CN106508676B - A kind of purification and rejuvenation method of Yulin perfume (or spice) garlic - Google Patents
A kind of purification and rejuvenation method of Yulin perfume (or spice) garlic Download PDFInfo
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- CN106508676B CN106508676B CN201610953190.8A CN201610953190A CN106508676B CN 106508676 B CN106508676 B CN 106508676B CN 201610953190 A CN201610953190 A CN 201610953190A CN 106508676 B CN106508676 B CN 106508676B
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- garlic
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- perfume
- aerosol index
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The present invention relates to tissue cultures and reproduction technique field, and in particular to a kind of purification and rejuvenation method of Yulin perfume (or spice) garlic.A kind of purification and rejuvenation method of Yulin perfume (or spice) garlic of the present invention, includes the following steps：A. Yulin perfume (or spice) garlic cultivates aerosol index；B. aerosol index through handle diameter be 1~3 phyllopodium of 0.1-1.5mm and band aerosol index point；C. aerosol index point inoculated and cultured obtains unrooted tissue culture bud；D. unrooted tissue culture bud moves into proliferated culture medium and is further trained garlic tissue cultural seedlings of free.The present invention provides a kind of purification and rejuvenation method of Yulin perfume (or spice) garlic, be suitble to Yulin perfume (or spice) garlic using aerosol index point carry out tissue culture propagating, the method for the present invention have aerosol index point sprout morning, growing way soon, survival rate highest；The Yulin perfume (or spice) garlic bolt stem thickness that tissue culture comes out is strong, and vitality is indomitable, adaptable feature.
Description
【Technical field】
The present invention relates to tissue cultures and reproduction technique field, and in particular to a kind of purification and rejuvenation method of Yulin perfume (or spice) garlic.
【Background technology】
Yulin perfume (or spice) garlic has had more than 1,000 years cultivation history.Its head is big, grain valve is solid, pungent succulence, meat are crisp savory,
Stem skin color is mostly purple, also there is white.It is listed in Guangxi native article, market is wide, and export value is high, good in economic efficiency.Production
Ground concentrates on the ground such as Ren Dong, honest and kindhearted, good fortune is continuous, the big Pingshan Mountain.
Garlic smell pungent-warm, containing the nutriments such as abundant protein, fat, sugar, calcium, Iron and Vitamin A, B, C, and
With higher medical value, there is the effect of " anti-inflammatory, reason stomach, middle benefit gas, evil removing numbness poison gas ".Garlic or common condiment,
When boiling melon, dish, fish, meat, several valves are put into, fragrance is palatable.
Yulin perfume (or spice) garlic is the famous-brand and high-quality local characteristic agricultural product in Guangxi, because of it with quality uniqueness, aromatic flavour, pungent it is strong,
The advantages that garlic oil is abundant, clothing pool is purplish red, it is well received on the market.It is detected according to relevant department, Yulin perfume (or spice) garlic is that current China is big
The kind also known as Yulin garlic that fragrance is most dense in garlic kind, pungent is most strong, garlic oil is most abundant, extremely suitable for processing garlic oil, smashed garlic and
Seasoned food.
Garlic is asexually propagated plant, and since garlic cannot carry out generative propagation, typically peasant planting family is reserved for one's own use throughout the year
Kind garlic, limited natural variation can not meet the requirement of genetic improvement, be difficult Accurate Determining secondary generation by traditional method
Thank constituent content and evaluation hereditary variation.Yulin perfume (or spice) garlic is bred by bulb, and virus is made to be accumulated year by year by garlic kind and phase from generation to generation
It passes, Different Varieties is caused seriously to be degenerated, seriously constrain the large-scale production of Yulin perfume (or spice) garlic and sustainable and healthy development, limiting will be beautiful
Woods perfume (or spice) garlic forges into the paces of regional characteristic product.
The research that tissue cultures are bred seldom is carried out using Allium fistulosum stem tips about for Yulin perfume (or spice) garlic at present, although
Other related kinds carry out the report of tissue cultures at present, but since Yulin perfume (or spice) garlic kind and other kinds are there are certain difference,
Tissue culture effect is poor.Lack a kind of cultural method and special culture media of the tissue cultural seedlings of free specifically for Yulin perfume (or spice) garlic.
Being disclosed in the information of the background technology part, it is only intended to increase understanding of the overall background of the invention, without answering
It has been the prior art well known to persons skilled in the art when being considered as recognizing or imply that the information is constituted in any form.
【Invention content】
The goal of the invention of the present invention is：For the problems in background technology, a kind of purification and rejuvenation of Yulin perfume (or spice) garlic are provided
Method, is suitble to Yulin perfume (or spice) garlic to carry out tissue culture propagating using the aerosol index point of aerosol index, and the method for the present invention is given birth to gas
Bulb point is sprouted, and morning, growing way be fast, survival rate highest；The Yulin perfume (or spice) garlic bolt stem thickness that tissue culture comes out is strong, and vitality is indomitable, adaptability
Strong feature.
To achieve the goals above, the technical solution adopted by the present invention is as follows：
A kind of purification and rejuvenation method of Yulin perfume (or spice) garlic, includes the following steps：
A. garlic clove of the selection with Yulin perfume (or spice) garlic typical case's hereditary capacity is sowed, when garlic stems stretching leaf sheath, involucre Peng are big
Afterwards, involucre is torn to and is extractd little Hua, Yulin perfume (or spice) garlic Later growth does not harvest garlic stems, postpones Yulin 15-20 days garlic harvest time of perfume (or spice),
When the garlic stems turns yellow, aerosol index is reached maturity, harvesting diameter is more than the aerosol index of 0.5cm, finally gives birth to the gas
Bulb is housed under 5-10 DEG C of environment, spare；
B. aerosol index described in step a is taken, is rinsed with water totally after first removing crust, then surface detoxicating 1- is carried out with alcohol
It 3 times, is subsequently placed into after impregnating 12-18min in the mercuric chloride solution that mass concentration is 0.07-0.15% and takes out, then use sterile water
It rinses 3-6 times, aerosol index is finally peelled off into meat scale under superclean bench anatomical lens, taking-up diameter is 0.1-
The aerosol index point of 1~3 phyllopodium of 1.5mm and band, it is spare；
C. it takes aerosol index described in step b sharp, is inoculated into inducing culture, be first placed in shading training workshop and carry out secretly
Culture 9-14 days, it is 25 ± 5 DEG C to then move into temperature, and intensity of illumination 2000-3000lx, daily light application time 9-12h's is complete
In natural lighting tissue culture workshop, continues culture and grow green young shoot to aerosol index point, obtain unrooted tissue culture bud, it is spare；
D. it takes unrooted tissue culture bud described in step c to move into proliferated culture medium, cultivates 12-18 days, tissue culture bud is grown carefully
To get garlic tissue cultural seedlings of free after root.
Optimization, the sowing was than traditional garlic sowing 10-15 days in advance.
More optimized, the inducing culture is in terms of being basic culture medium by B5, to add the work of following quality-volumetric concentration
Property ingredient：Brassin lactones 0.03-0.2mg/L, jasmonic 0.04-0.1mg/L, phloridzin 0.3-0.9mg/L, activated carbon 1.5-
4g/L, tomato juice 8-15g/L, multi-vitamins 0.1-0.5mg/L.
It advanced optimizes, the proliferated culture medium is in terms of being basic culture medium by B5 or MS, to add following quality-volume
The active constituent of concentration：6-BA 1.2-1.5mg/L,NAA 0.3-0.5mg/L.
Further optimize, the aerosol index point diameter is 0.5-1.0mm.
Further optimize again, 1-3 phyllopodium of the aerosol index point band.
Further optimize again, the air humidity 60-90% of step c shading training workshops.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1. the present invention is before carrying out tissue culture, advanced Yulin perfume (or spice) garlic of being about to carries out cultivation aerosol index, and gas is then given birth to squama again
Stem carries out detoxification pretreatment, can effectively improve garlic kind, prevent garlic from degenerating, and tissue training is carried out to be more suitable for sampling
It supports.The garlic clove with Yulin perfume (or spice) garlic typical case's hereditary capacity, sowing time is selected more generally to produce field plantation garlic and shift to an earlier date 10-15 days,
Appropriate thin planting, planting density, which is more commonly planted, reduces 20-30%, uses sufficient base fertilizer and carries out field management.When garlic stems stretch out leaf sheath,
After involucre Peng is big, involucre is torn to and extractd little Hua, nutrition is made more to focus in aerosol index.The Garlic Growth later stage not
Garlic stems is harvested, 15~20 days garlic harvest times are postponed, waits for that garlic stems turns yellow, is harvested when aerosol index is reached maturity.By planting in advance
It plants, delay harvest, can effectively extend the nutrient accumulation in aerosol index, 10% or more success rate in organizational process can be improved.
Proved through experimental study, by the present invention treated Yulin perfume (or spice) garlic tissue-cultured seedling, optional test field routinely
Cultivate administration technology carries out comparison experimental planting test.As a result garlic bolt per mu yield reaches 3370 public affairs after the plantation of the present invention treated garlic kind
Jin, 2800 kilograms of garlic kind garlic bolt per mu yield not processed by the invention, mu increase production 570 kilograms, and per mu yield improves 20.36%；This
Invention treated garlic kind garlic per mu yield reaches 840 kilograms, 700 kilograms of garlic kind garlic per mu yield not processed by the invention,
140 kilograms of mu volume increase, per mu yield improves 20%.
2. selecting aerosol index point diameter for 0.5-1.0mm when carrying out Garlic Tissue culture in the present invention, gas life
Bulb point is best with 1-3 phyllopodium and with 1-2.By the size of comparative study explant stem apex, with phyllopodium
How much, to bud induce be proliferated impact effect.Test result shows that cutting different size of stem apex under equal conditions trains
It supports, the results showed that, it is cultivated without the stem apex of phyllopodium with 0.1mm, most of stem apex brown stain is at withered death；With 1.0mm
Stem apex with 2-3 phyllopodium is cultivated, and stem apex is sprouted, and morning, growing way be fast, survival rate highest；With 0.5-1.0mm sizes, band
The stem apex of 1-2 phyllopodium is cultivated, more suitable.
3. garlic train inducing culture and proliferated culture medium in seedling cultivating process to the division induction of garlic in vitro tissue, after
The phase influence of proliferation is very big.Select early period of the invention MS culture mediums or B5 medium for basic culture medium, can have maintain from
The not required most of nutritional ingredient of the basic life of plant cell as the culture medium during garlic tissue culture is inadequate with this
's.The present invention is suitble to Garlic Growth to have the various active constituents of facilitation by addition, supplements garlic group in basal nutrient
Nutritional ingredient needed for training, it is balanced nutritious；Using each Substance Interactions, for garlic tissue culture procedures provide it is suitable special
Use culture medium.Found in comparative study, it is best by minimal medium of B5, add various concentration 6-BA, NAA and other activity at
Point, it can effectively be directed to Yulin perfume (or spice) garlic plant characteristic and prepare, garlic budding stem apex number and bud induction rate, tissue-cultured seedling can be effectively improved
Well developed root system.In later stage breeding, ingredient changes required for Yulin perfume (or spice) garlic, if continuing to continue with inducing culture
Proliferation is unfavorable for the further growth of tissue-cultured seedling, therefore the present invention has allocated Yulin for moment during Yulin perfume (or spice) garlic tissue culture
The fragrant dedicated proliferated culture medium of garlic, in terms of being basic culture medium by B5 or MS, add the activity of following quality-volumetric concentration at
Point：6-BA 1.2-1.5mg/L,NAA 0.3-0.5mg/L.The Yulin perfume (or spice) garlic bolt come out through tissue culture of the present invention is strong with stem thickness, raw
Order the features such as power is indomitable, adaptable.
4. being added to brassin lactones in inducing culture of the present invention, has dosage few, can inhibit auxin oxidase
Activity improves the content of plant endogenous auxin, to remarkably promote the elongation growth of garlic.In brassin lactones and culture medium
Auxin such as NAA and other active components such as jasmonic etc., activity has facilitation, collectively promotes garlic group between each other
Knit the growth of middle tissue or tissue-cultured seedling.In the present invention also be added with jasmonic can effectively facilitate Garlic Tissue cell division,
Differentiation, cooperatively promotes Garlic Tissue cell division with 6-BA.Addition jasmonic after can remarkably promote garlic root become mostly and
Growth can excite the expression of defence plant gene, induce the chemical defence of plant, make garlic tissue-cultured seedling from now in planting process
Adaptability enhances, and transplant survival is high.
5. adding certain density activated carbon in inducing culture of the present invention, Garlic Tissue and culture medium can be effectively reduced
Discoloration effectively adsorbs impurity in harmful substance and the culture medium in culture medium, to the detrimental metabolic generated in tissue culture procedures
Product can also play the role of absorption, the survival rate during reducing garlic tissue culture from face.Phloridzin in the present invention can be with
It under culture medium acidic environment, can effectively inhibit harmful microbe activity in culture medium, extend culture medium and go bad, with work
Property charcoal collective effect, it is effective that culture medium is kept to stablize, under the ambient condition for being suitble to Garlic Growth.Culture medium of the present invention with
Basal medium is compared, and Coloring Time can postpone culture medium 5-8 days.
6. the pure major part of tissue culture method process of the present invention is in full natural lighting tissue culture workshop, overcoming traditional tissue culture must
The problem of fluorescent lamp is as plant growth light source must be used, reduce the production costs such as daylight lamp fixture purchase commodity, the electricity charge, garlic group
Cultivate that seedling robust plant, adaptable, transplanting survival rate is high, seedling quality significantly improves.
【Specific implementation mode】
With reference to embodiment, the invention will be further described.
Embodiment 1
A kind of purification and rejuvenation method of Yulin perfume (or spice) garlic, includes the following steps：
A. garlic clove of the selection with Yulin perfume (or spice) garlic typical case's hereditary capacity is sowed, and sowing shifts to an earlier date 10 than traditional garlic sowing
Involucre is torn and is extractd little Hua by it after garlic stems stretches out leaf sheath, involucre Peng is big, and Yulin perfume (or spice) garlic Later growth does not harvest garlic stems,
Postpone 15 days perfume (or spice) garlic harvest times of Yulin, when the garlic stems turns yellow, aerosol index is reached maturity, harvesting diameter is more than 0.5cm's
The aerosol index is finally housed under 5-10 DEG C of environment by aerosol index, spare.
B. aerosol index described in step a is taken, is rinsed with water totally after first removing crust, then surface detoxicating 1 is carried out with alcohol
It is secondary, it is subsequently placed into after impregnating 12min in the mercuric chloride solution that mass concentration is 0.07% and takes out, then with aseptic water washing 3 times, most
Aerosol index is peelled off into meat scale under superclean bench anatomical lens afterwards, taking-up diameter is 1 phyllopodium of 0.1mm and band
Aerosol index point, it is spare.
C. it takes aerosol index described in step b sharp, is inoculated into inducing culture, be first placed in shading training workshop and carry out secretly
The air humidity of culture 9 days, shading training workshop is 60%.It is 25 ± 1 DEG C, intensity of illumination 2000x to then move into temperature, often
In the full natural lighting tissue culture workshop of its light application time 12h, continues culture and grow green young shoot to aerosol index point, obtain unrooted group
Bud is trained, it is spare.
Wherein, inducing culture is in terms of being basic culture medium by B5, to add the active constituent of following quality-volumetric concentration：
It is brassin lactones 0.03mg/L, jasmonic 0.04mg/L, phloridzin 0.3mg/L, activated carbon 1.5g/L, tomato juice 8g/L, compound
Vitamin 0.1mg/L.
D. it takes unrooted tissue culture bud described in step c to move into proliferated culture medium, cultivates 12 days, tissue culture bud grows radicula
Afterwards to get garlic tissue cultural seedlings of free.Wherein, proliferated culture medium is to add following quality-volumetric concentration in terms of B5 basal mediums
Active constituent：6-BA 1.2mg/L,NAA 0.3mg/L.
Embodiment 2
A kind of purification and rejuvenation method of Yulin perfume (or spice) garlic, includes the following steps：
A. garlic clove of the selection with Yulin perfume (or spice) garlic typical case's hereditary capacity is sowed, and sowing shifts to an earlier date 15 than traditional garlic sowing
Involucre is torn and is extractd little Hua by it after garlic stems stretches out leaf sheath, involucre Peng is big, and Yulin perfume (or spice) garlic Later growth does not harvest garlic stems,
Postpone 20 days perfume (or spice) garlic harvest times of Yulin, when the garlic stems turns yellow, aerosol index is reached maturity, harvesting diameter is more than 0.4cm's
The aerosol index is finally housed under 6-10 DEG C of environment by aerosol index, spare.
B. aerosol index described in step a is taken, is rinsed with water totally after first removing crust, then surface detoxicating 3 is carried out with alcohol
It is secondary, it is subsequently placed into after impregnating 18min in the mercuric chloride solution that mass concentration is 0.15% and takes out, then with aseptic water washing 6 times, most
Aerosol index is peelled off into meat scale under superclean bench anatomical lens afterwards, taking-up diameter is 3 phyllopodium of 1.5mm and band
Aerosol index point, it is spare.
C. it takes aerosol index described in step b sharp, is inoculated into inducing culture, be first placed in shading training workshop and carry out secretly
The air humidity of culture 14 days, shading training workshop is 90%.It is 25 ± 5 DEG C, intensity of illumination 3000lx to then move into temperature,
In the full natural lighting tissue culture workshop of daily light application time 9h, continues culture and grow green young shoot to aerosol index point, obtain unrooted
Tissue culture bud, it is spare.
Wherein, inducing culture is in terms of being basic culture medium by B5, to add the active constituent of following quality-volumetric concentration：
Brassin lactones 0.2mg/L, jasmonic 0.1mg/L, phloridzin 0.9mg/L, activated carbon 4g/L, tomato juice 15g/L, the life of compound dimension
Plain 0.5mg/L.
D. it takes unrooted tissue culture bud described in step c to move into proliferated culture medium, cultivates 18 days, tissue culture bud grows radicula
Afterwards to get garlic tissue cultural seedlings of free.Wherein, proliferated culture medium is that in terms of being basic culture medium by MS, it is dense to add following quality-volume
The active constituent of degree：6-BA 1.5mg/L,NAA 0.5mg/L.
Embodiment 3
A kind of purification and rejuvenation method of Yulin perfume (or spice) garlic, includes the following steps：
A. garlic clove of the selection with Yulin perfume (or spice) garlic typical case's hereditary capacity is sowed, and sowing shifts to an earlier date 11 than traditional garlic sowing
Involucre is torn and is extractd little Hua by it after garlic stems stretches out leaf sheath, involucre Peng is big, and Yulin perfume (or spice) garlic Later growth does not harvest garlic stems,
Postpone 16 days perfume (or spice) garlic harvest times of Yulin, when the garlic stems turns yellow, aerosol index is reached maturity, harvesting diameter is more than 0.5cm's
The aerosol index is finally housed under 6-9 DEG C of environment by aerosol index, spare.
B. aerosol index described in step a is taken, is rinsed with water totally after first removing crust, then surface detoxicating 1- is carried out with alcohol
It 3 times, is subsequently placed into after impregnating 15min in the mercuric chloride solution that mass concentration is 0.11% and takes out, then with aseptic water washing 5 times,
Aerosol index is finally peelled off into meat scale under superclean bench anatomical lens, taking-up diameter is 2 phyllogens of 0.7mm and band
The aerosol index point of base, it is spare.
C. it takes aerosol index described in step b sharp, is inoculated into inducing culture, be first placed in shading training workshop and carry out secretly
The air humidity of culture 12 days, shading training workshop is 75%.It is 25 ± 2 DEG C, intensity of illumination 2500lx to then move into temperature,
In the full natural lighting tissue culture workshop of daily light application time 11.5h, continues culture and grow green young shoot to aerosol index point, obtain nothing
Root tissue culture bud, it is spare.
Wherein, inducing culture is in terms of being basic culture medium by B5, to add the active constituent of following quality-volumetric concentration：
Brassin lactones 0.11mg/L, it jasmonic 0.07mg/L, phloridzin 0.6mg/L, activated carbon 2.7g/L, tomato juice 11.5g/L, answers
Close vitamin 0.3mg/L.
D. it takes unrooted tissue culture bud described in step c to move into proliferated culture medium, cultivates 15 days, tissue culture bud grows radicula
Afterwards to get garlic tissue cultural seedlings of free.Wherein, proliferated culture medium is that in terms of being basic culture medium by B5, it is dense to add following quality-volume
The active constituent of degree：6-BA 1.35mg/L,NAA 0.4mg/L.
Embodiment 4
A kind of purification and rejuvenation method of Yulin perfume (or spice) garlic, includes the following steps：
A. garlic clove of the selection with Yulin perfume (or spice) garlic typical case's hereditary capacity is sowed, and sowing shifts to an earlier date 12 than traditional garlic sowing
Involucre is torn and is extractd little Hua by it after garlic stems stretches out leaf sheath, involucre Peng is big, and Yulin perfume (or spice) garlic Later growth does not harvest garlic stems,
Postpone 17 days perfume (or spice) garlic harvest times of Yulin, when the garlic stems turns yellow, aerosol index is reached maturity, harvesting diameter is more than 0.3cm's
The aerosol index is finally housed under 5-10 DEG C of environment by aerosol index, spare.
B. aerosol index described in step a is taken, is rinsed with water totally after first removing crust, then surface detoxicating 3 is carried out with alcohol
It is secondary, it is subsequently placed into after impregnating 16min in the mercuric chloride solution that mass concentration is 0.13% and takes out, then with aseptic water washing 5 times, most
Aerosol index is peelled off into meat scale under superclean bench anatomical lens afterwards, taking-up diameter is 2 phyllopodium of 1.0mm and band
Aerosol index point, it is spare.
C. it takes aerosol index described in step b sharp, is inoculated into inducing culture, be first placed in shading training workshop and carry out secretly
The air humidity of culture 13 days, shading training workshop is 85%.It is 25 ± 4 DEG C, intensity of illumination 2800lx to then move into temperature,
In the full natural lighting tissue culture workshop of daily light application time 11h, continues culture and grow green young shoot to aerosol index point, obtain unrooted
Tissue culture bud, it is spare.
Wherein, inducing culture is in terms of being basic culture medium by B5, to add the active constituent of following quality-volumetric concentration：
It is brassin lactones 0.17mg/L, jasmonic 0.08mg/L, phloridzin 0.8mg/L, activated carbon 3.5g/L, tomato juice 13g/L, compound
Vitamin 0.4mg/L.
D. it takes unrooted tissue culture bud described in step c to move into proliferated culture medium, cultivates 16 days, tissue culture bud grows radicula
Afterwards to get garlic tissue cultural seedlings of free.Wherein, proliferated culture medium is that in terms of being basic culture medium by B5, it is dense to add following quality-volume
The active constituent of degree：6-BA 1.4mg/L,NAA 0.45mg/L.
Embodiment 5
A kind of purification and rejuvenation method of Yulin perfume (or spice) garlic, includes the following steps：
A. garlic clove of the selection with Yulin perfume (or spice) garlic typical case's hereditary capacity is sowed, and sowing shifts to an earlier date 13 than traditional garlic sowing
Involucre is torn and is extractd little Hua by it after garlic stems stretches out leaf sheath, involucre Peng is big, and Yulin perfume (or spice) garlic Later growth does not harvest garlic stems,
Postpone 18 days perfume (or spice) garlic harvest times of Yulin, when the garlic stems turns yellow, aerosol index is reached maturity, harvesting diameter is more than 0.5cm's
The aerosol index is finally housed under 5-8 DEG C of environment by aerosol index, spare.
B. aerosol index described in step a is taken, is rinsed with water totally after first removing crust, then surface detoxicating 2 is carried out with alcohol
It is secondary, it is subsequently placed into after impregnating 14min in the mercuric chloride solution that mass concentration is 0.09% and takes out, then with aseptic water washing 4 times, most
Aerosol index is peelled off into meat scale under superclean bench anatomical lens afterwards, taking-up diameter is 1 phyllopodium of 0.5mm and band
Aerosol index point, it is spare.
C. it takes aerosol index described in step b sharp, is inoculated into inducing culture, be first placed in shading training workshop and carry out secretly
The air humidity of culture 11 days, shading training workshop is 70%.It is 25 ± 3 DEG C, intensity of illumination 2300lx to then move into temperature,
In the full natural lighting tissue culture workshop of daily light application time 10h, continues culture and grow green young shoot to aerosol index point, obtain unrooted
Tissue culture bud, it is spare.
Wherein, inducing culture is in terms of being basic culture medium by B5, to add the active constituent of following quality-volumetric concentration：
It is brassin lactones 0.06mg/L, jasmonic 0.05mg/L, phloridzin 0.5mg/L, activated carbon 2.5g/L, tomato juice 10g/L, compound
Vitamin 0.2mg/L.
D. it takes unrooted tissue culture bud described in step c to move into proliferated culture medium, cultivates 14 days, tissue culture bud grows radicula
Afterwards to get garlic tissue cultural seedlings of free.Wherein, proliferated culture medium is that in terms of being basic culture medium by B5, it is dense to add following quality-volume
The active constituent of degree：6-BA 1.3mg/L,NAA 0.35mg/L.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to
In the covered the scope of the claims of the present invention.
Claims (5)
1. a kind of purification and rejuvenation method of Yulin perfume (or spice) garlic, which is characterized in that include the following steps：
A. garlic clove of the selection with Yulin perfume (or spice) garlic typical case's hereditary capacity is sowed, will after garlic stems stretches out leaf sheath, involucre Peng is big
Involucre tears and extracts little Hua, and Yulin perfume (or spice) garlic Later growth does not harvest garlic stems, postpones Yulin 15-20 days garlic harvest time of perfume (or spice), waits for institute
State that garlic stems turns yellow, aerosol index is when reaching maturity, harvesting diameter is more than the aerosol index of 0.5cm, finally by the aerosol index
It is housed under 5-10 DEG C of environment, it is spare；
B. aerosol index described in step a is taken, is rinsed with water totally after first removing crust, then carries out surface with alcohol and disinfects 1-3 times,
It is subsequently placed into after impregnating 12-18min in the mercuric chloride solution that mass concentration is 0.07-0.15% and takes out, then use aseptic water washing
3-6 times, aerosol index is finally peelled off into meat scale under superclean bench anatomical lens, taking-up diameter is 0.1-1.5mm
And the aerosol index point of 1~3 phyllopodium of band, it is spare；
C. it takes aerosol index described in step b sharp, is inoculated into inducing culture, be first placed in shading training workshop and carry out light culture
9-14 days, it was 25 ± 5 DEG C, intensity of illumination 2000-3000lx to then move into temperature, the full nature of daily light application time 9-12h
In daylighting tissue culture workshop, continues culture and grow green young shoot to aerosol index point, obtain unrooted tissue culture bud, it is spare；The induction training
It is in terms of being basic culture medium by B5, to add the active constituent of following quality-volumetric concentration to support base：Brassin lactones 0.03-
0.2mg/L, it jasmonic 0.04-0.1mg/L, phloridzin 0.3-0.9mg/L, activated carbon 1.5-4g/L, tomato juice 8-15g/L, answers
Close vitamin 0.1-0.5mg/L；
D. it takes unrooted tissue culture bud described in step c to move into proliferated culture medium, cultivates 12-18 days, after tissue culture bud grows radicula,
Up to garlic tissue cultural seedlings of free；The proliferated culture medium is that in terms of being basic culture medium by B5 or MS, it is dense to add following quality-volume
The active constituent of degree：6-BA 1.2-1.5mg/L,NAA 0.3-0.5mg/L.
2. a kind of purification and rejuvenation method of Yulin perfume (or spice) garlic according to claim 1, which is characterized in that the sowing is than conventional
Garlic sowing 10-15 days in advance.
3. a kind of purification and rejuvenation method of Yulin perfume (or spice) garlic according to claim 1, which is characterized in that the aerosol index point
Diameter is 0.5-1.0mm.
4. a kind of purification and rejuvenation method of Yulin perfume (or spice) garlic according to claim 1 or 3, which is characterized in that the gas gives birth to squama
Stem apex 1-3 phyllopodium of band.
5. a kind of purification and rejuvenation method of Yulin perfume (or spice) garlic according to claim 1, which is characterized in that step c shading cultures
The air humidity 60-90% in workshop.
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| CN103918554A (en) * | 2014-04-11 | 2014-07-16 | 山东省农业科学院蔬菜花卉研究所 | Method for inducing tetraploid garlic by using oryzalin |
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| CN103858754B (en) * | 2014-03-05 | 2016-05-18 | 曲靖市滇冠种业有限公司 | A kind of mating system of anti-season garlic new varieties |
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