CN106387854A - Health-care product composition capable of enhancing immunity - Google Patents
Health-care product composition capable of enhancing immunity Download PDFInfo
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- CN106387854A CN106387854A CN201610726850.9A CN201610726850A CN106387854A CN 106387854 A CN106387854 A CN 106387854A CN 201610726850 A CN201610726850 A CN 201610726850A CN 106387854 A CN106387854 A CN 106387854A
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- ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Chemical compound [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 210000002460 smooth muscle Anatomy 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000011655 sodium selenate Substances 0.000 description 1
- 229960001881 sodium selenate Drugs 0.000 description 1
- 235000018716 sodium selenate Nutrition 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 231100000588 tumorigenic Toxicity 0.000 description 1
- 230000000381 tumorigenic effect Effects 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 229940124549 vasodilator Drugs 0.000 description 1
- 239000003071 vasodilator agent Substances 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a health-care product composition capable of enhancing immunity. The composition is prepared from 100-150 parts by weight of a glossy ganoderma extract, 100-150 parts by weight of paecilomyces hepialid powder, 0.01-0.1 part by weight of sodium selenite and 50-150 parts by weight of L-glutamine. The composition has the significant effect of enhancing immunity. The invention further discloses a preparation method of a preparation containing the composition.
Description
Technical field
The invention belongs to field of health care products is and in particular to a kind of Halth-care composition of strengthen immunity.
Background technology
In traditional Chinese medicine, an immune word appearance is later, sees for 19th century earliest《Immune class side》.From the point of view of its original idea, immunity is just
It is intended to exempt disease harm, ensure the health of human body.From this, motherland's medical science is early to immune Connotation Definition and modern medicine
Phase is basically identical to immune being defined on Spirit Essence.This function embodiment is in immune defense, stable, the immune prison of immunity
Depending on three aspects.
In terms of immunoprophylaxis, it is mainly reflected in the coordinating and unifying of Overall View in theory of traditional Chinese medical science and body.Overall View is recognized
An entirety for human body, human body be naturally also an entirety, naturally day alternates with night, the change of Four seasons change and environment,
Will impact human body physiological function activity, by the self-control of human body itself, with the change of reform of nature circle, realize people with
The unity of opposites of nature, therefore advocate and want acting in accordance with the changes of YIN YANG in four seasons to change in terms of health care, actively maintain putting down of human body and external environment
Weighing apparatus, thus keep the inherent coordinated balance of human body itself.If external environment change is fierce, surmount the adaptability of human body or suitable
Power should be able to decline, then can affect the dysequilibrium of inside of human body and fall ill.Modern medicine be also considered as human body have one complete
Immune system, has panimmunity competent cell with its multiple lymphokine and antibody, ties up under nerve is adjusted with endocrine hormone
Hold the physiological equilibrium of people.Form Neuroendocrine-immunoregulatory network system, play defence and the effect resisting disease.
Stablize aspect in immunity, the equilibrium between yin and yang of the traditional Chinese medical science is theoretical, the related theory of the five internal organs, relationship of promotion and restriction of five elements theory etc. all with immunity
Stablize closely related.Negative and positive under normal circumstances are in the dynamic equilibrium under necessarily " spending ", once " spending " is unbalance, will lead to disease
The generation of disease.Just so-called " An excess of yang leads to disorder of yin ", " An excess of yin leads to disorder of yang ", " predominant yang generating heat ", " An excess of yin brings abort cold syndrome ".The marrow of yin-yang theory
It is " permit hold two ends and with wherein " ", pursue " yin and yang in equilibrium " and " to put down by a definite date ";Traditional Chinese medicine regards human body as one with five
The organic whole that each internal organs centered on dirty influence each other and regulate and control, such as Yangming fu-viscera excess and see the do not feel like eating that stomach is not received, or
See the cough of Lung Qi upward reversal, pant;Internal heat is assigned, kidney Ji waterborne, then coordinating water and fire, on the contrary then breakdown of the normal physiological coordination between the heart and the kidney;" the five internal organs all can again
Make us coughing, non-only lung ";" seeing the disease of liver, knowing the liver disease will be transmitted into the spleen, real spleen in the ban " etc..It can be seen that motherland's medical science is from etiology and pathogenesis, diagnosis
And the everyway such as treatment shows that the five internal organs are related and the dynamic stability of generation-inhibition in five elements inhibition and generation.Between the five-element " high, do harm to, hold and be
System ".Immunologic homeostasis can remove damage, old and feeble, dead cell, and effectively carries out immunoregulation effect, if stable
, then easily autoimmune disease in functional disturbance.
In terms of immunosurveillance, it is mainly reflected in in tumorigenic understanding, traditional Chinese medicine thinks, the formation of tumour is
Body " positive unsaturated vapor ", then caused by " perverse trend crouches it ".When body is affected by some factors or the attacking of exopathogen, cause dirty
The physiological activities such as internal organs, channels and collaterals occur extremely, and when negative and positive of qi and blood balance coordination relation is destroyed, human body will be ill.Perfect
Immune surveillance function is built upon, on sound function of immune system (including non-specific and specific immunity) basis, such as exempting from
Epidemic disease monitors mistake department, then tumour cell exempts from supervision and escapes, such as immune deficient patients or a large amount of using immunodepressant person, because exempting from
Epidemic disease supervision is impaired, and tumour often easily occurs.
Immune immune defense, immunity are stablized and immune surveillance function is also among certain dynamic equilibrium, fits
Degree is advisable.And the immunologic function of body is limited to the impact of inside and outside many factors, maintain dynamically putting down of body's immunity
Weighing apparatus, could keep the health of human body.
Modern society, due to dog-eat-dog, pressure increases, and rhythm of life is more and more faster, and for diet, people often turn round and look at not
Upper reasonably combined, balanced nutritious, cause immunity degradation.In in the period of facing extraneous viral prevalence, such crowd is vulnerable to most
Infection.The one big feature of weak and sickly crowd is exactly that immunity is low, and operating pressure big working clan be especially apparent.This life shape
State is main relevant with study, the work excessively inducement such as fatigue, stress, professional stress.Generally go in for the study the mental of work
Labourer, businessman etc. the most easily enter this state.Modern society's keen competition, fast pace, high pressure, have increased the body and mind of people
Burden;Environmental pollution, bad habits and customs and alcohol drinking patterns etc., for example, eat and drink immoderately, thermophilic cigarette is indulged in excessive drinking, the high food of edible tri,
Lead to be deficient in vitamin and trace element etc., reduce the fitness of people from another point of view, these are all to lead to inferior health
The inducement that state occurs.
Immunopotentiator is also referred to as immunologic adjuvant, is that a class is passed through non-specific approach and improved that body fight is former or microorganism is special
The material of opposite sex reaction.In recent years, at home and abroad scholar is to find characteristic determination, efficient, stable, nontoxic preferable Immune-enhancing effect
Under the effort of agent, the research field of immunopotentiator is constantly widened, and newcomer, novel form constantly find, Exploration of Mechanism is constantly deep
Enter, clinical application range is more and more wider.
Immunopotentiator is classified as follows by raw material:
A mineral matter class, selenium, zinc etc.;
B Chinese herbal medicine class, Radix Angelicae Sinensis, ginseng, glossy ganoderma, Chinese caterpillar fungus, Poria cocos, matrimony vine etc. and its polysaccharide, glucoside and volatile ingredient;
C immunologic adjuvants, propolis etc.;
D microorganism formulation class, BCG vaccine, choleratoxin B subunit etc.;
E vitamins, VitAVitE, vitamin D, vitamin C etc.;
F amino acids, arginine, leucine etc.;
G nucleic acid preparation class, many (gathering) nucleotides, immune ribonucleic acid etc..
Selenium is one of key factor of impact body's immunity, can affect the immunologic function of body from many aspects.Right
The understanding of effect in immunologic function for the selenium, is mainly set about by positive and negative two aspects:Observe selenium deficiency animal immune
The change of function;Mend selenium to selenium deficiency animal, observe the change of Immune Function In Animals.Research finds, the shadow to immunologic function for the selenium
Ring widely complicated although some report results are still variant, but comprehensive relevant information, selenium is to immune effect bag
Include:Selenium participates in cellular immune processes:Selenium can affect propagation and the differentiation of lymphocyte, can promote lymphocytic emiocytosis lymph
The factor (such as interferon, interleukin etc.):Selenium can strengthen the CDCC of lymphocyte;Selenium also can promote specificity humoral to exempt from
Epidemic disease function, makes internal antibody level raise:Selenium improves nospecific immunity effect:Animal experiment study and human body epidemiology are adjusted
Look into and also find, selenium can improve the resistivity to parasite and infectious disease pathogens for the body to a certain extent.
Therefore, the raw material rich in selenium, such as Organic Selenium can all be added in more health products:Se-enriched yeast, selenizing card
Draw glue, selenoprotein, selenium-enriched edible mushroom powder and methylselenocysteine etc., also part inorganic selenium also serves as selenium source replenishers,
Such as sodium selenate and sodium selenite.Sodium selenite is colourless crystallization, soluble in water, is current purposes widest selenium hardening agent,
Supplement human body selenium frequently as selenium source.Although sodium selenite is easily prepared as selenium source replenishers, low cost, the poisonous pair to human body
Reaction and gastrointestinal irritation, and body is low to the degree of absorbing of selenium.Thus have adopting Organic Selenium compared with multi-product, particularly preferably rich
Selenium yeast, which obviates toxicity and the gastrointestinal irritation of sodium selenite, but is same as other biological raw material, Se-enriched yeast is same
There is high cost.
Content of the invention
For solving above-mentioned technical problem, it is an object of the invention to provide a kind of Halth-care composition of strengthen immunity,
Said composition with sodium selenite as selenium source, low cost, se complementary effect is good, enhance immunity definite effect.
The above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of Halth-care composition of strengthen immunity, said composition includes Ganodenna Lucidum P.E 100-150 weight portion, bat
Moth Paecilomyces varioti bacterium powder 100-150 weight portion, sodium selenite 0.01-0.1 weight portion, described composition also includes Glu
50-150 weight portion.
Ganodenna Lucidum P.E is glossy ganoderma hot water extract, and glossy ganoderma (Ganoderma lucidum) is a class macro fungi, belongs to
Basidiomycetes, Polyporus, Ganodermataceae.Recent studies indicate that, the main chemical compositions of glossy ganoderma have polysaccharide, ucleosides,
Furosemide feeding class, sterol, alkaloids and amino acids.
Peacilomyce hepiahi bacterium powder:Have shown that peacilomyce hepiahi bacterium powder through the unit research such as institute of materia medica of China Medical institute
Chemical composition is similar to natural cordyceps, the effect of have Cordyceps sinensis, and also has vasodilator, reduces blood pressure, slows down
Heart rate, increase cAMP, stimulate adrenal gland to generate steroid hormone, suppression platelet aggregation, relaxes blood vessels smooth muscle, calm and anti-frightened
Multiple physiologically actives such as faint.
Sodium selenite:Na2SeO3, molecular weight 172.94, No. CAS:10102-18-8, white crystals or crystalline powder, molten
Yu Shui, insoluble in ethanol.
Glu:C5H10N2O3, molecular weight 146.15, No. CAS:56-85-9, white crystals or crystalline substance powder, can be molten
Yu Shui, odorless, nontoxic, often cook nutritional supplement, blending enriching substance, be the abundantest free amino acid in muscle, account for human body
The 60% of free amino acid total amount.Fasting plasma glutamine concentration is 500-750umol/L.Glutamine is not required ammonia
Base acid, it can be by glutamic acid, valine, isoleucine synthesis in human body.Disease, nutritional status be not good or high-intensity exercise
Etc. under stress situation, body increases to the demand of glutamine.
In the present compositions, sodium selenite is selenium source, by affecting body from many aspects to body Selenium Supplement
Immunologic function, such as cellular immune processes:Selenium can affect propagation and the differentiation of lymphocyte, and lymphocytic emiocytosis can be promoted to drench
Ba Yinzi (such as interferon, interleukin etc.);Selenium can strengthen the CDCC of lymphocyte;Selenium also can promote specificity humoral
Immunologic function, makes internal antibody level raise;Selenium can also improve nospecific immunity effect etc..
But body absorbing and bad to inorganic selenium, if improve simply by the content increasing sodium selenite inhaled
Receive availability, limited by the toxic and side effect of sodium selenite and gastrointestinal irritation, particularly inorganic selenium is not easy in internal organs such as again
In liver kidney, accumulation utilizes, even if Blood Se concentration is higher, the content of human body Selenonic protein still can not be promoted to increase, from Organic Selenium
Cost is again too high.The present inventor finds in the formulation procedures of research health products, when the appropriate Glu of addition
When, the degree of absorbing of sodium selenite greatly improves, and not only increases blood selenium level, increases the internal organs eggs containing selenium such as liver kidney simultaneously
White contents level.
And the present invention contains Ganodenna Lucidum P.E, the health products group of peacilomyce hepiahi bacterium powder, sodium selenite and Glu
Compound also shows excellent immunity humidification under synergy.
Preferably, described composition includes Ganodenna Lucidum P.E 120-150 weight portion, peacilomyce hepiahi bacterium powder 100-
130 weight portions, sodium selenite 0.01-0.05 weight portion, Glu 80-120 weight portion.
As a kind of preferred technical scheme, described composition includes Ganodenna Lucidum P.E 130 weight portion, Paecilomyces hepiali chen
Bacterium powder 120 weight portion, sodium selenite 0.03 weight portion, Glu 100 weight portion.
As another kind of preferably technical scheme, this Halth-care composition includes Ganodenna Lucidum P.E 120 weight portion, bat moth
Paecilomyces varioti bacterium powder 130 weight portion, sodium selenite 0.05 weight portion, Glu 120 weight portion.
A kind of preparation of the Halth-care composition containing strengthen immunity of the present invention, described preparation is by above-mentioned composition and auxiliary material
It is obtained, said preparation can be tablet, pill, capsule, powder or granule.Described auxiliary material needs to carry out often selecting according to formulation
Rule auxiliary material.
Preferably, the preparation of the Halth-care composition of strengthen immunity of the present invention is capsule.
Present invention also offers a kind of method of the preparation of the Halth-care composition preparing strengthen immunity, walk including following
Suddenly:
Get the raw materials ready:Weigh Ganodenna Lucidum P.E, peacilomyce hepiahi bacterium powder, Co-60 irradiation sterilization, 4kGy;Difference powder after irradiation
Broken mistake 80 mesh sieve, standby;Sodium selenite, microcrystalline cellulose, starch, silica material pulverized 80 mesh sieves respectively, standby;
Mixing:Sodium selenite is mixed with starch equal increments method, obtains mixed powder, mixed powder is extracted with glossy ganoderma
Mixing in mixer put in the lump by the materials such as thing, peacilomyce hepiahi bacterium powder, microcrystalline cellulose, silica and magnesium stearate
40min, makes material mix, and obtains total mixed powder;
Capsule is filled:By mixed material, automatic capsule filling machine is filled with, operating condition:18~26 DEG C, relatively
Humidity:45%~60%.
The beneficial effects of the present invention is:The Halth-care composition of the present invention selects Ganodenna Lucidum P.E, Paecilomyces hepiali chen
Bacterium powder, sodium selenite and Glu compatibility, beat all are occurring without sodium selenite toxic and side effect and gastrointestinal irritation
In the case of, greatly increase body and selenium is absorbed, not only improve blood Se content moreover it is possible to improve body internal organs Selenonic protein
Level, and show excellent strengthen immunity effect in functional experiment, the present invention strengthens the health products of body immunity
Composition has low cost, the good advantage of effect.
Specific embodiment
Below by way of specific embodiment, the above of the present invention is described in further detail.But should be by
This is interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to Examples below.All technology realized based on the above of the present invention
Belong to the scope of the present invention.
Ganodenna Lucidum P.E is purchased from Sanjiang Biologica Engineering Co., Ltd., Xi-an City;Peacilomyce hepiahi bacterium powder is purchased from Zhejiang Wan Feng and looks forward to
Pharmaceutical Co. Ltd of industry group.
Embodiment 1
Table 1 is filled a prescription
Weigh Ganodenna Lucidum P.E, peacilomyce hepiahi bacterium powder, Co-60 irradiation sterilization, 4kGy by formula ratio;After irradiation respectively
Pulverize, cross 80 mesh sieves, standby.Sodium selenite, microcrystalline cellulose, starch, silica material pulverized 80 mesh sieves respectively, standby
With.
Mixing:Sodium selenite is mixed with starch equal increments method, obtains mixed powder I.Mixed powder I is extracted with glossy ganoderma
Mixing in mixer put in the lump by the materials such as thing, peacilomyce hepiahi bacterium powder, microcrystalline cellulose, silica and magnesium stearate
40min, makes material mix, and obtains mixed powder II.
Capsule is filled:By mixed material, automatic capsule filling machine is filled with, every 0.4g containing content, operation
Condition:18~26 DEG C, relative humidity:45%~60%.
Capsule polishes:Canned capsule is polished on capsule polisher, eliminates surface medicinal powder.
Embodiment 2
Table 2 is filled a prescription
Filled a prescription according to table 2 and prepare product according to embodiment 1.
Embodiment 3
Table 3 is filled a prescription
Filled a prescription according to table 3 and prepare product according to embodiment 1.
Embodiment 4
Table 4 is filled a prescription
Filled a prescription according to table 4 and prepare product according to embodiment 1.
Comparative example 1
Table 5 is filled a prescription
Filled a prescription according to table 5 and prepare product, every 0.4g containing content according to embodiment 1.
Embodiment 5 strengthen immunity function test
1.1 sample:Function is carried out according to the sample (being referred to as sample 1 and sample 2) of embodiment 1 and comparative example 1 preparation
Learn test.Sample is put shady and cool dry and comfortable ventilation and is preserved.People is administered orally and recommends consumption for everyone (adult's body weight is pressed 60kg and calculated) daily 6
Grain, that is, 0.04g/kg BW is daily.Capsule 's content is taken to be tested.
1.2 animals used as test and packet:The cleaning grade breeding breeding from Institute of Experimental Animals, Chinese Academy of Medical Sciences is good for
Health Male Kunming strain mice 210, body weight is 18 22 grams, credit number:SCXK- capital 2004-001.It is divided into 3 groups, every group
70, immune I group, carry out the mouse spleen lymphocyte transformation experiment of ConA induction;Immune II group, carries out delayed allergy
Experiment;Immune III group, carries out serum hemolysin and measures and antibody-producting cell number mensure.
1.3 experimental situation conditions:Animal used as test room temperature:22 25 DEG C, relative humidity:55 70%.
1.4 dosage choice and tested material give mode:It is administered orally according to people and recommends consumption, each sample sets basic, normal, high dosage
Group, respectively 0.20g, 0.40g, 1.20g/kg/ day (are respectively equivalent to 5,10,30 times of human body recommendation consumption), if a moon
Property control group, every group of 10 animals.Tested material is prepared with water, orally administration once a day, and continuous gavage is surveyed items and exempted from after 33 days
Epidemic disease index.Mouse stomach volume is 0.1mL/10g mouse weight.Negative control group replaces tested material with water.
1.5 key instruments and reagent:Animal platform balance, electronic analytical balance, centrifuge, clean bench, carbon dioxide training
Foster case, constant water bath box, microscope, semi-automatic biochemical analyzer, ELIASA etc..
Sterile surgical instrument, micro syringe, cell counter, the flat Tissue Culture Plate in 24 holes and 96 holes, 96 holes are U-shaped thin
Born of the same parents' culture plate, glass dish, gauze, slide, test tube, 200 eye mesh screens, timer hemoglobin pipet etc..
Sheep red blood cell (SRBC) (SRBC), physiological saline, Hank's liquid (pH 7.2 7.4), RPMI1640 nutrient solution, little ox blood
Clearly, penicillin, streptomysin, ConA, 1% glacial acetic acid, the HCL solution of 1mol/L, isopropanol, MTT, DNFB, PBS
(pH7.2 7.4), complement (GPS), SA buffer solution, agarose, YAC-1 cell, lithium lactate, nitro tetrazolium chloride,
PMS, NAD, the Tris-HCL buffer solution of 0.2mol/L, 1% NP40, india ink,
The sodium carbonate of 0.l%, chicken red blood cell, methyl alcohol, Gimsa dye liquor etc..
1.6 experimental technique
1.6.1ConA the mouse spleen lymphocyte transformation experiment (mtt assay) inducing:
Aseptic take spleen, be placed in and fill in right amount in the plate of aseptic Hank's liquid, make cell suspension, through 200 eye mesh screen mistakes
Filter.Washed 2 times with Hank's liquid, centrifugation l0min (1000r/min) every time.Then cell is suspended in 1mL complete culture solution,
Living cell counting number, is 3*10 with RPMI1640 nutrient solution adjustment cell concentration6Individual/mL..Again cell suspension is divided holes to add
In 24 well culture plates, every hole 1mL, hole addition 75uL ConA liquid (being equivalent to 7.5uL/mL) wherein, another hole is as right
According to putting 5%CO2, in 37 DEG C of carbon dioxide incubators, cultivate 72h.Culture terminates front 4h and gently sucks supernatant 0.7mL, adds
0.7mL does not contain the RPMI1640 nutrient solution of calf serum, is simultaneously introduced MTT (5mg/mL) 50uL/ hole, continues culture 4h.Culture
After end, every hole adds 1mL acid isopropyl alcohol, and piping and druming mixes, so that purple crystal is completely dissolved, be then dispensed into 96 well culture plates
In, 3 parallel holes are made in each hole, with ELIASA, measure OD value with 570nm wavelength.The multiplication capacity of lymphocyte is with adding
The OD value in ConA hole deducts and is not added with the OD value in ConA hole and represents.
1.6.2 delayed allergy (DTH) (the sufficient sole of the foot thickens method)
After mouse peritoneal injection 2% hematocrit SRBC (the every mouse of 0.2mL/) sensitization 4 days, measure left back sufficient sole of the foot thickness, then
Measuring point hypodermic injection 20% (v/v) SRBC (the every mouse of 20ul/), after injection, 24h measures left back sufficient sole of the foot thickness, same position
Put measurement three times, average, the degree of DTH is represented with sole of the foot thickness difference (pedal swelling degree) sufficient before and after attacking, tested
The difference of sample sets is significantly higher than the difference of control group, can determine that this experimental result positive.
1.6.3 antibody-producting cell detection (Jerne improves slide method)
Take sheep blood, with brine 3 times, centrifugation l0min (2000r/min) every time, with physiological saline by hematocrit
SRBC is made into the cell suspension of 2% (v/v), every mouse lumbar injection 0.2mL.By the immunity sacrifice of latter 5 days, take spleen, gently
Grind, make cell suspension with Hank's liquid, 200 mesh sieve net filtrations, washing, centrifugation 2 times, finally cell is suspended in 5mL
In Hank's liquid.After top layer culture medium (lg agarose add distilled water to l00mL) heating for dissolving, put 45~50 DEG C of water-baths and protect
Temperature, is mixed with Hank ' the s liquid of 74,2 times of concentration of equivalent pH7.2, dispenses small test tube, often pipe 0.5mL, then plus uses SA into pipe
The 10%SRBC50uL (v/v) of liquid preparation, 25uL splenocyte suspension, are poured into the slide of brush agarose thin layer after rapid mixing
On, to be solidified after, flat for slide button is placed in glass frame, puts in CO2gas incubator incubation 1.5h, dilute with SA buffer solution
The complement (1 released:8) add in glass frame groove, continue incubation 1.5h, count hemolysis plaque number.With plaque number/full splenocyte table
Show antibody-producting cell number.The plaque number of test sample group is significantly higher than the plaque number of control group, can determine that this experimental result
Positive.
1.6.4 the mensure (HC50) of half hemolytic value
Take sheep blood, with brine 3 times, centrifugation l0min (2000r/min) every time, with physiological saline by hematocrit
SRBC is made into the cell suspension of 2% (v/v), every mouse lumbar injection 0.2mL.Immunity 5 days after, extract mouse eyeball, take blood in
In centrifuge tube, place about 1h, 2000r/min is centrifuged l0min, separate, collect serum.With SA buffer solution by serum-dilution be 300
Times, take 1mL to put in test tube, sequentially add 10%SRBC 0.25mL, complement 1mL.Separately set the control tube of not increase serum, delayed with SA
Rush liquid to replace.After being placed in insulation 15min in 37 DEG C of waters bath with thermostatic control, ice bath terminating reaction.2000r/min is centrifuged 10min, takes supernatant
1mL, plus Dou Shi reagent 3mL.Take 10% SRBC 0.25mL simultaneously, plus Dou Shi reagent is to 4mL, in another test tube, fully mixed
Even, after placing 10min, at 540nm, control tube makees blank pipe, measures each pipe OD value respectively.The amount of hemolysin is molten with half
Blood number (HC50) represents, is calculated as follows:
OD value * extension rate during sample half hemolytic value=sample OD value/SRBC HD50
The HC50 of test sample group is significantly higher than the HC50 of control group, can determine whether that experimental result is positive.
1.7 experimental data statistics:Application SPSS statistical software carries out variance analysis statistical disposition.
2. result
The impact to mouse cell immunologic function for 2.1 samples
2.1.1 the impact to mouse delayed allergy (DTH) for the sample
The impact to mouse delayed allergy (DTH) for table 6 sample
Note:* compare with negative control group, P<0.05;* is compared with negative control group, P<0.01.
As seen from the above table, the sample of orally administration mouse various dose is after 33 days, the swelling degree of the paw difference of each dosage group
It is all higher than negative control group, but only sample 1 reaches significant difference, and middle high dose group difference has very significant (P
<0.01), each dosage group difference of sample 2 does not all have conspicuousness (P>0.05).
The impact to lymphocyte transformation test for 2.12 samples
The impact to lymphocyte transformation test for table 7 sample
Note:* compare with negative control group, P<0.05;* is compared with negative control group, P<0.01.
As seen from the above table, each dosage group of sample 1 and 2 is compared with negative control group, and lymphopoiesis ability increases,
But sample 2 no significant difference (P>0.05), only sample 1 shows the significance difference opposite sex (P<, and high dose group otherness 0.05)
Highly significant, illustrates that sample 1 has lymphopoiesis, the effect of conversion capability promoting mouse.
The impact to mouse humoral immune function for 2.2 samples
2.2.1 the impact to mouse antibodies cellulation for the sample
Table 8 sample is to mouse antibodies cellulation test experience result
Note:* compare with negative control group, P<0.05;* is compared with negative control group, P<0.01.
As seen from the above table, each dosage group of sample 1 and 2 is compared with negative control group, and hemolysis plaque number increased, but sample
Low dose group no significant difference (P in 2>0.05), and all dosage groups of sample 1 and sample 2 high dose group show significant difference
Property (P<0.05), particularly sample 1 high dose group, its difference has very significant (P<0.01).
2.2.2 the impact to mouse half hemolytic value (HC50) for the sample
The impact to mouse half hemolytic value (HC50) for table 8 sample
Note:* compare with negative control group, P<0.05;* is compared with negative control group, P<0.01.
As seen from the above table, each dosage group of sample 1 and 2 is compared with negative control group, and sample half hemolytic value increased, but
The each dosage group of sample 2 all no significant difference (P>0.05), and all dosage groups of sample 1 all show significance difference the opposite sex (P<
0.05), particularly sample 1 high dose group, its difference has very significant (P<0.01).
Above-mentioned functions test proves that the Halth-care composition of the present invention has the function of definite strengthen immunity, and because
For the synergy of Glu, compared with the composition of comparative example or negative control group, all there is unexpected effect
Really.
Embodiment 6 rat absorbs test to sodium selenite
Serum selenium can reflect the absorption situation of the recent selenium of body, and selenium is main in body as the necessary trace element of animal
To be played a role in the form of selenoprotein (as containing selenocystein), glutathione peroxidase (GSH-PX) is that research is more
One of selenoprotein, and the expression of selenoprotein and activity all adjust by Se nourishment level;GSH-PX be mammal content
Abundant selenoprotein, its activity is adjusted by the selenium level in animal viscera, and blood selenium can add GSH-PX vigor as biology
With the index utilizing.
1.1 materials and methods
1.1 experimental animal:Breed the SPF level healthy SD male of breeding from Institute of Experimental Animals, Chinese Academy of Medical Sciences
Rat 70, body weight is 140 160 grams, credit number:SCXK- capital 2009-002.
1.2 experiment material:Low selenium feed:Materials of wheat, corn and soybean etc. are purchased from Sanmenxia City Lingbao City of Henan Province low selenium ground
Area, is processed by experimental animal center of henan province, and through Co60 radiation sterilization, Se content≤0.03ug/g;Respectively by embodiment 1 He
The sample 1 of comparative example 1 preparation and sample 2.
1.3 instruments and reagent
ADVIA1200 automatic clinical chemistry analyzer, KY-2000 semi-automatic biochemical analyzer, DS-671 type electronic balance,
DTS-3 low speed autobalancing centrifuge, DY89-1 type electric driven glass refiner, ASF-930 dual channel atomic fluorescence photometers;Paddy Guang
Sweet peptide peroxidase (GSH-Px) kit (lot number 20090522);Rat thioredoxin reductase (TrxR), ELISA inspection
Test agent box (LOT:08-12).
1.4 experimental technique
70 male SD rats are randomly divided into 7 groups by body weight, raise and Se-low animal is set up with low selenium feed.Weekly from
Every group extract 1 carry out tail vein blood, survey its Blood Se concentration, after 7 weeks when blood selenium mean value be reduced to 0.25mg/L about when, then
It is randomly divided into 7 groups by body weight, 1 group of control group, sample 1 and each 3 groups of sample 2, every group 10, control group daily gavage pure water, sample
Product 1 distinguish the corresponding sample of gavage for 2 groups daily with sample, are respectively according to the dosage of sample daily recommended amounts rat oral gavage
0.4th, 0.8,1.2g/kg (be equivalent to people and recommend 10,20,30 times of consumption) body weight, continuous gavage 1 month, disconnected after last gavage 24h
Head takes blood, centrifugation serum, and wins hepatic and renal tissue, is blotted stand-by with filter paper after ice-cold normal saline flushing.
1.5 index determining
Blood Se content:Hydride atomic fluorescent spectrum method for detecting.Weigh appropriate liver and renal tissue preparation 10%
Tissue homogenate, 3000r/min is centrifuged 15min, takes supernatant, illustrate to measure respectively glutathione peroxidase by kit
(GSH-Px) vigor content.The mensure biuret method of albumen in homogenate.
1.6 statistical analysis data adoptsRepresent, the comparison of mean value is checked with the t of two independent samples.
2. result
2.1 rat blood selenium levels
The impact to Blood Se concentration for table 9 sample
Note:* compare with negative control group, P<0.05.
As seen from the above table, sample 1 and 2 all can increase rat blood Se content, and each dosage group of sample 1 and middle and high dose of sample 2
Amount group is compared with negative control group, and difference has conspicuousness (P<0.05).
2.2 rat livers and renal glutathione peroxidase (GSH-PX) vigour
The impact to liver GSH-PX vigor for table 10 sample
Note:* compare with negative control group, P<0.05;* is compared with negative control group, P<0.01.
As seen from the above table, sample 1 and each dosage group of sample 2 are compared with negative control group, liver glutathione peroxidase
Enzyme activity increases, and each dosage group of sample 1 and sample 2 high dose group vigor increase difference and have conspicuousness P<0.05, particularly sample
Difference highly significant (the P of each dosage group of product 1<0.01).
The impact to kidney GSH-PX vigor for table 11 sample
Note:* compare with negative control group, P<0.05;* is compared with negative control group, P<0.01.
From table 11, sample 1 and each dosage group of sample 2 are compared with negative control group, and kidney GSH-PX vigor all has increasing
Plus, but, the low, high dose group of sample 2 and control group comparing difference be not notable, and the middle and high dosage group of sample 1 and control group ratio
Relatively, difference highly significant (P<0.01).
In sum, rationally, each component reaches preferable effect, L- glutamy to the Halth-care composition formula of the present invention
To the absorption of selenium and utilization, blood Se content substantially increases the reasonably combined body that dramatically increases of amine, and promotes blood selenium in each internal organs
Make full use of, the increase of liver kidney GSH-PX vigor highly significant, and final excellent effect is shown to mouse immunity lifting
Really.
The invention is not limited in aforesaid specific embodiment.The present invention expands to and any discloses in this manual
New feature or any new combination, and the arbitrary new method of disclosure or the step of process or any new combination.
Claims (7)
1. a kind of Halth-care composition of strengthen immunity, said composition includes Ganodenna Lucidum P.E 100-150 weight portion, bat moth
Paecilomyces varioti bacterium powder 100-150 weight portion, sodium selenite 0.01-0.1 weight portion are it is characterised in that described composition also includes L-
Glutamine 50-150 weight portion.
2. the Halth-care composition of strengthen immunity as claimed in claim 1 is it is characterised in that described composition includes glossy ganoderma
Extract 120-150 weight portion, peacilomyce hepiahi bacterium powder 100-130 weight portion, sodium selenite 0.01-0.05 weight portion, L-
Glutamine 80-120 weight portion.
3. the Halth-care composition of strengthen immunity as claimed in claim 1 is it is characterised in that described composition includes glossy ganoderma
Extract 130 weight portion, peacilomyce hepiahi bacterium powder 120 weight portion, sodium selenite 0.03 weight portion, Glu 100 weight
Amount part.
4. the Halth-care composition of strengthen immunity as claimed in claim 1 is it is characterised in that described composition includes glossy ganoderma
Extract 120 weight portion, peacilomyce hepiahi bacterium powder 130 weight portion, sodium selenite 0.05 weight portion, Glu 120 weight
Amount part.
5. the preparation of the Halth-care composition of strengthen immunity described in a kind of any one of 1-4 containing claim, its feature exists
In described preparation is tablet, pill, capsule, powder or granule.
6. the preparation of strengthen immunity as claimed in claim 5 is it is characterised in that described preparation is capsule.
7. a kind of preparation method of capsule as claimed in claim 6, comprises the steps:
Get the raw materials ready:Weigh Ganodenna Lucidum P.E, peacilomyce hepiahi bacterium powder, Co-60 irradiation sterilization, 4kGy;Pulverized respectively after irradiation
80 mesh sieves, standby;Sodium selenite, microcrystalline cellulose, starch, silica material pulverized 80 mesh sieves respectively, standby;
Mixing:Sodium selenite is mixed with starch equal increments method, obtains mixed powder, by mixed powder and Ganodenna Lucidum P.E,
Mixing in mixer put in the lump by the materials such as peacilomyce hepiahi bacterium powder, microcrystalline cellulose, silica and magnesium stearate
40min, makes material mix, and obtains total mixed powder;
Capsule is filled:By mixed material, automatic capsule filling machine is filled with, operating condition:18~26 DEG C, relatively wet
Degree:45%~60%.
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