CN106387303A - Method for reducing contents of arsenic and mercury in buckwheat protein through adsorption and substitution - Google Patents
Method for reducing contents of arsenic and mercury in buckwheat protein through adsorption and substitution Download PDFInfo
- Publication number
- CN106387303A CN106387303A CN201610803967.2A CN201610803967A CN106387303A CN 106387303 A CN106387303 A CN 106387303A CN 201610803967 A CN201610803967 A CN 201610803967A CN 106387303 A CN106387303 A CN 106387303A
- Authority
- CN
- China
- Prior art keywords
- serosity
- add
- arsenic
- wheat protein
- kilogram
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 31
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 31
- 241000219051 Fagopyrum Species 0.000 title claims abstract description 29
- 235000009419 Fagopyrum esculentum Nutrition 0.000 title claims abstract description 29
- 229910052785 arsenic Inorganic materials 0.000 title claims abstract description 16
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 title claims abstract description 16
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 title claims abstract description 15
- 229910052753 mercury Inorganic materials 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title claims abstract description 10
- 238000006467 substitution reaction Methods 0.000 title claims abstract description 8
- 238000001179 sorption measurement Methods 0.000 title abstract description 5
- 238000002156 mixing Methods 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 5
- 230000001954 sterilising effect Effects 0.000 claims abstract description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 5
- 238000003756 stirring Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 8
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 7
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 6
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 5
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 5
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 4
- WHMDKBIGKVEYHS-IYEMJOQQSA-L Zinc gluconate Chemical compound [Zn+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O WHMDKBIGKVEYHS-IYEMJOQQSA-L 0.000 claims description 4
- 235000010980 cellulose Nutrition 0.000 claims description 4
- 229920002678 cellulose Polymers 0.000 claims description 4
- 239000001913 cellulose Substances 0.000 claims description 4
- 239000013522 chelant Substances 0.000 claims description 4
- 239000003651 drinking water Substances 0.000 claims description 4
- 235000020188 drinking water Nutrition 0.000 claims description 4
- 239000011975 tartaric acid Substances 0.000 claims description 4
- 235000002906 tartaric acid Nutrition 0.000 claims description 4
- 229960000306 zinc gluconate Drugs 0.000 claims description 4
- 235000011478 zinc gluconate Nutrition 0.000 claims description 4
- 239000011670 zinc gluconate Substances 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims 1
- 230000003750 conditioning effect Effects 0.000 abstract 1
- 230000018044 dehydration Effects 0.000 abstract 1
- 238000006297 dehydration reaction Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 21
- 229910001385 heavy metal Inorganic materials 0.000 description 4
- 235000015165 citric acid Nutrition 0.000 description 3
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- -1 tartaric acid-citric acid compound Chemical class 0.000 description 2
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000002079 cooperative effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/18—Vegetable proteins from wheat
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention relates to a method for reducing the contents of arsenic and mercury in buckwheat protein through adsorption and substitution. The method disclosed by the invention comprises the following steps of taking buckwheat protein powder, performing pulp blending, performing chelating, performing adsorption, performing solid-liquid separation, performing substitution, performing conditioning, performing sterilization, performing dehydration, performing drying and the like. According to the method disclosed by the invention, the contents of the arsenic and the mercury in the buckwheat protein can be effectively and synchronously reduced, and besides, the feeling quality and the nutrient value of the buckwheat protein cannot be influenced.
Description
Technical field
The present invention relates to adsorbing arsenic, the mercury content that method of substitution reduces in buck wheat protein, belong to processing of farm products field.
Background technology
Buck wheat protein has good aminoacid composition and ratio, and digestibility is high, is a kind of excellent vegetable protein, has
Important value.Recently as the development of industry, mining industry etc., heavy metal pollution problem progressively exposes.Arsenic, hydrargyrum attachment
In protein, the edible safety of albumen is led to be affected.After enrichment arsenic, the protein of hydrargyrum are eaten, can be in vivo
Enrichment, serious harm health.
Content of the invention
The present invention is intended to provide a kind of method is easy, the rational method of cost reduces arsenic in buck wheat protein, mercury content, carry
High edible safety.
Arsenic in absorption method of substitution reduction buck wheat protein, mercury content, comprise the following steps：
(1) size mixing：Take buckwheat protein powder double centner, add 1200-1220 kilogram of drinking water, mix；
(2) chelate：Add 1.6-1.8 kilogram of tartaric acid and 2.2-2.4 kilogram of citric acid, then adjust serosity pH to 3.6-
3.8, mix, at 42-44 DEG C, keep 2.6-2.8 hour, period is stirred continuously, and mixing speed is 3200-3400 rev/min；
(3) adsorb：Adjust buck wheat protein serosity pH to 4.2-4.4, add 3.0-3.2 kilogram of cellulose, stir at 52-54 DEG C
Mix 46-48 minute, mixing speed is 4400-4600 rev/min；
(4) solid-liquid separation：Carry out solid-liquid separation, remove insoluble solid；
(5) replace：Adjust buck wheat protein serosity pH to 5.6-5.8, add 240-260 gram of zinc gluconate, at 44-46 DEG C
Stirring 64-66 minute, mixing speed is 2200-2400 rev/min；
(6)) quenched：The pH to 6.3-6.5 of buck wheat protein serosity is adjusted in 15 minutes；
(7) sterilize, be dehydrated and be dried：Serosity carries out sterilization treatment, is then dehydrated, is dried, obtains product.
Wherein, cellulose is Microcrystalline Cellulose, and mean diameter is 220-240 mesh.
The present invention has the advantage that：
1st, the present invention adopt tartaric acid-citric acid compound system, the specific acid condition needed for existing highly effective chelating, simultaneously
There is enough molecular radicals for collaborative chelating arsenic, hydrargyrum.
2nd, under specific pH, add particular fineness Microcrystalline Cellulose, can further target heavy metal adsorption out, from
And reach the synchronous effect removing removing heavy metals.Specific mixing speed can ensure that Microcrystalline Cellulose and heavy metal are fully contacted instead
Should, the removal efficiency of lifting arsenic, hydrargyrum under the dynamic equilibrium of absorption-parsing.
3rd, the substitution reaction stage, using zinc ion under specific environment, chelation is replaced to arsenic, the competitive of hydrargyrum, reduce and produce
Arsenic in product, mercury content.
4th, the present invention adopts the highly effective chelating effect of tartaric acid-citric acid compound system, the Microcrystalline Cellulose of particular fineness
Adsorption and specific environment under zinc ion metalepsis, by the cooperative effect of three, synchronous reduce buck wheat protein
In arsenic, mercury content.
Specific embodiment
Describe in more detail the present invention below by specific embodiment.
Embodiment 1
(1) size mixing：Take buckwheat protein powder double centner, add 1200 kilograms of drinking waters, mix；(2) chelate：Add 1.6 thousand
Gram tartaric acid and 2.2 kilograms of citric acids, then adjust serosity pH to 3.6, mix, and keep 2.6 hours, period constantly stirs at 42 DEG C
Mix, mixing speed is 3200 revs/min；(3) adsorb：Adjust buck wheat protein serosity pH to 4.2, add 3.0 kilograms of microcrystalline celluloses
Element (mean diameter is 240 mesh), stirs 46 minutes at 52 DEG C, mixing speed is 4400 revs/min；(4) solid-liquid separation：Carry out solid
Liquid separates, and removes insoluble solid；(5) replace：Regulation buck wheat protein serosity pH to 5.6,240 grams of zinc gluconate of addition, 44
Stir 66 minutes at DEG C, mixing speed is 2400 revs/min；(6)) quenched：The pH of buck wheat protein serosity is adjusted in 15 minutes
To 6.5；(7) sterilize, be dehydrated and be dried：Serosity carries out sterilization treatment, is then dehydrated, is dried, obtains product.
Embodiment 2
(1) size mixing：Take buckwheat protein powder double centner, add 1220 kilograms of drinking waters, mix；(2) chelate：Add 1.8 thousand
Gram tartaric acid and 2.4 kilograms of citric acids, then adjust serosity pH to 3.8, mix, and keep 2.8 hours, period constantly stirs at 44 DEG C
Mix, mixing speed is 3400 revs/min；(3) adsorb：Adjust buck wheat protein serosity pH to 4.4, add 3.2 kilograms of microcrystalline celluloses
Element (mean diameter is 240 mesh), stirs 46 minutes at 54 DEG C, mixing speed is 4400 revs/min；(4) solid-liquid separation：Carry out solid
Liquid separates, and removes insoluble solid；(5) replace：Regulation buck wheat protein serosity pH to 5.6,240 grams of zinc gluconate of addition, 44
Stir 64 minutes at DEG C, mixing speed is 2200 revs/min；(6)) quenched：The pH of buck wheat protein serosity is adjusted in 15 minutes
To 6.3；(7) sterilize, be dehydrated and be dried：Serosity carries out sterilization treatment, is then dehydrated, is dried, obtains product.
After testing, the arsenic of embodiment 1 and embodiment 2 buck wheat protein product, mercury content all decline more than 85%, product simultaneously
Mode of appearance and organoleptic quality be substantially not changed in.
Claims (2)
1. the arsenic in absorption method of substitution reduction buck wheat protein, mercury content are it is characterised in that comprise the following steps：
(1) size mixing：Take buckwheat protein powder double centner, add 1200-1220 kilogram of drinking water, mix；
(2) chelate：Add 1.6-1.8 kilogram of tartaric acid and 2.2-2.4 kilogram of citric acid, then adjust serosity pH to 3.6-3.8,
Mix, at 42-44 DEG C, keep 2.6-2.8 hour, period is stirred continuously, and mixing speed is 3200-3400 rev/min；
(3) adsorb：Adjust buck wheat protein serosity pH to 4.2-4.4, add 3.0-3.2 kilogram of cellulose, stir at 52-54 DEG C
46-48 minute, mixing speed is 4400-4600 rev/min；
(4) solid-liquid separation：Carry out solid-liquid separation, remove insoluble solid；
(5) replace：Adjust buck wheat protein serosity pH to 5.6-5.8, add 240-260 gram of zinc gluconate, stir at 44-46 DEG C
64-66 minute, mixing speed is 2200-2400 rev/min；
(6)) quenched：The pH to 6.3-6.5 of buck wheat protein serosity is adjusted in 15 minutes；
(7) sterilize, be dehydrated and be dried：Serosity carries out sterilization treatment, is then dehydrated, is dried, obtains product.
2. it is characterised in that cellulose is Microcrystalline Cellulose, mean diameter is production method according to claim 1
220-240 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610803967.2A CN106387303A (en) | 2016-09-06 | 2016-09-06 | Method for reducing contents of arsenic and mercury in buckwheat protein through adsorption and substitution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610803967.2A CN106387303A (en) | 2016-09-06 | 2016-09-06 | Method for reducing contents of arsenic and mercury in buckwheat protein through adsorption and substitution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106387303A true CN106387303A (en) | 2017-02-15 |
Family
ID=57998353
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610803967.2A Pending CN106387303A (en) | 2016-09-06 | 2016-09-06 | Method for reducing contents of arsenic and mercury in buckwheat protein through adsorption and substitution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106387303A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107098966A (en) * | 2017-06-20 | 2017-08-29 | 华兰生物工程股份有限公司 | A kind of immunoglobulin and preparation method thereof |
| CN107236038A (en) * | 2017-06-20 | 2017-10-10 | 华兰生物工程股份有限公司 | Microcrystalline cellulose improves method of protein clarity and products thereof and application |
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|---|---|---|---|---|
| CN1974432A (en) * | 2005-12-02 | 2007-06-06 | 东海生物科技股份有限公司 | Use of gamma- polyglutamic acid, gamma- polyglutamate and/ or its aqueous gel for removing heavy metal and dissolving calcium and/ or magnesium scale |
| CN101274268A (en) * | 2007-12-29 | 2008-10-01 | 中国科学院长春应用化学研究所 | Biological adsorbing agent with adsorption capacity of heavy metal ion, and producing method and application thereof |
| CN103007896A (en) * | 2012-12-24 | 2013-04-03 | 漳州师范学院 | Preparation method of cellulose adsorbent for adsorbing heavy metal cations |
| CN103340414A (en) * | 2013-07-12 | 2013-10-09 | 普洱永吉生物技术有限责任公司 | Method for removing cadmium in rice protein |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107098966A (en) * | 2017-06-20 | 2017-08-29 | 华兰生物工程股份有限公司 | A kind of immunoglobulin and preparation method thereof |
| CN107236038A (en) * | 2017-06-20 | 2017-10-10 | 华兰生物工程股份有限公司 | Microcrystalline cellulose improves method of protein clarity and products thereof and application |
| CN107098966B (en) * | 2017-06-20 | 2021-03-30 | 华兰生物工程股份有限公司 | Immunoglobulin and preparation method thereof |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| RJ01 | Rejection of invention patent application after publication | ||
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Application publication date: 20170215 |