CN106386704A - Method of breeding artemia in high density under alga cultivation environment - Google Patents
Method of breeding artemia in high density under alga cultivation environment Download PDFInfo
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- CN106386704A CN106386704A CN201610784048.5A CN201610784048A CN106386704A CN 106386704 A CN106386704 A CN 106386704A CN 201610784048 A CN201610784048 A CN 201610784048A CN 106386704 A CN106386704 A CN 106386704A
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- 241001247197 Cephalocarida Species 0.000 title claims abstract description 69
- 238000009395 breeding Methods 0.000 title claims abstract description 25
- 230000001488 breeding effect Effects 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title abstract description 15
- 241000195493 Cryptophyta Species 0.000 claims abstract description 61
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 15
- 239000001301 oxygen Substances 0.000 claims abstract description 15
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 8
- 239000013535 sea water Substances 0.000 claims description 25
- 239000001963 growth medium Substances 0.000 claims description 22
- 230000002421 anti-septic effect Effects 0.000 claims description 18
- 150000003839 salts Chemical class 0.000 claims description 17
- 238000005273 aeration Methods 0.000 claims description 15
- 238000012258 culturing Methods 0.000 claims description 9
- 230000000050 nutritive effect Effects 0.000 claims description 6
- 208000031513 cyst Diseases 0.000 claims description 5
- 239000007789 gas Substances 0.000 claims description 5
- 230000012447 hatching Effects 0.000 claims description 5
- 239000004575 stone Substances 0.000 claims description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 4
- 241001474374 Blennius Species 0.000 claims description 4
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 4
- 241000238582 Artemia Species 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 2
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 2
- 229960002685 biotin Drugs 0.000 claims description 2
- 235000020958 biotin Nutrition 0.000 claims description 2
- 239000011616 biotin Substances 0.000 claims description 2
- KYQODXQIAJFKPH-UHFFFAOYSA-N diazanium;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [NH4+].[NH4+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O KYQODXQIAJFKPH-UHFFFAOYSA-N 0.000 claims description 2
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 claims description 2
- 235000002867 manganese chloride Nutrition 0.000 claims description 2
- 239000011565 manganese chloride Substances 0.000 claims description 2
- 229940099607 manganese chloride Drugs 0.000 claims description 2
- 238000005259 measurement Methods 0.000 claims description 2
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 2
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 2
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 2
- 235000015393 sodium molybdate Nutrition 0.000 claims description 2
- 239000011684 sodium molybdate Substances 0.000 claims description 2
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 2
- 235000010344 sodium nitrate Nutrition 0.000 claims description 2
- 239000004317 sodium nitrate Substances 0.000 claims description 2
- 239000011691 vitamin B1 Substances 0.000 claims description 2
- 239000011715 vitamin B12 Substances 0.000 claims description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 2
- 229960001763 zinc sulfate Drugs 0.000 claims description 2
- 241000206751 Chrysophyceae Species 0.000 claims 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 1
- 239000004115 Sodium Silicate Substances 0.000 claims 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims 1
- 238000005660 chlorination reaction Methods 0.000 claims 1
- 229910017052 cobalt Inorganic materials 0.000 claims 1
- 239000010941 cobalt Substances 0.000 claims 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims 1
- 229910052802 copper Inorganic materials 0.000 claims 1
- 239000010949 copper Substances 0.000 claims 1
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims 1
- 229910052911 sodium silicate Inorganic materials 0.000 claims 1
- 229910052717 sulfur Inorganic materials 0.000 claims 1
- 239000011593 sulfur Substances 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 13
- 238000009360 aquaculture Methods 0.000 abstract 1
- 244000144974 aquaculture Species 0.000 abstract 1
- 239000008399 tap water Substances 0.000 description 5
- 235000020679 tap water Nutrition 0.000 description 5
- 241000133262 Nauplius Species 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 229910052736 halogen Inorganic materials 0.000 description 4
- 150000002367 halogens Chemical class 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 241000238557 Decapoda Species 0.000 description 2
- 241001501873 Isochrysis galbana Species 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 241000965254 Apostichopus japonicus Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000371997 Eriocheir sinensis Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- 229940097267 cobaltous chloride Drugs 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 230000027326 copulation Effects 0.000 description 1
- 210000001320 hippocampus Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000034004 oogenesis Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a method of breeding artemia in high density under alga cultivation environment. In an artemia cultivation water environment, the density of algae is one million cells/mL to ten million cells/mL; the water environment contains pseudomonas aeruginosa whose concentration is 10<6> to 10<8>/mL; the density of artemia is 5 to 12/mL; and the optimized density of artemia is 5 to 10/mL. The method increases dissolved oxygen in water and not only solves the problem of initial feed of artemia, but also solves the problem of low survival rate and spawning rate of high density cultivation. The method is also suitable in large aquaculture pond for guaranteeing stable water environment and survival rate.
Description
Technical field
The invention belongs to high-density breeding artemia technical field, especially relate to artemia high density under a breeding algal rim border and support
The method grown.
Background technology
Artemia " shrimps " the big artemia that is otherwise known as is generally shrimp, Eriocheir sinensiss, Hippocampus, the preferred bait of Stichopus japonicuss.General on market
There is no the such life food of big artemia, only artemia frost body, the naupliuss hatching became big through of short duration one week
Artemia, not only solves the problems, such as that artemia high density survival rate is low and culturing time is long, also solves enterprise and buys the big halogen of frost
The Financial cost of worm and the frost stale problem of big artemia.But at present, people are to bait needed for artemia high-density breeding
Have different opinions, therefore to artemia high-density breeding aspect, not yet it has been proposed that the different impact to artemia high-density breeding for the bait,
By upgrowth situation in different algaes for the artemia, thus finding artemia cultural method rapidly and efficiently, thus cost-effective.
Content of the invention
In view of this, it is contemplated that the method proposing artemia high-density breeding under a breeding algal rim border, this method is not
But achieve the growth of algae, carrying out providing guarantee for artemia oxygen demand under high density while photosynthesis produce oxygen, solution
Determine artemia because of the slow problem of bait hypotrophy, and has ensured that artemia has high survival rate simultaneously, the present invention also has been adapted for
Big cultivation place ensures stable survival rate.
For reaching above-mentioned purpose, the technical scheme is that and be achieved in that：
The method of artemia high-density breeding under one breeding algal rim border, in the water environment of artemia culture, the density of algae is 1,000,000
Individual cell/mL～10,000,000 cell/mL, and water environment includes Pseudomonas aeruginosa, concentration is 106～108Individual/mL, artemia
Density be 5～12/mL；Preferably, the density of artemia is 5～10/mL.
Preferably, the salinity of water environment is 25～35g dissolving salt/L, and pH is 8 to 10, and the concentration of dissolved oxygen is more than 6mg/L,
Water temperature is 22～30 DEG C.
Preferably, in the water environment of artemia culture, the density of artemia is 10/mL.
Preferably, added with the nutritive salt of algae in described water environment, water environment for antiseptic sea water and algae nutritive salt configure and
Become.
Preferably, the nutritive salt of the algae in every liter of sea water includes following component, sodium nitrate 75mg, sodium dihydrogen phosphate 5mg, silicon
Sour sodium 30mg, zinc sulfate 0.023mg, copper sulfate 0.01mg, manganese chloride 0.012mg, iron chloride 3.16mg, sodium molybdate 0.07mg,
B diammonium edta sodium 4.36mg, cobaltous chloride 0.012mg, vitamin B120.5mg, biotin 0.5mg, vitamin B10.1mg.
Preferably, described algae is Isochrysis galbana or hailian seaweed；Described artemia is Bohai Sea Gulf artemia.
Preferably, comprise the steps：
1), the configuration of algae solution：Algae kind is inoculated in the antiseptic sea water added with f/2 culture medium in superclean bench, is placed on
Cultivate in incubator；Preferably, the salinity of antiseptic sea water is 30 ‰；；
2), treat 1,000,000 cell/mL～10,000,000 cell/mL of density of algae, algae solution moved on to culturing jar, simultaneously plus
Enter gas stone and carry out aeration；
3), the hatching of artemia, artemia cysts are poured in hatching pipe, are subsequently adding the sea water that salinity is 20 ‰, and aeration 20～
Hatched within 26 hours；Preferably aeration 24 hours；
4), the cultivation of artemia
A) artemia is put into after algae solution, the meltage in measurement water is so as to be not less than 6mg/L；
B) add Pseudomonas aeruginosa, then added once at interval of 24 hours；
C) interval adds an algae media for two days, adds the culture medium of amount completely for the first time, second and third time
1/2 addition according to the culture medium of amount completely.
With respect to prior art, the method for artemia high-density breeding under a breeding algal rim border of the present invention, have with
Lower advantage：
1) present invention achieves in algae solution middle-high density artemia culture ovum it is ensured that artemia has high survival rate, counting after 7 days
To more than 89%, during solving the problems, such as artemia high-density breeding, survival rate is relatively low for the survival rate calculating artemia.
2) dissolved oxygen that the inventive method not only achieves in water rises, and solves the problems, such as artemia open-mouthed bait, and simultaneously
Shorten the low problem of the spawning rate of the survival rate sum of high-density breeding, the present invention also is adapted for ensureing to stablize in large-scale cultivating pool
Water environment and survival rate.
3) under suitable condition, promote artemia, at one week, copulation and oogenesis phenomenon occur, solve the artemia speed of growth
Slower problem.
Specific embodiment
It should be noted that in the case of not conflicting, the embodiment in the present invention and the feature in embodiment can phases
Mutually combine.
To describe the present invention below in conjunction with embodiment in detail.
Embodiment one
Adding 10L density using 20L clear glass cylinder is 2000000/ml algae solution aeration, is subsequently adding 100000 halogen
Worm naupliuss, add the culture medium of algae according to the proportioning of f/2 culture medium, the 3rd day according to 5L algae simultaneously to the amount of 10L algae
Amount adds the culture medium of algae, also adds culture medium to be cultivated according to 5L algae within the 6th day.4 points of every afternoon adds 25ml concentration
For 109The Pseudomonas aeruginosa of individual/mL, every 24 hours add once, its salinity be 30g dissolving salt/L, pH be 8.5 to 9.6 it
Between fluctuate, the concentration of dissolved oxygen is more than 6mg/L, and water temperature is 26 ± 2 DEG C；
The configuration process of algae solution is to add f/2 culture medium in antiseptic sea water, then inoculate algae kind in superclean bench
To in the antiseptic sea water added with f/2 culture medium, it is placed on culture in incubator；When the density of algae is 2000000/ml, by algae
Liquid pours culturing jar into, is simultaneously introduced gas stone and carries out aeration, the salinity of antiseptic sea water is 30 ‰；Described antiseptic sea water can be by sea
Water forms using it is also possible to be allocated with tap water allotment salt after carrying out sterilization treatment.
Described algae is Isochrysis galbana；And use Bohai Sea Gulf artemia cysts.
After one week, artemia survival rate rate reaches 89%, and the body length of artemia averagely increased 3.2mm.
In culturing jar, after aeration, dissolved oxygen, in 6.65mg/L, then gradually rises, and dissolved oxygen can reach within the 4th day
9.1mg/L.
Embodiment two
Adding 10L density using 20L clear glass cylinder is 2000000/ml algae solution aeration, is subsequently adding 100000 halogen
Worm naupliuss, add the culture medium of algae according to the amount of 10L algae, the amount according to 5L algae adds the culture medium of algae within the 3rd day simultaneously,
Also culture medium is added to be cultivated according to 5L algae within 6th day.Add the Pseudomonas aeruginosa of 25ml every night, algae solution is to pass through
Tap water allotment salt allotment forms, and its salinity is 30g dissolving salt/L, and pH is to fluctuate between 8.5 to 9.6, the concentration of dissolved oxygen
More than 6mg/L, water temperature is 26 ± 2 DEG C；
The configuration process of algae solution is to add f/2 culture medium in antiseptic sea water, then inoculate algae kind in superclean bench
To in the antiseptic sea water added with f/2 culture medium, it is placed on culture in incubator；When the density of algae is 2000000/ml, by algae
Liquid pours culturing jar into, is simultaneously introduced gas stone and carries out aeration, the salinity of antiseptic sea water is 30 ‰；Described antiseptic sea water can be by sea
Water forms using it is also possible to be allocated with tap water allotment salt after carrying out sterilization treatment.
Described algae is hailian seaweed；And use Bohai Sea Gulf artemia cysts.
After one week, artemia survival rate rate reaches 94%, and the body length of artemia averagely increased 2.5mm.
In culturing jar, after aeration, dissolved oxygen, in 6.75mg/L, then gradually rises, and dissolved oxygen can reach within the 4th day
9.3mg/L.
Embodiment three
Adding 50L density using 75L transparent plastic pail is 2000000/ml algae solution aeration, is subsequently adding 500000 halogen
Worm naupliuss, add the culture medium of algae according to the amount of 50L algae, the amount according to 25L algae adds the culture medium of algae within the 3rd day simultaneously,
Also culture medium is added to be cultivated according to 25L algae within 6th day.Add the Pseudomonas aeruginosa of 125ml every night, algae solution is logical
Cross tap water allotment salt allotment form, its salinity be 30g dissolving salt/L, pH be 8.5 to 9.6 between fluctuate, dissolved oxygen dense
Degree is more than 6mg/L, and water temperature is 26 ± 2 DEG C；
Described algae is hailian seaweed；And use Bohai Sea Gulf artemia cysts.
The configuration process of algae solution is to add f/2 culture medium in antiseptic sea water, then inoculate algae kind in superclean bench
To in the antiseptic sea water added with f/2 culture medium, it is placed on culture in incubator；When the density of algae is 2000000/ml, by algae
Liquid moves on to culturing jar, is simultaneously introduced gas stone and carries out aeration, the salinity of antiseptic sea water is 30 ‰；Described antiseptic sea water can be by sea
Water forms using it is also possible to be allocated with tap water allotment salt after carrying out sterilization treatment.
After one week, artemia survival rate rate reaches 90%.The body length of artemia averagely increased 2.7mm.
In culturing jar, after aeration, dissolved oxygen, in 6.65mg/L, then gradually rises, and dissolved oxygen can reach within the 4th day
9.1mg/L.
The foregoing is only the preferred embodiment of the invention, not in order to limit the invention, all this
Within the spirit of innovation and creation and principle, any modification, equivalent substitution and improvement made etc., should be included in the invention
Protection domain within.
Claims (7)
1. under a breeding algal rim border artemia high-density breeding method it is characterised in that：In the water environment of artemia culture, algae is close
Spend for 1,000,000 cell/mL～10,000,000 cell/mL, and water environment includes Pseudomonas aeruginosa, concentration is 106～108
Individual/mL, the density of artemia is 5～12/mL；Preferably, the density of artemia is 5～10/mL.
2. under foster algal rim border according to claim 1 artemia high-density breeding method it is characterised in that：The salt of water environment
Spend for 25～35g dissolving salt/L, pH is 8 to 10, the concentration of dissolved oxygen is more than 6mg/L, and water temperature is 22～30 DEG C.
3. under foster algal rim border according to claim 1 artemia high-density breeding method it is characterised in that：Artemia culture
In water environment, the density of artemia is 10/mL.
4. under foster algal rim border according to claim 1 artemia high-density breeding method it is characterised in that：Described water environment
In added with algae nutritive salt, water environment is that the nutritive salt of antiseptic sea water and algae configures and forms.
5. under foster algal rim border according to claim 4 artemia high-density breeding method it is characterised in that：In every liter of sea water
The nutritive salt of algae include following component, sodium nitrate 75mg, sodium dihydrogen phosphate 5mg, sodium silicate 30mg, zinc sulfate 0.023mg, sulfur
Sour copper 0.01mg, manganese chloride 0.012mg, iron chloride 3.16mg, sodium molybdate 0.07mg, b diammonium edta sodium 4.36mg, chlorination
Cobalt 0.012mg, vitamin B120.5mg, biotin 0.5mg, vitamin B10.1mg.
6. under foster algal rim border according to claim 1 artemia high-density breeding method it is characterised in that：Described algae be etc.
Whip chrysophyceae or hailian seaweed；Described artemia is Bohai Sea Gulf artemia.
7. under the foster algal rim border according to any one of claim 1～6 artemia high-density breeding method it is characterised in that：
Comprise the steps：
1), the configuration of algae solution：Algae kind is inoculated in the antiseptic sea water added with f/2 culture medium in superclean bench, is placed on culture
Cultivate in case；Preferably, the salinity of antiseptic sea water is 30 ‰；
2), treat 1,000,000 cell/mL～10,000,000 cell/mL of density of algae, algae solution is moved on to culturing jar, is simultaneously introduced gas
Stone carries out aeration；
3), the hatching of artemia, artemia cysts are poured in hatching pipe, are subsequently adding the sea water that salinity is 20 ‰, and aeration 20～26 is little
Shi Jinhang is hatched；Preferably aeration 24 hours；
4), the cultivation of artemia
A) artemia is put into after algae solution, the meltage in measurement water is so as to be not less than 6mg/L；
B) add Pseudomonas aeruginosa, then added once at interval of 24 hours；
C) interval adds an algae media in two days, adds for the first time the culture medium of amount completely, second and third time according to
Completely the culture medium of amount 1/2 addition.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754402A (en) * | 2019-12-09 | 2020-02-07 | 海南晨海水产有限公司 | Indoor high-density cultivation method for artemia |
| CN112293606A (en) * | 2020-10-12 | 2021-02-02 | 上海海洋大学 | Method for preparing high-value aquatic bait by using microalgae and artemia |
| CN113557998A (en) * | 2021-09-27 | 2021-10-29 | 渤海水产科技(滨州)有限公司 | Ecological breeding method for high-quality shrimps |
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| CN103478081A (en) * | 2013-08-26 | 2014-01-01 | 李坚 | Indoor high-density cultivation method of artemia |
| CN104186431A (en) * | 2014-08-20 | 2014-12-10 | 天津海友佳音生物科技股份有限公司 | High-density artemia breeding method with single-cell protein single-step food chain utilized |
| CN105766810A (en) * | 2016-04-06 | 2016-07-20 | 鲁东大学 | Method for indoor low-cost high-density sustainable cultivation of artemia |
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2016
- 2016-08-31 CN CN201610784048.5A patent/CN106386704B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478081A (en) * | 2013-08-26 | 2014-01-01 | 李坚 | Indoor high-density cultivation method of artemia |
| CN104186431A (en) * | 2014-08-20 | 2014-12-10 | 天津海友佳音生物科技股份有限公司 | High-density artemia breeding method with single-cell protein single-step food chain utilized |
| CN105766810A (en) * | 2016-04-06 | 2016-07-20 | 鲁东大学 | Method for indoor low-cost high-density sustainable cultivation of artemia |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754402A (en) * | 2019-12-09 | 2020-02-07 | 海南晨海水产有限公司 | Indoor high-density cultivation method for artemia |
| CN110754402B (en) * | 2019-12-09 | 2021-10-26 | 海南晨海水产有限公司 | Indoor high-density cultivation method for artemia |
| CN112293606A (en) * | 2020-10-12 | 2021-02-02 | 上海海洋大学 | Method for preparing high-value aquatic bait by using microalgae and artemia |
| CN113557998A (en) * | 2021-09-27 | 2021-10-29 | 渤海水产科技(滨州)有限公司 | Ecological breeding method for high-quality shrimps |
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| CN106386704B (en) | 2019-10-29 |
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Effective date of registration: 20171110 Address after: Eight in the Taiwan Industrial Park Tianjin city Jinnan District Tianhua road 300222 No. 5 Applicant after: TIANJIN OCEAN PAL CAROL BIOTECH Co.,Ltd. Applicant after: TIANJIN AGRICULTURAL University Address before: Eight in the Taiwan Industrial Park Tianjin city Jinnan District Tianhua road 300222 No. 5 Applicant before: TIANJIN OCEAN PAL CAROL BIOTECH Co.,Ltd. |
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