CN106387351A - Compound Chinese herbal medicinal feed additive and preparation method thereof - Google Patents
Compound Chinese herbal medicinal feed additive and preparation method thereof Download PDFInfo
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- CN106387351A CN106387351A CN201610970988.3A CN201610970988A CN106387351A CN 106387351 A CN106387351 A CN 106387351A CN 201610970988 A CN201610970988 A CN 201610970988A CN 106387351 A CN106387351 A CN 106387351A
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- Prior art keywords
- folium
- herb medicine
- fermentation
- folium ginkgo
- aspergillus niger
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- 238000002360 preparation method Methods 0.000 title claims description 13
- 239000003674 animal food additive Substances 0.000 title abstract description 10
- 150000001875 compounds Chemical class 0.000 title abstract description 4
- 241000218628 Ginkgo Species 0.000 claims abstract description 32
- 235000011201 Ginkgo Nutrition 0.000 claims abstract description 32
- 235000008100 Ginkgo biloba Nutrition 0.000 claims abstract description 32
- 238000000855 fermentation Methods 0.000 claims abstract description 25
- 230000004151 fermentation Effects 0.000 claims abstract description 25
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 22
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 18
- 241001508813 Clavispora lusitaniae Species 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 7
- 239000008103 glucose Substances 0.000 claims abstract description 7
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 7
- 239000000654 additive Substances 0.000 claims description 20
- 230000000996 additive effect Effects 0.000 claims description 20
- 239000002131 composite material Substances 0.000 claims description 19
- 239000003814 drug Substances 0.000 claims description 19
- 239000004459 forage Substances 0.000 claims description 15
- 239000002054 inoculum Substances 0.000 claims description 13
- 244000005700 microbiome Species 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 239000007836 KH2PO4 Substances 0.000 claims description 3
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 241000228212 Aspergillus Species 0.000 claims description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims 1
- 230000000474 nursing effect Effects 0.000 claims 1
- 235000001368 chlorogenic acid Nutrition 0.000 abstract description 35
- 241001465754 Metazoa Species 0.000 abstract description 10
- 235000016709 nutrition Nutrition 0.000 abstract description 8
- 230000035764 nutrition Effects 0.000 abstract description 7
- 230000036039 immunity Effects 0.000 abstract description 5
- 229930003935 flavonoid Natural products 0.000 abstract description 4
- 150000002215 flavonoids Chemical class 0.000 abstract description 4
- 235000017173 flavonoids Nutrition 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 3
- 239000007787 solid Substances 0.000 abstract description 3
- 239000000796 flavoring agent Substances 0.000 abstract description 2
- 235000019634 flavors Nutrition 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 235000019629 palatability Nutrition 0.000 abstract description 2
- 239000013543 active substance Substances 0.000 abstract 1
- 230000001502 supplementing effect Effects 0.000 abstract 1
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 34
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 34
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 34
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 34
- 229940074393 chlorogenic acid Drugs 0.000 description 34
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 34
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 34
- 229930003944 flavone Natural products 0.000 description 26
- 235000011949 flavones Nutrition 0.000 description 26
- 239000000243 solution Substances 0.000 description 23
- 150000002213 flavones Chemical class 0.000 description 21
- 230000012010 growth Effects 0.000 description 12
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 7
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 7
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 7
- 235000005493 rutin Nutrition 0.000 description 7
- 229960004555 rutoside Drugs 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 5
- 235000013330 chicken meat Nutrition 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 210000000582 semen Anatomy 0.000 description 5
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 5
- 241000287828 Gallus gallus Species 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 150000002212 flavone derivatives Chemical class 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000013558 reference substance Substances 0.000 description 4
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 210000003746 feather Anatomy 0.000 description 3
- 230000031700 light absorption Effects 0.000 description 3
- 206010013786 Dry skin Diseases 0.000 description 2
- 239000000899 Gutta-Percha Substances 0.000 description 2
- 240000000342 Palaquium gutta Species 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- -1 flavone compound Chemical class 0.000 description 2
- 229920000588 gutta-percha Polymers 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000005375 photometry Methods 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 235000013594 poultry meat Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 235000010288 sodium nitrite Nutrition 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- BDMCAOBQLHJGBE-UHFFFAOYSA-N C60-polyprenol Natural products CC(=CCCC(=CCCC(=CCCC(=CCCC(=C/CCC(=C/CCC(=C/CCC(=C/CCC(=C/CCC(=C/CCC(=C/CCC(=C/CO)C)C)C)C)C)C)C)C)C)C)C)C BDMCAOBQLHJGBE-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000305071 Enterobacterales Species 0.000 description 1
- 241000208689 Eucommia ulmoides Species 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 229930186185 Polyprenol Natural products 0.000 description 1
- 229920001731 Polyprenol Polymers 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 235000019730 animal feed additive Nutrition 0.000 description 1
- 230000002180 anti-stress Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 210000001669 bursa of fabricius Anatomy 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 229940111260 ginkgo folium Drugs 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
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- 238000005259 measurement Methods 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
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- 244000000010 microbial pathogen Species 0.000 description 1
- 150000003096 polyprenols Chemical class 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention relates to a compound Chinese herbal medicinal feed additive. The compound Chinese herbal medicinal feed additive is mainly produced by a fermentation of the following raw materials in parts by weight by using clavispora lusitaniae and aspergillus niger: 50-60% of folium ginkgo and folium cortex eucommiae, 30-35% of water, 5-12 % of wheat bran, 1-2% of glucose, 1-2% of nitrogen source, and 1-2% of buffer and inorganic salt. The weight ratio of the folium ginkgo to folium cortex eucommiae is (0.5-4):1. A medium is prepared by combining the raw materials of the folium ginkgo and folium cortex eucommiae, the clavispora lusitaniae and aspergillus niger are combined to conduct the solid fermentation, and the feed additive is prepared. The main nutrients and contents of active substances of total flavonoids and chlorogenic acids are greatly increased, and the feed additive is added into feed, so that the flavor and palatability are greatly improved. While supplementing the nutrition of the feed, the feed additive effectively enhances the animal body immunity, increases food safety, improves consumption quality, and enhances taste.
Description
Technical field
The invention belongs to field of animal feed is and in particular to a kind of composite herb medicine forage additive and preparation method thereof.
Background technology
Meat product quality requirements are improved constantly, the hormone of prophyiaxis and promoting growth by modern people great attention safe to food
And antibiotic etc. is also easy to produce the feed additive of Drug resistance and drug residue and is progressively restricted and is even eliminated by pottery.Ensureing feedstuff
On the basis of additive meets national regulation, natural Chinese herbal feed additive has gradually embodied the advantage of oneself.Semen Ginkgo
It is traditional Chinese medicine with the Cortex Eucommiae, they are also rich in flavone compound, terpene lactoness, green in addition to having the nutrition of general leaveves
Ortho acid, polyprenol etc. have the compound of notable biological activity to people and poultry.These active matter mass-energy effectively strengthen animal
The immunologic function of body, promotes the growth of Immune Organs of Body；S. pullonum, Salmonella choleraesuls, big can be suppressed
The multiple pathogenic microorganisms such as enterobacteria；And there is the effect such as the fertility improving animal and growth performance.Research in recent years
Indicate using Folium Ginkgo, Folium Eucommiae and its extract as poultry, domestic animal, aquatic products feed additive, can not only prevent to move
The generation of thing disease, adjusts Immune Function In Animals, reduces prophylactic, increases edible safety moreover it is possible to improve edible quality, increases
Strong mouthfeel.
Content of the invention
The present invention is directed to the problem that existing animal feed additive exists, and provides a kind of chlorogenic acid, flavonoid isoreactivity to become
Divide content high fermentation of Chinese herbal medicine additive, while supplement feed nutrition, strengthen animal body immunity, increase food peace
Quan Xing, improves edible quality.
A kind of composite herb medicine forage additive, mainly by following composition by weight raw material through Clavisporalusitaniae and black fermented preparation
Mould fermentation forms：Folium Ginkgo and Folium Eucommiae 50-60%, water 30-35%, wheat bran 5-12%, glucose 1-2%, nitrogen source 1-2%,
Buffer agent and inorganic salt 1-2%；Described Folium Ginkgo：Folium Eucommiae weight ratio=0.5-4:1.
Further, the inoculum concentration of Clavisporalusitaniae accounts for Folium Ginkgo for Clavisporalusitaniae dry powder and Folium Eucommiae is total
The 0.25-2% of dry weight, the inoculum concentration of aspergillus niger accounts for Folium Ginkgo and the 0.5-4% of Folium Eucommiae gross dry weight for Aspergillus niger spores.
With the addition of appropriate glucose and nitrogen source in above-mentioned component, Clavisporalusitaniae can be started and accelerate into logarithm
Trophophase, Clavisporalusitaniae can produce the nutritional labelings such as carbon source and nitrogen source in growth course.Aspergillus niger utilizes Portugal
The nutrition that excellent spore yeast growth produces and wheat bran provide more labile cellulose nutrition to start Fast-propagation, Jin Erfen
The materials such as the cellulose in solution Folium Ginkgo and Folium Eucommiae, hemicellulose.Clavisporalusitaniae and aspergillus niger symplastic growth, generation
Thank, the composition such as the cellulose that can decompose, converts in Chinese medicine, saccharide, protein, make Chinese medicine cell wall breaking, make chlorogenic acid, flavone
Class material isoreactivity substance release out, and during some metabolites of producing during growth metabolism of microorganism can change
The active component of medicine, improves the feature of product, lifts the property digested and assimilated of leaf simultaneously.
Further, described nitrogen source includes (NH4)2SO4、NH4NO3Deng.
Further, described buffer agent includes KH2PO4、K2HPO4Deng.
Further, described inorganic salt includes MgSO4、MgCl2Deng.
Further, described fermentation temperature is 23-32 DEG C, more preferably 28 DEG C.
Further, described fermentation time is 48-120h, more preferably 96h.
Further, the addition of described additive nutrition purposes, especially for feeding animals is 0.5-4g/100g normal feedstuff, more preferably 1g/
100g.
The preparation method of above-mentioned composite herb medicine forage additive, comprises the following steps：
(1) preparation of fermentation raw material：By the Folium Ginkgo of 50-60% and Folium Eucommiae, 5-12% wheat bran, 1-2% glucose, 1-
2% nitrogen source, 1-2% buffer agent and inorganic salt and excess water mix in proportion, sterilizing；
(2) with Folium Ginkgo and Folium Eucommiae overall dry weight inoculation Clavisporalusitaniae dry powder 0.25-2% and Aspergillus niger spores
0.5-4%, is fermented.
Further, sterilize at sterilising conditions are 121 DEG C 20-30min.
The present invention is with Folium Ginkgo and Folium Eucommiae for raw material compounded culture medium, and joint Clavisporalusitaniae, aspergillus niger are multiple
Close bacterium solid fermentation, the feed additive preparing, its Major Nutrient composition, the active matter based on total flavones and chlorogenic acid
Matter content greatly improves, and makes local flavor and palatability have larger improvement after being added in feedstuff.While supplement feed nutrition,
Effectively strengthen animal body immunity, increase foodsafety, improve edible quality, strengthen mouthfeel.
Brief description
Fig. 1 embodiment 1 Semen Ginkgo and the impact to total flavones and chlorogenic acid content for the Folium Eucommiae proportioning；
The impact to total flavones and chlorogenic acid content for Fig. 2 embodiment 2 aspergillus niger inoculum concentration；
The impact to total flavones and chlorogenic acid content for Fig. 3 embodiment 3 cultivation temperature；
The impact to total flavones and chlorogenic acid content for Fig. 4 embodiment 4 incubation time.
Biological material specimens preservation information：
Clavisporalusitaniae (Clavispora lusitaniae), is preserved in Chinese microorganism strain preservation management committee
Member's meeting common micro-organisms center (CGMCC), address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date：2014
October 20, deposit number is CGMCC No.9836；
Aspergillus niger (Aspergillus.niger), is preserved in China Committee for Culture Collection of Microorganisms's commonly micro- life
Thing center (CGMCC), address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date：On October 20th, 2014, preservation
Numbering is CGMCC No.9776.
Specific embodiment
With reference to specific embodiment and accompanying drawing, the present invention is described in further details.
Material prepares：
Folium Ginkgo：In October, 2014 is collected in Nanjing Forestry University Semen Ginkgo garden, drying leaf；Folium Eucommiae：In December, 2014 adopts
Combine in Institutes Of Technology Of He'nan's Cortex Eucommiae garden, leaf-fall is collected, after natural drying；Wheat bran：Commercially available.The equal 60 DEG C of bakings of above test material
Dry, grinds, cross 40 mesh sieves, and sealing preserve is standby.
Total flavones, the extraction of chlorogenic acid：Weigh dry composite herb fermenting thing 5.0g, in ultrasonic power 500W condition
Under, solid-to-liquid ratio 1:15th, under the conditions of concentration of alcohol 70%, Extracting temperature are 50 DEG C, extract 20min, twice, merging carries for accumulative extraction
Take liquid.
The mensure of general flavone content：(1) control substance of Rutin solution preparation：Precision weighs the reed in 120 DEG C of dryings to constant weight
Fourth reference substance 20mg, puts in 100ml volumetric flask, plus 70% appropriate amount of ethanol, and putting slight fever on water-bath (40 DEG C) makes dissolving, lets cool,
Plus 70% ethanol to scale, shake up, obtain final product the control substance of Rutin solution of 0.2mg/ml.(2) visible photometry scanner uni standard is bent
The preparation of line：Pipette control substance of Rutin solution 0.0ml, 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml, 6.0ml respectively to put
In the volumetric flask of 25ml, respectively add water to 6.0ml, plus 5% sodium nitrite solution 1.0ml, mix, place 6min, plus 10% nitric acid
Aluminum solutions 1.00ml, shakes up, and places 6min, plus 4% sodium hydroxide solution 10ml, adds water to scale, shakes up, and places 15min.
With 0.0ml rutin configuration solution as blank, carry out spectral scan in the range of 200～800nm, obtain at 505nm as visible ray
Maximum absorption band.With blank reagent as reference, by 6 standard solutions mensuration absorbance under 505nm respectively, with rutin comparison
The concentration of product is abscissa, draws standard curve with its absorbance for vertical coordinate, obtains regression equation A1=11.1556C1+
0.0061, R=0.9997.Rutin concentration is good in 0.0～48.0mg/L scope linear relationship.(3) extracting solution is centrifuged, sucking filtration
Afterwards, whole constant volumes, in 200ml volumetric flask, accurately measure respectively in the volumetric flask of 2.5ml, 5ml to 25ml, respectively add water to
6.0ml, plus 5% sodium nitrite solution 1.0ml, mix, and place 6min, plus 10% aluminum nitrate solution 1.00ml, shake up, and place
6min, plus 4% sodium hydroxide solution 10ml, add water to scale, shake up, and place 15min.Measure its light absorption value at 505nm,
According to total flavones concentration C in its standard curve regression equation calculation extracting solution1(mg/L).
The mensure of chlorogenic acid content：(1) preparation of chlorogenic acid reference substance solution：Precision weighs in 120 DEG C of dryings to constant weight
Chlorogenic acid reference substance 20mg, put in 100ml volumetric flask, bonus point analysis straight alcohol is appropriate, put heating (60 DEG C) on water-bath make molten
Solution, lets cool, and bonus point analysis straight alcohol, to scale, shakes up, obtains final product the chlorogenic acid reference substance solution of 0.2mg/ml.(2) visible photometry
The drafting of scanner uni standard curve：Pipette the above-mentioned comparison of 0.0ml, 0.8ml, 1.6ml, 2.4ml, 3.2ml, 4ml, 4.8ml respectively
Product solution, in the volumetric flask of 25ml, is diluted to scale with 0.2mol/L hydrochloric acid solution, mixes, and this is standard serial solution.With
0.0ml rutin configuration solution is blank, carries out spectral scan, obtain at 328nm for visible ray in the range of 200～800nm
Big absworption peak.Measure light absorption value at 328nm, with the concentration of chlorogenic acid standard substance as abscissa, with its absorbance as vertical coordinate
Draw standard curve, obtain regression equation：C2=19.0490A1- 0.0744, R=0.9984.Chlorogenic acid concentration 0.0～
38.4mg/L scope linear relationship is good.(3) extracting solution is centrifuged, after sucking filtration, whole constant volumes in 200ml volumetric flask, respectively
Accurately measure in the volumetric flask of 2.5ml, 5ml to 25ml, be diluted to scale with 0.2mol/L hydrochloric acid solution, mix, at 328nm
Measure its light absorption value, according to its standard curve regression equation calculation extracting solution Content of Chlorogenic Acid concentration C2(mg/L).
Embodiment 1-15 all prepares composite herb medicine forage additive according to following steps：
(1) preparation of fermentation raw material：Folium Ginkgo to 54% and Folium Eucommiae (Folium Ginkgo：Folium Eucommiae weight ratio=0.5-4:
1) 31.5% water, 10% wheat bran, 1.5% glucose, 1% (NH are added in4)2SO4, 1% KH2PO4With 1%
MgSO4·7H2O, mix homogeneously, sterilize at 121 DEG C 20-30min；
(2) in gnotobasiss inoculation 1% Clavisporalusitaniae dry powder and 0.5-4% Aspergillus niger spores (with Semen Ginkgo
Leaf and Folium Eucommiae overall dry weight)；
(3) cultivate 48-120h in 23-32 DEG C of incubator.
Embodiment 1 Folium Ginkgo and the impact to total flavones and chlorogenic acid content for the Folium Eucommiae proportioning
In the case that other conditions (aspergillus niger inoculum concentration 2%, 28 DEG C of fermentation temperature, fermentation time 96h) determine, investigate
Folium Ginkgo and Folium Eucommiae mass ratio (0.5:1、1:1、2:1、3:1、4:1) impact to total flavones and chlorogenic acid content, result
As Fig. 1, with the increase of Folium Ginkgo quality proportion, the more apparent increase of general flavone content, chlorogenic acid content decreases, in ratio
2:1 beginning, chlorogenic acid content reduces substantially.Analysis is probably it is suppressed that mushroom in the presence of Eucommia ulmoides leaf glue larger proportion
Growth.
The impact to total flavones and chlorogenic acid content for the embodiment 2 aspergillus niger inoculum concentration
In other conditions (Folium Ginkgo and Folium Eucommiae mass ratio 2:1,28 DEG C of fermentation temperature, fermentation time 96h) determine
In the case of, investigate the impact to total flavones and chlorogenic acid content for the aspergillus niger inoculum concentration (0.5%, 1%, 2%, 3%, 4%), result
As Fig. 2, with the increase of aspergillus niger inoculum concentration, the content of total flavones and chlorogenic acid all occurs being obviously improved trend, works as inoculum concentration
When reaching 2%, the total flavones amount deceleration of growth, the impact to chlorogenic acid is less.
The impact to total flavones and chlorogenic acid content for embodiment 3 cultivation temperature
In other conditions (Folium Ginkgo and Folium Eucommiae mass ratio 2:1, aspergillus niger inoculum concentration 2%, fermentation time 96h) determine
In the case of, investigate cultivation temperature (23 DEG C, 25 DEG C, 28 DEG C, 30 DEG C, 32 DEG C) to the impact to total flavones and chlorogenic acid content,
Result such as Fig. 3, with the rising of cultivation temperature, the falling after rising of total flavones and chlorogenic acid.
The impact to total flavones and chlorogenic acid content for embodiment 4 incubation time
In other conditions (Folium Ginkgo and Folium Eucommiae mass ratio 2:1, aspergillus niger inoculum concentration 2%, 28 DEG C of fermentation temperature) really
In the case of fixed, investigate the impact to total flavones and chlorogenic acid content for the incubation time (48h, 72h, 84h, 96h, 120h), result
As Fig. 4, with the prolongation of incubation time, the content of total flavones and chlorogenic acid is all obviously improved, and then occurs different degrees of
Decline.
Embodiment 5-15
Fermented product is prepared using different factor parameter combinations, as shown in table 1, each embodiment is adopted the parameter being related to
Parameter and total flavones, chlorogenic acid measurement result as shown in table 2.
Table 1 Semen Ginkgo-Folium Eucommiae composite Chinese herbal fermentation technology factor
Table 2 Semen Ginkgoes-Folium Eucommiae composite Chinese herbal fermentation technology and result
From Table 2, it can be seen that the solid fermentation products general flavone content that embodiment 10 obtains is 2.124%, chlorogenic acid
Content be 0.421%, the optimum process condition of equal highest, therefore fermentation is：Folium Ginkgo：Folium Eucommiae 2：1, aspergillus niger inoculum concentration
2%, 28 DEG C of cultivation temperature, incubation time 96h.
From the point of view of above-described embodiment result：When Folium Eucommiae ratio is higher, the lag phase of microorganism slightly longer it may be possible to due to
The reason contain gutta-percha in Folium Eucommiae, thus speculate that may work as gutta-percha content is reduced to when certain level to microorganism
The impact of growth can be ignored.When microorganism ratio exceedes that a certain amount of or incubation time grows to certain time, total flavones and green
Determination of Chlorogenic Acid does not rise anti-fall it should be due to part can consume Flavonoid substances and chlorogenic acid in microorganism growth process, and
The different growth phases of microorganism consume Flavonoid substances and the ratio of chlorogenic acid also changes.
The product of embodiment 10 is added in animal feed, with raise 7 in advance white feather chicken for feeding trial object, point right
According to group and test group, the input amount of test group is four gradients 0.5%, 1.0%, 1.5%, 2% (g/g normal feedstuff weight),
After feeding 45, by measuring body weight, spleen, thymus, fabricius bursa quality and small intestinal length etc., to calculate bait conversion ratio, point
Analysis various dose composite Chinese herbal tunning to white feather chicken growth promotion, lifting the aspect such as immunity and anti-stress ability effect
Fruit and suitable injected volume.
From the point of view of the result of table 3, experimental group is than the bait conversion ratio of matched group, end weight, thymus index and French capsule index
It is significantly improved respectively, illustrates the central immune organ of white feather chicken is created and significantly affect, improve the immunity of chicken.With
When, in terms of color and luster and myoplasm, chicken meat quality also makes moderate progress.
Table 3 is with respect to matched group, the various index lifting ratios of different additive amount
Claims (10)
1. a kind of composite herb medicine forage additive it is characterised in that main by following composition by weight raw material through Clavispora lusitaniae
Yeast and fermentation of Aspergillus niger form：Folium Ginkgo and Folium Eucommiae 50-60%, water 30-35%, wheat bran 5-12%, glucose 1-2%,
Nitrogen source 1-2%, buffer agent and inorganic salt 1-2%；Described Folium Ginkgo：Folium Eucommiae weight ratio=0.5-4:1；Described Clavispora lusitaniae
Yeast has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC
No.9836；Described aspergillus niger has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number
For CGMCC No.9776.
2. composite herb medicine forage additive according to claim 1 is it is characterised in that described Clavisporalusitaniae
Inoculum concentration accounts for Folium Ginkgo and the 0.25-2% of Folium Eucommiae gross dry weight for Clavisporalusitaniae dry powder, and the inoculum concentration of aspergillus niger is black
Aspergillus spore accounts for Folium Ginkgo and the 0.5-4% of Folium Eucommiae gross dry weight.
3. composite herb medicine forage additive according to claim 1 and 2 is it is characterised in that described fermentation temperature is 23-
32℃.
4. composite herb medicine forage additive according to claim 1 and 2 is it is characterised in that described fermentation time is 48-
120h.
5. composite herb medicine forage additive according to claim 1 and 2 is it is characterised in that described nitrogen source includes (NH4)2SO4、NH4NO3.
6. composite herb medicine forage additive according to claim 1 and 2 is it is characterised in that described buffer agent includes
KH2PO4、K2HPO4.
7. composite herb medicine forage additive according to claim 1 and 2 is it is characterised in that described inorganic salt includes
MgSO4、MgCl2.
8. composite herb medicine forage additive according to claim 1 and 2 is it is characterised in that the nursing of described additive is dynamic
The addition of thing is 0.5-2g/100g normal feedstuff.
9. a kind of preparation method of composite herb medicine forage additive is it is characterised in that comprise the following steps：
(1) preparation of fermentation raw material：By the Folium Ginkgo of 50-60% and Folium Eucommiae, 5-12% wheat bran, 1-2% glucose, 1-2%
Nitrogen source, 1-2% buffer agent and inorganic salt and excess water mix in proportion, sterilizing；
(2) Clavisporalusitaniae dry powder 0.25-2% and Aspergillus niger spores 0.5- is inoculated with Folium Ginkgo and Folium Eucommiae overall dry weight
4%, fermented.
10. the preparation method of composite herb medicine forage additive according to claim 9 is it is characterised in that described sterilizing
Condition is the 20-30min that sterilizes at 121 DEG C, and fermentation condition is 23-32 DEG C of bottom fermentation 48-120h.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110024910A (en) * | 2019-05-17 | 2019-07-19 | 河南农业大学 | The method for improving wormwood tunning quality with cellulase and composite probiotics ferment |
| CN110692848A (en) * | 2018-11-08 | 2020-01-17 | 荆楚理工学院 | Chicken feed containing selenium astaxanthin and DHA thereof |
| CN116162557A (en) * | 2023-02-03 | 2023-05-26 | 大连三仪动物药品有限公司 | A strain of Saccharomyces cerevisiae, chinese medicinal microecological preparation for preventing and treating ruminant diarrhea, and its preparation method |
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| CN1919044A (en) * | 2006-09-20 | 2007-02-28 | 南京林业大学 | Animal feedstuff additive and preparation method thereof |
| CN101543259A (en) * | 2009-05-08 | 2009-09-30 | 南京林业大学 | Preparation method of biological feed additive |
| CN102318743A (en) * | 2011-09-30 | 2012-01-18 | 南京林业大学 | Method for improving ginkgo leaf feeding palatability and increasing product bioactivity |
| CN103355545A (en) * | 2013-07-23 | 2013-10-23 | 南京林业大学 | Fermentation type botanical weaned piglet feed additive |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1919044A (en) * | 2006-09-20 | 2007-02-28 | 南京林业大学 | Animal feedstuff additive and preparation method thereof |
| CN101543259A (en) * | 2009-05-08 | 2009-09-30 | 南京林业大学 | Preparation method of biological feed additive |
| CN102318743A (en) * | 2011-09-30 | 2012-01-18 | 南京林业大学 | Method for improving ginkgo leaf feeding palatability and increasing product bioactivity |
| CN103355545A (en) * | 2013-07-23 | 2013-10-23 | 南京林业大学 | Fermentation type botanical weaned piglet feed additive |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110692848A (en) * | 2018-11-08 | 2020-01-17 | 荆楚理工学院 | Chicken feed containing selenium astaxanthin and DHA thereof |
| CN110024910A (en) * | 2019-05-17 | 2019-07-19 | 河南农业大学 | The method for improving wormwood tunning quality with cellulase and composite probiotics ferment |
| CN116162557A (en) * | 2023-02-03 | 2023-05-26 | 大连三仪动物药品有限公司 | A strain of Saccharomyces cerevisiae, chinese medicinal microecological preparation for preventing and treating ruminant diarrhea, and its preparation method |
| CN116162557B (en) * | 2023-02-03 | 2023-09-01 | 大连三仪动物药品有限公司 | A strain of Saccharomyces cerevisiae, chinese medicinal microecological preparation for preventing and treating ruminant diarrhea, and its preparation method |
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