CN106390517B - A kind of control method of mechanical arm type solid-phase extracting instrument - Google Patents
A kind of control method of mechanical arm type solid-phase extracting instrument Download PDFInfo
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- CN106390517B CN106390517B CN201610823504.2A CN201610823504A CN106390517B CN 106390517 B CN106390517 B CN 106390517B CN 201610823504 A CN201610823504 A CN 201610823504A CN 106390517 B CN106390517 B CN 106390517B
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- reversal valve
- injection needle
- liquid storage
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/14—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
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- Chemical Kinetics & Catalysis (AREA)
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
A kind of control method of mechanical arm type solid-phase extracting instrument, mechanical arm type solid-phase extracting instrument includes syringe pump and injection needle, and quantitative liquid storage pipe is provided between syringe pump and injection needle；The control method includes：When the piston in syringe pump being made to be moved at the top of the empty cylinder of syringe pump, make the empty cylinder of syringe pump at least partly in be filled with first fluid；Quantitative liquid storage pipe at least partly stores the solution sucked from injection needle；The pipeline of connection syringe pump and quantitative liquid storage pipe at least partly in be filled with second fluid.The control method of mechanical arm type solid-phase extracting instrument provided by the invention makes mechanical arm type solid-phase extracting instrument not will produce cross-infection.
Description
Technical field
Invention is related to a kind of control method of mechanical arm type solid-phase extracting instrument.
Background technology
In the prior art, solid-phase extracting instrument includes syringe pump and injection needle, and syringe pump includes empty cylinder, piston and push-pull rod,
Empty cylinder is preferably cylindrical drum, the first end connecting line of empty cylinder, another end opening, and piston is placed in empty cylinder, and piston, which is connected to, to be pushed away
Pull rod, when push-pull rod band piston is moved at the top of empty cylinder, injection needle is pumped into from test tube in solution to empty cylinder, works as push-pull rod
When band piston is moved to empty cylinder bottom, the liquid in empty cylinder is discharged in injection needle in SPE columns, due to directly taking out solution
Enter into the empty cylinder of syringe pump, and be discharged from the empty cylinder of syringe pump, it is easy to cause cross-infection.
Invention content
In order to overcome disadvantage existing in the prior art, goal of the invention of the invention to be to provide a kind of mechanical arm type solid phase extraction
The control method for taking instrument, is avoided that cross-infection.
To realize that the goal of the invention, the present invention provide a kind of control method of mechanical arm type solid-phase extracting instrument, mechanical arm
Formula solid-phase extracting instrument includes syringe pump and injection needle, and quantitative liquid storage pipe is provided between syringe pump and injection needle；The controlling party
Method includes：When the piston in syringe pump being made to be moved at the top of the empty cylinder of syringe pump, make at least partly middle note of the empty cylinder of syringe pump
First fluid is entered；Quantitative liquid storage pipe at least partly stores the solution sucked from injection needle；Connect syringe pump and quantitative storage
It is filled with second fluid in the pipeline of liquid pipe.
Preferably, the first fluid liquid.
Preferably, the liquid is water or alcohol.
Preferably, the second fluid is gas.
Preferably, gas is air or nitrogen.
Preferably, by the first reversal valve to injecting liquid in syringe pump, first reversal valve has first port, the
Two-port netwerk and third port, wherein first port is connected to syringe pump, and second port is connected to first fluid holding vessel, third
Port is connected to injection needle.
Preferably, the second reversal valve gives injection gas, the second reversal valve to have first port, second port and third end
Mouthful, wherein first port is connected to the third port of the first reversal valve, and second port is connected to air accumulator or the external world, third end
Mouth is connected to injection needle.
Compared with prior art, the control method of mechanical arm type solid-phase extracting instrument provided by the invention is avoided that intersection sense
Dye.
Description of the drawings
Fig. 1 is the composition schematic diagram for the mechanical arm type solid-phase extracting instrument that first embodiment of the invention provides；
Fig. 2 is the built-up circuit figure of the control system for the mechanical arm type solid-phase extracting instrument that first embodiment of the invention provides；
Fig. 3 is the schematic diagram for the liquid-level tracking principle that first embodiment of the invention provides；
Fig. 4 is the control process flow of the control system for the mechanical arm type solid-phase extracting instrument that first embodiment of the invention provides
Figure；
The control process flow of the pumping liquid for the mechanical arm type solid-phase extracting instrument that Fig. 5 first embodiment of the invention provides
Figure；
The composition schematic diagram for the mechanical arm type solid-phase extracting instrument that Fig. 6 second embodiment of the invention provides；
The built-up circuit figure of the control system for the mechanical arm type solid-phase extracting instrument that Fig. 7 second embodiment of the invention provides.
Specific implementation mode
Technical scheme of the present invention is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation
Example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill
The every other embodiment that personnel are obtained without making creative work, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that term "center", "upper", "lower", "vertical", "horizontal",
The orientation or positional relationship of the instructions such as "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is merely for convenience of retouching
It states the present invention and simplifies description, do not indicate or imply the indicated device or element must have a particular orientation, with specific
Azimuth configuration and operation, therefore be not considered as limiting the invention.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation " " is set
Set ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be directly connected, can also can also be the connection inside two elements indirectly connected through an intermediary, it is right
For those skilled in the art, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
First embodiment
Fig. 1 is the composition schematic diagram for the mechanical arm type solid-phase extracting instrument that first embodiment of the invention provides, as shown in Figure 1,
Mechanical arm type solid-phase extracting instrument provided by the invention can be from the liquid level in motion tracking container to be extracted, and from different appearances
When extracting different solution in device and being injected into SPE columns, cross-infection can be avoided.Mechanical arm type solid-phase extracting instrument includes three axis
Mechanical arm, the three shaft mechanicals arm include orthogonal X arms（X-axis）, Y arms（Y-axis）And Z arms（Z axis）（Do not show in figure）, Z
Arm is the arm perpendicular to ground, can be moved forward and backward along Y arms under motor M3 drivings；Y arms can be under motor M4 drivings along X
Arm moves left and right.Z arm upper edge vertical axials are provided with lead screw 9, sliding block 15 are provided on the lead screw, the sliding block 15 is in motor
It is vertically moved along lead screw 15 under the driving of M2.Mechanical arm type solid-phase extracting instrument further includes liquid level sensor 12, in the present invention
It is preferred that capacitance sensor as liquid level sensor 12, is used to measure the liquid levels height in container, the preferred container of the present invention
For test tube, in the present invention, in holder（Do not show in figure）Upper is in rectangular N*M test tube of setting or SPE columns, N and M be all higher than or
Equal to 2, such as test tube 16, test tube 17, test tube 18 and the SPE columns 19 that are arranged in X direction, each test tube are used to store different molten
Liquid.Mechanical arm type solid-phase extracting instrument further includes injection needle 8, is used to extract the set solution measured from test tube and is injected into
In SPE columns, in SPE columns 19.Mechanical arm type solid-phase extracting instrument further includes syringe pump 1, and the syringe pump 1 passes through pipeline and injection
Needle 8 is connected.Syringe pump 1 includes that empty cylinder, piston and push-pull rod, empty cylinder are preferably cylindrical drum, and one end connecting line of empty cylinder is another
End opening, piston are placed in empty cylinder, and piston is connected to push-pull rod, when push-pull rod band piston is moved to empty cylinder bottom, liquid storage
Liquid in pipe 3 is discharged out of injection needle, and when push-pull rod band piston is moved at the top of empty cylinder, injection needle is pumped into out of test tube
In solution to liquid storage pipe 3.The operation of push-pull rod is driven by motor M1.
In the present invention, a signal lead-out wire 13 of liquid level sensor 12 is connected to injection needle 8, the public affairs of liquid level sensor 12
End is connected to capacitor plate 14 altogether, and the capacitor plate 14 is arranged under test tube, in this way, injection needle 8 and the formation of capacitor plate 14
One capacitance, when injection needle 8 moves down, when touching liquid level, capacitance changes, so as to measure in container
Liquid level, it is generally the case that either lateral cross section is known according to diameter or lateral cross section and liquid to the diameter of container
Face height can calculate the volume of the solution in container, and sectional area is multiplied with liquid level must liquor capacity.
It is provided with pressure sensor 4 at quantitative liquid storage pipe 3 between injection needle and syringe pump, is used to measure quantitative liquid storage
It the pressure of liquid and pressure signal is converted into electric signal then sends control system in pipe 3.
The first solenoid directional control valve 2 is additionally provided in pipeline between injection needle 8 and syringe pump 1, wherein the first electromagnetic switch
The first port of valve 2 is connected to syringe pump 1, and second port is connected to first fluid storage tank, and third port is through the first quantitative storage
Liquid pipe is communicated in the first port of the second solenoid directional control valve, and the first fluid is preferably liquid, more preferably water or alcohol.
First solenoid directional control valve 2 can be such that first port and second port connects under the action of the control signal S4 that control system provides
It passes to from suction water or alcohol.First solenoid directional control valve 2 can make under the action of the control signal S2 that control system provides
First port and third port are connected to so that quantitative liquid storage pipe is connected to through second solenoid valve with injection needle.
The second solenoid directional control valve 5 is additionally provided in pipeline between injection needle 8 and syringe pump 1, wherein the second electromagnetic switch
The first port of valve 5 is connected to the third port of the first solenoid directional control valve 2 through the first quantitative liquid storage pipe, and second port is connected to the
Two fluid tanks, third port are communicated in injection needle 8 through the second quantitative liquid storage pipe 3, and second fluid is preferably gas, and the gas is excellent
It is selected as nitrogen or air；Second solenoid directional control valve 5 can make first under the action of the control signal S3 that control system provides
Port is connected to second port, to inject nitrogen from nitrogen gas tank.The control letter that second solenoid directional control valve 5 is provided in control system
Can also first port be made to be connected to third port under the action of number S3, to be connected to injection needle 8 by the second quantitative liquid storage pipe 3.
In the present invention, it is preferable that injection needle 8 successively by be axially connected with the first overall diameter and first internal diameter the
One steel pipe 81 and the second steel pipe 82 with second external diameter and the second internal diameter, first external diameter is bigger than second external diameter, to be connected
The place of connecing forms step, and second external diameter is slightly less than the first internal diameter, convenient for the first end of the second steel pipe is inserted into the first steel pipe
Second end.Sealing ring 83 is provided at step.The first end of first steel pipe is provided with the first reinforcing sleeve 84, first sleeve 84
It is connected with attachment device 85, attachment device 85 is used to injection needle being connected to sliding block 15.The second end of second steel pipe 81 is formed
Inclined-plane is facilitated insertion into forming needle point in the through-hole of the sealing-plug of solid-phase extraction column.First steel pipe 82 utilizes high strength steel
Standby, the second steel pipe is standby using the white steel of high tenacity, and keeps the outer diameter of the first steel pipe bigger than the outer diameter of the first steel pipe, in this way, making note
It penetrates needle and has both high intensity and high tenacity, to prevent injection needle from fractureing.
In the present invention, solid-phase extraction column（SPE）19 include the sealing-plug of bottle and the bottleneck for sealed bottle body, sealing-plug
Center is axially disposed to have through-hole, the bottle lower end to be provided with liquid receiving mouth, and bottle mouth position is provided with bulge loop.When injection needle penetrate it is close
When through-hole in blocking, by resisting sealing cover when the step of injection needle, the sealing ring 83 on injection needle can just be stuck in sealing cover
In through-hole, play the role of being fully sealed to make sealing ring 83 occur to squeeze.
Fig. 2 is the built-up circuit figure of the control system for the mechanical arm type solid-phase extracting instrument that first embodiment of the invention provides,
As shown in Fig. 2, control system provided by the invention includes：Controller, amplifier, A/D converter 29, the first isolator, second
Isolator, the first driver for driving the first solenoid directional control valve 2 and for driving the second of the second solenoid directional control valve 5 to drive
Dynamic device.
Liquid level sensor 12 is used to track the liquid level information in container, and liquid level information is converted to telecommunications
Breath, is then supplied to controller by phase inverter OP3.Pressure sensor 4 is used to measure the pressure of the liquid in quantitative liquid storage pipe 3
Power, and be power information by the pressure conversion of liquid, then it is supplied to proportional amplifier, proportional amplifier to be used for pressure sensor
The signal of offer is amplified.Illustratively, proportional amplifier includes：Operational amplifier OP1, resistance R5, resistance R6 and resistance
R4, pressure sensor are connected to the reverse side of operational amplifier OP1 through resistance R5, and the output end of operational amplifier OP1 is through resistance
R6 is connected to its inverting input；The in-phase end of operational amplifier OP1 is connected to ground through resistance R4.Operational amplifier OP1's is defeated
Outlet is connected to the input terminal of phase inverter OP2 through A/D converter 21, and the output end of phase inverter OP2 is connected to one of controller
Input terminal, A/D converter are used to the analog signal that proportional amplifier provides being converted to digital signal, and OP2 is used to convert A/D
The signal that device 21 provides carries out reverse phase, and is isolated.
First driver is used to provide drive signal to solenoid directional control valve 2.Illustratively, the first driver includes transistor
TR1, resistance R1 and diode D1, wherein the base stage TR1 of transistor TR1 is connected to controller through resistance R1, and emitter is grounded,
Collector connects the anode of diode D1.The cathode of diode D1 connects power supply, the both ends of diode D1 and the line packet phase of relay J1
It is in parallel.The first end of the power supply of a termination AC220V of the normally opened contact K1 of relay J1, the one of another termination solenoid directional control valve 2
A terminal, another terminal of solenoid directional control valve 2 connect the second end of the power supply of AC220V.When needing solenoid directional control valve 2 to commutate,
Controller provides a high potential signal, transistor turns, the relay J1 being connected with its collector to the base stage of transistor TR1
Line be surrounded by electric current and pass through, normally opened contact is closed, and solenoid directional control valve 2 connects AC220V power supplys and commutates；When needing electricity again
When magnetic reversal valve 2 commutates, controller provides a low-potential signal, transistor cutoff, with its collector to the base stage of transistor TR1
The line packet of connected relay J1 does not have electric current to pass through, and normally opened contact K1 is disconnected, and solenoid directional control valve 2 is disconnected with AC220V power supplys
It opens.
Second driver is used to provide drive signal to the second solenoid directional control valve 5.Illustratively, the second driver includes crystalline substance
Body pipe TR2, resistance R2 and diode D2, wherein the base stage TR2 of transistor are connected to controller through resistance R2, and emitter is grounded,
Collector connects the anode of diode D2.The cathode of diode D2 connects power supply, the both ends of diode D2 and the line packet phase of relay J2
It is in parallel.The first end of the power supply of one termination AC220V of the normally opened contact of relay J2, one of another termination solenoid directional control valve 5
Terminal, the second end of the power supply of another terminal connection AC220V of solenoid directional control valve 5.When needing solenoid directional control valve 5 to commutate,
Controller provides a high potential signal to the base stage of transistor TR2, the relay J2's that transistor TR2 is connected with its collector
Line is surrounded by electric current and passes through, and normally opened contact is closed, and solenoid directional control valve 5 connects AC220V power supplys and commutates；When needing electromagnetism again
When reversal valve 5 commutates, controller provides a low-potential signal, transistor TR2 cut-offs, with its current collection to the base stage of transistor TR2
The line packet of extremely connected relay J2 does not have electric current to pass through, and normally opened contact disconnects, and solenoid directional control valve 5 is disconnected with AC220V power supplys
It opens.
Mechanical arm type solid-phase extracting instrument provided by the invention further includes sound light alarming circuit, illustratively, sound-light alarm electricity
Road includes transistor TR4, resistance R7, resistance R8, loud speaker 30 and lamp LED, wherein the base stage of transistor TR4 connects through resistance R7
It is connected to controller, emitter ground connection, collector connects the cathode of lamp LED.The anode of lamp LED connects power supply through resistance R8.When in pipeline
Pressure when being more than setting value, controller provides a high potential signal to the base stage of transistor TR4, transistor TR4 conducting,
LED shines, 30 sounding of loud speaker.
The mechanical arm type solid-phase extracting instrument that first embodiment of the invention provides further includes CAN bus, and controller passes through bus
Interface 35 is connected in CAN bus.
The control system that first embodiment of the invention provides further includes the stepper motor driving for Driving Stepping Motor M1
Device 21 is connected by CAN interface 20 with CAN bus.Controller provides control through CAN bus to stepper motor driver 21
Signal processed, 21 driving motor M1 of stepper motor driver is rotated forward or reversion, so that injection pump work.More specifically, motor
M1 drives the push-pull rod of syringe pump to be moved up and down in its sky cylinder to be further driven to piston, and medium is pumped into when moving down, to
Medium is discharged when upper mobile, the medium is water, alcohol, nitrogen or air.
Control system further includes the stepper motor driver 23 for Driving Stepping Motor M2, passes through CAN interface
22 are connected with CAN bus.Controller provides control signal, stepper motor driver through CAN bus to stepper motor driver 23
23 driving motor M2 are rotated forward or reversion, to make sliding block 15 be moved up and down along lead screw under the driving of motor M2,15 band of sliding block
Dynamic injection needle 8 moves up and down.As shown in figure 3, in the present invention, the length of lead screw is fixed value, and container is existed in rectangular setting
Holder（Do not show in figure）In, the position of lead screw and holder is fixed, in other words, along short transverse, the initial position A of sliding block 15 with
The position of the bottom C of container is fixed, when sliding block 15 drives injection needle 8 to be moved down along lead screw, the umber of pulse of driving motor M2
It is linearly increased with the distance increase moved down, and step-length of the pulsed drive motor to be moved down with movable slider 15
When moving B` for it is known that when injection needle 8 touches liquid level, i.e., injection needle is moved by B, by injection needle, capacitor plate and therebetween
The value for the capacitance that medium is formed changes, and capacitance sensor detects the variation and the variation of this capacitance is converted into voltage
Signal then provides ratio to amplifier, and amplified signal is supplied to A/D converter 29, A/D converter by proportional amplifier
The analog signal received is converted to digital signal by 29, after being supplied to controller, controller to detect the signal through isolator,
Application program in memory is called, the distance that injection needle moves down can be calculated according to umber of pulse and step-length, sliding block initial position subtracts
It goes to move down apart from height of liquid level inside container is just obtained, container is calculated in the product of the cross section of liquid level and container in container
The volume of interior solution, it can be seen that, liquid level tracks of device provided by the invention can track the liquid level of the liquid in container.
Control system further includes the stepper motor driver 25 for Driving Stepping Motor M3, passes through CAN interface
24 are connected with CAN bus.Controller provides control signal, stepper motor driver through CAN bus to stepper motor driver 25
25 driving motor M3 are rotated forward or reversion, so that Z arms are moved forward and backward along Y arms, further, before and after so that Z arms is carried injection needle
It is moved to above the container of respective column.
Control system further includes the stepper motor driver 27 for Driving Stepping Motor M4, passes through CAN interface
26 are connected with CAN bus.Controller provides control signal, stepper motor driver through CAN bus to stepper motor driver 27
27 driving motor M4 are rotated forward or reversion, so that Y arms can move left and right under motor M4 drivings along X arms, further, make
Y arms carry Z arms, and Z arms carry injection needle 8 and move left and right above the container of institute's corresponding line.
The mechanical arm type solid-phase extracting instrument that first embodiment of the invention provides further includes in regulated power supply, and regulated power supply is used for
Electric energy is provided to each component, switch KH2 and KL2 are set between regulated power supply and AC220V power supplys, is in order to when control signal does not rise
When effect, positive closing power supply.
The mechanical arm type solid-phase extracting instrument that first embodiment of the invention provides further includes memory 11, includes at least ROM
And RAM, ROM for store should program and data, RAM is for storing nonce.Memory 11 is connected by CAM bus interface 34
It is connected in CAN bus.Mechanical arm type solid-phase extracting instrument provided by the invention further includes first communication module 10, for wireless communication
Module, such as WIFI module, bluetooth module are used for controller and carry out Radio Link with host computer, and first communication module 10 is logical
CAM bus interface 33 is crossed to be connected in CAN bus.Mechanical arm type solid-phase extracting instrument provided by the invention further includes the second communication mould
Block 32 is preferably network interface card, and for carrying out wire link with host computer, second communication module 31 is connected by CAM bus interface 32
It is connected in CAN bus.Such host computer can give controller to send instruction by the first and second communication modules, and from control
Data are connect in device.The data that liquid level sensor 12 and pressure sensor are transmitted are packaged framing and pass through communication module by controller
It is sent to host computer, the calculating that host computer is also mutually gone according to these data.
Fig. 4 is the control process flow of the control system for the mechanical arm type solid-phase extracting instrument that first embodiment of the invention provides
Figure, as shown in figure 4, the control process of the control system of mechanical arm type solid-phase extracting instrument includes：
So that the control system of mechanical arm type solid-phase extracting instrument is initialized, and receive the data of host computer by communication module,
Drive signal is provided to the third and fourth motor, three shaft mechanical arms is made to run to specified test tube position；Control system gives second
Motor, which provides drive signal, makes Z arms top shoe 15 decline, when the injection needle 8 that sliding block carries in operation encounters liquid level, liquid level
Sensor sends out a level signal to controller.
Controller receives this signal, provides drive signal to first motor to drive syringe pump 1 to extract liquid, and adjust
The speed that injection needle declines on whole Z axis, is allowed to be consistent with liquid level decrease speed, realizes that liquid level follows.
When syringe pump is drawn into the solution of the set amount of experiment, it can give controller one completion signal, then control slide block
It moving up, sliding block drives injection needle pull-up and control machinery arm runs to specified SPE columns position, then controls Z arm top shoes and move down, with
Injection needle is set to decline the aperture for being inserted into SPE column plungers center, when the sealing ring at injection needle step withstands the aperture on lid,
Syringe pump injects the liquid just drawn from test tube.
Syringe pump also connects pure water or alcohol by the first solenoid directional control valve 2, because the liquid of sucking does not enter syringe pump
（If leading to cross contamination into membership）, liquid can only reach before the first solenoid directional control valve in liquid storage pipe 3（Syringe pump is once inhaled
It is 10ML to enter amount, is had between injection needle and three tunnel switching valves>The liquid storage pipe of 10ml stores the liquid of sucking）If inhaling
The liquid entered does not enter syringe pump, then syringe pump shell in suction can shorten syringe pump due to the not no reciprocating motion of medium
Service life, therefore syringe pump can access pure water or alcohol by the first solenoid directional control valve 2, help to aspirate, play the work of lubrication
With.
Due to, there are large particulate matter, being injected to SPE columns in the liquid of some experiments, flow rate of liquid is too fast may to lead to SPE
Column blocks, so pressure sensor 4 is added in the duct, when pressure is more than setting value, instrument is returned by pressure sensor 4
Signal is alarmed, and safe handling device is reached（Or instrument）Purpose.
The control process flow of the pumping liquid for the mechanical arm type solid-phase extracting instrument that Fig. 5 first embodiment of the invention provides
Figure, as shown in figure 5, the control process of the pumping liquid of mechanical arm type solid-phase extracting instrument includes：
S01：Control machinery arm makes injection needle be moved at the test tube of solution to be extracted；
S02：Valve is changed to first, control signal is provided, make the first port and second port fluid communication of the first reversal valve,
First port and third port fluid is set to block；
S03：Control letter signal is provided to first motor M1, first motor M1 is made to drive the piston in syringe pump to empty cylinder
Top-direction moves, and is pumped into liquid, the liquid is preferably pure water or alcohol；
S04：Control signal is provided to the second reversal valve, the first port of the second reversal valve and second port fluid is made to connect
It is logical, so that first port and third port fluid is blocked, gas tank is so made to connect the second port of the second reversal valve, the gas
It is preferred that nitrogen, since nitrogen has certain pressure, therefore gas is filled into the first quantitative storage of the first and second reversal valves of connection
In liquid pipe；
S05：Control signal is provided to the first reversal valve, the first port of the first reversal valve and third port fluid is made to connect
It is logical, so that first port and second port fluid is blocked；
S06：Control letter signal is provided to first motor M1, first motor M1 is made to drive the piston in syringe pump to empty cylinder
Bottom direction moves, and the bottom of empty cylinder connects the first port of the first reversal valve, and liquid is drained into the first reversal valve and second
In the first quantitative liquid storage pipe being connected between reversal valve, the liquid is preferably pure water or alcohol, is so quantified first
Water and gas column are formed in liquid storage pipe；
S07：Control signal is provided to the second reversal valve, the first port of the second reversal valve and third port fluid is made to connect
It is logical, so that first port and second port fluid is blocked, the pipeline between such syringe pump and injection needle is unimpeded；
S08：Control letter signal is provided to first motor M1, first motor M1 is made to drive the piston in syringe pump to empty cylinder
Top-direction moves, and from injection needle and enters solution, the solution is stored in the second quantitative liquid storage pipe 3；
S08：Control machinery arm makes injection needle be moved at SPE columns；
S10：Control letter signal is provided to first motor M1, first motor M1 is made to drive the piston in syringe pump to empty cylinder
Bottom direction moves, and the solution in the second quantity tube is discharged into SPE columns；
S11：Control machinery arm makes injection needle be moved to waste liquid pool（Do not show in figure）Place utilizes liquid and/or gas pair the
Two quantitative liquid storage pipes 3 and injection needle are cleaned, and the liquid is preferably water or alcohol；
S12：Judge whether to also need to extract other solution, if it is repeat the above steps, is extracted from another test tube
Solution is injected into SPE columns, cleaning, otherwise power cut-off process.
Second embodiment
The composition schematic diagram for the mechanical arm type solid-phase extracting instrument that Fig. 6 second embodiment of the invention provides, if Fig. 6 shows, this hair
The mechanical arm type solid-phase extracting instrument that bright second embodiment provides with first embodiment the only difference is that in second solenoid valve and
Third solenoid directional control valve is provided between liquid storage pipe 3, other compositions are identical, only describe second embodiment and are different from the first embodiment
Part, identical part is no longer repeated.
Third solenoid directional control valve 5 is additionally provided in pipeline between injection needle 8 and syringe pump 1, wherein the first electromagnetic switch
The first port of valve 2 is connected to syringe pump, and second port is connected to water pot or alcohol container, and third port is connected to by the road
The first port of two solenoid directional control valves.First solenoid directional control valve 2, can be under the action of the control signal S2 that control system provides
First port is set to be connected to second port, to extract liquid from fluid reservoir, the preferred water of the liquid or alcohol, when needing to pipe
When road, injection needle, reversal valve are cleaned, syringe pump 1 extracts water or alcohol from water pot or alcohol container.First solenoid directional control valve
2 can also be such that first port is connected to third port under the action of the control signal S2 that control system provides, to take out from container
Solution or note is taken to be put into SPE columns.The first port of second solenoid directional control valve 5 is connected to the first solenoid directional control valve 2 by the road
Third port, second port is connected to nitrogen gas tank, and third port is communicated in the first port of the second solenoid directional control valve；Second electricity
Magnetic reversal valve 5 can be such that first port is connected to second port under the action of the control signal S3 that control system provides, with from
Nitrogen is injected in nitrogen gas tank.Second solenoid directional control valve 5 can also make first under the action of the control signal S3 that control system provides
Port is connected to third port, so that pipeline is connected to injection needle.The first port of third solenoid directional control valve 6 is connected to by the road
The third port of second solenoid directional control valve 5, second port are connected to the external world, and third port is even connected to injection needle through liquid storage pipe 3；
Third solenoid directional control valve 6 can be such that first port and second port connects under the action of the control signal S4 that control system provides
It is logical, to inject air from the external world.Third solenoid directional control valve 6 can also make under the action of the control signal S4 that control system provides
First port is connected to third port, so that pipeline is connected to injection needle.
Fig. 7 is the built-up circuit figure of the control system for the mechanical arm type solid-phase extracting instrument that second embodiment of the invention provides,
As shown in fig. 7, control system and the second embodiment institute for the mechanical arm type solid-phase extracting instrument that third embodiment of the invention provides are not
Be only：Control system further comprises the third driver for driving driving third solenoid directional control valve 6, other composition phases
Together, the part that only description second embodiment is different from the first embodiment, identical part is no longer repeated.
Third driver includes transistor TR3, resistance R3 and diode D3, and wherein the base stage TR3 of transistor is through resistance R2
It is connected to controller, emitter ground connection, collector connects the anode of diode D3.The cathode of diode D3 meets power supply, diode D3
Both ends and the line packet of relay J3 be in parallel.The first of the power supply of a termination AC220V of the normally opened contact K3 of relay J3
End, a terminal of another termination solenoid directional control valve 6, the of the power supply of another terminal connection AC220V of solenoid directional control valve 6
Two ends.When needing solenoid directional control valve 6 to commutate, controller provides a high potential signal, transistor to the base stage of transistor TR3
The line for the relay J2 that TR3 is connected with its collector is surrounded by electric current and passes through, and normally opened contact K3 is closed, and solenoid directional control valve 3 is connected
AC220V power supplys and commutate；When not needing the commutation of solenoid directional control valve 6, controller provides a low electricity to the base stage of transistor TR3
Position signal, transistor TR3 cut-offs, the line packet for the relay J3 being connected with its collector do not have electric current to pass through, normally opened contact K3
It disconnects, solenoid directional control valve 6 is disconnected with AC220V power supplys.
The control of the control process and pumping solution of the mechanical arm type solid-phase extracting instrument that second embodiment of the invention provides
Journey is similar with first embodiment, here, no longer repeating.
In the present invention, due to by making to be implanted sequentially liquid, gas and molten in the liquid storage pipe between syringe pump and injection needle
Liquid and solution are separated using gas, solution are made not enter syringe pump and only be temporarily stored in liquid storage pipe by liquid, and liquid storage pipe
Convenient for being cleaned using liquid or gas, therefore avoid cross-infection.
Above in association with attached drawing, the operation principle of the present invention is described in detail.But those skilled in the art should
Understand, specification is only for interpreting the claims.But protection scope of the present invention is not limited to specification.It is any to be familiar with
In the technical scope that the present invention discloses, the variation or replacement that can be readily occurred in should all be contained those skilled in the art
Lid is within protection scope of the present invention.Therefore, the scope of protection of the invention shall be subject to the scope of protection specified in the patent claim.
Claims (1)
1. a kind of control method of mechanical arm type solid-phase extracting instrument, mechanical arm type solid-phase extracting instrument includes syringe pump and injection needle,
The first reversal valve, the first quantitative liquid storage pipe, the second reversal valve and the second quantitative liquid storage pipe are provided between syringe pump and injection needle,
First reversal valve has first port, second port and third port, wherein and first port is connected to syringe pump, and second
Port is connected to water or alcohol holding vessel, and third port is connected to the 4th of the second solenoid directional control valve through the first quantitative liquid storage pipe
Port, by the first reversal valve to injection water or alcohol in syringe pump；Second reversal valve have first port, second port and
Third port, wherein second port is connected to air accumulator or the external world, and third port is connected to injection through the second quantitative liquid storage pipe
Needle injects nitrogen by the second reversal valve, and the control method includes：
S01：Injection needle is set to be moved at the test tube of solution to be extracted；
S02：Control signal is provided to the first reversal valve, makes the first port and second port fluid communication of the first reversal valve, makes
First port and third port fluid block；
S03：Make first motor that the piston in syringe pump be driven to be moved to the top-direction of empty cylinder, is pumped into water or alcohol；
S04：Control signal is provided to the second reversal valve, makes the third port and second port fluid communication of the second reversal valve, makes
First port and third port fluid block, and the second port gas that gas tank connects the second reversal valve is so made to be filled into connection
In the quantitative liquid storage pipe of the first of first and second reversal valves；
S05：The first port and third port fluid communication for making the first reversal valve make first port and second port fluid hinder
It is disconnected；
S06：Make first motor that the piston in syringe pump be driven to be moved to the bottom direction of empty cylinder, the bottom connection first of empty cylinder is changed
To the first port of valve, water or alcohol are drained into the first quantitative liquid storage pipe；
S07：The first port and third port fluid communication for making the second reversal valve make first port and second port fluid hinder
It is disconnected；
S08：Make first motor that the piston in syringe pump be driven to be moved to the top-direction of empty cylinder, the inhalation solution from injection needle,
The solution is stored in the second quantitative liquid storage pipe；
S08：Control machinery arm makes injection needle be moved at SPE columns；
S10：Control signal is provided to first motor, makes first motor that the piston in syringe pump be driven to be moved to the bottom direction of empty cylinder
Dynamic, the solution in the second quantitative liquid storage pipe is discharged into SPE columns.
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|---|---|---|---|
| CN201610823504.2A CN106390517B (en) | 2016-09-14 | 2016-09-14 | A kind of control method of mechanical arm type solid-phase extracting instrument |
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| CN201610823504.2A CN106390517B (en) | 2016-09-14 | 2016-09-14 | A kind of control method of mechanical arm type solid-phase extracting instrument |
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| CN106390517B true CN106390517B (en) | 2018-08-14 |
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| CN110755883A (en) * | 2019-11-21 | 2020-02-07 | 苏州新仪科学仪器有限公司 | Solid phase extraction device and system |
| CN110764407B (en) * | 2019-11-26 | 2022-12-23 | 苏州新仪科学仪器有限公司 | Pressure control method and system of solid phase extraction column and electronic equipment |
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