CN106420769A - Application of beta-sitosterol in preparing eimeria tenella resisting drug - Google Patents
Application of beta-sitosterol in preparing eimeria tenella resisting drug Download PDFInfo
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- CN106420769A CN106420769A CN201610680878.3A CN201610680878A CN106420769A CN 106420769 A CN106420769 A CN 106420769A CN 201610680878 A CN201610680878 A CN 201610680878A CN 106420769 A CN106420769 A CN 106420769A
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- Prior art keywords
- sitosterol
- beta
- drug
- cupreol
- anticoccidial
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
- A61K31/575—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
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- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses an application of beta-sitosterol in preparing eimeria tenella resisting drug; the beta-sitosterol and acceptable assistant material are prepared to be dispersing agent, and added into fodder, evenly mixed and fed to chicken according to daily fodder quantity; or the beta-sitosterol is prepared to be beta-sitosterol water soluble powder, dissolved in water and fed to chicken according to daily fodder quantity. Through an animal infection experiment, it can be proved that the anticoccidial index (ACI) of beta-sitosterol can reach 180 above, and beta-sitosterol has good eimeria tenella resisting effect; compared with the regular anticoccidial drug sulfachloropyrazin, the beta-sitosterol has obvious anticoccidial effect. The invention can effectively release the serious drug resistance caused by using anticoccidial drug in current breeding industry; the research and development speed of new drug can be accelerated on this basis; besides, beta-sitosterol has important effect to green and health development of the breeding industry.
Description
Technical field
The present invention relates to a kind of new application of cupreol, be specifically related to cupreol and preparing resisting Eimeria tenella
Application in medicine.
Background technology
Chicken coccidiasis is the main epidemic disease that the intensive poultry husbandry of serious harm produces, the economic damage therefore causing every year
Losing up to more than 2,500,000,000 pounds, its cause of disease is the Eimeria (Eimeria spp) of the multiple door protozoon class in top, wherein harm maximum
Be Eimeria tenella (Eimeria tenella).Chemotherapy is one of important measures of control chicken coccidiasis,
But current Eimeria species has the extensive and serious resistance to the action of a drug, appears on the market in the urgent need to new coccidiostat.
Cupreol (Beta-Sitosterol), also known as (3 β)-beans steroid-5-alkene-3-alcohol, sitosterol, Sitosterolum etc., is somebody's turn to do
The molecular formula of compound is C29H50O, molecular weight 414.71, CAS accession number：83-46-5, shown in its chemical constitution such as formula (I).
Cupreol is distributed widely in plant, can extract, or extract from rice bran oil is got a foothold from several plants oil.?
Attested pharmacological action shows：(1) can be used for medicine, wound healing can be promoted, make hypertrophy, strengthen capillary and follow
Ring.Can be additionally used in the sterol hormone of synthesis regulation water, protein, sugar and inorganic salts, be the important former of production steroid hormone medicine
Material.(2) there is significant anti-inflammatory properties, can be used for treatment cutaneum carcinoma and skin ulcer.There is antioxidant capacity.At suppression sweat gland secretion
Aspect has booster action.With vitamins compatibility for, in skin care item, having conditioning effect, make skin slip moisten, treat or prolong
The keratinization of slow skin.Also can be used as the auxiliary agent for the treatment of.(3) there are norcholesterol, cough-relieving, eliminate the phlegm and suppress tumour and reparation group
Knit effect.It for hypercholesterolemia, atherosclerosis and chronic bronchitis, is also used for pre-term cervical cancer and skin is burst
Ulcer etc..
Cupreol is not yet had to have the report of resisting Eimeria tenella effect so far, known by cupreol
Character cannot infer whether it has the effect of resisting Eimeria tenella.
Content of the invention
It is an object of the invention to provide application in preparing medicament for resisting Eimeria tenella for a kind of cupreol.
The technical solution used in the present invention is：
Application in preparing medicament for resisting Eimeria tenella for the cupreol.
Further, described pharmaceutical dosage form is oral formulations.
Application in preparing feed addictive for the cupreol.
The invention has the beneficial effects as follows：
1) present invention is by by cupreol and acceptable auxiliary material, making powder, adds to feed, mixes, per diem
Normal forage volume feeding chicken, the addition of cupreol is interpolation 5g cupreol in every 100kg feed；Or cupreol is made
Cupreol water solube powder, soluble in water, by everyday water-drinking amount feeding chicken, the addition of cupreol is in every 100kg water
It is dissolved in 2.5g cupreol.Confirm that the anticoccidial index (ACI) of cupreol can reach more than 180 by zoogenetic infection experiment,
There is preferable resisting Eimeria tenella effect.
2) compared with conventional coccidiostat three word coccidia powder, cupreol anticoccidial effect of the present invention is obvious.The present invention
Can effectively alleviate the serious drug resistance problems that current cultivation industry uses coccidiostat to produce, new drug can be accelerated on this basis
Research and development speed, to the green of aquaculture, developing in a healthy way has important benefits.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further illustrated, but is not limited thereto.
Embodiment 1
The preparation of cupreol powder：Take 5g cupreol (content is not less than 95%) and 95g starch mix,.
The usage and dosage of powder：Admix above-mentioned 100g powder in 100kg feed, by daily appetite feeding.
Embodiment 2
The preparation of cupreol water solube powder：Take 2.5g cupreol (content is not less than 95%) and 97.5g glucose
Mix,.
The usage and dosage of water solube powder：It is dissolved in above-mentioned 100g pulvis in 100 liters of water.Cut off the water supply 2 to before medication sick chicken
Hour, then press normal water amount feeding.
Embodiment 3
Anti-Eimeria species experiment below in conjunction with concrete description of test cupreol.
Experiment material：
Control drug：Conventional coccidiostat three word coccidia powder (30% sulfaclozine sodium, lot number 001010), by Shanghai
Animal health Co., Ltd of Novartis produces.
Cupreol：The cupreol powder of embodiment 1, the cupreol water solube powder of embodiment 2.
Coccidian oocyst：Eimeria tenella (Eimeria tenella) Guangdong Strain Sporulated Oocysts, by Guangdong Agricultural
Academy of sciences's veterinary institute preserves, rejuvenation in non-ball worm Chickens before using.
Chick：Lingnan Yellow chick, is provided by Animal Husbandry Inst., Guangdong Prov. Academy of Agricultural Sciences, raises special dynamic in sterilized
In thing room；Chicken coop and the equal strict sterilization of vessel used, free choice feeding and drinking pure water；Observe chick before test and have or not clinical condition
Shape and continuous 2d check that ight soil has or not coccidian oocyst, standby.
Feed：Xin Nan all feed corporation,Ltds in Guangdong Province are customized material of brooding, does not contains any coccidiostat.
Experimental technique：
Packet：It is grouped 150 Lingnan Yellow breeding chicklings to 10 ages in days by test and processes, by only weighing, eliminate weak young bird
Residue chick is randomly divided into 5 groups by the excessive person with body weight, often organizes 30, and suitably adjustment makes often to organize chick TBW substantially phase
Deng.
Process：In addition to I (blank) group, remaining respectively organizes chick equal administered by oral gavage Eimeria tenella Sporulated ovum
Capsule l × 105Individual/plumage；In addition to Group I and the IIth (positive control) group, remaining is respectively organized chick and all feeds immediately after infecting or drink
With the feed containing coccidiostat or water, the IIIth group of feed feeding containing three word coccidia powder, the IVth group of β-paddy feeding embodiment 1
The cupreol spice that sterol powder and feed are made, the Vth group of cupreol water solube powder drinking embodiment 2 and drinking-water
The cupreol drinking-water made, each group drinking-water is all identical with the consumption of feed.The packet situation of test chick is shown in Table 1.
Chick packet tested by table 1
Observe and record the mental status of chicken group, feed intake, ight soil situation etc. every day；Dead chick is weighed, cuts open inspection, if
Death then lesion score is caused to be+4 points for Eimeria Tenella Infection；All chick in infect after the 7th day by only weighing, cut open
Inspection, carries out caecum lesion and scores；Finally calculate weightening finish and the price of deed of each group of chick.
The formula that drug effect decision method and standard anticoccidial index (ACI) press Merck business recommendations calculates：ACI=
(body weight increase rate+survival rate) one (egg capsule value+pathology value)；Body weight increase rate=(test group weightening finish ÷ blank group weightening finish)
× 100%；Survival rate=(each group live chick number ÷ respectively organizes chick sum) × 100%.Pathology is scored by Pyatyi：1. without ovum
Capsule, caecum is normal, is 0 point；2. having egg capsule, caecum mucous membrane slightly thickens, and has and is dispersed in bleeding or a small amount of blood sample intestinal contents on a small quantity, is
+ 1 point；3. having egg capsule, caecum mucous membrane thickens, and has obvious bleeding or obvious blood sample intestinal contents, is+2 points；4. there are egg capsule, caecum
Mucous membrane thickens, and has a large amount of sludged blood or blood sample intestines core, is+3 points；5. chick is because global-worm illness is dead or has a large amount of egg capsule, outside caecum
Seeing in color of soy sauce (or have point-like necrosis region in the middle part of small intestine, mucous membrane face is blush), the obvious enlargement of intestinal tube, content is formed substantially
Blood sample intestines core, be+4 points.In group, pathology value=often is organized average lesion and is scored × 20.Drug effect criterion is ACI<120 is nothing
Effect, 120～160 is weak effect, and 160～180 is middle effect, and more than 180 is potent.Egg capsule value is by cecal content gram egg sac number
(OPG) convert.
Experimental result：
Clinicing symptom observation：
The control group of inoculation Eimeria tenella Sporulated Oocysts the 2nd day, sick chicken occurs that spirit is depressed, slow-witted vertical, appetite
Go down；Within 5th day, obvious bloody stool occur, and death occur, bloody stool gradually decreases subsequently.
Calculate the anticoccidial index of each group respectively, the results are shown in Table shown in 2.As shown in Table 2, the IIIth, the IVth, the anticoccidial of V group
Index is above the IIth group, the IVth, the anticoccidial index of V group apparently higher than the IIIth group, the anti-ball of the real cupreol of historical facts or anecdotes checking
Worm index (ACI) can reach more than 180, has preferable resisting Eimeria tenella effect, and is substantially better than conventional anticoccidial
Medicine three word coccidia powder.
Anticoccidial index respectively organized by table 2
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not by above-described embodiment
Limit, the change made under other any Spirit Essence without departing from the present invention and principle, modifications, replacement, combination, simplification,
All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (3)
1. application in preparing medicament for resisting Eimeria tenella for the cupreol.
2. apply according to claim 1, it is characterised in that：Described pharmaceutical dosage form is oral formulations.
3. application in preparing feed addictive for the cupreol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610680878.3A CN106420769A (en) | 2016-08-17 | 2016-08-17 | Application of beta-sitosterol in preparing eimeria tenella resisting drug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610680878.3A CN106420769A (en) | 2016-08-17 | 2016-08-17 | Application of beta-sitosterol in preparing eimeria tenella resisting drug |
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| CN106420769A true CN106420769A (en) | 2017-02-22 |
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| CN201610680878.3A Pending CN106420769A (en) | 2016-08-17 | 2016-08-17 | Application of beta-sitosterol in preparing eimeria tenella resisting drug |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1741748A (en) * | 2002-11-13 | 2006-03-01 | 巴斯福股份公司 | Pulverulent phytosterol formulations |
| CN1869047A (en) * | 2006-06-28 | 2006-11-29 | 浙江理工大学 | Application of beta sitosterin-3-0 beta D-glucoside-6'-0-eicosanoate as insecticide |
| CN103784428A (en) * | 2013-10-11 | 2014-05-14 | 广东省农业科学院动物卫生研究所 | Application of paeonol in preparing medicine for resisting eimeria tenella |
| CN103931666A (en) * | 2014-04-03 | 2014-07-23 | 福建省农业科学院植物保护研究所 | Insecticide synergist |
-
2016
- 2016-08-17 CN CN201610680878.3A patent/CN106420769A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1741748A (en) * | 2002-11-13 | 2006-03-01 | 巴斯福股份公司 | Pulverulent phytosterol formulations |
| CN1869047A (en) * | 2006-06-28 | 2006-11-29 | 浙江理工大学 | Application of beta sitosterin-3-0 beta D-glucoside-6'-0-eicosanoate as insecticide |
| CN103784428A (en) * | 2013-10-11 | 2014-05-14 | 广东省农业科学院动物卫生研究所 | Application of paeonol in preparing medicine for resisting eimeria tenella |
| CN103931666A (en) * | 2014-04-03 | 2014-07-23 | 福建省农业科学院植物保护研究所 | Insecticide synergist |
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Application publication date: 20170222 |
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