CN105031220A - Fermented traditional Chinese medicine preparation for preventing broiler sudden death syndrome - Google Patents
Fermented traditional Chinese medicine preparation for preventing broiler sudden death syndrome Download PDFInfo
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- CN105031220A CN105031220A CN201510535095.1A CN201510535095A CN105031220A CN 105031220 A CN105031220 A CN 105031220A CN 201510535095 A CN201510535095 A CN 201510535095A CN 105031220 A CN105031220 A CN 105031220A
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Abstract
The invention discloses a fermented traditional Chinese medicine preparation for preventing broiler sudden death syndrome. A preparation method of the fermented traditional Chinese medicine preparation comprises the following steps: (1) crushing traditional Chinese medicines until the granularity is 60-80 meshes, weighing according to proportions, and mixing in a three-dimensional way to obtain traditional Chinese medicine powder; (2) respectively putting the mixed traditional Chinese medicine powder and 300-600 parts of water into an extracting tank, and soaking for 5-20 hours; (3) heating to 40-100 DEG C and extracting for 10-15 hours; (4) filtering and collecting the liquid medicine; (5) further adding the residual dregs into 300-600 parts of water, and extracting at 40-100 DEG C for 10-15 hours; (6) filtering for removing the dregs, mixing two extracting solutions, and carrying out vacuum concentration at 30-70 DEG C until the volume of the mixed solution is concentrated to 40-50% of the original volume. A traditional Chinese medicine fermentation preparation method comprises the following steps: (1) adding 4% of glucose, 4% of yeast extract powder and 2% of soy peptone into a traditional Chinese medicine concentrated solution, heating to 121 DEG C and sterilizing for 30 minutes; (2) pouring seed liquid, and fermenting for 24 hours at 30-37 DEG C; (3) filling.
Description
Technical field
The present invention relates to a kind of fermented tcm preparation preventing broiler sudden death syndrome.
Background technology
Fermented tcm be by natural drug (Chinese medicine) extracting solution with in preferred beneficial bacteria of intestinal tract flora one or more, one strain or a few probiotics are as strain, utilize microecology, bionic method, by the mode of biological grafting, simulate intestinal environment and the digest and decompose process of Chinese medicine ingredients in human body of human body in vitro, biological conversion is carried out to the Effective Component of Chinese Medicine extracted, by the macromolecular substances of Chinese medicine, the micromolecule composition that directly can be absorbed by human body intestinal canal is become through microbial transformation, Chinese medicine is made to become quick absorption, the newtype drug of quantitative curative effect.Fermented tcm, it is simulation Chinese medicine digest and decompose process in animal body, adopt modern biotechnology, set up outside animal body one " industrialization gastrointestinal system ", adopt the multiple probiotic bacteria in animal intestinal, carry out predigestion, decomposition and conversion to Chinese medicine, macromolecular intermediate material, decomposition and inversion becomes can by the effective small-molecule substance directly absorbed.Therefore, fast, the definite effect of fermented tcm curative effect.Through Long-term clinical and facts have proved, fermented tcm is compared Chinese medicine drug effect and is improved 4-28 doubly.Fermented tcm is through predigestion and conversion.Thorough releasing medicine detrimental toxicities, and eliminate the defect of Chinese medicine decoction " bitter to the taste ", taste takes a turn for the better.Fermented tcm is the great innovation to Chinese medicine, and really achieve Chinese Herbs speed-raising, attenuation synergistic, improving mouthfeel, is the revolution to Chinese medicine industry, starts the epoch of Chinese medicine.It utilizes modern biotechnology to make Chinese medicine and probiotics perfect adaptation, forms emerging Chinese medicine preparation, at antibiotics resistance, under the situation that superbacteria constantly occurs, significant.
Sudden death disease of meat chicken, also known as disease of dying of a sudden illness, acute death syndrome, has been a kind of common disease in broiler production at present.Be mainly in the young chicken that growth is fast.The clinical manifestation sudden death disease of meat chicken course of disease is short, and premorbid is without any different shape; Fast mainly with growth, physically well develop, the young chicken sudden death of fleshiness is feature; Part sudden death chicken premorbid is quieter than normal chicken performance; Feedstuff feed intake reduces, and indivedual chicken is usually when poultry raiser enters to give up feeding, out of control suddenly, and wing is sharply agitated or the liftoff 15 ~ 20cm that takeoffs, and is about lmin from morbidity to the death time; Dead chicken is generally lies with legs pointing up in lying on the back or abdomen prone position gesture, cervical region distortion, muscle spasm, finds that there is unexpected shriek during indivedual chicken morbidity.Cut open inspection change outward appearance build more plentiful, abnormal without other except cockscomb, wattle slightly flushing.Fill the feedstuff of just having searched for food, atrial dilatation in crop and muscular stomach, heart compared with normal chicken is large, and cardiac muscle is soft, and liver enlargement, matter are crisp, color is pale, pulmonary congestion, and chest muscle, abdominal muscle are moistening pale, and the accidental intestinal wall of the dead chicken of minority has bleeding.3. this disease of etiological analysis is how relevant with feeding and management factor (nutrition, illumination, epidemic prevention, raising, density, stress etc.).Broiler is due to fast growth, and some systemic-functions of self (as cardiovascular system, respiratory system, digestive system) grow imperfection; In addition, also too high with protein in feedstuff, fat content, vitamin is unreasonable relevant with mineral proportioning.Broiler due to feed intake large, excess nutrition is taken the photograph in human body and is caused overnutrition, accelerated breathing, heart burden, and sudden death phenomenon occurs.
Sudden death disease of meat chicken is extensively present in aquaculture, does not have good solution yet so far.Provided by the inventionly utilize important fermentation technique to solve this difficult problem there is wide market prospect.
Summary of the invention
Technical problem to be solved by this invention is: provide a kind of fermented tcm preparation preventing broiler sudden death syndrome.
The present invention solves this technical problem adopted technical scheme: in Chinese medicine extraction liquid, add a certain amount of culture medium, access compound probiotic seed liquor.Ferment at a certain temperature.The wherein mixed bacteria liquid that is made up of bacillus megaterium, aspergillus niger, bakery yeast of probiotic bacteria seed liquor, wherein the percentage by weight of all kinds of bacterium is: bacillus megaterium 10%-20%, aspergillus niger 30%-60%, yeast 30%-60%.The preparation method of compound probiotic seed liquor comprises the steps: activated spawn, level liquid amplification culture, secondary liquid amplification culture.Bacillus megaterium, aspergillus niger, bakery yeast secondary amplification culture gained seed liquor are mixed according to the following ratio: bacillus megaterium 10%-20%, aspergillus niger 30%-60%, yeast 30%-60%.Chinese medicine extraction liquid is prepared from by Ramulus Cinnamomi, Radix Glycyrrhizae, Radix Rehmanniae, Radix Ophiopogonis, Fructus Jujubae.Preparation process is: Chinese medicine powder is broken to 60-80 order by (1), takes by formula ratio, and three-dimensional hybrid obtains Chinese medicine powder; (2) mixed Chinese medicine powder and 300-600 part water are put in extraction pot respectively, soak 5-20 hour; (3) be heated to 40-100 DEG C and extract 10-15 hour; (4) collecting by filtration medicinal liquid; (5) remaining medicinal residues continue to add 300-600 part water, and 40-100 DEG C is extracted 10-15 hour; (6) excessively filter medicinal residues, merge extracted twice liquid, 30-70 DEG C is evaporated to original volume 40%-50%.Herb fermenting preparation method is as follows: (1) adds the glucose of 4% in the concentrated liquid of tradition Chinese medicine, and 4% yeast leaching powder, 2% soy peptone, heat up 121 DEG C of sterilizings 30 minutes.(2) with 10-20% inoculum concentration access seed liquor, 30-37 DEG C of bottom fermentation 24 hours.(3) fill.
Beneficial effect of the present invention:
1. adopt the method for herb fermenting, the Chinese medicine macromole not easily absorbed is converted into the micromolecule being easy to absorb, improves drug effect.
2. fermented tcm is through predigestion and conversion.Thorough releasing medicine detrimental toxicities, and eliminate the defect of Chinese medicine decoction " bitter to the taste ", taste takes a turn for the better, and is easy to animal and takes.
3. significantly can reduce the incidence probability of sudden death disease of meat chicken.
Detailed description of the invention
EXAMPLE l
1, the preparation of compound probiotic seed liquor:
1) activation each slant strains of preserving, respectively shake-flask culture, bacillus megaterium uses LB culture medium to cultivate, pH7.3, and 120 DEG C of autoclavings 30 minutes, cultivate 15 hours at 37 DEG C.Aspergillus niger and bakery yeast use PDA culture medium, pH7.5, and 120 DEG C of autoclavings 30 minutes, cultivate 20 hours at 30 DEG C;
2) the level liquid amplification culture (50L tank) of bacillus megaterium, aspergillus niger, bakery yeast; Inoculum concentration 15%;
3) the secondary liquid amplification culture (500L tank) of bacillus megaterium, aspergillus niger, bakery yeast; Inoculum concentration 15%;
4) bacillus megaterium, aspergillus niger, bakery yeast seed liquor are mixed according to the following ratio: bacillus megaterium 20%, aspergillus niger 50%, yeast 30%%;
2, Chinese medicine extraction liquid and preparation method thereof
1) Chinese medicine powder is broken to 80 orders, takes by formula ratio, three-dimensional hybrid obtains Chinese medicine powder;
2) mixed Chinese medicine powder, 500 parts of water are put in extraction pot respectively, soak 8 hours; (3) be heated to 100 DEG C extract 12 hours;
3) collecting by filtration extracting solution;
4) secondary adds 500 parts of water in medicinal residues, and 100 DEG C are extracted 12 hours;
5) merge extracted twice liquid, 60 DEG C are evaporated to original volume 40%;
3, the preparation method of Chinese medicine fermented liquid
1) in the concentrated liquid of tradition Chinese medicine, add the glucose of 4%, 4% yeast leaching powder, 2% soy peptone, heat up 121 DEG C of sterilizings 30 minutes.
2) with 10% inoculum concentration access seed liquor, 30-37 DEG C of bottom fermentation 24 hours.
3) fill.
Claims (6)
1. one kind is prevented the fermented tcm preparation of broiler sudden death syndrome.Said preparation mainly Chinese medicine extraction liquid is made through composite probiotics ferment.
2. the wherein mixed bacterial that is made up of bacillus megaterium, aspergillus niger, bread microzyme of probiotic bacteria seed liquor.In seed liquor, all kinds of bacterium liquid mass percent is: bacillus megaterium 10%-20%, aspergillus niger 30%-60%, yeast 30%-60%.
3. Chinese medicine extraction liquid is prepared from by Ramulus Cinnamomi, Radix Glycyrrhizae, Radix Rehmanniae, Radix Ophiopogonis, Fructus Jujubae.Its mass fraction is as follows: Ramulus Cinnamomi 20-40 part, Radix Glycyrrhizae 20-40 part, Radix Rehmanniae 20-40 part, Radix Ophiopogonis 20-40 part, Fructus Jujubae 20-40 part.
4. the preparation method of probiotic bacteria seed liquor, comprises the steps: to activate each slant strains of preserving, respectively shake-flask culture, bacillus megaterium LB culture medium, pH7-7.5,100-130 DEG C of autoclaving 20-40 minute, cultivates 12-20 hour at 30-38 DEG C.Aspergillus niger, bakery yeast use PDA culture medium, pH7-8,100-130 DEG C of autoclaving 20-40 minute, cultivate 15-25 hour at 30-37 DEG C; The level liquid amplification culture (50L tank) of bacillus megaterium, aspergillus niger, bakery yeast; Inoculum concentration 10%-20%; The secondary liquid amplification culture (500L tank) of bacillus megaterium, aspergillus niger, bakery yeast; Inoculum concentration 10%-20%; Bacillus megaterium, aspergillus niger, bakery yeast fermentation liquid are mixed according to the following ratio: bacillus megaterium 10%-20%, aspergillus niger 30%-60%, yeast 30%-60%.
5. Chinese medicine extraction liquid and preparation method thereof is as follows: Chinese medicine powder is broken to 60-80 order by (1), takes by formula ratio, and three-dimensional hybrid obtains Chinese medicine powder; (2) mixed Chinese medicine powder, 300-600 part water are put in extraction pot respectively, soak 5-20 hour; (3) be heated to 40-100 DEG C and extract 10-15 hour; (4) collecting by filtration extracting solution; (5) secondary adds 300-600 part water in medicinal residues, and 40-100 DEG C is extracted 10-15 hour; (6) merge extracted twice liquid, 30-70 DEG C is evaporated to original volume 40%-50%.
6. herb fermenting liquid and preparation method thereof is as follows: (1) adds the glucose of 4% in the concentrated liquid of tradition Chinese medicine, and 4% yeast leaching powder, 2% soy peptone, heat up 121 DEG C of sterilizings 30 minutes.(2) with the inoculum concentration of 10%-20% access seed liquor, 30-37 DEG C of bottom fermentation 24 hours.(3) fill.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105327062A (en) * | 2015-10-19 | 2016-02-17 | 施瑞客（天津）生物技术有限公司 | Fermented traditional Chinese medicine preparation for treating yellow head disease of prawns |
| CN109700983A (en) * | 2017-10-26 | 2019-05-03 | 格特生物制药（天津）有限公司 | A kind of fermented tcm preparation for treating bulls neural paralysis |
| CN109700985A (en) * | 2017-10-26 | 2019-05-03 | 格特生物制药（天津）有限公司 | A kind of fermented tcm preparation for treating bulls neural paralysis |
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|---|---|---|---|---|
| CN105327062A (en) * | 2015-10-19 | 2016-02-17 | 施瑞客（天津）生物技术有限公司 | Fermented traditional Chinese medicine preparation for treating yellow head disease of prawns |
| CN109700983A (en) * | 2017-10-26 | 2019-05-03 | 格特生物制药（天津）有限公司 | A kind of fermented tcm preparation for treating bulls neural paralysis |
| CN109700985A (en) * | 2017-10-26 | 2019-05-03 | 格特生物制药（天津）有限公司 | A kind of fermented tcm preparation for treating bulls neural paralysis |
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