CN106386500B - A kind of culture medium of garlic tissue cultural seedlings of free - Google Patents
A kind of culture medium of garlic tissue cultural seedlings of free Download PDFInfo
- Publication number
- CN106386500B CN106386500B CN201610952916.6A CN201610952916A CN106386500B CN 106386500 B CN106386500 B CN 106386500B CN 201610952916 A CN201610952916 A CN 201610952916A CN 106386500 B CN106386500 B CN 106386500B
- Authority
- CN
- China
- Prior art keywords
- culture medium
- garlic
- medium
- tissue
- free
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The present invention relates to tissue culture medium (TCM) technical fields, and in particular to a kind of culture medium of garlic tissue cultural seedlings of free.Culture medium of the present invention is basic culture medium with MS culture medium or B5 medium, in terms of basal medium, further include following quality-volumetric concentration active constituent: 6-BA 0.5-2.0mg/L, NAA 0.05-0.5mg/L, brassin lactones 0.01-0.1mg/L, jasmonic 0.02-0.08mg/L, phloridzin 0.1-0.8mg/L, active carbon 1-3g/L, potato pulp 30-50g/L, multi-vitamins 0.2-0.4mg/L.Culture medium pH value is 5.6-6.0.The present invention provides a kind of culture medium of garlic detoxification garlic tissue cultural seedlings of free, effectively facilitates the division of garlic in vitro tissue and growth, improves transplanting motility rate and bud ratio, is suitble to use in garlic detoxification tissue cultures, is particularly suitable for Yulin garlic and uses in tissue cultures.
Description
[technical field]
The present invention relates to tissue culture medium (TCM) technical fields, and in particular to a kind of culture medium of garlic tissue cultural seedlings of free.
[background technique]
Yulin garlic has had more than 1,000 years cultivation histories.Its head is big, grain valve is solid, pungent succulence, the crisp taste perfume of meat,
Stem skin color is mostly purple, also there is white.It is listed in Guangxi native article, market is wide, and export value is high, good in economic efficiency.It produces
Ground concentrates on the ground such as Ren Dong, honest and kindhearted, good fortune is continuous, the big Pingshan Mountain.
Garlic smell pungent-warm, the nutriments such as protein rich in, fat, sugar, calcium, Iron and Vitamin A, B, C, and
Medical value with higher has the effect of " anti-inflammatory, reason stomach, middle benefit gas, evil removing numbness poison gas ".Garlic or common condiment,
When boiling melon, dish, fish, meat, several valves are put into, fragrance is palatable.
Yulin garlic is the famous-brand and high-quality local characteristic agricultural product in Guangxi, because of it with quality uniqueness, aromatic flavour, pungent it is strong,
The advantages that garlic oil is abundant, clothing pool is purplish red, it is well received on the market.It is detected according to relevant department, Yulin garlic is that current China is big
Fragrance is most dense in garlic kind, pungent is most strong, the most abundant kind of garlic oil, is extremely suitable for processing garlic oil, smashed garlic it is also known as Yulin perfume (or spice) garlic
And seasoned food.
Garlic is asexually propagated plant, and since garlic not can be carried out sexual propagation, usually peasant planting family is reserved for one's own use throughout the year
Kind garlic, limited natural variation are not able to satisfy the requirement of genetic improvement, are difficult Accurate Determining secondary generation by traditional method
Thank constituent content and evaluation hereditary variation.Yulin garlic breeds by bulb, accumulates virus year by year by garlic kind and phase from generation to generation
It passes, Different Varieties is caused seriously to be degenerated, seriously constrain the large-scale production of Yulin garlic and sustainable and healthy development, limiting will be beautiful
Woods garlic forges into the paces of regional characteristic product.
At present seldom about Yulin garlic is directed to, the research that tissue cultures are bred is carried out using Allium fistulosum stem tips, although
Other related kinds carry out the report of tissue cultures at present, but since Yulin garlic cultivar and other kinds are there are certain difference,
Tissue culture effect is poor.Lack the cultural method and special culture media of a kind of tissue cultural seedlings of free specifically for Yulin garlic.
The information disclosed in the background technology section is intended only to increase the understanding to general background of the invention, without answering
When being considered as recognizing or imply that the information constitutes the prior art already known to those of ordinary skill in the art in any form.
[summary of the invention]
Goal of the invention of the invention is: being directed to the problems of the prior art, provides a kind of garlic detoxification garlic detoxification group
The culture medium for training seedling effectively facilitates the division of garlic in vitro tissue and growth, improves transplanting motility rate and bud ratio, is suitble to garlic detoxification
It is used in tissue cultures, is particularly suitable for Yulin garlic and is used in tissue cultures.
To achieve the goals above, The technical solution adopted by the invention is as follows:
A kind of culture medium of garlic tissue cultural seedlings of free, culture medium are basic culture medium with MS culture medium or B5 medium, with
Basal medium meter, further include following quality-volumetric concentration active constituent: 6-BA 0.5-2.0mg/L, NAA 0.05-
0.5mg/L, the culture medium pH value are 5.6-6.0.
Optimization, the basal medium is selected from B5 medium.
It is more optimized, in terms of basal medium, the 6-BA be 1.2-1.5mg/L, the NAA be 0.3-0.5mg/L.
It advanced optimizes, further includes the active constituent of following quality-volumetric concentration: brassin lactones in culture medium
0.01-0.1mg/L, jasmonic 0.02-0.08mg/L, phloridzin 0.1-0.8mg/L, active carbon 1-3g/L, potato pulp 30-
50g/L, multi-vitamins 0.2-0.4mg/L.
Further optimize, the active constituent quality-volumetric concentration are as follows: brassin lactones 0.03-0.08mg/L, jasmine
Jasmine acid 0.04-0.06mg/L, phloridzin 0.3-0.6mg/L, active carbon 1.5-2.5g/L, potato pulp 35-45g/L, compound dimension
Raw element 0.25-0.35mg/L.
Further optimize again, the active constituent is in terms of basal medium, and Components Name and quality-volumetric concentration are such as
Under: 6-BA 1.4mg/L, NAA 0.4mg/L, brassin lactones 0.05mg/L, jasmonic 0.05mg/L, phloridzin 0.4mg/L,
Active carbon 2g/L, potato pulp 30g/L, multi-vitamins 0.3mg/L.
Further optimize again, the culture medium pH value is 5.8.
In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:
1. being added to brassin lactones in culture medium of the present invention, has dosage few, can inhibit the activity of auxin oxidase,
The content of plant endogenous auxin is improved, to remarkably promote the elongation growth of garlic.Life in brassin lactones and culture medium
Long element such as NAA and other active components such as jasmonic, activity have facilitation between each other, collectively promote in Garlic Tissue
The growth of tissue or tissue-cultured seedling.Division, the differentiation of Garlic Tissue cell can be also effectively facilitated in the present invention added with jasmonic,
Cooperatively promote Garlic Tissue cell division with 6-BA.It can significantly promote garlic root after addition jasmonic to become mostly and grow,
The expression that defence plant gene can be excited, induces the chemical defence of plant, adapts to garlic tissue-cultured seedling in planting process from now on
Ability enhancing, transplant survival are high.
2. adding certain density active carbon in culture medium of the present invention, Garlic Tissue can be effectively reduced and culture medium becomes
Color effectively adsorbs impurity in harmful substance and the culture medium in culture medium, produces to the detrimental metabolic generated in tissue culture procedures
Object can also play the role of absorption, the survival rate during reducing garlic tissue culture from face.Phloridzin in the present invention can be
It under culture medium acidic environment, can effectively inhibit harmful microbe activity in culture medium, extend culture medium and go bad, with activity
Charcoal collective effect, it is effective that culture medium is kept to stablize, under the ambient condition in suitable Garlic Growth.Culture medium and base of the present invention
Basal culture medium is compared, and Coloring Time can postpone culture medium 5-8 days.
3. the present invention select MS culture medium or B5 medium for basis culture medium, it can have maintenance and leave one's post plant cell base
Major part nutritional ingredient required for this life as the culture medium during garlic tissue culture is inadequate with this.The present invention is logical
Cross the various active constituents for adding and being suitble to Garlic Growth that there is facilitation, supplement in basal nutrient nutrition needed for garlic tissue culture at
Point, it is balanced nutritious；Using each Substance Interactions, suitable special culture media is provided for the tissue culture procedures of garlic.This
The garlic special culture media provided is provided, it is full of nutrition, it is prepared for garlic plant characteristic, garlic budding stem can be effectively improved
Sharp number and bud induction rate, tissue-cultured seedling well developed root system, stem thickness is strong, and vitality is indomitable.
[specific embodiment]
Below with reference to embodiment, the invention will be further described.
Embodiment 1
A kind of culture medium of garlic tissue cultural seedlings of free, culture medium is basic culture medium with MS culture medium, with basal medium
Count, further include following quality-volumetric concentration active constituent: 6-BA 0.5mg/L, NAA 0.05mg/L, the culture medium pH value
It is 5.6.
Embodiment 2
A kind of culture medium of garlic tissue cultural seedlings of free, culture medium is basic culture medium with MS culture medium, with basal medium
Count, further include following quality-volumetric concentration active constituent: 6-BA 2.0mg/L, NAA 0.5mg/L, culture medium pH value are
6.0。
Embodiment 3
A kind of culture medium of garlic tissue cultural seedlings of free, culture medium is basic culture medium with MS culture medium, with basal medium
Count, further include following quality-volumetric concentration active constituent: 6-BA 1.5mg/L, NAA 0.3mg/L, culture medium pH value are
5.8。
Embodiment 4
A kind of culture medium of garlic tissue cultural seedlings of free, culture medium is basic culture medium with B5 medium, with basal medium
Count, further include following quality-volumetric concentration active constituent: 6-BA 1.5mg/L, NAA 0.3mg/L, culture medium pH value are
5.8。
Embodiment 5
A kind of culture medium of garlic tissue cultural seedlings of free, culture medium is basic culture medium with B5 medium, with basal medium
Count, further include following quality-volumetric concentration active constituent: 6-BA 1.2mg/L, NAA 0.1mg/L, brassin lactones
0.01mg/L, jasmonic 0.02mg/L, phloridzin 0.1mg/L, active carbon 1g/L, potato pulp 30g/L, multi-vitamins
0.2mg/L, culture medium pH value are 5.7.
Embodiment 6
A kind of culture medium of garlic tissue cultural seedlings of free, culture medium is basic culture medium with B5 medium, with basal medium
Count, further include following quality-volumetric concentration active constituent: 6-BA 2.0mg/L, NAA 0.5mg/L, brassin lactones
0.1mg/L, jasmonic 0.08mg/L, phloridzin 0.8mg/L, active carbon 3g/L, potato pulp 50g/L, multi-vitamins
0.4mg/L, culture medium pH value are 5.9.
Embodiment 7
A kind of culture medium of garlic tissue cultural seedlings of free, culture medium is basic culture medium with B5 medium, with basal medium
Count, further include following quality-volumetric concentration active constituent: 6-BA 1.2mg/L, NAA 0.3mg/L, brassin lactones
0.03mg/L, jasmonic 0.04mg/L, phloridzin 0.3mg/L, active carbon 1.5g/L, potato pulp 35g/L, multi-vitamins
0.25mg/L, culture medium pH value are 5.8.
Embodiment 8
A kind of culture medium of garlic tissue cultural seedlings of free, culture medium is basic culture medium with B5 medium, with basal medium
Count, further include following quality-volumetric concentration active constituent: 6-BA 1.5mg/L, NAA 0.5mg/L, brassin lactones
0.08mg/L, jasmonic 0.06mg/L, phloridzin 0.6mg/L, active carbon 2.5g/L, potato pulp 45g/L, multi-vitamins
0.35mg/L, culture medium pH value are 5.8.
Embodiment 9
A kind of culture medium of garlic tissue cultural seedlings of free, culture medium is basic culture medium with B5 medium, with basal medium
Count, further include following quality-volumetric concentration active constituent: 6-BA 1.4mg/L, NAA 0.4mg/L, brassin lactones
0.05mg/L, jasmonic 0.05mg/L, phloridzin 0.4mg/L, active carbon 2g/L, potato pulp 30g/L, multi-vitamins
0.3mg/L.Culture medium pH value is 5.8.
Piglet s colibacillosis
Laboratory sample: embodiment 1-8 is respectively as real all product 1-8.
Control medium is set simultaneously, and control medium is provided that control 1 is B5 medium；Control 2 are cultivated for MS
Base
Experimental method: laboratory sample is to take Yulin garlic using identical tissue culture operating method with a collection of Yulin garlic
Allium fistulosum stem tips are inoculated into respectively in different culture mediums, by cultivating a period of time under same condition of culture, observe different trainings
The budding number of each garlic on base is supported, bud ratio is calculated.
Different culture medium influences the bud ratio of garlic tissue-cultured seedling
| Sample | It is inoculated with stem apex number (a) | It sprouts number (a) | Bud ratio (%) |
| Embodiment 1 | 100 | 29 | 29 |
| Embodiment 2 | 100 | 34 | 34 |
| Embodiment 3 | 100 | 46 | 46 |
| Embodiment 4 | 100 | 52 | 52 |
| Embodiment 5 | 100 | 85 | 85 |
| Embodiment 6 | 100 | 84 | 84 |
| Embodiment 7 | 100 | 90 | 90 |
| Embodiment 8 | 100 | 92 | 92 |
| Embodiment 9 | 100 | 96 | 96 |
| Control 1 | 100 | 16 | 16 |
| Control 2 | 100 | 12 | 12 |
MS and two kinds of B5 different minimal mediums are respectively adopted from embodiment 3 and embodiment 4, other conditions are same, carry out
Yulin Allium fistulosum stem tips bud induction differentiation culture, the results showed that the Allium fistulosum stem tips of inoculation can induce differentiation in MS and B5 medium
Budding and seedling, and it is higher with the bud ratio of B5 medium, in contrast, the bud of MS culture medium induces slow 2-3 days, bud ratio
Low, Yulin Allium fistulosum stem tips culture is preferable using B5 minimal medium.
Embodiment 5-9 and embodiment 1-4 and compare No. 1-2 it is opposite, bud ratio is apparently higher than two next, and surface increases more
The culture medium of kind ingredient is more suitable for the growth of Yulin garlic.And with 9 highest of embodiment.
The culture medium basic relative to two compares No. 1 and control 2, is added to different activities ingredient in embodiment 1-9
Culture medium be all substantially higher in control No. 1 and control No. 2.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to
In the covered the scope of the patents of the present invention.
Claims (4)
1. a kind of culture medium of garlic tissue cultural seedlings of free, which is characterized in that culture medium is basic culture medium with B5 medium, with base
Basal culture medium meter further includes the active constituent of following quality-volumetric concentration: 6-BA 0.5-2.0mg/L, NAA 0.05-0.5mg/
L, brassin lactones 0.01-0.1mg/L, jasmonic 0.02-0.08mg/L, phloridzin 0.1-0.8mg/L, active carbon 1-3g/L,
Potato pulp 30-50g/L, multi-vitamins 0.2-0.4mg/L, the Medium's PH Value are 5.6-6.0；
The culture medium is applied to the Allium fistulosum stem tips of Yulin garlic.
2. a kind of culture medium of garlic tissue cultural seedlings of free according to claim 1, which is characterized in that in terms of basal medium,
The 6-BA is 1.2-1.5mg/L, and the NAA is 0.3-0.5mg/L.
3. a kind of culture medium of garlic tissue cultural seedlings of free according to claim 1, which is characterized in that the active constituent matter
Amount-volumetric concentration are as follows: brassin lactones 0.03-0.08mg/L, jasmonic 0.04-0.06mg/L, phloridzin 0.3-0.6mg/L,
Active carbon 1.5-2.5g/L, potato pulp 35-45g/L, multi-vitamins 0.25-0.35mg/L.
4. a kind of culture medium of garlic tissue cultural seedlings of free according to claim 1, which is characterized in that the Medium's PH Value is
5.8。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610952916.6A CN106386500B (en) | 2016-11-03 | 2016-11-03 | A kind of culture medium of garlic tissue cultural seedlings of free |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610952916.6A CN106386500B (en) | 2016-11-03 | 2016-11-03 | A kind of culture medium of garlic tissue cultural seedlings of free |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106386500A CN106386500A (en) | 2017-02-15 |
| CN106386500B true CN106386500B (en) | 2019-03-19 |
Family
ID=58014562
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610952916.6A Active CN106386500B (en) | 2016-11-03 | 2016-11-03 | A kind of culture medium of garlic tissue cultural seedlings of free |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106386500B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107232064A (en) * | 2017-07-28 | 2017-10-10 | 黄小华 | Promote the method for Momordica grosvenori CYP23 gene expressions |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR0134140B1 (en) * | 1994-10-08 | 1998-04-18 | 김희용 | Garlic tissue cultivation |
| CN1090901C (en) * | 1999-06-15 | 2002-09-18 | 南京农业大学 | Method for quickly multiplying detoxicated garlic seedlings as seed |
| CN1473463A (en) * | 2002-08-07 | 2004-02-11 | 浙江大学 | Plant tissue culture de-virusing method |
-
2016
- 2016-11-03 CN CN201610952916.6A patent/CN106386500B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN106386500A (en) | 2017-02-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Legwaila et al. | Potential of traditional food plants in rural household food security in Botswana | |
| Shrestha et al. | Diversity and traditional knowledge concerning wild food species in a locally managed forest in Nepal | |
| CN101861797B (en) | Culture method for Lyophyllum Karst liquid spawn | |
| CN107535724B (en) | Flammulina velutipes foot ferment and application thereof | |
| CN105580735A (en) | Culture solution capable of improving content of resveratrol in polygonum cuspidatum callus and culture method | |
| CN104542290A (en) | Long-time succeeding preservation method for peach callus tissues | |
| Zhang et al. | The current progress in strawberry breeding in China | |
| CN106386500B (en) | A kind of culture medium of garlic tissue cultural seedlings of free | |
| CN103598097B (en) | The cultural method of virus-free snake gourd plantlet in vitro | |
| Nabieva | Asymbiotic seed germination and in vitro seedling development of Orchis militaris, an endangered orchid in Siberia | |
| Waweru et al. | Current status of utilization and potential of Dovyalis caffra fruit: major focus on Kenya-a review | |
| CN112293615A (en) | Overwintering feed for golden pomfret and preparation and culture method thereof | |
| CN106900543A (en) | A kind of selection of the disease resisting rice kind of many nutrition polymerizations | |
| Wang et al. | Exploration of Actinidia genetic resources and development of kiwifruit industry in China | |
| CN106508676B (en) | A kind of purification and rejuvenation method of Yulin perfume (or spice) garlic | |
| KR101121447B1 (en) | In vitro media components for fast growth of some oriental lily bulblets | |
| Hiwilepo-van Hal | Processing of marula (Sclerocarya birrea subsp. Caffra) fruits: A case study on health-promoting compounds in marula pulp | |
| CN106912375A (en) | A kind of hybrid rice breeding method | |
| CN106256805A (en) | A kind of purple cuckoo tea growing nursery and culture substrate and preparation method thereof | |
| CN1312276C (en) | Method for in vitro culturing dandelion root and its special differentiation culture medium | |
| Pamo et al. | Evaluation of the comparative growth and reproductive performance of West African Dwarf Goat in the Western Highland of Cameroon | |
| CN115380871B (en) | Cultivation method of golden-tomb green-shell laying hen | |
| CN102524083B (en) | Polyploid induction method of airing-apricot tree | |
| KR102612026B1 (en) | Disease-Resistant P. tenera Variants | |
| Sakpere et al. | Investigation of nutritional composition and vegetative propagation of two leafy vegetables |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |