CN1079432C

CN1079432C - Enzyme preparation special for enzyme washing tertile products and its preparing method

Info

Publication number: CN1079432C
Application number: CN94113376A
Authority: CN
Inventors: 刘涛; 刘芝美; 杨绍琴; 李玉忠; 崔爱荣; 吴江洪; 丁彩霞
Original assignee: ENZYME ENGINEEIRNG INST SHANXI
Current assignee: ENZYME ENGINEEIRNG INST SHANXI
Priority date: 1994-12-28
Filing date: 1994-12-28
Publication date: 2002-02-20
Anticipated expiration: 2014-12-28
Also published as: CN1109504A

Abstract

The present invention relates to a special purpose enzyme preparation and a preparation method thereof, which is used for an enzyme washing textile. The present invention is characterized in that viridin, or flavus, or aspergillus niger is used as strains, and solid fermentation or liquid fermentation is carried out. Then, the strains are extracted and concentrated, and thus, the special purpose enzyme preparation is manufactured. An enzyme preparation A, an enzyme preparation B and an enzyme preparation C can be respectively obtained. The special purpose enzyme preparation can be used for arranging and bleaching cotton textiles, hemp textiles, silk textiles, blended spinning textiles, etc., and can lead the surfaces of the textiles to be smooth and durable. In addition, the textiles have soft hand feel, can be comfortably worn, reduce breaking power and water shrinkage, and improve stiffness, fullness, suspension degree and elasticity. The present invention has the advantages of easy manufacturing, low cost, no toxic pollution and good use effect.

Description

The preparation method of enzyme special for enzyme washing tertile products

The invention belongs to microbial fermentation production zymin technical field, relate to the arrangement and the rinsing of textile fibres article.

Because enzyme is to the enzymolysis of textile fibres primary wall and secondary wall, people have found that enzyme washes arrangement to the enzyme of textile fibres article (cotton, fiber crops, silk, blending etc.), can make the smooth surface of textiles durable, soft, the dress comfortable, for textiles itself, disruptive force and washing shrinkage be can reduce, stiffness, fullness ratio, degree of dangling and elasticity improved.So it has broad application prospect in the arrangement of textile fibres article and the application in the rinsing, however the present production that does not also have this special enzyme preparation.

The object of the present invention is to provide the preparation method of a series of enzyme special for enzyme washing tertile products, make it have the advantage that method is simple, with low cost, result of use is good.

The invention is characterized in, the enzyme special for enzyme washing tertile products is that the black mold bacterium with the Aspergillus flavus of the trichoderma viride of Trichoderma or Aspergillus or Aspergillus is that bacterial classification carries out solid or liquid fermenting, make the solid enzyme that its product can have spissated liquid enzymes and mist projection granulating to form through extracting, concentrating again.Wherein the A zymin that obtains with the trichoderma viride fermentative preparation mainly is a cellulase, and it is to measure its enzymic activity with filter paper sugar method; The B zymin that obtains with the Aspergillus flavus fermentative preparation mainly is a proteolytic enzyme, and it is to measure its enzymic activity with the forint method; The C zymin that obtains with the black-koji mould fermentative preparation is polygalacturonase and cellulase, and it is to measure its enzymic activity with the galactosonic acid method.Feature of the present invention also is:

1. the solid fermentation method of enzyme preparation is,

(1) A zymin: select nontoxic green grass powder for use or/and crop material powder 60-80%

(below be weight ratio), wheat bran 20-40%, other adds ammonium sulfate 1-3%,

SODIUM PHOSPHATE, MONOBASIC (potassium) 0.1-0.4%, sal epsom 0.05%, trisodium phosphate

0.3-0.7%, with above-mentioned material mixing, as many again again water is after the sterilization

Inoculation viride bacterial classification ventilates under 25-32 ℃ of conditions in the ventilation pond and sends out

Ferment 2-4 days;

(2) B zymin: select wheat bran 85-95% for use, soybean cake powder (or bean powder) 5-15%,

And inorganic salts and water in (1), sterilization back inoculation flavus bacterial classification is with (1)

In conditional request ferment;

(3) C zymin: select wheat bran 90-95% for use, pectin classes such as orange meal or persimmon Pi Fen

Material 3-5%, and inorganic salt and water in (1), sterilization back inoculated aspergillus niger

Bacterial classification ferments with the conditional request in (1).

2. the liquid fermentation process of enzyme preparation is,

(1) A zymin: the weight of pressing fermented liquid adds the green grass powder or/and the crop straw

Bar powder 3-5%, wheat bran 2-5%, soybean cake powder (or bean powder) 0.3-1%,

Other adds ammonium sulfate 1-4%, potassium primary phosphate (sodium) 0.3-1%, sal epsom

0.2-0.6%, will sterilize behind the above-mentioned material mixing, inoculation viride bacterial classification,

Ventilating fermentation 2-4 days under 25-32 ℃ of conditions in fermentor tank is in the fermenting process

Keep PH＞3.5;

(2) B zymin: the weight of pressing fermented liquid adds wheat bran 2-5%, soybean cake powder

With Semen Maydis powder each 0.5-2%, peptone 0.5-2%, yeast extract paste 0.2-

0.8%, and inorganic salts in (1), sterilization back inoculation flavus bacterial classification is with (1)

In conditional request ferment;

(3) C zymin: the weight of pressing fermented liquid adds wheat bran 4-8%, orange meal

Or pectic substance 1-4% such as persimmon Pi Fen, and the inorganic salts in (1), go out

Inoculated aspergillus niger bacterial classification behind the bacterium ferments with the conditional request in (1).

3. the enzyme liquid extracting method in the enzyme preparation is, or/and add 1-5% the agent of wadding a quilt with cotton with fixed attention in the liquid fermenting gained vat liquor, treat the post precipitation disgorging at solid fermentation, promptly obtain the extracting solution of zymin, can obtain the liquid enzymes product through concentrating again, or mist projection granulating becomes the solid enzyme product.The used agent of wadding a quilt with cotton with fixed attention is a weight ratio, can select 2-4% polyacrylamide and 1-2% Sodium phosphate dibasic (potassium) for use, or 1-5% Tai-Ace S 150.

The using method of the serial enzymes preparation that the present invention is prepared, the A zymin is mainly used in cotton textiles, the B zymin is mainly used in silk, silk fabric textiles, the C zymin is mainly used in fiber crops, blended textile, and wash the different of purpose with enzyme by the difference of textiles material quality, these several zymins can be used in combination in proportion.Requirement when general enzyme is washed is PH4-5,45-50 ℃ of temperature, and the molten ratio of enzyme 1: 10-50, enzyme is washed 10-100 minutes time kind, all can with stirrer (washing machine) or hand rubbing.Wherein the molten different difference of washing purpose with the different and enzyme of material quality than (enzyme dosage) and the enzyme time of washing of enzyme are very big, can only in use progressively grasp rule.

The enzyme special for enzyme washing tertile products that the present invention is prepared, have make simple, with low cost, to human body toxicological harmless effect, pollution-free, advantage that result of use is good.With the prepared A zymin of the present invention pure cotton canvas is carried out enzyme and wash, its disruptive force descends 17/51% after tested, and washing shrinkage descends 43/66%, and stiffness improves 27%, and fullness ratio improves 34%, and degree of dangling improves 33%, and DE improves 41%.

In technique scheme, preparation process of the present invention is described in detail, because the singularity of industrial fermentation process, its to adding material and the conditional request of preparation be not unusual strictness and accurately, and some process belongs to conventional fermentation, be that this technical field professional is familiar with, so the embodiment in the preparation is repeated no more here.

Claims

1. preparation method who is used for the enzyme special for enzyme washing tertile products, it is characterized in that, be to be that bacterial classification ferments with the mould, its fermentation is to be added with ammonium sulfate, SODIUM PHOSPHATE, MONOBASIC (potassium), sal epsom in fermentation raw material, sterilize behind the mixing, insert the mould bacterial classification, under 25-32 ℃ condition ventilating fermentation 2-4 days, again fermented product is obtained enzyme preparation product through extracting, concentrating, its product can be the solid enzyme that spissated liquid enzymes and mist projection granulating form; Described enzyme bacterium can be any of trichoderma viride, Aspergillus flavus, black-koji mould.

2. preparation method according to claim 1, it is characterized in that, press the weight of fermentation raw material and add 1-3% ammonium sulfate, 0.1-0.4% SODIUM PHOSPHATE, MONOBASIC (potassium), 0.05% sal epsom, 0.3-0.7% trisodium phosphate, as many again again water behind the mixing, bacterial classification is inserted in the sterilization back, carries out solid fermentation in the ventilation pond.

3. preparation method according to claim 2 is characterized in that, fermentation raw material is (weight ratio) green grass powder or/and crop material powder 60-80%, wheat bran 20-40%; The bacterial classification that inserts is a trichoderma viride.

4. preparation method according to claim 2 is characterized in that, its fermentation raw material is (weight ratio) wheat bran 85-95%, soybean cake powder 5-15%; The bacterial classification that inserts is an Aspergillus flavus.

5. preparation method according to claim 2 is characterized in that, its fermentation raw material is (weight ratio) wheat bran 90-95%, orange meal or persimmon skin powder 3-5%; The bacterial classification that inserts is a black-koji mould.

6. preparation method according to claim 1, it is characterized in that, the weight of pressing fermented liquid adds fermentation raw material, and sterilize after adding 1-4% ammonium sulfate, 0.3-1% potassium primary phosphate (sodium), 0.2-0.6% sal epsom, mixing, insert bacterial classification, in fermentor tank, carry out liquid fermenting, keep PH＞3.5 in the fermentation.

7. preparation method according to claim 6 is characterized in that, its fermentation raw material is the green grass powder or/and crop material powder 3-5%, wheat bran 2-5%, soybean cake powder 0.3-1%; The bacterial classification that inserts is a trichoderma viride.

8. preparation method according to claim 6 is characterized in that, its fermentation raw material is a wheat bran 2-5%, soybean cake powder 0.5-2%, Semen Maydis powder 0.5-2%, peptone 0.5-2%, yeast extract paste 0.2-0.8%; The bacterial classification that inserts is an Aspergillus flavus.

9. preparation method according to claim 6 is characterized in that, its fermentation raw material is a wheat bran 4-8%, orange meal or persimmon skin powder 1-4%; The bacterial classification that inserts is a black-koji mould.

10. according to described any preparation method of claim 2-9, it is characterized in that, in resulting enzyme vat liquor, add 1-5% the agent of wadding a quilt with cotton with fixed attention, treat the post precipitation disgorging, promptly obtain the zymin extracting solution, can obtain the liquid enzymes product through concentrating again.