255
Tyr Ala Val Ser Trp Met Glu Asp Ser Glu Met His Asn Leu Arg Thr
260 265 270
Glu Asn Leu Arg Gln Gln Tyr His Leu Val Lys Lys Arg Thr Ala Asn
275 280 285
Gly Asn Thr Ala Tyr Gly Ser His Val Met Gln Phe Gly Asp Leu Gln
290 295 300
Leu Ser Met Glu Ser Leu Phe Arg Phe Met Gly Thr Asn Pro Ala Asn
305 310 315 320
Asp Asn Tyr Thr Tyr Val Asp Asp Asn Ser Leu Leu Ala Ser Ser Lys
325 330 335
Ala Val Asn Gln Arg Asp Ala Asp Leu Leu His Phe Trp Asp Lys Phe
340 345 350
Arg Lys Ala Pro Glu Gly Ser Ala Arg Lys Val Glu Ala Gln Lys Gln
355 360 365
Phe Thr Glu Ala Met Ser His Arg Met His Leu Asp Glu Arg Ile Ala
370 375 380
Leu Val Gly Lys Leu Leu Phe Gly Ile Gln Lys Gly Pro Glu Val Leu
385 390 395 400
Lys His Val Arg Ser Ala Gly Gln Pro Leu Val Asp Asp Trp Ala Cys
405 410 415
Leu Lys Ser Phe Val Arg Thr Phe Glu Ser His Cys Gly Ser Leu Ser
420 425 430
Gln Tyr Gly Met Lys His Met Arg Ser Ile Ala Asn Ile Cys Asn Ala
435 440 445
Gly Ile Gln Met Glu Gln Met Val Glu Ala Ser Ala Gln Ala Cys Pro
450 455 460
Ser Ile Pro Ser Asn Ile Trp Ser Ser Leu His Arg Gly Phe Ser Ala
465 470 475 480
<210> 53
<211> 480
<212> PRT
<213> Solanum tuberosum
<400> 53
Met Ile His Val Ala Gly Val Phe Ile Leu Val Gly Val Ala Val Leu
1 5 10 15
Ala Ala Val Glu Gly Arg Asn Val Leu Lys Leu Pro Ser Glu Ala Ser
20 25 30
Arg Phe Phe Asp Val Ala Asp Asp Ser Val Gly Thr Arg Trp Ala Val
35 40 45
Leu Leu Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp
50 55 60
Val Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Asp Glu
65 70 75 80
Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala His His Glu Glu Asn
85 90 95
Pro Arg Pro Gly Val Ile Ile Asn Ser Pro Ala Gly Glu Asp Val Tyr
100 105 110
Glu Gly Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Asn Val His Asn
115 120 125
Phe Leu Thr Val Leu Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser
130 135 140
Gly Lys Val Val Asn Ser Gly Pro Asn Asp His Ile Phe Ile Phe Tyr
145 150 155 160
Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Asn Pro Tyr
165 170 175
Leu Tyr Ala Asn Asp Leu Ile Ala Val Leu Lys Lys Lys His Ala Ala
180 185 190
Gly Thr Tyr Lys Ser Leu Val Leu Tyr Ile Glu Ala Cys Glu Ser Gly
195 200 205
Ser Ile Phe Glu Gly Leu Leu Pro Lys Gly Leu Asn Ile Tyr Ala Thr
210 215 220
Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly
225 230 235 240
Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu
245 250 255
Tyr Ala Val Ser Trp Met Glu Asp Ser Glu Met His Asn Leu Arg Thr
260 265 270
Glu Asn Leu Arg Gln Gln Tyr His Leu Val Lys Lys Arg Thr Ala Asn
275 280 285
Gly Asn Thr Ala Tyr Gly Ser His Val Met Gln Phe Gly Asp Leu Gln
290 295 300
Leu Ser Met Glu Ser Leu Phe Arg Phe Met Gly Thr Asn Pro Ala Asn
305 310 315 320
Asp Asn Tyr Thr Tyr Val Asp Asp Asn Ser Leu Trp Ala Ser Ser Lys
325 330 335
Ala Val Asn Gln Arg Asp Ala Asp Leu Leu His Phe Trp Asp Lys Phe
340 345 350
Arg Lys Ala Pro Glu Gly Ser Ala Arg Lys Val Glu Ala Gln Lys Gln
355 360 365
Phe Thr Glu Ala Met Ser His Arg Met His Leu Asp Glu Arg Ile Ala
370 375 380
Leu Val Gly Lys Leu Leu Phe Gly Ile Gln Lys Gly Pro Glu Val Leu
385 390 395 400
Lys His Val Arg Ser Ala Gly Gln Pro Leu Val Asp Asp Trp Ala Cys
405 410 415
Leu Lys Ser Phe Val Arg Thr Phe Glu Ser His Cys Gly Ser Leu Ser
420 425 430
Gln Tyr Gly Met Lys His Met Arg Ser Ile Ala Asn Ile Cys Asn Ala
435 440 445
Gly Val Gln Met Glu Gln Met Val Glu Ala Ser Val Gln Ala Cys Pro
450 455 460
Ser Ile Pro Ser Asn Thr Trp Ser Ser Leu His Arg Gly Phe Ser Ala
465 470 475 480
<210> 54
<211> 480
<212> PRT
<213> Solanum lycopersicum
<400> 54
Met Val His Val Ala Gly Val Phe Ile Leu Val Gly Ile Ala Val Leu
1 5 10 15
Ala Ala Val Glu Gly Arg Asn Val Leu Lys Leu Pro Ser Glu Ala Ser
20 25 30
Arg Phe Phe Asp Asp Ala Asp Asp Ser Val Gly Thr Arg Trp Ala Val
35 40 45
Leu Leu Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp
50 55 60
Val Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Asp Glu
65 70 75 80
Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala His His Glu Glu Asn
85 90 95
Pro Arg Pro Gly Val Ile Ile Asn Ser Pro Ala Gly Glu Asp Val Tyr
100 105 110
Glu Gly Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Asn Val His Asn
115 120 125
Phe Leu Ala Val Leu Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser
130 135 140
Gly Lys Val Val Asn Ser Gly Pro Asn Asp His Ile Phe Ile Phe Tyr
145 150 155 160
Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Asn Pro Tyr
165 170 175
Leu Tyr Ala Asp Asp Leu Ile Ala Val Leu Lys Lys Lys His Ala Pro
180 185 190
Gly Thr Tyr Lys Ser Leu Val Leu Tyr Ile Glu Ala Cys Glu Ser Gly
195 200 205
Ser Ile Phe Glu Gly Leu Leu Pro Asn Gly Leu Asn Ile Tyr Ala Thr
210 215 220
Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly
225 230 235 240
Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu
245 250 255
Tyr Ala Val Ser Trp Met Glu Asp Ser Glu Met His Asn Leu Arg Thr
260 265 270
Glu Asn Leu Arg Gln Gln Tyr His Leu Val Lys Lys Arg Thr Ala Asn
275 280 285
Gly Asn Thr Ala Tyr Gly Ser His Val Met Gln Phe Gly Asp Leu Gln
290 295 300
Leu Ser Met Glu Ser Leu Phe Arg Phe Met Gly Thr Asn Pro Ala Asn
305 310 315 320
Asp Asn Tyr Thr Tyr Val Asp Asp Asn Ser Leu Leu Ala Ser Ser Lys
325 330 335
Ala Val Asn Gln Arg Asp Ala Asp Leu Leu His Phe Trp Asp Lys Phe
340 345 350
Arg Lys Ala Pro Glu Gly Ser Ala Arg Lys Val Glu Ala Gln Lys Gln
355 360 365
Phe Thr Glu Ala Met Ser His Arg Met His Leu Asp Glu Arg Ile Ala
370 375 380
Leu Val Gly Lys Leu Leu Phe Gly Ile Gln Lys Gly Pro Glu Val Leu
385 390 395 400
Lys His Val Arg Ser Ala Gly Gln Pro Leu Val Asp Asp Trp Ala Cys
405 410 415
Leu Lys Ser Phe Val Arg Thr Phe Glu Ser His Cys Gly Ser Leu Ser
420 425 430
Gln Tyr Gly Met Lys His Met Arg Ser Ile Ala Asn Ile Cys Asn Ala
435 440 445
Gly Ile Gln Met Glu Gln Met Val Glu Ala Ser Ala Gln Ala Cys Pro
450 455 460
Ser Ile Pro Ser Asn Ile Trp Ser Ser Leu His Arg Gly Phe Ser Ala
465 470 475 480
<210> 55
<211> 490
<212> PRT
<213> Eutrema salsugineum
<400> 55
Met Ser Arg Val Ser Val Thr Val Thr Val Ala Val Ser Phe Leu Ala
1 5 10 15
Leu Phe Ile Ser Leu Val Thr Val Ser Cys Asp Val Ile Lys Leu Pro
20 25 30
Ser Gln Ala Ser Lys Phe Phe Arg Thr Thr Lys His Asn Asp Asp Gly
35 40 45
Asp Ser Ser Ala Gly Thr Lys Trp Ala Val Leu Val Ala Gly Ser Ser
50 55 60
Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln
65 70 75 80
Leu Leu Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile Ile Val Phe Met
85 90 95
Tyr Asp Asp Ile Ala Lys Asn Lys Glu Asn Pro Arg Pro Gly Ile Ile
100 105 110
Ile Asn Ser Pro Asn Gly Asn Asp Val Tyr Asn Gly Val Pro Lys Asp
115 120 125
Tyr Thr Gly Asp Asp Val Asn Val Asp Asn Leu Phe Ala Val Ile Leu
130 135 140
Ala Asn Lys Thr Ala Leu Lys Gly Gly Ser Gly Lys Val Val Asp Ser
145 150 155 160
Gly Pro Asp Asp His Ile Phe Ile Tyr Tyr Ser Asp His Gly Gly Pro
165 170 175
Gly Val Leu Gly Met Pro Thr Ser Pro His Leu Tyr Ala Asn Asp Leu
180 185 190
Ile Asp Ile Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu
195 200 205
Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu
210 215 220
Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu
225 230 235 240
Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Asp Asp Ser Ser Pro Pro
245 250 255
Lys Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val Ser Trp Met
260 265 270
Glu Asp Ser Asp Gln His Asn Leu Gln Thr Glu Ser Leu His Gln Gln
275 280 285
Tyr Glu Leu Val Lys Lys Arg Thr Ala Gly Ser Asn Thr Ser Tyr Gly
290 295 300
Ser His Val Met Glu Phe Gly Asp Ile Gly Leu Ser Lys Glu Met Leu
305 310 315 320
Val Leu Tyr Met Gly Thr Asn Pro Asp Asn Glu Asn Tyr Thr Phe Val
325 330 335
Asp Lys Asn Ser Leu Arg Pro Pro Ser Arg Val Thr Asn Gln Arg Asp
340 345 350
Ala Asp Leu Val His Phe Trp Asp Lys Tyr Gln Lys Ala Pro Glu Gly
355 360 365
Ser Ala Arg Lys Ala Glu Ala Gln Lys Gln Val Leu Glu Ala Met Ser
370 375 380
His Arg Leu His Ile Asp Asn Ser Phe Leu Met Ile Gly Lys Leu Leu
385 390 395 400
Phe Gly Ile Ser Glu Gly Pro Leu Val Leu Asn Lys Val Arg Pro Ser
405 410 415
Gly Lys Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Thr Leu Val Arg
420 425 430
Ala Tyr Glu Arg His Cys Gly Ser Leu Ser Gln Tyr Gly Ile Lys His
435 440 445
Met Arg Ser Ile Ala Asn Ile Cys Asn Ala Gly Ile Gln Val Glu Gln
450 455 460
Met Glu Glu Ala Ala Met Gln Ala Cys Pro Thr Ile Pro Ala Gly Pro
465 470 475 480
Trp Ser Ser Leu His Arg Gly Phe Ser Ala
485 490
<210> 56
<211> 492
<212> PRT
<213> Ipomoea batatas
<400> 56
Met Ile Arg Ser Val Val Ala Ser Leu Leu Leu Leu Thr Val Ser Ile
1 5 10 15
Val Ala Val Ala Asp Gly Arg Gly Phe Leu Lys Leu Pro Ser Glu Ala
20 25 30
Arg Arg Phe Phe Arg Pro Ala Glu Glu Glu Asn Arg Glu Ala Asp Gly
35 40 45
Asp Asp Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Asn
50 55 60
Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln
65 70 75 80
Ile Leu Lys Ala Gly Gly Leu Lys Asp Glu Asn Ile Val Val Phe Met
85 90 95
Tyr Asp Asp Ile Ala Tyr Asn Glu Glu Asn Pro Arg Lys Gly Ile Ile
100 105 110
Ile Asn Ser Pro His Gly Glu Asp Val Tyr His Gly Val Pro Lys Asp
115 120 125
Tyr Thr Gly Asp Asp Val Thr Ala Asn Asn Leu Leu Ala Val Ile Leu
130 135 140
Gly Asp Lys Ser Ala Val Lys Gly Gly Ser Gly Lys Val Val Asp Ser
145 150 155 160
Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His Gly Gly Pro
165 170 175
Gly Val Leu Gly Met Pro Thr Ser Pro Tyr Leu Tyr Ala Asp Glu Leu
180 185 190
Asn Ala Ala Leu Lys Lys Lys His Ala Ala Gly Ala Tyr Lys Ser Leu
195 200 205
Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Ile
210 215 220
Leu Pro Lys Asp Ile Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Ile
225 230 235 240
Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro
245 250 255
Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ala Trp Met
260 265 270
Glu Asp Ser Asp Ile His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln
275 280 285
Tyr Asn Leu Val Lys Asp Arg Thr Leu Asn Gly Asn Thr Ala Tyr Gly
290 295 300
Ser His Val Met Gln Tyr Gly Asp Leu Glu Leu Asn Ala Asp Ser Leu
305 310 315 320
Phe Met Tyr Met Gly Thr Asn Pro Ala Asn Glu Asn Phe Thr Phe Val
325 330 335
Asp Glu Lys Ser Leu Lys Leu Ser Ala Pro Arg Arg Ala Val Asn Gln
340 345 350
Arg Asp Ala Asp Leu Leu His Phe Trp Asp Lys Phe Arg Asn Ala Pro
355 360 365
Glu Gly Ser Ala Arg Lys Ser Glu Ala Gln Lys Gln Phe Thr Glu Ala
370 375 380
Ile Thr His Arg Thr His Leu Asp Asn Ser Ile Ala Leu Val Gly Lys
385 390 395 400
Leu Leu Phe Gly Met Glu Lys Gly Pro Glu Val Leu Ser Ser Val Arg
405 410 415
Ala Thr Gly Leu Pro Leu Val Asp Asp Trp Ser Cys Leu Lys Ser Tyr
420 425 430
Val Arg Ala Phe Glu Thr His Cys Gly Ser Leu Ser Gln Tyr Gly Met
435 440 445
Lys His Met Arg Ser Ile Ala Asn Ile Cys Asn Ala Gly Ile Ser Glu
450 455 460
Glu Arg Met Ala Glu Ala Ser Ala Gln Ala Cys Pro Thr Phe Pro Ser
465 470 475 480
Tyr Ser Trp Ser Ser Leu Arg Gly Gly Phe Ser Ala
485 490
<210> 57
<211> 490
<212> PRT
<213> Nicotiana tabacum
<400> 57
Met Ile Arg His Ile Ala Gly Thr Leu Phe Ile Ile Gly Leu Ala Leu
1 5 10 15
Asn Val Ala Val Ser Glu Ser Arg Asn Val Leu Lys Leu Pro Ser Glu
20 25 30
Val Ser Arg Phe Phe Gly Ala Asp Lys Ser Asn Val Gly Asp Asp His
35 40 45
Asp Asp Asp Ser Val Gly Thr Arg Trp Ala Ile Leu Leu Ala Gly Ser
50 55 60
Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr
65 70 75 80
Gln Leu Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Val Val Phe
85 90 95
Met Tyr Asp Asp Ile Ala Asn Asn Glu Glu Asn Pro Arg Pro Gly Val
100 105 110
Ile Ile Asn Ser Pro His Gly Glu Asp Val Tyr Lys Gly Val Pro Lys
115 120 125
Asp Tyr Thr Gly Asp Asp Val Thr Val Asn Asn Phe Phe Ala Ala Leu
130 135 140
Leu Gly Asn Lys Thr Ala Leu Ser Gly Gly Ser Gly Lys Val Val Asn
145 150 155 160
Ser Gly Pro Asn Asp His Ile Leu Ile Phe Tyr Ser Asp His Gly Gly
165 170 175
Pro Gly Val Leu Gly Met Pro Thr Asp Pro Tyr Leu Tyr Ala Asn Asp
180 185 190
Leu Ile Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser
195 200 205
Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly
210 215 220
Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala
225 230 235 240
Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro
245 250 255
Pro Ile Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ser Trp
260 265 270
Met Glu Asp Ser Glu Leu His Asn Leu Arg Thr Glu Ser Leu Lys Gln
275 280 285
Gln Tyr His Leu Val Arg Glu Arg Thr Ala Thr Gly Asn Pro Val Tyr
290 295 300
Gly Ser His Val Met Gln Tyr Gly Asp Leu His Leu Ser Lys Asp Ala
305 310 315 320
Leu Tyr Leu Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Tyr Thr Phe
325 330 335
Met Asp Asp Asn Ser Leu Arg Val Ser Lys Ala Val Asn Gln Arg Asp
340 345 350
Ala Asp Leu Leu His Phe Trp Tyr Lys Phe Arg Lys Ala Pro Glu Gly
355 360 365
Ser Val Arg Lys Ile Glu Ala Gln Lys Gln Leu Asn Glu Ala Ile Ser
370 375 380
His Arg Val His Leu Asp Asn Ser Ile Ala Leu Val Gly Lys Leu Leu
385 390 395 400
Phe Gly Ile Lys Lys Gly Pro Glu Val Leu Ser Ser Val Arg Pro Ala
405 410 415
Gly Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Ser Phe Val Arg
420 425 430
Thr Phe Glu Thr His Cys Gly Ser Leu Ser Gln Tyr Gly Met Lys His
435 440 445
Met Arg Ser Ile Ala Asn Ile Cys Asn Val Gly Ile Lys Met Ala Gln
450 455 460
Met Val Glu Ala Ser Ala Gln Ala Cys Pro Ser Phe Ala Ser Asn Thr
465 470 475 480
Trp Ser Ser Leu Gln Arg Gly Phe Ser Ala
485 490
<210> 58
<211> 494
<212> PRT
<213> Arabidopsis lyrata subsp. lyrata
<400> 58
Met Ala Thr Thr Met Thr Arg Val Pro Val Gly Ala Phe Leu Leu Val
1 5 10 15
Leu Leu Val Ser Leu Val Ala Val Ser Thr Ala Arg Ser Gly Pro Asp
20 25 30
Asp Val Ile Lys Leu Pro Ser Gln Ala Ser Arg Phe Phe Arg Pro Ala
35 40 45
Gln Asp Asp Asp Asp Ser Asn Ala Gly Thr Arg Trp Ala Val Leu Val
50 55 60
Ala Gly Ser Ser Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys
65 70 75 80
His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile
85 90 95
Val Val Phe Met Tyr Asp Asp Ile Ala Asn Asn Tyr Glu Asn Pro Arg
100 105 110
Pro Gly Thr Leu Ile Asn Ser Pro His Gly Lys Asp Val Tyr Gln Gly
115 120 125
Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Asn Val Asp Asn Leu Phe
130 135 140
Ala Val Ile Leu Gly Asp Lys Thr Ala Val Lys Gly Gly Ser Gly Lys
145 150 155 160
Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Phe Tyr Ser Asp
165 170 175
His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Pro Tyr Leu Tyr
180 185 190
Ala Asn Asp Leu Asn Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr
195 200 205
Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile
210 215 220
Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala
225 230 235 240
Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Glu
245 250 255
Pro Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser
260 265 270
Val Ala Trp Met Glu Asp Ser Gly Met His Asn Leu Gln Thr Glu Thr
275 280 285
Leu His Gln Gln Tyr Glu Leu Val Lys Arg Arg Thr Ala Pro Val Gly
290 295 300
Tyr Ser Tyr Gly Ser His Val Met Gln Tyr Gly Asp Val Gly Leu Ser
305 310 315 320
Lys Asp Asn Leu Asp Leu Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn
325 330 335
Phe Thr Phe Ala Asp Ala Asn Ser Leu Lys Pro Pro Ser Arg Val Thr
340 345 350
Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp Glu Lys Tyr Arg Lys
355 360 365
Ala Pro Glu Gly Ser Ala Arg Lys Thr Glu Ala Gln Lys Gln Val Leu
370 375 380
Glu Ala Met Ser His Arg Leu His Val Asp Asn Ser Val Ile Leu Val
385 390 395 400
Gly Lys Ile Leu Phe Gly Ile Ser Glu Gly Pro Glu Val Leu Asn Lys
405 410 415
Val Arg Ser Ala Gly Gln Pro Leu Val Asp Asp Trp Asn Cys Leu Lys
420 425 430
Asn Leu Val Arg Ala Phe Glu Arg His Cys Gly Ser Leu Ser Gln Tyr
435 440 445
Gly Ile Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile
450 455 460
Arg Thr Glu Gln Met Glu Glu Ala Ala Ser Gln Ala Cys Thr Ser Ile
465 470 475 480
Pro Pro Gly Pro Trp Ser Ser Leu His Arg Gly Phe Ser Ala
485 490
<210> 59
<211> 478
<212> PRT
<213> Thellungiella halophila
<400> 59
Met Thr Ser Val Ala Val Pro Leu Leu Val Leu Leu Leu Ser Leu Ile
1 5 10 15
Ala Val Ser Ala Ala Arg Gln Gly Pro Asp Asp Ile Ile Lys Leu Pro
20 25 30
Ser Gln Ala Ser Met Phe Phe Arg Pro Ala Asp Asp Asp Asn Asp Ser
35 40 45
Ser Ala Gly Thr Arg Trp Ala Val Leu Val Ala Gly Ser Asn Gly Tyr
50 55 60
Trp Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln Leu Leu
65 70 75 80
Arg Lys Gly Gly Val Lys Glu Asp Asn Ile Val Val Phe Met Tyr Asp
85 90 95
Asp Ile Ala Asn Asn Glu Glu Asn Pro Arg Arg Gly Ile Ile Ile Asn
100 105 110
Ser Pro His Gly Lys Asp Val Tyr Gln Gly Val Pro Lys Asp Tyr Thr
115 120 125
Gly Asp Asp Val Thr Val Asp Asn Leu Phe Ala Val Ile Leu Gly Asn
130 135 140
Lys Thr Ala Thr Lys Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro
145 150 155 160
Asn Asp His Ile Phe Ile Phe Tyr Ser Asp His Gly Gly Pro Gly Val
165 170 175
Leu Gly Met Pro Thr Ser Pro Tyr Leu Tyr Ala Asn Asp Leu Asn Asp
180 185 190
Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe
195 200 205
Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Glu
210 215 220
Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Val Glu Ser
225 230 235 240
Ser Trp Gly Thr Tyr Cys Pro Gly Glu Asp Pro Ser Leu Pro Pro Glu
245 250 255
Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val Ser Trp Met Glu Asp
260 265 270
Ser Gly Met His Asn Leu Gln Thr Glu Thr Leu Arg Gln Gln Tyr Glu
275 280 285
Leu Val Lys Arg Arg Thr Ala Gly Val Gly Ser Ala Tyr Gly Ser His
290 295 300
Val Met Gln Tyr Gly Asp Val Gly Leu Ser Lys Asp Lys Leu Asp Leu
305 310 315 320
Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Phe Thr Phe Val Asp Glu
325 330 335
Asn Ser Leu Thr Pro Pro Ser Arg Val Thr Asn Gln Arg Asp Ala Asp
340 345 350
Leu Val His Phe Trp Asp Lys Tyr Arg Lys Ala Pro Glu Gly Ser Thr
355 360 365
Arg Lys Thr Glu Ala Gln Lys Gln Val Leu Glu Ala Met Ser His Arg
370 375 380
Leu His Val Asp Asn Ser Val Lys Leu Val Gly Lys Leu Leu Phe Gly
385 390 395 400
Ile Ser Glu Gly Pro Glu Val Leu Asn Lys Val Arg Ser Ala Gly Gln
405 410 415
Pro Leu Val Asp Asp Trp Asn Cys Leu Lys Asn Leu Val Arg Ala Phe
420 425 430
Glu Arg His Cys Gly Ser Leu Ser Gln Tyr Gly Ile Lys His Met Arg
435 440 445
Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile Gln Met Glu Gln Met Glu
450 455 460
Glu Ala Ser Ser Gln Ala Cys Thr Thr Ile Pro Pro Gly Pro
465 470 475
<210> 60
<211> 486
<212> PRT
<213> Beta vulgaris
<400> 60
Met Met Ile Arg Tyr Thr Ser Gly Val Leu Ile Val Leu Cys Val Leu
1 5 10 15
Met Ser Ser Val Val Asp Ser Arg Leu Met Val Asp Asn Leu Ile Arg
20 25 30
Trp Pro Ser Asp His Pro Ser Ile Phe Glu Ser Asp Asp Asp Ser Val
35 40 45
Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Ser Gly Tyr Trp Asn
50 55 60
Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Val Leu Lys Lys
65 70 75 80
Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile
85 90 95
Ala Tyr Asp Glu Glu Asn Pro Arg Pro Gly Val Leu Ile Asn Ser Pro
100 105 110
Tyr Gly His Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Glu
115 120 125
Asp Val Thr Val Asn Asn Phe Phe Ala Ala Ile Leu Gly Asn Lys Asp
130 135 140
Ala Ile Thr Gly Gly Ser Gly Lys Val Val Asn Ser Gly Pro Asn Asp
145 150 155 160
His Ile Phe Ile Phe Tyr Ser Asp His Gly Gly Ala Gly Val Leu Gly
165 170 175
Met Pro Thr Tyr Pro Tyr Leu Tyr Ala Asp Glu Leu Ile Glu Thr Leu
180 185 190
Lys Glu Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Val Tyr Ile
195 200 205
Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Ile Leu Pro Glu Gly
210 215 220
Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Val Glu Ser Ser Trp
225 230 235 240
Gly Thr Tyr Cys Pro Gly Gln Asp Pro Asn Val Pro Pro Glu Tyr Asp
245 250 255
Thr Cys Leu Gly Asp Leu Tyr Ser Val Ser Trp Ile Glu Asp Ser Glu
260 265 270
Arg His Asn Leu His Thr Glu Ser Leu Lys Gln Gln Tyr Glu Val Val
275 280 285
Lys Thr Lys Thr Ala Glu Lys Pro Phe Tyr Gly Ser His Val Met Gln
290 295 300
Tyr Gly Asp Lys Glu Leu Thr Gln Asp Met Leu Tyr Leu Tyr Met Gly
305 310 315 320
Thr Asn Pro Asn Asn Glu Asn Tyr Thr Tyr Val Asp Asp Asn Ser Leu
325 330 335
His Pro Thr Ser Ser Asn Ala Val Asn Gln Arg Asp Ala Asp Leu Ile
340 345 350
His Phe Trp Asn Lys Phe Arg Lys Ala Ser Glu Gly Ser Gln Arg Lys
355 360 365
Ile Asn Ala Gln Lys Gln Phe Met Glu Val Met Ser His Arg Val His
370 375 380
Leu Asp Asp Ser Ile Lys Leu Ile Gly Lys Leu Leu Phe Gly Ile Glu
385 390 395 400
Lys Gly Leu Gly Val Leu Gln Thr Val Arg Pro Thr Gly Gln Pro Leu
405 410 415
Val Asp Asp Trp Asn Cys Leu Lys Thr Leu Val Arg Thr Phe Glu Lys
420 425 430
His Cys Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser Ile
435 440 445
Ala Asn Ile Cys Asn Ala Gly Ile Thr Thr Asn Gln Met Ala Glu Ala
450 455 460
Ser Ala Gln Ala Cys Pro Ser Phe Pro Ser Gly Pro Trp Ser Ser Leu
465 470 475 480
His Arg Gly Phe Ser Ala
485
<210> 61
<211> 494
<212> PRT
<213> Arabidopsis thaliana
<400> 61
Met Ala Thr Thr Met Thr Arg Val Ser Val Gly Val Val Leu Phe Val
1 5 10 15
Leu Leu Val Ser Leu Val Ala Val Ser Ala Ala Arg Ser Gly Pro Asp
20 25 30
Asp Val Ile Lys Leu Pro Ser Gln Ala Ser Arg Phe Phe Arg Pro Ala
35 40 45
Glu Asn Asp Asp Asp Ser Asn Ser Gly Thr Arg Trp Ala Val Leu Val
50 55 60
Ala Gly Ser Ser Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys
65 70 75 80
His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile
85 90 95
Val Val Phe Met Tyr Asp Asp Ile Ala Asn Asn Tyr Glu Asn Pro Arg
100 105 110
Pro Gly Thr Ile Ile Asn Ser Pro His Gly Lys Asp Val Tyr Gln Gly
115 120 125
Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Asn Val Asp Asn Leu Phe
130 135 140
Ala Val Ile Leu Gly Asp Lys Thr Ala Val Lys Gly Gly Ser Gly Lys
145 150 155 160
Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Phe Tyr Ser Asp
165 170 175
His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Pro Tyr Leu Tyr
180 185 190
Ala Asn Asp Leu Asn Asp Val Leu Lys Lys Lys His Ala Leu Gly Thr
195 200 205
Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile
210 215 220
Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala
225 230 235 240
Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Glu
245 250 255
Pro Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser
260 265 270
Val Ala Trp Met Glu Asp Ser Gly Met His Asn Leu Gln Thr Glu Thr
275 280 285
Leu His Gln Gln Tyr Glu Leu Val Lys Arg Arg Thr Ala Pro Val Gly
290 295 300
Tyr Ser Tyr Gly Ser His Val Met Gln Tyr Gly Asp Val Gly Ile Ser
305 310 315 320
Lys Asp Asn Leu Asp Leu Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn
325 330 335
Phe Thr Phe Ala Asp Ala Asn Ser Leu Lys Pro Pro Ser Arg Val Thr
340 345 350
Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp Glu Lys Tyr Arg Lys
355 360 365
Ala Pro Glu Gly Ser Ala Arg Lys Thr Glu Ala Gln Lys Gln Val Leu
370 375 380
Glu Ala Met Ser His Arg Leu His Ile Asp Asn Ser Val Ile Leu Val
385 390 395 400
Gly Lys Ile Leu Phe Gly Ile Ser Arg Gly Pro Glu Val Leu Asn Lys
405 410 415
Val Arg Ser Ala Gly Gln Pro Leu Val Asp Asp Trp Asn Cys Leu Lys
420 425 430
Asn Gln Val Arg Ala Phe Glu Arg His Cys Gly Ser Leu Ser Gln Tyr
435 440 445
Gly Ile Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile
450 455 460
Gln Met Glu Gln Met Glu Glu Ala Ala Ser Gln Ala Cys Thr Thr Leu
465 470 475 480
Pro Thr Gly Pro Trp Ser Ser Leu Asn Arg Gly Phe Ser Ala
485 490
<210> 62
<211> 490
<212> PRT
<213> Arabidopsis thaliana
<400> 62
Met Thr Arg Val Ser Val Gly Val Val Leu Phe Val Leu Leu Val Ser
1 5 10 15
Leu Val Ala Val Ser Ala Ala Arg Ser Gly Pro Asp Asp Val Ile Lys
20 25 30
Leu Pro Ser Gln Ala Ser Arg Phe Phe Arg Pro Ala Glu Asn Asp Asp
35 40 45
Asp Ser Asn Ser Gly Thr Arg Trp Ala Val Leu Val Ala Gly Ser Ser
50 55 60
Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln
65 70 75 80
Leu Leu Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile Val Val Phe Met
85 90 95
Tyr Asp Asp Ile Ala Asn Asn Tyr Glu Asn Pro Arg Pro Gly Thr Ile
100 105 110
Ile Asn Ser Pro His Gly Lys Asp Val Tyr Gln Gly Val Pro Lys Asp
115 120 125
Tyr Thr Gly Asp Asp Val Asn Val Asp Asn Leu Phe Ala Val Ile Leu
130 135 140
Gly Asp Lys Thr Ala Val Lys Gly Gly Ser Gly Lys Val Val Asp Ser
145 150 155 160
Gly Pro Asn Asp His Ile Phe Ile Phe Tyr Ser Asp His Gly Gly Pro
165 170 175
Gly Val Leu Gly Met Pro Thr Ser Pro Tyr Leu Tyr Ala Asn Asp Leu
180 185 190
Asn Asp Val Leu Lys Lys Lys His Ala Leu Gly Thr Tyr Lys Ser Leu
195 200 205
Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu
210 215 220
Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu
225 230 235 240
Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Glu Pro Ser Pro Pro
245 250 255
Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met
260 265 270
Glu Asp Ser Gly Met His Asn Leu Gln Thr Glu Thr Leu His Gln Gln
275 280 285
Tyr Glu Leu Val Lys Arg Arg Thr Ala Pro Val Gly Tyr Ser Tyr Gly
290 295 300
Ser His Val Met Gln Tyr Gly Asp Val Gly Ile Ser Lys Asp Asn Leu
305 310 315 320
Asp Leu Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Phe Thr Phe Ala
325 330 335
Asp Ala Asn Ser Leu Lys Pro Pro Ser Arg Val Thr Asn Gln Arg Asp
340 345 350
Ala Asp Leu Val His Phe Trp Glu Lys Tyr Arg Lys Ala Pro Glu Gly
355 360 365
Ser Ala Arg Lys Thr Glu Ala Gln Lys Gln Val Leu Glu Ala Met Ser
370 375 380
His Arg Leu His Ile Asp Asn Ser Val Ile Leu Val Gly Lys Ile Leu
385 390 395 400
Phe Gly Ile Ser Arg Gly Pro Glu Val Leu Asn Lys Val Arg Ser Ala
405 410 415
Gly Gln Pro Leu Val Asp Asp Trp Asn Cys Leu Lys Asn Gln Val Arg
420 425 430
Ala Phe Glu Arg His Cys Gly Ser Leu Ser Gln Tyr Gly Ile Lys His
435 440 445
Met Arg Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile Gln Met Glu Gln
450 455 460
Met Glu Glu Ala Ala Ser Gln Ala Cys Thr Thr Leu Pro Thr Gly Pro
465 470 475 480
Trp Ser Ser Leu Asn Arg Gly Phe Ser Ala
485 490
<210> 63
<211> 484
<212> PRT
<213> Nicotiana tabacum
<400> 63
Met Ile Val Arg Tyr Val Val Ser Ala Ile Leu Ile Ile Gly Leu Ser
1 5 10 15
Val Val Ala Ala Val Asp Gly Arg Asp Val Leu Lys Leu Pro Ser Glu
20 25 30
Ala Ser Thr Phe Phe Ser Gly Asn Tyr Asp Asp Asp Ser Ile Gly Thr
35 40 45
Lys Trp Ala Val Leu Val Ala Gly Ser Arg Gly Tyr Trp Asn Tyr Arg
50 55 60
His Gln Ala Asp Val Cys His Ala Tyr Gln Leu Leu Lys Lys Gly Gly
65 70 75 80
Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala His
85 90 95
Asn Phe Glu Asn Pro Arg Pro Gly Val Ile Ile Asn Ser Pro Asn Gly
100 105 110
Asp Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Thr Gly His His Val
115 120 125
Thr Ala Asn Asn Phe Leu Ala Val Ile Leu Gly Asn Lys Ala Ala Leu
130 135 140
Ser Gly Gly Ser Gly Lys Val Val Glu Ser Gly Pro Asn Asp His Ile
145 150 155 160
Phe Ile Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro
165 170 175
Ser Gly Pro Tyr Leu Tyr Ala Asp Asp Leu Ile Asp Val Leu Lys Arg
180 185 190
Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr Ile Glu Ala
195 200 205
Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn
210 215 220
Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Asp Ser Trp Gly Thr
225 230 235 240
Tyr Cys Pro Gly Asp Tyr Pro Gly Pro Pro Pro Glu Tyr Gln Thr Cys
245 250 255
Leu Gly Asp Leu Tyr Ala Val Ser Trp Met Glu Asp Ser Glu Lys His
260 265 270
Asn Leu Arg Arg Glu Thr Leu Gly Met Gln Tyr Glu Leu Val Lys Arg
275 280 285
Arg Thr Ala Asn Ser Phe Pro Tyr Ala Ser Ser His Val Met Gln Tyr
290 295 300
Gly Asp Leu Lys Leu Met Asp Asp Pro Leu Ser Leu Tyr Met Gly Thr
305 310 315 320
Asn Pro Ala Asn Asp Asn Tyr Thr Phe Leu Asp Glu Asn Ser Ser Leu
325 330 335
Leu Ser Ala Lys Pro Val Asn Gln Arg Asp Ala Asp Leu Leu His Phe
340 345 350
Trp Asp Lys Phe Leu Lys Ala Pro Gln Gly Ser Val Arg Lys Val Glu
355 360 365
Ala Gln Lys Gln Leu Ser Glu Ala Met Ser His Arg Met His Ile Asp
370 375 380
Asp Ser Ile Ala Leu Val Gly Arg Leu Leu Phe Gly Ile Glu Lys Gly
385 390 395 400
Pro Asp Val Leu Ile Arg Val Arg Pro Thr Gly Glu Pro Leu Val Asp
405 410 415
Asp Trp Asn Cys Leu Lys Ser Phe Val Arg Thr Phe Glu Thr Arg Cys
420 425 430
Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ala Val Ala Asn
435 440 445
Ile Cys Asn Ser Cys Ile Thr Met Glu Gln Ile Ala Lys Ala Ser Ala
450 455 460
Gln Ala Cys Val Ser Ile Pro Ser Asn Ser Trp Ser Ser Leu Asp Glu
465 470 475 480
Gly Phe Ser Ala
<210> 64
<211> 478
<212> PRT
<213> Arabidopsis thaliana
<400> 64
Met Thr Thr Val Val Ser Phe Leu Ala Leu Phe Leu Phe Leu Val Ala
1 5 10 15
Ala Val Ser Gly Asp Val Ile Lys Leu Pro Ser Leu Ala Ser Lys Phe
20 25 30
Phe Arg Pro Thr Glu Asn Asp Asp Asp Ser Thr Lys Trp Ala Val Leu
35 40 45
Val Ala Gly Ser Ser Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Val
50 55 60
Cys His Ala Tyr Gln Leu Leu Lys Lys Gly Gly Val Lys Glu Glu Asn
65 70 75 80
Ile Val Val Phe Met Tyr Asp Asp Ile Ala Lys Asn Glu Glu Asn Pro
85 90 95
Arg Pro Gly Val Ile Ile Asn Ser Pro Asn Gly Glu Asp Val Tyr Asn
100 105 110
Gly Val Pro Lys Asp Tyr Thr Gly Asp Glu Val Asn Val Asp Asn Leu
115 120 125
Leu Ala Val Ile Leu Gly Asn Lys Thr Ala Leu Lys Gly Gly Ser Gly
130 135 140
Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser
145 150 155 160
Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Pro Asn Leu
165 170 175
Tyr Ala Asn Asp Leu Asn Asp Val Leu Lys Lys Lys Tyr Ala Ser Gly
180 185 190
Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser
195 200 205
Ile Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr
210 215 220
Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu
225 230 235 240
Asp Pro Ser Pro Pro Ser Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr
245 250 255
Ser Val Ala Trp Ile Glu Asp Ser Glu Lys His Asn Leu Gln Thr Glu
260 265 270
Thr Leu His Glu Gln Tyr Glu Leu Val Lys Lys Arg Thr Ala Gly Ser
275 280 285
Gly Lys Ser Tyr Gly Ser His Val Met Glu Phe Gly Asp Ile Gly Leu
290 295 300
Ser Lys Glu Lys Leu Val Leu Phe Met Gly Thr Asn Pro Ala Asp Glu
305 310 315 320
Asn Phe Thr Phe Val Asn Glu Asn Ser Ile Arg Pro Pro Ser Arg Val
325 330 335
Thr Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp His Lys Tyr Gln
340 345 350
Lys Ala Pro Glu Gly Ser Ala Arg Lys Val Glu Ala Gln Lys Gln Val
355 360 365
Leu Glu Ala Met Ser His Arg Leu His Val Asp Asn Ser Ile Leu Leu
370 375 380
Ile Gly Ile Leu Leu Phe Gly Leu Glu Gly His Ala Val Leu Asn Lys
385 390 395 400
Val Arg Pro Ser Gly Glu Pro Leu Val Asp Asp Trp Asp Cys Leu Lys
405 410 415
Ser Leu Val Arg Ala Phe Glu Arg His Cys Gly Ser Leu Ser Gln Tyr
420 425 430
Gly Ile Lys His Met Arg Ser Ile Ala Asn Met Cys Asn Ala Gly Ile
435 440 445
Gln Met Arg Gln Met Glu Glu Ala Ala Met Gln Ala Cys Pro Thr Ile
450 455 460
Pro Thr Ser Pro Trp Ser Ser Leu Asp Arg Gly Phe Ser Ala
465 470 475
<210> 65
<211> 478
<212> PRT
<213> Genlisea aurea
<400> 65
Ile Val Leu Leu Leu Leu Ser Val Ser Ile Leu Thr Ser Asp Cys Arg
1 5 10 15
Asn Gln Phe Ile Lys Leu Pro Ser Glu Val Phe Ser Tyr Gly Gly Glu
20 25 30
Ser Glu Asp Asp Ser Val Gly Thr Arg Trp Ala Val Leu Val Ala Gly
35 40 45
Ser Ser Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His Ala
50 55 60
Tyr Gln Ile Leu Lys Arg Gly Gly Leu Lys Asp Glu Asn Ile Ile Val
65 70 75 80
Phe Met Tyr Asp Asp Ile Ala Gln Asn Leu Glu Asn Pro Arg Pro Gly
85 90 95
Ile Val Ile Asn Asn Pro His Gly Glu Asp Val Tyr His Gly Val Pro
100 105 110
Lys Asp Tyr Val Gly Arg Gln Val Thr Ala His Asn Phe Tyr Ser Val
115 120 125
Leu Leu Gly Asp Lys Ala Gly Leu Thr Gly Gly Ser Gly Lys Val Ile
130 135 140
Glu Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His Gly
145 150 155 160
Gly Pro Gly Val Leu Gly Met Pro Ser Gly Pro Tyr Ile Tyr Ala Asp
165 170 175
Asp Leu Asn Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys
180 185 190
Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu
195 200 205
Gly Ile Leu Pro Asp Asn Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn
210 215 220
Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Asp Ile Ile Ala
225 230 235 240
Pro Pro Leu Glu Tyr Asp Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ala
245 250 255
Trp Met Glu Asp Ser Asp Ile His Asn Leu Arg Thr Glu Ser Leu Glu
260 265 270
Gln Gln Tyr Gln Val Val Lys Lys Arg Thr Ala Asn Gly Asp Ser Tyr
275 280 285
Tyr Gly Ser His Val Met Gln Tyr Gly Glu Leu Lys Leu Asp Leu Glu
290 295 300
Asn Leu Phe Leu Tyr Leu Gly Thr Asp Pro Ala Asn Asp Asn Tyr Thr
305 310 315 320
Phe Val Glu Gly Asn Ser Leu Ser Thr Ser Pro Ser Gly Arg Val Asn
325 330 335
Gln Arg Asp Ala Asp Leu Leu His Phe Trp His Lys Phe Trp Lys Ala
340 345 350
Pro Glu Arg Ser Ser Glu Lys Asp Glu Ala Gln Arg Arg Leu Ala Glu
355 360 365
Val Val Ser His Arg Ser His Ile Asp Asp Ser Ile Glu Leu Ile Gly
370 375 380
Asp Leu Leu Phe Gly Ser Thr Glu Gly Thr Arg Val Leu Lys His Val
385 390 395 400
Arg Pro Ser Gly Arg Pro Val Val Asp Asp Trp Asp Cys Leu Arg Ser
405 410 415
Leu Val Arg Thr Phe Glu Thr Tyr Cys Gly Ser Leu Ser Gln Tyr Gly
420 425 430
Met Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn Ser Gly Val Lys
435 440 445
Ala Glu Lys Met Gly Glu Ala Thr Gln Gln Val Cys Thr Ala Phe Pro
450 455 460
Ser His Asn Pro Trp Ser Ser Leu His Arg Gly Phe Ser Ala
465 470 475
<210> 66
<211> 486
<212> PRT
<213> Zea mays
<400> 66
Met Val Ala Ala Arg Leu Arg Leu Ala Leu Leu Leu Ser Val Cys Leu
1 5 10 15
Cys Ser Ala Trp Ala Arg Pro Arg Leu Glu Thr Ala Ile Arg Leu Pro
20 25 30
Ser Gln Arg Ala Ala Ala Ala Asp Glu Thr Asp Asp Gly Ala Val Gly
35 40 45
Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Ser Gly Tyr Tyr Asn Tyr
50 55 60
Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln Ile Met Lys Lys Gly
65 70 75 80
Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala
85 90 95
His Ser Pro Glu Asn Pro Arg Pro Gly Val Ile Ile Asn His Pro Gln
100 105 110
Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Arg Asp
115 120 125
Val Asn Val Asp Asn Phe Phe Ala Val Leu Leu Gly Asn Lys Thr Ala
130 135 140
Leu Arg Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asp Asp His
145 150 155 160
Ile Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met
165 170 175
Pro Thr Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asp Val Leu Lys
180 185 190
Lys Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu
195 200 205
Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Asn Asp Ile
210 215 220
Asn Val Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly
225 230 235 240
Thr Tyr Cys Pro Gly Glu Phe Pro Ser Pro Pro Pro Glu Tyr Asp Thr
245 250 255
Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Phe
260 265 270
His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Lys Leu Val Lys
275 280 285
Asp Arg Thr Ala Val His Asp Thr Phe Ser Tyr Gly Ser His Val Met
290 295 300
Gln Tyr Gly Ala Leu Glu Leu Asn Val Gln Arg Leu Phe Ser Tyr Ile
305 310 315 320
Gly Thr Asp Pro Ala Asn Asp Gly Asn Thr Phe Ile Glu Asp Asn Ser
325 330 335
Leu Pro Ser Phe Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val
340 345 350
Tyr Phe Trp Gln Lys Tyr Arg Lys Leu Ala Asp Ser Ser Pro Pro Lys
355 360 365
Ser Glu Ala Arg Lys Glu Leu Leu Glu Val Met Ala His Arg Ser His
370 375 380
Val Asp Ser Ser Val Glu Leu Ile Gly Ser Leu Leu Phe Gly Ser Glu
385 390 395 400
Asp Gly Pro Arg Val Leu Lys Ala Val Arg Ala Pro Gly Glu Pro Leu
405 410 415
Val Asp Asp Trp Ser Cys Leu Lys Ser Ile Val Arg Thr Phe Glu Ala
420 425 430
Arg Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg Ser Phe
435 440 445
Ala Asn Met Cys Asn Ala Gly Ile Leu Pro Glu Ala Val Ser Lys Val
450 455 460
Thr Ala Gln Ala Cys Ser Ser Ile Pro Ser Asn Pro Trp Ser Ser Ile
465 470 475 480
His Lys Gly Phe Ser Ala
485
<210> 67
<211> 486
<212> PRT
<213> Zea mays
<400> 67
Met Val Ala Ala Arg Leu Arg Leu Ser Leu Leu Leu Ser Val Cys Leu
1 5 10 15
Ser Ser Ala Trp Ala Arg Pro Arg Leu Glu Pro Ala Ile Arg Leu Pro
20 25 30
Ser Gln Arg Ala Ala Ala Ala Asp Glu Thr Asp Asp Gly Asp Val Gly
35 40 45
Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Ser Gly Tyr Tyr Asn Tyr
50 55 60
Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln Ile Met Lys Lys Gly
65 70 75 80
Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala
85 90 95
His Ser Pro Glu Asn Pro Arg Pro Gly Val Ile Ile Asn His Pro Gln
100 105 110
Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Arg Asp
115 120 125
Val Asn Val Asp Asn Phe Phe Ala Val Leu Leu Gly Asn Lys Thr Ala
130 135 140
Leu Arg Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His
145 150 155 160
Ile Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met
165 170 175
Pro Thr Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asp Val Leu Lys
180 185 190
Lys Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu
195 200 205
Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Asn Asp Ile
210 215 220
Asn Val Tyr Ala Thr Thr Ala Ser Asn Ala Asp Glu Ser Ser Trp Gly
225 230 235 240
Thr Tyr Cys Pro Gly Glu Phe Pro Ser Pro Pro Pro Glu Tyr Asp Thr
245 250 255
Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Phe
260 265 270
His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Lys Leu Val Lys
275 280 285
Asp Arg Thr Ala Val His Asp Thr Phe Ser Tyr Gly Ser His Val Met
290 295 300
Gln Tyr Gly Ala Leu Glu Leu Asn Val Gln Arg Leu Phe Ser Tyr Ile
305 310 315 320
Gly Thr Asp Pro Ala Asn Asp Gly Asn Thr Phe Ile Glu Asp Asn Ser
325 330 335
Leu Pro Ser Phe Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val
340 345 350
Tyr Phe Trp Gln Lys Tyr Arg Lys Leu Ala Asp Ser Ser His Ala Lys
355 360 365
Asn Glu Ala Arg Lys Glu Leu Leu Glu Val Met Ala His Arg Ser His
370 375 380
Val Asp Ser Ser Val Glu Leu Ile Gly Ser Leu Leu Phe Gly Ser Glu
385 390 395 400
Asp Gly Pro Arg Val Leu Lys Ala Val Arg Ala Pro Gly Glu Pro Leu
405 410 415
Val Asp Asp Trp Ser Cys Leu Lys Ser Ile Val Arg Thr Phe Glu Ala
420 425 430
Arg Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg Ser Phe
435 440 445
Ala Asn Met Cys Asn Ala Gly Ile Leu Pro Glu Ala Val Ser Lys Val
450 455 460
Ala Ala Gln Ala Cys Ser Ser Ile Pro Ser Asn Pro Trp Ser Ser Ile
465 470 475 480
His Lys Gly Phe Ser Ala
485
<210> 68
<211> 488
<212> PRT
<213> Saccharum officinarum
<400> 68
Met Val Thr Ala Arg Leu Arg Leu Ala Leu Leu Leu Leu Ser Val Phe
1 5 10 15
Leu Cys Ser Ala Trp Ala Arg Pro Arg Leu Glu Pro Thr Ile Arg Leu
20 25 30
Pro Ser Glu Arg Ala Ala Ala Ala Ala Gly Asp Glu Thr Asp Asp Ala
35 40 45
Val Gly Thr Arg Trp Ala Val Leu Val Ala Gly Ser Ser Gly Tyr Tyr
50 55 60
Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln Ile Met Lys
65 70 75 80
Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp
85 90 95
Ile Ala His Ser Ala Glu Asn Pro Arg Pro Gly Val Val Ile Asn His
100 105 110
Pro Gln Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly
115 120 125
Arg Gln Val Ser Val Asn Asn Phe Phe Ala Val Leu Leu Gly Asn Lys
130 135 140
Thr Ala Leu Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn
145 150 155 160
Asp His Ile Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu
165 170 175
Gly Met Pro Thr Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asp Val
180 185 190
Leu Lys Lys Lys His Ala Ala Gly Ser Tyr Lys Ser Leu Val Phe Tyr
195 200 205
Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Asp
210 215 220
Asp Ile Asn Val Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser
225 230 235 240
Trp Gly Thr Tyr Cys Pro Gly Glu Phe Pro Ser Pro Pro Pro Glu Tyr
245 250 255
Asp Thr Cys Leu Gly Asp Leu Tyr Ser Val Ser Trp Met Glu Asp Ser
260 265 270
Asp Phe His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Lys Leu
275 280 285
Val Lys Asp Arg Thr Ala Ala Gln Asp Thr Phe Ser Tyr Gly Ser His
290 295 300
Val Met Gln Tyr Gly Ser Leu Glu Leu Asn Val Gln Lys Leu Phe Ser
305 310 315 320
Tyr Ile Gly Thr Asn Pro Ala Asn Asp Gly Asn Thr Phe Val Glu Asp
325 330 335
Asn Ser Leu Pro Ser Phe Ser Lys Ala Val Asn Gln Arg Asp Ala Asp
340 345 350
Leu Val Tyr Phe Trp Gln Lys Tyr Arg Lys Leu Ala Asp Gly Ser Ser
355 360 365
Lys Lys Asn Glu Ala Arg Lys Glu Leu Leu Glu Val Met Ser His Arg
370 375 380
Ser His Val Asp Asn Ser Val Glu Leu Ile Gly Ser Leu Leu Phe Gly
385 390 395 400
Ser Glu Asp Gly Pro Arg Val Leu Lys Ala Val Arg Ala Ala Gly Glu
405 410 415
Pro Leu Val Asp Asp Trp Ser Cys Leu Lys Ser Met Val Arg Thr Phe
420 425 430
Glu Ala Gln Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg
435 440 445
Thr Phe Ala Asn Ile Cys Asn Ala Gly Ile Leu Pro Glu Ala Val Ser
450 455 460
Lys Val Ala Ala Gln Ala Cys Thr Ser Ile Pro Ser Asn Pro Trp Ser
465 470 475 480
Ser Ile Asp Lys Gly Phe Ser Ala
485
<210> 69
<211> 486
<212> PRT
<213> Zea mays
<400> 69
Met Val Ala Ala Arg Leu Arg Leu Ala Leu Leu Leu Ser Val Cys Leu
1 5 10 15
Cys Ser Ala Trp Ala Arg Pro Arg Leu Glu Thr Ala Ile Arg Leu Pro
20 25 30
Ser Gln Arg Ala Ala Ala Ala Asp Glu Thr Asp Asp Gly Ala Val Gly
35 40 45
Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Ser Gly Tyr Tyr Asn Tyr
50 55 60
Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln Ile Met Lys Lys Gly
65 70 75 80
Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala
85 90 95
His Ser Pro Glu Asn Pro Arg Pro Gly Val Ile Ile Asn His Pro Gln
100 105 110
Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Arg Asp
115 120 125
Val Asn Val Asp Asn Phe Phe Ala Val Leu Leu Gly Asn Lys Thr Ala
130 135 140
Leu Arg Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asp Asp His
145 150 155 160
Ile Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met
165 170 175
Pro Thr Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asp Val Leu Lys
180 185 190
Lys Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu
195 200 205
Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Asn Asp Ile
210 215 220
Asn Val Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly
225 230 235 240
Thr Tyr Cys Pro Gly Glu Phe Pro Ser Pro Pro Pro Glu Tyr Asp Thr
245 250 255
Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Phe
260 265 270
His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Lys Leu Val Lys
275 280 285
Asp Arg Thr Ala Val His Asp Thr Phe Ser Tyr Gly Ser His Val Met
290 295 300
Gln Tyr Gly Ala Leu Glu Leu Asn Val Gln His Leu Phe Ser Tyr Ile
305 310 315 320
Gly Thr Asp Pro Ala Asn Asp Gly Asn Thr Phe Ile Glu Asp Asn Ser
325 330 335
Leu Pro Ser Phe Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val
340 345 350
Tyr Phe Trp Gln Lys Tyr Arg Lys Phe Ala Asp Ser Pro Pro Ala Lys
355 360 365
Ser Glu Ala Arg Lys Glu Leu Leu Glu Val Met Ala His Arg Ser His
370 375 380
Val Asp Ser Ser Val Glu Leu Ile Gly Ser Leu Leu Phe Gly Ser Glu
385 390 395 400
Asp Gly Pro Arg Val Leu Lys Ala Val Arg Ala Pro Gly Glu Pro Leu
405 410 415
Val Asp Asp Trp Ser Cys Leu Lys Ser Ile Val Arg Thr Phe Glu Ala
420 425 430
Arg Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg Ser Phe
435 440 445
Ala Asn Met Cys Asn Ala Gly Ile Leu Pro Glu Ala Val Ser Lys Val
450 455 460
Ala Ala Gln Ala Cys Ser Ser Ile Pro Ser Asn Pro Trp Ser Ser Ile
465 470 475 480
His Lys Gly Phe Ser Ala
485
<210> 70
<211> 481
<212> PRT
<213> Sorghum bicolor
<400> 70
Met Val Ala Ala Arg Leu Arg Leu Ala Leu Leu Leu Leu Leu Pro Val
1 5 10 15
Phe Leu Cys Ser Ala Trp Ala Arg Pro Arg Leu Glu Pro Thr Ile Arg
20 25 30
Leu Pro Ser Asp Arg Ala Asp Asp Ala Val Gly Thr Arg Trp Ala Val
35 40 45
Leu Val Ala Gly Ser Asn Gly Tyr Tyr Asn Tyr Arg His Gln Ala Asp
50 55 60
Ile Cys His Ala Tyr Gln Ile Met Lys Lys Gly Gly Leu Lys Asp Glu
65 70 75 80
Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala His Ser Pro Glu Asn
85 90 95
Pro Arg Pro Gly Val Leu Ile Asn His Pro Gln Gly Gly Asp Val Tyr
100 105 110
Ala Gly Val Pro Lys Asp Tyr Thr Gly Arg Glu Val Ser Val Asn Asn
115 120 125
Phe Phe Ala Val Leu Leu Gly Asn Lys Thr Ala Leu Lys Gly Gly Ser
130 135 140
Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Val Phe Tyr
145 150 155 160
Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Tyr Pro Tyr
165 170 175
Leu Tyr Gly Asp Asp Leu Val Asp Val Leu Lys Lys Lys His Ala Ala
180 185 190
Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly
195 200 205
Ser Ile Phe Glu Gly Leu Leu Pro Asp Asp Ile Asn Val Tyr Ala Thr
210 215 220
Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly
225 230 235 240
Glu Phe Pro Ser Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly Asp Leu
245 250 255
Tyr Ser Val Ser Trp Met Glu Asp Ser Asp Phe His Asn Leu Arg Thr
260 265 270
Glu Ser Leu Lys Gln Gln Tyr Lys Leu Val Lys Asp Arg Thr Ala Val
275 280 285
Gln Asp Thr Phe Ser Tyr Gly Ser His Val Met Gln Tyr Gly Ser Leu
290 295 300
Glu Leu Asn Val Gln Lys Leu Phe Ser Tyr Ile Gly Thr Asn Pro Ala
305 310 315 320
Asn Asp Gly Asn Thr Phe Val Glu Asp Asn Ser Leu Pro Ser Phe Ser
325 330 335
Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val Tyr Phe Trp Gln Lys
340 345 350
Tyr Arg Lys Leu Ala Asp Asp Ser Ser Lys Lys Asn Glu Ala Arg Lys
355 360 365
Glu Leu Leu Glu Val Met Ala His Arg Ser His Val Asp Asn Ser Val
370 375 380
Glu Leu Ile Gly Ser Leu Leu Phe Gly Ser Glu Asp Gly Pro Arg Val
385 390 395 400
Leu Lys Ala Val Arg Ala Ala Gly Glu Pro Leu Val Asp Asp Trp Ser
405 410 415
Cys Leu Lys Ser Met Val Arg Thr Phe Glu Ala Gln Cys Gly Ser Leu
420 425 430
Ala Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn
435 440 445
Ala Gly Ile Leu Pro Glu Ala Val Ser Lys Val Ala Ala Gln Ala Cys
450 455 460
Thr Ser Ile Pro Ser Asn Pro Trp Ser Ser Ile Asp Lys Gly Phe Ser
465 470 475 480
Ala
<210> 71
<211> 488
<212> PRT
<213> Saccharum hybrid cultivar SP80-3280
<400> 71
Met Val Ala Ala Arg Leu Arg Leu Ala Leu Leu Leu Leu Ser Val Cys
1 5 10 15
Leu Cys Ser Ala Trp Ala Arg Pro Arg Leu Glu Pro Thr Ile Arg Leu
20 25 30
Pro Ser Glu Arg Ala Ala Ala Ala Ala Gly Asp Glu Thr Asp Asp Ala
35 40 45
Val Gly Thr Arg Trp Ala Val Leu Val Ala Gly Ser Ser Gly Tyr Tyr
50 55 60
Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln Ile Met Lys
65 70 75 80
Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp
85 90 95
Ile Ala His Ser Ala Glu Asn Pro Arg Pro Gly Val Val Ile Asn His
100 105 110
Pro Gln Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly
115 120 125
Arg Gln Val Ser Val Asn Asn Phe Phe Ala Val Leu Leu Gly Asn Lys
130 135 140
Thr Ala Leu Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn
145 150 155 160
Asp His Ile Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu
165 170 175
Gly Met Pro Thr Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asp Val
180 185 190
Leu Lys Lys Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr
195 200 205
Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Asp
210 215 220
Asp Ile Asn Val Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser
225 230 235 240
Trp Gly Thr Tyr Cys Pro Gly Glu Phe Pro Ser Pro Pro Pro Glu Tyr
245 250 255
Asp Thr Cys Leu Gly Asp Leu Tyr Ser Val Ser Trp Met Glu Asp Ser
260 265 270
Asp Phe His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Lys Leu
275 280 285
Val Lys Asp Arg Thr Ala Ala Gln Asp Thr Phe Ser Tyr Gly Ser His
290 295 300
Val Met Gln Tyr Gly Ser Leu Glu Leu Asn Val Gln Lys Leu Phe Ser
305 310 315 320
Tyr Ile Gly Thr Asn Pro Ala Asn Asp Gly Asn Thr Phe Val Glu Asp
325 330 335
Asn Ser Leu Pro Ser Phe Phe Lys Ser Cys Asn Gln Arg Asp Ala Asp
340 345 350
Leu Val Tyr Phe Trp Gln Lys Tyr Arg Lys Leu Ala Asp Gly Ser Ser
355 360 365
Lys Lys Asn Glu Ala Arg Lys Glu Leu Leu Glu Val Met Ser His Arg
370 375 380
Ser His Val Asp Asn Ser Val Glu Leu Ile Gly Ser Leu Leu Phe Gly
385 390 395 400
Ser Glu Asp Gly Pro Arg Val Leu Lys Ala Val Arg Ala Ala Gly Glu
405 410 415
Pro Leu Val Asp Asp Trp Ser Cys Leu Lys Ser Met Val Arg Thr Phe
420 425 430
Glu Ala Gln Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg
435 440 445
Thr Phe Ala Asn Ile Cys Asn Ala Gly Ile Leu Pro Glu Ala Val Ser
450 455 460
Lys Val Ala Ala Gln Ala Cys Thr Ser Ile Pro Ser Asn Pro Trp Ser
465 470 475 480
Ser Ile Asp Lys Gly Phe Ser Ala
485
<210> 72
<211> 503
<212> PRT
<213> Oryza sativa Japonica Group
<400> 72
Met Ala Ala Arg Ala Ser Ala Phe Arg Leu Val Leu Pro Pro Ala Arg
1 5 10 15
Gly Ala Ala Pro Ser Phe Ala His Leu Ala Ala Val Ala Val Ala Arg
20 25 30
Pro Arg Trp Glu Glu Glu Gly Ser Asn Leu Arg Leu Pro Ser Glu Arg
35 40 45
Ala Val Ala Ala Gly Ala Ala Ala Asp Asp Ala Ala Glu Ala Ala Glu
50 55 60
Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Tyr Asn
65 70 75 80
Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Ile Met Lys Arg
85 90 95
Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile
100 105 110
Ala His Asn Pro Glu Asn Pro Arg Pro Gly Val Ile Ile Asn His Pro
115 120 125
Gln Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Lys
130 135 140
Glu Val Asn Val Lys Asn Leu Phe Ala Val Leu Leu Gly Asn Lys Thr
145 150 155 160
Ala Val Lys Gly Gly Ser Gly Lys Val Leu Asp Ser Gly Pro Asn Asp
165 170 175
His Ile Phe Ile Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly
180 185 190
Met Pro Thr Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asp Val Leu
195 200 205
Lys Lys Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu
210 215 220
Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Asn Gly
225 230 235 240
Ile Asn Val Tyr Ala Thr Thr Ala Ser Asn Ala Asp Glu Ser Ser Trp
245 250 255
Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Asp
260 265 270
Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp
275 280 285
Val His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Asn Leu Val
290 295 300
Lys Glu Arg Thr Ser Val Gln His Thr Tyr Tyr Ser Gly Ser His Val
305 310 315 320
Met Glu Tyr Gly Ser Leu Glu Leu Asn Ala His His Val Phe Met Tyr
325 330 335
Met Gly Ser Asn Pro Ala Asn Asp Asn Ala Thr Phe Val Glu Asp Asn
340 345 350
Ser Leu Pro Ser Phe Ser Arg Ala Val Asn Gln Arg Asp Ala Asp Leu
355 360 365
Val Tyr Phe Trp Gln Lys Tyr Arg Lys Leu Pro Glu Ser Ser Pro Glu
370 375 380
Lys Asn Glu Ala Arg Lys Gln Leu Leu Glu Met Met Ala His Arg Ser
385 390 395 400
His Val Asp Asn Ser Val Glu Leu Ile Gly Asn Leu Leu Phe Gly Ser
405 410 415
Glu Glu Gly Pro Arg Val Leu Lys Ala Val Arg Ala Thr Gly Glu Pro
420 425 430
Leu Val Asp Asp Trp Ser Cys Leu Lys Ser Met Val Arg Thr Phe Glu
435 440 445
Ala Gln Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg Ser
450 455 460
Phe Ala Asn Ile Cys Asn Ala Gly Ile Ser Ala Glu Ala Met Ala Lys
465 470 475 480
Val Ala Ala Gln Ala Cys Thr Ser Ile Pro Ser Asn Pro Trp Ser Ser
485 490 495
Thr His Arg Gly Phe Ser Ala
500
<210> 73
<211> 481
<212> PRT
<213> Zea mays
<400> 73
Met Val Ala Asp Arg Leu Arg Leu Ala Leu Leu Leu Ser Ala Cys Leu
1 5 10 15
Cys Ser Ala Trp Ala Arg Pro Arg Leu Glu Pro Thr Ile Arg Leu Pro
20 25 30
Ser Glu Arg Ala Ala Ala Asp Asp Ala Val Gly Thr Arg Trp Ala Val
35 40 45
Leu Ile Ala Gly Ser Asn Gly Tyr Tyr Asn Tyr Arg His Gln Ala Asp
50 55 60
Ile Cys His Ala Tyr Gln Ile Met Lys Lys Gly Gly Leu Lys Asp Glu
65 70 75 80
Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala His Ser Pro Glu Asn
85 90 95
Pro Arg Pro Gly Val Ile Ile Asn His Pro Gln Gly Gly Asp Val Tyr
100 105 110
Ala Gly Val Pro Lys Asp Tyr Thr Gly Arg Glu Val Asn Val Asp Asn
115 120 125
Phe Phe Ala Val Leu Leu Gly Asn Lys Thr Ala Leu Arg Gly Gly Ser
130 135 140
Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Val Phe Tyr
145 150 155 160
Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Tyr Pro Tyr
165 170 175
Leu Tyr Gly Asp Asp Leu Val Asp Val Leu Lys Lys Lys His Ala Ala
180 185 190
Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly
195 200 205
Ser Ile Phe Glu Gly Leu Leu Pro Asn Asp Ile Asn Val Tyr Ala Thr
210 215 220
Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly
225 230 235 240
Glu Phe Pro Ser Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly Asp Leu
245 250 255
Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Phe His Asn Leu Arg Thr
260 265 270
Glu Ser Leu Lys Gln Gln Tyr Asn Leu Val Lys Asp Arg Thr Ala Val
275 280 285
Gln Asp Thr Phe Ser Tyr Gly Ser His Val Met Gln Tyr Gly Ser Leu
290 295 300
Glu Leu Asn Val Lys His Leu Phe Ser Tyr Ile Gly Thr Asn Pro Ala
305 310 315 320
Asn Asp Asp Asn Thr Phe Ile Glu Asp Asn Ser Leu Pro Ser Phe Ser
325 330 335
Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val Tyr Phe Trp Gln Lys
340 345 350
Tyr Arg Lys Leu Ala Asp Ser Ser Pro Glu Lys Asn Glu Ala Arg Lys
355 360 365
Glu Leu Leu Glu Val Met Ala His Arg Ser His Val Asp Ser Ser Val
370 375 380
Glu Leu Ile Gly Ser Leu Leu Phe Gly Ser Glu Asp Gly Pro Arg Val
385 390 395 400
Leu Lys Ala Val Arg Ala Ala Gly Glu Pro Leu Val Asp Asp Trp Ser
405 410 415
Cys Leu Lys Ser Thr Val Arg Thr Phe Glu Ala Gln Cys Gly Ser Leu
420 425 430
Ala Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn
435 440 445
Ala Gly Ile Leu Pro Glu Ala Val Ser Lys Val Ala Ala Gln Ala Cys
450 455 460
Thr Ser Ile Pro Ser Asn Pro Trp Ser Ser Ile His Lys Gly Phe Ser
465 470 475 480
Ala
<210> 74
<211> 481
<212> PRT
<213> Zea mays
<400> 74
Met Val Ala Asp Arg Leu Arg Leu Ala Leu Leu Leu Ser Ala Cys Leu
1 5 10 15
Cys Ser Ala Trp Ala Arg Pro Arg Leu Glu Pro Thr Ile Arg Leu Pro
20 25 30
Ser Asp Arg Ala Ala Ala Asp Asp Ala Val Gly Thr Arg Trp Ala Val
35 40 45
Leu Ile Ala Gly Ser Asn Gly Tyr Tyr Asn Tyr Arg His Gln Ala Asp
50 55 60
Ile Cys His Ala Tyr Gln Ile Met Lys Lys Gly Gly Leu Lys Asp Glu
65 70 75 80
Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala His Ser Pro Glu Asn
85 90 95
Pro Arg Pro Gly Val Ile Ile Asn His Pro Gln Gly Gly Asp Val Tyr
100 105 110
Ala Gly Val Pro Lys Asp Tyr Thr Gly Arg Asp Val Asn Val Asp Asn
115 120 125
Phe Phe Ala Val Leu Leu Gly Asn Lys Thr Ala Leu Arg Gly Gly Ser
130 135 140
Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Ser Val Phe Tyr
145 150 155 160
Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Tyr Pro Tyr
165 170 175
Leu Tyr Gly Asp Asp Leu Val Asp Val Leu Lys Lys Lys His Ala Ala
180 185 190
Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly
195 200 205
Ser Ile Phe Glu Gly Leu Leu Pro Asn Asp Ile Asn Val Tyr Ala Thr
210 215 220
Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly
225 230 235 240
Glu Phe Pro Ser Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly Asp Leu
245 250 255
Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Phe His Asn Leu Arg Thr
260 265 270
Glu Ser Leu Lys Gln Gln Tyr Asn Leu Val Lys Asp Arg Thr Ala Val
275 280 285
Gln Asp Thr Phe Ser Tyr Gly Ser His Val Met Gln Tyr Gly Ser Leu
290 295 300
Gly Leu Asn Val Lys His Leu Phe Ser Tyr Ile Gly Thr Asn Pro Ala
305 310 315 320
Asn Asp Asp Asn Thr Phe Ile Glu Asp Asn Ser Leu Pro Ser Phe Ser
325 330 335
Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val Tyr Phe Trp Gln Lys
340 345 350
Tyr Arg Lys Leu Ala Asp Ser Ser Pro Glu Lys Asn Glu Ala Arg Arg
355 360 365
Glu Leu Leu Glu Val Met Ala His Arg Ser His Val Asp Ser Ser Val
370 375 380
Glu Leu Ile Gly Ser Leu Leu Phe Gly Ser Glu Asp Gly Pro Arg Val
385 390 395 400
Leu Lys Ala Val Arg Ala Ala Gly Glu Pro Leu Val Asp Asp Trp Ser
405 410 415
Cys Leu Lys Ser Thr Val Arg Thr Phe Glu Ala Gln Cys Gly Ser Leu
420 425 430
Ala Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn
435 440 445
Ala Gly Ile Leu Pro Glu Ala Val Ser Lys Val Ala Ala Gln Ala Cys
450 455 460
Thr Ser Ile Pro Ser Asn Pro Trp Ser Ser Ile His Lys Gly Phe Ser
465 470 475 480
Ala
<210> 75
<211> 501
<212> PRT
<213> Oryza sativa Japonica Group
<400> 75
Met Ala Ala Arg Ala Arg Leu Arg Leu Val Leu Pro Pro Leu Ala Ala
1 5 10 15
Leu Leu Leu Phe Ala His Leu Ala Ala Val Ala Val Ala Arg Pro Arg
20 25 30
Trp Glu Glu Glu Gly Ser Asn Leu Arg Leu Pro Ser Glu Arg Ala Val
35 40 45
Ala Ala Gly Ala Ala Ala Asp Asp Ala Ala Glu Ala Ala Glu Gly Thr
50 55 60
Arg Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Tyr Asn Tyr Arg
65 70 75 80
His Gln Ala Asp Val Cys His Ala Tyr Gln Ile Met Lys Arg Gly Gly
85 90 95
Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala His
100 105 110
Asn Pro Glu Asn Pro Arg Pro Gly Val Ile Ile Asn His Pro Gln Gly
115 120 125
Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Lys Glu Val
130 135 140
Asn Val Lys Asn Leu Phe Ala Val Leu Leu Gly Asn Lys Thr Ala Val
145 150 155 160
Lys Gly Gly Ser Gly Lys Val Leu Asp Ser Gly Pro Asn Asp His Ile
165 170 175
Phe Ile Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro
180 185 190
Thr Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asp Val Leu Lys Lys
195 200 205
Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala
210 215 220
Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Asn Gly Ile Asn
225 230 235 240
Val Tyr Ala Thr Thr Ala Ser Asn Ala Asp Glu Ser Ser Trp Gly Thr
245 250 255
Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Asp Thr Cys
260 265 270
Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Val His
275 280 285
Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Asn Leu Val Lys Glu
290 295 300
Arg Thr Ser Val Gln His Thr Tyr Tyr Ser Gly Ser His Val Met Glu
305 310 315 320
Tyr Gly Ser Leu Glu Leu Asn Ala His His Val Phe Met Tyr Met Gly
325 330 335
Ser Asn Pro Ala Asn Asp Asn Ala Thr Phe Val Glu Asp Asn Ser Leu
340 345 350
Pro Ser Phe Ser Arg Ala Val Asn Gln Arg Asp Ala Asp Leu Val Tyr
355 360 365
Phe Trp Gln Lys Tyr Arg Lys Leu Pro Glu Ser Ser Pro Glu Lys Asn
370 375 380
Glu Ala Arg Lys Gln Leu Leu Glu Met Met Ala His Arg Ser His Val
385 390 395 400
Asp Asn Ser Val Glu Leu Ile Gly Asn Leu Leu Phe Gly Ser Glu Glu
405 410 415
Gly Pro Arg Val Leu Lys Ala Val Arg Ala Thr Gly Glu Pro Leu Val
420 425 430
Asp Asp Trp Ser Cys Leu Lys Ser Met Val Arg Thr Phe Glu Ala Gln
435 440 445
Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala
450 455 460
Asn Ile Cys Asn Ala Gly Ile Ser Ala Glu Ala Met Ala Lys Val Ala
465 470 475 480
Ala Gln Ala Cys Thr Ser Ile Pro Ser Asn Pro Trp Ser Ser Thr His
485 490 495
Arg Gly Phe Ser Ala
500
<210> 76
<211> 485
<212> PRT
<213> Zea mays
<400> 76
Met Val Ala Asp Arg Leu Arg Leu Ala Leu Leu Leu Ser Ala Cys Leu
1 5 10 15
Cys Ser Ala Trp Ala Arg Pro Arg Leu Glu Pro Thr Ile Arg Leu Pro
20 25 30
Ser Glu Arg Ala Ala Ala Asp Glu Thr Asp Asp Asp Ala Val Gly Thr
35 40 45
Arg Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Tyr Asn Tyr Arg
50 55 60
His Gln Ala Asp Ile Cys His Ala Tyr Gln Ile Met Lys Lys Gly Gly
65 70 75 80
Leu Lys Asp Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala His
85 90 95
Ser Pro Glu Asn Pro Arg Pro Gly Val Ile Ile Asn His Pro Gln Gly
100 105 110
Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Arg Asp Val
115 120 125
Asn Val Asp Asn Phe Phe Ala Val Leu Leu Gly Asn Lys Thr Ala Leu
130 135 140
Arg Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile
145 150 155 160
Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro
165 170 175
Thr Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asp Val Leu Lys Lys
180 185 190
Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala
195 200 205
Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Asn Asp Ile Asn
210 215 220
Val Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr
225 230 235 240
Tyr Cys Pro Gly Glu Phe Pro Ser Pro Pro Pro Glu Tyr Asp Thr Cys
245 250 255
Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Phe His
260 265 270
Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Asn Leu Val Lys Asp
275 280 285
Arg Thr Ala Val Gln Asp Thr Phe Ser Tyr Gly Ser His Val Met Gln
290 295 300
Tyr Gly Ser Leu Glu Leu Asn Val Lys His Leu Phe Ser Tyr Ile Gly
305 310 315 320
Thr Asn Pro Ala Asn Asp Asp Asn Thr Phe Ile Glu Asp Asn Ser Leu
325 330 335
Pro Ser Leu Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val Tyr
340 345 350
Phe Trp Gln Lys Tyr Arg Lys Leu Ala Asp Ser Ser Pro Glu Lys Asn
355 360 365
Glu Ala Arg Arg Glu Leu Leu Glu Val Met Ala His Arg Ser His Val
370 375 380
Asp Ser Ser Val Glu Leu Ile Gly Ser Leu Leu Phe Gly Ser Glu Asp
385 390 395 400
Gly Pro Arg Val Leu Lys Ala Val Arg Ala Ala Gly Glu Pro Leu Val
405 410 415
Asp Asp Trp Ser Cys Leu Lys Ser Thr Val Arg Thr Phe Glu Ala Gln
420 425 430
Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala
435 440 445
Asn Ile Cys Asn Ala Gly Ile Leu Pro Glu Ala Val Ser Lys Val Ala
450 455 460
Ala Gln Ala Cys Thr Ser Ile Pro Ser Asn Pro Trp Ser Ser Ile His
465 470 475 480
Lys Gly Phe Ser Ala
485
<210> 77
<211> 488
<212> PRT
<213> Brachypodium distachyon
<400> 77
Met Ala Ser Phe Arg Leu Leu Pro Leu Ala Leu Leu Leu Cys Ala Cys
1 5 10 15
Leu Ser Ala His Ala Arg Thr Ser Leu Leu Glu Gln Thr Ile Arg Leu
20 25 30
Pro Ser Gln Arg Gly Ala Ala Gly Gln Gln Glu Val Asp Asp Asp Ser
35 40 45
Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Tyr
50 55 60
Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln Ile Met Lys
65 70 75 80
Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp
85 90 95
Ile Ala His Asn Pro Glu Asn Pro Arg Pro Gly Val Ile Ile Asn His
100 105 110
Pro Gln Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly
115 120 125
Lys Glu Val Asn Val Lys Asn Phe Phe Ala Val Leu Leu Gly Asn Lys
130 135 140
Ala Ala Val Ser Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn
145 150 155 160
Asp His Ile Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu
165 170 175
Gly Met Pro Thr Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asp Val
180 185 190
Leu Lys Lys Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr
195 200 205
Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Asn
210 215 220
Asp Ile Gly Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser
225 230 235 240
Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr
245 250 255
Asp Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ala Trp Met Glu Asp Ser
260 265 270
Asp Val His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Asp Leu
275 280 285
Val Lys Lys Arg Thr Ala Pro Glu Asn Ser Tyr Ser Tyr Gly Ser His
290 295 300
Val Met Gln Tyr Gly Ser Leu Asp Leu Asn Ala Glu His Leu Phe Leu
305 310 315 320
Tyr Ile Gly Ser Asn Pro Ala Asn Asp Asn Thr Thr Phe Val Glu Gly
325 330 335
Asn Ser Leu Pro Ser Phe Ser Arg Ala Val Asn Gln Arg Asp Ala Asp
340 345 350
Leu Val Tyr Phe Trp Gln Lys Tyr Arg Lys Leu Ala Glu Ser Ser Pro
355 360 365
Ala Lys Asn Asp Ala Arg Lys Glu Leu Leu Glu Met Met Ala His Arg
370 375 380
Ser His Val Asp Asn Ser Val Glu Leu Thr Gly Asn Leu Leu Phe Gly
385 390 395 400
Ser Glu Asp Gly Pro Met Val Leu Lys Thr Val Arg Thr Ala Gly Glu
405 410 415
Pro Leu Val Asp Asp Trp Gly Cys Leu Lys Ser Thr Val Arg Ala Phe
420 425 430
Glu Ser Gln Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg
435 440 445
Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile Leu Pro Glu Ala Thr Ala
450 455 460
Lys Val Ala Ala Gln Ala Cys Pro Ser Ile Pro Ala Asn Pro Trp Ser
465 470 475 480
Ala Thr His Lys Gly Phe Ser Ala
485
<210> 78
<211> 484
<212> PRT
<213> Hordeum vulgare subsp. vulgare
<400> 78
Met Ala Met Ala Ser Phe Arg Leu Leu Pro Leu Ala Leu Leu Leu Ser
1 5 10 15
Val Ala His Ala Arg Thr Pro Arg Leu Glu Pro Thr Ile Arg Leu Pro
20 25 30
Ser Gln Arg Ala Ala Gly Gln Glu Asp Asp Asp Ser Val Gly Thr Arg
35 40 45
Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Tyr Asn Tyr Arg His
50 55 60
Gln Ala Asp Ile Cys His Ala Tyr Gln Ile Met Lys Lys Gly Gly Leu
65 70 75 80
Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Arg Asn
85 90 95
Pro Glu Asn Pro Arg Pro Gly Val Ile Ile Asn His Pro Gln Gly Gly
100 105 110
Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Lys Glu Val Asn
115 120 125
Val Lys Asn Phe Phe Ala Val Leu Leu Gly Asn Lys Thr Ala Val Asn
130 135 140
Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe
145 150 155 160
Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr
165 170 175
Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asp Val Leu Lys Lys Lys
180 185 190
His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys
195 200 205
Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Asn Asp Ile Gly Val
210 215 220
Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr
225 230 235 240
Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Asp Thr Cys Leu
245 250 255
Gly Asp Leu Tyr Ser Ile Ser Trp Met Glu Asp Ser Asp Val His Asn
260 265 270
Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Asn Leu Val Lys Lys Arg
275 280 285
Thr Ala Ala Gln Asp Ser Tyr Ser Tyr Gly Ser His Val Met Gln Tyr
290 295 300
Gly Ser Leu Asp Leu Asn Ala Glu His Leu Phe Ser Tyr Ile Gly Ser
305 310 315 320
Asn Pro Ala Asn Glu Asn Thr Thr Phe Val Glu Asp Asn Ala Leu Pro
325 330 335
Ser Leu Ser Arg Ala Val Asn Gln Arg Asp Ala Asp Leu Val Tyr Phe
340 345 350
Trp Gln Lys Tyr Arg Lys Leu Ala Glu Ser Ser Pro Ala Lys Asn Asn
355 360 365
Ala Arg Lys Gln Leu Leu Glu Met Met Gly His Arg Ser His Ile Asp
370 375 380
Ser Ser Val Glu Leu Ile Gly Asn Leu Leu Phe Gly Ser Ala Gly Gly
385 390 395 400
Pro Met Val Leu Lys Thr Val Arg Pro Ala Gly Glu Pro Leu Val Asp
405 410 415
Asp Trp Ser Cys Leu Lys Ser Thr Val Arg Thr Phe Glu Ser Gln Cys
420 425 430
Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn
435 440 445
Met Cys Asn Ala Gly Ile Val Pro Glu Ala Met Ala Lys Val Ala Ala
450 455 460
Gln Ala Cys Thr Ser Phe Pro Thr Asn Pro Trp Ser Ala Thr His Lys
465 470 475 480
Gly Phe Ser Ala
<210> 79
<211> 493
<212> PRT
<213> Aegilops tauschii
<400> 79
Met Ala Met Ala Ser Phe Arg Pro Leu Pro Leu Ala Leu Leu Leu Ala
1 5 10 15
Ala Cys Leu Ser Ala Phe Val Leu Ala Val Ala His Ala Arg Thr Pro
20 25 30
Arg Leu Glu Pro Thr Ile Arg Leu Pro Ser Gln Arg Ala Ala Gly Gln
35 40 45
Glu Asp Asp Asp Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly
50 55 60
Ser Asn Gly Tyr Tyr Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala
65 70 75 80
Tyr Gln Ile Met Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val
85 90 95
Phe Met Tyr Asp Asp Ile Ala His Asn Pro Glu Asn Pro Arg Pro Gly
100 105 110
Val Ile Ile Asn His Pro Gln Gly Gly Asp Val Tyr Ala Gly Val Pro
115 120 125
Lys Asp Tyr Thr Gly Lys Glu Val Asn Val Lys Asn Phe Phe Ala Val
130 135 140
Leu Leu Gly Asn Lys Thr Ala Val Ser Gly Gly Ser Gly Lys Val Val
145 150 155 160
Asp Ser Gly Pro Asn Asp His Ile Phe Val Phe Tyr Ser Asp His Gly
165 170 175
Gly Pro Gly Val Leu Gly Met Pro Thr Tyr Pro Tyr Leu Tyr Gly Asp
180 185 190
Asp Leu Val Asp Val Leu Lys Lys Lys His Ala Ala Gly Thr Tyr Lys
195 200 205
Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu
210 215 220
Gly Leu Leu Pro Asn Asp Ile Gly Val Tyr Ala Thr Thr Ala Ser Asn
225 230 235 240
Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser
245 250 255
Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ser
260 265 270
Trp Met Glu Asp Ser Asp Val His Asn Leu Arg Thr Glu Ser Leu Lys
275 280 285
Gln Gln Tyr Asn Leu Val Lys Lys Arg Thr Ala Ala Gln Asp Ser Tyr
290 295 300
Ser Tyr Gly Ser His Val Met Gln Tyr Gly Ser Leu Asp Leu Asn Ala
305 310 315 320
Glu His Leu Phe Ser Tyr Ile Gly Ser Asn Pro Ala Asn Glu Asn Thr
325 330 335
Thr Phe Val Glu Asp Asn Ala Leu Pro Ser Phe Ser Arg Ala Val Asn
340 345 350
Gln Arg Asp Ala Asp Leu Val Tyr Phe Trp Gln Lys Tyr Arg Lys Leu
355 360 365
Ala Glu Ser Ser Pro Glu Lys Asn Asp Ala Arg Lys Gln Leu Leu Glu
370 375 380
Met Met Gly His Arg Ser His Ile Asp Asn Ser Val Glu Pro Ile Gly
385 390 395 400
Asn Leu Leu Phe Gly Ser Ala Gly Gly Pro Met Val Leu Lys Ala Val
405 410 415
Arg Pro Ala Gly Glu Pro Leu Val Asp Asp Trp Ser Cys Leu Lys Ser
420 425 430
Thr Val Arg Thr Phe Glu Ser Gln Cys Gly Ser Leu Ala Gln Tyr Gly
435 440 445
Met Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile Val
450 455 460
Pro Glu Ala Met Ala Lys Val Ala Ala Gln Ala Arg Thr Ser Ile Pro
465 470 475 480
Thr Asn Pro Trp Ser Ala Thr His Lys Gly Phe Ser Ala
485 490
<210> 80
<211> 493
<212> PRT
<213> Triticum monococcum
<400> 80
Met Ala Met Ala Ser Phe Arg Pro Leu Pro Leu Ala Leu Leu Leu Ala
1 5 10 15
Ala Cys Leu Ser Ala Leu Val Leu Ala Val Ala His Ala Arg Ser Pro
20 25 30
Arg Leu Glu Pro Thr Ile Arg Leu Pro Ser Gln Arg Ala Ala Gly Gln
35 40 45
Glu Asp Asp Asp Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly
50 55 60
Ser Asn Gly Tyr Tyr Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala
65 70 75 80
Tyr Gln Ile Met Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val
85 90 95
Phe Met Tyr Asp Asp Ile Ala His Asn Leu Glu Asn Pro Gly Pro Gly
100 105 110
Val Ile Ile Asn His Pro Gln Gly Gly Asp Val Tyr Ala Gly Val Pro
115 120 125
Lys Asp Tyr Thr Gly Lys Glu Val Asn Val Lys Asn Leu Phe Ala Val
130 135 140
Leu Leu Gly Asn Lys Thr Ala Val Ser Gly Gly Ser Gly Lys Val Val
145 150 155 160
Asp Ser Gly Pro Asn Asp His Ile Phe Val Phe Tyr Ser Asp His Gly
165 170 175
Gly Pro Gly Val Leu Gly Met Pro Thr Tyr Pro Tyr Leu Tyr Gly Asp
180 185 190
Asp Leu Val Asp Val Leu Lys Lys Lys His Ala Ala Gly Thr Tyr Lys
195 200 205
Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu
210 215 220
Gly Leu Leu Pro Asn Asp Ile Gly Val Tyr Ala Thr Thr Ala Ser Asn
225 230 235 240
Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser
245 250 255
Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ser
260 265 270
Trp Met Glu Asp Ser Asp Val His Asn Leu Arg Thr Glu Ser Leu Lys
275 280 285
Gln Gln Tyr Asn Leu Val Lys Lys Arg Thr Ala Ala Gln Asp Ser Tyr
290 295 300
Ser Tyr Gly Ser His Val Met Gln Tyr Gly Ser Leu Asp Leu Asn Ala
305 310 315 320
Glu His Leu Phe Ser Tyr Ile Gly Ser Asn Pro Ala Asn Glu Asn Thr
325 330 335
Thr Phe Val Glu Asp Asn Ala Leu Pro Ser Phe Ser Arg Ala Val Asn
340 345 350
Gln Arg Asp Ala Asp Leu Val Tyr Phe Trp Gln Lys Tyr Arg Lys Leu
355 360 365
Ala Glu Ser Ser Pro Glu Lys Asn Asp Ala Arg Lys Gln Leu Leu Glu
370 375 380
Met Met Gly His Arg Ser His Ile Asp Asn Ser Val Glu Leu Ile Gly
385 390 395 400
Asn Leu Leu Phe Gly Ser Ala Gly Gly Pro Met Val Leu Lys Ala Val
405 410 415
Arg Pro Ala Gly Glu Pro Leu Val Asp Asp Trp Ser Cys Leu Lys Ser
420 425 430
Thr Val Arg Thr Phe Glu Ser Gln Cys Gly Ser Leu Ala Gln Tyr Gly
435 440 445
Met Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile Val
450 455 460
Pro Glu Ala Thr Ala Lys Val Ala Ala Gln Ala Cys Thr Ser Ile Pro
465 470 475 480
Thr Asn Pro Trp Ser Ala Thr His Lys Gly Phe Ser Ala
485 490
<210> 81
<211> 493
<212> PRT
<213> Aegilops speltoides
<400> 81
Met Ala Met Ala Ser Phe Arg Pro Leu Pro Leu Ala Leu Leu Leu Ala
1 5 10 15
Ala Cys Leu Ser Glu Leu Val Leu Ala Val Ala His Ala Arg Thr Pro
20 25 30
Arg Leu Glu Pro Thr Ile Arg Leu Pro Ser Gln Arg Ala Ala Gly Gln
35 40 45
Glu Asp Asp Asp Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly
50 55 60
Ser Asn Gly Tyr Tyr Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala
65 70 75 80
Tyr Gln Ile Met Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Ile
85 90 95
Phe Met Tyr Asp Asp Ile Ala His Asn Pro Glu Asn Pro Arg Pro Gly
100 105 110
Val Ile Ile Asn His Pro Gln Gly Gly Asp Val Tyr Ala Gly Val Pro
115 120 125
Lys Asp Tyr Thr Gly Lys Glu Val Asn Val Lys Asn Phe Phe Ala Val
130 135 140
Leu Leu Gly Asn Arg Thr Ala Val Ser Gly Gly Ser Gly Lys Val Val
145 150 155 160
Asp Ser Gly Pro Asn Asp His Ile Phe Val Phe Tyr Ser Asp His Gly
165 170 175
Gly Pro Gly Val Leu Gly Met Pro Thr Tyr Pro Tyr Leu Tyr Gly Asp
180 185 190
Asp Leu Val Asp Val Leu Lys Lys Lys His Ala Ala Gly Thr Tyr Lys
195 200 205
Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu
210 215 220
Gly Leu Leu Pro Asn Asp Ile Gly Val Tyr Ala Thr Thr Ala Ser Asn
225 230 235 240
Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser
245 250 255
Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ser
260 265 270
Trp Met Glu Asp Ser Asp Val His Asn Leu Arg Thr Glu Ser Leu Lys
275 280 285
Gln Gln Tyr Asn Leu Val Lys Lys Arg Thr Ala Ala Gln Asp Ser Tyr
290 295 300
Ser Tyr Gly Ser His Val Met Gln Tyr Gly Ser Leu Asp Leu Asn Ala
305 310 315 320
Glu His Leu Phe Ser Tyr Ile Gly Ser Asn Pro Ala Asn Glu Asn Thr
325 330 335
Thr Phe Val Glu Asp Asn Ala Leu Pro Ser Phe Ser Gly Ala Val Asn
340 345 350
Gln Arg Asp Ala Asp Leu Val Tyr Phe Trp Gln Lys Tyr Arg Lys Leu
355 360 365
Ala Glu Ser Ser Pro Glu Lys Asn Asp Ala Arg Lys Gln Leu Leu Glu
370 375 380
Met Met Gly His Arg Ser His Ile Asp Asn Ser Val Glu Leu Ile Gly
385 390 395 400
Asn Leu Leu Phe Gly Ser Ala Gly Gly Pro Met Val Leu Lys Ala Val
405 410 415
Arg Pro Ala Gly Glu Pro Leu Val Asp Asp Trp Ser Cys Leu Lys Ser
420 425 430
Thr Val Arg Thr Phe Glu Ser Gln Cys Gly Ser Leu Ala Gln Tyr Gly
435 440 445
Met Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn Val Gly Ile Val
450 455 460
Pro Glu Ala Met Ala Lys Val Ala Ala Gln Ala Cys Thr Asn Ile Pro
465 470 475 480
Thr Asn Pro Trp Ser Ala Thr His Lys Gly Phe Ser Ala
485 490
<210> 82
<211> 469
<212> PRT
<213> Amborella trichopoda
<400> 82
Met Ala Phe Ser Gly Lys Ser Val Leu Phe Leu Ala Val Phe Met Ala
1 5 10 15
Phe Ser Gly Val Tyr Gly Arg Tyr Ser Thr Trp Ser Asp Phe Leu Arg
20 25 30
Met Pro Gln Thr Glu Asp Ser Val Gly Thr Arg Trp Ala Val Leu Val
35 40 45
Ala Gly Ser Ser Gly Tyr Gly Asn Tyr Arg His Gln Ala Asp Ile Cys
50 55 60
His Ala Tyr Gln Thr Met Ile Arg Gly Gly Leu Lys Glu Lys Asn Ile
65 70 75 80
Val Val Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Glu Asn Pro Arg
85 90 95
Pro Gly Val Ile Ile Asn Arg Pro His Gly Glu Asp Val Tyr Ala Gly
100 105 110
Val Pro Lys Asp Tyr Val Gly Asp Asp Val Asn Val Asp Asn Leu Phe
115 120 125
Ala Val Ile Leu Gly Asn Lys Ser Ala Leu Thr Gly Gly Ser Gly Lys
130 135 140
Val Val Asp Ser Gly Pro Asp Asp His Ile Phe Ile Phe Tyr Ser Asp
145 150 155 160
His Gly Gly Ala Gly Val Leu Gly Met Pro Thr Tyr Pro Tyr Leu Tyr
165 170 175
Ala Asp Asp Leu Val Asn Val Leu Lys Lys Lys His Val Ser Gly Thr
180 185 190
Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile
195 200 205
Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala
210 215 220
Ser Asn Ala Val Glu Ser Ser Trp Gly Thr Tyr Cys Pro Asp Asp Ser
225 230 235 240
Pro Asp Phe Pro Gln Glu Tyr Asp Thr Cys Leu Gly Asp Leu Tyr Ser
245 250 255
Val Ser Trp Met Glu Asp Ser Asp Ile His Asn Leu Gln Phe Glu Thr
260 265 270
Leu Lys Gln Gln Tyr Glu Leu Val Lys Met Arg Thr Ser Asn Phe Glu
275 280 285
Thr Tyr Met Phe Gly Ser His Val Met Gln Tyr Gly Asp Ser Gly Leu
290 295 300
Gly Lys Glu Gln Leu Val Leu Tyr Met Gly Ser Asn Pro Ala Asn Asp
305 310 315 320
Asn Ser Thr Phe Ile Ser Arg Asn Glu Leu Pro Ser Phe Ser Lys Ala
325 330 335
Val Asn Gln Arg Asp Ala Asp Leu Val Tyr Phe Trp Asn Lys Tyr Arg
340 345 350
Lys Ser Pro Val Gly Ser Ile Lys Lys Arg Asn Ala Gln Lys Glu Leu
355 360 365
Phe Asp Val Met Ala His Arg Leu His Leu Asp Asn Ser Ile Glu Leu
370 375 380
Ile Gly Lys Leu Leu Phe Gly Ser Glu Lys Gly Pro Glu Ile Leu Lys
385 390 395 400
Thr Val Arg Thr Thr Gly Leu Pro Leu Val Asp Asp Trp Asp Cys Leu
405 410 415
Lys Ala Met Val Arg Thr Phe Glu Thr Lys Cys Gly Ser Ile Ser Gln
420 425 430
Tyr Gly Met Lys His Met Arg Ser Met Ala Asn Ile Cys Asn Ala Gly
435 440 445
Ile Ser Lys Glu Val Met Ala Glu Ala Ser Ala Glu Ala Cys Thr Arg
450 455 460
Ile Pro Thr Thr Ser
465
<210> 83
<211> 489
<212> PRT
<213> Hordeum vulgare subsp. vulgare
<400> 83
Met Ala Met Ala Ser Phe His Leu Leu Pro Leu Ser Leu Leu Leu Leu
1 5 10 15
Leu Leu Val Val Ala Asn Ala Gly Thr Pro Pro Leu Glu Pro Gly Leu
20 25 30
Arg Leu Pro Ser Gln Arg Ala Ala Ala Gly Arg Gln Glu Asn Asp Gly
35 40 45
Ser Val Gly Thr Lys Trp Ala Val Leu Val Ala Gly Ser Asn Gly Tyr
50 55 60
Gln Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Ile Ile
65 70 75 80
Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp
85 90 95
Asp Ile Ala His Asn Pro Glu Asn Pro Arg Pro Gly Val Ile Ile Asn
100 105 110
His Pro Gln Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr
115 120 125
Gly Lys Glu Val Asn Ala Lys Asn Leu Phe Ala Val Leu Leu Gly Asn
130 135 140
Lys Thr Ala Val Ser Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro
145 150 155 160
Asn Asp His Ile Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val
165 170 175
Ile Gly Met Pro Thr Tyr Pro Tyr Ile Tyr Gly Asp Asp Leu Val Asp
180 185 190
Val Leu Lys Lys Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe
195 200 205
Tyr Leu Glu Ala Cys Glu Ala Gly Ser Val Phe Glu Gly Leu Leu Pro
210 215 220
Asn Asp Ile Gly Val Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser
225 230 235 240
Ser Trp Gly Ala Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu
245 250 255
Tyr Asp Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ser Trp Met Glu Asp
260 265 270
Ser Asp Val His Asn Leu Arg Thr Glu Ser Leu Lys Glu Gln Tyr Asn
275 280 285
Leu Val Lys Lys Arg Thr Ala Ala Gln Asp Ser Tyr Ser Tyr Gly Ser
290 295 300
His Val Met Gln Tyr Gly Ser Leu Asp Leu Asn Ala Gln His Leu Phe
305 310 315 320
Leu Tyr Ile Gly Ser Asn Pro Ala Asn Asp Asn Ala Thr Phe Val Glu
325 330 335
Glu Asn Ser Leu Pro Ser Phe Ser Arg Ala Val Asn Gln Arg Asp Ala
340 345 350
Asp Leu Val Tyr Phe Trp His Lys Tyr Arg Lys Leu Ala Glu Ser Ser
355 360 365
Pro Glu Lys Asn Asn Ala Arg Lys Gln Leu Leu Glu Met Met Gly His
370 375 380
Arg Ser His Val Asp Asn Ser Val Glu Leu Ile Gly Asn Leu Leu Phe
385 390 395 400
Gly Ser Ala Asp Gly Pro Met Val Leu Lys Ser Val Arg Pro Ala Gly
405 410 415
Glu Pro Leu Val Asp Asp Trp Asn Cys Leu Lys Ser Thr Val His Thr
420 425 430
Phe Glu Ser Gln Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met
435 440 445
Arg Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile Leu Pro Glu Thr Met
450 455 460
Val Lys Val Ala Ala Gln Ala Cys Thr Ser Ile Pro Thr Asn Pro Trp
465 470 475 480
Ser Gly Thr His Lys Gly Phe Ser Ala
485
<210> 84
<211> 485
<212> PRT
<213> Zea mays
<400> 84
Met Val Ala Asp Arg Leu Arg Leu Ala Leu Leu Leu Ser Ala Cys Leu
1 5 10 15
Cys Ser Ala Trp Ala Arg Pro Arg Leu Glu Pro Thr Ile Arg Leu Pro
20 25 30
Ser Glu Arg Ala Ala Ala Asp Glu Thr Asp Asp Asp Ala Val Gly Thr
35 40 45
Arg Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Tyr Asn Tyr Arg
50 55 60
His Gln Ala Asp Ile Cys His Ala Tyr Gln Ile Met Lys Lys Gly Gly
65 70 75 80
Leu Lys Asp Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala His
85 90 95
Ser Pro Glu Asn Pro Arg Pro Gly Val Ile Ile Asn His Pro Gln Gly
100 105 110
Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Arg Glu Val
115 120 125
Asn Val Asp Asn Phe Phe Ala Val Leu Leu Gly Asn Lys Thr Ala Leu
130 135 140
Arg Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile
145 150 155 160
Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro
165 170 175
Thr Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asp Val Leu Lys Lys
180 185 190
Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala
195 200 205
Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Asn Asp Ile Asn
210 215 220
Val Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr
225 230 235 240
Tyr Cys Pro Gly Glu Phe Pro Ser Pro Pro Pro Glu Tyr Asp Thr Cys
245 250 255
Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Phe His
260 265 270
Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Asn Leu Val Lys Asp
275 280 285
Arg Thr Ala Val Gln Asp Thr Phe Ser Tyr Gly Ser His Val Met Gln
290 295 300
Tyr Gly Ser Leu Glu Leu Asn Val Lys His Leu Phe Ser Tyr Ile Gly
305 310 315 320
Thr Asn Pro Ala Asn Asp Asp Asn Thr Ser Ile Glu Asp Asn Ser Leu
325 330 335
Pro Ser Phe Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val Tyr
340 345 350
Phe Trp Gln Lys Tyr Arg Lys Leu Ala Asp Ser Ser His Glu Lys Asn
355 360 365
Glu Ala Arg Arg Glu Leu Leu Glu Val Met Ala His Arg Ser His Val
370 375 380
Asp Ser Ser Val Glu Leu Ile Gly Ser Leu Leu Phe Gly Ser Glu Asp
385 390 395 400
Gly Pro Arg Val Leu Lys Ala Val Arg Ala Ala Gly Glu Pro Leu Val
405 410 415
Asp Asp Trp Ser Cys Leu Lys Ser Thr Val Arg Thr Phe Glu Ala Gln
420 425 430
Cys Gly Ser Leu Ala His Tyr Gly Met Lys His Met Arg Ser Phe Pro
435 440 445
Asn Ile Cys Asn Ala Gly Ile Leu Pro Glu Ala Val Ser Lys Val Ala
450 455 460
Ala Gln Ala Cys Thr Ser Ile Pro Ser Asn Pro Trp Ser Ser Ile His
465 470 475 480
Lys Gly Phe Ser Ala
485
<210> 85
<211> 487
<212> PRT
<213> Setaria italica
<400> 85
Met Met Ala Ala Ala Arg Leu Arg Leu Ala Leu Leu Leu Tyr Val Phe
1 5 10 15
Met Cys Ala Ala Trp Ala Arg Pro Gly Leu Glu Pro Ala Ile Arg Leu
20 25 30
Pro Ser Glu Arg Ala Ala Ala Ala Gly Glu Gly Thr Asp Asp Ala Val
35 40 45
Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Tyr Asn
50 55 60
Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln Ile Leu Lys Lys
65 70 75 80
Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile
85 90 95
Ala His Ser Pro Glu Asn Pro Arg Pro Gly Val Ile Ile Asn His Pro
100 105 110
Gln Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Arg
115 120 125
Glu Val Asn Val Asn Asn Phe Phe Ala Val Leu Leu Gly Asn Lys Thr
130 135 140
Ala Val Ser Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp
145 150 155 160
His Ile Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly
165 170 175
Met Pro Thr Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asn Val Leu
180 185 190
Lys Lys Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu
195 200 205
Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Asn Asp
210 215 220
Ile Asn Val Tyr Ala Thr Thr Ala Ser Asn Ala Asp Glu Ser Ser Trp
225 230 235 240
Gly Thr Tyr Cys Pro Gly Glu Ser Pro Ser Pro Pro Pro Glu Tyr Asp
245 250 255
Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp
260 265 270
Phe His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Asn Leu Val
275 280 285
Lys Asp Arg Thr Ser Val His Asn Thr Phe Thr Tyr Gly Ser His Val
290 295 300
Met Gln Tyr Gly Ser Leu Asn Leu Asn Val Gln His Leu Phe Ser Tyr
305 310 315 320
Ile Gly Thr Asn Pro Ala Asn Asp Asp Asn Lys Phe Val Glu Gly Asn
325 330 335
Ser Leu Pro Ser Phe Thr Arg Ala Val Asn Gln Arg Asp Ala Asp Leu
340 345 350
Val Tyr Phe Trp Gln Lys Tyr Arg Lys Val Ala Glu Gly Ser Pro Gly
355 360 365
Lys Asn Asp Ala Arg Lys Glu Leu Leu Glu Val Met Ala His Arg Ser
370 375 380
His Val Asp Asn Ser Val Glu Leu Ile Gly Ser Leu Leu Phe Gly Ser
385 390 395 400
Glu Asp Gly Pro Arg Val Leu Lys Ala Val Arg Ala Ala Gly Glu Pro
405 410 415
Leu Val Asp Asp Trp Ser Cys Leu Lys Ser Met Val Arg Ala Phe Glu
420 425 430
Ala Gln Cys Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg Ser
435 440 445
Phe Ala Asn Ile Cys Asn Ala Gly Ile Leu Pro Asp Ala Val Ser Lys
450 455 460
Val Ala Ala Gln Ala Cys Thr Ser Ile Pro Ser Asn Pro Trp Ser Ser
465 470 475 480
Ile His Met Gly Phe Ser Ala
485
<210> 86
<211> 460
<212> PRT
<213> Medicago truncatula
<400> 86
Met Asp Phe Ser Gln Phe Ser Thr Ile Leu Phe Leu Thr Val Ile Leu
1 5 10 15
Thr Ile Phe Ala Ala Val Ser Gly Ser Arg Asp Leu Pro Gly Asp Tyr
20 25 30
Ile Arg Leu Pro Ser Gln Ser Gln Ala Ser Arg Phe Phe His Glu Pro
35 40 45
Glu Asn Asp Asp Asn Asp Gln Gly Thr Arg Trp Ala Ile Leu Leu Ala
50 55 60
Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His
65 70 75 80
Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile Ile
85 90 95
Val Phe Met Tyr Asp Asp Ile Ala Ser Asn Val Glu Asn Pro Arg Pro
100 105 110
Gly Val Ile Ile Asn Lys Pro Asp Gly Gly Asp Val Tyr Glu Gly Val
115 120 125
Pro Lys Asp Tyr Thr Gly Ala Glu Val His Ala Asp Asn Phe Tyr Ala
130 135 140
Ala Leu Leu Gly Asn Lys Ser Ala Leu Thr Gly Gly Ser Gly Lys Val
145 150 155 160
Val Asp Ser Gly Pro Asn Asp His Ile Phe Val Tyr Tyr Thr Asp His
165 170 175
Gly Gly Pro Gly Val Leu Gly Met Pro Val Gly Pro Tyr Leu Tyr Ala
180 185 190
Ser Asp Leu Asn Glu Val Leu Lys Lys Lys His Ala Ser Gly Ser Tyr
195 200 205
Lys Ser Leu Val Phe Tyr Leu Glu Lys Ile Ser Ile Ser Met Arg Gln
210 215 220
Thr Ala Ser Asn Ala Val Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly
225 230 235 240
Glu Tyr Pro Pro Pro Pro Pro Glu Tyr Ser Thr Cys Leu Gly Asp Leu
245 250 255
Tyr Ser Ile Ala Trp Met Glu Asp Ser Asp Ile His Asn Leu Arg Thr
260 265 270
Glu Ser Leu His Gln Gln Tyr Lys Leu Val Lys Asp Arg Thr Ile Asn
275 280 285
Gly Tyr Tyr Gly Ser His Val Met Glu Tyr Gly Asp Val Gly Leu Ser
290 295 300
Asn Asn His Leu Phe Leu Tyr Leu Gly Thr Asn Pro Ala Asn Asp Asn
305 310 315 320
Ile Ser Phe Val Asp Glu Ser Ser Leu Lys Leu Arg Ser Pro Ser Thr
325 330 335
Ala Val Asn Gln Arg Asp Ala Asp Leu Ile His Phe Trp Asp Lys Phe
340 345 350
Arg Lys Ala Pro Glu Gly Ser Leu Arg Lys Asn Glu Ala Gln Lys Glu
355 360 365
Val Leu Glu Ala Met Ser His Arg Met His Val Asp Asn Ser Val Lys
370 375 380
Leu Ile Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly Thr Glu Leu Leu
385 390 395 400
Asp Asn Val Arg Pro Ala Gly Ser Pro Leu Val Asp Asn Trp Asp Cys
405 410 415
Leu Lys Thr Met Val Lys Thr Phe Glu Thr His Cys Gly Ser Leu Ser
420 425 430
Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn Ala
435 440 445
Gly Ile Gln Thr Glu Gln Met Ala Glu Ala Ser Ala
450 455 460
<210> 87
<211> 478
<212> PRT
<213> Populus trichocarpa
<400> 87
Met Thr Gly Leu Ala Thr Gly Ala Ile Phe Leu Leu Ile Ser Leu Cys
1 5 10 15
Gly Ile Ala Ala Ala Gly Arg Asp Thr Val Gly Asp Val Leu Arg Leu
20 25 30
Pro Ser Glu Ala Ser Arg Phe Phe His Asn Asp Asp Asn Ser Asp Asp
35 40 45
Asp Ser Thr Gly Thr Arg Trp Ala Ile Leu Leu Ala Gly Ser Asn Gly
50 55 60
Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Leu
65 70 75 80
Leu Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile Ile Val Phe Met Tyr
85 90 95
Asp Asp Ile Ala Tyr Asn Ser Glu Asn Pro Arg Arg Gly Val Ile Ile
100 105 110
Asn Ser Pro Gln Gly Glu Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr
115 120 125
Thr Gly Glu Asp Val Thr Val Gly Asn Phe Phe Ala Ala Ile Leu Gly
130 135 140
Asn Lys Thr Ala Leu Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly
145 150 155 160
Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His Gly Gly Pro Gly
165 170 175
Val Leu Gly Met Pro Thr Asn Pro Tyr Leu Tyr Ala Asp Asp Leu Ile
180 185 190
Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val
195 200 205
Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu
210 215 220
Pro Gln Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu
225 230 235 240
Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Asn Pro Ser Pro Pro Pro
245 250 255
Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu
260 265 270
Asp Ser Asp Ile His Asn Leu Gln Thr Glu Thr Leu His Gln Gln Tyr
275 280 285
Glu Leu Val Lys Arg Arg Thr Ser Asn Asp Asn Ser Pro Tyr Gly Ser
290 295 300
His Val Met Gln Tyr Gly Asp Val Gly Leu Ser Lys Asp Asn Ile Phe
305 310 315 320
Leu Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Phe Thr Phe Met Asp
325 330 335
Glu Asn Leu Leu Arg Pro Arg Ser Lys Ala Val Asn Gln Arg Asp Ala
340 345 350
Asp Leu Val His Phe Trp Asp Lys Tyr Arg Lys Ala Pro Glu Gly Ser
355 360 365
Ser Arg Lys Val Glu Ala Gln Lys Gln Phe Val Glu Ala Met Ser His
370 375 380
Arg Met His Ile Asp His Ser Ile Lys Leu Ile Gly Lys Leu Leu Phe
385 390 395 400
Gly Ile Glu Lys Ala Ser Glu Val Leu Asn Ala Ile Arg Pro Ala Gly
405 410 415
Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Thr Leu Val Lys Phe
420 425 430
Tyr Gly Ser Gln Pro Leu Leu Tyr His Arg Leu Thr Cys Leu Phe Ser
435 440 445
Leu Ile Ala Ser Pro Ala Gly Asp Thr Ser Lys Asp Ser Phe Pro Phe
450 455 460
Leu Arg Lys Cys Leu Leu Phe Phe Tyr Ala Gly Glu Asp Phe
465 470 475
<210> 88
<211> 431
<212> PRT
<213> Genlisea aurea
<400> 88
Asp Asp Asp Ile Val Gly Thr Arg Trp Ala Val Leu Val Ala Gly Ser
1 5 10 15
Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr
20 25 30
Gln Thr Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe
35 40 45
Met Tyr Asp Asp Ile Ala Tyr Asn Asp Glu Asn Pro Arg Pro Gly Val
50 55 60
Ile Ile Asn His Pro His Gly Glu Asn Val Tyr Asp Gly Val Pro Lys
65 70 75 80
Asp Tyr Val Gly Asp Asp Val Thr Val Asp Asn Phe Phe Ala Val Leu
85 90 95
Leu Gly Asn Lys Thr Ala Leu Ser Gly Gly Ser Gly Lys Val Val Asp
100 105 110
Ser Gly Pro Asn Asp His Ile Phe Val Tyr Tyr Ser Asp His Gly Gly
115 120 125
Pro Gly Val Leu Gly Met Pro Thr Asp Pro Tyr Leu Tyr Ala Asn Asp
130 135 140
Leu Ile Asp Val Leu Lys Arg Lys His Ala Ser Gly Thr Tyr Lys Ser
145 150 155 160
Leu Val Phe Tyr Val Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly
165 170 175
Leu Leu Pro Glu Gly Leu Asn Ile Phe Ala Thr Thr Ala Ser Asn Ala
180 185 190
Glu Glu Asn Ser Trp Gly Thr Tyr Cys Pro Gly Asp Asp Ile Gly Pro
195 200 205
Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Met Tyr Ser Val Ser Trp
210 215 220
Met Glu Asp Ser Asp Gln His Asn Leu Arg Thr Glu Thr Leu Arg Gln
225 230 235 240
Gln Tyr His Val Val Arg Glu Arg Thr Ala Arg Asp Asn Ser His Arg
245 250 255
Tyr Gly Ser His Val Met Gln Tyr Gly Asp Leu Lys Leu Ser Val Asp
260 265 270
Lys Leu Phe Leu Tyr Met Gly Ser Asn Pro Ser Asn Asp Asn Ser Thr
275 280 285
Phe Gly Gly Ser Val His Leu Ser Gly Asn Ser Ser Trp Pro Ser Ser
290 295 300
Met Ala Val Ser Gln Arg Asp Ala Asp Val Leu His Phe Trp Asp Lys
305 310 315 320
Phe Arg Lys Ala Pro Glu Gly Ser Ser Arg Lys Ala Glu Ala Gln Lys
325 330 335
Gln Leu Ala Glu Val Met Val Arg Arg Ser Arg Val Asp Ile Ser Val
340 345 350
Val Ser Ile Gly Lys Leu Leu Phe Gly Ser Ser Glu Ile Met Asn Ala
355 360 365
Ile Arg Pro Ser Gly Lys Ser Leu Val Asp Asp Trp Asp Cys Leu Lys
370 375 380
Ser Leu Val Arg Ile Phe Glu Thr Tyr Cys Ser Ser Leu Ser Gly Tyr
385 390 395 400
Gly Met Lys His Met Arg Ser Ile Ala Asn Met Cys Asn Ala Gly Val
405 410 415
Ser Glu Glu Gln Met Ser Glu Ala Ser Ser Gln Val Cys Ser Ser
420 425 430
<210> 89
<211> 422
<212> PRT
<213> Triticum urartu
<400> 89
Met Ser Arg Ala Asp Ile Cys His Ala Tyr Gln Ile Leu Lys Lys Gly
1 5 10 15
Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala
20 25 30
His Asn Leu Glu Asn Pro Arg Pro Gly Val Ile Ile Asn His Pro Gln
35 40 45
Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Lys Glu
50 55 60
Val Asn Val Lys Asn Leu Phe Ala Val Leu Leu Gly Asn Lys Thr Ala
65 70 75 80
Val Asn Gly Gly Ser Gly Lys Val Leu Asp Ser Gly Pro Asn Asp His
85 90 95
Ile Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val Ile Gly Met
100 105 110
Pro Thr Asn Pro Tyr Val Tyr Gly Asp Asp Leu Val Asp Val Leu Lys
115 120 125
Lys Lys His Ala Ala Gly Ser Tyr Lys Ser Leu Val Phe Tyr Leu Glu
130 135 140
Ala Cys Glu Ala Gly Ser Val Phe Glu Gly Leu Leu Pro Asn Asp Ile
145 150 155 160
Gly Val Tyr Ala Thr Thr Ala Ser Asp Ala Glu Glu Ser Ser Trp Gly
165 170 175
Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Asp Thr
180 185 190
Cys Leu Gly Asp Leu Tyr Ser Ile Ser Trp Met Glu Asp Ser Asp Val
195 200 205
His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Asp Leu Val Lys
210 215 220
Lys Arg Thr Ala Ala Gln Asp Ser Tyr Ser Tyr Gly Ser His Val Met
225 230 235 240
Gln Tyr Gly Ser Leu Asp Leu Asn Ala Gln Gln Leu Phe Leu Tyr Ile
245 250 255
Gly Ser Asn Pro Ala Asn Asn Asn Thr Thr Phe Val Glu Asp Asn Ser
260 265 270
Leu Pro Ser Phe Ser Arg Ala Val Asn Gln Arg Asp Ala Asp Leu Val
275 280 285
Tyr Phe Trp His Lys Tyr Arg Lys Leu Ala Glu Ser Ser Pro Glu Lys
290 295 300
Asn Asp Ala Arg Lys Gln Leu Leu Glu Met Thr Ser His Arg Ser His
305 310 315 320
Ile Asp Asn Ser Val Glu Leu Ile Gly Asn Leu Leu Phe Gly Phe Ala
325 330 335
Asp Gly Pro Met Val Leu Lys Thr Val Arg Pro Ala Gly Glu Pro Leu
340 345 350
Val Asp Asp Trp Ser Cys Leu Lys Ser Thr Val Arg Ala Phe Glu Ser
355 360 365
Gln Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg Ser Phe
370 375 380
Ala Asn Ile Cys Asn Ala Gly Val Leu Pro Glu Ala Met Val Lys Val
385 390 395 400
Ala Ala Gln Ala Cys Lys Ser Ile Pro Thr Asn Pro Trp Ser Ala Thr
405 410 415
His Lys Gly Phe Ser Ala
420
<210> 90
<211> 474
<212> PRT
<213> Hordeum vulgare subsp. vulgare
<400> 90
Met Ala Arg Leu Pro Cys Ser Pro Leu Leu Leu Leu Leu Val Leu Ser
1 5 10 15
Ser Gln Leu Ala Leu Leu Val Ala Gly Glu Phe Leu Arg Leu Pro Ser
20 25 30
Glu Lys Asp Val Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser
35 40 45
Asn Gly Tyr Tyr Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr
50 55 60
Gln Ile Met Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe
65 70 75 80
Met Tyr Asp Asp Ile Ala Asn Asn Arg Asp Asn Pro Arg Pro Gly Val
85 90 95
Ile Ile Asn His Pro Lys Gly Gly Asp Val Tyr Ala Gly Val Pro Lys
100 105 110
Asp Tyr Thr Gly Ala Asp Val Asn Thr Asn Asn Phe Leu Ala Ala Leu
115 120 125
Leu Gly Asp Lys Ser Lys Leu Thr Gly Ser Gly Ser Gly Lys Val Val
130 135 140
Ser Ser Gly Pro Asp Asp His Ile Phe Val Tyr Tyr Ala Asp His Gly
145 150 155 160
Gly Pro Gly Ile Leu Gly Met Pro Glu Asp Glu Glu Tyr Leu Tyr Ala
165 170 175
Asn Asp Leu Val Arg Thr Leu Glu Lys Lys His Ala Gly Gly Ala Gly
180 185 190
Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile
195 200 205
Phe Glu Gly Leu Leu Pro Gly Asn Ile Ser Val Tyr Ala Thr Thr Ala
210 215 220
Ala Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Asp Asp
225 230 235 240
Glu Gly Ala Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly Asp Leu Tyr
245 250 255
Ser Val Ala Trp Met Glu Asp Ser Asp Ala His Asn Leu Asn Ala Glu
260 265 270
Ser Leu Lys Gln Gln Tyr Glu Arg Val Arg Asn Arg Thr Ser Ala Asp
275 280 285
Gly Thr Tyr Ser Leu Gly Ser His Val Met Gln Tyr Gly Asp Leu Gly
290 295 300
Leu Asn Asp Gln Ser Leu Phe Gln Tyr Ile Gly Thr Asn Pro Ala Asn
305 310 315 320
Asp Asn Ala Thr Phe Val Gln Ser Ser Ser Ser Ser Arg Gln Leu Pro
325 330 335
Gly Ala Arg Val Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp His
340 345 350
Lys Tyr Arg Arg Ser Ala Glu Gly Ser Ala Glu Lys Val Glu Ala Arg
355 360 365
Arg Arg Leu Val Glu Thr Met Ala Arg Arg Ser Arg Val Asp Ser Ser
370 375 380
Val Glu Leu Ile Gly Gly Leu Leu Phe Gly Ser Glu Glu Gly Ala Lys
385 390 395 400
Val Leu Gly Thr Val Arg Pro Ala Gly Gln Pro Val Val Asp Asp Trp
405 410 415
Gly Cys Leu Lys Ser Val Val Arg Arg Phe Glu Glu Arg Cys Gly Pro
420 425 430
Leu Thr Gln Tyr Gly Met Lys His Met Arg Ser Leu Ala Asn Ile Cys
435 440 445
Asn Ala Gly Val Arg Glu Glu Val Met Asp Lys Ala Ala Ser Gln Ala
450 455 460
Cys Ala Ala Ser Pro Ser Ser Leu Ile Ile
465 470
<210> 91
<211> 424
<212> PRT
<213> Aegilops tauschii
<400> 91
Met Ser Gln Val Arg Ala Asp Ile Cys His Ala Tyr Gln Ile Leu Lys
1 5 10 15
Thr Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp
20 25 30
Ile Ala His Asn Leu Glu Asn Pro Arg Pro Gly Val Ile Ile Asn His
35 40 45
Pro Gln Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly
50 55 60
Lys Glu Val Asn Val Lys Asn Leu Phe Ala Val Leu Leu Gly Asn Lys
65 70 75 80
Thr Ala Val Ser Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn
85 90 95
Asp His Ile Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val Ile
100 105 110
Gly Met Pro Thr Tyr Pro Tyr Val Tyr Gly Asp Asp Leu Val Asp Val
115 120 125
Leu Lys Lys Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr
130 135 140
Leu Glu Ala Cys Glu Ala Gly Ser Val Phe Glu Gly Leu Leu Pro Asn
145 150 155 160
Asp Ile Gly Val Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser Ser
165 170 175
Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Ser Glu Tyr
180 185 190
Asp Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ser Trp Met Glu Asp Ser
195 200 205
Asp Val His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Asp Leu
210 215 220
Val Lys Lys Arg Thr Ala Ala Gln Asp Ser Tyr Ser Tyr Gly Ser His
225 230 235 240
Val Met Gln Tyr Gly Ser Leu Asp Leu Asn Asp Gln His Leu Phe Leu
245 250 255
Tyr Ile Gly Ser Asn Pro Ala Asn Asp Asn Thr Thr Phe Val Glu Asp
260 265 270
Asn Ser Leu Pro Ser Phe Ser Arg Ala Val Asn Gln Arg Asp Ala Asp
275 280 285
Leu Val Tyr Phe Trp Arg Lys Tyr Gln Lys Leu Ala Glu Ser Ser Thr
290 295 300
Glu Lys Asn Asp Ala Arg Lys Gln Leu Leu Glu Met Met Gly His Arg
305 310 315 320
Ser His Ile Asp Asn Ser Val Glu Leu Ile Gly Asn Leu Leu Phe Gly
325 330 335
Phe Ala Asp Gly Pro Met Val Leu Lys Thr Val Arg Pro Ala Gly Glu
340 345 350
Pro Leu Ala Asp Asp Trp Ser Cys Leu Lys Ser Met Val Arg Ala Phe
355 360 365
Glu Ser Gln Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg
370 375 380
Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile Leu Pro Glu Ala Met Val
385 390 395 400
Lys Met Ala Ala Gln Ala Cys Thr Ser Ile Pro Thr Asn Pro Trp Ser
405 410 415
Ala Thr His Asn Gly Phe Ser Ala
420
<210> 92
<211> 461
<212> PRT
<213> Solanum tuberosum
<400> 92
Met Asn Arg Ser Ile Ala Gly Val Leu Phe Leu Ile Ala Leu Ser Leu
1 5 10 15
Asn Val Ser Val Ser Glu Ser Arg Asn Phe Leu Lys Leu Pro Ser Glu
20 25 30
Gly Ser Arg Phe Phe Asp Ala Asp Glu Ser Asp Ser Val Gly Thr Arg
35 40 45
Trp Ala Ile Leu Leu Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His
50 55 60
Gln Ala Asp Ile Cys His Ala Tyr Gln Leu Leu Lys Lys Gly Gly Leu
65 70 75 80
Lys Asp Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Asn Asn
85 90 95
Glu Glu Asn Pro Arg Gln Gly Val Ile Ile Asn Ser Pro His Gly Glu
100 105 110
Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Thr Gly Asp Asp Val Thr
115 120 125
Val Asn Asn Phe Leu Ala Ala Leu Leu Gly Asn Lys Thr Ala Leu Thr
130 135 140
Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile Phe
145 150 155 160
Ile Phe Cys Ser Asp His Gly Gly Ala Gly Val Ile Gly Met Pro Thr
165 170 175
Asp Pro Tyr Leu Tyr Ala Asn Asp Leu Ile Asp Ala Leu Lys Lys Lys
180 185 190
His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys
195 200 205
Glu Ser Gly Ser Met Ser Glu Gly Leu Leu Pro Glu Gly Leu Asn Val
210 215 220
Tyr Ala Thr Thr Ala Ser Asn Ala Asp Glu Ser Ser Trp Gly Thr Tyr
225 230 235 240
Cys Pro Gly Glu Tyr Pro Ser Pro Pro Ile Glu Tyr Gly Thr Cys Leu
245 250 255
Gly Asp Leu Tyr Ser Ile Ser Trp Met Glu Asp Ser Asp Arg His Asn
260 265 270
Leu Arg Thr Glu Thr Leu Lys Gln Gln Tyr His Leu Val Lys Glu Arg
275 280 285
Thr Ala Ser Gly Asn Pro Ala Tyr Gly Ser His Val Met Gln Tyr Gly
290 295 300
Asp Val His Leu Ser Lys Asp Ala Leu Phe Leu Tyr Met Gly Thr Asp
305 310 315 320
Pro Ala Asn Asp Asn Tyr Thr Phe Val Asp Asp Asn Ser Leu Arg Val
325 330 335
Ser Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val His Phe Trp Tyr
340 345 350
Lys Phe His Lys Ala Pro Glu Gly Ser Val Arg Lys Thr Glu Ala Gln
355 360 365
Lys Gln Leu Asn Glu Ala Ile Ser His Arg Met His Leu Asp Asn Ser
370 375 380
Ile Ala Leu Val Gly Lys Leu Leu Phe Gly Ile Lys Lys Gly Pro Glu
385 390 395 400
Val Leu Thr Ser Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp
405 410 415
Asp Cys Leu Lys Ser Tyr Val Ser Thr Pro Thr Pro Phe Ser Ser Ile
420 425 430
Ser Phe Glu Phe Ser Gln Ile Tyr Ser Cys Ile Ser Leu Tyr Gly Gly
435 440 445
Met Arg Leu Arg Ser Leu Val Leu Asp Ile Leu Leu Val
450 455 460
<210> 93
<211> 441
<212> PRT
<213> Triticum urartu
<400> 93
Met Ala Met Ala Ser Phe Arg Pro Pro Pro Leu Ala Leu Leu Leu Ala
1 5 10 15
Ala Cys Leu Ser Ala Leu Ala Asp Ile Cys His Ala Tyr Gln Ile Met
20 25 30
Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp
35 40 45
Asp Ile Ala His Asn Pro Glu Asn Pro Arg Pro Gly Val Ile Ile Asn
50 55 60
His Pro Gln Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr
65 70 75 80
Gly Lys Glu Val Asn Val Lys Asn Phe Phe Ala Val Leu Leu Gly Asn
85 90 95
Lys Thr Ala Val Ser Gly Gly Asn Gly Lys Val Val Asp Ser Gly Pro
100 105 110
Asn Asp His Ile Phe Val Phe Tyr Ser Asp His Gly Gly Pro Gly Val
115 120 125
Leu Gly Met Pro Thr Tyr Pro Tyr Leu Tyr Gly Asp Asp Leu Val Asp
130 135 140
Val Leu Lys Lys Lys His Ala Ala Gly Thr Tyr Lys Ser Leu Val Phe
145 150 155 160
Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro
165 170 175
Asn Asp Ile Gly Val Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser
180 185 190
Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu
195 200 205
Tyr Asp Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ser Trp Met Glu Asp
210 215 220
Ser Asp Val His Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Asn
225 230 235 240
Leu Val Lys Lys Arg Thr Ala Ala Gln Asp Ser Tyr Ser Tyr Gly Ser
245 250 255
His Val Met Gln Tyr Gly Ser Leu Asp Leu Asn Ala Glu His Leu Phe
260 265 270
Ser Tyr Ile Gly Ser Asn Pro Ala Asn Glu Asn Thr Thr Phe Val Glu
275 280 285
Asp Asn Ala Leu Pro Ser Phe Ser Arg Ala Val Asn Gln Arg Asp Ala
290 295 300
Asp Leu Val Tyr Phe Trp Gln Lys Tyr Arg Lys Leu Ala Glu Ser Ser
305 310 315 320
Pro Glu Lys Asn Asp Ala Arg Lys Gln Leu Leu Glu Met Met Gly His
325 330 335
Arg Ser His Ile Asp Asn Ser Ile Glu Leu Ile Gly Asn Leu Leu Phe
340 345 350
Gly Ser Ala Gly Gly Pro Met Val Leu Lys Ala Val Arg Pro Ala Gly
355 360 365
Glu Pro Leu Val Asp Asp Trp Ser Cys Leu Lys Ser Thr Val Arg Thr
370 375 380
Phe Glu Ser Gln Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met
385 390 395 400
Arg Ser Phe Ala Asn Ile Cys Asn Ala Gly Ile Val Pro Glu Ala Thr
405 410 415
Ala Lys Val Ala Ala Gln Ala Cys Thr Ser Ile Pro Thr Asn Pro Trp
420 425 430
Ser Ala Thr His Lys Gly Phe Ser Ala
435 440
<210> 94
<211> 394
<212> PRT
<213> Citrus clementina
<400> 94
Met Tyr Asp Asp Ile Ala Phe Asn Glu Glu Asn Pro Arg Pro Gly Val
1 5 10 15
Ile Ile Asn His Pro His Gly Asp Asp Val Tyr Lys Gly Val Pro Lys
20 25 30
Asp Tyr Thr Gly Glu Asp Val Thr Val Glu Asn Phe Phe Ala Val Ile
35 40 45
Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser Gly Lys Val Val Asp
50 55 60
Ser Gly Pro Asn Asp His Ile Phe Ile Phe Tyr Ser Asp His Gly Gly
65 70 75 80
Pro Gly Val Leu Gly Met Pro Thr Ser Arg Tyr Ile Tyr Ala Asp Glu
85 90 95
Leu Ile Asp Val Leu Lys Lys Lys His Ala Ser Gly Asn Tyr Lys Ser
100 105 110
Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly
115 120 125
Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala
130 135 140
Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Ile Pro Gly Pro
145 150 155 160
Pro Pro Glu Tyr Ser Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ala Trp
165 170 175
Met Glu Asp Ser Asp Ile His Asn Leu Arg Thr Glu Thr Leu His Gln
180 185 190
Gln Tyr Glu Leu Val Lys Thr Arg Thr Ala Ser Tyr Asn Ser Tyr Gly
195 200 205
Ser His Val Met Gln Tyr Gly Asp Ile Gly Leu Ser Lys Asn Asn Leu
210 215 220
Phe Thr Tyr Leu Gly Thr Asn Pro Ala Asn Asp Asn Tyr Thr Phe Val
225 230 235 240
Asp Glu Asn Ser Leu Arg Pro Ala Ser Lys Ala Val Asn Gln Arg Asp
245 250 255
Ala Asp Leu Leu His Phe Trp Asp Lys Tyr Arg Lys Ala Pro Glu Gly
260 265 270
Thr Pro Arg Lys Ala Glu Ala Gln Lys Gln Phe Phe Glu Ala Met Ser
275 280 285
His Arg Met His Val Asp His Ser Ile Lys Leu Ile Gly Lys Leu Leu
290 295 300
Phe Gly Ile Glu Lys Gly Pro Glu Ile Leu Asn Thr Val Arg Pro Ala
305 310 315 320
Gly Gln Pro Leu Val Asp Asp Trp Gly Cys Leu Lys Ser Leu Val Arg
325 330 335
Thr Phe Glu Ser His Cys Gly Ala Leu Ser Gln Tyr Gly Met Lys His
340 345 350
Met Arg Ser Leu Ala Asn Ile Cys Asn Thr Gly Ile Gly Lys Glu Lys
355 360 365
Met Ala Glu Ala Ser Ala Gln Ala Cys Glu Asn Ile Pro Ser Gly Pro
370 375 380
Trp Ser Ser Leu Asp Lys Gly Phe Ser Ala
385 390
<210> 95
<211> 473
<212> PRT
<213> Setaria italica
<400> 95
Met Ala Thr Ala Thr Thr Arg Leu Arg Cys Leu Leu Leu Leu Phe Leu
1 5 10 15
Val Gln Leu Leu Leu Leu Leu Ser Ala Ala Ala Gly Ala Arg Trp Gln
20 25 30
Asp Phe Leu Arg Leu Pro Ser Glu Gly Gly Asp Ala Ala Ala Gly Thr
35 40 45
Arg Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Tyr Asn Tyr Arg
50 55 60
His Gln Ala Asp Val Cys His Ala Tyr Gln Ile Met Lys Lys Gly Gly
65 70 75 80
Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Ser
85 90 95
Ser Pro Asp Asn Pro Arg Pro Gly Val Ile Ile Asn His Pro Ser Gly
100 105 110
Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Glu Asp Val
115 120 125
Thr Val Asn Asn Phe Leu Ala Val Leu Leu Gly Asn Arg Ser Ala Val
130 135 140
Ser Gly Gly Ser Gly Lys Val Val Ala Ser Gly Pro Gly Asp His Val
145 150 155 160
Phe Val Tyr Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro
165 170 175
Ser Gly Asp Tyr Leu Tyr Ala Lys Asp Leu Val Gly Ala Leu Glu Arg
180 185 190
Lys His Asp Ala Gly Gly Tyr Arg Ser Leu Val Phe Tyr Leu Glu Ala
195 200 205
Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly Ile Asn
210 215 220
Val Tyr Ala Thr Thr Ala Ala Asn Ala Glu Glu Ser Ser Trp Gly Thr
225 230 235 240
Tyr Cys Pro Gly Asp Asp Gln Gly Pro Pro Pro Glu Phe Asp Thr Cys
245 250 255
Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Val His
260 265 270
Asn Leu Arg Thr Glu Ser Leu Lys Gln Gln Tyr Glu Val Val Lys Asp
275 280 285
Arg Thr Ser Ala His Gly Thr Tyr Ser Leu Gly Ser His Val Met Gln
290 295 300
Tyr Gly Asp Gln Ser Leu Asn Gly Gln Ser Leu Tyr Gln Phe Ile Gly
305 310 315 320
Thr Asp Pro Ala Asn Asp Asn Ala Thr Phe Gly Arg Asp Asn Ser Leu
325 330 335
Arg Arg Arg Ser Ser Gly Thr Val Asn Gln Arg Asp Ala Asp Leu Val
340 345 350
Tyr Phe Trp Gln Lys Tyr Lys Lys Ser Ala Glu Gly Thr Pro Glu Lys
355 360 365
Ala Glu Ala Arg Lys Arg Leu Leu Gln Val Met Ser Arg Arg Ser Arg
370 375 380
Val Asp Ser Ser Met Glu Leu Ile Gly Ser Leu Leu Phe Gly Ser Asp
385 390 395 400
Glu Gly Pro Lys Val Leu Gly Ala Val Arg Pro Ala Gly Gln Pro Leu
405 410 415
Ala Asp Asp Trp Asp Cys Leu Lys Ala Met Val His Ala Tyr Glu Ala
420 425 430
Gln Cys Gly Pro Leu Lys Gln Tyr Gly Met Lys His Met Arg Ser Phe
435 440 445
Ala Asn Ile Cys Asn Ala Gly Val Gly Glu Asp Ala Met Ala Lys Val
450 455 460
Ala Ser Gln Ala Cys Ala Ala Ala Arg
465 470
<210> 96
<211> 543
<212> PRT
<213> Aegilops tauschii
<400> 96
Met Ala Ser Asn Arg Leu Leu Pro Leu Ala Leu Leu Leu Leu Leu Leu
1 5 10 15
Ala Met Ala His Ala Asp Thr Pro Arg Leu Glu Pro Thr Val Arg Leu
20 25 30
Pro Ser Gln Arg Met Ala Ala Gly Gln Gly Asp Asp Gly Ser Val Gly
35 40 45
Thr Arg Trp Ala Ala Leu Val Ala Gly Ser Asn Gly Tyr Gln Asn Tyr
50 55 60
Arg His Gln Gly Arg Thr Leu Tyr His Gly Phe Tyr Ser Leu Leu Val
65 70 75 80
Gly Val Ser Ser Arg Asp Pro Pro Thr Phe Phe Phe Leu Ser Val Lys
85 90 95
Ser Ile Pro Ser Trp Glu Ile Arg Trp Val Asp Asp Asn Glu Val Leu
100 105 110
Cys Leu Ile Cys Gly Ala Lys Leu Cys Thr Val Gln Ala Asp Ile Cys
115 120 125
His Ala Tyr Gln Ile Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile
130 135 140
Ile Val Phe Met Tyr Asp Asp Ile Ala His Asn Leu Glu Asn Pro Arg
145 150 155 160
Pro Gly Val Ile Ile Asn His Pro Gln Gly Gly Asp Val Tyr Ser Gly
165 170 175
Val Pro Met Asp Tyr Thr Gly Lys Glu Val Asn Val Lys Asn Leu Phe
180 185 190
Ala Val Leu Leu Gly Asn Lys Thr Ala Val Ser Gly Gly Ser Gly Lys
195 200 205
Val Leu Asp Ser Gly Pro Asn Asp His Ile Phe Val Phe Tyr Ser Asp
210 215 220
His Gly Gly Pro Gly Val Ile Gly Met Pro Thr Asn Pro Tyr Val Tyr
225 230 235 240
Gly Asp Asp Leu Val Asp Val Leu Lys Lys Lys His Ala Ala Gly Thr
245 250 255
Tyr Arg Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ala Gly Ser Val
260 265 270
Phe Glu Gly Leu Leu Pro Asn Asp Ile Ser Val Tyr Thr Thr Thr Ala
275 280 285
Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr
290 295 300
Pro Ser Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly Asp Leu Tyr Ser
305 310 315 320
Ile Ser Trp Met Glu Asp Ser Asp Val His Asn Leu Arg Thr Glu Ser
325 330 335
Leu Lys Gln Gln Tyr Asp Leu Val Lys Lys Arg Thr Ala Ala Gln Asp
340 345 350
Ser Tyr Asn Tyr Gly Ser His Val Met Gln Tyr Gly Ser Leu Asp Leu
355 360 365
Asn Ala Gln Gln Leu Phe Leu Tyr Ile Gly Ser Asn Pro Ala Asn Asn
370 375 380
Lys Thr Thr Phe Val Glu Asp Asn Ser Leu Pro Ser Phe Ser Arg Val
385 390 395 400
Val Asn Gln Arg Asp Ala Asp Leu Val Tyr Phe Trp His Lys Tyr Arg
405 410 415
Lys Leu Ala Glu Ser Ser Pro Glu Lys Asn Asp Ala Arg Lys Gln Leu
420 425 430
Leu Glu Met Met Ser His Arg Ser His Ile Asp Asn Ser Val Glu Leu
435 440 445
Ile Gly Asn Leu Leu Phe Gly Ser Ala Asp Gly Pro Met Val Leu Lys
450 455 460
Thr Val Arg Pro Ala Gly Glu Pro Leu Val Asp Asp Trp Ser Cys Leu
465 470 475 480
Lys Ser Thr Val Arg Ala Phe Glu Ser Gln Cys Gly Ser Leu Ala Gln
485 490 495
Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Ile Cys Asn Ala Gly
500 505 510
Val Leu Pro Glu Ala Thr Val Lys Val Ala Ala Gln Ala Cys Lys Ser
515 520 525
Ile Pro Thr Asn Pro Trp Ser Ala Thr His Lys Gly Phe Ser Ala
530 535 540
<210> 97
<211> 493
<212> PRT
<213> Picea sitchensis
<400> 97
Met Gly Ser Ser Lys Gly Phe Gln Leu Gly Leu Leu Cys Tyr Phe Leu
1 5 10 15
Leu Leu Ser Leu Asp Ser Ser Lys Val Ala Asp Gly Ala Arg Arg Asp
20 25 30
Trp Asn Ser Leu Leu Lys Leu Pro Thr Asn His Val Asp Ala Asp Ser
35 40 45
Asp Arg Ile Gly Thr Glu Trp Ala Val Leu Leu Ala Gly Ser Ser Gly
50 55 60
Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Ile
65 70 75 80
Leu Arg Arg Gly Gly Leu Lys Glu Glu Asn Ile Val Val Phe Met Tyr
85 90 95
Asp Asp Ile Ala Tyr Asp Glu Glu Asn Pro His Pro Gly Thr Ile Ile
100 105 110
Asn His Pro Gln Gly Ser Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr
115 120 125
Thr Gly Glu Asp Val Thr Val Asn Asn Phe Phe Ala Ala Ile Leu Gly
130 135 140
Asn Lys Ser Leu Val Thr Gly Gly Ser Gly Lys Val Val Glu Ser Gly
145 150 155 160
Pro Asn Asp Arg Ile Phe Ile Tyr Tyr Ser Asp His Gly Gly Pro Gly
165 170 175
Val Leu Gly Met Pro Leu Pro Pro Tyr Leu Tyr Ala Asn Asp Phe Val
180 185 190
Gln Val Leu Lys Lys Lys His Asp Ala Gly Ser Tyr Arg Glu Met Val
195 200 205
Ile Tyr Val Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu
210 215 220
Pro Thr Asp Leu Asn Ile Tyr Val Thr Thr Ala Ser Asn Ala Glu Glu
225 230 235 240
Asn Ser Trp Gly Thr Tyr Cys Pro Gly Met Asp Pro Pro Pro Pro Pro
245 250 255
Glu Tyr Asp Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu
260 265 270
Asp Ser Glu Ile Asn Asn Leu Lys Glu Glu Thr Leu Leu Gln Gln Tyr
275 280 285
Asp Leu Val Lys Leu Arg Thr Ser Asn His Asn Thr Tyr Met Ser Gly
290 295 300
Ser His Val Met Gln Tyr Gly Asn Ile Thr Ile Ser Gln Glu Glu Leu
305 310 315 320
Tyr Leu Tyr Met Gly Phe Asp Ser Ala Asn Ser Asn Ala Ser Leu Val
325 330 335
Leu Glu Asn Ser Pro Leu Leu Glu Lys Thr Glu Ala Lys Ala Ile Asn
340 345 350
Gln Arg Asp Ala Asp Leu Leu Tyr Met Trp Gln Lys Tyr Lys Lys Ser
355 360 365
Lys Glu Asp Ser Pro Glu Arg Leu Thr Ala Gln Thr Gln Leu Leu Glu
370 375 380
Phe Met Ala His Arg Met His Val Asp Lys Ser Val Lys Leu Val Gly
385 390 395 400
Asn Leu Leu Phe Gly Pro Glu Lys Gly Pro Ala Val Phe Asn Ala Val
405 410 415
Arg Pro Gln Gly Glu Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Lys
420 425 430
Met Val Arg Thr Phe Glu Gly His Cys Gly Ser Leu Ala Gln Tyr Gly
435 440 445
Met Lys His Met Arg Ala Leu Ala Asn Ile Cys Asn Glu Gly Ile Ser
450 455 460
Met Asp Thr Met Ala Thr Val Ser Ala Glu Ala Cys Thr Gln Phe Pro
465 470 475 480
Ala Gly Ser Trp Ser Ser Leu Gln Arg Gly Phe Ser Ala
485 490
<210> 98
<211> 380
<212> PRT
<213> Vicia narbonensis
<400> 98
Asp Val Cys His Ala Tyr Gln Leu Leu Arg Lys Gly Gly Leu Lys Glu
1 5 10 15
Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Tyr Ser Glu Glu
20 25 30
Asn Pro Arg Pro Gly Val Ile Ile Asn Ser Pro His Gly Glu Asn Val
35 40 45
Tyr Glu Gly Val Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val Gly
50 55 60
Asn Phe Phe Ala Ala Leu Leu Gly Asn Lys Ser Ala Leu Ser Gly Gly
65 70 75 80
Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp Arg Ile Phe Val Phe
85 90 95
Tyr Ser Asp His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Pro
100 105 110
Tyr Met Tyr Ala Ser Asp Leu Val Glu Val Leu Lys Ile Lys His Ala
115 120 125
Ala Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser
130 135 140
Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala
145 150 155 160
Thr Thr Ala Ala Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro
165 170 175
Gly Glu Asn Pro Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Ala Asp
180 185 190
Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Ile His Asn Leu Gln
195 200 205
Thr Glu Thr Leu His Gln Gln Tyr Glu Leu Val Lys Glu Arg Thr Ser
210 215 220
Asn Gly Asn Ser Asn Tyr Gly Ser His Val Met Gln Tyr Gly Asp Ile
225 230 235 240
Glu Leu Ser Lys Asp Ser Leu Phe Leu Tyr Leu Gly Ser Asn Pro Ser
245 250 255
Asn Glu Asn Phe Thr Phe Val Gly Arg Asn Ser Leu Val Pro Pro Ser
260 265 270
Lys Ala Ile Asn Gln Arg Asp Ala Asp Leu Ile His Phe Trp Asp Lys
275 280 285
Phe Arg Lys Ala Pro Gln Gly Ser Pro Arg Lys Ala Ala Ala Gln Lys
290 295 300
Glu Val Leu Glu Ala Met Ser His Arg Met His Ile Asp Asp Ser Ile
305 310 315 320
Lys Leu Val Gly Lys Leu Leu Phe Gly Met Lys Lys Gly Pro Glu Val
325 330 335
Leu Thr Ser Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Asp
340 345 350
Cys Leu Lys Thr Leu Val Arg Thr Phe Glu Thr Tyr Cys Gly Ser Leu
355 360 365
Ser Gln Tyr Gly Met Lys His Met Arg Ser Phe Ala
370 375 380
<210> 99
<211> 455
<212> PRT
<213> Nicotiana tabacum
<400> 99
Met Ile Arg Lys Asn Gly Val Val Pro Phe Leu Val Ala Leu Phe Val
1 5 10 15
Leu Val Cys Thr Ala Glu Gly Arg Asn Leu Leu Glu Ser Ile Val Glu
20 25 30
Asp Asp Asn Pro Thr Gly Thr Lys Trp Ala Val Leu Val Ala Gly Ser
35 40 45
Asn Glu Trp Asp Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr
50 55 60
Gln Leu Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe
65 70 75 80
Met Tyr Asp Asp Ile Ala Tyr Asn Lys Asn Asn Pro Arg Pro Gly Ile
85 90 95
Ile Ile Asn Ser Pro His Gly His Asp Val Tyr Lys Gly Val Pro Lys
100 105 110
Asp Tyr Thr Gly Lys Asp Cys Asn Ala Asp Asn Phe Phe Ala Val Ile
115 120 125
Leu Gly Asn Lys Ser Ala Leu Thr Gly Gly Ser Gly Lys Val Val Glu
130 135 140
Asn Gly Pro Asn Asp Tyr Ile Phe Ile Tyr Tyr Ala Asp His Gly Ala
145 150 155 160
Pro Gly Leu Ile Gly Met Pro Ser Gly Asp Val Val Tyr Ala Asp Asp
165 170 175
Leu Asn Arg Val Leu Ile Lys Lys His Thr Phe Gly Thr Tyr Ser Lys
180 185 190
Leu Val Phe Tyr Met Glu Ala Cys Glu Ser Gly Ser Met Phe Asp Gly
195 200 205
Leu Leu Pro Lys Gly Leu Asn Ile Tyr Val Thr Ala Ala Ser Lys Pro
210 215 220
Asp Glu Ser Ser Trp Ala Thr Tyr Cys Ile Arg Leu Gly Asp Glu Asp
225 230 235 240
Gln Cys Leu Gly Asp Leu Tyr Ser Val Ser Trp Leu Glu Asp Ser Asp
245 250 255
Leu His Asp Arg Gln Val Glu Thr Leu Glu Lys Gln Tyr Gln Leu Val
260 265 270
Arg Lys Arg Thr Leu Asn Asn Gly Thr Glu Glu Gly Ser His Val Met
275 280 285
Gln Tyr Gly Asp Leu His Ile Ser Glu Asp Pro Leu Phe Arg Tyr Met
290 295 300
Gly Ser Asn Ser Ala Lys Asn Ser Tyr Asn Thr Ser Asn Asn Asp Glu
305 310 315 320
Ser Trp Leu Pro Ser Arg Thr Val Asn Gln Arg Asp Val His Leu Met
325 330 335
His Leu Trp Ser Lys Phe Arg Ser Ala Pro Glu Gly Ser Ala Arg Lys
340 345 350
Ala Glu Ala His Arg Gln Leu Ser Glu Ala Leu Ser Gln Arg Glu Asp
355 360 365
Val Asp Asn Ser Val Arg His Ile Gly Glu Val Leu Phe Gly Val Glu
370 375 380
Lys Ser His Lys Leu Leu Asn Thr Val Arg Pro Ala Gly Gln Pro Leu
385 390 395 400
Val Asp Asp Trp Asp Cys Leu Lys Ser Phe Val Lys Ile Phe Glu Ser
405 410 415
Gln Cys Gly Thr Leu Thr Pro Tyr Gly Arg Lys His Val Arg Gly Phe
420 425 430
Ala Asn Leu Cys Asn Ala Gly Ile Arg Arg Glu Gln Met Ala Ala Ala
435 440 445
Ala Lys Gln Ala Cys Pro Pro
450 455
<210> 100
<211> 492
<212> PRT
<213> Aegilops tauschii
<400> 100
Met Ala Arg Leu Ser Cys Ser Pro Leu Leu Leu Leu Leu Phe Leu Ser
1 5 10 15
Ser Gln Leu Ala Leu Leu Val Ala Gly Glu Phe Leu Arg Leu Pro Ser
20 25 30
Glu Lys Asp Val Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser
35 40 45
Asn Gly Tyr Tyr Asn Tyr Arg His Gln Ala Gly Thr Lys Phe Pro Ile
50 55 60
Lys Tyr Ser Ser Leu Ile Thr Leu Met Glu Asn Ala Asp Val Cys His
65 70 75 80
Ala Tyr Gln Ile Met Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile
85 90 95
Val Phe Met Tyr Asp Asp Ile Ala Gly Asn Arg Asp Asn Pro Arg Pro
100 105 110
Gly Val Ile Ile Asn His Pro Lys Gly Gly Asp Val Tyr Ala Gly Val
115 120 125
Pro Lys Asp Tyr Thr Gly Ala Asp Val Asn Ala Asn Asn Phe Leu Ala
130 135 140
Ala Leu Leu Gly Asp Lys Ser Lys Leu Thr Gly Ser Gly Ser Gly Lys
145 150 155 160
Val Val Ser Ser Gly Ser Asp Asp His Ile Phe Val Tyr Tyr Ala Asp
165 170 175
His Gly Gly Pro Gly Ile Leu Gly Met Pro Gly Asp Glu Glu Tyr Leu
180 185 190
Tyr Ala Asn Asp Leu Val Arg Thr Leu Glu Lys Lys His Ala Gly Gly
195 200 205
Ala Gly Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly
210 215 220
Ser Ile Phe Glu Gly Leu Leu Pro Gly Asn Ile Gly Val Tyr Ala Thr
225 230 235 240
Thr Ala Ala Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly
245 250 255
Asp Asp Glu Gly Ala Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly Asp
260 265 270
Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp Ala His Asn Leu Asn
275 280 285
Ala Glu Ser Leu Lys Gln Gln Tyr Glu Arg Val Arg Asp Arg Thr Ser
290 295 300
Ala Ala Gly Thr Tyr Ser Leu Gly Ser His Val Met Gln Tyr Gly Asp
305 310 315 320
Leu Asp Leu Asn Asp Gln Ser Leu Phe Leu Tyr Ile Gly Thr Asn Pro
325 330 335
Ala Asn Asp Asn Ala Ser Phe Val Gln Gly Ser Ser Ser Thr Ser Arg
340 345 350
Gln Leu Pro Gly Gly Arg Val Asn Gln Arg Asp Ala Asp Leu Val His
355 360 365
Phe Trp His Lys Tyr Arg Arg Ser Ala Glu Gly Ser Ala Lys Lys Gly
370 375 380
Glu Ala Arg Arg Arg Leu Val Glu Thr Met Ala Arg Arg Ser Arg Val
385 390 395 400
Asp Ser Ser Val Glu Leu Ile Gly Gly Leu Leu Phe Gly Ser Glu Gln
405 410 415
Gly Ala Lys Val Leu Gly Ala Val Arg Pro Ala Gly Gln Pro Val Val
420 425 430
Ala Asp Trp Asp Cys Leu Lys Ser Val Val Arg Arg Phe Gln Glu Arg
435 440 445
Cys Gly Pro Leu Thr Gln Tyr Gly Met Lys His Met Arg Ser Leu Ala
450 455 460
Asn Leu Cys Asn Ala Gly Val Arg Glu Glu Ala Met Asp Lys Ala Ala
465 470 475 480
Ala Gln Ala Cys Ala Ala Asn Pro Ser Ser Leu Phe
485 490
<210> 101
<211> 467
<212> PRT
<213> Brachypodium distachyon
<400> 101
Met Ala Arg Leu Ser Cys Phe Leu Leu Leu Leu Gln Ala Gln Leu Phe
1 5 10 15
Leu Leu Val Ala Gly Glu Phe Leu Arg Leu Pro Ser Glu Gln Asp Val
20 25 30
Ala Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Asn Asp Tyr Tyr
35 40 45
Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Ile Met Lys
50 55 60
Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp
65 70 75 80
Ile Ala Asn Asn Pro Asp Asn Pro Arg Pro Gly Val Ile Ile Asn His
85 90 95
Pro Thr Gly Gly Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly
100 105 110
Lys Asp Val Asn Ala Asn Asn Phe Leu Ala Ala Leu Leu Gly Asp Lys
115 120 125
Ser Lys Leu Thr Gly Ser Gly Ser Gly Lys Val Val Ser Ser Gly Pro
130 135 140
Asn Asp His Ile Phe Val Tyr Tyr Ala Asp His Gly Gly Pro Gly Val
145 150 155 160
Leu Gly Met Pro Glu Asp Glu Ser Tyr Leu Tyr Ala Asn Asp Leu Val
165 170 175
Arg Ala Leu Glu Lys Lys His Ala Gly Gly Ala Gly Tyr Lys Ser Leu
180 185 190
Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu
195 200 205
Leu Pro Gly Asn Ile Ser Val Tyr Ala Thr Thr Ala Ser Asn Ala Glu
210 215 220
Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Asp Val Asp Gly Ala Pro
225 230 235 240
Pro Ala Glu Phe Asp Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp
245 250 255
Met Glu Asp Ser Asp Ala His Asn Leu Lys Ala Glu Ser Leu Lys Gln
260 265 270
Gln Tyr Asp Arg Val Arg Asp Arg Thr Ser Ala His Glu Thr Tyr Asn
275 280 285
Leu Gly Ser His Val Met Gln Tyr Gly Asp Leu Gly Ile Asn Ala Gln
290 295 300
Ser Leu Asp Ile Phe Ile Gly Ser Asn Pro Ala Asn Asp Lys Ser Asn
305 310 315 320
Ser Ser Val Ser Ser Leu Leu Arg Asn Ala Arg Ala Gly Val Val His
325 330 335
Gln Arg Asp Ala Asp Leu Leu His Phe Trp His Lys Tyr Lys Arg Ser
340 345 350
Ala Glu Gly Ser Ala Arg Lys His Glu Ala Arg Arg Arg Leu Val Glu
355 360 365
Met Met Ala Arg Arg Ala Arg Val Asp Gly Ser Val Glu Leu Leu Gly
370 375 380
Gly Leu Leu Phe Gly Ser Glu Glu Gly Ala Lys Val Met Asn Ala Val
385 390 395 400
Arg Pro Ala Gly Gln Ala Leu Val Asp Asp Trp Asp Cys Leu Lys Asp
405 410 415
Val Val Arg Arg Phe Glu Ala Arg Cys Gly Pro Leu Thr Gln Tyr Gly
420 425 430
Met Lys His Met Arg Ala Leu Ala Asn Val Cys Asn Ala Gly Val Gly
435 440 445
Val Glu Ala Val Asp Arg Ala Ala Ser Gln Ala Cys Ala Val His Pro
450 455 460
Ser Val Phe
465
<210> 102
<211> 493
<212> PRT
<213> Sorghum bicolor
<400> 102
Met Ala Ala Ala Ala Trp Leu Cys Gly Leu Leu Trp Leu Leu Ala His
1 5 10 15
Ala Ala Ala Val Ala Ser Ala Ala Asp Gly Ala Asp Gly Gly Trp Glu
20 25 30
Pro Leu Ile Arg Met Pro Thr Gly Lys Gly Gly Asp Ala Ala Ala Arg
35 40 45
Ala Val Glu Glu Asp Asp Glu Val Gly Thr Arg Trp Ala Val Leu Val
50 55 60
Ala Gly Ser Ser Gly Tyr Gly Asn Tyr Arg His Gln Ala Asp Val Cys
65 70 75 80
His Ala Tyr Gln Ile Leu Arg Lys Gly Gly Val Lys Glu Glu Asn Ile
85 90 95
Val Val Phe Met Tyr Asp Asp Ile Ala His Asn Ile Leu Asn Pro Arg
100 105 110
Pro Gly Val Ile Ile Asn His Pro Lys Gly Glu Asn Val Tyr Asn Gly
115 120 125
Val Pro Lys Asp Tyr Thr Gly Asp Gln Val Thr Thr Glu Asn Phe Phe
130 135 140
Ala Val Leu Leu Gly Asn Lys Ser Ala Ile Thr Gly Gly Ser Lys Lys
145 150 155 160
Val Ile Asp Ser Lys Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp
165 170 175
His Gly Gly Pro Gly Val Leu Gly Met Pro Asn Leu Pro Tyr Leu Tyr
180 185 190
Ala Gly Asp Phe Ile Lys Val Leu Lys Lys Lys His Ala Cys Asn Ser
195 200 205
Tyr Ser Lys Met Val Ile Tyr Val Glu Ala Cys Glu Ser Gly Ser Ile
210 215 220
Phe Glu Gly Leu Met Pro Glu Asp Leu Asn Ile Tyr Val Thr Thr Ala
225 230 235 240
Ser Asn Pro Val Glu Asn Ser Trp Gly Thr Tyr Cys Pro Gly Met Glu
245 250 255
Pro Ser Pro Pro Pro Glu Tyr Ile Thr Cys Leu Gly Asp Leu Tyr Ser
260 265 270
Val Ser Trp Met Glu Asp Ser Gln Thr His Asn Leu Lys Lys Glu Thr
275 280 285
Ile Lys Asp Gln Tyr Glu Val Val Lys Thr Arg Thr Ser Asn Ser Asn
290 295 300
Lys Tyr Lys Glu Gly Ser His Val Met Glu Tyr Gly Asp Lys Thr Phe
305 310 315 320
Lys Asp Glu Lys Leu Phe Leu Tyr Gln Gly Phe Asp Pro Ala Asn Ala
325 330 335
Asn Ile Ala Asn Met Leu Leu Trp Pro Gly Pro Lys Gly Ala Val Asn
340 345 350
Gln Arg Asp Ala Asp Leu Leu Phe Met Trp Lys Arg Tyr Glu Gln Leu
355 360 365
Asn Gly Glu Ser Val Glu Lys Leu Arg Ala Leu Ile Glu Ile Lys Glu
370 375 380
Thr Val Gln His Arg Lys His Leu Asp Ser Ser Ile Asp Phe Ile Gly
385 390 395 400
Arg Leu Leu Phe Gly Phe Glu Lys Gly Pro Ser Met Leu Glu Ala Val
405 410 415
Arg Ala Ser Gly Leu Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Arg
420 425 430
Met Val Arg Ile Phe Glu Ser Gln Cys Gly Ser Leu Thr Gln Tyr Gly
435 440 445
Met Lys Tyr Met Arg Ala Phe Ala Asn Ile Cys Asn Ser Gly Ile Ser
450 455 460
Glu Met Lys Met Arg Glu Ser Ser Ile Ser Ala Cys Ser Ser Tyr Asn
465 470 475 480
Ser Ala Arg Trp Ser Pro Met Ala Gln Gly His Ser Ala
485 490
<210> 103
<211> 491
<212> PRT
<213> Triticum monococcum
<400> 103
Met Ala Pro Arg Trp Cys Phe Ala Leu Leu Leu Leu Leu Cys Ala Ala
1 5 10 15
Ala Arg Pro Gly Ala Asp Ala Ser Lys Gly Lys Trp Asp Pro Val Ile
20 25 30
Arg Met Pro Gly Glu Glu Glu Pro Ala Thr Gly Asp Glu Ser Ser Glu
35 40 45
Glu Gly Glu Asp Gly Val Gly Thr Arg Trp Ala Val Leu Val Ala Gly
50 55 60
Ser Ser Gly Tyr Gly Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala
65 70 75 80
Tyr Gln Ile Leu Arg Lys Gly Gly Val Lys Glu Glu Asn Ile Val Val
85 90 95
Phe Met Tyr Asp Asp Ile Ala Asn Asn Pro Leu Asn Pro Arg Pro Gly
100 105 110
Val Ile Ile Asn His Pro Glu Gly Glu Asp Val Tyr Ala Gly Val Pro
115 120 125
Lys Asp Tyr Thr Gly Glu Glu Val Thr Ala Lys Asn Phe Tyr Ala Val
130 135 140
Leu Leu Gly Asn Lys Thr Ala Val Thr Gly Gly Ser Lys Lys Val Ile
145 150 155 160
Asp Ser Lys Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His Gly
165 170 175
Gly Pro Gly Val Leu Gly Met Pro Asn Leu Pro Tyr Leu Tyr Ala Ala
180 185 190
Asp Phe Ile Lys Val Leu Gln Glu Lys His Ala Ser Asn Thr Tyr Ala
195 200 205
Lys Met Val Ile Tyr Val Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu
210 215 220
Gly Leu Met Pro Ala Asp Leu Asn Ile Tyr Val Thr Thr Ala Ser Asn
225 230 235 240
Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Met Glu Pro Ser
245 250 255
Pro Pro Ser Glu Tyr Ile Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ser
260 265 270
Trp Met Glu Asp Ser Glu Thr Asn Asn Leu Lys Glu Glu Thr Ile Lys
275 280 285
Lys Gln Tyr Glu Val Val Lys Lys Arg Thr Ser Asp Met Asn Ser Tyr
290 295 300
Ser Ala Gly Ser His Val Met Glu Tyr Gly Asp Lys Thr Phe Lys Asp
305 310 315 320
Glu Lys Leu Tyr Leu Tyr Gln Gly Phe Asn Pro Ala Asn Thr Asn Ile
325 330 335
Thr Asn Met Leu Leu Leu Gln Ala Pro Lys Ala Ala Ile Asn Gln Arg
340 345 350
Asp Ala Asp Leu Leu Phe Leu Trp Arg Arg Tyr Glu Leu Leu His Glu
355 360 365
Lys Ser Lys Glu Lys Gly Asn Val Leu Arg Glu Ile Ser Glu Thr Val
370 375 380
Thr His Arg Lys His Leu Asp Ser Ser Ile Asp Phe Ile Gly Lys Leu
385 390 395 400
Leu Phe Gly Phe Glu Asn Gly Pro Ser Val Leu Gln Ala Val Arg Pro
405 410 415
Ser Gly Lys Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Arg Met Val
420 425 430
Arg Ile Phe Glu Ser His Cys Gly Ser Leu Thr Gln Tyr Gly Met Lys
435 440 445
His Met Arg Ala Phe Ala Asn Ile Cys Asn Asn Gly Ile Ser Gly Thr
450 455 460
Thr Met Lys Glu Ala Ser Ile Gly Ala Cys Gly Val Gln Asn Ser Ala
465 470 475 480
Arg Trp Ser Ser Leu Ile Gln Gly Tyr Ser Ala
485 490
<210> 104
<211> 491
<212> PRT
<213> Aegilops speltoides
<400> 104
Met Ala Pro Arg Trp Cys Phe Ala Leu Leu Leu Leu Leu Cys Ala Ala
1 5 10 15
Ala Gly Ala Gly Ala Asp Ala Ser Lys Gly Lys Trp Asp Pro Val Ile
20 25 30
Arg Met Pro Gly Glu Glu Glu Pro Ala Thr Gly Asp Glu Ser Ser Glu
35 40 45
Glu Gly Glu Asp Gly Val Gly Thr Arg Trp Ala Val Leu Val Ala Gly
50 55 60
Ser Ser Gly Tyr Gly Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala
65 70 75 80
Tyr Gln Ile Leu Arg Lys Gly Gly Val Lys Glu Glu Asn Ile Val Val
85 90 95
Phe Met Tyr Asp Asp Ile Ala Asn Asn Pro Leu Asn Pro Arg Pro Gly
100 105 110
Val Ile Ile Asn His Pro Glu Gly Glu Asp Val Tyr Ala Gly Val Pro
115 120 125
Lys Asp Tyr Thr Gly Glu Ala Val Thr Ala Lys Asn Phe Tyr Ala Val
130 135 140
Leu Leu Gly Asn Asn Thr Ala Val Thr Gly Gly Ser Lys Lys Val Ile
145 150 155 160
Asp Ser Lys Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His Gly
165 170 175
Gly Pro Gly Val Leu Gly Met Pro Asn Leu Pro Tyr Leu Tyr Ala Ala
180 185 190
Asp Phe Ile Lys Val Leu Gln Glu Lys His Ala Ser Asn Thr Tyr Ala
195 200 205
Lys Met Val Ile Tyr Val Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu
210 215 220
Gly Leu Met Pro Ala Asp Leu Asn Ile Tyr Val Thr Thr Ala Ser Asn
225 230 235 240
Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Met Glu Pro Ser
245 250 255
Pro Pro Ser Glu Tyr Ile Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ser
260 265 270
Trp Met Glu Asp Ser Glu Thr Asn Asn Leu Lys Glu Glu Thr Ile Lys
275 280 285
Lys Gln Tyr Glu Val Val Lys Lys Arg Thr Ser Asp Met Asn Ser Tyr
290 295 300
Ser Ala Gly Ser His Val Met Glu Tyr Gly Asp Lys Thr Phe Lys Asp
305 310 315 320
Glu Lys Leu Tyr Leu Tyr Gln Gly Phe Asn Pro Ala Asn Thr Asn Ile
325 330 335
Thr Asn Lys Leu Phe Leu Gln Ala Pro Lys Ala Ala Ile Asn Gln Arg
340 345 350
Asp Ala Asp Leu Leu Phe Leu Trp Arg Arg Tyr Glu Leu Leu His Glu
355 360 365
Lys Ser Lys Glu Lys Ala Asn Val Leu Arg Glu Ile Ser Glu Thr Val
370 375 380
Ala His Arg Lys His Leu Asp Ser Ser Ile Asp Phe Ile Gly Lys Leu
385 390 395 400
Leu Phe Gly Phe Glu Asn Gly Pro Trp Glu Leu Gln Ala Val Arg Pro
405 410 415
Ser Gly Lys Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Arg Met Val
420 425 430
Arg Ile Phe Glu Ser His Cys Gly Ser Leu Thr Gln Tyr Gly Met Lys
435 440 445
His Met Arg Ala Phe Ala Asn Ile Cys Asn Asn Gly Val Ser Gly Thr
450 455 460
Thr Met Asn Glu Ala Ser Ile Gly Ala Cys Gly Val Gln Asn Ser Ala
465 470 475 480
Arg Trp Ser Thr Leu Ile Gln Gly Tyr Ser Ala
485 490
<210> 105
<211> 489
<212> PRT
<213> Aegilops tauschii
<400> 105
Met Ala Pro Arg Trp Cys Phe Ala Leu Leu Leu Leu Leu Cys Ala Ala
1 5 10 15
Ala Gly Ala Asp Ala Ser Lys Gly Lys Trp Asp Pro Val Ile Arg Met
20 25 30
Pro Gly Glu Glu Glu Pro Ala Thr Gly Asp Asp Ser Ser Glu Glu Gly
35 40 45
Glu Asp Gly Val Gly Thr Arg Trp Ala Val Leu Val Ala Gly Ser Ser
50 55 60
Gly Tyr Gly Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln
65 70 75 80
Ile Leu Arg Lys Gly Gly Val Lys Glu Glu Asn Ile Val Val Phe Met
85 90 95
Tyr Asp Asp Ile Ala Asn Asn Pro Leu Asn Pro Arg Pro Gly Val Ile
100 105 110
Ile Asn His Pro Glu Gly Glu Asp Val Tyr Ala Gly Val Pro Lys Asp
115 120 125
Tyr Thr Gly Glu Ala Val Thr Ala Lys Asn Phe Tyr Ala Val Leu Leu
130 135 140
Gly Asn Lys Thr Ala Val Thr Gly Gly Ser Lys Lys Val Ile Asp Ser
145 150 155 160
Lys Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His Gly Gly Pro
165 170 175
Gly Val Leu Gly Met Pro Asn Leu Pro Tyr Leu Tyr Ala Ala Asp Phe
180 185 190
Ile Lys Val Leu Gln Glu Lys His Ala Ser Asn Thr Tyr Ala Lys Met
195 200 205
Val Ile Tyr Val Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu
210 215 220
Met Pro Ala Asp Leu Asn Ile Tyr Val Thr Thr Ala Ser Asn Ala Glu
225 230 235 240
Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Met Glu Pro Ser Pro Pro
245 250 255
Ser Glu Tyr Ile Thr Cys Leu Gly Asp Leu Tyr Ser Ile Ser Trp Met
260 265 270
Glu Asp Ser Glu Thr Asn Asn Leu Lys Glu Glu Thr Ile Lys Lys Gln
275 280 285
Tyr Glu Val Val Lys Lys Arg Thr Ser Asp Met Asn Ser Tyr Ser Ala
290 295 300
Gly Ser His Val Met Glu Tyr Gly Asp Met Thr Phe Lys Asp Glu Lys
305 310 315 320
Leu Tyr Leu Tyr Gln Gly Phe Asn Pro Ala Asn Thr Asn Ile Thr Asn
325 330 335
Lys Leu Phe Leu Gln Ala Pro Lys Ala Ala Ile Asn Gln Arg Asp Ala
340 345 350
Asp Leu Leu Phe Leu Trp Arg Arg Tyr Glu Leu Leu His Gly Lys Ser
355 360 365
Lys Glu Lys Ala Asn Val Leu Thr Glu Ile Gly Glu Thr Val Ala His
370 375 380
Arg Lys His Leu Asp Asn Ser Ile Asp Phe Ile Gly Lys Leu Leu Phe
385 390 395 400
Gly Phe Glu Asn Gly Pro Ser Glu Leu Gln Ala Val Arg Pro Ser Gly
405 410 415
Lys Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Arg Met Val Arg Ile
420 425 430
Phe Glu Ser His Cys Gly Ser Leu Thr Gln Tyr Gly Met Lys His Met
435 440 445
Arg Ala Phe Ala Asn Ile Cys Asn Asn Gly Val Ser Gly Thr Thr Met
450 455 460
Lys Glu Ala Ser Ile Asn Thr Cys Gly Gly His Asn Ser Ala Arg Leu
465 470 475 480
Ser Thr Leu Ile Gln Gly Tyr Ser Ala
485
<210> 106
<211> 460
<212> PRT
<213> Solanum tuberosum
<400> 106
Met Ile Phe Lys Tyr Asn Val Phe Val Ala Leu Val Val Val Leu Ser
1 5 10 15
Ile Trp Asp Asn Ile Glu Gly Arg Ser Val Ser Lys Phe Leu Thr Glu
20 25 30
Glu Thr Val Gly Thr Lys Trp Ala Val Leu Val Ala Gly Ser Asn Gly
35 40 45
Trp Phe Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Ile
50 55 60
Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr
65 70 75 80
Asp Asp Ile Ala Asn Asn Thr Met Asn Pro Arg Pro Gly Val Ile Ile
85 90 95
Asn Asn Pro His Gly Gln Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr
100 105 110
Val Gly Glu Asp Val Asn Ala Glu Asn Phe Phe Asn Val Ile Leu Ala
115 120 125
Asn Lys Ser Gly Ile Thr Gly Gly Ser Gly Lys Val Leu Asn Ser Gly
130 135 140
Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His Gly Gly Pro Gly
145 150 155 160
Ile Ile Ser Met Pro Thr Gly Leu Val Tyr Ala Asn Asp Leu Ile Asn
165 170 175
Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Ser Lys Leu Val Phe
180 185 190
Tyr Leu Glu Ala Cys Glu Ser Gly Ser Met Phe Asp Gly Leu Leu Pro
195 200 205
Glu Gly Leu Asn Ile Tyr Val Thr Thr Ala Ser Asn Pro Asn Glu Ser
210 215 220
Ser Trp Gly Thr Tyr Cys Gln Met Gly Ala Gly Gly Pro Cys Leu Val
225 230 235 240
Glu Cys Pro Pro Pro Glu Phe Gln Gly Val Cys Leu Gly Asp Leu Tyr
245 250 255
Ser Val Ala Trp Met Glu Asp Ser Glu Ala Glu Asp Arg Gln Thr Glu
260 265 270
Thr Leu Asn Asp Gln Tyr Asn Thr Val Ala Asn Arg Thr Ala Ala Asn
275 280 285
Leu Thr Tyr Gly Ser His Val Met Gln Tyr Gly Asp Thr Val Leu Ser
290 295 300
Val Asp Val Leu Phe Gln Tyr Met Gly Ala Ala Ser Val Asn His Ser
305 310 315 320
His Val Ser Met Asn Ser Glu Ser Ser Ser Gln Asn Val Asp Gln Arg
325 330 335
Asp Val Glu Leu Phe Tyr Leu Thr Ser Lys Tyr Gln Asp Ala Pro Glu
340 345 350
Gly Ser Asp Glu His Phe Glu Thr Arg Val Lys Leu Ile Lys Thr Ile
355 360 365
Ala Glu Arg Ser Gln Val Asp Asn Ser Val Lys His Ile Gly Asp Leu
370 375 380
Leu Phe Gly Val Glu Lys Gly Ser Glu Val Leu Gln His Val Arg Pro
385 390 395 400
Ala Gly Gln Pro Leu Val Asp Asn Trp Asp Cys Leu Lys Ser Tyr Ile
405 410 415
Glu Thr Phe Glu Val His Cys Gly Lys Leu Ser Ser Tyr Gly Lys Lys
420 425 430
His Ile Arg Gly Ile Ala Asn Ile Cys Asn Ala Gly Ile Lys Ser Glu
435 440 445
Gln Met Ala Ser Ala Thr Ala Gln Ala Cys Ser Ser
450 455 460
<210> 107
<211> 490
<212> PRT
<213> Brachypodium distachyon
<400> 107
Met Ala Ala Trp Trp Cys Phe Ala Leu Leu Leu Val Leu Cys Ala Pro
1 5 10 15
Ala Gly Ala Asp Val Ser Lys Gly Lys Trp Glu Pro Leu Ile Arg Met
20 25 30
Pro Gly Glu Lys Glu Pro Ala Thr Ala Arg Gly Phe Glu Gly Pro Glu
35 40 45
Glu Glu Asp Gly Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser
50 55 60
Ser Gly Tyr Gly Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr
65 70 75 80
Gln Val Leu Arg Lys Gly Gly Leu Lys Glu Glu Asn Ile Val Val Phe
85 90 95
Met Tyr Asp Asp Ile Ala Asn Ser Ala Leu Asn Pro Arg Pro Gly Val
100 105 110
Ile Ile Asn His Pro Gln Gly Glu Asp Val Tyr Ala Gly Val Pro Lys
115 120 125
Asp Tyr Thr Gly Glu Gln Val Thr Ala Lys Asn Leu Tyr Ala Val Leu
130 135 140
Leu Gly Asn Lys Thr Ala Val Thr Gly Gly Ser Lys Lys Val Ile Asp
145 150 155 160
Ser Gln Pro Lys Asp His Ile Phe Ile Tyr Tyr Ser Asp His Gly Gly
165 170 175
Pro Gly Val Leu Gly Met Pro Asn Leu Pro Tyr Leu Tyr Ala Gly Asp
180 185 190
Phe Ile Lys Ile Leu Gln Gln Lys His Ala Ser Asn Thr Tyr Ala Lys
195 200 205
Met Val Ile Tyr Val Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly
210 215 220
Leu Met Pro Ala Asp Leu Asn Ile Tyr Val Thr Thr Ala Ser Asn Ala
225 230 235 240
Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Met Glu Pro Ser Pro
245 250 255
Pro Ser Glu Tyr Ile Thr Cys Leu Gly Asp Leu Tyr Ser Val Ser Trp
260 265 270
Met Glu Asp Ser Glu Asn His Asn Leu Lys Glu Glu Thr Ile Lys Lys
275 280 285
Gln Tyr Glu Val Val Lys Arg Arg Thr Ser Asp Leu Asn Ser Tyr Ser
290 295 300
Ala Gly Ser His Val Met Glu Tyr Gly Asp Lys Thr Phe Lys Asp Glu
305 310 315 320
Lys Leu Tyr Leu Tyr Gln Gly Phe Asn Pro Ala Asn Ala Asn Ile Thr
325 330 335
Asn Lys Leu Phe Trp Gln Ala Pro Arg Ala Ala Ile Asn Gln Arg Asp
340 345 350
Ala Asp Leu Leu Phe Leu Trp Arg Arg Tyr Glu Met Leu His Glu Lys
355 360 365
Ser Lys Glu Lys Val Lys Val Leu Arg Glu Ile Ser Glu Thr Val Met
370 375 380
His Arg Lys His Leu Asp Asn Ser Val Asp Leu Ile Gly Gln Leu Leu
385 390 395 400
Phe Gly Phe Glu Asn Gly Pro Ser Val Leu Gln Ala Val Arg Pro Ser
405 410 415
Gly Lys Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Arg Met Val Arg
420 425 430
Ile Phe Glu Ser His Cys Gly Pro Leu Thr Gln Tyr Gly Met Lys His
435 440 445
Met Arg Ala Phe Ala Asn Ile Cys Asn Asn Gly Ile Pro Gly Ser Thr
450 455 460
Met Lys Glu Gly Ser Ile Ser Ala Cys Gly Ser Arg Asn Ile Ala Arg
465 470 475 480
Trp Ser Pro Leu Ile Gln Gly Tyr Ser Ala
485 490
<210> 108
<211> 493
<212> PRT
<213> Zea mays
<400> 108
Met Met Ala Ala Ala Ala Trp Leu Cys Gly Leu Leu Ser Leu Leu Ala
1 5 10 15
Leu Ala Gly Ala Ala Ser Ala Ala Asp Gly Ala Glu Gly Glu Trp Glu
20 25 30
Pro Leu Ile Arg Met Pro Thr Ala Lys Gly Ser Asp Ala Ala Ser Ala
35 40 45
Pro Ala Ala Glu Asp Asp Glu Val Gly Thr Arg Trp Ala Val Leu Val
50 55 60
Ala Gly Ser Phe Gly Tyr Gly Asn Tyr Arg His Gln Ala Asp Val Cys
65 70 75 80
His Ala Tyr Gln Ile Leu Gln Lys Gly Gly Val Lys Lys Glu Asn Ile
85 90 95
Val Val Phe Met Tyr Asp Asp Ile Ala His Asn Ile Leu Asn Pro Arg
100 105 110
Pro Gly Val Ile Ile Asn His Pro Lys Gly Ala Asn Val Tyr Asp Gly
115 120 125
Val Pro Lys Asp Tyr Thr Gly Asp Gln Val Thr Thr Glu Asn Phe Phe
130 135 140
Ala Val Leu Leu Gly Asn Arg Ser Ala Thr Thr Gly Gly Ser Lys Lys
145 150 155 160
Val Ile Asp Ser Lys Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp
165 170 175
His Gly Gly Pro Gly Val Leu Gly Met Pro Asn Leu Pro Tyr Leu Tyr
180 185 190
Ala Gly Asp Phe Ile Lys Val Leu Lys Lys Lys His Ala Ser Asn Ser
195 200 205
Tyr Ser Lys Met Val Ile Tyr Val Glu Ala Cys Glu Ser Gly Ser Ile
210 215 220
Phe Glu Gly Leu Met Pro Glu Asp Leu Asn Ile Tyr Val Thr Thr Ala
225 230 235 240
Ser Asn Pro Val Glu Asn Ser Trp Gly Thr Tyr Cys Pro Gly Met Glu
245 250 255
Pro Ser Pro Pro Pro Glu Tyr Ile Thr Cys Leu Gly Asp Leu Tyr Ser
260 265 270
Val Ser Trp Met Glu Asp Ser Glu Thr His Asn Leu Lys Lys Glu Thr
275 280 285
Ile Lys Asp Gln Tyr Glu Val Val Lys Thr Arg Thr Ser Asn Ser Asn
290 295 300
Lys Tyr Lys Glu Gly Ser His Val Met Glu Tyr Gly Asp Lys Thr Phe
305 310 315 320
Lys Asp Glu Lys Leu Ser Phe Tyr Gln Gly Phe Asp Pro Ala Asn Ala
325 330 335
Asn Ile Ala Asn Met Leu Leu Trp Pro Gly Pro Lys Gly Ala Val Asn
340 345 350
Gln Arg Asp Ala Asp Leu Leu Phe Met Trp Lys Arg Tyr Glu Gln Leu
355 360 365
Asn Gly Gly Thr Glu Glu Lys Leu Arg Ala Leu Ile Glu Ile Lys Glu
370 375 380
Thr Val Gln His Arg Lys His Leu Asp Ser Ser Ile Asp Phe Val Gly
385 390 395 400
Arg Leu Val Phe Gly Phe Glu Lys Gly Pro Ser Met Leu Glu Ala Val
405 410 415
Arg Thr Ser Gly Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Arg
420 425 430
Met Val Arg Ile Phe Glu Ser Gln Cys Gly Ser Leu Thr Gln Tyr Gly
435 440 445
Met Lys Tyr Met Arg Ala Phe Ala Asn Ile Cys Asn Ser Gly Ile Ser
450 455 460
Glu Met Lys Met Arg Glu Ser Ser Ile Ser Ala Cys Ser Ser Tyr Asn
465 470 475 480
Ser Ala Arg Trp Ser Pro Met Ala Arg Gly His Ser Ala
485 490
<210> 109
<211> 492
<212> PRT
<213> Setaria italica
<400> 109
Met Ala Ala Ala Ala Trp Leu Cys Gly Leu Leu Ser Leu Leu Ala Val
1 5 10 15
Ala Ala Ala Ala Ser Val Asp Gly Ala Glu Glu Glu Trp Glu Pro Leu
20 25 30
Ile Arg Met Pro Thr Glu Lys Gly Gly Asn Ala Ala Ala Ala Ala Pro
35 40 45
Ala Ala Glu Glu Asp Glu Val Gly Thr Arg Trp Ala Val Leu Val Ala
50 55 60
Gly Ser Ser Gly Tyr Gly Asn Tyr Arg His Gln Ala Asp Val Cys His
65 70 75 80
Ala Tyr Gln Ile Leu Leu Lys Gly Gly Val Lys Glu Glu Asn Ile Val
85 90 95
Val Phe Met Tyr Asp Asp Ile Ala His Asn Ile Leu Asn Pro Arg Pro
100 105 110
Gly Val Ile Ile Asn His Pro Lys Gly Glu Asn Val Tyr Pro Gly Val
115 120 125
Pro Lys Asp Tyr Thr Gly Asp Gln Val Thr Thr Glu Asn Phe Phe Ala
130 135 140
Val Leu Leu Gly Asn Arg Ser Ala Ile Thr Gly Gly Ser Lys Lys Val
145 150 155 160
Ile Asp Ser Lys Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His
165 170 175
Gly Gly Pro Gly Val Leu Gly Met Pro Asn Leu Pro Tyr Leu Tyr Ala
180 185 190
Gly Asp Phe Ile Lys Val Leu Lys Lys Lys His Ala Ser Asn Ser Tyr
195 200 205
Ser Lys Met Val Ile Tyr Val Glu Ala Cys Glu Ser Gly Ser Ile Phe
210 215 220
Glu Gly Leu Met Pro Gln Asp Leu Asn Ile Tyr Val Thr Thr Ala Ser
225 230 235 240
Asn Pro Val Glu Asn Ser Trp Gly Thr Tyr Cys Pro Gly Met Asp Pro
245 250 255
Ser Pro Pro Pro Glu Tyr Ile Thr Cys Leu Gly Asp Leu Tyr Ser Val
260 265 270
Ser Trp Met Glu Asp Ser Gln Thr His Asn Leu Met Lys Glu Thr Ile
275 280 285
Lys Asp Gln Tyr Glu Val Val Lys Thr Arg Thr Ser Asn Leu Lys Lys
290 295 300
Tyr Lys Glu Gly Ser His Val Met Glu Tyr Gly Asp Lys Thr Phe Thr
305 310 315 320
Asn Glu Lys Leu Phe Leu Tyr Gln Gly Phe Asp Pro Ala Asn Ala Asn
325 330 335
Ala Ala Asn Thr Leu Leu Trp Pro Gly Pro Lys Gly Ala Val Asn Gln
340 345 350
Arg Asp Ala Asp Leu Leu Phe Met Trp Lys Arg Tyr Glu Gln Leu Asp
355 360 365
Gly Gly Ser Glu Glu Lys Leu Arg Ala Leu Arg Glu Ile Lys Glu Thr
370 375 380
Val Gln His Arg Lys His Leu Asp Ser Ser Ile Asp Phe Ile Gly Arg
385 390 395 400
Leu Val Phe Gly Phe Glu Asn Gly Pro Lys Met Leu Glu Ala Val Arg
405 410 415
Ala Ser Gly Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys Arg Met
420 425 430
Val Arg Ile Phe Glu Ala Gln Cys Gly Ser Leu Thr Gln Tyr Gly Met
435 440 445
Lys Tyr Met Arg Ala Phe Ala Asn Ile Cys Asn Ser Gly Ile Ser Glu
450 455 460
Ala Lys Met Arg Glu Ser Ser Ile Ser Ala Cys Gly Gly Tyr Asn Ser
465 470 475 480
Ala Arg Trp Ser Pro Met Ala Gln Gly His Ser Ala
485 490
<210> 110
<211> 31
<212> PRT
<213> Oldenlandia affinis
<400> 110
Gly Leu Pro Val Cys Gly Glu Thr Cys Val Gly Gly Thr Cys Asn Thr
1 5 10 15
Pro Gly Cys Thr Cys Ser Trp Pro Val Cys Thr Arg Asn His Val
20 25 30
<210> 111
<211> 17
<212> PRT
<213> Conus sp.
<400> 111
Gly Val Cys Cys Gly Tyr Lys Leu Cys His Pro Cys Ala Gly Asn His
1 5 10 15
Val
<210> 112
<211> 25
<212> PRT
<213> 人工的
<220>
<223> Thanatin analog
<400> 112
Gly Ile Ser Lys Lys Pro Val Pro Ile Ile Tyr Cys Asn Arg Arg Thr
1 5 10 15
Gly Lys Cys Gln Arg Met Asn His Val
20 25
<210> 113
<211> 42
<212> PRT
<213> 人
<400> 113
Ser Ala Asp Ser His Glu Lys Arg His His Gly Tyr Arg Arg Lys Phe
1 5 10 15
His Glu Lys His His Ser His Arg Glu Phe Pro Phe Tyr Gly Asp Tyr
20 25 30
Gly Ser Asn Tyr Leu Tyr Asp Asn His Val
35 40
<210> 114
<211> 43
<212> PRT
<213> 人
<400> 114
Gly Leu Pro Asp Ser His Glu Lys Arg His His Gly Tyr Arg Arg Lys
1 5 10 15
Phe His Glu Lys His His Ser His Arg Glu Phe Pro Phe Tyr Gly Asp
20 25 30
Tyr Gly Ser Asn Tyr Leu Tyr Asp Asn His Val
35 40
<210> 115
<211> 36
<212> PRT
<213> 人
<400> 115
Gly Ala Asp Ser His Ala Lys Arg His His Gly Tyr Lys Arg Lys Phe
1 5 10 15
His Glu Lys His His Ser His Arg Gly Tyr Arg Ser Asn Tyr Leu Tyr
20 25 30
Asp Asn His Val
35
<210> 116
<211> 36
<212> PRT
<213> 人
<400> 116
Gly Leu Asp Ser His Ala Lys Arg His His Gly Tyr Lys Arg Lys Phe
1 5 10 15
His Glu Lys His His Ser His Arg Gly Tyr Arg Ser Asn Tyr Leu Tyr
20 25 30
Asp Asn His Val
35
<210> 117
<211> 10
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 117
Gly Leu Pro Pro Pro Ile Phe Asn His Val
1 5 10
<210> 118
<211> 10
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 118
Ser Leu Pro Pro Pro Ile Phe Asn His Val
1 5 10
<210> 119
<211> 10
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 119
His Leu Pro Pro Pro Ile Phe Asn His Val
1 5 10
<210> 120
<211> 10
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 120
Glu Ile Asn Ser Thr Glu Ile Asn His Val
1 5 10
<210> 121
<211> 9
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 121
Arg Val Thr Arg Pro Val Asn His Val
1 5
<210> 122
<211> 8
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 122
Lys Ala Leu Val Ile Asn His Val
1 5
<210> 123
<211> 6
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<220>
<221> misc_feature
<222> (1)..(1)
<223> Xaa可以是任何天然存在的氨基酸
<400> 123
Xaa Ile Gly Gly Ile Arg
1 5
<210> 124
<211> 6
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<220>
<221> misc_feature
<222> (2)..(2)
<223> Xaa可以是任何天然存在的氨基酸
<400> 124
Leu Xaa Gly Gly Ile Arg
1 5
<210> 125
<211> 5
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 125
Tyr Arg Asn His Val
1 5
<210> 126
<211> 5
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 126
Gly Leu Pro Val Arg
1 5
<210> 127
<211> 5
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 127
Thr Arg Asn His Val
1 5
<210> 128
<211> 26
<212> PRT
<213> 人工的
<220>
<223> 经修饰的人类神经介肽U
<400> 128
Arg Val Asp Glu Glu Phe Gln Ser Pro Phe Ala Ser Gln Ser Arg Gly
1 5 10 15
Tyr Phe Leu Phe Arg Pro Arg Asn His Val
20 25
<210> 129
<211> 33
<212> PRT
<213> 人工的
<220>
<223> 经修饰的人类心血管调节肽alpha
<400> 129
Gly Ile Ser Gly Ala Leu Pro Pro Ala Pro Ala Ala Pro Arg Pro Ala
1 5 10 15
Leu Arg Ala Gln Arg Ala Gly Pro Ala Gly Pro Gly Ala Lys Asn His
20 25 30
Val
<210> 130
<211> 28
<212> PRT
<213> 人工的
<220>
<223> 经修饰的大鼠神经介肽U
<400> 130
Gly Ile Lys Tyr Lys Val Asn Glu Tyr Gln Gly Pro Val Ala Pro Ser
1 5 10 15
Gly Gly Phe Phe Leu Phe Arg Pro Arg Asn His Val
20 25
<210> 131
<211> 40
<212> PRT
<213> 人工的
<220>
<223> 经修饰的人apelin
<400> 131
Gly Leu Val Gln Pro Arg Gly Ser Arg Asn Gly Pro Gly Pro Trp Gln
1 5 10 15
Gly Gly Arg Arg Lys Phe Arg Arg Gln Arg Pro Arg Leu Ser His Lys
20 25 30
Gly Pro Met Pro Phe Asn His Val
35 40
<210> 132
<211> 32
<212> PRT
<213> 人工的
<220>
<223> 经修饰的人甘丙肽
<400> 132
Gly Leu Thr Ser Gly Trp Thr Leu Asn Ser Ala Gly Tyr Leu Leu Gly
1 5 10 15
Pro His Ala Val Gly Asn His Arg Ser Phe Ser Asp Lys Asn His Val
20 25 30
<210> 133
<211> 263
<212> PRT
<213> 人工的
<220>
<223> GFP + NHV tag
<400> 133
Met His His His His His His Ser Ser Gly Val Asp Leu Gly Thr Glu
1 5 10 15
Asn Leu Tyr Phe Gln Ser Met Ser Lys Gly Glu Glu Leu Phe Thr Gly
20 25 30
Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys
35 40 45
Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu
50 55 60
Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro
65 70 75 80
Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr
85 90 95
Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu
100 105 110
Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr
115 120 125
Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg
130 135 140
Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly
145 150 155 160
His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala
165 170 175
Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn
180 185 190
Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr
195 200 205
Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser
210 215 220
Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met
225 230 235 240
Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp
245 250 255
Glu Leu Tyr Lys Asn His Val
260
<210> 134
<211> 5
<212> PRT
<213> 人工的
<220>
<223> 4位的K被生物素化
<400> 134
Gly Ile Gly Lys Arg
1 5
<210> 135
<211> 16
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 135
Gly Arg Cys Thr Lys Ser Ile Pro Pro Ile Cys Phe Pro Asn His Val
1 5 10 15
<210> 136
<211> 32
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 136
Gly Leu Pro Val Cys Gly Glu Thr Cys Val Gly Gly Thr Cys Asn Thr
1 5 10 15
Pro Gly Cys Thr Cys Ser Trp Pro Val Cys Thr Arg Asn His Val Ile
20 25 30
<210> 137
<211> 33
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 137
Gly Leu Pro Val Cys Gly Glu Thr Cys Val Gly Gly Thr Cys Asn Thr
1 5 10 15
Pro Gly Cys Thr Cys Ser Trp Pro Val Cys Thr Arg Asn His Val Ile
20 25 30
Ala
<210> 138
<211> 30
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 138
Gly Leu Pro Val Cys Gly Glu Thr Cys Val Gly Gly Thr Cys Asn Thr
1 5 10 15
Pro Gly Cys Thr Cys Ser Trp Pro Val Cys Thr Arg Asn His
20 25 30
<210> 139
<211> 29
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 139
Gly Leu Pro Val Cys Gly Glu Thr Cys Val Gly Gly Thr Cys Asn Thr
1 5 10 15
Pro Gly Cys Thr Cys Ser Trp Pro Val Cys Thr Arg Asn
20 25
<210> 140
<211> 32
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 140
Gly Gly Leu Pro Val Cys Gly Glu Thr Cys Val Gly Gly Thr Cys Asn
1 5 10 15
Thr Pro Gly Cys Thr Cys Ser Trp Pro Val Cys Thr Arg Asp His Val
20 25 30
<210> 141
<211> 32
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 141
Gly Gly Leu Pro Val Cys Gly Glu Thr Cys Val Gly Gly Thr Cys Asn
1 5 10 15
Thr Pro Gly Cys Thr Cys Ser Trp Pro Val Cys Thr Arg Ala His Val
20 25 30
<210> 142
<211> 32
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 142
Gly Gly Leu Pro Val Cys Gly Glu Thr Cys Val Gly Gly Thr Cys Asn
1 5 10 15
Thr Pro Gly Cys Thr Cys Ser Trp Pro Val Cys Thr Arg Gln His Val
20 25 30
<210> 143
<211> 32
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 143
Gly Gly Leu Pro Val Cys Gly Glu Thr Cys Val Gly Gly Thr Cys Asn
1 5 10 15
Thr Pro Gly Cys Thr Cys Ser Trp Pro Val Cys Thr Arg Glu His Val
20 25 30
<210> 144
<211> 16
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 144
Gly Arg Cys Thr Lys Ser Ile Pro Pro Ile Cys Phe Pro Asp His Val
1 5 10 15
<210> 145
<211> 244
<212> PRT
<213> 人工的
<220>
<223> 经修饰的GFP
<400> 145
Gly Ile Ser Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro
1 5 10 15
Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val
20 25 30
Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys
35 40 45
Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val
50 55 60
Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His
65 70 75 80
Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val
85 90 95
Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg
100 105 110
Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu
115 120 125
Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu
130 135 140
Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln
145 150 155 160
Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp
165 170 175
Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly
180 185 190
Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser
195 200 205
Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu
210 215 220
Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr
225 230 235 240
Lys Asn His Val
<210> 146
<211> 129
<212> PRT
<213> 人工的
<220>
<223> 人抗-ABL scFv片段
<400> 146
Met His His His His His His Ser Ser Gly Val Asp Leu Gly Thr Glu
1 5 10 15
Asn Leu Tyr Phe Gln Ser Met Gly Gly Ser Gly Ser Ser Val Ser Ser
20 25 30
Val Pro Thr Lys Leu Glu Val Val Asp Ala Thr Pro Thr Ser Leu Lys
35 40 45
Ile Ser Trp Asp Ala Tyr Tyr Ser Ser Trp Gln Asn Val Lys Tyr Tyr
50 55 60
Arg Ile Thr Tyr Gly Glu Thr Gly Gly Asp Ser Pro Val Gln Glu Phe
65 70 75 80
Thr Val Pro Gly Tyr Tyr Ser Thr Ala Thr Ile Ser Gly Leu Lys Pro
85 90 95
Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Tyr Asp Thr Phe Phe Pro
100 105 110
Gly Tyr Glu Pro Asn Ser Pro Ile Ser Ile Asn Tyr Arg Thr Asn His
115 120 125
Val
<210> 147
<211> 184
<212> PRT
<213> 人工的
<220>
<223> 对ERK特异性的darpin
<400> 147
Met His His His His His His Ser Ser Gly Val Asp Leu Gly Thr Glu
1 5 10 15
Asn Leu Tyr Phe Gln Ser Met Gly Ser Asp Leu Gly Lys Lys Leu Leu
20 25 30
Glu Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg Ile Leu Met Ala
35 40 45
Asn Gly Ala Asp Val Asn Ala His Asp Asp Gln Gly Ser Thr Pro Leu
50 55 60
His Leu Ala Ala Trp Ile Gly His Pro Glu Ile Val Glu Val Leu Leu
65 70 75 80
Lys His Gly Ala Asp Val Asn Ala Arg Asp Thr Asp Gly Trp Thr Pro
85 90 95
Leu His Leu Ala Ala Asp Asn Gly His Leu Glu Ile Val Glu Val Leu
100 105 110
Leu Lys Tyr Gly Ala Asp Val Asn Ala Gln Asp Ala Tyr Gly Leu Thr
115 120 125
Pro Leu His Leu Ala Ala Asp Arg Gly His Leu Glu Ile Val Glu Val
130 135 140
Leu Leu Lys His Gly Ala Asp Val Asn Ala Gln Asp Lys Phe Gly Lys
145 150 155 160
Thr Ala Phe Asp Ile Ser Ile Asp Asn Gly Asn Glu Asp Leu Ala Glu
165 170 175
Ile Leu Gln Lys Leu Asn His Val
180
<210> 148
<211> 84
<212> PRT
<213> 人工的
<220>
<223> 泛素
<400> 148
Gly Ile Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr
1 5 10 15
Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile
20 25 30
Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala
35 40 45
Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln
50 55 60
Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly His His
65 70 75 80
His His His His
<210> 149
<211> 5
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 149
Tyr Lys Asn His Val
1 5
<210> 150
<211> 5
<212> PRT
<213> 人工的
<220>
<223> 人工肽
<400> 150
Gly Lys Asn His Val
1 5
<210> 151
<211> 29
<212> PRT
<213> 人工的
<220>
<223> 环化的kB1片段
<400> 151
Asn Gly Leu Pro Val Cys Gly Glu Thr Cys Val Gly Gly Thr Cys Asn
1 5 10 15
Thr Pro Gly Cys Thr Cys Ser Trp Pro Val Cys Thr Arg
20 25
<210> 152
<211> 22
<212> PRT
<213> 人工的
<220>
<223> 片段肽
<400> 152
Thr Ile Val Ala Leu Ile Glu Asp Gly Thr His Val Val Gln Tyr Gly
1 5 10 15
Asp Val Gly Leu Ser Lys
20
<210> 153
<211> 13
<212> PRT
<213> 人工的
<220>
<223> 肽片段
<400> 153
His Gln Ala Asp Val Cys His Ala Tyr Gln Leu Ile Lys
1 5 10
<210> 154
<211> 9
<212> PRT
<213> 人工的
<220>
<223> 肽片段
<400> 154
Trp Ala Val Leu Val Ala Gly Ser Lys
1 5
<210> 155
<211> 6
<212> PRT
<213> 人工的
<220>
<223> 肽片段
<400> 155
Gly Tyr Val Asn Tyr Arg
1 5
<210> 156
<211> 7
<212> PRT
<213> 人工的
<220>
<223> 肽片段
<400> 156
His Ala Ser Gly Thr Tyr Lys
1 5
<210> 157
<211> 14
<212> PRT
<213> 人工的
<220>
<223> 肽文库1
<220>
<221> misc_feature
<222> (1)..(1)
<223> Xaa可以是任何天然存在的氨基酸
<400> 157
Xaa Leu Tyr Arg Arg Gly Arg Tyr Leu Arg Arg Asn His Val
1 5 10
<210> 158
<211> 14
<212> PRT
<213> 人工的
<220>
<223> 肽文库2
<220>
<221> misc_feature
<222> (1)..(1)
<223> Xaa可以是任何天然存在的氨基酸
<400> 158
Xaa Arg Leu Tyr Arg Gly Arg Tyr Leu Arg Arg Asn His Val
1 5 10
<210> 159
<211> 14
<212> PRT
<213> 人工的
<220>
<223> 肽文库3
<220>
<221> misc_feature
<222> (2)..(2)
<223> Xaa可以是任何天然存在的氨基酸
<400> 159
Gly Xaa Leu Tyr Arg Gly Arg Tyr Leu Arg Arg Asn His Val
1 5 10

Claims (36)

1.具有蛋白质连接酶、优选环化酶活性的分离的多肽，包含以下项或由其组成
(i)如在SEQ ID NO:1中列出的氨基酸序列；
(ii)在其整个长度上与在SEQ ID NO:1中列出的氨基酸序列具有至少60％、优选至少70％、更优选至少80％、最优选至少90％序列同一性的氨基酸序列；
(iii)在其整个长度上与在SEQ ID No:1中列出的氨基酸序列具有至少80％、优选至少90％、更优选至少95％序列同源性的氨基酸序列；或
(iv)(i)-(iii)中任一项的片段。
2.如权利要求1所述的分离的多肽，其中所述分离的多肽包含SEQ ID NO:2中列出的氨基酸序列或由其组成。
3.如权利要求1或2所述的分离的多肽，其中所述多肽包含
(i)对应于SEQ ID NO:1的位置19的位置处的氨基酸残基N；和/或
(ii)对应于SEQ ID NO:1的位置124的位置处的氨基酸残基H；和/或
(iii)对应于SEQ ID NO:1的位置166的位置处的氨基酸残基Cat。
4.如权利要求1-3中任一项所述的分离的多肽，其中所述多肽能以80％或更高、优选90％或更高的效率环化给定肽。
5.如权利要求1-4中任一项所述的分离的多肽，其中所述多肽能以
(i)500μM或更小、优选250μM或更小的Km；和/或
(ii)至少0.05s-1、优选至少0.5s-1、更优选至少1.0s-1、最优选至少1.5s-1的kcat
环化给定肽。
6.如权利要求1至5中任一项所述的分离的多肽，其中所述多肽被糖基化。
7.编码根据权利要求1至6中任一项所述的多肽的核酸分子。
8.如权利要求7所述的核酸分子，其中所述核酸分子被包含在载体中。
9.如权利要求8所述的核酸分子，其中所述载体进一步包含用于控制所述核酸分子的表达的调节元件。
10.包含如权利要求7至9中任一项所述的核酸分子的宿主细胞。
11.用于产生如权利要求1至6中任一项所述的多肽的方法，所述方法包括在允许表达所述多肽的条件下培养根据权利要求9所述的宿主细胞，并且从所述宿主细胞或培养基中分离所述多肽。
12.具有环化酶活性的多肽用于使肽环化的用途，具有环化酶活性的所述多肽是如权利要求1至6中任一项所述的分离的多肽或包含以下项或由其组成
(i)如在SEQ ID No:3-109中列出的任一个氨基酸序列；
(ii)在其整个长度上与(i)的任一个氨基酸序列具有至少60％、优选至少70％、更优选至少80％、最优选至少90％序列同一性的氨基酸序列；
(iii)在其整个长度上与(i)的任一个氨基酸序列具有至少80％、优选至少90％、更优选至少95％序列同源性的氨基酸序列；或
(iv)(i)-(iii)中任一项的片段。
13.如权利要求12所述的用途，其中待环化的所述肽包含
(i)氨基酸序列(X)oN/D(X)p，优选地在C-末端，其中X是任何氨基酸并且o和p彼此独立地是至少2的整数，优选氨基酸序列(X)oNHV；或
(ii)C-末端氨基酸序列(X)oN*/D*，其中X是任何氨基酸，o是至少2的整数，并且所述C-末端N/D残基被酰胺化，具体地说，C-末端羧基基团、在D的情况下优选α-羧基基团，被具有化学式–C(O)-N(R’)2的酰胺基置换，其中R’是任何残基。
14.如权利要求12或13所述的用途，其中待环化的所述肽包含N-末端氨基酸序列X1X2(X)q，其中X可以是任何氨基酸；X1可以是除Pro外的任何氨基酸；X2可以是任何氨基酸，但是优选地是疏水性氨基酸，更优选Val、Ile或Leu；并且q是0或者1或更大的整数。
15.如权利要求12至14中任一项所述的用途，其中待环化的所述肽是环状胱氨酸结多肽、环肽毒素、环状抗微生物肽、环状组胺素、或人类或动物环肽激素的线性前体形式。
16.如权利要求12至15中任一项所述的用途，其中待环化的所述肽的长度是10个或更多个氨基酸。
17.如权利要求12至16中任一项所述的用途，其中待环化的所述肽包含以下项或由其组成
(i)在SEQ ID No:110-116和128-132的任一项中列出的氨基酸；或
(ii)氨基酸序列(X)nC(X)nC(X)nC(X)nC(X)nC(X)nC(X)nNHV(X)n,，其中每个n是独立地选自1至6的整数并且X可以是任何氨基酸。
18.具有蛋白质连接酶活性的多肽用于连接至少两种肽的用途，具有蛋白质连接酶活性的所述多肽是如权利要求1至6中任一项所述的分离的多肽或包含以下项或由其组成
(i)如在SEQ ID No:3-109中列出的任一个氨基酸序列；
(ii)在其整个长度上与(i)的任一个氨基酸序列具有至少60％、优选至少70％、更优选至少80％、最优选至少90％序列同一性的氨基酸序列；
(iii)在其整个长度上与(i)的任一个氨基酸序列具有至少80％、优选至少90％、更优选至少95％序列同源性的氨基酸序列；或
(iv)(i)-(iii)中任一项的片段。
19.如权利要求18所述的用途，其中待连接的所述肽中的至少一种包含可检测标记物，优选荧光标记物或生物素。
20.如权利要求18或19所述的用途，其中待连接的所述肽中的至少一种包含
(i)氨基酸序列(X)oN/D(X)p，优选地在C-末端，其中X是任何氨基酸并且o和p彼此独立地是至少2的整数，优选氨基酸序列(X)oNHV；或
(ii)C-末端氨基酸序列(X)oN*/D*，其中X是任何氨基酸，o是至少2的整数并且所述C-末端N/D残基被酰胺化，具体地说，C-末端羧基基团、在D的情况下优选α-羧基基团被具有化学式–C(O)-N(R’)2的酰胺基置换，其中R’是任何残基。
21.如权利要求18至20中任一项所述的用途，其中待连接的所述肽中的至少一种包含N-末端氨基酸序列X1X2(X)q，其中X可以是任何氨基酸；X1可以是除Pro外的任何氨基酸；X2可以是任何氨基酸，但是优选地是疏水性氨基酸，更优选Val、Ile或Leu；并且q是0或者1或更大的整数。
22.如权利要求18至21中任一项所述的用途，其中待连接的所述肽中的至少一种的长度是25个或更多个、优选50个或更多个氨基酸。
23.用于使肽环化的方法，所述方法包括在允许环化所述肽的条件下用如权利要求1至6中任一项所述的分离的多肽或包含以下项或由其组成的多肽孵育所述肽
(i)如在SEQ ID No:3-109中列出的任一个氨基酸序列；
(ii)在其整个长度上与(i)的任一个氨基酸序列具有至少60％、优选至少70％、更优选至少80％、最优选至少90％序列同一性的氨基酸序列；
(iii)在其整个长度上与(i)的任一个氨基酸序列具有至少80％、优选至少90％、更优选至少95％序列同源性的氨基酸序列；或
(iv)(i)-(iii)中任一项的片段。
24.用于连接至少两种肽的方法，所述方法包括在允许连接所述肽的条件下用如权利要求1至6中任一项所述的分离的多肽或包含以下项或由其组成的多肽孵育所述至少两种肽
(i)如在SEQ ID No:3-109中列出的任一个氨基酸序列；
(ii)在其整个长度上与(i)的任一个氨基酸序列具有至少60％、优选至少70％、更优选至少80％、最优选至少90％序列同一性的氨基酸序列；
(iii)在其整个长度上与(i)的任一个氨基酸序列具有至少80％、优选至少90％、更优选至少95％序列同源性的氨基酸序列；或
(iv)(i)-(iii)中任一项的片段。
25.如权利要求23或24所述的方法，其中待环化的所述肽或待连接的所述至少一种肽包含
(i)氨基酸序列(X)oN/D(X)p，优选在C-末端，其中X是任何氨基酸并且o和p彼此独立地是至少2的整数，优选氨基酸序列(X)oNHV；或
(ii)C-末端氨基酸序列(X)oN*/D*，其中X是任何氨基酸，o是至少2的整数并且所述C-末端N/D残基被酰胺化，具体地说，C-末端羧基基团、在D的情况下优选α-羧基基团被具有化学式–C(O)-N(R’)2的酰胺基置换，其中R’是任何残基。
26.如权利要求25所述的方法，其中待环化的所述肽或待连接的所述至少一种肽是N-末端地融合到氨基酸序列N/D(X)p、优选NHV上的感兴趣的肽的人工融合肽。
27.如权利要求23至26中任一项所述的方法，其中待环化的所述肽或待连接的所述至少一种肽包含N-末端氨基酸序列X1X2(X)q，其中X可以是任何氨基酸；X1可以是除Pro外的任何氨基酸；X2可以是任何氨基酸，但是优选地是疏水性氨基酸，更优选Val、Ile或Leu；并且q是0或者1或更大的整数。
28.包含如权利要求7至9中任一项所述的核酸的转基因植物。
29.如权利要求28所述的转基因植物，进一步包含至少一种核酸分子，所述核酸分子编码待环化的一种或多种肽或待连接的一种或多种肽，优选环状胱氨酸结多肽、环肽毒素、环状抗微生物肽、环状组胺素、或人类或动物环肽激素的线性前体形式。
30.包含固定在其上的根据权利要求1-6中任一项所述的分离的多肽的固体支持材料。
31.如权利要求30所述的固体支持材料，其中所述固体支持材料包含聚合物树脂，优选处于微粒形式。
32.如权利要求30或31所述的固体支持材料，其中所述分离的多肽通过共价或非共价相互作用固定在所述固体支持材料上。
33.如权利要求32所述的固体支持材料，其中所述分离的多肽被非共价地结合到偶联在所述固体支持材料表面上的碳水化合物结合部分、优选伴刀豆球蛋白A上。
34.如权利要求30至33中任一项所述的固体支持材料，其中所述固体支持材料是用于层析柱的微粒树脂材料。
35.如权利要求34所述的固体支持材料用于柱上环化和/或连接至少一种底物肽的用途。
36.用于环化或连接至少一种底物肽的方法，所述方法包括使包含所述至少一种底物肽的溶液与如权利要求30至34中任一项所述的固体支持材料在允许环化和/或连接所述至少一种底物肽的条件下接触。
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CN113811606A (zh) * 2019-05-07 2021-12-17 南洋理工大学 Asx特异性蛋白质连接酶及其用途
CN111117977A (zh) * 2020-01-06 2020-05-08 华南理工大学 一种重组多肽连接酶原及其制备、激活方法与应用
WO2021139295A1 (zh) * 2020-01-06 2021-07-15 华南理工大学 一种重组多肽连接酶原及其制备、激活方法与应用
CN111117977B (zh) * 2020-01-06 2021-09-10 广州英赞生物科技有限公司 一种重组多肽连接酶原及其制备、激活方法与应用
CN114854782A (zh) * 2022-05-12 2022-08-05 华南理工大学 一种高效表达具有高活性的重组多肽连接酶原的方法
CN114854782B (zh) * 2022-05-12 2023-06-20 华南理工大学 一种高效表达具有高活性的重组多肽连接酶原的方法
CN117169393A (zh) * 2023-11-03 2023-12-05 杭州湃肽生化科技有限公司 一种植物组织中环肽的检测方法
CN117169393B (zh) * 2023-11-03 2024-03-19 杭州湃肽生化科技有限公司 一种植物组织中环肽的检测方法

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US20170044515A1 (en) 2017-02-16
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