CN109596556A - The method of Pb In Exhausted Water ion concentration is quickly and easily measured using Quercetin - Google Patents
The method of Pb In Exhausted Water ion concentration is quickly and easily measured using Quercetin Download PDFInfo
- Publication number
- CN109596556A CN109596556A CN201811525819.4A CN201811525819A CN109596556A CN 109596556 A CN109596556 A CN 109596556A CN 201811525819 A CN201811525819 A CN 201811525819A CN 109596556 A CN109596556 A CN 109596556A
- Authority
- CN
- China
- Prior art keywords
- solution
- mol
- lead
- concentration
- quercetin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 title claims abstract description 54
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 title claims abstract description 27
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 title claims abstract description 27
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 title claims abstract description 27
- 229960001285 quercetin Drugs 0.000 title claims abstract description 27
- 235000005875 quercetin Nutrition 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 16
- RECVMTHOQWMYFX-UHFFFAOYSA-N oxygen(1+) dihydride Chemical compound [OH2+] RECVMTHOQWMYFX-UHFFFAOYSA-N 0.000 title claims abstract description 9
- 239000000243 solution Substances 0.000 claims abstract description 46
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 31
- 238000002835 absorbance Methods 0.000 claims abstract description 25
- 238000006243 chemical reaction Methods 0.000 claims abstract description 24
- 229940046892 lead acetate Drugs 0.000 claims abstract description 21
- RVPVRDXYQKGNMQ-UHFFFAOYSA-N lead(2+) Chemical compound [Pb+2] RVPVRDXYQKGNMQ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000012086 standard solution Substances 0.000 claims abstract description 18
- PNZVFASWDSMJER-UHFFFAOYSA-N acetic acid;lead Chemical compound [Pb].CC(O)=O PNZVFASWDSMJER-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000001228 spectrum Methods 0.000 claims abstract description 10
- 230000003287 optical effect Effects 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000012153 distilled water Substances 0.000 claims abstract description 6
- 230000035484 reaction time Effects 0.000 claims description 3
- 239000012088 reference solution Substances 0.000 claims description 3
- 238000005259 measurement Methods 0.000 abstract description 5
- 235000019441 ethanol Nutrition 0.000 description 8
- 238000002798 spectrophotometry method Methods 0.000 description 4
- 235000004237 Crocus Nutrition 0.000 description 3
- 241000596148 Crocus Species 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000000376 reactant Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000003968 anodic stripping voltammetry Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000007096 poisonous effect Effects 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The method that Pb In Exhausted Water ion concentration is quickly and easily measured using Quercetin, is related to the measurement of lead ion in solution, comprising: (1) determination of characteristic wavelength；I.e. by after Quercetin ethanol solution and lead acetate solution reaction in 200-800nm scanning optical spectrum；It then proceedes to take multiple scan determining characteristic wavelength in 200-800nm scanning optical spectrum；(2) standard curve is drawn under optimum reaction condition；(3) by lead acetate solution allocation at five standard solution of various concentration；(4) the Quercetin solution of equivalent is added in acetic acid lead solution, the absorbance of ultraviolet specrophotometer measurement lead ion is used under optimum reaction condition, it takes sample to be tested in cuvette, is returned to zero using distilled water as blank, measure absorbance in the certain wave strong point that step (1) determines；Using lead acetate concentration of standard solution as X-axis, using solution absorbance after reaction as Y-axis, standard curve is drawn.The present invention has the advantages that quick, simple, economical.
Description
Technical field
It is specifically a kind of quickly and easily to measure Pb In Exhausted Water using Quercetin the present invention relates to the measurement of lead ion in solution
The method of ion concentration.
Background technique
Lead is a kind of accumulation property poisonous substance, can be accumulated in human body and animal tissue, and lead is for the very harmful of human body, master
Wanting poisonous effect is to lead to anaemia, nervous function imbalance and injury of kidney etc..The present invention is anti-in conjunction with lead ion using Quercetin
Phenomenon is answered, lead ion is measured, applied to the detection of Pb In Exhausted Water ion, Quercetin (C16H10O7) it is to be mentioned in natural plants
A kind of compound taken is yellow needles powder, can change colour in conjunction with metal ion, and Quercetin is anti-with lead ion
Crocus is presented in Ying Hou, reactant, if Quercetin reaction is added in water sample is presented crocus, illustrates there is lead ion in water sample
In the presence of being detected using this phenomenon to the presence of industrial wastewater ion.
Currently, the detection method of lead ion has very much, there are atomic absorption spectrography (AAS), anodic stripping voltammetry, spectrophotometric
Method etc., atomic absorption spectrography (AAS) precision is poorer than spectrophotometry, and the range of linearity of standard working curve is narrow, anodic stripping voltammetry
Method is complicated for operation, at high cost, and in comparison, spectrophotometry is because its is low in cost, easy to operate, finding speed is fast, accurate
Spend it is higher, have a wide range of application, it is easy to spread using and become detection the most common analyzing detecting method of lead, therefore the present invention use
Spectrophotometric Determination of Lead ion.
Summary of the invention
It is an object of that present invention to provide a kind of new methods for quickly, simply measuring Pb In Exhausted Water ion concentration.
A kind of Pb In Exhausted Water ion concentration measuring method, comprising the following steps:
(1) determination of characteristic wavelength；
(2) optimize reaction condition；
(3) various concentration solution is configured；
(4) standard curve is drawn.
In method of the invention, step (1) be by after Quercetin ethanol solution and lead acetate solution reaction in 200-800nm
Scanning optical spectrum changes acetic acid lead concentration, then proceedes to take multiple scan in 200-800nm scanning optical spectrum, it is therefore an objective to determine
Its characteristic wavelength.Step (2) is to draw standard curve with this condition to find optimum reaction condition.
Lead acetate solution allocation it is dense have been prepared 5 gradients as standard solution at the solution of various concentration by step (3)
The lead acetate standard solution of degree is 1 × 10-4mol/L、2×10-4mol/L、3×10-4mol/L、4×10-4mol/L、5×10- 4mol/L
Wherein step (4) be by step (3) in the acetic acid lead solution of various concentration be added equivalent Quercetin solution, after reaction
Solution uses ultraviolet specrophotometer to measure its absorbance at optimum conditions, takes sample to be tested in right amount in cuvette, to steam
Distilled water returns to zero as blank, measures absorbance in the certain wave strong point that (1) step determines；It is X with lead acetate concentration of standard solution
Axis draws standard curve using solution absorbance after reaction as Y-axis.
The principle of the method for the present invention: after Quercetin is reacted with lead ion, crocus is presented in reactant, and solution is purple after reaction
Outer visible spectrophotometer is measured.Experimental studies have found that within the scope of a certain concentration, lead ion solution and itself and Quercetin
The absorbance that solution measures after reaction can show good concentration-absorbance curve.Within this range, it measures with can be convenient
Lead ion content in solution.
Compared with existing measuring method, the present invention has the advantages that quick, simple, economical.Lead ion is being determined in spy
After determining the characteristic wavelength and standard curve in concentration Quercetin ethanol solution, it is only necessary to measure sample to be tested in characteristic wave strong point
The concentration of lead ion can be calculated in absorbance.
Detailed description of the invention
Quercetin ethanol solution and lead ion reactant full wavelength scanner map Fig. 1 of the invention.
The standard curve of the obtained lead ion of Fig. 2 embodiment 1 measurement.
The standard curve of the obtained lead ion of Fig. 3 embodiment 2 measurement.
Specific embodiment
1, it determines characteristic wavelength: being 10 to ultimate density-5Lead acetate is added in the Quercetin ethanol solution of mol/L makes it
Final concentration of 10-4Then mol/L determines its characteristic wavelength in 200-800nm scanning optical spectrum；Change acetic acid lead concentration 10-3mol/
L、10-2Mol/L finally determines that its characteristic wavelength is 449nm then in 200-800nm scanning optical spectrum.
2, Specification Curve of Increasing:
The lead acetate standard solution of 0.1mol/L is configured, the lead acetate standard solution of 0.1mol/L is then diluted to 1 respectively ×
10-4mol/L、2×10-4mol/L、3×10-4mol/L、4×10-4mol/L、5×10-4Quercetin ethanol solution is added in mol/L
As concentration be 4 × 10-5 mol/L mixed liquor, pH be 7, the reaction time be 3 minutes, 90 DEG C of temperature under conditions of, in
Absorbance is measured using distilled water as reference solution at 449nm, using lead acetate concentration of standard solution as X-axis, with solution after reaction
Absorbance is Y-axis, draws standard curve；The regression equation of standard curve is calculated with least square method:
Y=aX+b
Y --- solution absorbance after reaction；
X --- lead acetate concentration of standard solution, unit are 10-4mol/L；
The slope of a --- regression equation；
The intercept of b --- regression equation；
The lead ion solution of various concentration carries out absorbance experiment after chromogenic reaction, and experimental data see the table below 1:
Absorbance of the 1 various concentration acetic acid lead solution of table in Quercetin ethanol solution
Data according to table 1: indicating absorbance with y, indicates concentration with x, through calculating it can be concluded that the two there are following
Linear relationship y=0.0113x+0.2611, being computed its related coefficient square is 0.9947.
Embodiment 2
1. determining characteristic wavelength: with embodiment 1
2. Specification Curve of Increasing: the lead acetate standard solution of 0.1mol/L is configured, it is then that the lead acetate standard of 0.1mol/L is molten
Liquid is diluted to 1 × 10 respectively-3mol/L、2×10-4mol/L、3×10-3mol/L、4×10-3It is molten that Quercetin ethyl alcohol is added in mol/L
Liquid makes its ultimate density 4 × 10-5Mol/L is reacted 3 minutes under conditions of 90 DEG C of temperature, is made at 449nm with distilled water
Absorbance is measured for reference solution, using lead acetate concentration of standard solution as X-axis, using solution absorbance after reaction as Y-axis, draws mark
Directrix curve.
Absorbance of the 2 various concentration acetic acid lead solution of table in Quercetin ethanol solution
According to the data measured: indicating absorbance with y, indicate concentration with x, by calculating it can be concluded that the two exists with lower linear
Relationship y=0.0089x+0.254, being computed its related coefficient square is 0.99735.
Claims (3)
1. quickly and easily measuring the method for Pb In Exhausted Water ion concentration using Quercetin, comprising the following steps:
(1) determination of characteristic wavelength；Light will be scanned in 200-800nm after Quercetin ethanol solution and lead acetate solution reaction
Spectrum；Change acetic acid lead concentration, then proceedes to take multiple scan determining lead ion characteristic wave in 200-800nm scanning optical spectrum
It is long；
(2) optimize reaction condition, draw standard curve under the optimum reaction condition determined；
(3) by lead acetate solution allocation at five standard solution of various concentration, five standard solution are respectively 1 × 10-4mol/
L、2×10-4mol/L、3×10-4mol/L、4×10-4mol/L、5×10-4Five strength solutions of mol/L；
(4) the Quercetin solution of equivalent is added in acetic acid lead solution, ultraviolet specrophotometer is used under optimum reaction condition
The absorbance for measuring lead ion, takes sample to be tested in cuvette, is returned to zero using distilled water as blank, is determined in step (1)
Certain wave strong point measures absorbance；Using lead acetate concentration of standard solution as X-axis, using solution absorbance after reaction as Y-axis, mark is drawn
Directrix curve.
2. the method for quickly and easily measuring Pb In Exhausted Water ion concentration using Quercetin according to claim 1, it is characterized in that
The optimum reaction condition is that pH is 7, and temperature is 90 DEG C, and the reaction time is 3 minutes.
3. the method for quickly and easily measuring Pb In Exhausted Water ion concentration using Quercetin according to claim 1, it is characterized in that
It is 10 to ultimate density-5It is 10 that acetic acid lead solution is added in the Quercetin ethanol solution of mol/L to become concentration-4The mixing of mol/L
Then solution determines characteristic wavelength in 200-800nm scanning optical spectrum；Change acetic acid lead concentration 10-3mol/L、10-2Mol/L, so
It is final to determine that characteristic wavelength is 449nm afterwards in 200-800nm scanning optical spectrum；The lead acetate standard solution of 0.1mol/L is configured, so
The lead acetate standard solution of 0.1mol/L is diluted to 1 × 10 respectively afterwards-4mol/L、2×10-4mol/L、3×10-4mol/L、4
×10-4mol/L、5×10-4Mol/L, Quercetin ethanol solution, which is added, becomes the mixed liquor that concentration is 4 × 10-5 mol/L,
PH is 7, the reaction time be under conditions of 3 minutes, 90 DEG C of temperature measured at 449nm using distilled water as reference solution lead from
Sub- absorbance, using solution absorbance after reaction as Y-axis, draws standard curve using lead acetate concentration of standard solution as X-axis；With most
Small square law calculates the regression equation of standard curve:
Y=aX+b
Y --- solution absorbance after reaction；
X --- lead acetate concentration of standard solution, unit are 10-4mol/L；
The slope of a --- regression equation；
The intercept of b --- regression equation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811525819.4A CN109596556A (en) | 2018-12-13 | 2018-12-13 | The method of Pb In Exhausted Water ion concentration is quickly and easily measured using Quercetin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811525819.4A CN109596556A (en) | 2018-12-13 | 2018-12-13 | The method of Pb In Exhausted Water ion concentration is quickly and easily measured using Quercetin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109596556A true CN109596556A (en) | 2019-04-09 |
Family
ID=65961952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811525819.4A Pending CN109596556A (en) | 2018-12-13 | 2018-12-13 | The method of Pb In Exhausted Water ion concentration is quickly and easily measured using Quercetin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109596556A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115436309A (en) * | 2022-08-29 | 2022-12-06 | 天津大学 | Ultraviolet detection method for quickly and accurately measuring lead concentration |
| CN115950845A (en) * | 2023-03-09 | 2023-04-11 | 国能龙源环保有限公司 | Method for determining tetravalent vanadium content in pickle liquor of waste denitration catalyst |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105548056A (en) * | 2015-12-22 | 2016-05-04 | 天津凯英科技发展有限公司 | Method for detecting contents of metal lead ions in samples |
| CN105851781A (en) * | 2015-04-10 | 2016-08-17 | 李�杰 | Stabilizing agent capable of realizing degradation, detoxication and harmless treatment of heavy metals in food, foodstuffs and traditional Chinese medicines and preparation method thereof |
-
2018
- 2018-12-13 CN CN201811525819.4A patent/CN109596556A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105851781A (en) * | 2015-04-10 | 2016-08-17 | 李�杰 | Stabilizing agent capable of realizing degradation, detoxication and harmless treatment of heavy metals in food, foodstuffs and traditional Chinese medicines and preparation method thereof |
| CN105548056A (en) * | 2015-12-22 | 2016-05-04 | 天津凯英科技发展有限公司 | Method for detecting contents of metal lead ions in samples |
Non-Patent Citations (2)
| Title |
|---|
| J. P. CORNARD,ET AL: "Computational and Spectroscopic Characterization of the Molecular and Electronic Structure of the Pb(II)-Quercetin Complex", 《J. PHYS. CHEM. A》 * |
| 方月娟 等: "分光光度法测定天然产物提取物对铅离子螯合能力的研究", 《中华中医药学刊》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115436309A (en) * | 2022-08-29 | 2022-12-06 | 天津大学 | Ultraviolet detection method for quickly and accurately measuring lead concentration |
| CN115950845A (en) * | 2023-03-09 | 2023-04-11 | 国能龙源环保有限公司 | Method for determining tetravalent vanadium content in pickle liquor of waste denitration catalyst |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100498287C (en) | Nano argentum spectrophotometry for detecting hydroxy free radical | |
| CN107421899A (en) | Determine the detection method of water quality aniline, formaldehyde simultaneously based on ultraviolet-visible absorption spectroscopy | |
| CN108037084B (en) | Anti-interference measuring method suitable for photometric principle water quality automatic analyzer | |
| CN109596556A (en) | The method of Pb In Exhausted Water ion concentration is quickly and easily measured using Quercetin | |
| CN111189800A (en) | Method for determining content and concentration of vitronectin by using refractive index test | |
| CN207816810U (en) | The calibrating installation of Long path differential optical absorption spectroscopy air quality monitor | |
| RU2533322C1 (en) | Method of determining total content of phenoles in waters | |
| RU2391659C1 (en) | Method of detecting silver using polymethacrylate matrix | |
| CN101498670B (en) | Method for measuring nitrogen oxide in cigarette side-stream smoke | |
| CN104330383A (en) | Method for analyzing valence of edible oleic acid by using FTIR method | |
| CN102798606A (en) | Method for rapidly measuring preparation proportion of cigarette balsam to cigarette material liquid | |
| CN108802083A (en) | A kind of method of sulphur, chlorinity in measurement triphenylphosphine | |
| CN103398966A (en) | Method for detecting TMC concentration in organic solution by using spectrometer | |
| Fedosov et al. | Microtitration of free fatty acids in oil and biodiesel samples using absorbance and/or fluorescence of pyranine | |
| CN103983624A (en) | Fluorescence method for determining the chemical oxygen demand | |
| CN108508008A (en) | The test paper of ammonia and its preparation and assay method in a kind of quantitative determination air | |
| Purbaningtias et al. | The performance study of a single and double beam UV-Vis spectrophotometer on nitrite determination in groundwater | |
| CN108414461A (en) | A method of improving silicate component measuring accuracy | |
| CN117191728B (en) | Method for measuring multi-component concentration based on ultraviolet-visible absorption spectrum and application | |
| Mansourian et al. | Simultaneous determination of two alcohols and one anionic surfactant in commercial liquid cleaners using partial least square regression by Fourier transform infrared spectrometry | |
| Crisponi et al. | Evaluation of a Fibre Optic Device in Solution Equilibria Studies. Application to 3‐Hydroxybenzoic Acid Ionization | |
| Shamsipur et al. | Development of a PVC-membrane ion-selective bulk optode, for UO 2 2+ ion, based on tri-n-octylphosphine oxide and dibenzoylmethane | |
| KR101204561B1 (en) | Nitrate concentration measuring method | |
| Burgess et al. | A kinetic method for the determination of arsenic (III), antimony (III) and ascorbic acid | |
| Zhao et al. | A new spectrum technique based on direct detection of light intensity absorbed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190409 |
|
| WD01 | Invention patent application deemed withdrawn after publication |