CN106432522A - Method for leaching polysaccharides and phenethyl alcohol glycoside - Google Patents
Method for leaching polysaccharides and phenethyl alcohol glycoside Download PDFInfo
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- CN106432522A CN106432522A CN201610859459.6A CN201610859459A CN106432522A CN 106432522 A CN106432522 A CN 106432522A CN 201610859459 A CN201610859459 A CN 201610859459A CN 106432522 A CN106432522 A CN 106432522A
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/18—Acyclic radicals, substituted by carbocyclic rings
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Abstract
The invention discloses a method for leaching polysaccharides and phenethyl alcohol glycoside from raw materials such as herba cistanches. The method is characterized by comprising placing the raw materials with the herba cistanches in cold water at the temperature of 0-30 DEG C and pretreating the raw materials; leaching the polysaccharides and the phenethyl alcohol glycoside by the aid of hot water at the temperature of 80-100 DEG C. The method has the advantages of simplicity, easiness in operation, high extraction and separation speeds, zero volatile organic solvents, little environmental pollution and high polysaccharide and phenethyl alcohol glycoside selectivity.
Description
Technical field
The application is related to a kind of method of leaching polysaccharide and benzyl carbinol glycosides from the raw material containing saline cistanche, belongs to natural product
The separation and Extraction field of thing active ingredient.
Background technology
Cistanche deserticola Orobanchaceae, calls big rue, very little rue, desert cistanche, looks into dry announcement Asia (Mongolian).Saline cistanche is that one kind colonizes in sand
The parasitic plant of unconcerned trees sacsaoul root, has high medical value.Benzyl carbinol glycoside containing kidney-replenishing, taste in saline cistanche
Ring dilute ether terpenoid of kidney yin, can both tonifying kidney-yins, and kidney-replenishing, negative and positive are put down and are mended, and mend and not high, warm and not dry, will not
Get angry.Prove according to modern medicine study, Cistanche deserticola in a kind of energy excited hypophysis, adrenal cortex or has similar adrenal cortex
Hormone-like effect, adjusts the rare medicinal herbs of body's immunity, and polysaccharide content in its extract is higher, the immunity of polysaccharide component
Humidification plays main impact in the strong of saline cistanche and help effectiveness.
The at present extraction of biological substance and separate through frequently with two aqueous phase extraction method, although the method has condition temperature
With, the advantage easily amplifying and can operate continuously, but the aqueous two-phase due to being constituted using organic solvent miscible with water and salt
Extraction system, haves the shortcomings that solvent is volatile, environmental pollution is big and selectivity is low.
Content of the invention
Method or a kind of polysaccharide and the benzene of a kind of leaching polysaccharide and benzyl carbinol glycosides according to the one side of the application, are provided
The preparation method of ethanol glycosides, with saline cistanche etc. as raw material, is first pre-processed to raw material with cold water, reusable heat water extract polysaccharide and
Benzyl carbinol glycosides.The method have simple, easy to operate, extract that separating rate is fast, do not need volatile organic solvent, environmental pollution little
Advantage.
Described leaching polysaccharide and benzyl carbinol glycosides method it is characterised in that first by the raw material containing saline cistanche be placed in 0 DEG C～
After pre-processing in 30 DEG C of cold water, then leached with 80～100 DEG C of hot water.
Preferably, in described cold water, pretreatment and hot water leaching process are carried out under ultrasonic time and/or microwave.Described cold water
Middle pretreatment and hot water leaching process, are carried out in using the container equipped with microwave device and ultrasonic probe.Locate in advance in cold water
In reason and hot water leaching process, one of them can be opened simultaneously, simultaneously close off or only be opened to microwave device and ultrasonic probe.
As one preferred embodiment, methods described is first the raw material containing saline cistanche to be placed in 0 DEG C～30 DEG C
With after ultrasonic pretreatment in cold water, then leached with 80～100 DEG C of hot water.
As one preferred embodiment, methods described is first the raw material containing saline cistanche to be placed in 0 DEG C～30 DEG C
After pre-processing in cold water, then with being leached under ultrasonic wave in 80～100 DEG C of hot water.
As a further preferred embodiment, methods described be first by the raw material containing saline cistanche be placed in 0 DEG C～
With after ultrasonic pretreatment in 30 DEG C of cold water, with being leached under ultrasonic wave in 80～100 DEG C of hot water.
As one preferred embodiment, methods described is first the raw material containing saline cistanche to be placed in 0 DEG C～30 DEG C
With after Microwave Pretreatment in cold water, then leached with 80～100 DEG C of hot water.
As one preferred embodiment, methods described is first the raw material containing saline cistanche to be placed in 0 DEG C～30 DEG C
After pre-processing in cold water, then with being leached under microwave in 80～100 DEG C of hot water.
As one preferred embodiment, methods described is first the raw material containing saline cistanche to be placed in 0 DEG C～30 DEG C
With after Microwave Pretreatment in cold water, with being leached under microwave in 80～100 DEG C of hot water.
As one preferred embodiment, methods described is with surpassing in the cold water be first placed in cynomorium songaricum piece 0 DEG C～30 DEG C
After sound preconditioning, with being leached under microwave and under ultrasonic in 80～100 DEG C of hot water.
Preferably, described ultrasonic frequency range is 20kHz～50kHz.It is further preferred that described ultrasonic frequency model
Enclose for 22kHz～30kHz.
Preferably, described ultrasonic power density is 5W/L～100W/L.It is further preferred that described ultrasonic power density is
20W/L～40W/L.
Preferably, described microwave frequency range is 2GHz～3GHz.It is further preferred that described microwave frequency range is
2.4GHz～2.5GHz.
Preferably, described Microwave Power Density is 100W/L～500W/L.It is further preferred that described Microwave Power Density
For 200W/L～300W/L.
In the application, described power density processes the ultrasonic or micro- of the upper applying of material (liquid-solid phase gross weight) for unit weight
The power of ripple, unit is W/kg.
Preferably, the fruit of Chinese wolfberry and/or date are contained in described raw material；
In raw material, saline cistanche, matrimony vine, the mass ratio of date are：
Saline cistanche：Matrimony vine：Date=40～100:10～70:60～120.
Preferably, the temperature of cold water raw material being pre-processed is 0～25 DEG C.It is further preferred that carrying out to raw material
The temperature of the cold water of pretreatment is 0～10 DEG C.
Preferably, the temperature of hot water raw material being leached is 80～95 DEG C.It is further preferred that soaking to raw material
The temperature of the hot water taking is 80～90 DEG C.
As a kind of embodiment, the method for described leaching polysaccharide and benzyl carbinol glycosides is it is characterised in that at least include following
Step：
A () raw material is placed in 0 DEG C～30 DEG C of water, directly soak or be no less than 30min in ultrasonic middle immersion, obtain
System I；
B () heating stepses (a) gained system I is to 80 DEG C～100 DEG C, and keep system temperature under ultrasonic and/or microwave
80 DEG C～100 DEG C are no less than 30min, obtain system II；
C the solid phase in () separation system II and liquid phase, liquid phase is designated as extract I, and solid phase is designated as solid I；Extract I is put
In 0～10 DEG C be no less than 24h after, through filter residue, concentrated in vacuo after, obtain final product polysaccharide and the crude product of benzyl carbinol glycosides extract.
In above-mentioned embodiment it is preferable that in step (a) raw material and water mass ratio=1:5～9.Further preferably
Ground, mass ratio=1 of raw material and water in step (a):6～8.
As a kind of embodiment, the method for described leaching polysaccharide and benzyl carbinol glycosides, at least comprise the following steps：
A () raw material is placed in 0 DEG C～30 DEG C of water, directly soak or be no less than 30min in ultrasonic middle immersion, obtain
System I；
B () heating stepses (a) gained system I is to 80 DEG C～100 DEG C, and keep system temperature under ultrasonic and/or microwave
80 DEG C～100 DEG C are no less than 30min, obtain system II；
C the solid phase in () separation system II and liquid phase, liquid phase is designated as extract I, and solid phase is designated as solid I；
D () solid I is placed in 80～100 DEG C of water, and keep under ultrasonic and/or microwave 80 DEG C of system temperature～
100 DEG C are no less than 20min, obtain system III；
E the solid phase in () separation system III and liquid phase, liquid phase is designated as extract II, and solid phase is designated as solid II；By extract
II mix with the extract I of step (c) gained be placed in 2～8 DEG C be no less than 24h after, through filter residue, concentrated in vacuo after, that is, much
Sugar and the crude product of benzyl carbinol glycoside compound.
As a kind of embodiment, the method for described leaching polysaccharide and benzyl carbinol glycosides, at least comprise the following steps：
A () raw material is placed in 0 DEG C～30 DEG C of water, directly soak or be no less than 30min in ultrasonic middle immersion, obtain
System I；
B () heating stepses (a) gained system I is to 80 DEG C～100 DEG C, and keep system temperature under ultrasonic and/or microwave
80 DEG C～100 DEG C are no less than 30min, obtain system II；
C the solid phase in () separation system II and liquid phase, liquid phase is designated as extract I, and solid phase is designated as solid I；
D () solid I is placed in 80～100 DEG C of water, and keep under ultrasonic and/or microwave 80 DEG C of system temperature～
100 DEG C are no less than 20min, obtain system III；
E the solid phase in () separation system III and liquid phase, liquid phase is designated as extract II, and solid phase is designated as solid II；
F () solid II is placed in 80～100 DEG C of water, and keep under ultrasonic and/or microwave 80 DEG C of system temperature～
100 DEG C are no less than 10min, obtain system IV；
G the solid phase in () separation system IV and liquid phase, liquid phase is designated as extract III, and solid phase is designated as solid III；To extract
Liquid III mix with extract I, step (e) the gained extract II of step (c) gained be placed in 2～8 DEG C be no less than 24h after, warp
Filter residue, concentrated in vacuo after concentrated in vacuo after, obtain final product the crude product of polysaccharide and benzyl carbinol glycosides.
In above-mentioned embodiment it is preferable that in step (a) raw material and water mass ratio=1:5～7.Further preferably
Ground, mass ratio=1 of raw material and water in step (a):6.
Preferably, in step (d) solid I and water mass ratio=1:4～6.It is further preferred that it is solid in step (d)
Body I and mass ratio=1 of water:5.
Preferably, in step (f) solid II and water mass ratio=1:3～5.It is further preferred that it is solid in step (f)
Body II and mass ratio=1 of water:4.
Preferably, in step (b), step (d) and step (f), the temperature of water is 80～95 DEG C.Step (b), step (d) and
In step (f), the temperature of water is 80～90 DEG C.
Preferably, in step (a), soak time is 30min～2h.As a kind of specific embodiment, in step (a)
Soak time be 1 hour.
Preferably, the retention time in step (b) is 30min～2h.As a kind of specific embodiment, step (b)
In retention time be 60min.
Preferably, the retention time in step (d) is 20min～1h.As a kind of specific embodiment, step (b)
In retention time be 40min.
Preferably, the retention time in step (f) is 10min～1h.As a kind of specific embodiment, step (b)
In retention time be 30min.
As a kind of embodiment, the method for polysaccharide and benzyl carbinol glycosides in the raw material such as described leaching saline cistanche, its feature exists
In, using multistage cross flow leaching, at least comprise the following steps：
A () raw material is placed in 0 DEG C～30 DEG C of water, soak and be no less than 30min, obtain system I；
B () heating stepses (a) gained system I is at 80 DEG C～100 DEG C, and carry out multistage cross flow under ultrasonic and/or microwave
Leaching, every grade of leaching time is no less than 30min；
(c) collect and blend step (b) in liquid phases at different levels after multilevel leaching, be placed in 0～10 DEG C be no less than 24h after,
Through filter residue, concentrated in vacuo after, obtain final product the crude product of polysaccharide and benzyl carbinol glycosides.
Preferably, described multistage cross flow leaches and leaches for three-level cross-flow, wherein：
Mass ratio=1 of raw material and water in first order leaching:5～9；
Mass ratio=1 of raw material and water in the leaching of the second level:4～7；
Mass ratio=1 of raw material and water in third level leaching:4～7.
It is further preferred that described multistage cross flow leaches leaching for three-level cross-flow, wherein：
Mass ratio=1 of raw material and water in first order leaching:6～8；
Mass ratio=1 of raw material and water in the leaching of the second level:5～6；
Mass ratio=1 of raw material and water in third level leaching:5～6.
As a kind of embodiment, the method for polysaccharide and benzyl carbinol glycosides in the raw material such as described leaching saline cistanche, its feature exists
In using subsequent leaching, at least comprising the following steps：
A () raw material is placed in 0 DEG C～30 DEG C of water, directly soak or be no less than 30min in ultrasonic middle immersion, obtain
System I；
(b) heating stepses (a) gained system I at 80 DEG C～100 DEG C, and under the ultrasonic and/or microwave in continuously soaked
Take, the charging mass ratio of raw material and water is 1:3～7；
C liquid phase extract that () step (b) obtains be placed in 0～10 DEG C be no less than 24h after, through filter residue, concentrated in vacuo after, that is,
Obtain the crude product of polysaccharide and benzyl carbinol glycosides.
Preferably, in subsequent leaching, the charging mass ratio of solid phase and liquid phase is 1:4～8.
Preferably, described subsequent leaching leaches for continuous flow upstream, carries out in counter-flow moving bed leacher.
It is further preferred that described subsequent leaching leaches for continuous flow upstream, carry out in Kennedy's formula leacher.
Preferably, the saline cistanche in raw material is the sheet of thickness 2～8mm.It is further preferred that the saline cistanche in raw material is
The sheet of thickness 3～6mm.
Preferably, the water content of the saline cistanche in raw material is 1～15wt%.It is further preferred that the saline cistanche in raw material
Water content be 5～10wt%.
Preferably, in step (a) raw material and water mass ratio=1:5～8；
Preferably, in step (a) raw material and water mass ratio=1:6.
Preferably, described it is placed in that to concentrate in vacuum concentration pot be bar in 70～90 DEG C of temperature, pressure 0.05～0.07MPa
Carry out concentrated in vacuo under part.
As a kind of specific embodiment, comprise the following steps that：Weigh raw material：Saline cistanche 40-100 weight portion, matrimony vine
Sub 10～70 weight portions, date 60～120 weight portion, raw material cleaning is extracted, for the first time after ultrasonic middle immersion 2～4h
Plus cold water (weight is 6 times of raw material), heat after boiling and keep micro-boiling to extract in microwave 60 minutes, extract is filtered to take
Go out；Add water (weight is 5 times of raw material) for the second time, and heating is boiled rear and keeps micro-boiling to extract 40 minutes, by extract in microwave
Filter and take out；Third time adds water (weight is 5 times of raw material), and heating is boiled rear and keeps micro-boiling to extract in microwave 40 minutes, will
Extract filters and takes out.Merge No. three extracts, place it in the environment that temperature is 0～10 DEG C and stand more than 24 hours,
Extract supernatant, cross 100 mesh sieves and carry out being filtered to remove precipitation, filtrate is added in vacuum concentration pot, is 70～90 DEG C in temperature,
Vacuum is to carry out reduced pressure concentration under 0.05～0.07MPa, is 1.30～1.40 (60～65 DEG C) to relative density, in thick
When stop heating, carry out sterile filling.
According to the another aspect of the application, provide a kind of composition containing polysaccharide and benzyl carbinol glycosides, said composition is soluble in
Hot water, is not required to separately plus the material such as colloid, carbohydrate, and high fat of blood, diabetes patient are also direct-edible, be capable of enhance immunity,
Improve the purpose of fatigue state.
The described composition containing polysaccharide and benzyl carbinol glycosides, is the polysaccharide and benzene being prepared according to above-mentioned any one method
The crude product of ethanol glycosides；The crude product of described polysaccharide and benzyl carbinol glycosides is 1.3～1.4 with the relative density of water at 60～65 DEG C.
The beneficial effect of the application includes but is not limited to：
Leaching polysaccharide provided herein and the method for benzyl carbinol glycosides, have simple, easy to operate, extraction separating rate
Hurry up, do not need little, selectively high to the polysaccharide and benzyl carbinol glycosides advantage of volatile organic solvent, environmental pollution.According to the application
Polysaccharide and the composition of benzyl carbinol glycosides that methods described prepares, are soluble in hot water, are not required to separately add the material such as colloid, carbohydrate,
High fat of blood, diabetes patient are also direct-edible, are capable of enhance immunity, improve the purpose of fatigue state.
Specific embodiment
With reference to embodiment in detail the application is described in detail, but the application is not limited to these embodiments.
In embodiment, the raw material saline cistanche of employing is Alashan Desert Region saline cistanche, and the fruit of Chinese wolfberry is peaceful Xia Zhongning matrimony vine.
In embodiment, total starches content in polysaccharide and benzyl carbinol glycosides crude product, measured using phenol-sulfuric acid colometry.
In embodiment, benzyl carbinol glycosides content in polysaccharide and benzyl carbinol glycosides crude product, adopt《Chinese Pharmacopoeia》One middle meat desert
Rong's content assaying method high effective liquid chromatography for measuring.
In embodiment, the microbiological indicator in sample adopts GB4789《National food safety standard》Measure
Embodiment 1
Weigh the raw material containing saline cistanche, the fruit of Chinese wolfberry and date and add cold water, (be specifically shown in Table 1) under ultrasonic wave or microwave and soak
After bubble a period of time, heating systems to a certain temperature simultaneously (are specifically shown in Table 1) holding a period of time (first under ultrasonic wave or microwave
Secondary hot water extracts), it is centrifugally separating to obtain solid I and extract I；Add a certain amount of water in solid I, be heated to a certain temperature
And keep a period of time (second hot water extracts), it is centrifugally separating to obtain solid II and extract II；One is added in solid II
Quantitative water, is heated to a certain temperature and keeps a period of time (extraction of third time hot water), be centrifugally separating to obtain solid III and carry
Take liquid III.Merge extract I, extract II and extract III, place it in and in the environment that temperature is 0～10 DEG C, stand 24
Hour, cross 100 mesh screen and remove precipitation, filtrate is added in vacuum concentration pot, under a certain temperature and pressure, by all filters
Liquid is concentrated into the thick crude product poured out, obtain described polysaccharide and benzyl carbinol glycosides.
The sample number into spectrum of gained polysaccharide and benzyl carbinol glycosides crude product is shown in Table 1 with the condition of concrete ultrasonic wave, microwave treatment,
Wherein, second hot water of sample number into spectrum G2 extracts and extracts identical microwave treatment conditions using with first time hot water, and sample is compiled
Second of number G3 and third time hot water extract using with first time hot water extraction identical microwave treatment conditions.
Table 1
| Sample number into spectrum | Pretreatment mode | Hot water treatment mode |
| G1 | Ultrasonic wave (20kHz, 100W/kg) | Microwave (2.4GHz, 300W/kg) |
| G2 | Ultrasonic wave (25kHz, 40W/kg) | Microwave (2.5GHz, 200W/kg) |
| G3 | Ultrasonic wave (30kHz, 30W/kg) | Microwave (2.45GHz, 250W/kg) |
The sample number into spectrum of the crude product of gained polysaccharide and benzyl carbinol glycosides refers to table 2 with the relation of concrete reaction condition.
Table 2
Embodiment 2
Raw material proportioning according to G1 in embodiment 1 weighs saline cistanche, the fruit of Chinese wolfberry and date, plus cold water in 10 DEG C in ultrasonic wave
After soaking 1h in (22kHz, 40W/kg), it is heated to 85 DEG C of beginnings and carries out continuous flow upstream leaching in Kennedy's formula leacher, Gu
The charging mass ratio of phase and liquid phase is 1:5, gained sample is designated as G2.
Embodiment 3
Insoluble matter in sample G1～G3 and total plate count are detected, the parameter of products obtained therefrom is as shown in table 3, G1's
Paste-forming rate is 46%, and total solid is 70.3wt%.
Table 3
Benzyl carbinol glycosides in sample G1～G3 and total starches content are determined, result is as shown in table 4.
Table 4
| Sample number into spectrum | Polyoses content | Benzyl carbinol glycosides content |
| G1 | 40.80% | 21.6mg/ml |
| G2 | 37.23% | 25.4mg/ml |
| G3 | 35.60% | 23.8mg/ml |
Be can be seen that by table 4 data has good selectivity according to herein described method to polysaccharide and benzyl carbinol glycosides.
The above, be only several embodiments of the application, and not the application is done with any type of restriction although this Shen
Please with preferred embodiment disclose as above, but and be not used to limit the application, any those skilled in the art, do not taking off
In the range of technical scheme, make a little variation using the technology contents of the disclosure above or modification is all equal to
Effect case study on implementation, belongs in the range of technical scheme.
Claims (10)
1. a kind of method of leaching polysaccharide and benzyl carbinol glycosides is it is characterised in that be first placed in 0 DEG C～30 by the raw material containing saline cistanche
DEG C cold water in pre-process after, then leached with 80～100 DEG C of hot water.
2. method according to claim 1 is it is characterised in that pretreatment and hot water leaching process are ultrasonic in described cold water
Carry out down and/or under microwave.
3. the method described in claim 1 is it is characterised in that described ultrasonic frequency range is 20kHz～50kHz；
It is further preferred that described ultrasonic frequency range is 22kHz～30kHz；
Preferably, described ultrasonic power density is 5W/kg～100W/kg；
It is further preferred that described ultrasonic power density is 20W/kg～40W/kg；
Preferably, described microwave frequency range is 2GHz～3GHz；
It is further preferred that described microwave frequency range is 2.4GHz～2.5GHz；
Preferably, described Microwave Power Density is 100W/kg～500W/kg；
It is further preferred that described Microwave Power Density is 200W/kg～300W/kg.
4. method according to claim 1 is it is characterised in that contain the fruit of Chinese wolfberry and/or date in described raw material；
In raw material, saline cistanche, matrimony vine, the mass ratio of date are：
Saline cistanche：Matrimony vine：Date=40～100:10～70:60～120.
5. method according to claim 1 is it is characterised in that at least comprise the following steps：
A () raw material is placed in 0 DEG C～30 DEG C of water, directly soak or be no less than 30min in ultrasonic middle immersion, obtain system
I；
B () heating stepses (a) gained system I is to 80 DEG C～100 DEG C, and keep 80 DEG C of system temperature under ultrasonic and/or microwave
～100 DEG C are no less than 30min, obtain system II；
C the solid phase in () separation system II and liquid phase, liquid phase is designated as extract I, and solid phase is designated as solid I；Extract I is placed in 0
～10 DEG C be no less than 24h after, through filter residue, concentrated in vacuo after, obtain final product the crude product of polysaccharide and benzyl carbinol glycosides.
6. method according to claim 1 is it is characterised in that using multistage cross flow leaching, at least comprise the following steps：
A () raw material is placed in 0 DEG C～30 DEG C of water, directly soak or be no less than 30min in ultrasonic middle immersion, obtain system
I；
B () heating stepses (a) gained system I is at 80 DEG C～100 DEG C, and carry out multistage cross flow leaching under ultrasonic and/or microwave,
Every grade of leaching time is no less than 30min；
C the liquid phases at different levels after multilevel leaching in () collection blend step (b), are placed in 0～10 DEG C no less than after 24h, through filter
Slag, concentrated in vacuo after, obtain final product polysaccharide and benzyl carbinol glycosides crude product.
7. method according to claim 1, it is characterised in that adopting subsequent leaching, at least comprises the following steps：
A () raw material is placed in 0 DEG C～30 DEG C of water, directly soak or be no less than 30min in ultrasonic middle immersion, obtain system
I；
(b) heating stepses (a) gained system I at 80 DEG C～100 DEG C, and under the ultrasonic and/or microwave in carry out subsequent leaching, former
The charging mass ratio of material and water is 1:3～7；
C liquid phase extract that () step (b) obtains be placed in 0～10 DEG C be no less than 24h after, through filter residue, concentrated in vacuo after, that is, much
Sugar and benzyl carbinol glycosides crude product.
8. method according to claim 5 it is characterised in that in step (a) raw material and water mass ratio=1:5～9；
Preferably, in step (a) raw material and water mass ratio=1:6～8.
9. method according to claim 6 it is characterised in that described multistage cross flow leach for three-level cross-flow leaching, wherein,
Mass ratio=1 of raw material and water in first order leaching:5～9；
Mass ratio=1 of raw material and water in the leaching of the second level:4～7；
Mass ratio=1 of raw material and water in third level leaching:4～7；
Preferably,
Mass ratio=1 of raw material and water in first order leaching:6～8；
Mass ratio=1 of raw material and water in the leaching of the second level:5～6；
Mass ratio=1 of raw material and water in third level leaching:5～6.
10. a kind of composition containing polysaccharide and benzyl carbinol glycosides is it is characterised in that be according to claim 1 to 9 any one
Polysaccharide and benzyl carbinol glycosides crude product that method prepares；Described polysaccharide is relative with water at 60～65 DEG C with benzyl carbinol glycosides crude product
Density is 1.3～1.4.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610859459.6A CN106432522A (en) | 2016-09-28 | 2016-09-28 | Method for leaching polysaccharides and phenethyl alcohol glycoside |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610859459.6A CN106432522A (en) | 2016-09-28 | 2016-09-28 | Method for leaching polysaccharides and phenethyl alcohol glycoside |
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| CN106432522A true CN106432522A (en) | 2017-02-22 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108524535A (en) * | 2018-04-16 | 2018-09-14 | 天津中医药大学 | The new application of wheat-based diet and pharmaceutical composition containing wheat-based diet |
| CN109463759A (en) * | 2018-12-20 | 2019-03-15 | 周学义 | A kind of extraction of Herba Cistanches effective component and the production method of product |
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