CN106434623A - Method for controlling growth of escherichia coli by means of surface acoustic wave technology - Google Patents
Method for controlling growth of escherichia coli by means of surface acoustic wave technology Download PDFInfo
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- CN106434623A CN106434623A CN201610725085.9A CN201610725085A CN106434623A CN 106434623 A CN106434623 A CN 106434623A CN 201610725085 A CN201610725085 A CN 201610725085A CN 106434623 A CN106434623 A CN 106434623A
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- saw
- escherichia coli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- H—ELECTRICITY
- H03—ELECTRONIC CIRCUITRY
- H03H—IMPEDANCE NETWORKS, e.g. RESONANT CIRCUITS; RESONATORS
- H03H9/00—Networks comprising electromechanical or electro-acoustic devices; Electromechanical resonators
- H03H9/25—Constructional features of resonators using surface acoustic waves
Abstract
The invention discloses a method for controlling growth of escherichia coli by means of surface acoustic wave (SAW) technology, belongs to the technical field of novel electronic devices and biology, and particularly relates to a method for promoting growth of escherichia coli by means of high-frequency low-energy (wherein the frequency is not larger than 1 MHz and energy is 0.1-3 W) surface acoustic waves and restraining the growth of escherichia coli and even killing escherichia coli by means of high-frequency high-energy (energy is larger than 5 W) surface acoustic wave to achieve effective control over the growth of escherichia coli. An SAW device is prepared on a piezoelectric material, and an input radio-frequency signal is converted into an SAW signal through an interdigital transducer (IDT) and broadcast towards a PDMS container containing escherichia coli and a nutrient solution; after the SAW is broadcast to a PDMS container, SAW energy is coupled to the nutrient solution containing escherichia coli as shown in the figure 1. The method for controlling growth of escherichia coli has the advantages that equipment is simple, the technology is simple and convenient, growth control over the escherichia coli in a local area can be achieved, and the wide application prospect is achieved.
Description
Technical field
The present invention relates to a kind of utilize surface acoustic wave (surface acoustic wave, SAW) technical controlling Escherichia coli
The method of growth, specifically utilizes the low-yield surface acoustic wave of high frequency to promote colibacillary growth, on the other hand utilizes height
The colibacillary growth of frequency high-energy surface acoustic wave suppression even kills Escherichia coli, realizes effective control of Escherichia coli Growth
System, belongs to Novel electronic devices and biological technical field.
Background technology
Escherichia coli are a kind of most commonly seen bacteriums in the big enteral of humans and animals for the main parasitic, account for enteric bacteria
1%.Escherichia coli are a kind of unicellular organisms, and its structure is simple, and breeding is fast, and culture easily, is biologically important experiment
Material.In addition to some bacterial types can cause diarrhoea, the Escherichia coli settled down in enteron aisle are typically not pathogenic, and resisted by competitiveness
The attack of pathogenic bacteria, can also help synthetic vitamin B and K simultaneously, beneficial to human body, be the relation of mutualism with human body.
Under normal circumstances, most of Escherichia coli will not bring any harm to our healthy.But some are special
In the case of different, Escherichia coli also result in disease：As urinary tract infection can be led to when Escherichia coli leave enteron aisle entrance the urinary tract；
When bacterium because the perforation that such as ulcer etc. leads to enters abdominal cavity it will usually lead to fatefulue peritonitis infection；Colibacillary
Some strains have toxicity (some of them are similar to the toxin leading to dysentery), can lead to poison by food.At this time generally require and adopt
With antibiotic, Escherichia coli are killed.
Therefore control growth extremely important for colibacillary.In the area wishing Escherichia coli fast-growth and breeding
Domain, can effectively facilitate colibacillary growth, be not intended to Escherichia coli Growth and the region of breeding at some, need it is entered
Row is effectively killed.Industry has carried out numerous studies to Escherichia coli culture, finds that impact Escherichia coli cultivate the factor of bacteria concentration
Have a lot, such as culture medium composition, incubation time, bacterial classification inoculum concentration and environment of bacteria growth etc..The method of these researchs is all no
Method is realized regional area and is controlled colibacillary growth, realizes to Escherichia coli Growth simultaneously nor using a kind of technology simultaneously
Promote and kill.
Content of the invention
The present invention discloses a kind of method that utilization surface acoustic wave techniques controls Escherichia coli Growth.The method one side utilizes
The low-yield surface acoustic wave of high frequency promotes colibacillary growth, on the other hand utilizes high frequency high-energy surface acoustic wave to suppress large intestine bar
The growth of bacterium even kills Escherichia coli, realizes the effective control of Escherichia coli Growth.The method device therefor is simple, technique letter
Just, the Escherichia coli Growth being capable of regional area controls.
Concrete technical scheme of the present invention is as follows：
Using SAW technical controlling Escherichia coli Growth device as shown in figure 1, include substrate on piezoelectric, piezoelectricity
Interdigital transducer (interdigital transducer, IDT) for exciting SAW is provided with material and is used for reflecting
The reflecting grating of SAW, for accommodating the PDMS container of Escherichia coli and nutrient solution.After the input radio frequency signal of IDT two ends, radio frequency is believed
Number by IDT be converted to SAW signal and to PDMS container direction propagate, when SAW propagate reach PDMS container after, SAW energy coupling
Close to enter and contain colibacillary nutrient solution, by controlling SAW energy and the disturbance to nutrient solution being coupled into nutrient solution
Situation, realizes the effective control to Escherichia coli Growth.
With regard to technical solution of the present invention it should be noted that：
Hydrophobic layer is coated with SAW propagation path, PDMS container is the container of open top and bottom opening, and bottom is close to
It is connected on hydrophobic layer.IDT interdigital transducer can adopt the metallic films such as Al and Au to make.Piezoelectric and backing material can
Think piezoelectric monocrystal (quartz, lithium niobate, lithium tantalate etc.) or piezoelectric film material (ZnO, AlN, PZT).Radiofrequency signal
Incoming frequency and input power all can be adjusted, and input different frequency and power radiofrequency signal to colibacillary growth
Impact is different, and by adjusting this two important parameters, and the different SAW patterns of output just can control colibacillary life
Long.
It is an advantage of the invention that：Size due to SAW device can be adjusted, and can accomplish 1mm at present2Hereinafter, therefore may be used
Colibacillary growth is controlled with localized region.Promote colibacillary growth using SAW technology without using biochemistry
Reagent, the physical environment fully relying on outside is adjusted, therefore the pollution of no other chemical reagent.Utilize SAW technology to suppress simultaneously
With killing Escherichia coli it is not necessary to use antibiotic, do not interfere with the growth of other microorganisms of surrounding.Only with SAW technology,
Can realize at short notice promoting and the colibacillary growth of suppression by adjusting SAW parameter.
Brief description
Fig. 1 is the schematic diagram using SAW technical controlling Escherichia coli Growth.
Fig. 2 is input SAW device power is 2W, and absorbance (optical density, OD) and temperature are with SAW process
The change curve of time.
Fig. 3 is input SAW device power is 8W, and OD and temperature are with the change curve of SAW process time.
Specific embodiment
Below by accompanying drawing and example, technical scheme is described in further detail.
Embodiment 1：
Deposited on a si substrate as piezoelectric using ZnO film, by depositing Al film and using photoetching technique system
Standby IDT and reflecting grating, the interdigital width of IDT and finger spacing are 50 microns, and aperture is 10mm.IDT finger number is right, instead for 80
The bar number penetrating grid is right for 100.The size of PDMS container is 10 × 10 × 10mm3, container wall thickness is 1mm.PDMS container is placed
At 2mm before the IDT.The Escherichia coli solution for 0.23 for the OD value is divided into two groups, one group as a control group, is not loaded with SAW
Reason；；Put in PDMS container for another group.The frequency of the radiofrequency signal of input SAW device is the resonant frequency of SAW device
22.4MHz, when input power is 2W, after 10min is processed, OD value is from 0.23 starting is quick as can see from Figure 2
It is raised to 0.73, this is more a lot of than 0.25 increase of control group, and after showing to process by SAW, Escherichia coli quantity sharply increases, when entering
When one step increases SAW process time, OD value increase becomes apparent from.Show that SAW process can actually promote colibacillary growth.
Measurement SAW process after temperature change curve over time as shown in Fig. 2 originally 10min temperature rise to 35 DEG C of left sides from 28 DEG C
The right side, basicly stable then as time increase temperature, this temperature is relatively suitable for colibacillary breeding, and this is one of
The reason SAW promotes Escherichia coli Growth, but be not most important reason.SAW uses high frequency low energy " micro-disturbance ", perturbation
The nutrient content in nutrient solution quickly can be transferred to around colibacillary for dynamic one side, on the other hand being capable of fast transfer
The excreta that metabolism produces, in addition micro-disturbance can also be beneficial for the growth of cell, therefore, it is possible to significantly increase large intestine
The growth of bacillus.
Embodiment 2：
Using same embodiment 1 identical SAW device and PDMS container, PDMS container is positioned at 2mm before IDT.By OD
It is worth the Escherichia coli solution for 0.95 and is divided into two groups, one group as a control group, is not loaded with SAW process；Put into PDMS for another group to hold
In device.The frequency of the radiofrequency signal of input SAW device is resonant frequency 22.4MHz of SAW device, when input power is for 8W,
After 10min is processed, OD value rapidly drops to 0.53 from 0.95 starting as can see from Figure 3, and this is than control group
1.02 significantly reduce, and show that Escherichia coli quantity drastically declines after high energy SAW is processed, when increasing SAW process further
Between when, OD value reduces further, and after 30min is processed, OD value drops to less than 0.1, be control group 1.26 1/10th not
Arrive.Show that high energy SAW process can actually suppress colibacillary growth even to kill Escherichia coli.High energy SAW processes large intestine
Bacillus, on the one hand significantly improves the temperature of solution, and from shown in Fig. 3, maximum temperature alreadys exceed 47 DEG C.Still further aspect, high energy
SAW produces the fierce disturbance of solution, thus affecting the growth of Escherichia coli inner cell, colibacillary therefore, it is possible to significantly inhibit
Growth, or even kill Escherichia coli.
Claims (3)
1. a kind of method using surface acoustic wave (SAW) technical controlling Escherichia coli Growth it is characterised in that：Including on substrate
Piezoelectric, the upper surface of piezoelectric is working surface, piezoelectric working surface is provided with SAW's is interdigital for exciting
Transducer (interdigital transducer, IDT) and the reflecting grating for reflecting SAW.IDT is by input radio frequency signal
Be converted to SAW signal and propagate forward, SAW propagation path is coated with hydrophobic layer, be placed with for holding at 2mm before IDT
Receive the PDMS container of Escherichia coli and nutrient solution.SAW propagates after reaching PDMS container, is coupled into nutrient solution, by controlling coupling
Close the SAW energy entering nutrient solution, thus controlling colibacillary growth.
2. method according to claim 1 is it is characterised in that (frequency is more than 1MHz, energy 0.1W- when high frequency is low-yield
When 3W) SAW is coupled into nutrient solution, colibacillary growth can be significantly increased.
3. method according to claim 1 is it is characterised in that (frequency is more than more than 1MHz, energy when high frequency high-energy
When 5W) SAW is coupled into nutrient solution, suppress colibacillary growth, or even kill Escherichia coli.
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| CN201610725085.9A CN106434623A (en) | 2016-08-18 | 2016-08-18 | Method for controlling growth of escherichia coli by means of surface acoustic wave technology |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107238556A (en) * | 2017-06-14 | 2017-10-10 | 浙江大学 | A kind of cell adherence force measuring instrument and measuring method based on surface acoustic wave |
| WO2019192125A1 (en) * | 2018-04-04 | 2019-10-10 | 深圳大学 | Biosensor based on surface acoustic wave mode and test method thereof |
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| CN101616707A (en) * | 2006-03-29 | 2009-12-30 | 纳微振动技术公司 | Be used for preventing that catheter from forming the acoustic add-on device of biofilm |
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|---|---|---|---|---|
| CN107238556A (en) * | 2017-06-14 | 2017-10-10 | 浙江大学 | A kind of cell adherence force measuring instrument and measuring method based on surface acoustic wave |
| CN107238556B (en) * | 2017-06-14 | 2019-06-07 | 浙江大学 | A kind of cell adherence force measuring instrument and measurement method based on surface acoustic wave |
| WO2019192125A1 (en) * | 2018-04-04 | 2019-10-10 | 深圳大学 | Biosensor based on surface acoustic wave mode and test method thereof |
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Application publication date: 20170222 |
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