CN106386206A - Room-temperature inoculation identification method for tobacco resistance to root knot nematode disease - Google Patents
Room-temperature inoculation identification method for tobacco resistance to root knot nematode disease Download PDFInfo
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- CN106386206A CN106386206A CN201610767110.XA CN201610767110A CN106386206A CN 106386206 A CN106386206 A CN 106386206A CN 201610767110 A CN201610767110 A CN 201610767110A CN 106386206 A CN106386206 A CN 106386206A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a room-temperature inoculation identification method for tobacco resistance to a root knot nematode disease. The method comprises the steps that (1) a to-be-identified tobacco variety, a known variety resistant to the root knot nematode disease and a known variety affected with the root knot nematode disease are planted in a tobacco field; (2) tobacco plants are transplanted indoors when the plants send up true leaves, the plants are then inoculated with root knot nematode eggs and grow under the room temperature of 25-30 DEG C; and (3) the disease resistance of each variety to the root knot nematode disease is detected. The invention discloses the room-temperature inoculation identification method for the tobacco resistance to the root knot nematode disease. The method can effectively identify the resistance of the tobacco variety to the root knot nematode disease.
Description
Technical field
The present invention relates to the disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant.
Background technology
Tobacco root-knot is Nicotiana tabacum L. root disease.All can occur in seedling stage and field period, be that root is formed greatly first
The little root nodule not waited, on fibrous root, nascent root nodule is white.When serious whole root system swelling thicker in chicken feet shape, old complaint later stage hollow
Rot, only retain root bark and xylem, wherein comprise the pathogenic nematode of a large amount of different development stages.Tobacco root-knot is distributed
In cigarette districts such as Anhui, Henan, Shandong, Area, Fengyang County, Anhui, some old cigarette ground of Dingyuan are fallen ill universal.Prevention and controls mainly have two kinds：
The first, use d-d mixture fumigating soil；Second, from disease-resistant variety.
Content of the invention
The present invention propose one grow tobacco root-knot nematode resistant disease resistance power room temperature inoculation identification method, can be effective
The disease resistance identifying the root-knot nematode resistant of tobacco bred is strong and weak.
The technical scheme is that and be achieved in that：
The disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant, comprises the following steps：
Step one, plants tobacco bred to be identified, known root-knot nematode resistant kind and known sense root knot line in vega
Parasitosis kind；
Step 2, transplants when tobacco seedlings grow true leaf to interior, inoculates root-knot nematode egg, is 25 DEG C～30 DEG C bars in room temperature
Grow under part；
Step 3, detects that the disease resistance of each kind root-knot nematode resistant is strong and weak.
Further, also planted on vega in step one locality disease-resistant variety, locality in anti-kind and locality
Susceptible variety.
Further, feeling root knot nematode disease kind described in step one for " long neck is yellow ", root-knot nematode resistant kind is
" T1706 " and " NC95 ".
Further, each kind at least plants 5 plants, repeats at least provided with 3 times.
Further, in step 3, the field growing phase checks the aerial partss of cigarette strain, and harvest time checks the aerial partss of cigarette strain
And root, by detect the sickness rate of each kind and/or disease index and/or attacked trees rate and/or have aphid (worm) strain rate and/or
Aphid volume index and/or average individual plant aphid amount and/or hundred plants of worm amounts and/or have ovum strain rate to detect the anti-of each kind root-knot nematode resistant
Characteristic of disease is strong and weak.
Further, sickness rate=(morbidity strain number/investigation total strain number) * 100%；Disease index=[Σ (diseased plants at different levels or leaf
Number this sick level value of *)/(investigation total strain number or the highest value of number of sheets *)] * 100；Attacked trees rate=(the total strain of killed strain number/investigation
Number) * 100%；There is aphid (worm) strain rate=[having aphid (worm) strain number/investigation total strain number] * 100%；Aphid volume index=[Σ (leaves at different levels
Number this class value of *)/(investigation total strain number or the highest value of number of sheets *)] * 100；Average individual plant aphid amount=total aphid amount/total strain number；Hundred
Strain worm amount=(total worm amount/total strain number) * 100；There is ovum strain rate=(having ovum strain number/investigation total strain number) * 100%,.
Further, the root knot nematode disease Severity gradation of the aerial partss of cigarette strain：0 grade：Plant strain growth is normal；1 grade：Plant
Growth is normal, Ye Lv, tip segment jaundice, but does not do point；3 grades：Diseased plant than healthy tree short a quarter to 1/3rd, or
Blade slightly does point, dry side；5 grades：Diseased plant is shorter by 1/1st to two/3rd than healthy tree, or most of blade do point, dry side or
There is withered and yellow speckle；7 grades：Diseased plant is shorter by more than 1/2nd than healthy tree, and whole blades are done point, dry side or had withered and yellow speckle；9 grades：Plant is tight
Downgrade, Herb blade is substantially withered again.
Further, the root knot nematode disease Severity gradation of the root of cigarette strain：0 grade：Root is normal；1 grade：Below a quarter
A small amount of root knot is had on root；3 grades：A quarter has a small amount of root knot on 1/3rd；5 grades：1/1st to two/3rd
On have root knot；7 grades：There is root knot on more than 1/2nd roots, a small amount of secondary root produces root knot；9 grades：All upper (inclusions time
Take root) cover with root knot.
Further, root-knot nematode egg be using susceptible tomato variety " Rutgers " inoculation reproduction root-knot nematode 25
At～30 DEG C, growth obtains for 55～60 days.
Beneficial effects of the present invention are：The present invention proposes a disease resistance power room temperature growing tobacco root-knot nematode resistant and inoculates
Authentication method, the disease resistance that can effectively identify the root-knot nematode resistant of tobacco bred is strong and weak.
Specific embodiment
For more fully understanding the present invention, below by following examples, the present invention is made specifically to illustrate further, but not
Can be regarded as limitation of the invention, some made according to foregoing invention content for those skilled in the art are nonessential
Improve and adjustment, be also considered as being within the scope of the present invention.
Embodiment 1
The disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant, comprises the following steps：
Step one, plants tobacco bred to be identified, known root-knot nematode resistant kind and known sense root knot line in vega
Parasitosis kind；Each kind plants 5 plants, 3 repetitions of setting, and described sense root knot nematode disease kind is " long neck is yellow ", anti-root knot line
Parasitosis kind is " T1706 " and " NC95 "
Step 2, transplants when tobacco seedlings grow true leaf to interior, inoculates root-knot nematode egg, raw under the conditions of room temperature is 25 DEG C
Long；Wherein, root-knot nematode egg is to be grown at 25 DEG C using the root-knot nematode of susceptible tomato variety " Rutgers " inoculation reproduction
Obtain within 60 days；
Step 3, the field growing phase checks the aerial partss of cigarette strain, and harvest time checks aerial partss and the root of cigarette strain, leads to
Cross the sickness rate detecting each kind and/or disease index and/or attacked trees rate and/or have aphid (worm) strain rate and/or aphid volume index
And/or average individual plant aphid amount and/or hundred plants of worm amounts and/or have ovum strain rate to detect that the disease resistance of each kind root-knot nematode resistant is strong
Weak, sickness rate=(morbidity strain number/investigation total strain number) * 100%；Disease index=[Σ (diseased plants at different levels or this sick level of number of sheets *
Value)/(investigation total strain number or the highest value of number of sheets *)] * 100；Attacked trees rate=(killed strain number/investigation total strain number) * 100%；Have
Aphid (worm) strain rate=[having aphid (worm) strain number/investigation total strain number] * 100%；Aphid volume index=[Σ (this class value of numbers of sheets * at different levels)/
(investigation total strain number or the highest value of number of sheets *)] * 100；Average individual plant aphid amount=total aphid amount/total strain number；Hundred plants of worm amount=(total worms
Amount/total strain number) * 100；There are ovum strain rate=(having ovum strain number/investigation total strain number) * 100%, the root-knot nematode of the aerial partss of cigarette strain
Sick Severity gradation：0 grade：Plant strain growth is normal；1 grade：Plant strain growth is normal, Ye Lv, tip segment jaundice, but does not do
Point；3 grades：Diseased plant than healthy tree short a quarter to 1/3rd, or blade slightly does point, dry side；5 grades：Diseased plant is shorter by three than healthy tree
1/1 mono- to two, or most of blade does point, dry side or has withered and yellow speckle；7 grades：Diseased plant than healthy tree short 1/2nd with
On, whole blades are done point, dry side or are had withered and yellow speckle；9 grades：Plant is seriously downgraded, and Herb blade is substantially withered, the root of cigarette strain
Root knot nematode disease Severity gradation：0 grade：Root is normal；1 grade：A small amount of root knot is had on root below a quarter；3 grades：A quarter
There is a small amount of root knot on 1/3rd；5 grades：There is root knot on 1/1st to two/3rd；7 grades：More than/2nd roots
On have root knot, in a small amount of secondary root produce root knot；9 grades：All upper (inclusion secondary roots) cover with root knot.
Embodiment 2
Also planted on vega in step one locality disease-resistant variety, locality in anti-kind and locality susceptible product
Kind.
The disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant, comprises the following steps：
Step one, plants tobacco bred to be identified, known root-knot nematode resistant kind, known sense root knot nematode disease in vega
Kind, local disease-resistant variety, in locality anti-kind and locality susceptible variety, each kind plants 6 plants, arranges 4 times
Repeat；
Step 2, transplants when tobacco seedlings grow true leaf to interior, inoculates root-knot nematode egg, raw under the conditions of room temperature is 30 DEG C
Long；Wherein, root-knot nematode egg is derived from indoor plantation sick soil；
Step 3, the field growing phase checks the aerial partss of cigarette strain, and harvest time checks aerial partss and the root of cigarette strain, leads to
Cross the sickness rate detecting each kind and/or disease index and/or attacked trees rate and/or have aphid (worm) strain rate and/or aphid volume index
And/or average individual plant aphid amount and/or hundred plants of worm amounts and/or have ovum strain rate to detect that the disease resistance of each kind root-knot nematode resistant is strong
Weak, sickness rate=(morbidity strain number/investigation total strain number) * 100%；Disease index=[Σ (diseased plants at different levels or this sick level of number of sheets *
Value)/(investigation total strain number or the highest value of number of sheets *)] * 100；Attacked trees rate=(killed strain number/investigation total strain number) * 100%；Have
Aphid (worm) strain rate=[having aphid (worm) strain number/investigation total strain number] * 100%；Aphid volume index=[Σ (this class value of numbers of sheets * at different levels)/
(investigation total strain number or the highest value of number of sheets *)] * 100；Average individual plant aphid amount=total aphid amount/total strain number；Hundred plants of worm amount=(total worms
Amount/total strain number) * 100；There are ovum strain rate=(having ovum strain number/investigation total strain number) * 100%, the root-knot nematode of the aerial partss of cigarette strain
Sick Severity gradation：0 grade：Plant strain growth is normal；1 grade：Plant strain growth is normal, Ye Lv, tip segment jaundice, but does not do
Point；3 grades：Diseased plant than healthy tree short a quarter to 1/3rd, or blade slightly does point, dry side；5 grades：Diseased plant is shorter by three than healthy tree
1/1 mono- to two, or most of blade does point, dry side or has withered and yellow speckle；7 grades：Diseased plant than healthy tree short 1/2nd with
On, whole blades are done point, dry side or are had withered and yellow speckle；9 grades：Plant is seriously downgraded, and Herb blade is substantially withered, the root of cigarette strain
Root knot nematode disease Severity gradation：0 grade：Root is normal；1 grade：A small amount of root knot is had on root below a quarter；3 grades：A quarter
There is a small amount of root knot on 1/3rd；5 grades：There is root knot on 1/1st to two/3rd；7 grades：More than/2nd roots
On have root knot, in a small amount of secondary root produce root knot；9 grades：All upper (inclusion secondary roots) cover with root knot.
Embodiment 3
The disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant, comprises the following steps：
Step one, plants tobacco bred to be identified, known root-knot nematode resistant kind and known sense root knot line in vega
Parasitosis kind；Each kind plants 7 plants, repeats at least provided with 5 times；
Step 2, transplants when tobacco seedlings grow true leaf to interior, inoculates root-knot nematode egg, raw under the conditions of room temperature is 28 DEG C
Long；Wherein, root-knot nematode egg is to be grown at 28 DEG C using the root-knot nematode of susceptible tomato variety " Rutgers " inoculation reproduction
Obtain within 58 days；
Step 3, the field growing phase checks the aerial partss of cigarette strain, and harvest time checks aerial partss and the root of cigarette strain, leads to
Cross the sickness rate detecting each kind and/or disease index and/or attacked trees rate and/or have aphid (worm) strain rate and/or aphid volume index
And/or average individual plant aphid amount and/or hundred plants of worm amounts and/or have ovum strain rate to detect that the disease resistance of each kind root-knot nematode resistant is strong
Weak, sickness rate=(morbidity strain number/investigation total strain number) * 100%；Disease index=[Σ (diseased plants at different levels or this sick level of number of sheets *
Value)/(investigation total strain number or the highest value of number of sheets *)] * 100；Attacked trees rate=(killed strain number/investigation total strain number) * 100%；Have
Aphid (worm) strain rate=[having aphid (worm) strain number/investigation total strain number] * 100%；Aphid volume index=[Σ (this class value of numbers of sheets * at different levels)/
(investigation total strain number or the highest value of number of sheets *)] * 100；Average individual plant aphid amount=total aphid amount/total strain number；Hundred plants of worm amount=(total worms
Amount/total strain number) * 100；There are ovum strain rate=(having ovum strain number/investigation total strain number) * 100%, the root-knot nematode of the aerial partss of cigarette strain
Sick Severity gradation：0 grade：Plant strain growth is normal；1 grade：Plant strain growth is normal, Ye Lv, tip segment jaundice, but does not do
Point；3 grades：Diseased plant than healthy tree short a quarter to 1/3rd, or blade slightly does point, dry side；5 grades：Diseased plant is shorter by three than healthy tree
1/1 mono- to two, or most of blade does point, dry side or has withered and yellow speckle；7 grades：Diseased plant than healthy tree short 1/2nd with
On, whole blades are done point, dry side or are had withered and yellow speckle；9 grades：Plant is seriously downgraded, and Herb blade is substantially withered, the root of cigarette strain
Root knot nematode disease Severity gradation：0 grade：Root is normal；1 grade：A small amount of root knot is had on root below a quarter；3 grades：A quarter
There is a small amount of root knot on 1/3rd；5 grades：There is root knot on 1/1st to two/3rd；7 grades：More than/2nd roots
On have root knot, in a small amount of secondary root produce root knot；9 grades：All upper (inclusion secondary roots) cover with root knot.
The sickness rate of tobacco bred to be identified and/or disease index and/or attacked trees rate and/or have aphid (worm) strain rate and/
Or aphid volume index and/or average individual plant aphid amount and/or hundred plants of worm amounts and/or have ovum strain rate and known root-knot nematode resistant Nicotiana tabacum L. product
Kind and/or the sickness rate of root-knot nematode resistant tobacco bred of locality and/or disease index and/or attacked trees rate and/or have aphid
(worm) strain rate and/or aphid volume index and/or average individual plant aphid amount and/or hundred plants of worm amounts and/or have ovum strain rate same or like
Kind be root-knot nematode resistant kind；
The sickness rate of tobacco bred to be identified and/or disease index and/or attacked trees rate and/or have aphid (worm) strain rate and/
Or aphid volume index and/or average individual plant aphid amount and/or hundred plants of worm amounts and/or the middle root-knot nematode resistant cigarette having ovum strain rate and locality
The sickness rate of grass product kind and/or disease index and/or attacked trees rate and/or have aphid (worm) strain rate and/or aphid volume index and/or flat
All individual plant aphid amount and/or hundred plants of worm amounts and/or have the same or like kind of ovum strain rate to be middle root-knot nematode resistant kind；
The sickness rate of tobacco bred to be identified and/or disease index and/or attacked trees rate and/or have aphid (worm) strain rate and/
Or aphid volume index and/or average individual plant aphid amount and/or hundred plants of worm amounts and/or have ovum strain rate with known sense root knot nematode disease Nicotiana tabacum L. product
Kind and/or the sickness rate of sense root knot nematode disease tobacco bred of locality and/or disease index and/or attacked trees rate and/or have aphid
(worm) strain rate and/or aphid volume index and/or average individual plant aphid amount and/or hundred plants of worm amounts and/or have ovum strain rate same or like
Kind be sense root knot nematode disease kind.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement made etc., should be included within the scope of the present invention.
Claims (9)
1. the disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant is it is characterised in that comprise the following steps：
Step one, plants tobacco bred to be identified, known root-knot nematode resistant kind and known sense root knot nematode disease in vega
Kind；
Step 2, transplants when tobacco seedlings grow true leaf to interior, inoculates root-knot nematode egg, under the conditions of room temperature is 25 DEG C～30 DEG C
Growth；
Step 3, detects that the disease resistance of each kind root-knot nematode resistant is strong and weak.
2. the disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant as claimed in claim 1, its feature exists
In：Also planted on vega in step one locality disease-resistant variety, locality in anti-kind and locality susceptible variety.
3. the disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant as claimed in claim 1 or 2, its feature
It is：Sense root knot nematode disease kind described in step one is " long neck yellow ", root-knot nematode resistant kind be " T1706 " and
“NC95”.
4. the disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant as claimed in claim 1 or 2, its feature
It is：Each kind at least plants 5 plants, repeats at least provided with 3 times.
5. the disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant as claimed in claim 1 or 2, its feature
It is：In step 3, the field growing phase checks the aerial partss of cigarette strain, and harvest time checks aerial partss and the root of cigarette strain, leads to
Cross the sickness rate detecting each kind and/or disease index and/or attacked trees rate and/or have aphid (worm) strain rate and/or aphid volume index
And/or average individual plant aphid amount and/or hundred plants of worm amounts and/or have ovum strain rate to detect that the disease resistance of each kind root-knot nematode resistant is strong
Weak.
6. the disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant as claimed in claim 5, its feature exists
In：Sickness rate=(morbidity strain number/investigation total strain number) * 100%；Disease index=[Σ (diseased plants at different levels or this sick level of number of sheets *
Value)/(investigation total strain number or the highest value of number of sheets *)] * 100；Attacked trees rate=(killed strain number/investigation total strain number) * 100%；Have
Aphid (worm) strain rate=[having aphid (worm) strain number/investigation total strain number] * 100%；Aphid volume index=[Σ (this class value of numbers of sheets * at different levels)/
(investigation total strain number or the highest value of number of sheets *)] * 100；Average individual plant aphid amount=total aphid amount/total strain number；Hundred plants of worm amount=(total worms
Amount/total strain number) * 100；There is ovum strain rate=(having ovum strain number/investigation total strain number) * 100%.
7. the disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant as claimed in claim 6, its feature exists
In：The root knot nematode disease Severity gradation of the aerial partss of cigarette strain：0 grade：Plant strain growth is normal；1 grade：Plant strain growth is substantially just
Often, Ye Lv, tip segment jaundice, but do not do point；3 grades：Diseased plant than healthy tree short a quarter to 1/3rd, or blade is slightly done
Point, dry side；5 grades：Diseased plant is shorter by 1/1st to two/3rd than healthy tree, or most of blade is done point, dry side or had withered and yellow speckle；7
Level：Diseased plant is shorter by more than 1/2nd than healthy tree, and whole blades are done point, dry side or had withered and yellow speckle；9 grades：Plant is seriously downgraded, Herb
Blade is substantially withered.
8. the disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant as claimed in claim 6, its feature exists
In：The root knot nematode disease Severity gradation of the root of cigarette strain：0 grade：Root is normal；1 grade：A small amount of root is had on root below a quarter
Knot；3 grades：A quarter has a small amount of root knot on 1/3rd；5 grades：There is root knot on 1/1st to two/3rd；7 grades：
There is root knot on more than 1/2nd roots, a small amount of secondary root produces root knot；9 grades：All upper (inclusion secondary roots) cover with root knot.
9. the disease resistance power room temperature inoculation identification method of Nicotiana tabacum L. root-knot nematode resistant as claimed in claim 1 or 2, its feature
It is：Root-knot nematode egg is raw at 25～30 DEG C using the root-knot nematode of susceptible tomato variety " Rutgers " inoculation reproduction
Length obtains for 55～60 days.
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| CN201610767110.XA CN106386206A (en) | 2016-08-30 | 2016-08-30 | Room-temperature inoculation identification method for tobacco resistance to root knot nematode disease |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107771561A (en) * | 2017-10-14 | 2018-03-09 | 云南省烟草农业科学研究院 | A kind of anti-/ tolerance to diseases authentication method of javanese root knot nematode |
| CN108307949A (en) * | 2018-02-26 | 2018-07-24 | 广西大学 | A method of the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety |
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| CN104082124A (en) * | 2014-06-30 | 2014-10-08 | 章清杞 | Selective breeding method of high-resistance tobacco variety |
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| CN104082124A (en) * | 2014-06-30 | 2014-10-08 | 章清杞 | Selective breeding method of high-resistance tobacco variety |
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| 任广伟等: "《烟草病虫害分级及调查方法，GB/T2322》", 《中华人民共和国国家标准》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107771561A (en) * | 2017-10-14 | 2018-03-09 | 云南省烟草农业科学研究院 | A kind of anti-/ tolerance to diseases authentication method of javanese root knot nematode |
| CN108307949A (en) * | 2018-02-26 | 2018-07-24 | 广西大学 | A method of the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety |
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