CN106417398A - Method for preventing mosquitoes by using compound Kushen injection dregs - Google Patents
Method for preventing mosquitoes by using compound Kushen injection dregs Download PDFInfo
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- CN106417398A CN106417398A CN201610828090.2A CN201610828090A CN106417398A CN 106417398 A CN106417398 A CN 106417398A CN 201610828090 A CN201610828090 A CN 201610828090A CN 106417398 A CN106417398 A CN 106417398A
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- medicinal residues
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- ethanol solution
- fufang kushen
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for preventing mosquitoes by using compound Kushen injection dregs and an application. The method specifically comprises steps as follows: the compound Kushen injection dregs are dried, crushed and extracted with an ethanol solution, an extract is obtained, after a certain quantity of extracts are put into water, mosquito larva wigglers bred in water can be effectively killed, and the purpose of preventing mosquitoes from spreading diseases is achieved. Traditional herbal medicine dregs are used for preventing the mosquitoes for the first time, and recycling of waste is realized; meanwhile, the method has the advantages of being environment-friendly, safe, convenient to apply and efficient in mosquito killing and the like.
Description
Technical field
The present invention relates to health pest control technology, particularly belongs to a kind of using FUFANG KUSHEN ZHUSHEYE medicinal residues mosquito control
Method.
Background technology
Chinese herbal medicine is that ancestors leave our huge treasure-house for, has long applicating history.In recent years, herbal product with
The features such as its integrally-regulated ability is by force and toxic and side effects are little is increasingly favored by people.Chinese herbal medicine industry is also progressively by extensive
Type production development is scale and modern production, also generates a large amount of medicinal residues while bringing great economic benefit.According to statistics
China's Chinese herbal medicine industry produces medicinal residues every year, and oneself reaches 30,000,000 tons.Traditional medicinal residues processing method includes to burn, fills, fixed area
Domain stacking etc., not only brings great waste, in addition it is also necessary to which enterprise puts into substantial contribution and manpower, while causing serious ring
Border is polluted, and jeopardizes public health.The medicinal residues processing method for falling behind has not met the theory of green production and recycling economy.
Important realistic meaning is recycled to Chinese herbal medicine dregs：It is twice laid first, saves production cost；
Secondly environmental protection, realizes green production；High value-added product can additionally be obtained so that profit maximization.Therefore, Chinese medicine
Enterprise and various circles of society pay attention to the recycling of Chinese herbal medicine dregs further, put into manpower and materials to widen the application of medicinal residues, reach
To the purpose for turning waste into wealth.At present, Chinese herbal medicine dregs can be used for preparing feedstuff, fertilizer, activated carbon etc., but anti-using medicinal residues
The applied research of insect is controlled still in blank stage.
Radix Sophorae Flavescentiss contain matrine and flavonoid chemicals because of which, are often applied in plant insecticide.1999, Zheng Yongquan
Et al. reported first Radix Sophorae Flavescentiss flavone compound there is insecticidal activity.2005, studied by bioactivity screening, find Radix Sophorae Flavescentiss
Middle Multiple components include matrine, oxymatrine, sophocarpine, cytisine etc., all have insecticidal activity to various pests.This is
We develop FUFANG KUSHEN ZHUSHEYE medicinal residues and provide theoretical foundation.
FUFANG KUSHEN ZHUSHEYE also known as Yan-shu injection, the patented product that is researched and developed by Shandong Zhendong Pharmaceutical Co., Ltd.
(the patent No.：200510074841.8), it is the key product of the said firm.The medical instrument has clearing away heat-damp and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body, removing obstruction for relieving pain
The effects such as, the auxiliary treatment of kinds cancer is clinically widely used in, sales situations are good, and development potentiality is larger.Compound light-yellow sophora root is noted
The medicinal residues for penetrating liquid are mainly the dry root of Radix Sophorae Flavescentiss after extracting and the dry rhizome of Smilax lanceaefolia Roxb. Var.opaca A.DC..Due to plant cell wall obstruct and
The limitation of existing extractive technique, remains substantial amounts of effective ingredient in medicinal residues, with the potentiality for developing into green pesticide.
Mosquito class can propagate malaria, dengue fever, yellow fever, filaricide etc. as a kind of Vector, and the serious harm mankind are good for
Health.For a long time, chemical agent is always one of important means of prevention and control mosquito, but unreasonable use so that mosquito generation very
Strong Drug resistance, while also bringing environmental pollution, ecological disruption and threatening the problems such as human health.Vegetable source pesticide medicament
Because its low toxicity, safe and environment-friendly the features such as, increasingly favored by people.As the ovum of mosquito, larva, pupa all live in water
In, periodically eliminated using vegetable source pesticide medicament or control mosquito breeding in water body, not only environmental protection but also can effectively prevent disease
Propagate.
Therefore, the method for researching and developing mosquito control using FUFANG KUSHEN ZHUSHEYE medicinal residues, can reach waste cycling and reutilization
Purpose, and corresponding economic benefit can be produced, also can control pathophoresiss, have the characteristics that achieving many things at one stroke, meet modern life
Produce the theory of green and recycling economy.
Content of the invention
It is an object of the invention to provide a kind of method of utilization FUFANG KUSHEN ZHUSHEYE medicinal residues mosquito control, to reach medicinal residues
While recycling can also mosquito control, effective control pathophoresiss.The method have green safety, apply easy, high
The advantages of imitating parasite killing and recycle.It is a further object of the present invention to provide a kind of extract for anti-middle mosquito of harnessing the river.
A kind of method of utilization FUFANG KUSHEN ZHUSHEYE medicinal residues mosquito control that the present invention is provided, comprises the following steps that：
1) by after the drying of FUFANG KUSHEN ZHUSHEYE medicinal residues, it is put in pulverizer and is crushed；
2) medicinal residues powder is placed in round-bottomed flask, adds the ethanol solution of 7-9 times of volume, reflux, extract, under the conditions of 100 DEG C
1-2 hour；After extracting 2 times, merging filtrate, and be put in revolving bottle and be spin-dried for, that is, obtain extract；
3) by step 2) in the extract obtained water for being directly applied to Mosquito Breeding, reach extract concentrations in water
71.3mg/L～2280mg/L, obtains good insecticide efficiency after 48 hours.
Step 2) in add 8 times of 60% ethanol solution of volume be preferred；Step 2) described in reflux extracting time be 1.5 little
When.Step 3) in preferably pesticidal concentration be 193mg/L, after 48 hours, to can reach more than 98% insecticide efficiency.
A kind of extract for anti-middle mosquito of harnessing the river that the present invention is provided, is obtained by the method for comprising the steps：
1) by after the drying of FUFANG KUSHEN ZHUSHEYE medicinal residues, it is put in pulverizer and is crushed；
2) medicinal residues powder is placed in round-bottomed flask, adds the ethanol solution of 7-9 times of volume, reflux, extract, under the conditions of 100 DEG C
1-2 hour；After extracting 2 times, merging filtrate, and be put in revolving bottle and be spin-dried for, that is, obtain extract.
Described step 2) in ethanol solution be accumulate for medicinal residues powder body 8 times of 60% ethanol solution, addition.
Described step 2) in reflux extracting time be 1.5 hours.
Compared with prior art, a kind of method of utilization FUFANG KUSHEN ZHUSHEYE medicinal residues mosquito control of the present invention, there is provided
A kind of new medicinal residues recycle thinking, have widened the application of medicinal residues, increased enterprise while reducing enterprise's extra charge
The economic benefit of industry, meets the circular economy concept of country's promotion.Additionally, the extract obtained by medicinal residues has low toxicity, environmental protection
And safe the features such as, can be directly applied in the water body of Mosquito Breeding as insecticide, simple to operate and have green prevention and control mosquito concurrently
The feature of worm.
Specific embodiment
The acquisition of 1. FUFANG KUSHEN ZHUSHEYE medicinal residues extract of embodiment
With ethanol solution as the preparation of solvent FUFANG KUSHEN ZHUSHEYE medicinal residues extract：
FUFANG KUSHEN ZHUSHEYE medicinal residues (taking from Shandong Zhendong Pharmaceutical Co., Ltd.) are contained in glass dish, and 55
Dry in DEG C baking oven, weigh mono- group of dry powder 200g after crushed.It is placed in 2000mL round-bottomed flask per group medicinal residues, and adds 7-9
The ethanol solution (10%-80%) of times volume variable concentrations, 100 DEG C of reflux, extract, 1.5 hours, extract 2 times altogether, merging filtrate.
Filtrate is put in revolving bottle and is spin-dried for, the alcohol extracting thing of FUFANG KUSHEN ZHUSHEYE medicinal residues is obtained.Extract quality is shown in Table 1.By
Table 1 understands, the ethanol solution extracted amount highest of 8 times of volumes 60%, is 4.48g.
The quality of FUFANG KUSHEN ZHUSHEYE medicinal residues extract under 1 different volumes concentration ethanol solution condition of table
With water as the preparation of solvent FUFANG KUSHEN ZHUSHEYE medicinal residues extract：
100g medicinal residues dry powder is placed in the round-bottomed flask of 2000mL, adds the deionized water of 7-12 times of volume, 150 DEG C of backflows
Extract 1.5 hours, extract 2 times altogether, merging filtrate.Filtrate is put in revolving bottle and is spin-dried for, the water extract of Radix Sophorae Flavescentiss is obtained.Carry
Take amount of substance and be shown in Table 2.As shown in Table 2,10 times of v aqueous solution extracted amount highests, are 2.91g.
The quality of FUFANG KUSHEN ZHUSHEYE medicinal residues extract under 2 different volumes aqueous conditions of table
| Volume | 7 times | 8 times | 9 times | 10 times | 11 times | 12 times |
| Quality | 1.53g | 1.81g | 2.45g | 2.91g | 2.74g | 2.06g |
As shown in Table 1, FUFANG KUSHEN ZHUSHEYE medicinal residues ethanol extraction optimum condition be 8 times of volumes 60% ethanol molten
Liquid.As shown in Table 2, FUFANG KUSHEN ZHUSHEYE medicinal residues water extract optimum condition is 10 times of v aqueous solution.
2. FUFANG KUSHEN ZHUSHEYE medicinal residues of embodiment and its measure of the extract to the cytotoxicity of Aedes Albopictus Larva.
Aedes albopictus (Aedes albopictus) are a kind of important insect vectors, and at least 26 kinds of propagation is related to the mankind
Virus, cause the infectious disease such as dengue fever, yellow fever, Chikungunya fever and zika virus disease.Meanwhile, it is also that the whole world most enters
One of 100 kinds of biologies of ability are invaded, widely distributed in China.
There is provided by Prevention and Control in Shanxi center friendship for trying Aedes albopictus, in 26 ± 1 DEG C of temperature, relative humidity
70% ± 10%, the photoperiod is L/D：Cultivate under conditions of 12h/12h.
FUFANG KUSHEN ZHUSHEYE medicinal residues and its extract adopt World Health Organization (WHO) to the cytotoxicity of Aedes Albopictus Larva
(WHO) method is supported in the medicinal liquid leaching that recommends.Operation is as follows：
1) add 25mL's to go chlorine water in 50ml beaker, then introduce 20 vigor stalwartnesses, 3 age lineae ablicantes of the same size she
Mosquito larvae.
2) the medicinal residues dry powder after crushing is directly sprinkled into 25mL to go in the small beaker of chlorine water, rocks slightly beaker and cause medicinal residues
Dry powder is uniformly distributed in water.Each concentration is processed and sets 3 groups of repetitions.Matched group does not spread dry powder.
3) extract anhydrous alcohol solution is diluted to the mother solution of variable concentrations, is then added to 25mL and goes the little of chlorine water
In beaker, make dehydrated alcohol final concentration of 1% (v/v), and rock beaker so that extract is uniformly distributed in water slightly.Each
Concentration is processed and sets 3 groups of repetitions.Add the aqueous solution of same volume dehydrated alcohol as a control group.
4) beaker after processing is placed in as described before environment and cultivates 24 hours, not feeding during this.With larva to tweezer
Son touching is not reacted as death standard, statistical experiment result, and fatality rate is meansigma methodss ± SD.
5) data processing.Mortality rate correction is carried out using Abbott formula.Using SPSS13.0 statistical software, data are entered
Row statistical analysiss, calculate extract half lethal concentration (LC50).The fatality rate statistical result in 24 hours of variable concentrations medicinal residues dry powder is shown in
Table 3.The fatality rate statistical result in 24 hours of different concentration ethanol extract is shown in Table 4.It is computed medicinal residues ethanol extraction semilethal dense
Spend for 193mg/L.The fatality rate statistical result in 24 hours of variable concentrations water extract is shown in Table 5.It is computed medicinal residues water extract half to cause
Dead concentration is 55078mg/L.
Table 33 age Aedes Albopictus Larva variable concentrations FUFANG KUSHEN ZHUSHEYE medicinal residues dry powder fatality rate in 24 hours
| Concentration (g/L) | 10 | 20 | 30 | 40 | 50 | 60 |
| Fatality rate (%) | 0 | 0 | 0 | 0 | 0 | 5±5 |
Table 43 age Aedes Albopictus Larva variable concentrations FUFANG KUSHEN ZHUSHEYE medicinal residues ethanol extraction fatality rate in 24 hours
| Concentration (mg/L) | 35.63 | 71.25 | 142.5 | 285 | 570 | 1140 | 2280 |
| Fatality rate (%) | 8.3±3.1 | 18.3±4.6 | 33.3±2.7 | 58.3±1.6 | 86.7±2.9 | 100 | 100 |
Table 53 age Aedes Albopictus Larva variable concentrations FUFANG KUSHEN ZHUSHEYE medicinal residues water extract fatality rate in 24 hours
| Concentration (mg/L) | 2500 | 5000 | 10000 | 25000 | 100000 |
| Fatality rate (%) | 1.3±4.5 | 5.7±5.3 | 20.1±11.8 | 45.2±13.0 | 55.6±11.3 |
5) by FUFANG KUSHEN ZHUSHEYE medicinal residues ethanol extraction according to 193mg/L using dosage, it is added to containing 25mL 20
Going in chlorine water small beaker for 3 age Aedes Albopictus Larvas, arranges 3 groups of repetitions, counts a mortality rate every 8 hours, to obtain second
The time dependence of alcohol extracting thing cytotoxicity.Time dependent fatality rate the results are shown in Table 6.
Fatality rate of the 6 FUFANG KUSHEN ZHUSHEYE medicinal residues ethanol extraction of table to 3 age Aedes Albopictus Larva different times
| Time (hour) | 8 | 16 | 24 | 32 | 40 | 48 | 56 |
| Fatality rate (%) | 12.3±4.9 | 30.8±5.2 | 51.4±3.7 | 74.6±2.1 | 88.3±5.8 | 98.3±4.1 | 100 |
As shown in Table 3, by when in water body, FUFANG KUSHEN ZHUSHEYE medicinal residues dry powder amount is increased to 60g/L its to 3 age lineae ablicantes she
The fatality rate of mosquito larvae is only 5%, and now water body is muddy, does not only reach the purpose of effectively preventing mosquito, and
Return water body and cause certain pollution.Table 4,5 can be calculated half cause in 24 hours of FUFANG KUSHEN ZHUSHEYE medicinal residues ethanol extraction
Dead concentration is 193mg/L；24 hours half lethal concentrations of water extract are 55078mg/L.Water extract is substantially without insecticidal activity.
As shown in Table 6, under the conditions of ethanol extraction half lethal concentration is 193mg/L, the efficiency of mosquito is killed up to 98% after 48 hours
More than.In sum, FUFANG KUSHEN ZHUSHEYE medicinal residues ethanol extraction has good insecticidal effect to wriggler in water.
Measure of the 3. FUFANG KUSHEN ZHUSHEYE medicinal residues ethanol extraction of embodiment to mosquito children cytotoxicity in wild environment.
In order to whether verify FUFANG KUSHEN ZHUSHEYE medicinal residues ethanol extraction to mosquito children in wild environment with cytotoxicity,
Mosquito children wriggler in collection wild environment from the water body of Taiyuan Xiaodian District.After mosquito children's collection, under laboratory condition, 26 ± 1 DEG C of temperature,
Relative humidity 70% ± 10%, the photoperiod is L/D：After 12h/12h is cultivated 24 hours, for the survey of ethanol extraction cytotoxicity
Fixed.After the mosquito of collection is identified, culex pipiens pallens (Culex pipiens pallens) account for mosquito more than 95%, are sociales
Group.
By FUFANG KUSHEN ZHUSHEYE medicinal residues ethanol extraction according to 193mg/L using dosage, the work of head containing 25mL20 is added to
Going in chlorine water small beaker for power healthy and strong, of the same size 3 ages wild mosquito larvae, arranges 3 groups of repetitions, every 8 hours statistics once
Mortality rate, to obtain effect of the ethanol extraction to mosquito children poisoning in wild environment.Ethanol extraction is to mosquito children in wild environment
Fatality rate the results are shown in Table 7.
Fatality rate of the 7 FUFANG KUSHEN ZHUSHEYE medicinal residues ethanol extraction of table to wild mosquito larvae different time
| Time (hour) | 8 | 16 | 24 | 32 | 40 | 48 | 56 |
| Fatality rate (%) | 9.3±4.2 | 25.6±7.3 | 49.3±5.8 | 69.8±8.6 | 84.3±4.8 | 98.1±3.7 | 100 |
As shown in Table 7, under the conditions of ethanol extraction half lethal concentration 193mg/L, to mosquito children in wild environment after 48 hours
Lethal efficiency also up to more than 98%.
By experiment, it was demonstrated that the present invention has very strong toxic action to larvae, can be used for the anti-of larvae
Control.
Claims (7)
1. a kind of method of utilization FUFANG KUSHEN ZHUSHEYE medicinal residues mosquito control, it is characterised in that comprise the following steps:
1) by after the drying of FUFANG KUSHEN ZHUSHEYE medicinal residues, it is put in pulverizer and is crushed；
2) medicinal residues powder is placed in round-bottomed flask, the ethanol solution of 7-9 times of volume is added, under the conditions of 100 DEG C, reflux, extract, 1-2 is little
When；After extracting 2 times, merging filtrate, and be put in revolving bottle and be spin-dried for, that is, obtain extract；
3) by step 2) in the extract obtained water for being directly applied to Mosquito Breeding, make extract concentrations in water reach 71.3mg/L
～2280mg/L.
2. a kind of method of utilization FUFANG KUSHEN ZHUSHEYE medicinal residues mosquito control as claimed in claim 1, it is characterised in that：Step
2) ethanol solution described in is 60% ethanol solution, accumulate for medicinal residues powder body 8 times of addition.
3. a kind of method of utilization FUFANG KUSHEN ZHUSHEYE medicinal residues mosquito control as claimed in claim 1, it is characterised in that：Step
3) concentration of the extract in water is 193mg/L.
4. a kind of method of utilization FUFANG KUSHEN ZHUSHEYE medicinal residues mosquito control as claimed in claim 1, it is characterised in that：Step
2) reflux extracting time described in is 1.5 hours.
5. a kind of for preventing the extract of middle mosquito of harnessing the river, it is characterised in that to be obtained by the method for comprising the steps：
1) by after the drying of FUFANG KUSHEN ZHUSHEYE medicinal residues, it is put in pulverizer and is crushed；
2) medicinal residues powder is placed in round-bottomed flask, the ethanol solution of 7-9 times of volume is added, under the conditions of 100 DEG C, reflux, extract, 1-2 is little
When；After extracting 2 times, merging filtrate, and be put in revolving bottle and be spin-dried for, that is, obtain extract.
6. a kind of for preventing the extract of middle mosquito of harnessing the river as claimed in claim 5, it is characterised in that described step 2) in
Ethanol solution is 60% ethanol solution, accumulate for medicinal residues powder body 8 times of addition.
7. a kind of for preventing the extract of middle mosquito of harnessing the river as claimed in claim 1, it is characterised in that described step 2) in
Reflux extracting time be 1.5 hours.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110326517A (en) * | 2019-07-17 | 2019-10-15 | 长治学院 | A kind of indoor growing matrix and preparation method thereof |
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|---|---|---|---|---|
| CN1209273A (en) * | 1997-08-21 | 1999-03-03 | 李德安 | Pollution-free plant mosquito-fly-killing agent |
| CN104146014A (en) * | 2014-07-18 | 2014-11-19 | 李克锋 | Preparation method for mosquito-expelling traditional Chinese medicine sachet |
-
2016
- 2016-09-18 CN CN201610828090.2A patent/CN106417398A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1209273A (en) * | 1997-08-21 | 1999-03-03 | 李德安 | Pollution-free plant mosquito-fly-killing agent |
| CN104146014A (en) * | 2014-07-18 | 2014-11-19 | 李克锋 | Preparation method for mosquito-expelling traditional Chinese medicine sachet |
Non-Patent Citations (2)
| Title |
|---|
| 巢建国: "《中药资源学》", 31 August 2014, 中国医药科技出版社 * |
| 穆凤扬等: "苦参杀蚊虫活性研究", 《山东工业技术》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110326517A (en) * | 2019-07-17 | 2019-10-15 | 长治学院 | A kind of indoor growing matrix and preparation method thereof |
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Application publication date: 20170222 |