CN106434515A - Method for domesticating bacteria strains applied to biochemical treatment of sulfur-containing waste gas in viscose fiber industry - Google Patents
Method for domesticating bacteria strains applied to biochemical treatment of sulfur-containing waste gas in viscose fiber industry Download PDFInfo
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Abstract
The invention provides a method for domesticating bacteria strains applied to biochemical treatment of sulfur-containing waste gas in a viscose fiber industry. The method is characterized in that a single bacteria strain is replaced by a flora formed by mixing dominant desulfurization bacteria and active sludge dominant bacteria; the mixed flora is gradually domesticated by adjusting a gas feeding condition, so that the mixed flora is gradually adapted to a gas source environment; the desulfurization rate, the acid resistance and the capability of preventing sulfur poisoning of the flora are all greatly improved; and the flora is applied to treatment of the sulfur-containing waste gas in the viscose fiber industry.
Description
Technical field
The invention belongs to microbial technology field, more particularly, to a kind of bacterium of viscose rayon industry sulphur-containing exhaust gas biochemical treatment
Plant acclimation method.
Background technology
Viscose rayon is a principal item of staple fibre, is fabricated by by native cellulose, has smooth nice and cool, thoroughly
Gas, antistatic, the dyeing characteristic such as gorgeous, are the fiber species having very much development potentiality, current China viscose rayon industry is universal
There is sulphur-containing exhaust gas discharge beyond standards, the problem that no practical way is processed.Biochemical method waste gas is to utilize certain
Kind or several antibacterial vital movement digestive gas in pollutant component so as to resolve into the harmless components such as carbon dioxide, water,
So that waste gas is purified, it is low to have an operating cost, the advantages of non-secondary pollution, can be applicable to process for producing viscose rayon
Sulphur-containing exhaust gas.Also the device of some Biochemical method waste gas and the preparation of strain is disclosed, for example in prior art
CN200510042281.8 discloses a kind of technique being prepared bacterial desulfurization agent by actinomycetes LD021, and this technique adopts unwrapping wire
Bacterium LD021 carries out seed culture, then through biofermentation obtain desulfurase fermentation liquid, then fermentation liquid is carried out extraction to prepare micro-
Biological desulphurization agent.But the sulphur-containing exhaust gas concentration of viscose rayon industry is higher, and the murder by poisoning to strain is larger, using the preparation of above-mentioned technique
Bacterial desulfurization agent process sulphur-containing exhaust gas effect on driving birds is not good, desulfurized effect is relatively low, and the specific aim of bacterial desulfurization agent is relatively
By force it is impossible to process hydrogen sulfide and the Carbon bisulfide in waste gas simultaneously, the sulphur-containing exhaust gas being therefore applied to viscose rayon industry are processed
It is unable to reach preferable treatment effect.
Content of the invention
In order to solve above-mentioned technical problem, the present invention provides a kind of strain of viscose rayon industry sulphur-containing exhaust gas biochemical treatment
Acclimation method, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, the described sludge concentration of inoculation is 30-50g/L,
Add dehydrated medium in described sludge water again, in sludge water described in every L, add described dehydrated medium 15-30g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 15-30g, described mud 3-5g every time in sludge water described in every L, so that biofilm thickness is reached
To 0.3-0.5mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 5-15g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 100-150g;
(5) the advantage desulfurization bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 2-5h of step (4) gained
Bacterium solution;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 8-15g, described nutrient 3-6g every time, and culture can complete after 30-40 days
Tamed strain.
Further, described mud is over dry mud.
Further, described mud is viscose rayon production waste and generation in the mixing water treatment procedure of sanitary sewage
Mud.Viscose rayon production waste and the mud of generation in the mixing water treatment procedure of sanitary sewage is selected to live for inoculated and cultured
Property mud dominant bacteria can effectively improve the desulfurization degree of strain compared with the mud selecting other kinds, make strain process sulfur-bearing and give up
The effect of gas is more preferable.
Further, a large amount of activated sludge dominant bacterias are contained in described mud, described activated sludge dominant bacteria includes raw branch
At least one in dynamic glue bacterium, Bacillus cercuses, Flavobacterium, unwrapping wire shape Nocard's bacilluss, middle Escherichia.
Further, described advantage desulfurization bacterium includes grate sulfur thiobacillus, thiobacillus thiooxidant, sulfur oxidation Fructus Citri Limoniae born of the same parents bacterium, oxidation
At least one in ferrous Thiobacillus, thiobacillus denitrificanss.
Preferably, described advantage desulfurization bacterium includes ratio of weight and number is 5:3:2 grate sulfur thiobacillus, thiobacillus thiooxidant and sulfur
Oxidation Fructus Citri Limoniae born of the same parents bacterium.
Activated sludge dominant bacteria and advantage desulfurization bacterium are used in mixed way the sulfur dioxide that can simultaneously process in waste gas and two sulfur
Change hydrogen, effectively improve the desulfurization degree of strain.
Further, methods described also comprises the steps:
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 400-600mg/m3, continue 2-3h;Then
It is passed through the waste gas that sulfur content is 1000-1500mg/m3, continue 4-6h;It is passed through the waste gas that sulfur content is 2200-2800mg/m3 again,
Continue 7-10h and can complete tamed strain.
During strain domestication pass through adjust inlet air conditions, make sulfur content in air inlet be gradually increased to strain is carried out by
Step domestication, so as to gradually adapt to source of the gas environment, can prevent strain from sulfur poisoning situation impact desulfurized effect.
Further, keep dissolved oxygen concentration in bacterium solution to be 3-8mg/L in described aeration process, the anti-of strain can be improved
Sulfur poisoning ability, it is to avoid strain Poisoning Effect desulfurized effect during processing sulphur-containing exhaust gas.
Further, in described water COD value be 120-200mg/L, total values of nitrogen might be 10-20mg/L, total phosphorus value be 2-5mg/
L, pH value are 7.2~7.8.
By strain be dissolved in COD value be 120-200mg/L, total values of nitrogen might be 10-20mg/L, total phosphorus value be 2-5mg/L, pH value
For being conducive in 7.2~7.8 water improving the multiplication capacity of strain, so that strain can be processed after domestication more containing
Sulfur waste gas.
Further, in described step (4), the concentration of oxygen supply is 380-450mg/L.It is 380-450mg/L using oxygen concentration
Gas divulge information in bacterium solution oxygen supply be conducive to improve strain multiplication capacity.
Further, the condition of culture in described step (6) is:Fermentation jar temperature is controlled to be 23-28 DEG C, rotating speed is 100-
150rpm, ventilation are 0.1-0.3vvm.Cultivate strain using above-mentioned condition of culture to be conducive to improving the multiplication capacity of strain.
Further, described dehydrated medium includes each composition of following weight portion:
Glucose 80-120 D- xylose 15-30 carbamide 15-30
Sodium potassium tartrate tetrahydrate 8-12 potassium dihydrogen phosphate 3-8 disodium hydrogen phosphate 3-8.
Using by glucose, D- xylose, carbamide, sodium potassium tartrate tetrahydrate, potassium dihydrogen phosphate and phosphoric acid during strain domestication
The dehydrated medium of disodium hydrogen preparation can improve the anti-sulfur poisoning ability of strain, it is to avoid strain is processing the process of sulphur-containing exhaust gas
Middle Poisoning Effect desulfurized effect.
Further, described dehydrated medium also includes each composition of following weight portion:
Propylgallate 3-5 lauryl alcohol 1-3 sodium salicylate 0.5-1.5.
In dehydrated medium used in strain domestication process, addition propylgallate, lauryl alcohol and sodium salicylate can
To improve the acid resistance of strain, improve strain survival rate in acid condition.
Further, described nutrient includes each composition of following weight portion:
Mannan 10-15 biotin 5-10 glucosan 5-10
Sodium starch glycol 1-3 lactose 1-3.
Prepare using by mannan, biotin, glucosan, sodium starch glycol and lactose during strain domestication
Nutrient can improve the acid resistance of strain, improve strain survival rate in acid condition.
The strain domestication mode that the present invention provides adopts advantage desulfurization bacterium and the flora of activated sludge dominant bacteria mixing to replace
Single strain, is progressively tamed so as to gradually adapt to source of the gas environment by adjusting inlet air conditions to mixed bacterial, flora
Desulfurization degree, acid-resisting and prevent the ability of sulfur poisoning to be both greatly improved it is adaptable to process the sulfur-bearing of viscose rayon industry
Waste gas.
Specific embodiment
Embodiment 1
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud is sewage treatment plant two
Mud in heavy pond, the described sludge concentration of inoculation is 30g/L, then adds dehydrated medium in described sludge water, dirty described in every L
Described dehydrated medium 15g is added in muddy water;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 15g, described mud 3g every time in sludge water described in every L, so that biofilm thickness is reached
0.3mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 5g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 100g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 2h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 8g, described nutrient 3g every time, and culture can complete taming and dociling of strain after 30 days
Change.
Embodiment 2
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud is sewage treatment plant two
Mud in heavy pond, the described sludge concentration of inoculation is 40g/L, then adds dehydrated medium in described sludge water, dirty described in every L
Described dehydrated medium 20g is added in muddy water;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 10g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, and culture can complete taming and dociling of strain after 35 days
Change.
Embodiment 3
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud produces for viscose rayon
Sewage mixes, with sanitary sewage, the mud producing in water treatment procedure, and the described sludge concentration of inoculation is 50g/L, then to described dirt
Add dehydrated medium in muddy water, in sludge water described in every L, add described dehydrated medium 30g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 30g, described mud 5g every time in sludge water described in every L, so that biofilm thickness is reached
0.5mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 15g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 150g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 5h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 15g, described nutrient 6g every time, and culture can complete taming and dociling of strain after 40 days
Change.
Embodiment 4
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud produces for viscose rayon
Sewage mixes, with sanitary sewage, the mud producing in water treatment procedure, and the described sludge concentration of inoculation is 40g/L, then to described dirt
Add dehydrated medium in muddy water, in sludge water described in every L, add described dehydrated medium 20g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 10g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, and culture can complete taming and dociling of strain after 35 days
Change.
Embodiment 5
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud produces for viscose rayon
Sewage mixes, with sanitary sewage, the mud producing in water treatment procedure, and the described sludge concentration of inoculation is 50g/L, then to described dirt
Add dehydrated medium in muddy water, in sludge water described in every L, add described dehydrated medium 30g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 30g, described mud 5g every time in sludge water described in every L, so that biofilm thickness is reached
0.5mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 15g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 150g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 5h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 15g, described nutrient 6g every time, cultivates 40 days;
(7) being passed through sulfur content in the mixed bacteria liquid through culture is 600mg/m3Waste gas, continue 3h;Then pass to contain
Sulfur content is 1500mg/m3Waste gas, continue 6h;Being passed through sulfur content again is 2800mg/m3Waste gas, continue 10h can complete strain
Domestication.
Embodiment 6
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud is sewage treatment plant two
Mud in heavy pond, the described sludge concentration of inoculation is 40g/L, then adds dehydrated medium in described sludge water, dirty described in every L
Described dehydrated medium 20g is added in muddy water;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 10g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, cultivates 35 days;
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 500mg/m3, continue 2.5h;Then pass to
Sulfur content is the waste gas of 1200mg/m3, continues 5h;It is passed through the waste gas that sulfur content is 2500mg/m3 again, continue 8h and can complete bacterium
The domestication planted.
Embodiment 7
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud is sewage treatment plant two
Mud in heavy pond, the described sludge concentration of inoculation is 30g/L, then adds dehydrated medium in described sludge water, dirty described in every L
Described dehydrated medium 15g is added in muddy water;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 15g, described mud 3g every time in sludge water described in every L, so that biofilm thickness is reached
0.3mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 5g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 100g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 2h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 3mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 8g, described nutrient 3g every time, and culture can complete taming and dociling of strain after 30 days
Change.
Embodiment 8
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud is sewage treatment plant two
Mud in heavy pond, the described sludge concentration of inoculation is 40g/L, then adds dehydrated medium in described sludge water, dirty described in every L
Described dehydrated medium 20g is added in muddy water;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 10g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 5mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, cultivates 35 days;
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 500mg/m3, continue 2.5h;Then pass to
Sulfur content is the waste gas of 1200mg/m3, continues 5h;It is passed through the waste gas that sulfur content is 2500mg/m3 again, continue 8h and can complete bacterium
The domestication planted.
Embodiment 9
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is viscose rayon production waste
With the mud producing in the mixing water treatment procedure of sanitary sewage, the described sludge concentration of inoculation is 50g/L, COD value in described water
For 200mg/L, total values of nitrogen might be 20mg/L, total phosphorus value be 5mg/L, pH value be 7.8, then in described sludge water add dry powder culture
Base, adds described dehydrated medium 30g in sludge water described in every L;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 30g, described mud 5g every time in sludge water described in every L, so that biofilm thickness is reached
0.5mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 15g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 150g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 5h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 8mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 15g, described nutrient 6g every time, cultivates 40 days;
(7) being passed through sulfur content in the mixed bacteria liquid through culture is 600mg/m3Waste gas, continue 3h;Then pass to contain
Sulfur content is 1500mg/m3Waste gas, continue 6h;Being passed through sulfur content again is 2800mg/m3Waste gas, continue 10h can complete strain
Domestication.
Embodiment 10
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is secondary sedimentation tank of sewage treatment work
In mud, the described sludge concentration of inoculation be 40g/L, in described water COD value be 150mg/L, total values of nitrogen might be 15mg/L, total phosphorus value
It is 7.5, then adds dehydrated medium in described sludge water for 3mg/L, pH value, in sludge water described in every L, add described dry powder
Culture medium 20g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 10g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, and culture can complete taming and dociling of strain after 35 days
Change.
Embodiment 11
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is viscose rayon production waste
With the mud producing in the mixing water treatment procedure of sanitary sewage, the described sludge concentration of inoculation is 50g/L, COD value in described water
For 200mg/L, total values of nitrogen might be 20mg/L, total phosphorus value be 5mg/L, pH value be 7.8, then in described sludge water add dry powder culture
Base, adds described dehydrated medium 30g in sludge water described in every L;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 30g, described mud 5g every time in sludge water described in every L, so that biofilm thickness is reached
0.5mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 15g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, and the concentration of described oxygen supply is 450mg/L, then
Add described dehydrated medium in described advantage desulfurization bacterium bacterium solution, in advantage desulfurization bacterium bacterium solution described in every L, add described dry powder
Culture medium 150g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 5h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 8mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 15g, described nutrient 6g every time, cultivates 40 days;
(7) being passed through sulfur content in the mixed bacteria liquid through culture is 600mg/m3Waste gas, continue 3h;Then pass to contain
Sulfur content is 1500mg/m3Waste gas, continue 6h;Being passed through sulfur content again is 2800mg/m3Waste gas, continue 10h can complete strain
Domestication.
Embodiment 12
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is secondary sedimentation tank of sewage treatment work
In mud, the described sludge concentration of inoculation be 40g/L, in described water COD value be 150mg/L, total values of nitrogen might be 15mg/L, total phosphorus value
It is 7.5, then adds dehydrated medium in described sludge water for 3mg/L, pH value, in sludge water described in every L, add described dry powder
Culture medium 20g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 10g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, and the concentration of described oxygen supply is 400mg/L, then
Add described dehydrated medium in described advantage desulfurization bacterium bacterium solution, in advantage desulfurization bacterium bacterium solution described in every L, add described dry powder
Culture medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, and culture can complete taming and dociling of strain after 35 days
Change.
Embodiment 13
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is viscose rayon production waste
With the mud producing in the mixing water treatment procedure of sanitary sewage, the described sludge concentration of inoculation is 50g/L, COD value in described water
For 200mg/L, total values of nitrogen might be 20mg/L, total phosphorus value be 5mg/L, pH value be 7.8, then in described sludge water add dry powder culture
Base, adds described dehydrated medium 30g in sludge water described in every L;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 30g, described mud 5g every time in sludge water described in every L, so that biofilm thickness is reached
0.5mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 15g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, and the concentration of described oxygen supply is 450mg/L, then
Add described dehydrated medium in described advantage desulfurization bacterium bacterium solution, in advantage desulfurization bacterium bacterium solution described in every L, add described dry powder
Culture medium 150g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 5h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 8mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, and described condition of culture is:Fermentation jar temperature is controlled to be 28 DEG C, rotating speed is
150rpm, ventilation are 0.3vvm, every 12 hours supplement once described dehydrated medium and nutrients, mixed vaccine described in every L
Liquid supplements described dehydrated medium 15g, described nutrient 6g every time, cultivates 40 days;
(7) being passed through sulfur content in the mixed bacteria liquid through culture is 600mg/m3Waste gas, continue 3h;Then pass to contain
Sulfur content is 1500mg/m3Waste gas, continue 6h;Being passed through sulfur content again is 2800mg/m3Waste gas, continue 10h can complete strain
Domestication.
Embodiment 14
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is secondary sedimentation tank of sewage treatment work
In mud, the described sludge concentration of inoculation be 40g/L, in described water COD value be 150mg/L, total values of nitrogen might be 15mg/L, total phosphorus value
It is 7.5, then adds dehydrated medium in described sludge water for 3mg/L, pH value, in sludge water described in every L, add described dry powder
Culture medium 20g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 10g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, and the concentration of described oxygen supply is 400mg/L, then
Add described dehydrated medium in described advantage desulfurization bacterium bacterium solution, in advantage desulfurization bacterium bacterium solution described in every L, add described dry powder
Culture medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, and described condition of culture is:Fermentation jar temperature is controlled to be 25 DEG C, rotating speed is
120rpm, ventilation are 0.2vvm, every 12 hours supplement once described dehydrated medium and nutrients, mixed vaccine described in every L
Liquid supplements described dehydrated medium 10g, described nutrient 5g every time, and culture can complete tamed strain after 35 days.
Embodiment 15
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud is sewage treatment plant two
Mud in heavy pond, the described sludge concentration of inoculation is 30g/L, then adds dehydrated medium in described sludge water, dirty described in every L
Described dehydrated medium 15g is added, described dehydrated medium includes each composition of following weight in muddy water:
Glucose 80g D- xylose 15g carbamide 15g
Sodium potassium tartrate tetrahydrate 8g potassium dihydrogen phosphate 3g disodium hydrogen phosphate 3g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 15g, described mud 3g every time in sludge water described in every L, so that biofilm thickness is reached
0.3mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 5g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 100g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 2h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 8g, described nutrient 3g every time, and culture can complete taming and dociling of strain after 30 days
Change.
Embodiment 16
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud is sewage treatment plant two
Mud in heavy pond, the described sludge concentration of inoculation is 40g/L, then adds dehydrated medium in described sludge water, dirty described in every L
Described dehydrated medium 20g is added, described dehydrated medium includes each composition of following weight in muddy water:
Glucose 100g D- xylose 20g carbamide 20g
Sodium potassium tartrate tetrahydrate 10g potassium dihydrogen phosphate 5g disodium hydrogen phosphate 5g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 10g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 5mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, cultivates 35 days;
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 500mg/m3, continue 2.5h;Then pass to
Sulfur content is the waste gas of 1200mg/m3, continues 5h;It is passed through the waste gas that sulfur content is 2500mg/m3 again, continue 8h and can complete bacterium
The domestication planted.
Embodiment 17
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud is sewage treatment plant two
Mud in heavy pond, the described sludge concentration of inoculation is 30g/L, then adds dehydrated medium in described sludge water, dirty described in every L
Described dehydrated medium 15g is added, described dehydrated medium includes each composition of following weight in muddy water:
Glucose 80g D- xylose 15g carbamide 15g
Sodium potassium tartrate tetrahydrate 8g potassium dihydrogen phosphate 3g disodium hydrogen phosphate 3g
Propylgallate 3g lauryl alcohol 1g sodium salicylate 0.5g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 15g, described mud 3g every time in sludge water described in every L, so that biofilm thickness is reached
0.3mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 5g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 100g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 2h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 8g, described nutrient 3g every time, and culture can complete taming and dociling of strain after 30 days
Change.
Embodiment 18
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud is sewage treatment plant two
Mud in heavy pond, the described sludge concentration of inoculation is 40g/L, then adds dehydrated medium in described sludge water, dirty described in every L
Described dehydrated medium 20g is added, described dehydrated medium includes each composition of following weight in muddy water:
Glucose 100g D- xylose 20g carbamide 20g
Sodium potassium tartrate tetrahydrate 10g potassium dihydrogen phosphate 5g disodium hydrogen phosphate 5g
Propylgallate 4g lauryl alcohol 2g sodium salicylate 1g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 10g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 5mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, cultivates 35 days;
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 500mg/m3, continue 2.5h;Then pass to
Sulfur content is the waste gas of 1200mg/m3, continues 5h;It is passed through the waste gas that sulfur content is 2500mg/m3 again, continue 8h and can complete bacterium
The domestication planted.
Embodiment 19
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud is sewage treatment plant two
Mud in heavy pond, the described sludge concentration of inoculation is 30g/L, then adds dehydrated medium in described sludge water, dirty described in every L
Described dehydrated medium 15g is added in muddy water;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 15g, described mud 3g every time in sludge water described in every L, so that biofilm thickness is reached
0.3mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 5g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 100g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 2h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 8g, described nutrient 3g every time, and described nutrient includes following weight portion
Each composition:
Mannan 10g biotin 5g glucosan 5g
Sodium starch glycol 1g lactose 1g,
Culture can complete tamed strain after 30 days.
Embodiment 20
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in tap water mud being added 35 DEG C and obtain sludge water, described mud is sewage treatment plant two
Mud in heavy pond, the described sludge concentration of inoculation is 40g/L, then adds dehydrated medium in described sludge water, dirty described in every L
Described dehydrated medium 20g is added, described dehydrated medium includes each composition of following weight in muddy water:
Glucose 100g D- xylose 20g carbamide 20g
Sodium potassium tartrate tetrahydrate 10g potassium dihydrogen phosphate 5g disodium hydrogen phosphate 5g
Propylgallate 4g lauryl alcohol 2g sodium salicylate 1g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described tap water and makes advantage desulfurization bacterium bacterium
Liquid, contains described advantage desulfurization bacterium 10g in advantage desulfurization bacterium bacterium solution described in every L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 5mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, cultivates 35 days;
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 500mg/m3, continue 2.5h;Then pass to
Sulfur content is the waste gas of 1200mg/m3, continues 5h;It is passed through the waste gas that sulfur content is 2500mg/m3 again, under described nutrient includes
Each composition of row weight portion:
Mannan 12g biotin 8g Dextran 8 g
Sodium starch glycol 2g lactose 2g,
Continue 8h and can complete tamed strain.
Reference examples 1
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, advantage desulfurization described in every L
Described advantage desulfurization bacterium 10g is contained in bacterium bacterium solution;
(2) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(3) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (2) gained
Liquid;
(4) the advantage desulfurization bacterium bacterium solution after described activation is poured in fermentation tank and cultivated, every 12 hours supplements one
Secondary described dehydrated medium and nutrient, mixed bacteria liquid described in every L supplements described dehydrated medium 10g, described nutrient every time
5g, culture can complete tamed strain after 35 days.
Reference examples 2
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is secondary sedimentation tank of sewage treatment work
In mud, the described sludge concentration of inoculation be 40g/L, then in described sludge water add dehydrated medium, sludge water described in every L
Described dehydrated medium 20g of middle addition;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 10g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 10mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, cultivates 35 days;
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 500mg/m3, continue 2.5h;Then pass to
Sulfur content is the waste gas of 1200mg/m3, continues 5h;It is passed through the waste gas that sulfur content is 2500mg/m3 again, continue 8h and can complete bacterium
The domestication planted.
Reference examples 3
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is secondary sedimentation tank of sewage treatment work
In mud, the described sludge concentration of inoculation be 40g/L, in described water COD value be 150mg/L, total values of nitrogen might be 15mg/L, total phosphorus value
It is 7.5, then adds dehydrated medium in described sludge water for 3mg/L, pH value, in sludge water described in every L, add described dry powder
Culture medium 20g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 10g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, and the concentration of described oxygen supply is 350mg/L, then
Add described dehydrated medium in described advantage desulfurization bacterium bacterium solution, in advantage desulfurization bacterium bacterium solution described in every L, add described dry powder
Culture medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, and culture can complete taming and dociling of strain after 35 days
Change.
Reference examples 4
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is secondary sedimentation tank of sewage treatment work
In mud, the described sludge concentration of inoculation be 40g/L, in described water COD value be 150mg/L, total values of nitrogen might be 15mg/L, total phosphorus value
It is 7.5, then adds dehydrated medium in described sludge water for 3mg/L, pH value, in sludge water described in every L, add described dry powder
Culture medium 20g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 10g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, and the concentration of described oxygen supply is 400mg/L, then
Add described dehydrated medium in described advantage desulfurization bacterium bacterium solution, in advantage desulfurization bacterium bacterium solution described in every L, add described dry powder
Culture medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, and described condition of culture is:Fermentation jar temperature is controlled to be 30 DEG C, rotating speed is
180rpm, ventilation are 0.05vvm, every 12 hours supplement once described dehydrated medium and nutrients, mixed vaccine described in every L
Liquid supplements described dehydrated medium 10g, described nutrient 5g every time, and culture can complete tamed strain after 35 days.
Reference examples 5
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is secondary sedimentation tank of sewage treatment work
In mud, the described sludge concentration of inoculation be 40g/L, then in described sludge water add dehydrated medium, sludge water described in every L
Described dehydrated medium 20g of middle addition, described dehydrated medium includes each composition of following weight:
Glucose 100g D- xylose 20g carbamide 20g
Potassium dihydrogen phosphate 5g disodium hydrogen phosphate 5g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 10g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 5mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, cultivates 35 days;
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 500mg/m3, continue 2.5h;Then pass to
Sulfur content is the waste gas of 1200mg/m3, continues 5h;It is passed through the waste gas that sulfur content is 2500mg/m3 again, continue 8h and can complete bacterium
The domestication planted.
Reference examples 6
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is secondary sedimentation tank of sewage treatment work
In mud, the described sludge concentration of inoculation be 40g/L, then in described sludge water add dehydrated medium, sludge water described in every L
Described dehydrated medium 20g of middle addition, described dehydrated medium includes each composition of following weight:
Glucose 100g peptone 20g carbamide 20g
Sodium potassium tartrate tetrahydrate 10g potassium dihydrogen phosphate 5g disodium hydrogen phosphate 5g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 10g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 5mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, cultivates 35 days;
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 500mg/m3, continue 2.5h;Then pass to
Sulfur content is the waste gas of 1200mg/m3, continues 5h;It is passed through the waste gas that sulfur content is 2500mg/m3 again, continue 8h and can complete bacterium
The domestication planted.
Reference examples 7
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is secondary sedimentation tank of sewage treatment work
In mud, the described sludge concentration of inoculation be 40g/L, then in described sludge water add dehydrated medium, sludge water described in every L
Described dehydrated medium 20g of middle addition, described dehydrated medium includes each composition of following weight:
Glucose 100g D- xylose 20g carbamide 20g
Sodium potassium tartrate tetrahydrate 10g potassium dihydrogen phosphate 5g disodium hydrogen phosphate 5g
Lauryl alcohol 2g sodium salicylate 1g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 10g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 5mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, cultivates 35 days;
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 500mg/m3, continue 2.5h;Then pass to
Sulfur content is the waste gas of 1200mg/m3, continues 5h;It is passed through the waste gas that sulfur content is 2500mg/m3 again, continue 8h and can complete bacterium
The domestication planted.
Reference examples 8
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is secondary sedimentation tank of sewage treatment work
In mud, the described sludge concentration of inoculation be 40g/L, then in described sludge water add dehydrated medium, sludge water described in every L
Described dehydrated medium 20g of middle addition, described dehydrated medium includes each composition of following weight:
Glucose 100g D- xylose 20g carbamide 20g
Sodium potassium tartrate tetrahydrate 10g potassium dihydrogen phosphate 5g disodium hydrogen phosphate 5g
Propylgallate 4g ascorbic acid 2g sodium salicylate 1g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 10g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 5mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, cultivates 35 days;
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 500mg/m3, continue 2.5h;Then pass to
Sulfur content is the waste gas of 1200mg/m3, continues 5h;It is passed through the waste gas that sulfur content is 2500mg/m3 again, continue 8h and can complete bacterium
The domestication planted.
Reference examples 9
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is secondary sedimentation tank of sewage treatment work
In mud, the described sludge concentration of inoculation be 40g/L, then in described sludge water add dehydrated medium, sludge water described in every L
Described dehydrated medium 20g of middle addition, described dehydrated medium includes each composition of following weight:
Glucose 100g D- xylose 20g carbamide 20g
Sodium potassium tartrate tetrahydrate 10g potassium dihydrogen phosphate 5g disodium hydrogen phosphate 5g
Propylgallate 4g lauryl alcohol 2g sodium salicylate 1g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 10g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 5mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, cultivates 35 days;
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 500mg/m3, continue 2.5h;Then pass to
Sulfur content is the waste gas of 1200mg/m3, continues 5h;It is passed through the waste gas that sulfur content is 2500mg/m3 again, under described nutrient includes
Each composition of row weight portion:
Mannan 12g biotin 8g Dextran 8 g
Lactose 2g,
Continue 8h and can complete tamed strain.
Reference examples 10
A kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment, comprises the steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, described mud is secondary sedimentation tank of sewage treatment work
In mud, the described sludge concentration of inoculation be 40g/L, then in described sludge water add dehydrated medium, sludge water described in every L
Described dehydrated medium 20g of middle addition, described dehydrated medium includes each composition of following weight:
Glucose 100g D- xylose 20g carbamide 20g
Sodium potassium tartrate tetrahydrate 10g potassium dihydrogen phosphate 5g disodium hydrogen phosphate 5g
Propylgallate 4g lauryl alcohol 2g sodium salicylate 1g;
(2) once described dehydrated medium and institute are supplemented from beginning in second day of seed sludge is daily to described sludge water
State mud, supplement described dehydrated medium 20g, described mud 4g every time in sludge water described in every L, so that biofilm thickness is reached
0.4mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, often
Described advantage desulfurization bacterium 10g is contained in advantage desulfurization bacterium bacterium solution described in L;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds in described advantage desulfurization bacterium bacterium solution
Enter described dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 120g;
(5) the advantage desulfurization bacterium bacterium after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 3h of step (4) gained
Liquid, keeps dissolved oxygen concentration in bacterium solution to be 5mg/L in described aeration process;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will
Described mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, every L
Described mixed bacteria liquid supplements described dehydrated medium 10g, described nutrient 5g every time, cultivates 35 days;
(7) it is passed through, in the mixed bacteria liquid through culture, the waste gas that sulfur content is 500mg/m3, continue 2.5h;Then pass to
Sulfur content is the waste gas of 1200mg/m3, continues 5h;It is passed through the waste gas that sulfur content is 2500mg/m3 again, under described nutrient includes
Each composition of row weight portion:
Mannan 12g biotin 8g lauryl alcohol 8g
Sodium starch glycol 2g lactose 2g,
Continue 8h and can complete tamed strain.
The impact analysis to strain desulfurization degree for the strain domestication method
Using embodiment 2, embodiment 4, reference examples 1 strain domestication method domesticated strain, then will be same amount of on
State the strain after domestication to add biological treatment device for exhaust gas to process containing concentration of hydrogen sulfide is 1000mg/m3, Carbon Disulfide
For 1200mg/m3Sulphur-containing exhaust gas, measure strain desulfurization degree, the results are shown in Table 1.
Table 1 strain desulfurization degree is evaluated
Embodiment 2 | Embodiment 4 | Reference examples 1 | |
Desulfurization degree (%) | 83.7 | 99.8 | 69.3 |
From above-mentioned result of the test:Desulfurization degree using the strain of the strain domestication method domestication of embodiment 2 is better than adopting
With the desulfurization degree of the strain of the strain domestication method domestication of reference examples 1, illustrate using advantage desulfurization bacterium and activated sludge dominant bacteria
It is used in mixed way process sulphur-containing exhaust gas compared with individually sulphur-containing exhaust gas being processed using advantage desulfurization bacterium, can process in waste gas simultaneously
Sulfur dioxide and hydrogen sulfide, effectively improve the desulfurization degree of strain.
Desulfurization degree using the strain of the strain domestication method domestication of embodiment 4 is better than the strain domestication using embodiment 2
The desulfurization degree of the strain of method domestication, illustrates selection of activated sludge viscose rayon production waste used and sanitary sewage in strain domestication
The mud producing in mixing water treatment procedure is used for inoculated and cultured activated sludge dominant bacteria than selects other mud can be effective
Improve the desulfurization degree of strain, make the effect of strain process sulphur-containing exhaust gas more preferable.
The impact analysis to strain anti-sulfur poisoning ability for the strain domestication method
It is respectively adopted embodiment 2, embodiment 6, embodiment 8, embodiment 16, reference examples 2, reference examples 5, the bacterium of reference examples 6
Kind of acclimation method domesticated strain, and when the strain after domestication being all applied to one section of the sulphur-containing exhaust gas of process for producing viscose rayon
Between, judge the anti-sulfur poisoning ability of strain by measuring the strain survival rate after processing sulphur-containing exhaust gas, the results are shown in Table 2.
Table 2 strain anti-sulfur poisoning merit rating
From above-mentioned result of the test:The strain of the strain domestication method domestication being provided using embodiment 6 is processed sulfur-bearing and gives up
Survival rate after gas is higher than the strain of the strain domestication method domestication being provided using embodiment 2, therefore passes through to adjust inlet air conditions,
Make sulfur content in air inlet be gradually increased to strain is progressively tamed so as to gradually adapt to source of the gas environment, strain can be prevented
Sulfur poisoning situation impact desulfurized effect occurs.
Survival rate after the strain of the strain domestication method domestication being provided using embodiment 8 processes sulphur-containing exhaust gas is higher than to adopt
The strain of the strain domestication method domestication of embodiment 6 and reference examples 2 offer, keeps dissolved oxygen concentration in bacterium solution in aeration process
The anti-sulfur poisoning ability of strain can be improved for 3-8mg/L, it is to avoid strain Poisoning Effect during processing sulphur-containing exhaust gas takes off
Sulfur effect.
Survival rate after the strain of the strain domestication method domestication being provided using embodiment 16 processes sulphur-containing exhaust gas is higher than to adopt
The strain of the strain domestication method domestication being provided with embodiment 8, reference examples 5 and reference examples 6, therefore adopts during strain domestication
Permissible with the dehydrated medium prepared by glucose, D- xylose, carbamide, sodium potassium tartrate tetrahydrate, potassium dihydrogen phosphate and disodium hydrogen phosphate
Improve the anti-sulfur poisoning ability of strain, it is to avoid strain Poisoning Effect desulfurized effect during processing sulphur-containing exhaust gas.
The impact analysis to strain multiplication capacity for the strain domestication method
Using embodiment 2, embodiment 10, embodiment 12, embodiment 14, reference examples 3, reference examples 4 strain domestication method
The strain of domestication equal number, and count the strain sum obtaining after cultivating 35 days, calculate strain proliferation times, result is shown in
Table 3.
Table 3 strain multiplication capacity is evaluated
Strain proliferation times | |
Embodiment 2 | 3.4×102 |
Embodiment 10 | 8.7×103 |
Embodiment 12 | 1.2×105 |
Embodiment 14 | 2.4×106 |
Reference examples 3 | 9.1×103 |
Reference examples 4 | 2.0×105 |
The above results show, the proliferation times using the strain of the strain domestication method domestication of embodiment 10 are more than using real
Apply example 2 strain domestication method domestication strain, illustrate by strain be dissolved in COD value be 120-200mg/L, total values of nitrogen might be 10-
20mg/L, total phosphorus value are to be conducive to improving the multiplication capacity of strain in the water that 2-5mg/L, pH value are 7.2~7.8, so that bacterium
Plant and can process more sulphur-containing exhaust gas through domestication.
Proliferation times using the strain of the strain domestication method domestication of embodiment 12 are more than using embodiment 10 and comparison
The strain of the strain domestication method domestication of example 3, illustrate that the gas for 380-450mg/L is divulged information oxygen supply in bacterium solution using oxygen concentration
Be conducive to improving the multiplication capacity of strain.
Proliferation times using the strain of the strain domestication method domestication of embodiment 14 are more than using embodiment 12 and comparison
The strain of the strain domestication method domestication of example 4, illustrates to be 23-28 DEG C in fermentation jar temperature, rotating speed is 100-150rpm, ventilation
For carrying out to strain under the condition of culture of 0.1-0.3vvm cultivating the multiplication capacity being conducive to improving strain.
The acid proof impact analysis to strain of strain domestication method
It is respectively adopted the strain domestication method domesticated strain of embodiment 16, embodiment 18, embodiment 20, reference examples 7-10,
And by domestication after strain be inoculated in fluid medium with 2% inoculum concentration, under the conditions of 37 DEG C cultivate, culture 24h after
Take 10ml bacterium solution to be centrifuged under aseptic condition, abandon supernatant, be centrifuged again after adding physiological saline solution washing, abandon supernatant, add
The hydrochloric acid of 5ml 0.1mol/L, separately sampled dilution after putting into 37 DEG C of thermostat water bath constant temperature 2h after concussion uniformly applies flat board, enters
Row count plate, measures the survival rate of bacterium, the results are shown in Table 4.
Table 4 strain acid resistance is evaluated
The strain of the strain domestication method domestication being provided using embodiment 18 survival rate in acid condition is higher than to adopt
The strain of the strain domestication method domestication that embodiment 16, reference examples 7 and reference examples 8 provide, therefore makes during strain domestication
Add propylgallate, lauryl alcohol and sodium salicylate can improve the acid resistance of strain in dehydrated medium, improve bacterium
Plant survival rate in acid condition.
The strain of the strain domestication method domestication being provided using embodiment 20 survival rate in acid condition is higher than to adopt
The strain of the strain domestication method domestication that embodiment 18, reference examples 9 and reference examples 10 provide, therefore adopts during strain domestication
With the acidproof of strain can be improved by nutrient prepared by mannan, biotin, glucosan, sodium starch glycol and lactose
Property, improve strain survival rate in acid condition.
Finally it should be noted that above example is only in order to illustrate technical scheme and unrestricted, although reference
Preferred embodiment has been described in detail to the present invention, it will be understood by those within the art that, can be to the present invention's
Technical scheme is modified or equivalent, and without deviating from the spirit and scope of technical solution of the present invention, it all should be covered
In the middle of scope of the presently claimed invention.
Claims (10)
1. a kind of strain domestication method of viscose rayon industry sulphur-containing exhaust gas biochemical treatment is it is characterised in that methods described includes
Following steps:
(1) carry out inoculation in water mud being added 35 DEG C and obtain sludge water, the described sludge concentration of inoculation is 30-50g/L, then to
Add dehydrated medium in described sludge water, in sludge water described in every L, add described dehydrated medium 15-30g;
(2) once described dehydrated medium and described dirt are supplemented from beginning in second day of seed sludge is daily to described sludge water
Mud, supplements described dehydrated medium 15-30g, described mud 3-5g every time in sludge water described in every L, so that biofilm thickness is reached
0.3-0.5mm;
(3) mud biofilm after 3 days, advantage desulfurization bacterium is dissolved in 35 DEG C of described water and makes advantage desulfurization bacterium bacterium solution, every L institute
State and in advantage desulfurization bacterium bacterium solution, contain described advantage desulfurization bacterium 5-15g;
(4) divulge information in the advantage desulfurization bacterium bacterium solution making oxygen supply 30min, then adds institute in described advantage desulfurization bacterium bacterium solution
State dehydrated medium, in advantage desulfurization bacterium bacterium solution described in every L, add described dehydrated medium 100-150g;
(5) the advantage desulfurization bacterium bacterium solution after activation will be obtained after the advantage desulfurization bacterium bacterium solution continuous aeration 2-5h of step (4) gained;
(6) the advantage desulfurization bacterium bacterium solution after activation and the sludge water mixing after mud biofilm 3 days are obtained mixed bacteria liquid, will be described
Mixed bacteria liquid is poured in fermentation tank and is cultivated, every 12 hours supplement once described dehydrated medium and nutrients, described in every L
Mixed bacteria liquid supplements described dehydrated medium 8-15g, described nutrient 3-6g every time, and culture can complete strain after 30-40 days
Domestication.
2. according to claim 1 strain domestication method it is characterised in that described mud be viscose rayon production waste and life
The mud producing in the mixing water treatment procedure of sewage alive.
3. according to claim 1 strain domestication method it is characterised in that methods described also comprises the steps:
(7) being passed through sulfur content in the mixed bacteria liquid through culture is 400-600mg/m3Waste gas, continue 2-3h;Then pass to
Sulfur content is 1000-1500mg/m3Waste gas, continue 4-6h;Being passed through sulfur content again is 2200-2800mg/m3Waste gas, continue
7-10h can complete tamed strain.
4. according to claim 1 strain domestication method it is characterised in that in described aeration process keep bacterium solution in dissolved oxygen
Concentration is 3-8mg/L.
5. according to claim 1 strain domestication method it is characterised in that in described water COD value be 120-200mg/L, total
Values of nitrogen might is 10-20mg/L, total phosphorus value is 2-5mg/L, pH value is 7.2~7.8.
6. according to claim 1 strain domestication method it is characterised in that in described step (4) oxygen supply concentration be 380-
450mg/L.
7. according to claim 1 strain domestication method it is characterised in that the condition of culture in described step (6) is:Control
Fermentation jar temperature is 23-28 DEG C, and rotating speed is 100-150rpm, ventilation is 0.1-0.3vvm.
8. according to claim 1 strain domestication method it is characterised in that described dehydrated medium includes following weight portion
Each composition:
Glucose 80-120 D- xylose 15-30 carbamide 15-30
Sodium potassium tartrate tetrahydrate 8-12 potassium dihydrogen phosphate 3-8 disodium hydrogen phosphate 3-8.
9. according to claim 8 strain domestication method it is characterised in that described dehydrated medium also includes following weight portion
Each composition:
Propylgallate 3-5 lauryl alcohol 1-3 sodium salicylate 0.5-1.5.
10. according to claim 1 strain domestication method it is characterised in that described nutrient includes each of following weight portion
Composition:
Mannan 10-15 biotin 5-10 glucosan 5-10
Sodium starch glycol 1-3 lactose 1-3.
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