CN106386704B - The method of artemia high-density breeding under one breeding algal rim border - Google Patents
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- CN106386704B CN106386704B CN201610784048.5A CN201610784048A CN106386704B CN 106386704 B CN106386704 B CN 106386704B CN 201610784048 A CN201610784048 A CN 201610784048A CN 106386704 B CN106386704 B CN 106386704B
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- 241001247197 Cephalocarida Species 0.000 title claims abstract description 66
- 238000009395 breeding Methods 0.000 title claims abstract description 25
- 230000001488 breeding effect Effects 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 17
- 241000195493 Cryptophyta Species 0.000 claims abstract description 60
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 8
- 239000001963 growth medium Substances 0.000 claims description 22
- 239000013535 sea water Substances 0.000 claims description 22
- 230000002421 anti-septic effect Effects 0.000 claims description 18
- 150000003839 salts Chemical class 0.000 claims description 10
- 238000005273 aeration Methods 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 9
- 235000013601 eggs Nutrition 0.000 claims description 5
- 230000012447 hatching Effects 0.000 claims description 5
- 230000000050 nutritive effect Effects 0.000 claims description 5
- 239000004575 stone Substances 0.000 claims description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 4
- 241001474374 Blennius Species 0.000 claims description 4
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 4
- 241001501873 Isochrysis galbana Species 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 2
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 2
- 229960002685 biotin Drugs 0.000 claims description 2
- 235000020958 biotin Nutrition 0.000 claims description 2
- 239000011616 biotin Substances 0.000 claims description 2
- KYQODXQIAJFKPH-UHFFFAOYSA-N diazanium;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [NH4+].[NH4+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O KYQODXQIAJFKPH-UHFFFAOYSA-N 0.000 claims description 2
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 claims description 2
- 235000002867 manganese chloride Nutrition 0.000 claims description 2
- 239000011565 manganese chloride Substances 0.000 claims description 2
- 229940099607 manganese chloride Drugs 0.000 claims description 2
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 2
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 2
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 2
- 235000015393 sodium molybdate Nutrition 0.000 claims description 2
- 239000011684 sodium molybdate Substances 0.000 claims description 2
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 2
- 235000010344 sodium nitrate Nutrition 0.000 claims description 2
- 239000004317 sodium nitrate Substances 0.000 claims description 2
- 239000011691 vitamin B1 Substances 0.000 claims description 2
- 239000011715 vitamin B12 Substances 0.000 claims description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 2
- 229960001763 zinc sulfate Drugs 0.000 claims description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 2
- 238000007792 addition Methods 0.000 claims 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 1
- 239000004115 Sodium Silicate Substances 0.000 claims 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims 1
- 239000005864 Sulphur Substances 0.000 claims 1
- 238000005660 chlorination reaction Methods 0.000 claims 1
- 229910017052 cobalt Inorganic materials 0.000 claims 1
- 239000010941 cobalt Substances 0.000 claims 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims 1
- 229910052802 copper Inorganic materials 0.000 claims 1
- 239000010949 copper Substances 0.000 claims 1
- 235000019795 sodium metasilicate Nutrition 0.000 claims 1
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims 1
- 229910052911 sodium silicate Inorganic materials 0.000 claims 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 14
- 239000001301 oxygen Substances 0.000 abstract description 14
- 229910052760 oxygen Inorganic materials 0.000 abstract description 14
- 230000004083 survival effect Effects 0.000 abstract description 13
- 230000000630 rising effect Effects 0.000 abstract description 2
- 238000004904 shortening Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 13
- 239000012267 brine Substances 0.000 description 5
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 5
- 239000008399 tap water Substances 0.000 description 5
- 235000020679 tap water Nutrition 0.000 description 5
- 241000133262 Nauplius Species 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 229910052736 halogen Inorganic materials 0.000 description 4
- 150000002367 halogens Chemical class 0.000 description 4
- 241000238582 Artemia Species 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 2
- 241000143060 Americamysis bahia Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000251511 Holothuroidea Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000001320 hippocampus Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000034004 oogenesis Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides the method for artemia high-density breeding under a breeding algal rim border, the density of algae is 1,000,000 cell/mL~10,000,000 cell/mL in the water environment of artemia culture, and includes pseudomonas aeruginosa, concentration 10 in water environment6~108A/mL, the density of artemia are 5~12/mL；Preferably, the density of artemia is 5~10/mL.This method not only realizes the rising of the dissolved oxygen in water, solves the problems, such as artemia open-mouthed bait, and the problem that the spawning rate for shortening the survival rate sum of high-density breeding simultaneously is low, the present invention is also suitble to guarantee stable water environment and survival rate in large-scale cultivating pool.
Description
Technical field
The invention belongs to high-density breeding artemia technical fields, support more particularly, to artemia high density under a breeding algal rim border
The method grown.
Background technique
Artemia " shrimps " the big artemia that is otherwise known as is generally the preferred bait of shrimp, crab, hippocampus, sea cucumber.It is general in the market
There is no life food as big artemia, only artemia freezes body, and the nauplius hatched became big by of short duration one week
Artemia not only solves the problems, such as that artemia high density survival rate is low and culturing time is long, also solves enterprise's purchase and freezes big halogen
The economic cost and the big stale problem of artemia of frost of worm.But currently, people are to bait needed for artemia high-density breeding
It has different opinions, therefore in terms of artemia high-density breeding, not yet it has been proposed that influence of the different bait to artemia high-density breeding,
By upgrowth situation of the artemia in different algaes, so that the cultural method of artemia rapidly and efficiently is found, thus save the cost.
Summary of the invention
In view of this, the present invention is directed to propose under a breeding algal rim border artemia high-density breeding method, this method is not
But the growth for realizing algae, for artemia, oxygen demand provides guarantee under high density while carrying out photosynthesis and producing oxygen, solution
Artemia of the having determined problem slow because of bait hypotrophy, and guarantee that artemia possesses high survival rate simultaneously, the present invention is also suitble to
Big cultivation place guarantees stable survival rate.
In order to achieve the above objectives, the technical scheme of the present invention is realized as follows:
The method of artemia high-density breeding under one breeding algal rim border, the density of algae is 1,000,000 in the water environment of artemia culture
A cell/mL~10,000,000 cell/mL, and include pseudomonas aeruginosa, concentration 10 in water environment6~108A/mL, artemia
Density be 5~12/mL；Preferably, the density of artemia is 5~10/mL.
Preferably, the salinity of water environment is 25~35g dissolving salt/L, and pH is 8 to 10, and the concentration of dissolved oxygen is greater than 6mg/L,
Water temperature is 22~30 DEG C.
Preferably, the density of artemia is 10/mL in the water environment of artemia culture.
Preferably, in the water environment added with the nutritive salt of algae, water environment be the nutritive salt configuration of antiseptic sea water and algae and
At.
Preferably, the nutritive salt of the algae in every liter of seawater includes following component, sodium nitrate 75mg, sodium dihydrogen phosphate 5mg, silicon
Sour sodium 30mg, zinc sulfate 0.023mg, copper sulphate 0.01mg, manganese chloride 0.012mg, iron chloride 3.16mg, sodium molybdate 0.07mg,
B diammonium edta sodium 4.36mg, cobalt chloride 0.012mg, vitamin B120.5mg, biotin 0.5mg, vitamin B10.1mg。
Preferably, the algae is Isochrysis galbana or hailian seaweed；The artemia is Bohai Sea Gulf artemia.
Preferably, include the following steps:
1) it, the configuration of algae solution: by algae in the antiseptic sea water that superclean bench is inoculated into added with f/2 culture medium, is placed on
It is cultivated in incubator；Preferably, the salinity of antiseptic sea water is 30 ‰；；
2), to 1,000,000 cell/mL~10,000,000 cell/mL of the density of algae, algae solution is moved on into culturing jar, is added simultaneously
Enter gas stone to be aerated；
3), the hatching of artemia, artemia eggs is poured into hatching pipe, the seawater that then addition salinity is 20 ‰, and aeration 20~
Hatched within 26 hours；Preferred aeration 24 hours；
4), the cultivation of artemia
A) after artemia being put into algae solution, the meltage in water is measured, makes it not less than 6mg/L；
B) pseudomonas aeruginosa is added, it is then primary at interval of addition in 24 hours；
C) it is spaced the algae media of addition in two days, the culture medium measured completely is added for the first time, second and third time
It is added according to the 1/2 of the culture medium measured completely.
Compared with the existing technology, under breeding algal rim border of the present invention artemia high-density breeding method, have with
Lower advantage:
1) present invention is realized in algae solution middle-high density artemia culture ovum, is guaranteed that artemia possesses high survival rate, is counted after 7 days
The survival rate for calculating artemia reaches 89% or more, and survival rate is lower during solving the problems, such as artemia high-density breeding.
2) the method for the present invention not only realizes the rising of the dissolved oxygen in water, solves the problems, such as artemia open-mouthed bait, and simultaneously
The low problem of the spawning rate of the survival rate sum of high-density breeding is shortened, the present invention is also suitble to guarantee to stablize in large-scale cultivating pool
Water environment and survival rate.
3) under suitable condition, promote artemia in the mating of appearance in one week and oogenesis phenomenon, solve the artemia speed of growth
Slower problem.
Specific embodiment
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.
Below in conjunction with embodiment, the present invention will be described in detail.
Embodiment one
10L density is added as 2000000/ml algae solution aeration using 20L transparent glass cylinder, 100000 halogen are then added
Worm nauplius, while according to the culture medium that algae is added with the amount for comparing 10L algae of f/2 culture medium, third day is according to 5L algae
The culture medium of algae is added in amount, culture medium also is added according to 5L algae within the 6th day and is cultivated.Every afternoon 4 points of addition 25ml concentration
It is 109The pseudomonas aeruginosa of a/mL, addition in every 24 hours is primary, and salinity is 30g dissolving salt/L, pH be 8.5 to 9.6 it
Between fluctuate, the concentration of dissolved oxygen is greater than 6mg/L, and water temperature is 26 ± 2 DEG C；
The configuration process of algae solution is that f/2 culture medium is added in antiseptic sea water, is then inoculated with algae in superclean bench
Into the antiseptic sea water added with f/2 culture medium, puts and cultivate in the incubator；When the density of algae is 2000000/ml, by algae
Liquid pours into culturing jar, while gas stone is added and is aerated, and the salinity of antiseptic sea water is 30 ‰；The antiseptic sea water can be will be extra large
Water uses after carrying out sterilization treatment, can also deploy brine with tap water and deploy.
The algae is Isochrysis galbana；And use Bohai Sea Gulf artemia eggs.
Reach 89% by artemia survival rate rate after a week, the body length of artemia averagely increases 3.2mm.
In culturing jar, after aeration, then dissolved oxygen gradually rises in 6.65mg/L, and dissolved oxygen can reach within the 4th day
9.1mg/L。
Embodiment two
10L density is added as 2000000/ml algae solution aeration using 20L transparent glass cylinder, 100000 halogen are then added
Worm nauplius, while according to the culture medium of the amount of 10L algae addition algae, the culture medium of algae is added according to the amount of 5L algae for third day,
Also culture medium is added according to 5L algae within 6th day to be cultivated.The pseudomonas aeruginosa of 25ml is added in every night, and algae solution is to pass through
Tap water allotment brine is deployed, and salinity is 30g dissolving salt/L, and pH is to fluctuate between 8.5 to 9.6, the concentration of dissolved oxygen
Greater than 6mg/L, water temperature is 26 ± 2 DEG C；
The configuration process of algae solution is that f/2 culture medium is added in antiseptic sea water, is then inoculated with algae in superclean bench
Into the antiseptic sea water added with f/2 culture medium, puts and cultivate in the incubator；When the density of algae is 2000000/ml, by algae
Liquid pours into culturing jar, while gas stone is added and is aerated, and the salinity of antiseptic sea water is 30 ‰；The antiseptic sea water can be will be extra large
Water uses after carrying out sterilization treatment, can also deploy brine with tap water and deploy.
The algae is hailian seaweed；And use Bohai Sea Gulf artemia eggs.
Reach 94% by artemia survival rate rate after a week, the body length of artemia averagely increases 2.5mm.
In culturing jar, after aeration, then dissolved oxygen gradually rises in 6.75mg/L, and dissolved oxygen can reach within the 4th day
9.3mg/L。
Embodiment three
50L density is added as 2000000/ml algae solution aeration using 75L transparent plastic pail, 500000 halogen are then added
Worm nauplius, while according to the culture medium of the amount of 50L algae addition algae, the culture medium of algae is added according to the amount of 25L algae for third day,
Also culture medium is added according to 25L algae within 6th day to be cultivated.The pseudomonas aeruginosa of 125ml is added in every night, and algae solution is logical
Cross tap water allotment brine deploy, salinity be 30g dissolving salt/L, pH be 8.5 to 9.6 between fluctuate, dissolved oxygen it is dense
Degree is greater than 6mg/L, and water temperature is 26 ± 2 DEG C；
The algae is hailian seaweed；And use Bohai Sea Gulf artemia eggs.
The configuration process of algae solution is that f/2 culture medium is added in antiseptic sea water, is then inoculated with algae in superclean bench
Into the antiseptic sea water added with f/2 culture medium, puts and cultivate in the incubator；When the density of algae is 2000000/ml, by algae
Liquid moves on to culturing jar, while gas stone is added and is aerated, and the salinity of antiseptic sea water is 30 ‰；The antiseptic sea water can be will be extra large
Water uses after carrying out sterilization treatment, can also deploy brine with tap water and deploy.
Reach 90% by artemia survival rate rate after a week.The body length of artemia averagely increases 2.7mm.
In culturing jar, after aeration, then dissolved oxygen gradually rises in 6.65mg/L, and dissolved oxygen can reach within the 4th day
9.1mg/L。
The foregoing is merely the preferred embodiments of the invention, are not intended to limit the invention creation, all at this
Within the spirit and principle of innovation and creation, any modification, equivalent replacement, improvement and so on should be included in the invention
Protection scope within.
Claims (4)
1. the method for artemia high-density breeding under a breeding algal rim border, characterized by the following steps:
1), the configuration of algae solution: by algae in the antiseptic sea water that superclean bench is inoculated into added with f/2 culture medium, it is placed on culture
It is cultivated in case；
2), to 1,000,000 cell/mL~10,000,000 cell/mL of the density of algae, algae solution is moved on into culturing jar, while gas is added
Stone is aerated；
3), the hatching of artemia, artemia eggs is poured into hatching pipe, and the seawater that salinity is 20 ‰ is then added, and aeration 20~26 is small
Shi Jinhang hatching；
4), the cultivation of artemia；
A) after artemia being put into algae solution, the meltage in water is measured, makes it not less than 6mg/L；
B) pseudomonas aeruginosa is added, it is then primary at interval of addition in 24 hours；
C) be spaced the algae media of additions in two days, the culture medium measured completely is added for the first time, for the second time with for the third time according to
The 1/2 of the culture medium measured completely is added；
The density of algae is 1,000,000 cell/mL~10,000,000 cell/mL in the water environment of artemia culture, and is wrapped in water environment
Include pseudomonas aeruginosa, concentration 106~108A/mL, the density of artemia are 5~12/mL；The salinity of water environment be 25~
35g dissolving salt/L, pH are 8 to 10, and water temperature is 22~30 DEG C；The water environment be the nutritive salt configuration of antiseptic sea water and algae and
At；The algae is Isochrysis galbana or hailian seaweed；The artemia is Bohai Sea Gulf artemia.
2. the method for artemia high-density breeding under feeding algal rim border according to claim 1, it is characterised in that: antiseptic sea water
Salinity is 30 ‰, and the density of artemia is 5~10/mL.
3. the method for artemia high-density breeding under feeding algal rim border according to claim 1, it is characterised in that: artemia culture
The density of artemia is 10/mL in water environment.
4. the method for artemia high-density breeding under feeding algal rim border according to claim 1, it is characterised in that: in every liter of seawater
The nutritive salt of algae include following component, sodium nitrate 75mg, sodium dihydrogen phosphate 5mg, sodium metasilicate 30mg, zinc sulfate 0.023mg, sulphur
Sour copper 0.01mg, manganese chloride 0.012mg, iron chloride 3.16mg, sodium molybdate 0.07mg, b diammonium edta sodium 4.36mg, chlorination
Cobalt 0.012mg, vitamin B120.5mg, biotin 0.5mg, vitamin B10.1mg。
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| CN110754402B (en) * | 2019-12-09 | 2021-10-26 | 海南晨海水产有限公司 | Indoor high-density cultivation method for artemia |
| CN112293606A (en) * | 2020-10-12 | 2021-02-02 | 上海海洋大学 | Method for preparing high-value aquatic bait by using microalgae and artemia |
| CN113557998B (en) * | 2021-09-27 | 2022-01-04 | 渤海水产科技(滨州)有限公司 | Ecological breeding method for high-quality shrimps |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478081A (en) * | 2013-08-26 | 2014-01-01 | 李坚 | Indoor high-density cultivation method of artemia |
| CN104186431A (en) * | 2014-08-20 | 2014-12-10 | 天津海友佳音生物科技股份有限公司 | High-density artemia breeding method with single-cell protein single-step food chain utilized |
| CN105766810A (en) * | 2016-04-06 | 2016-07-20 | 鲁东大学 | Method for indoor low-cost high-density sustainable cultivation of artemia |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478081A (en) * | 2013-08-26 | 2014-01-01 | 李坚 | Indoor high-density cultivation method of artemia |
| CN104186431A (en) * | 2014-08-20 | 2014-12-10 | 天津海友佳音生物科技股份有限公司 | High-density artemia breeding method with single-cell protein single-step food chain utilized |
| CN105766810A (en) * | 2016-04-06 | 2016-07-20 | 鲁东大学 | Method for indoor low-cost high-density sustainable cultivation of artemia |
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Effective date of registration: 20171110 Address after: Eight in the Taiwan Industrial Park Tianjin city Jinnan District Tianhua road 300222 No. 5 Applicant after: TIANJIN OCEAN PAL CAROL BIOTECH Co.,Ltd. Applicant after: TIANJIN AGRICULTURAL University Address before: Eight in the Taiwan Industrial Park Tianjin city Jinnan District Tianhua road 300222 No. 5 Applicant before: TIANJIN OCEAN PAL CAROL BIOTECH Co.,Ltd. |
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