CN108307949A - A method of the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety - Google Patents
A method of the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety Download PDFInfo
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- CN108307949A CN108307949A CN201810159831.1A CN201810159831A CN108307949A CN 108307949 A CN108307949 A CN 108307949A CN 201810159831 A CN201810159831 A CN 201810159831A CN 108307949 A CN108307949 A CN 108307949A
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- root
- knot nematode
- cucumber
- knot
- screening
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- 238000000034 method Methods 0.000 title claims abstract description 30
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 title claims abstract description 25
- 238000012216 screening Methods 0.000 title claims abstract description 16
- 241000244206 Nematoda Species 0.000 title claims abstract description 15
- 244000299906 Cucumis sativus var. sativus Species 0.000 title 1
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 24
- 241000243785 Meloidogyne javanica Species 0.000 claims abstract description 18
- 238000004043 dyeing Methods 0.000 claims abstract description 14
- 201000010099 disease Diseases 0.000 claims abstract description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 9
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims abstract description 5
- 240000003768 Solanum lycopersicum Species 0.000 claims abstract description 4
- 239000000725 suspension Substances 0.000 claims description 19
- 102000002322 Egg Proteins Human genes 0.000 claims description 7
- 108010000912 Egg Proteins Proteins 0.000 claims description 7
- 239000002775 capsule Substances 0.000 claims description 7
- 210000004681 ovum Anatomy 0.000 claims description 7
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 6
- 230000012447 hatching Effects 0.000 claims description 6
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 6
- 229960005076 sodium hypochlorite Drugs 0.000 claims description 6
- 241001677344 Meloidogyne ethiopica Species 0.000 claims description 5
- 239000000975 dye Substances 0.000 claims description 5
- SGHZXLIDFTYFHQ-UHFFFAOYSA-L Brilliant Blue Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 SGHZXLIDFTYFHQ-UHFFFAOYSA-L 0.000 claims description 3
- 235000012745 brilliant blue FCF Nutrition 0.000 claims description 3
- 239000004161 brilliant blue FCF Substances 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 3
- 208000015181 infectious disease Diseases 0.000 claims description 3
- 235000015170 shellfish Nutrition 0.000 claims description 3
- 238000010828 elution Methods 0.000 claims description 2
- 230000035558 fertility Effects 0.000 claims description 2
- 238000005286 illumination Methods 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 238000011081 inoculation Methods 0.000 abstract description 3
- 238000009331 sowing Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 8
- 238000004382 potting Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 241000219193 Brassicaceae Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 241000227653 Lycopersicon Species 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention belongs to cucumber resistant variety screening technique field more particularly to a kind of methods of the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety.A method of the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety includes the following steps：Sowing cucumber seed collects root-knot nematode, inoculation, dyeing and calculates disease index, and the anti-root-knot nematode grade of different cucumber varieties is evaluated according to disease index.The method of the present invention is simple to operate, and workload greatly reduces, efficient；It is not limited by a space, the kind disposably screened is up to tens to hundreds of, the resistance screening especially suitable for the common crop such as cucumber, tomato.
Description
Technical field
The invention belongs to cucumber resistant variety screening technique field more particularly to a kind of extensive quickly screening and assessment cucumber
The method of the anti-root-knot nematode of kind.
Background technology
Root-knot nematode is one of important pathogen of plant, can cause the world agriculture annual underproduction 24.5%, economic loss
Seriously (Taylor and Sa Sai, 1983).Root-knot nematode host range is extensive, can colonize in including monocotyledon, dicotyledon,
In 114 3000 various plants of section including herbaceous plant and xylophyta, wherein especially with Solanaceae, Curcurbitaceae, Cruciferae etc.
Plant morbidity is relatively universal (Liu Weizhi, 2000).
There is no good control method, resistant variety at present is a kind of cost-effective method applied in production, but
In the selection and breeding of disease-resistant variety or stock, screening and qualification process, method for potted, but the heavy workload of pot experiment are usually utilized,
Occupied space is big, and the influence factor being subject to is more, make troubles with the accuracy of result to research work etc., it is therefore, high-throughput fast
The fast anti-root-knot nematode kind method of screening and identification has important application value.
Being disclosed in the information of the background technology part, it is only intended to increase understanding of the overall background of the invention, without answering
It has been the prior art well known to persons skilled in the art when being considered as recognizing or imply that the information is constituted in any form.
Invention content
The object of the present invention is to provide a kind of method of the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety, with gram
The heavy workload of existing pot experiment is taken, occupied space is big, and the influence factor being subject to is more, makes troubles to work and influences to study
As a result the defect of accuracy.
To achieve the goals above, the present invention adopts the following technical solutions realizes：
A method of the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety includes the following steps：
(1) full cucumber seeds are put into seed and sprout the position that can be sowed close to middle part in bag, every bag is sowed 2,
1 plant is stayed after emergence, tap water 1-1.5mL is then added, and then seed sprouting bag is put into suspension clamp, seed sprouts bag humidity
100%；
(2) after planting the 6th day, the Tomato Root System for the infection root-knot nematode cultivated in advance is taken, is detached using sodium-hypochlorite process
Root-knot nematode egg is collected, and in making its hatching on the graceful funnel of shellfish, collects the fresh root-knot nematode second instar larvae of same day hatching, waits for
When root system lateral root is grown to 3-4cm, you can inoculation；
(3) it takes out seed and sprouts bag, draw root-knot nematode suspension with pipettor, each root system is inoculated with 1mL, seed is sprouted
It sends out bag and is horizontally arranged 10-15h；
(4) 12-15 days after being inoculated with, the visible root knot of naked eyes is dyed using dyeing liquor, egg capsule outside record root knot；
(5) the egg capsule quantity on each root system is recorded, then the ovum in egg capsule is eluted with sodium-hypochlorite process, calculates the number of ovum
Amount, determines root knot ratio according to Zeck grade scales, calculates disease index, and anti-of different cucumber varieties are evaluated according to disease index
Tie lines insect pest situation condition.
Preferably, in step (3) in root-knot nematode suspension root-knot nematode second instar larvae a concentration of 300/mL.
Preferably, dyeing liquor is 75mg/L Erioglaucine Dye dyeing liquors or a concentration of 4% food in step (4)
Grade dyeing liquid.
Compared with prior art, the device have the advantages that being：
Compared with traditional potting, method of the invention is simple to operate, and workload greatly reduces, efficient；Not by sky
Between limit, the kind disposably screened up to tens to hundreds of, especially suitable for the common crop such as cucumber, tomato resistance sieve
Choosing.
Description of the drawings
Fig. 1 is that seed sprouts bag；
Fig. 2 is suspension clamp；
Fig. 3 is suspension clamp frame；
Fig. 4 is that cucumber variety length to be identified is emerged；
Fig. 5 is root system to be seeded；
Fig. 6 is to observe apparent blue egg capsule after dyeing under root magnifying glass.
Specific implementation mode
With reference to specific embodiment, further details of elaboration is made to the present invention, but embodiments of the present invention are not
It is confined to the range of embodiment expression.
Embodiment 1：
A method of the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety includes the following steps：
(1) full cucumber seeds are put into seed and sprout the position that can be sowed in bag (see Fig. 1), every bag is sowed 2, is gone out
1 plant is stayed after seedling, tap water 1-1.5mL is then added, and then seed sprouting bag is put into suspension clamp (see Fig. 2), seed sprouts bag
Humidity 100%, then suspension clamp is placed on suspension clamp frame (see Fig. 3)；
(2) after planting the 6th day, the Tomato Root System for the infection root-knot nematode cultivated in advance is taken, is detached using sodium-hypochlorite process
Root-knot nematode egg is collected, and in making its hatching on the graceful funnel of shellfish, collects the fresh root-knot nematode second instar larvae of same day hatching, waits for
When root system lateral root is grown to 3-4cm, you can inoculation；
(3) it takes out seed and sprouts bag, draw root-knot nematode suspension with pipettor, each root system is inoculated with 1mL, seed is sprouted
It sends out bag and is horizontally arranged 10-15h；A concentration of 300/mL of root-knot nematode second instar larvae in root-knot nematode suspension；
(4) 12-15 days after being inoculated with, the visible root knot of naked eyes is dyed using dyeing liquor, egg sac number outside record root knot
Amount；The dyeing liquor is 75mg/L Erioglaucine Dye dyeing liquors or a concentration of 4% food grade dyes dyeing liquor；
(5) root knot ratio is determined according to Zeck grade scales, calculates disease index, different cucumber are evaluated according to disease index
The anti-root-knot nematode situation of kind；Also using the ovum in sodium-hypochlorite process elution egg capsule, the quantity of ovum is calculated, different cultivars is compared
Influence to root-knot nematode fertility, to evaluate varietal resistance.
For evaluating the resistance of 50 cucumber varieties：
(1) conventional basin support method, 5 repetitions need to prepare 250, small basin, and soil and husky and sterilizing works are (if per basin
200cm3, need to prepare native husky mixing 50000cm3), a series of activities such as dress basin need to take 10-14 hours, and the present invention is then
This part-time can be saved, efficiency is improved.
(2) from spatially comparing, conventional potting needs to take up an area (per small basin 50cm2, 12500cm is needed altogether2), and it is of the invention
Then only need 4690cm2(36.3 × 32.3=1172.49 of each suspension clamp frame area, each frame can put 30-35 suspension clamp, each
2 young plant in suspension clamp, therefore, 250 young plants need 4 suspension clamp framves, account for area 4690cm altogether2), and conventional potting area occupied is this
2.6 times of method, method of the invention save big quantity space.
(3) work is dyed, conventional method, which needs to collect from basin, root system and cleans, and per basin 0.08 hour, needs altogether 20 small
When, while root system is easily damaged in operation, and method using the present invention not only can keep root system complete, simultaneously also
Save the step for root system is collected.
The description of the aforementioned specific exemplary embodiment to the present invention is in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to the above instruction, can much be changed
And variation.The purpose of selecting and describing the exemplary embodiment is that explaining the specific principle of the present invention and its actually answering
With so that those skilled in the art can realize and utilize the present invention a variety of different exemplary implementation schemes and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (3)
1. a kind of method of the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety, which is characterized in that include the following steps：
(1) full cucumber seeds are put into seed and sprout the position that can be sowed in bag, every bag is sowed 2, is then added originally
Then seed sprouting bag is put into suspension clamp by water 1-1.5mL, seed sprouts bag humidity 100%；
(2) at after planting the 6th day, the Tomato Root System for the infection root-knot nematode cultivated in advance is taken, is detached and is received using sodium-hypochlorite process
Collect root-knot nematode egg, and ovum is placed on the graceful funnel of shellfish makes its hatching, when root system lateral root is grown to 3-4cm, collects same day hatching
Fresh root-knot nematode second instar larvae, you can root system to be identified is inoculated with；
(3) it when being inoculated with, needs first to take out seed sprouting bag from suspension clamp, seed sprouting bag is horizontally arranged, is drawn with pipettor
Root-knot nematode suspension, each root system are inoculated with 1mL, on the root system being uniformly inoculated with, then place 10-15h, are then transferred in suspension clamp
Culture ensures the illumination condition of light dark=12/12；
(4) 12-15 days after being inoculated with, the visible root knot of naked eyes is dyed using dyeing liquor, egg capsule quantity outside record root knot；
(5) root knot ratio is determined according to Zeck grade scales, calculates disease index, different cucumber varieties are evaluated according to disease index
Anti- root-knot nematode situation；Or using the ovum in sodium-hypochlorite process elution egg capsule, the quantity of ovum is calculated, compares different cultivars to root knot
The influence of nematode fertility, to evaluate varietal resistance.
2. the method for the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety according to claim 1, feature exist
In, in step (3) in root-knot nematode suspension root-knot nematode second instar larvae a concentration of 300/mL.
3. the method for the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety according to claim 1, feature exist
In dyeing liquor is 75mg/L Erioglaucine Dye dyeing liquors or a concentration of 4% food grade dyes dyeing liquor in step (4).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810159831.1A CN108307949A (en) | 2018-02-26 | 2018-02-26 | A method of the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety |
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| CN201810159831.1A CN108307949A (en) | 2018-02-26 | 2018-02-26 | A method of the extensive quickly anti-root-knot nematode of screening and assessment cucumber variety |
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| CN108307949A true CN108307949A (en) | 2018-07-24 |
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|---|---|---|---|---|
| CN105165434A (en) * | 2015-10-14 | 2015-12-23 | 广西壮族自治区农业科学院植物保护研究所 | Method for identifying solanum lycopersicum seedling stage meloidogyne disease resistibility |
| CN106386206A (en) * | 2016-08-30 | 2017-02-15 | 中国烟草总公司广东省公司 | Room-temperature inoculation identification method for tobacco resistance to root knot nematode disease |
| CN106718181A (en) * | 2017-02-10 | 2017-05-31 | 北京农学院 | A kind of method of plant identification to root knot nematode resistance |
| CN106718180A (en) * | 2016-11-29 | 2017-05-31 | 黑龙江省农业科学院克山分院 | A kind of resistance method of high frequency zone potato to root-knot nematode |
| CN206808005U (en) * | 2017-06-26 | 2017-12-29 | 宋洋 | A kind of new seed sprouts and root system observation bag |
| CN207022425U (en) * | 2017-07-31 | 2018-02-23 | 山东省农作物种质资源中心 | Seed sprouts bag |
-
2018
- 2018-02-26 CN CN201810159831.1A patent/CN108307949A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105165434A (en) * | 2015-10-14 | 2015-12-23 | 广西壮族自治区农业科学院植物保护研究所 | Method for identifying solanum lycopersicum seedling stage meloidogyne disease resistibility |
| CN106386206A (en) * | 2016-08-30 | 2017-02-15 | 中国烟草总公司广东省公司 | Room-temperature inoculation identification method for tobacco resistance to root knot nematode disease |
| CN106718180A (en) * | 2016-11-29 | 2017-05-31 | 黑龙江省农业科学院克山分院 | A kind of resistance method of high frequency zone potato to root-knot nematode |
| CN106718181A (en) * | 2017-02-10 | 2017-05-31 | 北京农学院 | A kind of method of plant identification to root knot nematode resistance |
| CN206808005U (en) * | 2017-06-26 | 2017-12-29 | 宋洋 | A kind of new seed sprouts and root system observation bag |
| CN207022425U (en) * | 2017-07-31 | 2018-02-23 | 山东省农作物种质资源中心 | Seed sprouts bag |
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| Title |
|---|
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