CN1064309A - Concentrated cleaning compositions with high active cellulase - Google Patents
Concentrated cleaning compositions with high active cellulase Download PDFInfo
- Publication number
- CN1064309A CN1064309A CN 92100955 CN92100955A CN1064309A CN 1064309 A CN1064309 A CN 1064309A CN 92100955 CN92100955 CN 92100955 CN 92100955 A CN92100955 A CN 92100955A CN 1064309 A CN1064309 A CN 1064309A
- Authority
- CN
- China
- Prior art keywords
- detergent composition
- cellulase
- endo
- dextranase
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Detergent Compositions (AREA)
Abstract
The present invention relates to the granular detergent composition of cellulase, said composition is " concentrating " form, promptly compares the inorganic filling salt that they have higher density and contain low amount with common detergent composition.In the present composition, this cellulase defines by C14CMC method described in the invention, and preferably includes specific single component endo-dextranase.
Description
The present invention relates to contain the granular detergent composition of cellulase, this detergent composition exists with conc forms.Be that they are compared with common detergent composition and have higher density and contain more a spot of inorganic filling salt.In detergent composition of the present invention, said cellulase comprises the high active cellulase by the C14CMC method definition of this paper narration.The endo-dextranase of preferably a kind of specific one-component of this cellulase.
In the art, be starved of really and not only have good cleaning performance, and have the detergent composition of performance and other fabric nursing advantages of good softening fabrics.
Clean and reduction roughness reagent for the textiles that is used for fabric, the enzyme of loosen collagen fibre element is that the effect of cellulase is familiar with considerable time; GB-A-2,075,028, GB-A-2,095,275 and GB-A-2,094,826 discloses the detergent composition with the cellulase that is used to improve cleaning performance: GB-A-1, and 368,599 disclose the application of the cellulase that is used to reduce the cotton-containing fabrics roughness; U.S.4,435,307 have narrated the application as the detergent additives that reduces roughness of the cellulolytic enzyme that is known as ACXI by Humicola insolens and cut deutero-thereof.
EP-A-0269168 discloses the best detergent composition that contains cellulase, and this detergent composition is prepared in weakly alkaline pH value scope, and comprehensive fabric cleaning, fabric sofetening and fabric nursing performance is provided.
Disclose the cellulase preparation that is used to reduce the cotton-containing fabrics roughness in WO89109259, this cellulase preparation contains the endo-dextranase component that has high endonuclease activity and avidity for Mierocrystalline cellulose.
Yet the practical application of cellulase is subjected to the obstruction of such fact, and promptly cellulase preparation is complicated mixture as those disclosed cellulase preparation in above-mentioned prior art document, and wherein only some is effective aspect fabric nursing.Therefore, be difficult to realize be used for the feasible industrial production of cost of the cellulase of detergent industry, and need to use the plain zymin of this a large amount of fibrids, so that reach the required effect that acts on fabric.
With regard to the applicability of washing composition, the improvement aspect cellulase production does not usually also become to be enough to recognize.Can stipulate to use relevant cellulase choice criteria by disclosed C14CMC method in EP-A-350098 with the washing composition of cellulase.Have now found that the minimum value of removing 10% the radiolabeled carboxymethyl cellulose of immobilization can provide highly active cellulase.In the common unsettled 5 days Mays nineteen ninety applying date of Danish Patent Application No.:1159/90() in the plain enzyme of a preferred fibrid that belongs to the high reactivity definition of the present invention is disclosed, wherein disclosed the cellulase preparation of being made up of similar endo-dextranase component substantially, this similar endo-dextranase component has immunoreactivity for the single vegetative propagation antibody that causes anti-partially purified 43KD cellulase (being derived by Humicola insolens DM1800).
For advantageously this specific endo-dextranase cellulose components of the processing of cellulose material, this discovery makes now possible feasible ground of cost production of cellulose enzyme (for example by using recombinant DNA technology), and allow only to use a spot of cellulase preparation, and reach the required effect that acts on fabric.
On the other hand, selling detergent composition of new generation on the market, preferably be referred to as " concentrated cleaning ", although they have been presented the extensive stock title, for example " Ultra ", " Supra ", " Micro " ...The characteristic of this class detergent composition is, compares with common detergent composition, and they have higher density, and by using " concentrating " detergent composition than the amount of common detergent composition much less, they can reach identical effect.With regard to its composition, just can reflect this characteristic best by more a spot of inorganic filling salt.This class " concentrates " usefulness of detergent composition preferably by saving the prewashing circulation and using dispersion and dispersion device to reach, and above-mentioned dispersion and dispersion device are directly put into the washing machine rotating cylinder when the master washes the circulation beginning.
The detergent composition that the purpose of this invention is to provide conc forms, this detergent composition have higher density and contain small amounts of inorganic fills salt, and it demonstrates best cellulase usefulness.
In EP-A-381397, disclosed the performance of low ionic strength, particularly to the effect of the performance of lipase to enzyme.
Yet, now being surprised to find, concentrated matrix is higher than far away by the desired effect of prior art cellulase (as disclosed in EP-A-381397) the effect of the enzyme that the present invention selects.
Another object of the present invention provides the method for handling fabric in washing machine, and this method comprises that detergent composition of the present invention is used for the master with lower concentration washes circulation.
The present invention relates to contain tensio-active agent, washing assistant and enzyme, if need also contain the granular detergent composition of additive commonly used, it is characterized in that said enzyme comprises cellulase preparation, according to the C14CMC method, this cellulase preparation provides removes at least 10% the radiolabeled carboxymethyl cellulose of immobilization, and said preparation is with 25 * 10
-6%(weight) cellulase protein matter is present in the laundry testing liquid.
Preferably, said cellulase compound is made up of similar endo-dextranase component substantially, this similar endo-dextranase component is for causing that anti-partially purified about ≈ 43KD cellulase is (by Humicola insolens, DSM1800 derives) haplotype breeding antibody have immunoreactivity, or the homologue of said ≈ 43KD endo-dextranase.
This detergent composition is a particle form, it is characterized in that its density is higher than the density of common detergent composition.The density range of the present composition is every liter of composition 550 to 950g when measuring down for 20 ℃, and preferred every liter of composition 650 is to 850g.
With regard to composition, just can represent " concentrating " form of the present composition best by the amount of inorganic filling salt.The composition commonly used of the detergent composition that inorganic filling salt is powder type.In common detergent composition, this amount of filling the salt existence is quite big, is generally the 17-35% of composition total weight.
In the present composition, the amount that said filling salt exists is no more than 15% of composition total weight, preferably is no more than 10%, is most preferably not exceeding 5%.
Inorganic filling salt (as indication in the present composition) is selected from the vitriol and the muriate of basic metal and alkaline-earth metal.
Preferred filling salt is sodium sulfate.
Tensio-active agent
In detergent composition of the present invention, can use various tensio-active agents.United States Patent (USP) 3,664,961(issued to Norris on May 23rd, 1972) in generally enumerated negatively charged ion, nonionic, amphoteric and zwitterionic classes, and provided various tensio-active agents.
The mixture of anion surfactant is particularly suitable for the present invention, the mixture of sulfonate and sulfate surfactant particularly, and its weight ratio is 5: 1 to 1: 2, is preferably 3: 1 to 2: 3, more preferably 3: 1 to 1: 1.Preferred sulfonate comprises having 9 to 15 in the alkyl, particularly has the alkylbenzene sulfonate of 11 to 13 carbon atoms, and α-sulfonated fatty acid methyl ester, and wherein lipid acid is by C
12-C
18Fat source is particularly by C
16-C
18The fat source deutero-.Positively charged ion in each example is a basic metal, preferably sodium.Preferred sulfate surfactant is that the alkyl-sulphate that has 12 to 18 carbon atoms in the alkyl at random mixes with ethoxy sulfate, this ethoxy sulfate has 10 to 20 carbon atoms in its alkyl, preferably have 10 to 16 carbon atoms, and average degree of ethoxylation is 1 to 6.The example of the preferred alkyl-sulphate of the present invention is tallow alkyl sulfate, coconut alkyl-sulphate and C
14-15Alkyl-sulphate.Positively charged ion in each example also is alkali metal cation, preferably sodium.
Being used for a class nonionogenic tenside of the present invention is the condenses of oxyethane and hydrophobic part, and it provides, and to have average hydrophile-lyophile balance (HLB) degree scope be 8 to 17, preferred 9.5 to 13.5, more preferably 10 to 12.5 tensio-active agent.Said hydrophobic (oleophylic) part with regard to its character can be aliphatic series or aromatics, and the length of the polyoxyethylene groups of it and the condensation of any specific hydrophobic base can easily be regulated, so that be created in the water-soluble cpds that has the required balance degree between hydrophilic and the hydrophobic part.
Particularly preferred this class nonionogenic tenside is the C that every mol of alcohol contains the 3-8 moles of ethylene oxide
9-C
15Primary alcohol ethoxylate, particularly every mol of alcohol contains the C of 6-8 moles of ethylene oxide
14-C
15Primary alconol and every mol of alcohol contain the C of 3-5 moles of ethylene oxide
12-C
14Primary alconol.
Another kind of ionic surfactant pack is drawn together the alkyl polyglucoside compound of following general formula
Wherein, z is the part that is obtained by glucose, and R is the saturated hydrophobic alkyl that contains 12 to 18 carbon atoms; T be 0 to 10 and n be 2 or 3; X is 1.3 to 4, and this compound contains less than 10% unreacted Fatty Alcohol(C12-C14 and C12-C18) with less than 50% short-chain alkyl polyglucoside.This compounds and its are disclosed among the EP-B0070077,0075996 and 0094118 in detergent application.
The polyhydroxy fatty acid amide surfactant of following formula also is fit to as nonionogenic tenside
Wherein, R
1Be H, C
1-4Alkyl, 2-hydroxyethyl, 2-hydroxypropyl, or its mixture, R
2Be C
5-31Alkyl and Z are the polyhydroxy alkyls that has the straight-chain alkyl of directly linking at least 3 hydroxyls on its chain, or its alkoxy derivative.Preferred R
1Be methyl, R
2It is straight chain C
11-15Alkyl or alkenyl, as coconut alkyl or its mixture, and Z is obtained as glucose, fructose, maltose, lactose reduction by sugar in reductive amination process.
Another kind of tensio-active agent is semi-polarity tensio-active agent, for example amine oxide.The amine oxide that is fit to is selected from single C
8-C
20, preferred C
10-C
14N-alkyl or alkenyl amine oxide and titanium dioxide propylidene-1, the 3-diamines wherein keeps the N position to be replaced by methyl, hydroxyethyl or hydroxypropyl.
Also having a class tensio-active agent is amphoterics, for example polyamines base class tensio-active agent.
Cats product also can use in detergent composition of the present invention, and the quaternary ammonium surfactant that is fit to is selected from single C
8-C
16, preferred C
10-C
14N-alkyl or alkenyl ammonium surfactant wherein keep the N position to be replaced by methyl, hydroxyethyl or hydroxypropyl.
The mixture of preferred various surfactants, more preferably negatively charged ion-nonionic mixture.And negatively charged ion-nonionic-cation mixt.Particularly preferred mixture is described in English Patent 2040987 and openly apply in 0087914 in Europe.Detergent composition can contain 1-70%(weight) tensio-active agent, but the amount that is present in the tensio-active agent in the present composition usually is 1% to 30%, more preferably 10-25%(weight).
Washing assistant
Builder material generally is to exist with 10 to 60% of detergent composition of the present invention.Composition of the present invention do not contain or do not contain substantially phosphatic washing assistant (the present invention is defined do not contain substantially be the composition amount be less than total detergent builders system 1%), and builder system of the present invention is by water soluble detergency promoter, the water-insoluble washing assistant, or its mixture is formed.
The water-insoluble washing assistant can be a mineral ion exchange material, generally is inorganic hydrated aluminosilicate material, and the synthetic zeolite of hydration more preferably is as hydrated zeolite A, X, B or HS.
Preferred silico-aluminate ion exchange material has the structure cell unit of following general formula
Wherein M is the positively charged ion of calcium exchange, and z and y are at least 6, and the mol ratio of z and y is 1.0 to 0.5, and X is at least 5, is preferably 7.5 to 276, more preferably 10 to 264.Said silico-aluminate material exists with hydrated form, and preferably contains 10 to 28% xln that more preferably contain 18 to 22% water.
Above-mentioned silico-aluminate ion exchange material is characterized in that its particle size diameter is 0.1 to 10 micron, is preferably 0.2 to 4 micron.The mean particle diameter of the ion exchange material that term herein " particle size diameter " representative is given, it is the analytical technology by routine, for example utilizes the micro-mensuration of scanning electronic microscope to measure.Another of said silico-aluminate ion exchange material is characterised in that its calcium ion exchange capacity is at least 200mg equivalent CaCO
3The water hardness/g silico-aluminate (calculating) with anhydrous benchmark, and common scope is 300mg equivalent/g to 352mg equivalent/g.Another of the silico-aluminate ion exchange material of this paper is characterised in that its calcium ion exchange rate, describes in detail and sees GB-1,429,143.
The silico-aluminate ion exchange material that uses in the invention process is commercially available, and can be naturally occurring material, but preferably synthetic deutero-material.The method that is used to produce the silico-aluminate ion exchange material is described in detail in United States Patent (USP) 3,985, in 669.Being used for preferred synthetic crystallization silico-aluminate ion exchange material of the present invention is commercially available material that is called zeolite A, zeolite B, X zeolite, zeolite HS and composition thereof.In particularly preferred embodiments, said crystalline silico-aluminate ion exchange material is zeolite A, and has following general formula
Wherein X is 20 to 30, preferred 27.Has general formula
Na
86((AlO
2)
86(SiO
2)
106) 10.276H
2The X zeolite of O and have general formula Na
6((AlO
2)
6(SiO
2)
6) 7.5H
2The zeolite HS of O also is fit to.
The another kind of water-insoluble inorganic builders material that is fit to is a layered silicate, for example SKS-6(Hoechst).SKS-6 is a kind of by water glass (Na
2Si
2O
5) the layered silicate crystal formed.High Ca
++/ Mg
++Binding capacity mainly is a cationic exchange mechanism.In hot water, this material becomes more solvable.
Water soluble detergency promoter can be monomer or oligopolymer carboxy acid salt chelator.
The carboxylate salt that is fit to that contains a carboxyl comprises the derivative (being disclosed in belgian patent 831,368,821,369 and 821,370) of lactic acid, oxyacetic acid and its ether.The multi-carboxylate of containing two carboxyls comprises that the water-soluble salt of succsinic acid, propanedioic acid, (ethylenedioxy) diacetic acid, toxilic acid, bi oxyacetate, tartrate, tartronic acid and fumaric acid and ether carboxylate (are described in German prospectus 2,446,686 and 2,446,687 and United States Patent (USP) 3,935,257 in), and thionyl carboxylate salt (being described in the belgian patent 840,623).The multi-carboxylate of containing three carboxyls comprises, especially the derivative of water-soluble citrate, aconitate and citraconate and succinate, (be described in English Patent 1 as carboxy methoxy-succinic acid salt, in 379,241), newborn oxygen base succinate (be described in Holland application 7205873 in) and contain oxygen multi-carboxylate material, as 2-oxa--1,1,3-tricarballylic acid salt (be described in English Patent 1,387,447 in).
The multi-carboxylate of containing four carboxyls comprises and contains oxygen disuccinate (be disclosed in English Patent 1,261,829 in), 1,1,2,2-ethane tetracarboxylic acid hydrochlorate, 1,1,3,3-propane tetracarboxylic acid salt and 1,1,2,3-propane tetracarboxylic acid salt.Contain the substituent multi-carboxylate of sulfo group comprise the sulfo-succinic acid salt derivative (be disclosed in English Patent 1,398,421 and 1,398,422 and United States Patent (USP) 3,936,448 in) and sulfonation pyrolysis citric acid (be described in English Patent 1,082,179 in).Be disclosed in English Patent 1,439 and contain the substituent multi-carboxylate of phosphino-, in 000.
Alicyclic ring and heterocycle multi-carboxylate comprise pentamethylene-suitable, suitable, suitable-tetracarboxylic acid hydrochlorate, cyclopentadiene metallide pentacarboxylic acid salt, 2,3,4,5-tetrahydrofuran (THF)-suitable, suitable, suitable-tetracarboxylic acid hydrochlorate, 2,5-tetrahydrofuran (THF)-suitable-dicarboxylate, 2,2,5,5-tetrahydrofuran (THF)-tetracarboxylic acid hydrochlorate, 1,2,3,4,5,6-hexane hexacarboxylic acid salt and polyvalent alcohol, for example carboxymethyl derivant of Sorbitol Powder, mannitol and Xylitol.Aromatic multi-carboxy acid's salt comprises mellitic acid, 1,2,4, the derivative of 5-pyromellitic acid and phthalic acid (be disclosed in English Patent 1,425,343 in).
In above-mentioned multi-carboxylate, preferred multi-carboxylate is the hydroxycarboxylate that each molecule contains three carboxyls of as many as, particularly Citrate trianion.
The preferred builder system that is used for the present composition comprises the mixture of water-insoluble silico-aluminate washing assistant such as zeolite A and water-soluble carboxylate sequestrant such as citric acid.
For the present invention, other builder materials that can form builder system part comprise for example for example organic phosphonate, amino polyalkylene phosphonate and aminopolycanboxylic acid's salt of alkaline carbonate, supercarbonate, silicate and organic substance of inorganic substance.
Other water-soluble organic salts that are fit to are homopolymerization or co-polymeric acids or its salt, and wherein, poly carboxylic acid contains separated at least two carboxyls by no more than two carbon atoms.
This base polymer is disclosed in GB-A-1, in 596,756.The example of this class salt be molecular weight be 2000-5000 the multi-carboxylate and with the multipolymer of maleic anhydride, this molecular weight of copolymer is 20,000 to 70,000, particularly about 40,000.
Cellulase
Defined for the active of the enzyme of various application and the particularly activity of cellulase by different analytical procedures.These methods all attempt to be provided at desired actual evaluation in use properties aspect or relevant with use properties at least mensuration.As being specified among European patent application EP-A-350098.Many methods particularly in these methods of often using of production of cellulose enzyme and the laundry detergent composition use properties of cellulase do not have confidential relation.This is that for above-mentioned working conditions, these active measuring methods are modified because various other working conditions causes.
Be modified in the method described in the EP-A-350098, make the mutual relationship that becomes and have predictability for the evaluation of the cellulase activity in the laundry detergent composition.
Therefore, in order to differentiate the cellulase that is used for cellulase of the present invention and does not constitute the object of the invention, the present invention uses disclosed method in EP-A-350098.The screening method (this method adopt be by the disclosed method of EP-A-350098) that hereinafter is called as the C14CMC method can be described below:
Principle:
The principle of the C14CMC method that is used to screen is under the plain enzyme concn of specific fibre, in washing soln, to measure the immobilization carboxymethyl cellulose of removing (CMC) from the fabric substrate.Use the C14 radiocarbon C14, measure the CMC that removes by the radio-labeling of some CMC.Before cellulose treatment and afterwards, the amount of the radioactivity C14 of simple count on the fabric substrate just can be estimated the activity of cellulase.
The preparation of sample:
The preparation of CMC: prepare radioactivity CMC liquid storage according to the table I.Can obtain radioactivity CMC by the described method of EP-A-350098.
The fabric substrate: the fabric substrate is the thin cotton goods cloth specimen with 5cm * 5cm size.The heart is with the inoculation of the radiolabeled CMC liquid storage of 0.35ml therein, and is then should thin cotton goods soiled cotton air-dry.
CMC's is fixing: for immobilization of radioactive mark CMC on thin cotton goods cloth specimen, use the bottle formula detergency test instrument of being made by German Original Haunau " Linitest Original Haunau ".The metal bottle of this bottle formula detergency test instrument 400ml hard water (4 mmole Ca
++/ liter) be full of, each bottle can use the maximum number of 13 cloth specimens.Then, this bottle is in bottle formula detergency test instrument, and insulation is 40 minutes from 20 to 60 ℃ heating cycle.After the insulation, the rinsing 1 minute in the mobile tap water of said cloth specimen.With their the extruding and make it air-dry at least 30 minutes.
According to the method for EP-A-350098, the cloth sample with immobilized radioactivity CMC also can be used as " blank sample " and measures when not washing.
The processing of sample:
The laundry testing liquid: the composition according to the table II prepares the laundry testing liquid.With its balance to PH be 7.5.This laundry testing liquid is the basic thing that adds the cellulase test sample to it.Before the amount of measuring the cellulase that will add, the care should be used to operation reaches 100% balance and does not dilute this laundry testing liquid by adding water.The amount of the cellulase that is used for this shaker test that adds should make that cellulase protein reaches 25 * 10 in the laundry testing liquid
-6%(weight) (under 14.5 ℃, equal 0.25mg/l).
Washing methods: in the laundry analogy method, handle the soiled cotton of so inoculating with radio-labeling CMC.This clothes washing method is a mimic in the bottle formula detergency test instrument of being made by German Original Haunau " Linitest Original Haunau ".The monolithic soiled cotton is put into 20cm
3In the glass vial.This phial is full of with 10ml laundry testing liquid, then with its sealing, makes it the no leakage body.Can put into the bottle of each bottle formula detergency test instrument with reaching 5 phials.This bottle water is full of, and water is as laundry mimic heat transmission medium.The laundry simulation was carried out 40 minutes in 20 to 60 ℃ heating cycle.
After handling sample, this phial is immersed in the cold water, then every block of soiled cotton is taken out from its phial, and usefulness mobile soft water rinsing in beaker is with its extruding and make it air-dry at least 30 minutes.
Measure:
In order to measure the radio-labeling CMC that removes, use scintillometer, for example LKB1210 ° of Ultrabeta scintillometer.In order to obtain the most accurate result, should follow the manual control instruction of the optimum operation that is used for this specific scintillometer.For example, for LKB 1210 Ultrabeta scintillometer, should carry out according to the following step.The soiled cotton that will measure is put into the plastics phial that is full of with 12ml scintillator liquid (for example deriving from the scintillator 299 of Packard).Made soiled cotton then stable at least 30 minutes.Then, phial is put into LKB 1210Ultrabeta scintillometer, and obtain the corresponding radiocounting of soiled cotton.
For only measure the amount of the CMC that removes owing to cellulase, must measure inoculated simultaneously but in the laundry testing liquid that does not have cellulase to exist processed soiled cotton.So, can be expressed as the activity of cellulase the percentage ratio of the radio-labeling CMC that removes.This percentage ratio calculates by following formula:
The radioactivity CMC%=that removes (XO-XC)/(XO) * 100
Wherein: XO is the radioactivity scintillation counting with the soiled cotton of the laundry testing liquid processing that does not have cellulase to exist
XC is the radioactivity scintillation counting with the soiled cotton of the laundry testing liquid processing that contains cellulase to be evaluated
The statistics foundation, the determining of method:
For correct statistics is provided, should use the statistical study of standard.For given example, owing to use LKB 1210 Ultrabeta scintillometer, have now found that for each radioactivity scintillation counting, can use 3 soiled cotton sample values.For the different methods by inside is checked the gained result mutually, recommend the mensuration and the calculating of use EP-A-350098 " blank sample ".This can find and eliminate mistake.
Interpretation of result:
Described shaker test provide really a kind of fast, unique and method reliably, be used for differentiating cellulase that satisfies the active standard of the present invention and the cellulase that is not the present invention's part.
Have now found that, according to above-mentioned C14CMC method, remove 10% or more fixed radio-labeling CMC show that corresponding cellulase has satisfied needs of the present invention.
Those skilled in the art it is evident that to the present technique art, and the percentage ratio of removing is higher than 10% and shows that corresponding cellulase has higher activity.Therefore, can expect, according to the C14CMC method, under the protein concn of laundry in the testing liquid, can provide remove be higher than 25% or the cellulase that preferably is higher than 50% radio-labeling CMC will reach the better properties index that this cellulase is used for detergent for washing clothes.
What anticipated equally is that the higher concentration cellulase that is used for the C14CMC method will provide the higher percentage ratio of removing.Yet, do not have verified linear relationship between the percentage ratio at cellulase concentration and removing of obtaining thus.
Anticipate in addition,, use the cellulase of higher concentration that the higher percentage ratio of removing will be provided for the C14CMC method.
Table I: radioactivity C14 mark CMC liquid storage (all percentage ratios are all in total solution weight)
| Total CMC ＊(CMC should be the washing composition level CMC with substitution value of about 0.47 to about 0.7) | 99.2×10 -3% |
| Ethanol | 14985.12×10 -3% |
| Deionized water | 84915.68×10 -3% |
| Amount to: | 100% |
*Total CMC contains on-radiation and radioactivity CMC, and radioactivity CMC provides radioactivity, and this radioactivity is enough to make on employed scintillometer clearly reading.For example, radioactivity CMC can have the active of 0.7 millicurie/g and can be mixed.
Table II: laundry testing liquid (all percentage ratios are all in total solution weight)
| Straight chain C 12Alkyl benzene sulphonate (ABS) | 0.110％ |
| Coconut alkyl-sulphate (TEA) salt | 0.040％ |
| C 12-15Alcohol ethoxylate (E07) | 0.100％ |
| Coconut fatty acid | 0.100％ |
| Oleic acid | 0.050％ |
| Citric acid | 0.010％ |
| Trolamine | 0.040％ |
| Ethanol | 0.060％ |
| Propylene glycol | 0.015％ |
| Sodium hydroxide | 0.030％ |
| Sodium formiate | 0.010％ |
| Proteolytic enzyme | 0.006％ |
| Water (2.5 mmoles/rise Ca ＋＋), PH conditioning agent (HCl or NaOH solution) and cellulase | Balance to 100% |
According to the present invention, preferred cellulase is at those cellulases described in the Danish Patent Application 1159/90.For example, the cellulase preparation that is used for the present composition can be made up of similar endo-dextranase component substantially, this similar endo-dextranase component is for causing that anti-highly purified 43KD cellulase is (by Humicola insolens, DSM1800 derives) antibody have immunoreactivity, or the homologue of said 43KD endo-dextranase.
Should be emphasized that the plain enzyme of all fibres of the present invention must satisfy the standard of above-mentioned shaker test.Yet, in Danish Patent Application 1159/90, stipulated other standards, it is combined just with shaker test of the present invention can determine preferred cellulase.
Be preferred for the cellulase preparation in the present composition, except satisfying shaker test, the CMC endonuclease activity that the endo-dextranase component shows is every milligram of total protein concentration at least about 50, preferably at least about 60, especially preferably at least about 90CMC restriction endonuclease unit.The CMC endonuclease activity that especially preferred endo-dextranase component shows is every milligram of total protein concentration 100CMC restriction endonuclease unit at least.
In this article, term " CMC endonuclease activity " is meant that with the endo-dextranase component can be the endoglucanase activity that the ability of glucose, cellobiose and trisaccharide is represented with cellulose degradation, this active mensuration is after being incubated with cellulase preparation of the present invention, undertaken by the reduction of measuring carboxymethyl cellulose (CMC) soltion viscosity, details are as follows.
Can measure CMC restriction endonuclease (endo-dextranase) activity by the reduction of CMC viscosity, be described below: at pH value is 9 o'clock, and preparation contains CMC(Hercules 7 LFD of 35g/l in the 0.1M tris buffer) substrate solution.Enzyme sample to be analyzed is dissolved in the identical damping fluid.The 10ml substrate solution mixes with the 0.5ml enzyme solution, and with its transfer to 40 ℃ of following homothermic viscometers (as Haake VT 181, the NV transmitter, 181rpm) in.Get the viscosity reading after mixing and after 30 minutes as early as possible.The amount that reduces the viscosity to 1/2 enzyme under these conditions is defined as 1 CMC of unit endonuclease activity.
With the method known to the skilled that is skilled in technique in the art, use sds polyacrylamide gel electrophoresis (SDS-PAGE) and measure the molecular weight and the iso-electric point (PI) of the endo-dextranase component that is used for cellulase preparation of the present invention with the isoelectrofocusing of labelled protein respectively.The molecular weight of the specific endo-dextranase component of Ce Dinging is 43KD in this way, and the iso-electric point of this kind endo-dextranase is about 5.1.
The activity of cellobiose hydrolase can be defined as the activity for the cellobiose p-nitrophenyl.This activity is being that micromole's nitrophenyl that 7.0 o'clock per minutes discharge is measured at 37 ℃ of following pH values.Found that endo-dextranase component of the present invention does not have the cellobiose hydrolase activity basically.
Endo-dextranase component in the cellulase preparation of the present invention is isolating by multiple purification process at first, promptly comprises according to U.S.4 the reversed-phase HPLC purifying of 435, the 307 rough H.insolens cellulase mixtures that carry out.This method has caused isolating the 43KD endo-dextranase as one-component astoundingly, owing to its extra high endoglucanase activity makes it have beyond thought premium properties.
Except shaker test, the cellulase that is used for the present composition can also be further defined as the enzyme (hereinafter being referred to as " endo-dextranase ") with endoglucanase activity, or having the homologue of the enzyme of endoglucanase activity, these enzymes have the amino-acid sequence of listing among the additional sequence list ID#2.
In this article, term " homologue " is meant the polypeptide by dna encoding, this DNA is under given conditions (as preimpregnation in 5 * SSC, in the solution of the calf thymus DNA of 20% methane amide, 5 * Denhardt ' s solution, 50mM sodium phosphate, pH6.8 and 50 μ g sex change sonications at 40 ℃ of prehybridization 1h, then in the same solution of having added 100 μ M ATP at 40 ℃ of hybridization 18h) hybridize to and be identical the detecting on the thing of endo-dextranase coded DNA with this amino-acid sequence.This term comprises the derivative of said sequence, obtain the C-that these derivatives can be by being added to one or more amino-acid residues natural order and the one or both ends of N-end, on one or more sites of natural order, replace one or more amino-acid residues, on the one or both ends of natural amino acid order or the one or more sites in natural order, eliminate one or more amino-acid residues, or insert one or more amino-acid residues on the one or more sites in natural order.
The production of endo-dextranase of the present invention can be passed through Humicola species such as Humicola insolens, for example strain DSM 1800, according to clause (Budapest agreement) about Budapest agreement of the international recognition of the microbial preservation that is used for the patented procedure purpose, be deposited in Deutsche Sammlung von Mikroorganismen on October 1st, 1981, Mascheroder Weg 1B, D-3300 Braunschweig, FRG.
On the other hand, except shaker test, be used for cellulase of the present invention and can also be further defined as endo-dextranase, or show the homologue of the enzyme (as defined above) of endoglucanase activity with the amino-acid sequence of listing in additional sequence list ID#4.The production of described endo-dextranase can be passed through Fusarium species such as Fusarium oxysporum, for example strain DSM 2672, clause according to Budapest agreement, be deposited in Deutsche Sammlung von Mikroorganismen June 6 nineteen eighty-three, Mascheroder Weg 1B, D-3300 Braunschweig, FRG.
In addition, the homologous endo-dextranase of expection can be by other microorganisms that can produce cellulolytic enzyme, and for example species Trichoderma, Myceliophthora, Phanerochaete, Schizophyllum, Penicillium, Aspergillus and Geotricum derive.
Yet, for the industrial production of cellulase preparation of the present invention, preferably use recombinant DNA technology or other technologies, comprise fermentation adjustment or the sudden change of related microorganism, to guarantee the surplus production of required enzymic activity.These methods and technology are known in the art, and can be implemented at an easy rate by those of skill in the art.
Therefore, the endo-dextranase component can be the enzyme of producing by a kind of method, this method comprises, in the developing medium under the condition that allows expression endo-dextranase component or its precursor, cultivate with recombinant DNA vehicle transformed host cells, this vehicle is being carried the DNA sequence of encode described endo-dextranase component or described endo-dextranase component precursor, and the DNA sequence of encoding function, these functions allow to express the DNA sequence of coding endo-dextranase component or its precursor, and reclaim the endo-dextranase component from culture.
Containing DNA by the DNA sequence of the precursor forms of coding aforesaid endo-dextranase or enzyme forms and comprises that having the DNA that lists in the DNA sequence among additional sequence list ID#1 or the ID#3 forms or its variant.The example of the variant that DNA sequence is suitable is the Nucleotide substituent that does not produce the another kind of amino-acid sequence of endo-dextranase, but this Nucleotide substituent is corresponding to the use of the codon of the host organisms of introducing this DNA composition, or produce the Nucleotide substituent of different amino-acid sequences, therefore this Nucleotide substituent may produce different protein structures, and this protein structure may produce has endo-dextranase mutant of different nature but not natural enzyme.Other examples of possible variant have the end in this order to insert one or more Nucleotide, or eliminate one or more Nucleotide in an end of this order or its.
The DNA that is used for coding endo-dextranase of the present invention forms and can synthesize preparation by established standard method, for example, by S.L.Beaucage and M.H.Caruthers at Tetrahedron Letters 22,1981, phosphamide method described in the PP.1859-1869 or by people such as Matthes at EMBO Journal3,1984, the method described in the PP.801-805.According to the phosphamide method, synthesis of oligonucleotides Nucleotide in automatic dna synthesizer for example purifies it, annealing, connect and carry out vegetative propagation in suitable vehicle.
The separation that the DNA of coding endo-dextranase or its precursor forms can produce the microorganism of cellulase (as Humicola insolens by for example setting up cDNA or foundation, DSM1800) genomic library carries out, and screen positive vegetative propagation by ordinary method, as hybridizing by using oligonucleotide to detect thing, it is (with reference to people such as Sambrook according to standard method that used oligonucleotide is detected thing, Molecular Cloning:A Laboratory Manual, 2nd.Ed.Cold Spring Harbor, 1989) all or part of amino-acid sequence with endo-dextranase is basic synthetic, or by selecting to express the vegetative propagation of suitable enzymic activity (being enzymic activity in the CMC defined above), or vegetative propagation by selecting to produce the reactive protein that has anti-natural cellulose enzyme (endo-dextranase) antibody.
At last, DNA form can be mix synthetic with genomic, mix synthetic with cDNA or mix genomic and origin thing cDNA, this origin thing can be prepared the various piece that this fragment is formed corresponding to whole DNA by connection synthetic, fragment genomic or cDNA origin thing (in appropriate circumstances) according to standard method.DNA forms and can also prepare by using specific releaser to carry out polymerase chain reaction, for example at US4, in 683,202 or people such as R.K.Saiki at Science 239,1988, described in the pp.487-491 like that.
Form the recombinant expressed vehicle of inserting by above-mentioned DNA and comprise any vehicle of being convenient to carry out recombinant DNA method, the host cell that vehicle is introduced into is usually depended in vectorial selection.Therefore, vehicle can be automatic duplicating medium thing, i.e. the vehicle that exists with the outer entity morphology of karyomit(e), and this vectorial duplicating does not rely on THE REPLICATION OF CHROMOSOME, for example plasmid.On the other hand, this vehicle can be when it be introduced into host cell combined entering in the host cell gene group, and be replicated with its combined karyomit(e) that enters.
In this vehicle, the DNA sequence of coding endo-dextranase should be connected on suitable promotor and the terminator codon order feasiblely.This promotor can be any DNA sequence that shows transcriptional activity in the host cell of selecting, and can by the homology of coding host cell or different be that proteinic gene is derived and obtained.The method that is used for connecting the coded DNA order that is respectively endo-dextranase, promotor and terminator codon and is inserted into suitable vehicle for those of skill in the art be known (referring to, people such as Sambrook for example is in the book that is drawn).
Can belong to for example Aspergillus species with above-mentioned DNA composition or above-mentioned expression vehicle transformed host cells, preferably Aspergillys oryzae or Aspergillus niger.The fungal cell can transform by a kind of method, and this method comprises the formation and the conversion of protoplastis, then by carrying out the regeneration of cell walls as known mode certainly.Aspergillus is described in (Novo Industri A/S's) among the EP 238023 as host microorganism, and its content is hereby incorporated by.Host cell can also be a yeast cell, for example
Saccharomyces cerevisiae bacterial strain.
On the other hand, host living beings can be bacterium, particularly Streptomyces and Bacillus and E.coli bacterial strain.The conversion of bacterial cell can be carried out according to ordinary method.For example, at Molecular Cloning:A Laboratory Manual, Cold Spring Harbor is described in 1989 just as people such as Sambrook.
The suitable DNA sequence and the screening of intermedium structure also can be carried out (referring to people such as Sambrook, in the book that is drawn) by standard method.
The medium that is used to cultivate transformed host cells can be any typical media that is suitable for the host cell growth discussed.The endo-dextranase of being expressed can be entered in the developing medium by secretion expediently, and can it be reclaimed from developing medium by currently known methods, these methods comprise by centrifugal or filtration isolates cell from medium, utilize the protein component of salt such as ammonium sulfate precipitation medium, then utilize chromatographic process such as ion exchange chromatography, affinity chromatography etc.
By using recombinant DNA technology, protein purification techniques, fermentation and mutating technology or other technology known in the art of as above explanation, it is possible that highly purified endo-dextranase is provided.
The concentration of above-mentioned cellulase should be such in the present composition, the amount that promptly is released in the zymoprotein in the washing soln be every liter of washing soln 0.005 to 40mg, be preferably every liter of washing soln 0.01 to 10mg.
Optional components
The present composition will comprise optional components usually, and they have generally constituted the part of detergent composition.The example of this optional components has antiredeposition and soil-suspending agent, and white dyes, SYNTHETIC OPTICAL WHITNER, bleach-activating agent, suds suppressor, anti-caking agent, dyestuff and pigment can add them with various amounts as required.
Be applicable to that antiredeposition of the present invention and soil-suspending agent comprise derivatived cellulose, as poly carboxylic acid or its salt of methylcellulose gum, carboxymethyl cellulose and Natvosol and homopolymerization or copolymerization.This base polymer comprises above-mentioned polyacrylic ester and maleic anhydride-acrylic copolymer as washing assistant, and the multipolymer of maleic anhydride and ethene, methylvinylether or methylacrylic acid, and wherein maleic anhydride accounts for multipolymer 20%(mole at least).The consumption of these materials is generally 0.5% to 10%(weight of composition), 0.75% to 8%(weight more preferably), 1% to 6%(weight most preferably).
Preferred white dyes is suitable negatively charged ion, the example has 4,4 '-two-(2-diethanolamino-4-anilino-S-triazine-6-base is amino) Stilbene-2: 2 '-the disulfonic acid disodium, 4,4 '-two-(2-morpholino-4-anilino-S-triazine-6-base is amino) Stilbene-2: 2 '-the disulfonic acid disodium, 4,-4 '-two-(2,4-hexichol amido-S-triazine-6-base is amino) Stilbene-2: 2 '-the disulfonic acid disodium, 4 ', 4 " two-(2; 4-hexichol amido-S-triazine-6-base is amino) Stilbene-2-sulfonic acid-sodium; 4,4 '-two (2-anilino-4-(N-methyl-N-2-hydroxyethylamino)-S-triazines-6-base is amino) Stilbene-2,2 '-the disulfonic acid disodium; 4; 4 '-two-(4-phenyl-2,1,3-triazole-2-yl) Stilbene-2; 2 '-the disulfonic acid disodium, 4,4 '-two-(2-anilino-4-(1-methyl-2-hydroxyethylamino)-S-triazine-6-base is amino) Stilbene-2,2 '-disulfonic acid disodium and 2-Stilbene base-4 " (naphtho--1 '; 2 ': 4; 5)-1,2,3-triazoles-2 " sodium sulfonate.
Can use the inorganic perhydrate SYNTHETIC OPTICAL WHITNER of any particle, its consumption is 3% to a 40%(weight of composition), 8% to 25%(weight more preferably), 12% to 20%(weight most preferably).The preferred embodiment of this SYNTHETIC OPTICAL WHITNER has Sodium peroxoborate-hydrate and tetrahydrate, percarbonate, and composition thereof.
Another kind of preferred blended component separately is the peroxycarboxylic acid bleach precursor, is commonly referred to bleach-activating agent, and it preferably is added into particle or caking form.The example of this suitable compounds is disclosed in English Patent 1586769 and 2143231, and the method that they form particle form is described in the European publication application 0062523.The preferred embodiment of this compounds is tetraacetyl ethylene diamine and 3,5,5-trimethyl acetyl oxygen base benzene sulfonic acid sodium salt.
The general consumption of bleach-activating agent is 0.5% to a 10%(weight of composition), 1% to 8%(weight more preferably), 2% to 6%(weight most preferably).
Another kind of optional components is a suds suppressor, is example with siloxanes and silicon oxide-mixture of siloxanes.Siloxanes is provided by alkylation polysiloxane material usually, and silicon generally uses with form in small, broken bits, for example silica aerogel and xerogel and various hydrophobic silicon.These materials can be added into particle form, and the suds suppressor in these particles is to be bonded in the impervious substantially carrier of on-surface-active detergent of water-soluble or water-dispersion with being convenient to discharge.Use another kind of method, suds suppressor can be dissolved or dispersed in the liquid vehicle, and use by being sprayed on one or more other components.
As mentioned above, useful silicone suds suppressor comprises the alkylation siloxanes of above pointing out type and the mixture of solid oxidation silicon.This class mixture is to prepare by the surface of siloxanes being added to solid oxidation silicon.Preferred silicone suds suppressor is provided by (preferably trimethyl silicone hydride) silicon oxide of hydrophobic silanization, and the granularity of this silicon is in 10 millimicrons to 20 millimicrons scopes, and its specific surface area is greater than 50m
2/ g, it closely mixes with dimethyl silicone fluids, the molecular weight of this siloxanes about 500 to about 200,000 scopes and the weight ratio of the silicon oxide of siloxanes and silanization be about 1: 1 to about 1: 2.
Preferred siloxane foams inhibitor is disclosed in people's such as Bartollota the United States Patent (USP) 3,933,672.Other useful especially suds suppressors are self-emulsifying silicone suds suppressors, and it is described in German patent application DTOS2,646,126(is open on April 28th, 1977) in.An example of this compound is DC-544, can have been bought by Dow Corning on the market, and it is siloxanes/glycol copolymer.
The general consumption of above-mentioned suds suppressor is 0.001% to a 2%(weight of composition weight), preferred 0.01% to 1%(weight).Froth suppressor preferably adds with independent granule form, this just can allow wherein to contain other foam inhibition materials, as C20-C24 lipid acid, the high molecular weight copolymer of microcrystalline wax and oxyethane and propylene oxide, and they have no adverse effect for the dispersiveness of matrix.The technology for preparing this foam inhibition granule is disclosed in people's such as above-mentioned Bartolotta the United States Patent (USP) 3,933,672.
Other useful polymkeric substance are polyoxyethylene glycol, and preferably, molecular weight is 1000 to 10000, more preferably 2000 to 8000, and those polyoxyethylene glycol of 4000 most preferably from about.The consumption of these polymkeric substance is 0.20% to 5%, more preferably 0.25% to 2.5%(weight).The polycarboxylate salt of these polymkeric substance and above-mentioned homopolymerization or copolymerization is of great value for improving retention of whiteness, fabric ash-deposition and improvement clean-up performance to earth, protein dirt and oxidable dirt in the presence of transition metal impurity.
The stain remover that is used for the present composition is the terephthalic acid used always and ethylene glycol and/or propylene glycol unit multipolymer or the terpolymer by various arrangements.The example of this base polymer is disclosed in the United States Patent (USP) 4,116,885 and 4,711,730 and european patent application 0272033 of common transfer.According to EP-A-0272033, particularly preferred polymkeric substance has following formula:
Wherein PEG is-(OC
2H
4) O-, PO is (OC
3H
6O), and T be (pcOC
6H
4CO).
Some polymkeric substance such as molecular weight are generally 5000-20000, and the polyvinylpyrrolidone of preferred 10000-15000 also forms useful reagent, in order to stop the transfer of unstable dyestuff between the fabric in the washing process.
Fabric softener also can add in the detergent composition of the present invention.These reagent can be inorganic type or organic type.Inorganic softening agent is an example with the smectic clays, and it is disclosed in GB-A-1, in 400,898.The organic fabric softening agent comprises the water-insoluble tertiary amine, as be disclosed among GB-A-1514276 and the EP-B-0011340, the combination of these tertiary amines and single C12-C14 quaternary ammonium salt is disclosed among EP-B-0026527 and the EP-B-0026528, and two long-chain acid amides are disclosed among the EP-B-0242919.Other useful organic constituents of fabric sofetening system comprise the high-molecular weight polyethylene oxide, as are disclosed in EP-A-0299575 and 0313146.
The amount of smectic clays is generally 5% to 20%, preferred 8% to 15%(weight), make this material mix the rest part that component is added to preparation to do.The amount that organic softening agent such as water-insoluble tertiary amine or two long-chain acid amides material add is 0.5% to 5%(weight), be generally 1% to 3%(weight), and the amount that high-molecular weight polyethylene oxide material and water-soluble cationic material add is 0.1% to 2%, is generally 0.15% to 1.5%(weight).These materials generally are added to the spraying drying part of composition, but in some instances, preferablyly mix particle they are added to do, or with melt liquid they are sprayed on other solid ingredients of composition.
Enzyme except that the specific cellulase preparation of the present invention may reside in the present composition, as proteolytic enzyme, lipase and amylase.
The preparation method
Composition of the present invention can pass through prepared in various methods, comprises the arbitrary combination of dry blending, spraying drying, agglomeration and granulation and these methods.
Preferred manufacturing procedure
It is combined that the preferred method of the preparation present composition is included in high-speed mixer and the dry blending machine spraying drying and agglomeration.
First particulate component that contains insoluble relatively anion surfactant is by spraying drying, with the rest part remix before part spraying drying product shifted and through the nonionogenic tenside spraying of lower concentration.The preparation of second particulate component is to make neutralizing agent with yellow soda ash, and drying by anionic surfactant acid in continuous high speed mixing machine such as Lodige KM mixing machine neutralizes and carries out.Be fed to first and second components and other dry blending components such as carboxy acid salt chelator, inorganic peroxy SYNTHETIC OPTICAL WHITNER, bleach-activating agent, soil-suspending agent, silicate and enzyme on the travelling belt together then, they are transported to the cylinder that horizontally rotates thus, in cylinder spices and silicone suds suppressor are sprayed on this product.In preferred compositions extremely, adopt another cylinder mixing step, introduce the in small, broken bits crystalline silico-aluminate of lower concentration (being approximately 2%) in this step, to increase density and to improve the particulate flowing property.
Washing methods
The master who is used for washing machine when the present composition of the amount that will be far smaller than common detergent composition washes circulation time, and concentrated cleaning compositions of the present invention can reach identical effect.
Therefore, in another embodiment of the present invention, provide the method for laundering of textile fabrics in washing machine simultaneously, wherein washed circulation for main, the consumption of detergent composition of the present invention is 15 to 170g.
Usually, under the condition in Europe, be used for the main suggestion consumption of washing the round-robin detergent composition and be 80, do not need prewashing to 140g.
Detergent composition of the present invention is preferably directly sent into rotating cylinder rather than the shell by washing machine indirectly.This can realize easy as can that promptly by add composition in bag or reservoir, when cycles of washing began, temperature rise or submergence composition along with stirring, rotating cylinder washed water discharged it thus.This reservoir is placed in the rotating cylinder with the fabric that will wash.Other method is that washing machine itself can be regulated so that composition directly adds rotating cylinder, for example by the levitation device in inlet cover.
Containing the product that is packaged in the detergent composition in bag or the reservoir designs usually by this way, so that whole reservoir is remained under the drying regime, the outflow of thing in the reservoir when preventing drying, but when among being exposed to wash environment, normally being immersed in the aqueous solution, the suitable again thing that discharges its inside of this reservoir.
Reservoir generally is flexible, as bag or box.Bag can have the filamentary structure that has applied fluid-tight protective substance, so that keep the thing in the bag constant, as disclosed in European publication application 0018678.Use other method, bag can be made by the water-insoluble synthetic polymer, and has edge sealing or sealing to be used for the exclusion of water solution medium, as disclosed in European publication application 0011500,0011501,0011502 and 0011968.A kind of simple form of the easy crisp sealing of water comprises the water-soluble binder of one processing and the sealing bag of being made by impermeable polymer film such as polyethylene or polypropylene on one side.
In various bags or reservoir product form, can adopt the lamination sandwich-like product, wherein the center deformable layer is used one or more skins to produce the aesthetic effect of textile-like then with composition dipping and/or coating.These layers can seal together so that in use keep connecting, and perhaps can contact with water respectively so that promote the release of coating or impregnation of matters.
Another kind of lamination form comprises that a mold pressing or deformation layer are used to provide a series of bags of shape reservoirs, and the detergent composition quantitative deposition in each bag, is covered the second layer on the first layer, and those zones between the contacted bag of the sealing two layers shape reservoir.Component is with particle, paste or fusion form deposition, and the lamination layer should stop the material outflow in bag shape reservoir before it is added in the water.These layers can be independently or keep connecting together contacting with water that unique requirement is that this structure should make the interior material snap-out release of bag shape reservoir in solution.The quantity of the bag shape reservoir of per unit area matrix is a selection problem, but usually between every square metre 500 to 25,000.
The suitable material that is used for deformable lamination of the present invention is comprising foam materials, paper and weaving and non-textile.
Yet the preferred method of implementing washing methods of the present invention comprises the reusable dosing unit that has wall of use, and this device can see through liquid but can not see through solids composition.
This class device is disclosed in European patent application publication No. 0343069 and 0344070.A next application discloses a kind of device, and it comprises the bag shape deformable layer that extends from the sustained ring of determining small opening, and small opening wherein can be regulated so that will be used for the enough products of once washing round-robin in cycles of washing and import bag.Part washing medium is flowed in the bag by small opening, dissolves this product, and this solution outwards flows out by small opening and enters washing medium then.Sustained ring is equipped with screening and soaks and the outflow of undissolved product preventing every device, and this device generally comprises the wall that radially extends, and these walls are extended by centre strut with spoke wheel shape or similar structures, and its intermediate interlayer has the spirrillum form.
Embodiment
The following example is for example understood the present invention and is helped to understand the present invention.
The abbreviation of each component has following meaning:
LAS: linear alkyl benzene sulphonate salt
TAS: tallow alcohol sodium sulfate salt
AS:C14-C15 alkylsurfuric acid sodium salt
AO:C12-C14 alkyl dimethyl amine oxide
FA45E7: with the Fatty Alcohol(C12-C14 and C12-C18) (C14-C15) of the ethoxylation of about 7 moles of ethylene oxide
CAT: chlorination C12 alkyl trimethyl ammonium
Clay: smectic clays
Zeolite 4A: the zeolite 4A sodium salt of mean particle size between the 1-10 micron
SKS-6: crystal layer shape silicate (Hoechst)
Copolymer AA/MA: the multipolymer of vinylformic acid and toxilic acid
PAA: molecular weight is the polyacrylic acid of 1000-10000
CMC: carboxymethyl cellulose
Phosphonate: ethylenediamine tetramethylene sodium phosphate salt
EDTA: disodium edta
PB1：NaBO
2·H
2O
2
PB4：NaBO
2·H
2O
2·3H
2O
TAED: tetraacetyl ethylene diamine
NOBS: the ninth of the ten Heavenly Stems acyloxy benzene sulfonic acid sodium salt
P.A.: sulfonation zinc phthalocyanine phthalocyanine
Silicate（R＝n）：SiO
2/Na
2O＝n
Amylase：Termamyl??60T（NOVO-Nordisk）
Lipase：Lipolase??100T（Novo-Nordisk）
Protease：Savinase??4T（Novo-Nordisk）
SSS: press down foam system (silicon oxide-mixture of siloxanes)
The embodiment I
The criticality of claim 1 cellulase performance perameter
Carry out following test:
Test conditions:
Wash temperature: 60 ℃ (giving the circulation heating)
Washing time: 40 minutes
PH＝7.5
The water hardness: 4mmol/L
Detergent concentration: 1%
Detergent composition: referring to EPA350098 embodiment 1
Cellulase:
1) Celluzyme that provides by Novo Nordisk
R=in the same old way
2) 43KD endo-dextranase-cellulase of the present invention
Test-results:
The C14-CMC% that removes by cellulase
The washing composition (=to in the same old way) 0 that does not have cellulase
Washing composition+Celluzyme
R
0.25mg protein/L is lower than 3
0.9mg protein/L 10
1.5mg protein/L 12.7
3.0mg protein/L 17.7
4.5mg protein/L 21.5
Washing composition+43KD endo-dextranase
0.3mg protein/L 20.3
0.25mg protein/L 18.5
Discussion of results:
Last column data clearly illustrates that the criticality of desired cellulase parameter of the present invention has surpassed commercially available Celluzyme.
The embodiment II
Prepare following matrix composition:
Form:
(all concentration are weight %)
The non-concentrated cleaning of concentrated cleaning
LAS??9.40??6.27
TAS??3.00??2.00
FA45E7??2.65??1.77
Trisodium Citrate/citric acid 18.50 12.33
Zeolite??4A??32.65??21.77
Copolymer??AA/MA??4.90??3.27
phosphonate??0.19??0.13
Yellow soda ash 3.00 2.00
Silicate（R＝2）??2.90??1.93
Protease??1.62??1.08
Vitriol 4.50 30.00
SSS??0.40??0.27
Submember+water balance to 100%
(g/L is 680 415 for density
Under 20 ℃)
The product consumption 120 180 of suggestion
The test of (g/ washing) color recovery:
Test conditions:
Bottle formula detergency test instrument
Wash temperature: 40 ℃
Washing time: 3h
The number of times of cycles of washing: 2
The non-concentrated cleaning of PH=8.2
8.5 concentrated cleaning
The water hardness: 15gr./US gal.
Detergent concentration: non-concentrated cleaning is 0.75%
Concentrated cleaning is 0.66%
Test fabric: old blue look pyjamas cotton goods
(90/10 cotton/polyester)
Cellulase: the 1) Celluzyme that provides by Novo Nordisk
R=in the same old way
2) 43KD endo-dextranase=cellulase of the present invention
Washing test: the soiled cotton of the blue look pyjamas fabric that 8g is old is handled with different washing solns.After tumble dried in the rotating cylinder, by directly relatively having two kinds of different detergent base of equal cellulase concentration, the color cleaning effect of fabric is carried out classification.Preferably do the vision classification with 0 to 4 grade by expertise.(0 represents indifference, and 4 represent very big-difference.）
Test-results:
I) non-concentrated cleaning
PSU mg protein/PSU
Cellulose-less enzyme 0
Celluzyme
138mg protein/L+2.3 60
The 43KD endo-dextranase
18.6mg protein/L+2.2 8.5
II) concentrated cleaning
PSU mg protein/PSU
Cellulose-less enzyme 0
Celluzyme
165mg protein/L+3.8 43
The 43KD endo-dextranase
3.4mg protein/L+3.4 1.0
The minimum effectively difference of LSD()=0.5PSU
Calculate following efficiency factor by mg protein/PSU result:
The efficiency factor of 43KD endo-dextranase and Celluzyme ratio:
In non-concentrated cleaning in concentrated cleaning
60/8.5＝7??43/1.0＝43
The efficiency factor of ratio in concentrated cleaning and in non-concentrated cleaning:
The interior dextran of the 43KD of Celluzyme
60/43＝1.4??8.5/1＝8.5
Conclusion:
The above results shows that the efficient of the efficiency ratio prior art cellulase of selected cellulase of the present invention is high 43 times in desired concentrated matrix.In addition, The above results shows, the performance that is obtained by the concentrated matrix of the desired cellulase that is added with selection strengthens unexpectedly to be higher than with the resulting performance of the cellulase of prior art and strengthens.
The embodiment III
Remove contamination tests:
Cellulase also is very effective remove dirt from fabric aspect.This special performances feature is verified by the 43KD endo-dextranase in two kinds of given in embodiment II detergent composition.
Condition:
The Linitest instrument
60 ℃ of washings (giving the circulation heating)
Washing time: 40 minutes
The water hardness: Brussels municipal water
Detergent concentration: concentrated cleaning is 0.66%
Non-concentrated cleaning is 1.0%
Cellulase concentration: 1.55,3.10,4.65 and 6.2mg
Zymoprotein/L washings
Washing test:
The natural clay that obtain of thin cotton fabric with two different areas (US, UK) besmirched.Estimate the performance of cellulase by the clay soil of relatively in two kinds of different detergent composition, washing with the cellulase concentration that equates.Still preferred vision classification level used in the embodiment II.
The result:
Cellulase concentration 1.55 3.1 4.7 6.2
(mg zymoprotein/L
Washings)
Concentrated cleaning
US clay+1.50+2.50+2.00+1.50
UK clay+0.50+1.00+1.50+2.50
Non-concentrated cleaning
(=to in the same old way) 0000
The minimum effectively difference of LSD()=0.42 with 95% degree of confidence
The performance that the selected cellulase of the present invention is removed clay soil in concentrated cleaning compositions of the present invention is higher than the performance of the plain enzyme of identical fibre in common non-concentrated cleaning compositions far away.
Embodiment IV-XI
Also prepared following concentrated cleaning compositions:
Order explanation: SEQ ID NO:1:
Order explanation: SEQ ID NO:2:
Order explanation: SEQ ID NO:3:
Order explanation: SEQ ID NO:4:
Claims (20)
1, a kind of granular detergent composition that contains tensio-active agent, washing assistant and cellulase, it is characterized in that, according to the C14CMC method, said cellulase provides removes at least 10% the radiolabeled carboxymethyl cellulose of immobilization, and this cellulase is with 25 * 10
-6The cellulase protein matter of % (weight) is present in the laundry testing liquid,
Said granular detergent composition contains the inorganic filling salt of no more than 15% (weight), and
The density of said granular detergent composition is that every liter of composition 550 is to 950g.
2, according to the granular detergent composition of claim 1, it is characterized in that, the cellulase compound is made up of similar endo-dextranase component substantially, this similar endo-dextranase component is for causing that anti-partially purified about ≈ 43KD cellulase is (by Humicola insolens, DSM1800 derives) single vegetative propagation antibody have immunoreactivity, or the homologue of said ≈ 43KD endo-dextranase.
3, according to the detergent composition of claim 2, wherein the iso-electric point of the endo-dextranase component of said cellulase is about 5.1.
4, detergent composition according to claim 2 or 3, wherein said endo-dextranase component can be the enzyme of producing by a kind of method, this method comprises, in the developing medium under the condition that allows expression endo-dextranase component or its precursor, cultivate with recombinant DNA vehicle transformed host cells, this vehicle is being carried the DNA sequence of encode described endo-dextranase component or described endo-dextranase component precursor, and the DNA sequence of encoding function, these functions allow to express the DNA sequence of coding endo-dextranase component or its precursor, and reclaim the endo-dextranase component from culture.
5, according to claim 1,2 or 3 detergent composition, wherein the amount of cellulase is such, with the amount that causes the zymoprotein that in washing soln, discharges be every liter of washing soln 0.005 to 40mg, be preferably 0.01 to 10mg.
6, according to the detergent composition of claim 1, wherein said inorganic filling salt is selected from the vitriol and the muriate of basic metal and alkaline-earth metal.
7,, wherein contain no more than 10%(weight according to the detergent composition of claim 1 or 6) inorganic filling salt.
8,, wherein contain no more than 5%(weight according to the detergent composition of claim 7) inorganic filling salt.
9, according to claim 1,2,3 or 6 detergent composition, said composition density is 650 to 850g/L.
10, according to claim 1,2,3 or 6 detergent composition, phosphoric acid salt compound not substantially wherein, and wherein said washing assistant is selected from silico-aluminate ion-exchanger, Citrate trianion, carbonate and composition thereof.
11,, it is characterized in that the cellulase compound is to have the endo-dextranase of listing in the amino-acid sequence among the additional sequence list ID#2, or it shows the homologue of endoglucanase activity according to the granular detergent composition of claim 1.
12, according to the detergent composition of claim 11, wherein said endo-dextranase can be produced by Humicola species such as Humicola insolens.
According to the granular detergent composition of claim 1, it is characterized in that 13, the cellulase compound is to have the endo-dextranase of listing in the amino-acid sequence among the additional sequence list ID#4, or it shows the homologue of endoglucanase activity.
14, according to the detergent composition of claim 11, wherein said endo-dextranase can be produced by Fusarium species such as Fusarium oxysporum.
15, according to the detergent composition of claim 11-14, the DNA of the DNA sequence that wherein said enzyme can be by containing this enzyme of encoding forms and produces.
16, according to the detergent composition of claim 15, DNA sequence wherein is as in the DNA sequence as shown in additional sequence list ID#1 or the ID#3.
17, according to the detergent composition of claim 11-14, wherein said host cell is a fungal bacterial strain, as Tricloderuca or Aspergillus, preferably Aspergillus oryzae or Aspergillus niger, or belong to Hansenula or Saccharomyces bacterial strain, as the yeast cell of Saccharomyces cerevisae bacterial strain.
18, according to the detergent composition of claim 11-14, wherein said host cell is a bacterial isolates, as Bacillus, Streptomyces or E.coli.
19, a kind of in washing machine the method for laundering of textile fabrics, wherein be used for main amount of washing the detergent composition of round-robin claim 1 and be 15 to 170g.
20, according to the method for claim 19 laundering of textile fabrics, wherein, when cycles of washing begins, the detergent composition of described amount is put into the reservoir that can discharge said composition, and said reservoir is put into the washing machine rotating cylinder with the fabric that will wash.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP91870006.3A EP0495344A1 (en) | 1991-01-16 | Compact detergent compositions | |
| EP91870006.3 | 1991-01-16 | ||
| EP91202879.2 | 1991-11-06 | ||
| EP91202879A EP0495257B1 (en) | 1991-01-16 | 1991-11-06 | Compact detergent compositions with high activity cellulase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1064309A true CN1064309A (en) | 1992-09-09 |
| CN1034084C CN1034084C (en) | 1997-02-19 |
Family
ID=26129458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92100955 Expired - Fee Related CN1034084C (en) | 1991-01-16 | 1992-01-16 | Compact detergent compositions with high activity cellulase |
Country Status (13)
| Country | Link |
|---|---|
| JP (1) | JPH06505049A (en) |
| CN (1) | CN1034084C (en) |
| AU (1) | AU662736B2 (en) |
| CA (1) | CA2099508C (en) |
| EG (1) | EG20196A (en) |
| IE (1) | IE920115A1 (en) |
| IN (1) | IN185871B (en) |
| MA (1) | MA22386A1 (en) |
| MX (1) | MX9200171A (en) |
| MY (1) | MY108934A (en) |
| NZ (1) | NZ241308A (en) |
| PH (1) | PH30778A (en) |
| PT (1) | PT100027B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079432C (en) * | 1994-12-28 | 2002-02-20 | 陕西科学院酶工程研究所 | Enzyme preparation special for enzyme washing tertile products and its preparing method |
| CN106232522A (en) * | 2014-04-08 | 2016-12-14 | Pq硅石英国有限公司 | Particulate Zeolite compositions |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2180645T3 (en) * | 1994-06-17 | 2003-02-16 | Genencor Int | CLEANING METHOD BASED ON COMPOSITIONS THAT CONTAIN A CAPABLE ENZYME TO DEGRADE THE CELL WALLS OF THE PLANTS AND ITS USE IN CLEANING METHODS. |
-
1992
- 1992-01-14 MA MA22671A patent/MA22386A1/en unknown
- 1992-01-14 EG EG2092A patent/EG20196A/en active
- 1992-01-14 PH PH43772A patent/PH30778A/en unknown
- 1992-01-15 AU AU15420/92A patent/AU662736B2/en not_active Ceased
- 1992-01-15 MX MX9200171A patent/MX9200171A/en not_active IP Right Cessation
- 1992-01-15 NZ NZ24130892A patent/NZ241308A/en unknown
- 1992-01-15 CA CA 2099508 patent/CA2099508C/en not_active Expired - Fee Related
- 1992-01-15 IE IE920115A patent/IE920115A1/en unknown
- 1992-01-15 JP JP4506431A patent/JPH06505049A/en not_active Ceased
- 1992-01-16 CN CN 92100955 patent/CN1034084C/en not_active Expired - Fee Related
- 1992-01-16 PT PT10002792A patent/PT100027B/en not_active IP Right Cessation
- 1992-01-16 MY MYPI92000070A patent/MY108934A/en unknown
- 1992-01-16 IN IN33DE1992 patent/IN185871B/en unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079432C (en) * | 1994-12-28 | 2002-02-20 | 陕西科学院酶工程研究所 | Enzyme preparation special for enzyme washing tertile products and its preparing method |
| CN106232522A (en) * | 2014-04-08 | 2016-12-14 | Pq硅石英国有限公司 | Particulate Zeolite compositions |
Also Published As
| Publication number | Publication date |
|---|---|
| IE920115A1 (en) | 1992-07-29 |
| IN185871B (en) | 2001-05-12 |
| EG20196A (en) | 1997-10-30 |
| PH30778A (en) | 1997-10-17 |
| MY108934A (en) | 1996-11-30 |
| JPH06505049A (en) | 1994-06-09 |
| MX9200171A (en) | 1993-05-01 |
| CN1034084C (en) | 1997-02-19 |
| AU1542092A (en) | 1992-08-27 |
| PT100027B (en) | 1999-06-30 |
| PT100027A (en) | 1993-05-31 |
| CA2099508A1 (en) | 1992-07-17 |
| AU662736B2 (en) | 1995-09-14 |
| NZ241308A (en) | 1994-07-26 |
| MA22386A1 (en) | 1992-10-01 |
| CA2099508C (en) | 1999-03-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1237162C (en) | Liquid laundry detergent compsns. having enhanced clay removl benefts | |
| US5443750A (en) | Detergent compositions with high activity cellulase and softening clays | |
| CN1094516C (en) | Detergent compositions containing specific lipolytic enzymes | |
| CN1129011A (en) | Detergent composition comprising two cellulase components | |
| CN1738899A (en) | Detergent composition | |
| CN1172498A (en) | Detergent compositions containing lipase and protease | |
| CN1217742A (en) | Detergent compositions comprising improved amylases | |
| CN1140665C (en) | Method for treating polyester fabrics | |
| EP0495258A1 (en) | Detergent compositions with high activity cellulase and softening clays | |
| CN1066878A (en) | Laundry detergent composition | |
| CN1170429A (en) | Cleaning compositions comprising xylanases | |
| CN1120227C (en) | Detergent compositions containing soil release polymers | |
| CN1231689A (en) | Detergent composition comprising two cellulose components, with and without a cellulose-binding domain | |
| CN1374999A (en) | Detergent compositions comprising a retrograded starch degrading enzyme | |
| CN1261400A (en) | Laundry and cleaning compositions containing xyloglucanase enzymes | |
| CN1073476A (en) | Suppress dye transfer compositions | |
| CN1372591A (en) | Detergent compositions comprising an amyloglucosidase denzyme | |
| CN1382204A (en) | Detergent compositions comprising raw starch degrading enzyme | |
| CN1229428A (en) | Detergent compositions comprising improved amylase for dingy fabric clean-up | |
| CN1084559A (en) | Be used to remove the application of the modified poly ester of grease of fabrics | |
| CN1216575A (en) | Cleaning compositions comprising endo-dextranase | |
| CN1034084C (en) | Compact detergent compositions with high activity cellulase | |
| CN1105173C (en) | Dingy fabric clean-up with amylase enzyme in detergent compsns. | |
| CN1225674A (en) | Detergent composition contg. odour removing composition | |
| CN1708584A (en) | Novel cellulase tolerant to surfactants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C15 | Extension of patent right duration from 15 to 20 years for appl. with date before 31.12.1992 and still valid on 11.12.2001 (patent law change 1993) | ||
| OR01 | Other related matters | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |