CN106442443A - 一种微液滴荧光检测系统 - Google Patents
一种微液滴荧光检测系统 Download PDFInfo
- Publication number
- CN106442443A CN106442443A CN201610818495.8A CN201610818495A CN106442443A CN 106442443 A CN106442443 A CN 106442443A CN 201610818495 A CN201610818495 A CN 201610818495A CN 106442443 A CN106442443 A CN 106442443A
- Authority
- CN
- China
- Prior art keywords
- chip
- microlayer model
- fluorescence
- detection
- drop
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 claims abstract description 102
- 238000000034 method Methods 0.000 claims abstract description 21
- 230000008569 process Effects 0.000 claims abstract description 6
- 230000005284 excitation Effects 0.000 claims description 28
- 238000003384 imaging method Methods 0.000 claims description 16
- 230000003287 optical effect Effects 0.000 claims description 11
- 239000008346 aqueous phase Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000012071 phase Substances 0.000 claims description 4
- 230000005611 electricity Effects 0.000 claims description 3
- 230000000630 rising effect Effects 0.000 claims description 3
- 238000002189 fluorescence spectrum Methods 0.000 claims description 2
- 230000004907 flux Effects 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 230000003068 static effect Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 238000005286 illumination Methods 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 238000007689 inspection Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000004087 circulation Effects 0.000 description 2
- 238000007847 digital PCR Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 101150034593 Gjb2 gene Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 230000005622 photoelectricity Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1484—Electro-optical investigation, e.g. flow cytometers microstructural devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0803—Disc shape
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0883—Serpentine channels
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8405—Application to two-phase or mixed materials, e.g. gas dissolved in liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/07—Centrifugal type cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
Abstract
Description
Claims (10)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610818495.8A CN106442443B (zh) | 2016-09-12 | 2016-09-12 | 一种微液滴荧光检测系统 |
PCT/CN2017/100658 WO2018045953A1 (zh) | 2016-09-12 | 2017-09-06 | 一种微液滴荧光检测系统 |
US16/328,990 US10724957B2 (en) | 2016-09-12 | 2017-09-06 | Micro-droplet fluorescence detection system |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610818495.8A CN106442443B (zh) | 2016-09-12 | 2016-09-12 | 一种微液滴荧光检测系统 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106442443A true CN106442443A (zh) | 2017-02-22 |
CN106442443B CN106442443B (zh) | 2018-12-07 |
Family
ID=58168693
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610818495.8A Active CN106442443B (zh) | 2016-09-12 | 2016-09-12 | 一种微液滴荧光检测系统 |
Country Status (3)
Country | Link |
---|---|
US (1) | US10724957B2 (zh) |
CN (1) | CN106442443B (zh) |
WO (1) | WO2018045953A1 (zh) |
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107748138A (zh) * | 2017-12-05 | 2018-03-02 | 山东交通学院 | 基于滤光液原理的微藻活性检测装置与方法 |
WO2018045953A1 (zh) * | 2016-09-12 | 2018-03-15 | 北京天健惠康生物科技有限公司 | 一种微液滴荧光检测系统 |
CN108333115A (zh) * | 2018-03-01 | 2018-07-27 | 北京天健惠康生物科技有限公司 | 一种微液滴检测装置 |
CN108414446A (zh) * | 2018-03-30 | 2018-08-17 | 广东顺德墨赛生物科技有限公司 | 微流控芯片荧光检测设备、方法以及装置 |
CN108414773A (zh) * | 2018-07-12 | 2018-08-17 | 上海速创诊断产品有限公司 | 一种微流控检测芯片及其制备方法、固定装置和离心式检测装置 |
CN108844936A (zh) * | 2018-07-09 | 2018-11-20 | 领航基因科技(杭州)有限公司 | 一种三维液滴检测系统及检测方法 |
CN109746063A (zh) * | 2017-11-06 | 2019-05-14 | 北京新羿生物科技有限公司 | 微液滴检测系统 |
CN109999933A (zh) * | 2019-04-26 | 2019-07-12 | 东莞东阳光医疗智能器件研发有限公司 | 一种离心式液滴发生装置 |
CN110331088A (zh) * | 2019-08-17 | 2019-10-15 | 新羿制造科技(北京)有限公司 | 微液滴荧光检测系统与方法 |
CN110358676A (zh) * | 2019-08-17 | 2019-10-22 | 清华大学 | 数字pcr成像法检测中应用的微液滴取样装置 |
WO2019233245A1 (zh) * | 2018-06-07 | 2019-12-12 | 洛阳华清天木生物科技有限公司 | 一种微液滴处理装置及其使用方法 |
WO2020056889A1 (zh) * | 2018-09-20 | 2020-03-26 | 清华大学 | 一种检测混合体系内各成分含量的方法及装置 |
CN111157508A (zh) * | 2020-03-06 | 2020-05-15 | 成都博奥晶芯生物科技有限公司 | 一种微流体芯片荧光数据连续采集方法 |
WO2020119562A1 (zh) * | 2018-12-15 | 2020-06-18 | 中国科学院深圳先进技术研究院 | 多色荧光同步检测的液滴微流控芯片 |
CN112295619A (zh) * | 2020-10-06 | 2021-02-02 | 新羿制造科技(北京)有限公司 | 微液滴芯片沟道定位装置及方法 |
CN112795989A (zh) * | 2021-04-07 | 2021-05-14 | 季华实验室 | 微滴式数字聚合酶链式反应芯片 |
CN113167725A (zh) * | 2019-11-22 | 2021-07-23 | 京东方科技集团股份有限公司 | 分析仪及检测系统 |
CN113956968A (zh) * | 2021-10-20 | 2022-01-21 | 西安天隆科技有限公司 | 一种液滴型数字化pcr系统及其实现绝对定量化的分析方法 |
CN114112826A (zh) * | 2021-11-25 | 2022-03-01 | 天津大学 | 用于荧光微粒的声光互联微流控检测系统和检测方法 |
CN109999933B (zh) * | 2019-04-26 | 2024-04-26 | 东莞东阳光医疗智能器件研发有限公司 | 一种离心式液滴发生装置 |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110487725A (zh) * | 2019-09-04 | 2019-11-22 | 新羿制造科技(北京)有限公司 | 能够自动聚焦的检测装置及相应的自动聚焦方法 |
CN111974470A (zh) * | 2020-06-09 | 2020-11-24 | 天津城建大学 | 一种在异质衬底上实现微液滴输运的装置及方法 |
CN113980796B (zh) * | 2021-10-25 | 2023-12-26 | 苏州缔因安生物科技有限公司 | 一种微液滴承载芯片、其用途、核酸检测模块及方法 |
CN114994007A (zh) * | 2022-08-04 | 2022-09-02 | 长春理工大学 | 数字pcr检测系统 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2010081838A (ja) * | 2008-09-30 | 2010-04-15 | Nsk Ltd | マイクロ流体チップおよび細胞計測装置 |
US20120196288A1 (en) * | 2011-01-27 | 2012-08-02 | Lawrence Livermore National Security, Llc | Chip-Based Droplet Sorting |
CN102879366A (zh) * | 2012-09-21 | 2013-01-16 | 常州大学 | 检测量子点与生物分子相互作用的液滴微流控系统及方法 |
CN104007091A (zh) * | 2013-02-26 | 2014-08-27 | 中国科学院天津工业生物技术研究所 | 一种基于液滴微流控芯片的微生物高通量检测系统 |
CN204474676U (zh) * | 2015-01-30 | 2015-07-15 | 中国科学院苏州生物医学工程技术研究所 | 一种基于微滴实验的微流控检测芯片 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999014368A2 (en) * | 1997-09-15 | 1999-03-25 | Whitehead Institute For Biomedical Research | Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device |
US20050264805A1 (en) * | 2004-02-09 | 2005-12-01 | Blueshift Biotechnologies, Inc. | Methods and apparatus for scanning small sample volumes |
TWI266053B (en) | 2004-02-23 | 2006-11-11 | Nanya Inst Of Technology | Method for testing portable centrifugal microfluidic chip and its device |
CN101620187A (zh) | 2008-07-04 | 2010-01-06 | 中国科学院大连化学物理研究所 | 一种高灵敏度的旋转扫描式多通道化学发光检测仪 |
EP2239557B1 (de) | 2009-04-09 | 2012-01-25 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren zur Messung luftgetragener biologischer Gefahrstoffe |
CN104736725A (zh) * | 2012-08-13 | 2015-06-24 | 加利福尼亚大学董事会 | 用于检测生物组分的方法和系统 |
EP3017868A1 (en) * | 2014-11-10 | 2016-05-11 | Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie - Hans-Knöll-Institut - | Device and method for extracting individual picoliter droplets from microfluidic emulsions for further analysis and scale-up |
CN105300943B (zh) | 2015-11-03 | 2019-04-26 | 中国科学院天津工业生物技术研究所 | 一种用于液滴荧光检测的显微镜集成光路系统 |
CN106442443B (zh) | 2016-09-12 | 2018-12-07 | 北京天健惠康生物科技有限公司 | 一种微液滴荧光检测系统 |
-
2016
- 2016-09-12 CN CN201610818495.8A patent/CN106442443B/zh active Active
-
2017
- 2017-09-06 US US16/328,990 patent/US10724957B2/en active Active
- 2017-09-06 WO PCT/CN2017/100658 patent/WO2018045953A1/zh active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2010081838A (ja) * | 2008-09-30 | 2010-04-15 | Nsk Ltd | マイクロ流体チップおよび細胞計測装置 |
US20120196288A1 (en) * | 2011-01-27 | 2012-08-02 | Lawrence Livermore National Security, Llc | Chip-Based Droplet Sorting |
CN102879366A (zh) * | 2012-09-21 | 2013-01-16 | 常州大学 | 检测量子点与生物分子相互作用的液滴微流控系统及方法 |
CN104007091A (zh) * | 2013-02-26 | 2014-08-27 | 中国科学院天津工业生物技术研究所 | 一种基于液滴微流控芯片的微生物高通量检测系统 |
CN204474676U (zh) * | 2015-01-30 | 2015-07-15 | 中国科学院苏州生物医学工程技术研究所 | 一种基于微滴实验的微流控检测芯片 |
Non-Patent Citations (3)
Title |
---|
JEAN-CHRISTOPHE ROULET ET AL: "Performance of an Integrated Microoptical System for Fluorescence Detection in Microfluidic Systems", 《ANAL. CHEM》 * |
JISEOK LIM ET AL: "Micro-optical lens array for fluorescence detection in droplet-based microfluidics", 《LAB ON A CHIP》 * |
张凯 等: "微流控芯片中微液滴的操控及其应用", 《分析化学》 * |
Cited By (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2018045953A1 (zh) * | 2016-09-12 | 2018-03-15 | 北京天健惠康生物科技有限公司 | 一种微液滴荧光检测系统 |
US10724957B2 (en) | 2016-09-12 | 2020-07-28 | Targetingone Corporation | Micro-droplet fluorescence detection system |
CN109746063A (zh) * | 2017-11-06 | 2019-05-14 | 北京新羿生物科技有限公司 | 微液滴检测系统 |
CN107748138B (zh) * | 2017-12-05 | 2023-11-28 | 山东交通学院 | 基于滤光液原理的微藻活性检测装置与方法 |
CN107748138A (zh) * | 2017-12-05 | 2018-03-02 | 山东交通学院 | 基于滤光液原理的微藻活性检测装置与方法 |
CN108333115A (zh) * | 2018-03-01 | 2018-07-27 | 北京天健惠康生物科技有限公司 | 一种微液滴检测装置 |
CN108414446A (zh) * | 2018-03-30 | 2018-08-17 | 广东顺德墨赛生物科技有限公司 | 微流控芯片荧光检测设备、方法以及装置 |
WO2019233245A1 (zh) * | 2018-06-07 | 2019-12-12 | 洛阳华清天木生物科技有限公司 | 一种微液滴处理装置及其使用方法 |
CN108844936A (zh) * | 2018-07-09 | 2018-11-20 | 领航基因科技(杭州)有限公司 | 一种三维液滴检测系统及检测方法 |
CN108414773A (zh) * | 2018-07-12 | 2018-08-17 | 上海速创诊断产品有限公司 | 一种微流控检测芯片及其制备方法、固定装置和离心式检测装置 |
CN110923296B (zh) * | 2018-09-20 | 2021-01-26 | 清华大学 | 一种检测混合体系内各成分含量的方法及装置 |
WO2020056889A1 (zh) * | 2018-09-20 | 2020-03-26 | 清华大学 | 一种检测混合体系内各成分含量的方法及装置 |
CN110923296A (zh) * | 2018-09-20 | 2020-03-27 | 清华大学 | 一种检测混合体系内各成分含量的方法及装置 |
WO2020119562A1 (zh) * | 2018-12-15 | 2020-06-18 | 中国科学院深圳先进技术研究院 | 多色荧光同步检测的液滴微流控芯片 |
CN109999933A (zh) * | 2019-04-26 | 2019-07-12 | 东莞东阳光医疗智能器件研发有限公司 | 一种离心式液滴发生装置 |
CN109999933B (zh) * | 2019-04-26 | 2024-04-26 | 东莞东阳光医疗智能器件研发有限公司 | 一种离心式液滴发生装置 |
CN110358676A (zh) * | 2019-08-17 | 2019-10-22 | 清华大学 | 数字pcr成像法检测中应用的微液滴取样装置 |
CN110331088A (zh) * | 2019-08-17 | 2019-10-15 | 新羿制造科技(北京)有限公司 | 微液滴荧光检测系统与方法 |
CN110331088B (zh) * | 2019-08-17 | 2023-09-22 | 新羿制造科技(北京)有限公司 | 微液滴荧光检测系统与方法 |
CN113167725A (zh) * | 2019-11-22 | 2021-07-23 | 京东方科技集团股份有限公司 | 分析仪及检测系统 |
CN111157508B (zh) * | 2020-03-06 | 2022-09-06 | 成都博奥晶芯生物科技有限公司 | 一种微流体芯片荧光数据连续采集方法 |
CN111157508A (zh) * | 2020-03-06 | 2020-05-15 | 成都博奥晶芯生物科技有限公司 | 一种微流体芯片荧光数据连续采集方法 |
CN112295619B (zh) * | 2020-10-06 | 2022-01-18 | 新羿制造科技(北京)有限公司 | 微液滴芯片沟道定位装置及方法 |
CN112295619A (zh) * | 2020-10-06 | 2021-02-02 | 新羿制造科技(北京)有限公司 | 微液滴芯片沟道定位装置及方法 |
CN112795989B (zh) * | 2021-04-07 | 2021-07-06 | 季华实验室 | 微滴式数字聚合酶链式反应芯片 |
CN112795989A (zh) * | 2021-04-07 | 2021-05-14 | 季华实验室 | 微滴式数字聚合酶链式反应芯片 |
CN113956968A (zh) * | 2021-10-20 | 2022-01-21 | 西安天隆科技有限公司 | 一种液滴型数字化pcr系统及其实现绝对定量化的分析方法 |
CN113956968B (zh) * | 2021-10-20 | 2023-11-14 | 西安天隆科技有限公司 | 一种液滴型数字化pcr系统及其实现绝对定量化的分析方法 |
CN114112826A (zh) * | 2021-11-25 | 2022-03-01 | 天津大学 | 用于荧光微粒的声光互联微流控检测系统和检测方法 |
CN114112826B (zh) * | 2021-11-25 | 2024-04-19 | 天津大学 | 用于荧光微粒的声光互联微流控检测系统和检测方法 |
Also Published As
Publication number | Publication date |
---|---|
CN106442443B (zh) | 2018-12-07 |
US10724957B2 (en) | 2020-07-28 |
US20190212264A1 (en) | 2019-07-11 |
WO2018045953A1 (zh) | 2018-03-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106442443A (zh) | 一种微液滴荧光检测系统 | |
CN101576557B (zh) | 一种集成微流控芯片系统 | |
JP3429282B2 (ja) | 自動化されたシステム、及びサンプルの分析方法 | |
CN103901189B (zh) | 免疫检测微流控芯片荧光定量自动检测装置 | |
CN108414446A (zh) | 微流控芯片荧光检测设备、方法以及装置 | |
CN108444897B (zh) | 基于光片照明与鞘流技术的免标记微流控细胞仪及方法 | |
JP2010524004A (ja) | 微粒子を分析するためのシステムおよび方法 | |
CN102879366B (zh) | 检测量子点与生物分子相互作用的液滴微流控系统及方法 | |
CN103675053A (zh) | 一种基于表面等离子体谐振的局部电化学成像测试系统 | |
CN108426886B (zh) | 一种循环肿瘤细胞的检测识别方法和系统 | |
CN106525803A (zh) | 生物成像仪 | |
King et al. | Optical detection strategies for centrifugal microfluidic platforms | |
CN109632721A (zh) | 一种lrspr-荧光成像并行检测装置及lrspr芯片制作方法 | |
JP5703098B2 (ja) | 生体分子検出装置および生体分子検出方法 | |
CN104749162A (zh) | 共聚焦拉曼光谱仪及其光路装置 | |
JP2001521167A (ja) | 蛍光免疫分析を行う装置および方法 | |
CN103063645A (zh) | 基于新型微结构光纤的高效荧光检测 | |
CN104749105A (zh) | 基于近红外光镊激发上转换发光的定量检测装置及检测方法 | |
CN108106994A (zh) | 一种扫描式局域增强生化传感装置 | |
US20240027325A1 (en) | Method and apparatus for flow-based, single-particle and/or single-molecule analysis | |
US20210302300A1 (en) | Serial flow cytometer | |
CN103033464A (zh) | 光声-荧光流式细胞仪 | |
CN112525884A (zh) | 一种超微量拉曼光谱检测装置 | |
CN108802008A (zh) | 一种利用受激拉曼光谱检测血液中肿瘤细胞的方法和装置 | |
CN105388131A (zh) | 基于微流控芯片的荧光检测仪器及系统 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20170630 Address after: 100190 Beijing, Haidian District, Zhongguancun, north two, No. 44, building 310, room 13 Applicant after: Beijing Tianjian Wellcome Biotechnology Co. Ltd. Address before: 100084 Beijing, Beijing, Haidian, Haidian District, Tsinghua Yuan Applicant before: Tsinghua University Applicant before: Beijing Tianjian Wellcome Biotechnology Co. Ltd. |
|
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20170907 Address after: 100084 Beijing City, Haidian District Tsinghua Yuan Applicant after: Tsinghua University Applicant after: Beijing Tianjian Wellcome Biotechnology Co. Ltd. Address before: 100190 Beijing, Haidian District, Zhongguancun, north two, No. 44, building 310, room 13 Applicant before: Beijing Tianjian Wellcome Biotechnology Co. Ltd. |
|
CB03 | Change of inventor or designer information |
Inventor after: Su Shisheng Inventor after: Wang Yongdou Inventor after: Guo Yong Inventor after: Jing Gaoshan Inventor after: Wang Bo Inventor after: Zhang Miaoqi Inventor after: Liu Zhe Inventor after: Gao Huafang Inventor after: Zhu Lingxiang Inventor after: Yang Wenjun Inventor before: Su Shisheng Inventor before: Wang Yongdou Inventor before: Guo Yong Inventor before: Jing Gaoshan Inventor before: Wang Bo Inventor before: Zhang Miaoqi Inventor before: Liu Zhe Inventor before: Gao Huafang Inventor before: Zhu Lingxiang Inventor before: Yang Wenjun |
|
CB03 | Change of inventor or designer information | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20180115 Address after: 100190 Beijing, Haidian District, Zhongguancun, north two, No. 44, building 310, room 13 Applicant after: Beijing Tianjian Wellcome Biotechnology Co. Ltd. Address before: 100084 Beijing City, Haidian District Tsinghua Yuan Applicant before: Tsinghua University Applicant before: Beijing Tianjian Wellcome Biotechnology Co. Ltd. |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20181130 Address after: Room 101, Building 8, No. 1 Qianqian Road, Changping District Science and Technology Park, Beijing 102200 Patentee after: The new Yi Technology (Beijing) Co. Ltd., manufacturing Address before: Room 310, 44 Block No. 13, North Second Article, Zhongguancun, Haidian District, Beijing, 100190 Patentee before: Beijing Tianjian Wellcome Biotechnology Co. Ltd. |