CN106138289A - Health care oral liquid of sleep and preparation method thereof is improved for enhancing immunity - Google Patents

Health care oral liquid of sleep and preparation method thereof is improved for enhancing immunity Download PDF

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CN106138289A
CN106138289A CN201610651559.XA CN201610651559A CN106138289A CN 106138289 A CN106138289 A CN 106138289A CN 201610651559 A CN201610651559 A CN 201610651559A CN 106138289 A CN106138289 A CN 106138289A
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sleep
parts
enhancing immunity
oral liquid
health care
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李璐
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FUJIAN RUNXING BIOLOGICAL TECHNOLOGY Co Ltd
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FUJIAN RUNXING BIOLOGICAL TECHNOLOGY Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/062—Ascomycota
    • A61K36/066—Clavicipitaceae
    • A61K36/068—Cordyceps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
    • A61K31/198—Alpha-amino acids, e.g. alanine or edetic acid [EDTA]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/076—Poria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
    • A61K36/725—Ziziphus, e.g. jujube
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
    • A61K9/0056—Mouth soluble or dispersible forms; Suckable, eatable, chewable coherent forms; Forms rapidly disintegrating in the mouth; Lozenges; Lollipops; Bite capsules; Baked products; Baits or other oral forms for animals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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    • A—HUMAN NECESSITIES
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction

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Abstract

The present invention relates to a kind of health care oral liquid improving sleep for enhancing immunity, it is mainly formulated according to following weight portion by following components: 45 55 parts of Paecilomyces hepiali Chen et Dai Mycelia powder, Semen Ziziphi Spinosae 45 55 parts, 45 55 parts of Poria, arginine 9 12 parts, oligomeric isomaltose 45 55 parts and Purified Water q. s.The method have the advantages that its not only good absorbing effect, availability height, and sleep can be improved by enhancing immunity.Product the experiment proved that, steady quality, edible safety, and enhancing immunity effect is obvious.

Description

Health care oral liquid of sleep and preparation method thereof is improved for enhancing immunity
Technical field
The present invention relates to a kind of medicine medicine field of health care food, be particularly used for enhancing immunity and improve the health care of sleep Oral liquid and preparation method thereof.
Background technology
Immunity refers to that body resists external invasion and attack, safeguards the ability of homoiostasis.Along with the development of society, people Competitive pressure increasing, at heart anxiety, health is overworked, exercise is inadequate etc. causes the inducement of hypoimmunity more to come The most universal, and along with the process of aged tendency of population, in crowd, immunocompromised person gets more and more.
Sleep is the physiological activity that the mankind are most important and most basic, account for life 1/3rd time.Enough with high-quality The sleep of amount is that the mankind are necessary.Along with the modern life, study, the quickening of work rhythm, most people is faced with huge pressure Power, stress long time integration, brain overload operation, be easily caused brain fag, muscle power overdraw, show as further insomnia, The diseases such as depression, anxiety, psychasthenia.Insomnia has become the second largest disease of department of neurology outpatient service after headache.
Immunocompromised person, sleep state gastrointestinal disease patient often links together with " subhealth state " state." subhealth state " state is Refer to some the functional changes without organic disease, the transient state between health and disease.
One research of World Health Organization (WHO) shows: sleep disorder is one in the world and is the most fully paid attention to and well The public health problem solved, the people in the whole world about 27% suffers sleep disturbances.The material that Chinese Medical Association provides shows, China is about There are 300,000,000 adults to suffer from sleep disorder, are mainly distributed on the area that China's economic is relatively flourishing, but the problem of this respect is always Do not cause enough attention.Sleep disorder association of Britain investigation display: the traffic accident of the U.S. annual 45% and the industrial injury of 55% Accident all causes due to sleeping disorders.Whole world people's every day about 3000 dies from sleeping disorders.45-54 year crowd's insomnia rate Beginning to ramp up, 75-92 year is the highest, and women is up to 60%, and male is 30%.CSRS 2003 is to China 5,000,000 Individual family has carried out sleep health survey, and result shows the mistake of the people's various degrees having 38.2% in China urbanite Dormancy symptom, has considerable people to have a sleepless night the most throughout the year.Progressively favored by people for the health food improving sleep research and development.
Summary of the invention
The present invention provides a kind of health care oral liquid improving sleep for enhancing immunity, its not only good absorbing effect, profit Expenditure is high, and can enhancing immunity, improvement sleep.
The present invention is achieved by the following technical solutions:
A kind of for enhancing immunity improve sleep health care oral liquid, it mainly by following components according to following weight portion Formulated: Paecilomyces hepiali Chen et Dai Mycelia powder 45-55 part, Semen Ziziphi Spinosae 45-55 part, Poria 45-55 part, arginine 9-12 part, Oligomeric isomaltose 45-55 part and Purified Water q. s.
A kind of for enhancing immunity improve sleep health care oral liquid, it mainly by following components according to following weight portion Formulated: 50 parts of Paecilomyces hepiali Chen et Dai Mycelia powder, Semen Ziziphi Spinosae 50 parts, 50 parts of Poria, arginine 11.2 parts, oligomeric different Fructus Hordei Germinatus Sugar 50 parts and Purified Water q. s.
A kind of for enhancing immunity improve sleep health care oral liquid, it mainly by following components according to following weight portion Formulated: 45 parts of Paecilomyces hepiali Chen et Dai Mycelia powder, Semen Ziziphi Spinosae 45 parts, 45 parts of Poria, arginine 10 parts, oligomeric isomaltose 45 parts and Purified Water q. s.
A kind of preparation method of the health care oral liquid improving sleep for enhancing immunity, it is characterised in that:
Comprise the following steps:
(1) Semen Ziziphi Spinosae and Indian buead tablet and Paecilomyces hepiali Chen et Dai Mycelia powder after first pulverizing stir, and then put into In frying pan, add input amount more than 8 times purified water, soaks more than 30 minutes, use normal pressure 100 DEG C decoction 1.5-2.5 afterwards Hour, decoct more than 1 time, decoction liquor is moved in material-compound tank；
(2) weigh arginine and oligomeric isomaltose, and they are put in material-compound tank, then supplement purified water to producing Fill requirement, 200-400 rev/min is stirred more than 30 minutes afterwards, makes arginine and oligomeric isomaltose dissolve fully；
(3) then adjusting the pH value of the solution after above-mentioned mixing with the sodium carbonate of 20% food grade is 6.8-7.0.
Further comprising the steps of: the solution kieselguhr after above-mentioned adjustment pH value to be filtered, obtains material liquid, afterwards by raw material Liquid carries out fill, sterilizing, checks and pack warehouse-in.
In above-mentioned steps (1), decoction process needs be stirred continuously, and need to decoct before decoction liquor immigration material-compound tank Liquid carries out board and frame machine 300 mesh and filters.
The present invention use above-mentioned formula according to being:
According to theory of Chinese medical science foundation, in conjunction with modern study achievement, propose above-mentioned to improve sleep for enhancing immunity Health care oral liquid.Because sleep disorder is many, by the change of feelings will, (stone is built and is carried in obstinate asomnia specific clinics diagnosis and treatment scheme Arrive), feelings will is unsuccessful, the liver failing to maintain the normal flow of QI, the most then mechanism of qi pent-up, if with the passing of time, visible stagnated QI transforming into fire, god's uneasiness then can not be slept.The stagnation of QI is then Blood stasis, hematogenous blockage, liver can not store blood, blood failing to tonify the heart, body, without being depended on, is to be insomnia also.Therefore make a general survey of pathogenesis, QI and blood is unbalance nothing but, from Liver is started with, happy gas, makes gas promoting the circulation of blood live, and irritability is adjusted and reached, and the motive connects with brain gas phase, then the state of mind is from peace, and random dream is also put down, and merrily enters Sleep.
And, the present invention, based on Paecilomyces hepiali Chen et Dai Mycelia powder and arginine, with harmonizing the functional activities of vital QI, dispersing the stagnated live-QI to relieve the stagnation of QI, rises Clear the turbid descending；Be aided with Semen Ziziphi Spinosae, Poria is put down punching and is calmed the nerves, and assistant makes oligomeric isomaltose expel the heat-evil reality；All tastes match, and long memorial irritability, lets out altogether Stagnated fire, determines liver soul, the merit of relieving palpitation god.Cordyceps is the famousst and precious tonic Chinese medicine, and described in " Bencao Congxin ", it has tonifying the lung benefit Effect of kidney, the Paecilomyces hepiali Chen et Dai Mycelia powder of the present invention is fermented cordyceps hypha body, the fermented cordyceps hypha of artificial culture The composition of body, purposes are similar to natural Cordyceps.Modern experimental research shows that (Mu Xiaoqun bites huge at evaluation health food The experimental technique of cytophagy impact is mentioned), Cordyceps mycelium is improved the phagocytic activity of pulmonary macrophage, mice phagocytosis The ability of cleaning up of cell, promotion lymphocyte transformation, the serum hemolysin improving mice and cell immunity of spleen hemolytic activity, have The effect of the strongest enhancing human body immunity power.Showing through numerous studies, Cordyceps mycelium is with natural Cordyceps composition extremely Similar, and pharmacological action is the most similar with clinical effectiveness, is better than natural cordyceps (Mu Xiaoqun and Wei Tao the most in some aspects Evaluating experimental technique and the Cordyceps mycelium improvement immunity merit that macrophage phagocytic function is affected by health food respectively Mention in the research of energy).The result of study prompting Cordyceps myceliums such as Wei Tao have raising mouse systemic nonspecific immunity Function.
Semen Ziziphi Spinosae is the dry mature seed of Rhamnaceae plant Ziziphi Spinosae.It is that calming soporific more typically is important, and " legendary god of farming is originally Grass warp " in it is classified as top grade, Compendium of Material Medica is classified as this category, " Chinese materia medica " teaching material is classified as tranquilizing by nourishing the heart and wants class.It There is tonifying liver, mind calming, arresting sweating, the function promoted the production of body fluid, be used for the diseases such as restlessness of asrhenia type and insomnia, palpitation with fear dreaminess, Tianjin wound be thirsty.Semen Ziziphi Spinosae contains There are saponins, triterpenes, flavonoid, alkaloids, fatty oils, protein, phosphide compounds etc..The pharmacology card of Semen Ziziphi Spinosae Bright unsaturated fatty acids acid moieties has obvious tranquilizer syngignoscism, can substantially shorten the sleep latency that pentobarbital sodium causes Phase, extend pentobarbital sodium length of one's sleep of causing, and to extend its effect the most obvious along with administration time, does not occur tolerating Phenomenon.
Poria is the dry sclerotia of On Polyporaceae Poria.The traditional Chinese medical science thinks that Poria has promoting diuresis to eliminate damp pathogen, spleen invigorating mind calming merit Energy；It is clinically used for treating the diseases such as edema oliguria, phlegm retention vertigo and palpitation, insufficiency of the spleen lack of appetite, malaise, palpitation with fear insomnia.Modern pharmacology grinds Studying carefully and show, Poria and carboxymethyl pachyman have the raising effect such as immunity of organisms, antibacterial, blood sugar lowering.Experimental result all shows, Glutamic acid is arrived again Cytosolic Free Calcium Concentration in Brain Nerve Cells rising by Poria inhibitory action；The neurocyte line grain that folded ammonia sodium is caused The function of body and micro-tubular structure damage protected effect；Suppression to guinea pig skin TYR enzyme mRNA gene expression dose, rises The content of high senile rat hydroxyproline, display has prevention and treatment central nervous system disease, delay skin aging effect. Pachyman inside and outside is administered, and can induce Human Lymphocytes prosperity IFN-γ,IFN-α, IL-2, IL-6, TNF and GM-CSF, energy Being remarkably reinforced mice and tumor-bearing mice Spleen cell proliferation effect, strengthen the phagocytic function of peritoneal macrophage, antagonism CY immunity presses down Make and use.Result shows that pachyman is a kind of immunostimulant.
Arginine is a kind of semi-dispensable amino acid, for normal person, belongs to non essential amino acid, but at some pathology Under state, then it it is essential amino acids.Its metabolite is polyamines, nucleic acid, nitric oxide and nitrogen oxide etc., and the above two are cell The necessary material of division, then both are vasodilation, hepatocyte synthetic protein and liver cell mitochondria electron transmission Important medium, has the effect promoting growth with maintaining organism balance.It addition, arginine belongs to required under metabolic stress state Aminoacid, can be produced impact to immune system, mainly be acted on by cellular immunization generation, to humoral immunity of organism then without notable shadow Ring.Arginine basic role in body is as follows: strengthen during arginic gastrointestinal nutrition supports, no matter through enteral feeding or Through intravenous applications all can stimulating growth hormone, prolactin antagonist, insulin, the release of glucagon, arginine can be in enhancing body Nitrogen retention, effectively play regulation effect, control protein renewal, promote intramuscular albumen synthesis, contribute to improve Body nitrogen balance, improves the immune state of body.Arginic immune defence and immunomodulating: it is thin that arginine can increase T lymph Born of the same parents' reactivity to Con-A and PHA, increases the quantity of cd4 cell, and can improve the NK cell number in peripheral blood and IL-2 is thin The activity of born of the same parents, promotes the generation of IL-2, increases function and the expression of induction IL-2R of cytotoxic T lymphocyte.Application is containing ammonia The enteral nutrition of base acid even can also improve the immunologic function of HIV/AIDS patient, and the major function of intestinal mucosa is to absorb enteric cavity Interior various nutrient substance, are the most also the barriers stoping enteric cavity antibacterial generation dystopy, and the immunologic function of body are to determine carefully The key factor of bacterium whether transposition.
Oligomeric isomaltose is one of China's exploitation functional oligose the earliest, is with refined corn starch for raw material warp A kind of new type functional starch sugar that enzymatic hydrolysis refines.The sugariness of oligomeric isomaltose is the 40% of sucrose, is low sweet Degree, low in calories.It has the promotion superpower propagation of bacillus bifidus, dental caries, allaying tiredness, the absorption of promotion calcium, enhancing body The physiological function such as immunity, loosening bowel to relieve constipation.Because of it, there is low sugariness, low heat value, low viscosity, moisture retention, non-fermented, acidproof again The characteristics such as property, so being widely used in all kinds of health food, audio frequency, milk product, confection and wheaten food etc..
Paecilomyces hepiali Chen et Dai Mycelia powder contains protein, polysaccharide, adenosine, mannitol, free ammonia in aminoacid and 24 in 16 Base acid；
Poria contain diterpene carboxylic acid, polysaccharide, histidine, adenine, choline, β-pachyman enzyme, protease, fatty acid, Fat soft phospholipid, ergosterol, su-fuling etc.；
Containing saponins, triterpenes, flavonoid, alkaloids, fatty oils, protein, fats chemical combination in Semen Ziziphi Spinosae Thing etc..
The method have the advantages that
It not only good absorbing effect, availability high, and sleep can be improved by enhancing immunity.
Product the experiment proved that, steady quality, edible safety, and enhancing immunity effect is obvious.
Detailed description of the invention
Below in conjunction with detailed description of the invention and specific embodiment, the present invention is expanded on further.
The Paecilomyces hepiali Chen et Dai Mycelia powder preferred Zhejiang pharmaceutical Co. Ltd of Wan Feng enterprise group of the present invention, it meets can Specify for health food fungus strain.Health food listed in by Semen Ziziphi Spinosae, Poria, and to declare No. 51 files of raw materials used list attached Within part 1.Arginine is common aminoacid, and oligomeric isomaltose is low-calorie sweeting agent, during above-mentioned each raw material all meets China's people's republic's pharmacopeia and respective quality standard.
(1) detailed description of the invention
A kind of for enhancing immunity improve sleep health care oral liquid, it mainly by following components according to following weight portion Formulated: Paecilomyces hepiali Chen et Dai Mycelia powder 45-55 part, Semen Ziziphi Spinosae 45-55 part, Poria 45-55 part, arginine 9-12 part, Oligomeric isomaltose 45-55 part and Purified Water q. s.
A kind of for enhancing immunity improve sleep health care oral liquid, it mainly by following components according to following weight portion Formulated: 50 parts of Paecilomyces hepiali Chen et Dai Mycelia powder, Semen Ziziphi Spinosae 50 parts, 50 parts of Poria, arginine 11.2 parts, oligomeric different Fructus Hordei Germinatus Sugar 50 parts and Purified Water q. s.
A kind of for enhancing immunity improve sleep health care oral liquid, it mainly by following components according to following weight portion Formulated: 45 parts of Paecilomyces hepiali Chen et Dai Mycelia powder, Semen Ziziphi Spinosae 45 parts, 45 parts of Poria, arginine 10 parts, oligomeric isomaltose 45 parts and Purified Water q. s.
A kind of preparation method of the health care oral liquid improving sleep for enhancing immunity, it is characterised in that:
Comprise the following steps:
(1) Semen Ziziphi Spinosae and Indian buead tablet and Paecilomyces hepiali Chen et Dai Mycelia powder after first pulverizing stir, and then put into In frying pan, add input amount more than 8 times purified water, soaks more than 30 minutes, use normal pressure 100 DEG C decoction 1.5-2.5 afterwards Hour, decoct more than 1 time, decoction liquor is moved in material-compound tank；
(2) weigh arginine and oligomeric isomaltose, and they are put in material-compound tank, then supplement purified water to producing Fill requirement, 200-400 rev/min is stirred more than 30 minutes afterwards, makes arginine and oligomeric isomaltose dissolve fully；
(3) then adjusting the pH value of the solution after above-mentioned mixing with the sodium carbonate of 20% food grade is 6.8-7.0.
Further comprising the steps of: the solution kieselguhr after above-mentioned adjustment pH value to be filtered, obtains material liquid, afterwards by raw material Liquid carries out fill, sterilizing, checks and pack warehouse-in.
In above-mentioned steps (1), decoction process needs be stirred continuously, and need to decoct before decoction liquor immigration material-compound tank Liquid carries out board and frame machine 300 mesh and filters.
Specific embodiment 1
50 parts of Paecilomyces hepiali Chen et Dai Mycelia powder, Semen Ziziphi Spinosae 50 parts, 50 parts of Poria, arginine 11.2 parts, oligomeric different Fructus Hordei Germinatus Sugar 50 parts and Purified Water q. s.
A kind of preparation method of the health care oral liquid improving sleep for enhancing immunity, it is characterised in that:
Comprise the following steps:
(1) Semen Ziziphi Spinosae and Indian buead tablet and Paecilomyces hepiali Chen et Dai Mycelia powder after first pulverizing stir, and then put into In rustless steel jacketed pan, add input amount 8 times purified water, soaks 30 minutes, afterwards with normal pressure 100 DEG C decoction 1.5 hours, Decoct 1 time, then decoction liquor is entered board and frame machine 300 mesh and filters, decoction liquor is moved in material-compound tank；Decoction process needs not Disconnected stirring,
(2) weigh arginine and oligomeric isomaltose, and they are put in material-compound tank, then supplement purified water to producing Fill requirement, 200 revs/min are stirred more than 30 minutes afterwards, make arginine and oligomeric isomaltose dissolve fully；
(3) then adjusting the pH value of the solution after above-mentioned mixing with the sodium carbonate of 20% food grade is 6.8, by above-mentioned adjustment Solution after pH value kieselguhr filters, and obtains material liquid,
(4) after, material liquid is carried out fill, sterilizing, checks and pack warehouse-in.
Specific embodiment 2
45 parts of Paecilomyces hepiali Chen et Dai Mycelia powder, Semen Ziziphi Spinosae 45 parts, 45 parts of Poria, arginine 10 parts, oligomeric isomaltose 45 parts and Purified Water q. s.
A kind of preparation method of the health care oral liquid improving sleep for enhancing immunity, it is characterised in that:
Comprise the following steps:
(1) Semen Ziziphi Spinosae and Indian buead tablet and Paecilomyces hepiali Chen et Dai Mycelia powder after first pulverizing stir, and then put into In rustless steel jacketed pan, add input amount 8 times purified water, soaks 40 minutes, decocts 2 hours with normal pressure 100 DEG C afterwards, pan-fried Boil 1 time, then decoction liquor is entered board and frame machine 300 mesh and filters, decoction liquor is moved in material-compound tank；Decoction process needs constantly Stirring,
(2) weigh arginine and oligomeric isomaltose, and they are put in material-compound tank, then supplement purified water to producing Fill requirement, 300 revs/min are stirred more than 30 minutes afterwards, make arginine and oligomeric isomaltose dissolve fully；
(3) then adjusting the pH value of the solution after above-mentioned mixing with the sodium carbonate of 20% food grade is 7.0, by above-mentioned adjustment Solution after pH value kieselguhr filters, and obtains material liquid,
(4) after, material liquid is carried out fill, sterilizing, checks and pack warehouse-in.
Specific embodiment 3
55 parts of Paecilomyces hepiali Chen et Dai Mycelia powder, Semen Ziziphi Spinosae 55 parts, 55 parts of Poria, arginine 12 parts, oligomeric isomaltose 55 parts and Purified Water q. s.
A kind of preparation method of the health care oral liquid improving sleep for enhancing immunity, it is characterised in that:
Comprise the following steps:
(1) Semen Ziziphi Spinosae and Indian buead tablet and Paecilomyces hepiali Chen et Dai Mycelia powder after first pulverizing stir, and then put into In rustless steel jacketed pan, add input amount 8 times purified water, soaks 40 minutes, decocts 2 hours with normal pressure 100 DEG C afterwards, pan-fried Boil 1 time, then decoction liquor is entered board and frame machine 300 mesh and filters, decoction liquor is moved in material-compound tank；Decoction process needs constantly Stirring,
(2) weigh arginine and oligomeric isomaltose, and they are put in material-compound tank, then supplement purified water to producing Fill requirement, 300 revs/min are stirred more than 30 minutes afterwards, make arginine and oligomeric isomaltose dissolve fully；
(3) then adjusting the pH value of the solution after above-mentioned mixing with the sodium carbonate of 20% food grade is 7.0, by above-mentioned adjustment Solution after pH value kieselguhr filters, and obtains material liquid,
(4) after, material liquid is carried out fill, sterilizing, checks and pack warehouse-in.
Being detected by the product of above example, result shows, the functional component of this product is crude polysaccharides, every 100 millis Rise in finished product containing crude polysaccharides 0.8 gram.
Following principle is used to measure the crude polysaccharides content in product: the polymer substance of food middle-molecular-weihydroxyethyl ＞ 10000 exists In 800ml/L ethanol solution precipitate, separate with water solublity monosaccharide and oligosaccharide, with alkalescence cupric reagent selectivity from other In polymer substance, precipitation has the polysaccharide of glucose structure, reacts with phenolsulfuric acid with carbohydrate versions colorimetric determination Its content, its color intensity is directly proportional to the content of crude polysaccharides glucosan, calculates crude polysaccharides content in food with this.
The following is the experiment to this product enhancing immunity:
The product of this above-described embodiment is carried out zoopery, selects the 18-of Military Medical Science Institute's animal center breeding 22 grams of Kunming kind health cleaning grade female mices 192, are divided into 4 batches, and every batch is randomly divided into 4 groups, and often group 12, wherein tests A collection of carry out internal organs/weight ratio pH-value determination pH, delayed allergy experiment, half hemolysis value (HC50) mensuration and antibody tormation The mensuration of cell, tests two batches and carries out carbonic clearance experiment；Test three batches and carry out Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell in fact Test, test four batches and carry out ConA inducing mouse lymphocyte transformation experiment and NK cytoactive experiments experiment animal feeding in Beijing Practical writing college of science of associated university function of health food inspection center SPF level animal housing, laboratory animal uses credit number: SYXK (capital) 2002-0021.
Dosage: the recommended dose of this product oral liquid is adult's (by 60 kg body weight), every day 90 milliliters, is equivalent to 1.5 milligrams/day/kilogram BW, experiment sets the 5 of human body recommended amounts, 10,30 times respectively, i.e. milligram/day every day 7.5/kilogram BW, 15.0 milligrams/day/kilogram BW, 45.0 milligrams/day/kilogram BW be basic, normal, high dosage group by given the test agent 3750 milliliters, through 60- 70 DEG C are evaporated to 1520 milliliters, add sterilized water 1667 milliliters, make 2.25 times of concentrated solutions of given the test agent as high dose Indices is surveyed after tested material.Mouse stomach volume is 0.2 milliliter/10g Mus weight, sets a blank group (0 milligram/thousand simultaneously Gram BW), replace tested material with water (sterilizing), every day, gavage volume was identical with each tested material group.
Key instrument and reagent: T500 electronic balance (20000006), AE100 electronic balance (96009), 755 light splitting light Degree meter (2002001), microplate reader (98001), CO2 gas incubator (96090), low speed centrifuge (98090), water bath with thermostatic control (2004009), microscope (2003002), inverted microscope (98006), spiral micrometer (96099).
Clean bench, sterile surgical instrument, microsyringe (25 microlitre), cell counter, 24 holes and 96 holes are flat Tissue Culture Plate, the 96 U-shaped Tissue Culture Plates in hole, glass culture plate, glass dish, gauze, test tube, slide frame, 200 eye mesh screens, Timer, color device suction pipe, microscope slide.
Mianyang erythrocyte (SRB), normal saline, Hank ' s liquid (PH7.2-7.4) RPM11640 culture fluid, calf serum, Penicillin, streptomycin, concanavalin A, Con A (ConA-), 1% glacial acetic acid, the hydrochloric acid solution of 1mol/L, acid isopropyl alcohol (96mL isopropyl Alcohol adds the hydrochloric acid solution 4mL of 1mol/L), MTT PBS (PH7.2-7.4), complement (guinea pig serum), SA buffer, fine jade Lipolysaccharide, Dou Shi reagent (sodium bicarbonate 1.0g, high-potassium ferricyanide PH7.2-7.4), potassium cyanide 0.05g, add distilled water to 1000 millis Rise), YAC-1 cell, EINECS 212-761-8, nitro tetrazolium chloride, PMS, oxidized form of nicotinamide-adenine dinucleotide, 0.2mol/L Tris-HCl solution (PH8.2), 1%NP40, india ink, 0.1% sodium bicarbonate, chicken red blood cell, methanol, Giemsa dye liquor Deng.
Experimental technique
The mensuration of organ weight ratio value
After mouse weights, cervical dislocation is put to death, and takes spleen and thymus, removes most fascia, blots organ surface blood stains with filter paper and claim Weight, calculates spleen weight ratio and thymus body weight ratio.
Delayed allergy (DTH) experiment (the foot sole of the foot thickens method)
Taking Sanguis caprae seu ovis, brine 3 times, every Mus is through lumbar injection 2% (v/v, with normal saline) hematocrit SRBC (2000r/min, 10min) 0.2mL, 4d after sensitization, measure left footpad portion thickness, and same position is measured three times, makes even Average.Then subcutaneous registration 20% (v/v, with normal saline) the hematocrit SRBC20 microlitre in measuring point, in injection after 24 Hour measure left footpad portion thickness, represent the degree of DTH with the difference of foot sole of the foot thickness before and after attacking.The difference of given the test agent group Value is significantly higher than the difference of matched group, can determine that this experimental result positive.
The mouse lymphocyte transformation experiment (mtt assay) of ConA induction
Aseptic take spleen, be placed in the little plate filling appropriate aseptic Hank ' s liquid, gently spleen ground with tweezers, make list Individual cell suspension.Filter through 200 eye mesh screens, make cell suspension.Wash 3 times with Hank ' s, the most centrifugal 10 minutes.Then will be thin Born of the same parents are suspended in the complete culture solution of 1 milliliter, and microscopy counts, and investigation cell concentration is 3x106Individual/milliliter, then splenocyte is hanged Liquid point holes adds in 24 well culture plates, 1 milliliter of every hole, and a hole adds 75 microlitre ConA liquid and (is equivalent to 7.5 micrograms/in the least wherein Rise), 5% carbon dioxide, as comparison, is put in another hole, cultivates 72 hours in 37 DEG C of carbon dioxide incubators.Culture fluid, is simultaneously introduced MTT (5 mg/ml) 50 microlitres/hole, continues to cultivate 4 hours.After cultivation terminates, every hole adds colorimetric on 1 milliliter of photometer and surveys Fixed, wavelength 570nm.The multiplication capacity of lymphocyte cuts off the optical density value generation being not added with ConA hole by the optical density value adding ConA hole The multiplication capacity of table lymphocyte.The optical density difference of given the test agent group is significantly higher than the optical density difference of matched group, can determine that This experimental result positive.
The mensuration of antibody-producting cell
Taking Sanguis caprae seu ovis, brine 3 times, every Mus is through lumbar injection 2% (v/v, with normal saline) hematocrit SRBC (2000r/min, 10min) 0.2mL, by SRBC immunity 5 days after mice cervical dislocation put to death, take out spleen, clear spark-out Broken spleen, makes cell suspension.Centrifugal (1000r/min) 10min, washes 2 times with Hank ' s liquid, is finally existed by cell suspension In 8mLHank ' s liquid.After agar heating for dissolving, Hank ' s leaf double with equivalent mixes, and subpackage small test tube does not has pipe 0.5 milliliter, Add 10% in pipe again (v/v, with SA liquid prepare) hematocrit SRBC 50 microlitre, splenocyte suspension 8 microlitre, rapidly after mixing, topples over In the microscope slide of brush agarose thin layer, do parallel plate, after agar solidification, slide level is buckled and is placed on horse, add two 37 DEG C of incubations 1 hour in carbonoxide incubator, then join in slide frame groove with SA buffer dilution complement (1:8), continue After continuous incubation 1.5h, counting hemolysis plaque number, represent with plaque number/full splenocyte, the plaque number of given the test agent group is significantly high In the plaque number of matched group, can determine that this experimental result positive.
Half hemolysis value (HC50) mensuration
Taking Sanguis caprae seu ovis, brine 3 times, every Mus is through lumbar injection 2% (v/v, with normal saline) hematocrit SRBC carries out immunity, after 5 days, extracts eyeball and takes blood in centrifuge tube, place about 1h, peeled off with tube wall by solidification blood, make serum Fully separating out, 2000r/min is centrifuged 10min, collects serum.It is 300 times with SA buffer by serum-dilution, takes 1 milliliter and put examination In pipe, being sequentially added into 10% (v/v prepares) hematocrit SRBC 0.5 milliliter with SA liquid, complement 1 milliliter (presses 1:8 with SA buffer dilute Release).Separately setting the control tube (replacing with SA buffer) of not increase serum and be placed in 37 DEG C of waters bath with thermostatic control constant temperature after 15 minutes, ice bath is eventually Only reaction.2000r/ minute is centrifugal 10 minutes, takes supernatant 1 milliliter, adds Dou Shi reagent to 4mL in another test tube, fully mixes, After placing 10 minutes, take 540 nanometers and make blank with control tube, measure each pipe optical density value respectively.The amount of hemolysin is with half Haemolysis value (HC50 represents), is calculated as follows:
Optical density value x extension rate during sample half hemolysis value=sample optical density value/SRBC HD50；
The HC50 of given the test agent group is significantly higher than the HC50 of matched group, can determine that this experimental result positive.
Clearance in mice is tested
Enclose intravenous injection by body weight from mice and dilute the india ink (0.05mL/10g) of 4 times.Treat that prepared Chinese ink injects, count immediately Time, inject after prepared Chinese ink 2,10 minutes, take blood 20 microlitre from the interior venous plexus that adjoins respectively, and it is molten to be added into 2 milliliter of 0.1% sodium carbonate In liquid, at 600nm wavelength, measure optical density value (OD) with 755 spectrophotometers, make blank with sodium carbonate liquor, by little Mus is put to death, and takes liver and spleen is weighed.Represent that the ability of carbonic clearance is calculated as follows a with carbonic clearance index (a):
K=(lgOD1-lgOD2)/(t2-t1) a=body weight=(liver weight+spleen weight) * k1/3
The carbonic clearance index of given the test agent group is significantly higher than the carbonic clearance index of matched group, can determine that this experimental result sun Property.
Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell experiment (half intracorporal method)
Mouse peritoneal injection 20% (v/v, with normal saline) chicken red blood cell (2000r/min, 10min).Suspend 1mL, is spaced 30min, and cervical dislocation is put to death, and is faced upward position and is fixed on Mus plate, through abdominal cavity saline injection 2Ml, rotates Mus Plate 1min.Take peritoneal macrophage washing liquid 1Ml.Take peritoneal macrophage washing liquid 1mL, drip respectively on 2 microscope slides, put into pad In having the enamel box of wet gauze, move to incubate incubator in 37 DEG C and educate 30min, incubate complete, rinse in normal saline, to remove non-paster Cell.Drying, fix with 1:1 acetone methanol solution, 4% (v/v) Giemsa-phosphate buffer dyes, then rinses with distilled water Dry, count under oil mirror, 100 macrophages of every counting, it is calculated as follows phagocytic rate and phagocytic index:
Macrophage number × 100 of the macrophage number/counting of percentage phagocytosis (%)=phagocytosis chicken red blood cell
The phagocyte of the chicken red blood cell sum/counting of phagocytic index=phagocytosis
The phagocytic percentage drawn carries out data conversion the most as the following formula,In formula, p is phagocytic percentage, With phagocytic percentage and the phagocytic index of matched group, can determine that this experimental result positive.
The mensuration (lactate dehydrogenase L DH algoscopy) of NK cytoactive
Testing first 24 hours and target cell YAC-1 carries out Secondary Culture, application Hank ' s liquid is washed 3 times, with containing 10% calf It is 4 × 10 that the RPMI1640 complete culture solution of serum adjusts cell concentration5Individual/mL.Test mice cervical dislocation is put to death, and aseptic takes Spleen, makes splenocyte suspension, washes 2 times with application Hank ' s liquid, the most centrifugal 10 minutes (1000r/min).Abandoning supernatant will be thin Endochylema is upspring, and adds 0.5mL aquesterilisa 20 seconds, adds 0.5mL2 times of Hank ' s liquid and 8mLHank ' s after splitting erythrocyte, 1000r/min, is centrifuged for 10 minutes, and resuspended with the 1mL RPMI1640 complete culture solution containing 10% calf serum, microscopy counts, and uses It is 2 × 10 that RPMI1640 complete culture solution adjusts cell concentration7Individual/mL, makes effect target than for 50:1, takes target cell and effector lymphocyte Each 100 microlitres, add in U-shaped 96 well culture plate, and target cell Spontaneous release hole adds target cell and each 100 microlitres of culture fluid, and target is thin Born of the same parents' maximum release aperture adds each 100 microlitres of 1%NP40, above-mentioned every is all provided with three parallel holes, 37 DEG C, 5% CO2 gas incubator 96 orifice plates are centrifuged 5 minutes by middle cultivation 4 hours with 1500r/min, and 96 well culture plates at the bottom of supernatant 100 microlitre horizontalization are drawn in every hole In, add LDH matrix liquid 100 microlitre, react 3-10 minute, then every hole addition 1mol/LD HCl solution 30 microliter termination is anti- Should, densitometric value (OD) at microplate reader 490nm, calculating NK cytoactive:
NK cytoactive (%)=(reacting hole OD-Spontaneous release hole OD)/(maximum release aperture OD-Spontaneous release hole OD) × 100
The NK cytoactive drawn carries out data conversion as the following formula,In formula, P is NK cytoactive, with little Number represents.Obtaining data is measurement data, and the NK cytoactive of given the test agent group is significantly higher than the NK cytoactive of matched group, can Judge this experimental result positive.
Data process
Data process is carried out with SPSS software.Use variance analysis, but it is neat to need the program of variance analysis first to carry out variance Property inspection, variance is neat, calculates F value, F value ＜ F0.05, conclusion: no significant difference between each group mean；F value >=F0.05, P≤0.05, Add up with the comparative approach two-by-two of mean between multiple experimental grouies and a matched group, to abnormal or the number of variance polishing According to carrying out the conversion of suitable variable, after meeting normal state or variance requires together, add up by the data after conversion, if variable Still it is not up to normal state or the neat purpose of variance after conversion, uses rank test instead and add up.
Result judgment basis
" health food inspection with assessment technique specification " (2003 editions) regulation: cellular immune function, humoral immune function, Any two aspect results of monocytes/macrophages function, four aspects of NK cytoactive are positive, can determine that this given the test agent has increasing Strong immunity function effect.Wherein two experimental results in cellular immune function assay project are the positive, or any one is real Two the dosage group results tested are positive, can determine that humoral immune function measurement result is positive.Monocytes/macrophages functional examination item Two experimental results in mesh are the positive, or two dosage group results of any one experiment are positive, can determine that monokaryon-huge is bitten thin Born of the same parents more can the result positive.More than one dosage group result of NK cytoactive detection experiment is positive, can determine that NK cytoactive is tied Fruit is positive.
Result
The product of the present invention impact on Mouse Weight
The original body mass of each group mice
From table 1, the original body mass of mice compares with between 0ML/kgBW group four batches of laboratory animal each dosage groups, difference There are no significant, and (P ＞ 0.05), the i.e. original body mass of mice more equalize at each assembly.
The impact on Mouse Weight of table 2 product of the present invention
From table two, per os gave the product oral liquid of the present invention of mice various dose after 32 days, and the body weight of mice exists Four batches of laboratory animal each dosage groups compared with 0ML/kgBW assembly, there are no significant for difference (P ＞ 0.05), product the most of the present invention Mouse Weight is had no adverse effects.
The product of the present invention impact on mice organs/body weight ratio
The impact on mouse spleen/body weight ratio of table 3 product of the present invention
From table 3, per os gave the product oral liquid of the present invention of mice various dose after 32 days, the spleen/body of mice Focus on four batches of laboratory animal each dosage groups compared with 0ML/kgBW assembly, there are no significant for difference (P ＞ 0.05), the i.e. present invention Mouse spleen/body weight is affected by product without special.
The impact on mouse thymus/body weight ratio of table 3 product of the present invention
From table 4, per os gave the product oral liquid of the present invention of mice various dose after 32 days, the thymus/body of mice Focus on four batches of laboratory animal each dosage groups compared with 0ML/kgBW assembly, there are no significant for difference (P ＞ 0.05), the i.e. present invention Mouse thymus/body weight is affected by product without special.
The product of the present invention impact on mouse cell immunologic function
The impact on mice delayed allergy (DTH) of table 5 product of the present invention
From table 5, per os gave the product oral liquid of the present invention of mice various dose after 32 days, the pedal swelling of mice Spend four batches of laboratory animal each dosage groups compared with 0ML/kgBW assembly, there are no significant for difference (P ＞ 0.05), the i.e. present invention Product on mice delayed allergy ability without impact.
The impact on mouse spleen lymphocyte transformation experiment of table 6 product of the present invention
※: compared with 0ML/kgBW group, there is significant difference
From table 6, per os gave the product oral liquid of the present invention of mice various dose after 32 days, 15.0ML/kgBW, Compared with between 45.0ML/kgBW with 0ML/kgBW group, the competence for added value of lymphocyte all has significant difference (P ＜ 0.05), i.e. Product of the present invention can strengthen ConA inducing mouse Splenic vein hemodynamics ability at 15.0ML/kgBW, 45.0ML/kgBW.
The product of the present invention impact on humoral immunization
The impact on mouse antibodies cellulation number of table 7 product of the present invention
※: compared with 0ML/kgBW group, there is significant difference
From table 7, per os gave the product oral liquid of the present invention of mice various dose after 32 days, 45.0ML/kgBW with Comparing between 0ML/kgBW group, antibody-producting cell number has significant difference (P ＜ 0.05), and product the most of the present invention is at 45.0ML/ KgBW can improve mouse antibodies cellulation number.
Table 8 product of the present invention is to mice half hemolysis value (HC50) impact
※: compared with 0ML/kgBW group, there is significant difference
From table 7, per os gave the product oral liquid of the present invention of mice various dose after 32 days, 15.0ML/kgBW, Compared with between 45.0ML/kgBW with 0ML/kgBW group, half hemolysis value all has significant difference (P ＜ 0.05), the i.e. present invention to produce Product all can improve mice half hemolysis value at 15.0ML/kgBW, 45.0ML/kgBW.
The product of the present invention impact on mouse monokaryon-macrophage phagocytic function
The impact on mice carbonic clearance ability of table 9 product of the present invention
From table 9, per os gave the product oral liquid of the present invention of mice various dose after 32 days, each dosage group mice carbon Ability of cleaning up compares with 0ML/kgBW group, no significant difference (P ＞ 0.05), product the most of the present invention carbonic clearance ability without shadow Ring.
The impact on mouse macrophage phagocytosis chicken red blood cell phagocytic rate of table 10 product of the present invention
※: compared with 0ML/kgBW group, there is significant difference
From table 10, per os gave the product oral liquid of the present invention of mice various dose after 32 days, 45.0ML/kgBW with Comparing between 0ML/kgBW group, phagocytic rate has significant difference (P ＜ 0.05), and product 45.0ML/kgBW the most of the present invention can improve Mouse macrophage phagocytosis chicken red blood cell phagocytic rate.
The impact on mouse macrophage phagocytosis chicken red blood cell phagocytic index of table 11 product of the present invention
※: compared with 0ML/kgBW group, there is significant difference
From table 11, per os gave the product oral liquid of the present invention of mice various dose after 32 days, 45.0ML/kgBW with Comparing between 0ML/kgBW group, phagocytic index has significant difference (P ＜ 0.05), and product 45.0ML/kgBW the most of the present invention can carry High mouse macrophage phagocytosis chicken red blood cell phagocytic index.
The product of the present invention impact on NK cells in mice activity
The impact on NK cells in mice activity of table 12 product of the present invention
From table 12, per os gave the product oral liquid of the present invention of mice various dose after 32 days, each dosage group with Compare between 0ML/kgBW group, there was no significant difference (P ＞ 0.05), product the most of the present invention to the NK cytoactive of mice without shadow Ring.
Brief summary
Per os gave the product oral liquid of the present invention of mice various dose after 32 days, compared with 0ML/kgBW group, and this is tested Thing can strengthen the mouse spleen lymphocyte conversion capability of ConA induction (P is less than 0.05) in 15.0mL/kgBW group, improve mice half Number haemolysis values, 45.0mL/kgBW group can strengthen ConA induction mouse spleen lymphocyte conversion capability, improve mice huge bite thin Endocytosis bites chicken red blood cell phagocytic rate, improves mouse macrophage phagocytosis chicken red blood cell phagocytic index.This tested material is to Mouse Weight Growth has no adverse effects.According to " health food inspection and assessment technique specification " (2003), enhancing immunity health food is sentenced Calibration will definitely be known, product of the present invention has the function of enhancing immunity.
The following is the experiment that product of the present invention is improved sleep
1.1 materials and method
Sample: product of the present invention
1.2 laboratory animals: select the 18-22g health cleaning of Beijing Vital River Experimental Animals Technology Co., Ltd.'s breeding Male mice 180, is divided into 3 batches and tests, and every batch is randomly divided into 4 groups, often group 15.Test a collection of reality of directly sleeping Test and extend the mouse sleep time experiment of pentobarbital sodium induction；Test two batches and carry out dosage hypnosis reality under pentobarbital sodium valves Test, test three batches and carry out pentobarbital sodium Sleep latency experiment.
The recommended dose of the 1.3 dosage present invention for adult (by 60kg weighing machine) 90mL every day, be equivalent to 1.5mL/ day/ kgBW.Experiment sets the 5 of human body recommended amounts, 10,30 times respectively, i.e. every day: 7.5mL/kgBW, 15.0mL/kgBW, 45.0mL/ KgBW by given the test agent 3750 milliliters for basic, normal, high dosage group, is evaporated to 1520 milliliters through 60-70 DEG C, adds sterilized water 1667 milliliters, make 2.25 times of concentrated solutions of given the test agent as surveying indices after high dose tested material.Mouse stomach volume It is 0.2 milliliter/10g Mus weight, sets a blank group (0 mg/kg BW) simultaneously, replace tested material with water (sterilizing), often Day gavage volume is identical with each tested material group.
1.4 instruments and reagent
Instrument: AE100 electronic balance (96009), T500 electronic balance (2000006), stopwatch (2001003)
Reagent pentobarbital sodium, barbital sodium, normal saline
Detection method
Directly sleep experiments
Observe tested treated animal and give the given the test agent of 3 dosage, after matched group gives same volume solvent, if occur sleeping Dormancy phenomenon.Sleep is with righting reflex loss as index.When mice is placed in supine position, body position can be righted immediately.As more than 30-60 Second can not right body, i.e. thinks that righting reflex loss, entrance are slept, and righting reflex recovers to be animal awakening.Righting reflex disappears Losing to recovering is the animal sleep time during this period of time, record blank group and tested material group sleep number of animals and the length of one's sleep.
The length of one's sleep is measurement data, uses variance analysis, and animal of falling asleep is enumeration data, uses x2Inspection, the most right According to group and experimental group sleep animal and the difference between the length of one's sleep, if sleep number of animals or the length of one's sleep increase significance, Then experimental result is positive.
1.5 extend the experiment length of one's sleep
After last gives solvent and variable concentrations tested material, 15min before peak effect occurs, to each treated animal lumbar injection 32mg/kgBWmL/kgBW, injection volume is 0.1mL/kgBW.With mice righting reflex loss for sleep index, observe tested material energy The length of one's sleep of no prolongation pentobarbital sodium induction.Experiment was carried out at night.
The length of one's sleep is measurement data.Difference between comparative experiments group and the matched group prolongation length of one's sleep.The length of one's sleep Extend and have significance, then experimental result is positive.
Dosage hypnosis experiment under pentobarbital sodium valves
Carry out preliminary experiment before formal experiment, determine HD under pentobarbital sodium valves, i.e. 80-90% mice righting reflex After the pentobarbital sodium maximum threshold values dosage last not disappeared gives solvent and variable concentrations tested material, before peak effect occurs 15min, to each treated animal lumbar injection 26mg/kgBWmL/kgBW, injection volume is 0.1mL/kgBW.Disappear with mice righting reflex Can it be sleep standard that mistake reaches more than 1 minute person, sleep animal in recording 30 minutes, observe tested material and extend pentobarbital sodium valves Lower dosage sleep animal incidence rate.Experiment was carried out at night.
Sleep animal is measurement data, uses x2Inspection.Difference between comparative experiments group and matched group sleep animal.Enter Sleep animal incidence rate increase and have significance, then experimental result is positive.
Barbital sodium Sleep latency is tested
After tested material 20min that last gives solvent and difference is taken a sea-voyage eastward, each treated animal lumbar injection 240mL/kgBW barbital Sodium, injection volume is 0.1mL/10kgBW. with animal righting reflex loss for sleep index, observes tested material and sleeps barbital sodium Preclinical impact.
Sleep latency is measurement data.Difference between comparative experiments group and matched group Sleep latency, sleep latency Phase shortening has significance, then experimental result is positive.
1.6 data process
Data process is carried out with SPSS software.Use variance analysis, but it is neat to need the program of variance analysis first to carry out variance Property inspection, variance is neat, calculates F value, F value ＜ F0.05, conclusion: no significant difference between each group mean；F value >=F0.05, P≤0.05, Add up with the comparative approach two-by-two of mean between multiple experimental grouies and a matched group, to abnormal or the number of variance polishing According to carrying out the conversion of suitable variable, after meeting normal state or variance requires together, add up by the data after conversion, if variable Still it is not up to normal state or the neat purpose of variance after conversion, uses rank test instead and add up.
1.7 result judgment basis
Extend dosage hypnosis experiment under the pentobarbital sodium experiment length of one's sleep, pentobarbital sodium valves, barbital sodium sleep is dived It is positive that the volt phase tests two lanes in three experiments, and without the most directly sleep effect, can determine that this given the test agent has improvement sleep Function.
Result
The impact on world's sleep experiments of table 13 product of the present invention
By matched group seen from table 13 and three dosage treated animals in giving tested material 60 minutes, all do not have and directly sleep Dormancy phenomenon.
The product of the present invention impact on the pentobarbital sodium induced hypnotic time
The impact on the pentobarbital sodium inducing mouse length of one's sleep of table 14 product of the present invention
Group Number of animals (only) The length of one's sleep (min) P value
0mL/kgBW 15 22.0±5.2 ——
7.5mL/kgBW 15 25.6±7.7 0.392
15.0mL/kgBW 15 25.6±8.5 0.391
45.0mL/kgBW 15 32.4±7.1※ 0.001
※: compare with 0mL/kgBW group and there is significant difference
From table 14, per os gives this product 30 days of mice various dose, 45.0mL/kgBW group and 0mL/kgBW group Relatively, there is significant difference (P ＜ 0.01) time of sleep, and product the most of the present invention can extend penta bar ratio in 45.0mL/kgBW group Appropriate sodium inducing mouse length of one's sleep.
Product of the present invention is on the impact of dosage hypnosis experiment under pentobarbital sodium valves
Table 15 product of the present invention is on the impact of dosage mice sleep incidence rate under pentobarbital sodium valves
From table 15, per os gives this product 30 days of mice various dose, 45.0mL/kgBW group and 0mL/kgBW group Relatively, sleep animal incidence rate has significant difference (P ＜ 0.05), and product the most of the present invention can improve penta in 45.0mL/kgBW group Dosage mice sleep animal incidence rate under barbital sodium valves.
The product of the present invention impact on barbital sodium Sleep latency
The impact on barbital sodium Sleep latency of table 16 product of the present invention
From table 16, per os gives this product 30 days of mice various dose, 45.0mL/kgBW group and 0mL/kgBW group Relatively, Sleep latency has significant difference (P ＜ 0.05), and product the most of the present invention can shorten mice bar in 45.0mL/kgBW group Than appropriate sodium Sleep latency.
Brief summary
Per os gives this product 30 days of mice various dose, and matched group and three dosage groups are giving tested material 60 minutes In, all for by phenomenon of directly sleeping.Product of the present invention is when 45.0mL/kgBW group can extend the sleep of pentobarbital sodium inducing mouse Between.Product the most of the present invention is dosage mice sleep animal incidence rate under 45.0mL/kgBW group can improve pentobarbital sodium valves.I.e. originally Invention product can shorten mice barbital sodium Sleep latency in 45.0mL/kgBW group.According to " health food inspection and evaluation skill Art specification " (2003 editions) to improve sleep health food criterion understand, product of the present invention have improve sleep function.
Sum up: product of the present invention not only good absorbing effect, availability are high, and can enhancing immunity, improvement sleep.
Below be only illustrating of the possible embodiments for the present invention, but this embodiment and be not used to limit the present invention The scope of the claims, all without departing from thinking of the present invention do equivalence implement or change, be intended to be limited solely by the scope of the claims of the present invention In.

Claims (7)

1. one kind for enhancing immunity improve sleep health care oral liquid, it is characterised in that: it mainly by following components according to Following weight portion is formulated: Paecilomyces hepiali Chen et Dai Mycelia powder 45-55 part, Semen Ziziphi Spinosae 45-55 part, Poria 45-55 part, essence ammonia Acid 9-12 part and oligomeric isomaltose 45-55 part.
The health care oral liquid improving sleep for enhancing immunity the most according to claim 1, it is characterised in that: it is main Formulated according to following weight portion by following components: 50 parts of Paecilomyces hepiali Chen et Dai Mycelia powder, Semen Ziziphi Spinosae 50 parts, Poria 50 Part, arginine 11.2 parts and oligomeric isomaltose 50 parts.
The health care oral liquid improving sleep for enhancing immunity the most according to claim 1, it is characterised in that: it is main Formulated according to following weight portion by following components: 45 parts of Paecilomyces hepiali Chen et Dai Mycelia powder, Semen Ziziphi Spinosae 45 parts, Poria 45 Part, arginine 10 parts and oligomeric isomaltose 45 parts.
4. the preparation method of the health care oral liquid improving sleep for enhancing immunity, it is characterised in that:
Comprise the following steps:
(1) first Semen Ziziphi Spinosae and Poria are pulverized, Semen Ziziphi Spinosae after then pulverizing and Poria and Paecilomyces hepiali Chen et Dai Mycelia powder Stir, then above-mentioned mixed powder is put in frying pan, and put into the purified water of mixed powder quality more than 8 times, immersion More than 30 minutes, decoct 1.5-2.5 hour afterwards, decoct more than 1 time, decoction liquor is moved in material-compound tank；
(2) weigh arginine and oligomeric isomaltose, and they are put in material-compound tank, then supplement purified water to producing fill Requirement, stirs more than 30 minutes with 200-400 rev/min afterwards, makes arginine and oligomeric isomaltose dissolve fully.
The preparation method of a kind of health care oral liquid improving sleep for enhancing immunity the most according to claim 4, its Be characterised by: after above-mentioned steps completes, with sodium carbonate that mass percentage concentration is 20% food grade adjust after above-mentioned mixing molten The pH value of liquid is 6.8-7.0.
The preparation method of a kind of health care oral liquid improving sleep for enhancing immunity the most according to claim 5, its It is characterised by: the solution kieselguhr after above-mentioned adjustment pH value is filtered, obtains material liquid, afterwards material liquid is carried out fill, goes out Bacterium, check and pack warehouse-in.
The preparation method of a kind of health care oral liquid improving sleep for enhancing immunity the most according to claim 4, its It is characterised by: in step (1), decoction process needs be stirred continuously, and need decoction liquor before decoction liquor immigration material-compound tank Filter.
CN201610651559.XA 2016-08-10 2016-08-10 Health care oral liquid of sleep and preparation method thereof is improved for enhancing immunity Pending CN106138289A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107223966A (en) * 2017-05-09 2017-10-03 新疆生命核力高科股份有限公司 It is a kind of to be used to improve sweet oral liquid of Rong's grass of immunity and preparation method thereof
CN107668445A (en) * 2017-10-18 2018-02-09 中国农业科学院特产研究所 A kind of useful for sleeping, the beverage for adjusting immunity and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
养生营: "修元干宝口服液", 《HTTPS://WWW.DOUBAN.COM/NOTE/564334823/》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107223966A (en) * 2017-05-09 2017-10-03 新疆生命核力高科股份有限公司 It is a kind of to be used to improve sweet oral liquid of Rong's grass of immunity and preparation method thereof
CN107223966B (en) * 2017-05-09 2020-11-06 新疆生命核力高科股份有限公司 Roxburgh rose and licorice oral liquid for improving immunity and preparation method thereof
CN107668445A (en) * 2017-10-18 2018-02-09 中国农业科学院特产研究所 A kind of useful for sleeping, the beverage for adjusting immunity and preparation method thereof

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