CN107047924B - Method for preparing heavy metal-removed pea protein from pea starch wastewater - Google Patents
Method for preparing heavy metal-removed pea protein from pea starch wastewater Download PDFInfo
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- CN107047924B CN107047924B CN201710244366.7A CN201710244366A CN107047924B CN 107047924 B CN107047924 B CN 107047924B CN 201710244366 A CN201710244366 A CN 201710244366A CN 107047924 B CN107047924 B CN 107047924B
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- 108010084695 Pea Proteins Proteins 0.000 title claims abstract description 84
- 235000019702 pea protein Nutrition 0.000 title claims abstract description 84
- 229920002472 Starch Polymers 0.000 title claims abstract description 24
- 239000008107 starch Substances 0.000 title claims abstract description 24
- 235000019698 starch Nutrition 0.000 title claims abstract description 24
- 239000002351 wastewater Substances 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 16
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 49
- 238000000855 fermentation Methods 0.000 claims abstract description 31
- 230000004151 fermentation Effects 0.000 claims abstract description 31
- 229920002749 Bacterial cellulose Polymers 0.000 claims abstract description 21
- 239000005016 bacterial cellulose Substances 0.000 claims abstract description 21
- 150000007524 organic acids Chemical class 0.000 claims abstract description 15
- 241000589232 Gluconobacter oxydans Species 0.000 claims abstract description 9
- 241001052560 Thallis Species 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims description 40
- 239000002244 precipitate Substances 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 235000002837 Acetobacter xylinum Nutrition 0.000 claims description 20
- 239000006228 supernatant Substances 0.000 claims description 19
- 238000003756 stirring Methods 0.000 claims description 15
- 239000000243 solution Substances 0.000 claims description 12
- 239000003513 alkali Substances 0.000 claims description 10
- 239000012295 chemical reaction liquid Substances 0.000 claims description 8
- 239000002002 slurry Substances 0.000 claims description 8
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 5
- 238000010907 mechanical stirring Methods 0.000 claims description 5
- 230000007935 neutral effect Effects 0.000 claims description 5
- 230000003472 neutralizing effect Effects 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000009423 ventilation Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 235000005985 organic acids Nutrition 0.000 claims description 4
- 239000012460 protein solution Substances 0.000 claims description 4
- 241001136169 Komagataeibacter xylinus Species 0.000 claims 3
- 238000007670 refining Methods 0.000 abstract description 2
- 238000003916 acid precipitation Methods 0.000 abstract 1
- 235000010582 Pisum sativum Nutrition 0.000 description 19
- 241000219843 Pisum Species 0.000 description 18
- 244000235858 Acetobacter xylinum Species 0.000 description 17
- 235000018102 proteins Nutrition 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 239000000843 powder Substances 0.000 description 5
- 229910052793 cadmium Inorganic materials 0.000 description 4
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 4
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 4
- 229910052753 mercury Inorganic materials 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000001694 spray drying Methods 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000002009 allergenic effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/16—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste water of starch-manufacturing plant or like wastes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Processing Of Solid Wastes (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A method for preparing pea protein without heavy metals from pea starch wastewater by using a fermentation method comprises the steps of fermenting the pea starch wastewater by using acetobacter gluconicum to generate organic acid for chelating the first heavy metals, adsorbing the second heavy metals in the pea protein by using acetobacter gluconicum thalli and bacterial cellulose generated by the acetobacter gluconicum, and finally carrying out acid precipitation to elute the third heavy metals in the pea protein in the refining process of the pea protein. According to the invention, the pea protein is refined from the pea starch wastewater by a fermentation method, and heavy metals in the pea protein are removed in three steps, so that the pea protein with high protein content and standard heavy metals is obtained.
Description
Technical Field
The invention relates to the technical field of food processing, in particular to a method for refining pea protein from pea starch wastewater by a fermentation method and removing heavy metals in the pea protein in three steps to obtain pea protein with high protein content and standard heavy metals.
Background
Pea protein is a high-quality protein, the proportion of amino acid is relatively balanced, the digestion utilization rate is more than 98%, and the eight essential amino acids required by human bodies reach FAO/WHO recommended model values except the slightly low content of methionine. Pea protein is non-allergenic protein and does not contain anti-nutritional factors, so that the pea protein per se has higher added value. Can be widely applied to health products, meat products, beverages, baked foods, dairy products and the like. Legumes do not contain cholesterol, which is superior to animal proteins.
The pea starch production needs to consume a large amount of water, and the wastewater is discharged, thereby not only wasting protein resources, but also causing serious pollution to the environment. The recovery of pea protein from pea starch is necessary or would result in a great waste. In the early 90 s of the last century, a crude method of natural precipitation and large-boiler boiling was invented to recover pea protein. However, this method has the great disadvantage that natural precipitation takes a long time and is liable to cause protein putrefaction, especially in summer, which not only reduces the nutritional value of the protein, but also pollutes the surrounding air; pea protein obtained by boiling in a big pot is denatured at high temperature, and the physicochemical property is poor.
At present, a few companies adopt a spray drying method to recover protein in wastewater, so that the waste of the protein is reduced. However, in the materials recovered by adopting the spray drying method, the pea protein has poor functionality due to the denaturation of the protein, and the recovered protein has low content, can only be added into feed as pea meal, and has extremely low additional value, thereby causing the waste of protein resources.
The problem of heavy metal pollution of peas is gradually exposed due to agricultural pollution of the industry and mining industry. Cadmium, lead, mercury and the like are combined with pea protein in a complex form, so that heavy metals in the pea protein are enriched, and the edible safety of the pea protein is influenced.
Disclosure of Invention
In view of the above problems of the prior art, the present application provides a method for preparing heavy metal-removed pea protein from pea starch wastewater. According to the invention, the fermentation process is combined with the pea protein preparation process, and the refined pea protein without heavy metals can be obtained by utilizing the fermentation process and the fermentation product, so that the defect that the existing pea protein heavy metal removal process is complex is overcome.
The technical scheme of the invention is as follows:
a method for preparing pea protein without heavy metals from pea starch wastewater comprises the following specific steps:
(1) instantly sterilizing pea starch wastewater solution under high pressure, feeding the pea starch wastewater solution into a mechanical stirring ventilation fermentation tank, inoculating 5-20% of gluconacetobacter xylinus seeds, fermenting for 5-96 h at the temperature of 25-37 ℃, and obtaining pea starch wastewater fermentation liquor containing micelle-like bacterial cellulose and organic acid, wherein the pH of the fermentation liquor is 4.0-4.8 after the fermentation is finished;
(2) separating the fermentation liquor by using a centrifugal machine, wherein the centrifugal force is 2500-;
(3) conveying the precipitate obtained in the step (2) into a size mixing tank, adding 1-3 times of water for size mixing, adding an alkali liquor for adjusting the pH value to 8.0-13.0, stirring at the temperature of 40-50 ℃ at the speed of 50-70r/min, reacting for 20-80 min, and adsorbing heavy metals in pea protein by using gluconacetobacter xylinus and bacterial cellulose generated by the gluconacetobacter xylinus while dissolving the pea protein;
(4) separating the reaction solution prepared in the step (3) by using a centrifugal machine, wherein the centrifugal force is 2500-;
(5) sending the supernatant prepared in the step (4) into a size mixing tank, adding 1-3 times of water for size mixing, adding acid liquor to adjust the pH value to 4.0-4.8, stirring at the temperature of 40-45 ℃ at the speed of 50-70r/min, reacting for 20-60 min, and eluting heavy metals in pea protein while obtaining the pea protein;
(6) separating the reaction liquid prepared in the step (5) by using a centrifugal machine, wherein the centrifugal force is 2500-;
(7) delivering the pea protein prepared in the step (6) into a size mixing tank, adding 1-3 times of water for size mixing, washing for 10-30 min at the temperature of 30-35 ℃, stirring at the speed of 50-70r/min, separating by using a centrifugal machine, and obtaining the pea protein with the centrifugal force of 2500-;
(8) and (3) delivering the pea protein prepared in the step (7) into a size mixing tank, adding 1-3 times of water for size mixing, adding an alkali liquor for adjusting the pH value to be neutral, neutralizing at the temperature of 30-35 ℃ for 10-30 min, and separating by using a centrifugal machine, wherein the centrifugal force is (2500 + 3000) x g to obtain the pea protein.
The heavy metal is lead, cadmium or mercury. The concentration of heavy metals in the pea starch wastewater is not more than 3.0mg/L, namely the concentration of lead is not more than 1.0mg/L, the concentration of cadmium is not more than 1.0mg/L, and the concentration of mercury is not more than 1.0 mg/L.
The beneficial technical effects of the invention are as follows:
the bacterial cellulose is generated by fermenting the gluconacetobacter xylinus, on one hand, the bacterial cellulose can well adsorb heavy metals, on the other hand, the thalli of the gluconacetobacter xylinus, whether dead bacteria or live bacteria, can adsorb the heavy metals, and moreover, the gluconacetobacter xylinus is fermented to generate organic acid which can chelate the heavy metals.
Detailed Description
The present invention will be described in detail with reference to examples.
Example 1:
(1) instantly sterilizing pea starch wastewater solution under high pressure, feeding into a mechanical stirring ventilation fermentation tank, inoculating 5% of acetobacter xylinum seeds, fermenting at 25 ℃ for 96 hours, and obtaining pea starch wastewater fermentation liquor containing micelle-like bacterial cellulose and organic acid, wherein the pH of the fermentation liquor is 4.8 after the fermentation is finished;
(2) separating the fermentation liquor by a centrifugal machine, wherein the centrifugal force is 3000 Xg, and obtaining supernatant and precipitate, wherein the supernatant is fermented organic acid liquor chelated with heavy metals, and the precipitate is pea protein chelated with organic acids to remove the heavy metals for the first time, acetobacter xylinum thalli and bacterial cellulose;
(3) conveying the precipitate obtained in the step (2) into a size mixing tank, adding 3 times of water for size mixing, adding an alkali liquor for adjusting the pH to 13.0, stirring at the temperature of 45-48 ℃ at the speed of 70r/min, reacting for 80min, and adsorbing heavy metals in pea protein by using the acetobacter xylinum thalli and bacterial cellulose generated by the acetobacter xylinum thalli while dissolving the pea protein;
(4) separating the reaction solution prepared in the step (3) by using a centrifugal machine, wherein the centrifugal force is 3000 Xg, and obtaining supernatant and precipitate, wherein the supernatant is pea protein solution without heavy metals twice, and the precipitate is acetobacter gluconicum thallus and bacterial cellulose which absorb the heavy metals;
(5) sending the supernatant prepared in the step (4) into a slurry mixing tank, adding 3 times of water for mixing slurry, adding acid liquor for regulating the pH to 4.8, stirring at the temperature of 43-45 ℃ at the speed of 70r/min, reacting for 60min, and eluting heavy metals in pea protein while obtaining the pea protein;
(6) separating the reaction liquid prepared in the step (5) by using a centrifugal machine, wherein the centrifugal force is 3000 Xg, and obtaining pea protein without the heavy metals for three times;
(7) delivering the pea protein prepared in the step (6) into a size mixing tank, adding 3 times of water for size mixing, washing for 30min at the temperature of 35 ℃, stirring at the speed of 70r/min, separating by using a centrifugal machine, and obtaining the pea protein with the centrifugal force of 3000 Xg;
(8) and (3) feeding the pea protein prepared in the step (7) into a size mixing tank, adding 3 times of water for size mixing, adding an alkali liquor for adjusting the pH value to be neutral, neutralizing for 30min at the temperature of 35 ℃, and separating by using a centrifugal machine, wherein the centrifugal force is 3000 Xg to obtain the pea protein.
Example 2:
(1) instantly sterilizing pea starch wastewater solution under high pressure, feeding the pea starch wastewater solution into a mechanical stirring ventilation fermentation tank, inoculating 20% of acetobacter xylinum seeds, fermenting for 5 hours at 37 ℃, and obtaining pea starch wastewater fermentation liquor containing micelle-like bacterial cellulose and organic acid, wherein the pH value of the fermentation liquor is 4.0-4.8 after the fermentation is finished;
(2) separating the fermentation liquor by a centrifugal machine, wherein the centrifugal force is 2500 Xg, so as to obtain supernatant and precipitate, wherein the supernatant is fermented organic acid liquor chelated with heavy metals, and the precipitate is pea protein chelated with organic acids to remove the heavy metals for the first time, acetobacter xylinum and bacterial cellulose;
(3) conveying the precipitate obtained in the step (2) into a size mixing tank, adding 1 time of water for size mixing, adding an alkali liquor for adjusting the pH to 8.5, stirring at 40 ℃ at a speed of 50r/min, reacting for 25min, and adsorbing heavy metals in pea protein by using the gluconacetobacter xylinus and bacterial cellulose generated by the gluconacetobacter xylinus while dissolving the pea protein;
(4) separating the reaction liquid prepared in the step (3) by using a centrifugal machine, wherein the centrifugal force is 2500g, and obtaining supernate and precipitate, wherein the supernate is pea protein solution without heavy metals twice, and the precipitate is acetobacter gluconicum thallus and bacterial cellulose which absorb the heavy metals;
(5) sending the supernatant prepared in the step (4) into a slurry mixing tank, adding 1 time of water for mixing slurry, adding acid solution for regulating the pH to 4.2, stirring at 40 ℃ and 50r/min for reaction for 20min, and eluting heavy metals in pea protein while obtaining the pea protein;
(6) separating the reaction liquid prepared in the step (5) by using a centrifugal machine, wherein the centrifugal force is 2500 Xg, and obtaining pea protein without the heavy metals for three times;
(7) delivering the pea protein prepared in the step (6) into a size mixing tank, adding 1 time of water for size mixing, washing for 10min at the temperature of 30 ℃, stirring at the speed of 50r/min, and separating by using a centrifugal machine, wherein the centrifugal force is 2500 Xg to obtain the pea protein;
(8) and (3) feeding the pea protein prepared in the step (7) into a size mixing tank, adding 1 time of water for size mixing, adding an alkali liquor for adjusting the pH value to be neutral, neutralizing at the temperature of 30 ℃ for 10min, and separating by using a centrifugal machine, wherein the centrifugal force is 2500 Xg to obtain the pea protein.
Example 3:
(1) instantly sterilizing pea starch wastewater solution under high pressure, feeding into a mechanical stirring ventilation fermentation tank, inoculating 10% of acetobacter xylinum seeds, fermenting at 20 ℃ for 48 hours, and obtaining pea starch wastewater fermentation liquor containing micelle-like bacterial cellulose and organic acid, wherein the pH of the fermentation liquor is 4.4 after the fermentation is finished;
(2) separating the fermentation liquor by a centrifugal machine, wherein the centrifugal force is 2800 Xg, and obtaining supernatant and precipitate, wherein the supernatant is fermented organic acid liquor chelated with heavy metals, and the precipitate is pea protein chelated with organic acids to remove the heavy metals for the first time, acetobacter gluconicum thallus and bacterial cellulose;
(3) conveying the precipitate obtained in the step (2) into a size mixing tank, adding 2 times of water for size mixing, adding an alkali liquor for regulating the pH to 10.0, stirring at 45 ℃ for 60r/min, reacting for 60min, and adsorbing heavy metals in pea protein by using the acetobacter xylinum thalli and bacterial cellulose generated by the acetobacter xylinum thalli while dissolving the pea protein;
(4) separating the reaction liquid prepared in the step (3) by using a centrifugal machine, wherein the centrifugal force is 2800 Xg, and obtaining supernatant and precipitate, wherein the supernatant is pea protein solution from which heavy metals are removed twice, and the precipitate is heavy metal-adsorbed acetobacter gluconicum thallus and bacterial cellulose;
(5) sending the supernatant prepared in the step (4) into a slurry mixing tank, adding 2 times of water for mixing slurry, adding acid solution for regulating the pH to 4.5, stirring at 42 ℃ and 60r/min for reacting for 40min, and eluting heavy metals in pea protein while obtaining the pea protein;
(6) separating the reaction liquid prepared in the step (5) by using a centrifugal machine, wherein the centrifugal force is 2800 Xg, and obtaining pea protein without heavy metals for three times;
(7) delivering the pea protein prepared in the step (6) into a size mixing tank, adding 2 times of water for size mixing, washing for 20min at the temperature of 32 ℃, stirring at the speed of 60r/min, separating by using a centrifugal machine, and obtaining the pea protein with the centrifugal force of 2800 Xg;
(8) and (3) feeding the pea protein prepared in the step (7) into a size mixing tank, adding 2 times of water for size mixing, adding an alkali liquor for adjusting the pH value to be neutral, neutralizing at the temperature of 32 ℃ for 20min, and separating by using a centrifugal machine, wherein the centrifugal force is 2800 Xg to obtain the pea protein.
Test example:
the performance analysis of examples 1-3 and comparative examples is shown in Table 1.
TABLE 1
Note: the higher the score for sensory quality, the better the taste.
Compared with a certain brand of pea protein powder, the pea protein powder prepared by the three examples has higher quality than a reference product and lower heavy metal content. Particularly, the content of lead, cadmium and mercury in the pea protein powder prepared in the third embodiment is 0.078mg/kg, 0.018mg/kg and 0.056mg/kg respectively, the heavy metal content meets the national standard, and the pea protein powder prepared in the third embodiment is lower than that of pea protein powder of the brand, and has fine taste and better evaluation result.
The specific embodiments described herein are merely illustrative of the spirit and some of the experiments performed. Various modifications or additions may be made or substituted in a similar manner to the specific embodiments described herein by those skilled in the art without departing from the spirit of the invention or exceeding the scope thereof as defined in the appended claims.
Claims (1)
1. A method for reducing heavy metals in pea starch wastewater is characterized by comprising the following specific steps:
(1) instantly sterilizing pea starch wastewater solution under high pressure, feeding into a mechanical stirring ventilation fermentation tank, inoculating 10% of acetobacter xylinum seeds, fermenting at 20 ℃ for 48 hours, and obtaining pea starch wastewater fermentation liquor containing micelle-like bacterial cellulose and organic acid, wherein the pH of the fermentation liquor is 4.4 after the fermentation is finished;
(2) separating the fermentation liquor by a centrifugal machine, wherein the centrifugal force is 2800 Xg, and obtaining supernatant and precipitate, wherein the supernatant is fermented organic acid liquor chelated with heavy metals, and the precipitate is pea protein chelated with organic acids to remove the heavy metals for the first time, acetobacter gluconicum thallus and bacterial cellulose;
(3) conveying the precipitate obtained in the step (2) into a size mixing tank, adding 2 times of water for size mixing, adding an alkali liquor for regulating the pH to 10.0, stirring at 45 ℃ for 60r/min, reacting for 60min, and adsorbing heavy metals in pea protein by using the acetobacter xylinum thalli and bacterial cellulose generated by the acetobacter xylinum thalli while dissolving the pea protein;
(4) separating the reaction liquid prepared in the step (3) by using a centrifugal machine, wherein the centrifugal force is 2800 Xg, and obtaining supernatant and precipitate, wherein the supernatant is pea protein solution from which heavy metals are removed twice, and the precipitate is heavy metal-adsorbed acetobacter gluconicum thallus and bacterial cellulose;
(5) sending the supernatant prepared in the step (4) into a slurry mixing tank, adding 2 times of water for mixing slurry, adding acid solution for regulating the pH to 4.5, stirring at 42 ℃ and 60r/min for reacting for 40min, and eluting heavy metals in pea protein while obtaining the pea protein;
(6) separating the reaction liquid prepared in the step (5) by using a centrifugal machine, wherein the centrifugal force is 2800 Xg, and obtaining pea protein without heavy metals for three times;
(7) delivering the pea protein prepared in the step (6) into a size mixing tank, adding 2 times of water for size mixing, washing for 20min at the temperature of 32 ℃, stirring at the speed of 60r/min, separating by using a centrifugal machine, and obtaining the pea protein with the centrifugal force of 2800 Xg;
(8) and (3) feeding the pea protein prepared in the step (7) into a size mixing tank, adding 2 times of water for size mixing, adding an alkali liquor for adjusting the pH value to be neutral, neutralizing at the temperature of 32 ℃ for 20min, and separating by using a centrifugal machine, wherein the centrifugal force is 2800 Xg to obtain the pea protein.
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| CN1850636A (en) * | 2006-05-19 | 2006-10-25 | 东华大学 | Method for preparing bacterial cellulose suspension for efficient rapid heavy metal ion adsorption |
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Denomination of invention: A Method for Preparing Pea Protein with Heavy Metal Removal from Pea Starch Wastewater Effective date of registration: 20230828 Granted publication date: 20210219 Pledgee: Agricultural Bank of China Limited by Share Ltd. Wuxi Huishan branch Pledgor: WUXI JINNONG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023980053750 |