CN102443539A - Microorganism cell culture device based on audible sound wave stimulation - Google Patents
Microorganism cell culture device based on audible sound wave stimulation Download PDFInfo
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- CN102443539A CN102443539A CN2012100184256A CN201210018425A CN102443539A CN 102443539 A CN102443539 A CN 102443539A CN 2012100184256 A CN2012100184256 A CN 2012100184256A CN 201210018425 A CN201210018425 A CN 201210018425A CN 102443539 A CN102443539 A CN 102443539A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
Abstract
The invention provides a microorganism cell culture device based on audible sound wave stimulation. The device comprises a main box body, a power assembly and an electronic control box, wherein a signal generation module, a power amplification module and a display control module are arranged in the electronic control box; a top speaker and a bottom speaker are connected with the signal generation module through the power amplification module; the signal generation module is connected with the display control module through a frequency measurement module; a microphone used for measuring sound waves is also arranged in the main box body; and the microphone is connected with the display control module through a sound intensity measurement module. The microorganism cell culture device based on the audible sound wave stimulation has the advantages that the sound intensity, frequency and motor speed are adjustable and displayable; and through selecting proper sound wave stimulation waveform, stimulation frequency and intensity, the control on the growth rate of biological samples such as microorganism cells and the like subjected to stationary culture or suspension culture, or the regulation on the formation speed of products can be realized, and the effect is obvious.
Description
Technical field
The present invention relates to a kind of culture apparatus, is a kind of microorganism cells culture apparatus that stimulates based on audible sound specifically.
Background technology
Sound wave is a kind of important environmental factor, is widespread in nature, and interacts with organism.By the frequency people it is divided into infrasound (infrasound, 10
-4～20 hertz), audible sound (audible sound, 20～2 * 10
4Hertz) and UW (ultrasound, 2 * 10
4～10
12Hertz).As far back as nineteen twenty-seven, Germany scientist Wood and Loomis just begin the UW biological effect is studied, and the sixties in 20th century, the interaction of infrasonic sound and organism was also paid close attention to by people gradually.More and more go deep into extensive with the biological effect of infrasonic sound and the research of mechanism about ultrasonic now, some Ultrasonic Diagnosises, ultrasound treatment and infrasonic sound diagnosis and treatment new technology are constantly by extensively understanding and acceptance of people.Yet about the research of audible sound, the most of energy of people all concentrates on directions such as sounding mechanism, acoustical signal characteristic, sound reception, processing and identification for a long time, pays close attention to less to the biological effect and the mechanism of sound wave in communication process.
Summary of the invention
Technical problem to be solved by this invention provides a kind of biomass cells culture apparatus that stimulates based on audible sound, can be through biomass cells being applied the audible sound of certain frequency, realize pair cell the speed of growth control or product formed the adjusting of speed.
A kind of microorganism cells culture apparatus that stimulates based on audible sound of the present invention; Comprise main box, Power Component and electronic control box; Described Power Component comprises transmission shaft, phonomoter, adjusting screw mandrel and circular hanging scaffold; Transmission shaft vertically is arranged on the central authorities at main box top through drive bearing, and is connected with phonomoter through drive pulley and transmission belt, and the top that transmission shaft passes main box extend in the main box; Regulate screw mandrel and be arranged on the end that transmission shaft is positioned at main box inside, and transmission shaft and the coaxial setting of adjusting screw mandrel; , through the circular hanging scaffold that has been threaded, main box inside is equipped with uv lamp and fluorescent lamp in the bottom of regulating screw mandrel; Each face of main box inwall all is equipped with acoustic panel main box inner top the overhead speaker is installed; The bottom is equipped with the end and puts speaker; In electronic control box, be provided with signal generating module, power amplifier module and display control module; The overhead speaker is put speaker the end of with and is connected with signal generating module through power amplifier module; Signal generating module is connected with display control module through the frequency measurement module, in main box, also is provided with the microphone that is used for measurement of sound, and microphone is measured module through the sound intensity and is connected with display control module.
The operation viewing window is housed on the described main box, and main box one side is equipped with the sliding door handle, and the bottom is equipped with castor;
Described adjusting screw mandrel is provided with two hex-nuts, and two hex-nuts are clipped in the middle circular hanging scaffold and are fixed on and regulate on the screw mandrel.
Described circular hanging scaffold is the circular steel plate of diameter 150mm, thick 2mm, and 6 claw type steel bushings evenly are installed on circular steel plate circumference; 10mm is wide, 2mm is thick by two for the claw type steel bushing, and the long spring steel plate of 100mm combines, and the spring steel plate right-angled intersection also is fixed on the round tray bottom surface with screw, and spring steel plate fixed distance point 20mm place turns up formation 75 degree angles to the direction that deviates from round tray; The top of spring steel plate bends to the point of fixity direction.
Described electronic control box is provided with display panel, uv lamp switch, switch for fluorescent lamp, sound intensity adjusting knob, frequency adjustment knob, motor start stop switch, variable frequency adjustment knob, signal generator switch and audio player interface; Wherein sound intensity adjusting knob is connected with power amplifier module; The frequency adjustment knob is connected with the frequency measurement module, and the variable frequency adjustment knob is connected with phonomoter.
The signal generating module of apparatus of the present invention electronic control box adopts the ICL8038 chip as function generator, and it can produce waveforms such as single-frequency dextrorotation ripple, square wave, choppy sea, and the acoustic signals power amplifier module amplifies the rear drive speaker operation to it; Handle and drive the result of echo to the LED according to ICL8038 function generator square wave output terminal output signal through the frequency measurement of AT89S52 micro-chip then, be adjusted to the frequency of needs through the regulating frequency knob by 74SL164 and 74SL138; And the acoustical signal of putting speaker according to the end that microphone is experienced; Amplify and handle the back by conversion of MAX1032 analog and AT89S52 micro-chip through the UA741 magnifying glass and drive the sound intensity size of echo to the LED by 74SL164 and 74SL138, adjusting sound intensity adjusting knob make the sound intensity reach need intensity of acoustic wave.Can carry out parameters,acoustic during work as required and select and adjust, the frequency setting precision is ± 1 hertz, and sound intensity setting accuracy is at ± 0.1 decibel; The audio player interface that sets on the electronic control box is through an audio conversion switchable various player; The sound wave volume is adjustable in 5～130 decibels of scopes usually; On the variable frequency adjustment knob, be provided with frequency transformer, adopt stepless frequency control technology transferring power, reaction is fast, and rotation speed change is steady, thereby realizes the optimum matching of power train and motor working condition;
This installs the AC power that used power supply is 220V/50Hz, and the casing rear portion is provided with the supply lead of energized, and electronic control box is provided with battery main switch.
When the present invention carries out sonic treatment; Sterilization earlier; Again the microbiological specimens carrier is put into the claw type steel bushing of circular hanging scaffold; Display control module through electronic control box is accurately modulated various processing parameters, lets quantize realization sonic treatment process through synthetic sound signal through power amplifying system driving dynamicloudspeaker.
Beneficial effect
1, the present invention is on the basis of research audible sound to the influence of unicellular organism growth metabolism; Find that the audible sound biological effect has certain window effect characteristic; Show as certain frequency and intensity according to patience characteristic, prove, a kind of microorganism cells culture apparatus that stimulates based on audible sound of the present invention through a large amount of experimental datas; Through choosing suitable sound stimulation waveform, frequency of stimulation and intensity; Can realize leaving standstill cultivation, or the control of the biological sample speeds of growth such as microorganism cells of suspension culture or product formed the adjusting of speed, effect is remarkable.
2, a kind of microorganism cells culture apparatus that stimulates based on audible sound of the present invention; But the sound intensity, frequency and motor speed minute adjustment, and can show that the microorganism cells sample can be according to need just putting or be inverted; Can export the audible sound of waveforms such as 1 hertz of sine wave, square wave, choppy sea in 20 kilohertz range; Device also is furnished with the audio player interface circuit, can play the sound of various formats, thereby expand the sound wave output function of this device greatly; The sound intensity of apparatus of the present invention sound wave that speaker sends and audio frequency are more near actual value, and the tolerance range of experiment is high;
3, a kind of microorganism cells culture apparatus that stimulates based on audible sound of the present invention, reaction is fast, and rotation speed change is steady, and is simple to operate, with low cost, is easy to promote.
Description of drawings
Fig. 1 is based on the microorganism cells culture apparatus front view that audible sound stimulates;
Fig. 2 is based on the microorganism cells culture apparatus sectional view that audible sound stimulates;
Fig. 3 is based on the direct motor drive and the circular hanging scaffold device sectional view of the microorganism cells culture apparatus of audible sound stimulation;
Fig. 4 is based on the microorganism cells culture apparatus circuit module synoptic diagram that audible sound stimulates;
The acoustic signals that Fig. 5 is based on the microorganism cells culture apparatus of audible sound stimulation takes place and the power amplifier module schematic circuit;
Fig. 6 is based on the frequency measurement and the display module schematic circuit of the microorganism cells culture apparatus of audible sound stimulation;
Fig. 7 is based on the intensity of acoustic wave of the microorganism cells culture apparatus of audible sound stimulation and measures and the display module schematic circuit;
Label is among the figure: 1, electronic control box, 2, display panel, 3, the uv lamp switch, 4, switch for fluorescent lamp S4,5, sound intensity adjusting knob, 6, the frequency adjustment knob; 7, motor start stop switch, 8, the variable frequency adjustment knob, 9, the signal generator switch, 10, the audio player interface, 11, viewing window, 12, castor; 13 sliding door handles, 14, transmission shaft, 15, drive bearing, 16, drive pulley, 17, transmission belt; 18, motor drive mechanism, 19, main box, 20, signal generating module, 21 power amplifier modules, 22, display control module; 23, cloth art acoustic panel, 24, regulate screw mandrel, 25, circular hanging scaffold, 26, microphone; 27, claw type steel bushing, 28, the end puts dynamicloudspeaker, 29, the overhead dynamicloudspeaker, 30, uv lamp, 31, fluorescent lamp; 32, battery main switch, 33, the waveform system selector switch, 34, the speaker system selector switch, 35, the frequency measurement module, 36, sound intensity frequency measurement module, 37, the MAX1032 analog, 38, the UA741 magnifying glass.
Specific embodiments
A kind of microorganism cells culture apparatus that stimulates based on audible sound of the present invention; Comprise main box 19, Power Component and electronic control box 1; Described Power Component comprises transmission shaft 14, phonomoter 18, regulates screw mandrel 24 and circular hanging scaffold 25; Transmission shaft 14 vertically is arranged on the central authorities at main box 19 tops through drive bearing 15, and is connected with electronic 18 through transmission belt 16 and transmission 17, and the top that transmission shaft 14 passes main box 19 extend in the main box 19; Regulate screw mandrel 24 and be arranged on the end that transmission shaft 14 is positioned at main box 19 inside, and transmission shaft 14 and the 24 coaxial settings of adjusting screw mandrel; Through the circular hanging scaffold 25 that has been threaded, main box 19 inside are equipped with uv lamp 30 and fluorescent lamp 31 in the bottom of regulating screw mandrel 24; Each face of main box 19 inwalls all is equipped with cloth art acoustic panel 23; Its sound absorption frequency spectrum is high; High, medium and low noise all there is preferable acoustically effective, has excellent sound absorbing capabilities, both can isolate extraneous sound field; Thereby can reduce inner acoustic reflection again and eliminate higher harmonic or waveform distortion that device internal reflection sound wave produces because of factors such as stacks; Significantly reduce the experimental error of bringing owing to the influence of other sound wave, made the sound intensity that micro-biological samples experiences and audio frequency more approach the sound intensity and the audio frequency actual value of sound wave that speaker sends in the device, improved the tolerance range of testing; Main box 19 inner tops are equipped with overhead speaker 28; The bottom is equipped with the end and puts speaker 29, can select the use overhead speaker or the end put speaker according to experiment needs, and have water-proof function; When carrying out the deep liquid cultivation, speaker can put in and carry out the sound stimulation processing in the nutrient solution; In electronic control box 1, be provided with signal generating module 20, power amplifier module 2 and display control module 22; Overhead speaker 28 is put speaker 29 end of with and is connected with signal generating module 20 through power amplifier module 21; Signal generating module 20 is connected with display control module 22 through frequency measurement module 35; In main box 19, also be provided with the microphone 26 that is used for measurement of sound, microphone 26 is measured module 36 through the sound intensity and is connected with display control module 22.
Described electronic control box 1 is provided with display panel 2, uv lamp switch 3, switch for fluorescent lamp 4, sound intensity adjusting knob 5, frequency adjustment knob 6, motor start stop switch 7, variable frequency adjustment knob 8, signal generator switch 9 and audio player interface 10; Wherein sound intensity adjusting knob 5 is connected with power amplifier module 21; Frequency adjustment knob 6 is connected with the frequency measurement module, and variable frequency adjustment knob 8 is connected with phonomoter 18.
The signal generating module of apparatus of the present invention electronic control box 1 adopts the ICL8038 chip as function generator, and it can produce waveforms such as single-frequency dextrorotation ripple, square wave, choppy sea, and the acoustic signals power amplifier module amplifies the rear drive speaker operation to it; Handle and drive the result of echo to the LED according to ICL8038 function generator square wave output terminal output signal through the frequency measurement of AT89S52 micro-chip then, be adjusted to the frequency of needs through the regulating frequency knob by 74SL164 and 74SL138; And the acoustical signal of putting speaker according to the end that microphone is experienced; Amplify and handle the back by 37 conversions of MAX1032 analog and AT89S52 micro-chip through UA741 magnifying glass 38 and drive the sound intensity size of echo to the LED by 74SL164 and 74SL138, adjusting sound intensity adjusting knob make the sound intensity reach need intensity of acoustic wave.Can carry out parameters,acoustic during work as required and select and adjust, the frequency setting precision is ± 1 hertz, and sound intensity setting accuracy is at ± 0.1 decibel; The audio player interface that sets on the electronic control box is through an audio conversion switchable various player; The sound wave volume is adjustable in 5～130 decibels of scopes usually; On the variable frequency adjustment knob, be provided with frequency transformer, adopt stepless frequency control technology transferring power, reaction is fast, and rotation speed change is steady, thereby realizes the optimum matching of power train and motor working condition.
This installs the AC power that used power supply is 220V/50Hz, and the casing rear portion is provided with the supply lead of energized, and electronic control box is provided with battery main switch.
When the present invention carries out sonic treatment; Sterilization earlier; Again the microbiological specimens carrier is put into the claw type steel bushing of circular hanging scaffold; Display control module through electronic control box is accurately modulated various processing parameters, lets quantize realization sonic treatment process through synthetic sound signal through power amplifying system driving dynamicloudspeaker.
Working process of the present invention is following: energized, and power turn-on switch 32 is pressed uv lamp switch 3 then, carries out sterilization in the preceding case of sound stimulation; Open sliding door 13, can place sample, if the solid culture sample stimulates for a long time, promptly adopt claw type steel bushing 27 on the circular hanging scaffold 25 to install and given the test agent is fixed down, petridish is uncapped and is inverted preparation and accepts processing; If the short period of time stimulates solid sample, when perhaps the shallow-layer culture sample being handled, then claw type steel bushing 27 is installed up and given the test agent is fixed; After sample places, shut sliding door, the signal generation of closing, power magnification and display control module mains switch 9 are selected required waveform through waveform system selector switch SA1 (being 33 among Fig. 1); The acoustic signals that is produced by the ICL8038 function generator amplifies rear drive overhead speaker 29 (being BLS1 among Fig. 5) through the TDA7294 power amplifier or speaker 28 (being BLS2 among Fig. 5) work is put at the end.Handle and by 74SL164 and the 74SL138 driving echo result to the LED through the frequency measurement of AT89S52 micro-chip according to ICL8038 function generator square wave output terminal output signal then, through regulating frequency adjusting knob 6 (being the RP1 among Fig. 5) with frequency adjustment to the needs frequency; And to put speaker 28 or overhead speaker 29 according to the end that microphone 26 (being the BM among Fig. 7) is experienced (be BLS2 among BLS1 or Fig. 5 among Fig. 5; Also be the BLS among Fig. 7) acoustical signal; Amplify and handle the back by conversion of MAX1032 analog and AT89S52 micro-chip through the UA741 magnifying glass and drive the sound intensity size of echo to the LED by 74SL164 and 74SL138, adjusting sound intensity adjusting knob 5 (RP2 among Fig. 5) make the sound intensity reach need intensity of acoustic wave.Open motor start switch 7 subsequently; Utilize variable frequency adjustment knob 8 to regulate the rotating speed of motor at needs; And place situation per sample, select the end to put speaker 28 or 29 pairs of samples of overhead speaker carry out stimulation process through speaker system selector switch 34 (being the SA2 among Fig. 5).If when the sample of deep-layer liquid cultivation is handled; The regulative mode of its acoustic waveform, frequency and intensity and other operation is with above-mentioned, only is the pallesthesiometer that the overhead speaker changes waterproof into directly to be reached under the liquid level get final product.Also but shutdown signal producer switch 9 connects various types of sound such as player outputting music, noise through audio player interface 10; In addition, according to the experiment needs, circular hanging scaffold 25 can be adjusted the position on adjusting screw mandrel 24, during work, can open fluorescent lamp 31, sees through form 11 and observes the intravital sample stimulus situation of main tank; So be that capable of using device realized the sonic treatment to biological sample.After stimulating end; With variable frequency adjustment knob 8 motor speed is reduced to zero; Sound intensity adjusting knob 5 and frequency adjustment knob 6 transfer to the sound intensity and frequency minimum, turn off motor start stop switch 7 then successively, speaker system selector switch 34; Waveform system selector switch 33, signal generation, power magnification and display control module mains switch 9; Open switch for fluorescent lamp 4, open sliding door 13 and take out sample, turn off total power switch 32 and get final product.
Following examples are used for explaining the concrete method of use of a kind of biomass cells culture apparatus that stimulates based on audible sound of the present invention and the effect that reaches, and the present invention are not had any restriction.
Adopt a kind of microorganism cells culture apparatus that stimulates based on audible sound of the present invention, operate according to the invention described above device working process, it is 100 decibels that intensity is set, and frequency is respectively 1000 hertz, the dextrorotation ripple of 5000 hertz and 10000 hertz; Respectively the intestinal bacteria of cultivating in three groups of LB solid mediums (treatment group) are handled; Stimulation mode is sonic treatment 3h, stops 1h, and then handles 3 hours; Stop 1 hour again;, after incubation time arrives 24 hours, count each treatment group respectively and do not accept the bacterium colony formation situation in the sound stimulation sample (control group), the relatively relative colony forming efficiecy situation of each treatment group with this; The definition of bacterium colony rate is as follows relatively:
The result shows that colibacillary relative colony forming efficiecy bacterium is significantly higher than control group, has arrived 134.5%, 121.2% and 122.8% respectively through the audible sound processing.
Embodiment 2
Adopt a kind of microorganism cells culture apparatus that stimulates based on audible sound of the present invention, operate according to the invention described above device working process, frequency is set is 1000 hertz, intensity is respectively 90,100,110,120 decibels dextrorotation ripple; Respectively the intestinal bacteria of cultivating in three groups of LB solid mediums (treatment group) are handled; Stimulation mode is sonic treatment 3 hours, stops 1 hour, and then handles 3 hours; Stop 1 hour again; With this after incubation time arrives 24 hours, count each treatment group respectively with the intestinal bacteria of not accepting to cultivate in the sound stimulation LB solid medium (control group) in bacterium colony form situation, the relative colony forming efficiecy situation of each treatment group of comparison; The definition of bacterium colony rate is as follows relatively:
The result shows; Frequency is identical; After the audible sound that intensity is different was handled intestinal bacteria, intestinal bacteria had shown visibly different growth conditions, when intensity is 100 decibels; Colony forming efficiecy has reached 141.6% relatively, and colibacillary relative colony forming efficiecy is merely 81.2% when intensity of acoustic wave reaches 110 decibels.
Embodiment 3
Adopt a kind of microorganism cells culture apparatus that stimulates based on audible sound of the present invention, operate according to the invention described above device working process, it is 100 decibels that intensity is set, and frequency is respectively 1000 hertz, the dextrorotation ripple of 5000 hertz and 10000 hertz; Respectively the intestinal bacteria of cultivating in the LB solid medium that contains 3% sodium-chlor (treatment group) are handled; Stimulation mode is sonic treatment 3 hours; Stop 1 hour, and then handled 3 hours, stop 1 hour again; With this after incubation time arrives 24 hours; Respectively counting each handle with the bacterium colony of not accepting in the sound stimulation sample and form situation, and contain the intestinal bacteria cultivated in the LB solid medium of 3% sodium-chlor as control group not accept sound stimulation, relatively the relative colony forming efficiecy situation of each treatment group: the definition of bacterium colony rate is as follows relatively:
The result shows; Audible sound is handled and can obviously be reduced the harm of salt stress to the intestinal bacteria growth under one stable condition, does not accept the sample of sound stimulation under the salt stress, and its colony forming efficiecy is merely 55.21%; And 1000 hertz; The sonic treatment of 5000 hertz and 10000 hertz makes the colibacillary relative colony forming efficiecy under the salt stress reach 65.17%, 75.13% and 80.26% respectively.
Adopt a kind of microorganism cells culture apparatus that stimulates based on audible sound of the present invention; Operate according to the invention described above device working process; Speaker is extend in the intestinal bacteria of LB liquid nutrient medium cultivation, it is 100 decibels that intensity is set, and frequency is respectively 1000 hertz; The dextrorotation ripple of 5000 hertz and 10000 hertz; Respectively the intestinal bacteria (treatment group) in each group liquid nutrient medium are handled, stimulation mode is a continued stimulus, respectively at 7 hours, and 12 hours and 17 hours its OD values of sampling and measuring.The result sees table 1. by showing and can knowing, under the liquid culture condition, audible sound stimulates can obviously promote colibacillary growth, and wherein 5000 hertz sound stimulation is the most remarkable to growth-promoting effect, is 1.84 times of control group relative standardization back OD value.
Under table 1, the liquid culture condition under the different frequency sound stimulation to the influence of the relative OD value of intestinal bacteria
Adopt a kind of microorganism cells culture apparatus that stimulates based on audible sound of the present invention, operate, speaker is extend in the S. cervisiae of liquid nutrient medium cultivation culture temperature 28-30 ℃ according to the invention described above device working process.Using intensity is 100 decibels, 1000 hertz respectively of frequencies, and the dextrorotation ripple of 5000 hertz and 10000 hertz is handled the yeast in the liquid nutrient medium, and stimulation mode is that intermittent type stimulates, every stimulation 3 hours intermittently 3 hours, incubation time is 72 hours.Wait to cultivate the cultivation appearance of finding when finishing through sound stimulation, its alcoholic strength all is significantly increased, and residual sugar content is all below 1%.Especially the sample of 5000 hertz sound stimulation, the residual sugar content in its substratum is merely 0.28%, and alcoholic strength reaches 5.84% (V/V); And the ethanol content in the control group is merely 4.63% (V/V), and residual sugar content is 1.68%.This shows, adopt the audible sound of appropriate frequency and intensity to stimulate the ability that really can significantly improve yeast fermentation production alcohol.
The composition of described LB solid medium: contain 1% Tryptones, 0.5% yeast extract, 1% sodium-chlor by weight percentage in the LB liquid nutrient medium, 1.8% agar, surplus is a water;
The composition of described LB liquid nutrient medium: contain 1% Tryptones, 0.5% yeast extract, 3% sodium-chlor by weight percentage in the LB liquid nutrient medium, surplus is a water;
The described LB solid medium that contains 3% sodium-chlor: contain by weight percentage in the LB liquid nutrient medium of 3% sodium-chlor and contain 1% Tryptones, 0.5% yeast extract, 3% sodium-chlor, 1.8% agar, surplus is a water;
The composition that described GYP liquid nutrient medium is cultivated: contain 12% glucose in the GYP liquid nutrient medium by weight percentage, 0.5% yeast powder, 0.3% peptone, surplus is a water.
Claims (8)
1. microorganism cells culture apparatus that stimulates based on audible sound; It is characterized in that: comprise main box (19), Power Component and electronic control box (1); Described Power Component comprises transmission shaft (14), phonomoter (18), regulates screw mandrel (24) and circular hanging scaffold (25); Transmission shaft (14) vertically is arranged on the central authorities at main box (19) top through drive bearing (15); And be connected with phonomoter (18) through drive pulley (16) and transmission belt (17); The top that transmission shaft (14) passes main box (19) extend in the main box (19), and regulate screw mandrel (24) and be arranged on the end that transmission shaft (14) is positioned at main box (19) inside, and transmission shaft (14) and the coaxial setting of adjusting screw mandrel (24); Through the circular hanging scaffold (25) that has been threaded, main box (19) inside is equipped with uv lamp (30) and fluorescent lamp (31) in the bottom of regulating screw mandrel (24); Each face of main box (19) inwall all is equipped with acoustic panel (23); Main box (19) inner top is equipped with overhead speaker (28); The bottom is equipped with the end and puts speaker (29); In electronic control box (1), be provided with signal generating module (20), power amplifier module (21) and display control module (22); Overhead speaker (28) is put speaker (29) end of with and is connected with signal generating module (20) through power amplifier module (21); Signal generating module (20) is connected with display control module (22) through frequency measurement module (35), in main box (19), also is provided with the microphone (26) that is used for measurement of sound, and microphone (26) is measured module (36) through the sound intensity and is connected with display control module (22).
2. a kind of microorganism cells culture apparatus that stimulates based on audible sound as claimed in claim 1 is characterized in that: operation viewing window (11) is housed on the described main box (19), and main box (19) one sides are equipped with sliding door handle (13).
3. a kind of microorganism cells culture apparatus that stimulates based on audible sound as claimed in claim 1; It is characterized in that: described adjusting screw mandrel (24) is provided with two hex-nuts, and two hex-nuts are clipped in the middle circular hanging scaffold (25) and are fixed on and regulate on the screw mandrel (24).
4. a kind of microorganism cells culture apparatus that stimulates based on audible sound as claimed in claim 1 is characterized in that: on described circular hanging scaffold (25) circumference 6 claw type steel bushings (27) are installed evenly at least.
5. a kind of microorganism cells culture apparatus that stimulates based on audible sound as claimed in claim 4; It is characterized in that: 10mm is wide, 2mm is thick by two for described claw type steel bushing (27); The long spring steel plate of 100mm combines; The spring steel plate right-angled intersection also is fixed on round tray (25) bottom surface with screw, and spring steel plate fixed distance point 20mm place turns up to the direction that deviates from round tray and forms 75 degree angles; The top of spring steel plate bends to the point of fixity direction.
6. a kind of microorganism cells culture apparatus that stimulates based on audible sound as claimed in claim 1, it is characterized in that: described acoustic panel is the cloth art acoustic panel.
7. a kind of microorganism cells culture apparatus that stimulates based on audible sound as claimed in claim 1; It is characterized in that: described electronic control box (1) is provided with display panel (2), uv lamp switch (3), switch for fluorescent lamp (4), sound intensity adjusting knob (5), frequency adjustment knob (6), motor start stop switch (7), variable frequency adjustment knob (8), signal generator switch (9) and audio player interface (10); Wherein sound intensity adjusting knob (5) is connected with power amplifier module (21); Frequency adjustment knob (6) is connected with the frequency measurement module, and variable frequency adjustment knob (8) is connected with phonomoter (18).
8. a kind of microorganism cells culture apparatus that stimulates based on audible sound as claimed in claim 1 is characterized in that: described main box (19) bottom is equipped with castor (12).
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| CN106434623A (en) * | 2016-08-18 | 2017-02-22 | 深圳大学 | Method for controlling growth of escherichia coli by means of surface acoustic wave technology |
| CN107557298A (en) * | 2017-10-06 | 2018-01-09 | 大连医科大学 | Music tampering devic and method in a kind of cell experiment |
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| CN101591614A (en) * | 2009-06-23 | 2009-12-02 | 江苏大学 | A kind of ultrasonic enzymatic membrane reactor |
| CN201933089U (en) * | 2010-05-17 | 2011-08-17 | 江南大学 | Frequency control system of ultrasonic wave biological optimization process |
| CN202465707U (en) * | 2012-01-20 | 2012-10-03 | 河南科技大学 | Microbial cell culture device based on audible sound stimulation |
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| CN106434623A (en) * | 2016-08-18 | 2017-02-22 | 深圳大学 | Method for controlling growth of escherichia coli by means of surface acoustic wave technology |
| CN107557298A (en) * | 2017-10-06 | 2018-01-09 | 大连医科大学 | Music tampering devic and method in a kind of cell experiment |
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