CN106370654A - Solid state linear continuous gradient measurement method - Google Patents
Solid state linear continuous gradient measurement method Download PDFInfo
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- CN106370654A CN106370654A CN201610726776.0A CN201610726776A CN106370654A CN 106370654 A CN106370654 A CN 106370654A CN 201610726776 A CN201610726776 A CN 201610726776A CN 106370654 A CN106370654 A CN 106370654A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/80—Indicating pH value
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Abstract
The application discloses a solid state linear continuous gradient measurement method including the steps of: a) coating a solid carrier with a known substance having linear continuous gradient, wherein the known substance serves as an evidence for quantitative comparison; b) with the gradient values of the known substance as independent variables and parameter values of the gradient positions as dependent variables, drawing a standard calibration line; c) arranging an unknown to-be-measured substance in the system of which the conditions are same as those of the known substance so as to obtain the parameter values of the unknown to-be-measured substance; and d) calculating the gradient value of the unknown to-be-measured substance by means of the parameter value of the unknown to-be-measured substance and the standard calibration line. The solid state linear continuous gradient measurement method enables measurement to be quicker, more accurate and more convenient.
Description
Technical field
The invention belongs to analysis and testing technology field, the linear continuous gradient measuring method of more particularly to a kind of solid-state.
Background technology
Precise treatment, high-quality and high-performance are increasingly tended in the development of modern science and technology and industry, and wherein, e measurement technology is
Become the excelsior key of the mankind, including physical measurement and chemical measurement etc..
In prior art, typically adopt the spectral measurement methodses of the direct or indirect chromogenic reaction of liquid, specifically, utilize
The detection comprising visible ray, infrared ray and ultraviolet between 200 nanometers to 950 nanometers for the spectral wavelength ranges, however this kind of
Technology will reach more rapid, more accurate, more convenience requirement, has certain limitation.
Content of the invention
For solving the above problems, the invention provides a kind of solid-state linear continuous gradient measuring method, can make to measure
Journey is more rapid, more precisely and more convenience.
A kind of solid-state linear continuous gradient measuring method that the present invention provides includes:
On solid-state carrier, coating has the known substance of LINEAR CONTINUOUS gradient, using described known substance as quantitative contrast
Foundation；
Using each Grad of described known substance as independent variable, using the parameter value of each described gradient locations as because
Variable, makes standard calibration curve；
By unknown material to be checked put into described known substance identical condition system in and obtain described unknown thing to be checked
The parameter value of matter；
Using parameter value and the described standard calibration curve of described unknown material to be checked, calculate the ladder of described unknown material to be checked
Angle value.
Preferably, in above-mentioned solid-state linear continuous gradient measuring method, described Grad is concentration value or density value.
Preferably, in above-mentioned solid-state linear continuous gradient measuring method, described standard calibration curve is straight line.
Preferably, in above-mentioned solid-state linear continuous gradient measuring method, described coating on solid-state carrier has linearly
The known substance of continuous gradient is:
On solid-state carrier from low concentration to high concentration or low-density to high density coat described known substance.
Preferably, in above-mentioned solid-state linear continuous gradient measuring method, described standard calibration curve is positive number for slope
Straight line.
Preferably, in above-mentioned solid-state linear continuous gradient measuring method, described standard calibration curve is negative for slope
Straight line.
Preferably, in above-mentioned solid-state linear continuous gradient measuring method, described coating on solid-state carrier has linearly
The known substance of continuous gradient is:
On solid-state carrier from high concentration to low concentration or high density to low-density coat described known substance.
Preferably, in above-mentioned solid-state linear continuous gradient measuring method, described coating on solid-state carrier has linearly
The known substance of continuous gradient is:
On solid-state carrier from low concentration to high concentration or low-density to high density coat described known substance, and described
Know that concentration or the more high then final product concentrations of density of material are lower.
Preferably, in above-mentioned solid-state linear continuous gradient measuring method, described solid-state carrier be organic solid-state carrier or
Inorganic solid carrier.
The above-mentioned solid-state linear continuous gradient measuring method being provided by foregoing description, the present invention, due to first solid
On state carrier, coating has the known substance of LINEAR CONTINUOUS gradient, using described known substance as quantitative contrast foundation, then
Using each Grad of described known substance as independent variable, using the parameter value of each described gradient locations as dependent variable, make
Make standard calibration curve, then by unknown material to be checked put into described known substance identical condition system in and obtain described unknown
The parameter value of material to be checked, finally utilizes the parameter value of described unknown material to be checked and described standard calibration curve, described in calculating not
Know the Grad of material to be checked, measurement process is more rapid, more precisely and more convenience therefore, it is possible to make.
Brief description
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
Have technology description in required use accompanying drawing be briefly described it should be apparent that, drawings in the following description be only this
Inventive embodiment, for those of ordinary skill in the art, on the premise of not paying creative work, can also basis
The accompanying drawing providing obtains other accompanying drawings.
The schematic diagram of the first solid-state linear continuous gradient measuring method that Fig. 1 provides for the embodiment of the present application；
Fig. 2 is the instrumentation plan coating described known substance on solid-state carrier from low concentration to high concentration；
Fig. 3 is the instrumentation plan coating described known substance on solid-state carrier from high concentration to low concentration；
Fig. 4 is the inspection amount curve synoptic diagram of the inversely proportional slow fall of inspection amount curve.
Specific embodiment
The core concept of the present invention is to provide a kind of solid-state linear continuous gradient measuring method, can make measurement process more
Quickly, more precisely and more convenience, can be widely applied to various physical chemistry Qualitative and quantitative analysis, anti-including organic chemistry
Answer detection by quantitative, inorganic polymer composite flocculant detection by quantitative, biochemical reaction detection by quantitative, luminous energy chemical reaction detection by quantitative and life
Thing Immunoassay quantification detects.
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation description is it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of not making creative work
Embodiment, broadly falls into the scope of protection of the invention.
The first solid-state linear continuous gradient measuring method that the embodiment of the present application provides is as shown in figure 1, Fig. 1 is the application
The schematic diagram of the first solid-state linear continuous gradient measuring method that embodiment provides, the method comprises the steps:
S1: coating has the known substance of LINEAR CONTINUOUS gradient on solid-state carrier, using described known substance as quantitation
The foundation of contrast；
S2: using each Grad of described known substance as independent variable, the parameter value of each described gradient locations is made
For dependent variable, make standard calibration curve；
S3: by unknown material to be checked put into described known substance identical condition system in and obtain described unknown to be checked
The parameter value of material；
It should be noted that it is known that can take with unknown synchronization or synchronous reaction result in same effect system
Obtain conditional consistency, according to the linearity condition of known unlimited multiple spot, wherein continuous gradient concentration (numerical value) contrasts, you can synchronous
Try to achieve unknown test substance concentration values, reach the purpose of quick detection and treat to compare it might even be possible to be applied to macroscopic visual method
Survey the demarcation concentration values of material.
S4: using parameter value and the described standard calibration curve of described unknown material to be checked, calculate described unknown material to be checked
Grad.
It is known that LINEAR CONTINUOUS gradient concentration in this embodiment, by the corresponding unknown material of known linear continuous gradient concentration
Matter, acts in the same terms system, and known to generation one, unlimited linear multi correlates the numerical value of its known results, you can comparison
Draw the result of the relative science of unknown materials to be measured, this LINEAR CONTINUOUS gradient concentration determines straight line compared with traditional 2 points
Standard calibration curve method, the value of its test substance tried to achieve can be more accurate, and the verity closer to science.
Two or more effect substance system in it is also possible to by one of which matter utilization the present embodiment provide side
Case, by linear for this kind of material multiple spot gradient distribution coating, and another kind of unknown test substance effect, intercept relatively within the unit interval
Reaction density point, tries to achieve test substance concentration.
The above-mentioned solid-state linear continuous gradient measuring method being provided by foregoing description, the embodiment of the present application, due to
First on solid-state carrier, coating has the known substance of LINEAR CONTINUOUS gradient, using described known substance as quantitative contrast according to
According to, then using each Grad of described known substance as independent variable, using the parameter value of each described gradient locations as because
Variable, make standard calibration curve, then by unknown material to be checked put into described known substance identical condition system in and obtain
The parameter value of described unknown material to be checked, finally utilizes the parameter value of described unknown material to be checked and described standard calibration curve, meter
Calculate the Grad of described unknown material to be checked, measurement process is more rapid, more precisely and more convenience therefore, it is possible to make.
The second solid-state linear continuous gradient measuring method that the embodiment of the present application provides, is in the first solidus above-mentioned
On the basis of property continuous gradient measuring method, also include following technical characteristic:
Described Grad is concentration value or density value.
That is, both concentration value can have been measured using said method it is also possible to measurement density value, certainly can also measure
Other specification, is not limited herein.
The third solid-state linear continuous gradient measuring method that the embodiment of the present application provides, is in above-mentioned second solidus
On the basis of property continuous gradient measuring method, also include following technical characteristic:
Described standard calibration curve is straight line, and this straight line is easier to contrast and obtains result.
The 4th kind of solid-state linear continuous gradient measuring method that the embodiment of the present application provides, is in the third solidus above-mentioned
On the basis of property continuous gradient measuring method, also include following technical characteristic:
The described known substance that coating has LINEAR CONTINUOUS gradient on solid-state carrier is:
On solid-state carrier from low concentration to high concentration or low-density to high density coat described known substance.
Specifically, with reference to Fig. 2, Fig. 2 is the survey coating described known substance on solid-state carrier from low concentration to high concentration
Amount schematic diagram.
, this solid-state continuous gradient coating mainly comprises solid-state carrier known numeric value continuous gradient coating 1, inspection taking Fig. 2 as a example
Amount curve or can be straight line 11, known numeric value unit 12 and unknown numerical value test substance 13；It is known that numerical value continuous gradient applies
It is assumed that known numeric value continuous gradient coating 1 coating concentration numerical value is from 0 to 100 units, inspection amount curve 11 is fixed slope to layer 1, y
Axle relative concentration numerical value unit also it has been determined that so, can try to achieve arbitrarily unknown test substance relatively in this relative system
Concentration values 13；For example, ph indicator is coated on as continuous gradient coating no on dizzy dye paper according to known gradient concentration, passes through
The reaction color of this gradient figure layer is measured in known standard ph value buffer inspection, from shallow to deep to determine the inspection amount slope of curve, and confirms
Ph numerical value unit in the range of coating for the numerical value, that is, formula y=ax+b try to achieve；By unknown ph value determinand and continuous gradient coating
Reagent paper acts on, and compares the ph value that shade can try to achieve unknown determinand.
The 5th kind of solid-state linear continuous gradient measuring method that the embodiment of the present application provides, is in the third solidus above-mentioned
On the basis of property continuous gradient measuring method, also include following technical characteristic:
Described standard calibration curve is the straight line of positive number for slope, is described above, will not be described here.
The 6th kind of solid-state linear continuous gradient measuring method that the embodiment of the present application provides, is in the third solidus above-mentioned
On the basis of property continuous gradient measuring method, also include following technical characteristic:
Described standard calibration curve is the straight line of negative for slope.
The 7th kind of solid-state linear continuous gradient measuring method that the embodiment of the present application provides, is in above-mentioned 6th kind of solidus
On the basis of property continuous gradient measuring method, also include following technical characteristic:
The described known substance that coating has LINEAR CONTINUOUS gradient on solid-state carrier is:
On solid-state carrier from high concentration to low concentration or high density to low-density coat described known substance.
, Fig. 3 is that the measurement coating described known substance on solid-state carrier from high concentration to low concentration is illustrated taking Fig. 3 as a example
Figure, also can by the direction of continuous gradient coating be from the superficial to the deep transformed into by go deep into shallow applying, wherein known numeric value continuous gradient
Coating 2 assumes that inspection amount curve 21 is fixed slope, y-axis relative concentration by going deep into its concentration values of shallow coating from 0 to 100 units
Numerical value unit is also it has been determined that so, can try to achieve the relative concentration numerical value 23 of arbitrarily unknown test substance in this relative system；
For example, ph indicator is coated on no on dizzy dye paper as continuous gradient coating according to known gradient concentration, through known standard ph
The reaction color of this gradient figure layer is measured in value buffer inspection, from deep to shallow to determine the inspection amount slope of curve, and confirms numerical value in coating
In the range of the ph numerical value unit known, that is, formula y=-ax+b try to achieve；Unknown ph value determinand is made with continuous gradient coating reagent paper
With comparing the ph value that shade can try to achieve unknown determinand.The method depends on measurement purpose and measurement of species characteristic；Have
Its property of quantity of material to be measured is exactly just must to try to achieve relative value from high numerical value to the relative material effect of low numerical value a bit.
The 8th kind of solid-state linear continuous gradient measuring method that the embodiment of the present application provides, is in above-mentioned 6th kind of solidus
On the basis of property continuous gradient measuring method, also include following technical characteristic:
The described known substance that coating has LINEAR CONTINUOUS gradient on solid-state carrier is:
On solid-state carrier from low concentration to high concentration or low-density to high density coat described known substance, and described
Know that concentration or the more high then final product concentrations of density of material are lower.
Specifically, with reference to Fig. 4, Fig. 4 is the inspection amount curve synoptic diagram of the inversely proportional slow fall of inspection amount curve.Some homogenizing compete
Property immunoassay system, or chemical analysis system, compete reduction method using it, concentration value 3 is higher, response time shorter end-product
Concentration is lower, and such as concentration is the numerical value of corresponding unit 32 when 0 is 100, as can be seen that working as concentration on the inspection amount curve 31 obtaining
Numerical value for unit 33 corresponding when 50 is 50 units.Two or more materials participate in Competition, using this figure, known linear is terraced
Degree concentration value is it becomes possible to unknown materials concentration value in the reverse unit interval.
The 9th kind of solid-state linear continuous gradient measuring method that the embodiment of the present application provides, be above-mentioned the first to the 8th
In kind of solid-state linear continuous gradient measuring method on the basis of any one, also include following technical characteristic:
Described solid-state carrier is organic solid-state carrier or inorganic solid carrier, it can in addition contain be semisolid carrier, herein
Do not do any restriction.
It should be noted that said method application technology scope can include organic chemical analysis detection (inorganic chemical analysis
Detection, biochemical analysiss detection), biomedical check analyses detection, medical test and have examining in vitro of substrate or non-substrate
Disconnected reagent, medical test and the test of microorganism drug resistance Concentration Testing and gene detection test and micro-fluid chip detection examination
Test.
Described above to the disclosed embodiments, makes professional and technical personnel in the field be capable of or uses the present invention.
Multiple modifications to these embodiments will be apparent from for those skilled in the art, as defined herein
General Principle can be realized without departing from the spirit or scope of the present invention in other embodiments.Therefore, the present invention
It is not intended to be limited to the embodiments shown herein, and be to fit to and principles disclosed herein and features of novelty phase one
The scope the widest causing.
Claims (9)
1. a kind of linear continuous gradient measuring method of solid-state is it is characterised in that include:
On solid-state carrier, coating has the known substance of LINEAR CONTINUOUS gradient, using described known substance as quantitative contrast according to
According to；
Using each Grad of described known substance as independent variable, using the parameter value of each described gradient locations as because becoming
Amount, makes standard calibration curve；
By unknown material to be checked put into described known substance identical condition system in and obtain described unknown material to be checked
Parameter value；
Using parameter value and the described standard calibration curve of described unknown material to be checked, calculate the gradient of described unknown material to be checked
Value.
2. the linear continuous gradient measuring method of solid-state according to claim 1 is it is characterised in that described Grad is concentration
Value or density value.
3. the linear continuous gradient measuring method of solid-state according to claim 2 is it is characterised in that described standard calibration curve is
Straight line.
4. the linear continuous gradient measuring method of solid-state according to claim 3 it is characterised in that described on solid-state carrier
The known substance that coating has LINEAR CONTINUOUS gradient is:
On solid-state carrier from low concentration to high concentration or low-density to high density coat described known substance.
5. the linear continuous gradient measuring method of solid-state according to claim 3 is it is characterised in that described standard calibration curve is
Slope is the straight line of positive number.
6. the linear continuous gradient measuring method of solid-state according to claim 3 it is characterised in that
Described standard calibration curve is the straight line of negative for slope.
7. the linear continuous gradient measuring method of solid-state according to claim 6 it is characterised in that described on solid-state carrier
The known substance that coating has LINEAR CONTINUOUS gradient is:
On solid-state carrier from high concentration to low concentration or high density to low-density coat described known substance.
8. the linear continuous gradient measuring method of solid-state according to claim 6 it is characterised in that described on solid-state carrier
The known substance that coating has LINEAR CONTINUOUS gradient is:
On solid-state carrier from low concentration to high concentration or low-density coats described known substance, and described known substance to high density
The concentration of matter or the more high then final product concentrations of density are lower.
9. the linear continuous gradient measuring method of the solid-state according to any one of claim 1-8 is it is characterised in that described solid-state
Carrier is organic solid-state carrier or inorganic solid carrier.
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Citations (4)
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|---|---|---|---|---|
| CN102288586A (en) * | 2011-05-17 | 2011-12-21 | 首都医科大学附属北京友谊医院 | Method for determining minimal inhibitory concentration of drug |
| CN102735846A (en) * | 2012-06-15 | 2012-10-17 | 河南生生医疗器械有限公司 | Chemiluminescence immunodetection kit and detection method for ovarian cancer tumor marker HE4 |
| CN103575894A (en) * | 2013-11-07 | 2014-02-12 | 南京祥中生物科技有限公司 | Detection method for visible biological chip |
| CN105255990A (en) * | 2015-11-20 | 2016-01-20 | 中国药科大学 | Leukotrienes B4 receptor 1 antagonist medicine screening model based on fluorescence resonance energy transfer technology |
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2016
- 2016-08-25 CN CN201610726776.0A patent/CN106370654A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102288586A (en) * | 2011-05-17 | 2011-12-21 | 首都医科大学附属北京友谊医院 | Method for determining minimal inhibitory concentration of drug |
| CN102735846A (en) * | 2012-06-15 | 2012-10-17 | 河南生生医疗器械有限公司 | Chemiluminescence immunodetection kit and detection method for ovarian cancer tumor marker HE4 |
| CN103575894A (en) * | 2013-11-07 | 2014-02-12 | 南京祥中生物科技有限公司 | Detection method for visible biological chip |
| CN105255990A (en) * | 2015-11-20 | 2016-01-20 | 中国药科大学 | Leukotrienes B4 receptor 1 antagonist medicine screening model based on fluorescence resonance energy transfer technology |
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