CN106370635B - Method, equipment and system for quantitatively detecting content of polar components in edible oil - Google Patents

Method, equipment and system for quantitatively detecting content of polar components in edible oil Download PDF

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CN106370635B
CN106370635B CN201610784248.0A CN201610784248A CN106370635B CN 106370635 B CN106370635 B CN 106370635B CN 201610784248 A CN201610784248 A CN 201610784248A CN 106370635 B CN106370635 B CN 106370635B
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edible oil
content
signal
digital signal
polar components
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CN106370635A (en
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汤新华
陈秀琼
林晓丽
蓝惠英
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XIAMEN STANDARDS SCIENTIFIC INSTRUMENT CO LTD
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XIAMEN STANDARDS SCIENTIFIC INSTRUMENT CO LTD
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters

Abstract

Discloses a method for quantitatively detecting polar components in edible oil, which comprises the following steps: exciting the edible oil with light of a specific wavelength; collecting a fluorescent signal emitted by the edible oil under the excitation of the light with the specific wavelength; obtaining a digital signal indicative of the intensity of the fluorescent signal; and calculating the content of the polar components of the edible oil corresponding to the digital signal representing the fluorescence signal intensity based on the linear relation between the predetermined digital signal and the content of the polar components of the edible oil. Also discloses a device and a system for quantitatively detecting the content of the polar component in the edible oil by the method.

Description

Method, equipment and system for quantitatively detecting content of polar components in edible oil
Technical Field
The invention belongs to the technical field of food detection, and particularly relates to a method and related equipment for quantitatively detecting the content of polar components in edible oil.
Background
The edible oil is the most common seasoning used by residents, is also a medium for heating raw materials, and has the functions of seasoning and heat transfer. The edible oil is one of important nutrient substances required by human body, and is an indispensable necessity in our daily life. However, in recent years, the quality of edible oil is a constant threat to people's health, and even reports of returning illegal cooking oil to dining tables are frequent: some bad vendors simply treat the reused edible oil and return the oil to the dining table again, and the reused edible oil poses a serious threat to human health.
The polar components mean components produced by the degradation of edible oil under frying process conditions, undergoing thermal oxidation reaction, thermal polymerization reaction, thermal oxidation polymerization reaction, thermal cracking reaction and hydrolysis reaction, and having a polarity greater than that of normal vegetable oil molecules (triglycerides), including thermal oxidation products of triglycerides (triglycerides containing ketone groups, hydroxyl groups, peroxide groups and carboxyl groups), thermal polymerization products, thermal oxidation polymerization products, hydrolysis products (free fatty acids, monoglycerides and diglycerides) and the like.
The phenomenon of higher polar components occurs in edible oils that are used repeatedly. The 'sanitary standard in the frying process of edible vegetable oil' issued and implemented in China clearly stipulates that the content of polar components in the edible oil cannot exceed 27 percent. Therefore, the quality of the edible oil can be measured by quantitatively detecting the polar components in the edible oil so as to guide the use duration and the use times of the edible oil in the frying process.
However, the column chromatography adopted in the current national standard has long detection time, needs to consume a large amount of organic reagents, needs to be operated by professional personnel, requires complicated detection equipment, and is not beneficial to wide popularization and application. For this reason, researchers use colorimetric methods to measure polar components, but the methods require tedious pre-processing of samples, and the detection results only include qualitative determination of samples without accurate quantitative analysis, so that the methods are not significant for the practical needs of quantitative measurement of polar components.
Therefore, there is still a need to develop a simple and easy-to-use method for quantitatively detecting polar components in edible oil.
Disclosure of Invention
One object of the present invention is to provide a method for quantitatively detecting the content of polar components in edible oil, which comprises the following steps: exciting the edible oil with light of a specific wavelength; collecting a fluorescent signal emitted by the edible oil under the excitation of the light with the specific wavelength; obtaining a digital signal indicative of the intensity of the fluorescent signal; and calculating the content of the polar components of the edible oil corresponding to the digital signal representing the intensity of the fluorescence signal based on the linear relation between the predetermined digital signal and the content of the polar components of the edible oil.
Another object of the present invention is to provide an apparatus for quantitatively detecting the content of polar components in edible oil, which comprises: the excitation light source is used for emitting light with a specific wavelength to the edible oil; a fluorescent signal receiving part for receiving a fluorescent signal emitted by the edible oil under the excitation of the light with the specific wavelength; a signal processing section for processing the fluorescence signal to obtain a digital signal representing an intensity of the fluorescence signal; and the polar component content determining part is used for solving the content of the polar components of the edible oil corresponding to the digital signal representing the intensity of the fluorescent signal based on the linear relation between the predetermined digital signal and the content of the polar components of the edible oil.
Still another object of the present invention is to provide a system for quantitatively detecting the content of polar components in edible oil, which comprises: the device for quantitatively detecting the content of the polar components of the edible oil is used as a detection induction end; the data control terminal is communicated with the detection induction end in a wireless communication mode; and the data control terminal receives the detection result of the content of the polar components of the edible oil from the detection induction terminal in a wireless communication mode and displays the detection result of the content of the polar components of the edible oil.
Drawings
Fig. 1 is a schematic diagram of a method for quantitatively detecting the content of polar components in edible oil according to an embodiment of the present invention.
FIG. 2 is a schematic diagram of a fluorescence detector according to an embodiment of the present invention.
FIG. 3 is an enlarged partial view of the fluorescence emitting and receiving portion of the fluorescence detector of FIG. 2.
FIG. 4 is a signal processing schematic of fluorescence signal detection according to an embodiment of the present invention.
Fig. 5 is a circuit diagram of an excitation light source module of the edible oil polar component content detection system according to the embodiment of the invention.
Fig. 6 is a circuit diagram of a photoelectric conversion and I/V conversion module of an edible oil polar component content detection system according to an embodiment of the invention.
FIG. 7 is a schematic diagram of a general detection process for the content of polar components in edible oil according to the present invention.
Detailed Description
In order to make the technical solutions of the present invention better understood, the present invention is further described below with reference to the accompanying drawings and the detailed description.
Fig. 1 shows a flow chart of a method for quantitatively detecting the content of polar components in edible oil according to the present invention. As shown in fig. 1, the method includes, in step ST1, exciting the edible oil to be tested with light of a specific wavelength (for example, light of 450nm) based on the principle that some substances such as edible oil can generate fluorescence under excitation of excitation light of a specific wavelength; in step ST2, collecting a fluorescent signal emitted from the edible oil under excitation of the light of the specific wavelength; in step ST3, a digital signal representing the intensity of the fluorescence signal is obtained (for example, the peak area of the fluorescence signal (i.e., the area covered by the portion whose value of the vertical axis is greater than the baseline) in the fluorescence spectrum curve (whose horizontal axis is the wavelength and whose value of the vertical axis is the intensity of the fluorescence signal) is converted into a corresponding digital signal by a series of conversions, and the digital signal is the digital signal representing the intensity of the fluorescence signal; thereafter, in step ST4, the content of the polar component of the edible oil is found based on the linear relationship between the digital signal and the content of the polar component of the edible oil.
Determination of a correlation Linear relationship
In the method for quantitatively detecting the content of the polar components of the edible oil, the content of the polar components of the edible oil corresponding to the obtained digital signal is finally obtained by utilizing the linear relation between the predetermined digital signal representing the fluorescence intensity and the content of the polar components of the edible oil. To determine the linear relationship, in the present invention, a small handheld fluorescence detector is first constructed, and the fluorescence detector emits light with a specific wavelength (450nm) to excite the edible oil to be measured, and the fluorescence detector is used to collect the fluorescence signal emitted by the edible oil to be measured under the excitation of the light with the specific wavelength, and then the collected fluorescence signal is converted into an alternating current signal by a photoelectric converter, the analog alternating current signal is converted into an analog direct current signal by an alternating current-direct current converter, and the analog direct current signal is converted into a digital signal representing the fluorescence signal intensity of the edible oil to be measured by an analog-to-digital converter. The above process is carried out on a plurality of edible oil samples to be measured, and a plurality of digital signals are obtained. Meanwhile, detecting the multiple edible oil samples to be detected by adopting a national standard method to obtain multiple polar component content detection values. And then, taking the digital signal representing the fluorescence signal intensity of the edible oil to be detected as an X value, and taking a corresponding polar component content detection value obtained by detecting the edible oil to be detected by a national standard method such as the aforementioned column chromatography as a Y value, and performing curve fitting to obtain a functional relation between the Y value and the X value. Because when the content of the polar component of the edible oil is in the range of 5-50% (in practical application, the range can meet the requirement of detecting the content of the polar component of the edible oil), the fluorescence signal intensity and the content of the polar component have a good linear relation. Therefore, by linear fitting, a linear relationship between the Y value and the X value can be obtained as Y ═ 0.062X-14.57.
FIG. 2 shows the fluorescence detector described above. As shown in fig. 2, the fluorescence detector mainly includes an upper cover 1 of the instrument housing, a lower cover 2 of the instrument housing, a screen 3, a buzzer 4, a battery 5, a battery cover 6, a metal probe 7, a detector 8, a focusing device 9, a glass sheet 10, a glass sheet 11, an excitation light source 12, a decoration 13, a key 14, a circuit board 15, a screen decoration 16, and the like.
FIG. 3 is an enlarged partial view of the fluorescence emitting and receiving portion of the fluorescence detector of FIG. 2. As shown in fig. 3, the metal probe 7 of the fluorescence detector is inserted into a container containing sample oil, the light of 450nm emitted from the excitation light source 12 of the fluorescence detector is transmitted through the glass sheet 11, horizontally irradiates in the sample oil to excite the sample oil to emit a fluorescence signal, the fluorescence signal is transmitted through the glass sheet 10 in the vertical direction, passes through the focusing device 9, and is received by the detector 8. The received fluorescent signal is then converted into an electrical signal and further processed and analyzed to obtain the polar component content in the sample oil, and the polar component content is then sent to a data processing terminal for display, so that a user can judge whether the sample oil is qualified or not based on the polar component content. In addition, the method can be used for producing a composite material
According to the invention, the content detection system for polar components in edible oil
FIG. 4 is a signal processing schematic of fluorescence signal detection according to an embodiment of the present invention. Among them, the photoelectric sensor, the LED driving circuit, the LED lamp, the I/V converter, the amplifier, the ac/dc converter, the analog-to-digital converter, and the STM32 processor in fig. 4 are provided in the above-mentioned fluorescent detector, i.e., a so-called detection sensing terminal, and the data storage device, the data transmission device, the data printing device, and the like are provided in a so-called data control terminal such as a mobile phone or a tablet computer. As shown in fig. 4, the working flow of the detection sensing end of the edible oil polar component content detection system includes: the microprocessor STM32 generates an alternating current driving signal with specific frequency to drive the LED to generate exciting light through the LED driving circuit of the detection induction end, and the alternating current driving signal is used for exciting the edible oil to be detected to send out a fluorescence signal which is converted into an alternating current signal through the photoelectric sensor, the alternating current signal is converted into an alternating voltage signal by the I/V converter, the alternating voltage signal is amplified by the amplifier, then the alternating voltage signal is converted into a direct current signal through the alternating current-direct current converter, and the direct current signal is sampled by the digital-analog converter to be converted into a digital signal, so that the fluorescent signal and the intensity of the edible oil are captured. The digital signal is input into a microprocessor STM32, and the latter can obtain the polar component content value of the edible oil to be detected according to the linear relation between the digital signal stored in the detection induction end and the polar component content of the edible oil, and the polar component content value is used as a final detection result and is sent to a data control terminal through Bluetooth for example, and is displayed on the data control terminal and is correspondingly stored.
The data control terminal and the detection sensing terminal can communicate with each other through an application program (app) on the data control terminal through a Bluetooth communication module, for example, the detection sensing terminal can transmit the content of the polar components of the detected edible oil to the app on the data control terminal through the Bluetooth communication module, and the app can display the content of the polar components on the data control terminal so as to enable a user to judge whether the quality of the edible oil is qualified. Furthermore, control of the detection sensing terminal and subsequent data storage, transmission and tracking can be achieved through an application program (app). For example, those skilled in the art will appreciate that the app can be installed on a data control terminal, such as a cell phone or tablet computer, for user operation, which is connected to a detection sensing terminal, such as the fluorescence detector described above, via, for example, a bluetooth communication module. The successfully connected app is approximately equal to a central console, and a user can click detection, data query and other function keys on the app to control the operation of the fluorescence detector. With the app, the user can also perform additional functions such as data storage, data transmission, data printing, data editing, and the like, in addition to detection.
Some of the components of the fluorescence detector according to the invention will be described in more detail below with reference to fig. 5 and 6.
Fig. 5 illustrates an excitation light source module that can be used in embodiments of the invention. As shown in fig. 5, the excitation light source module is composed of a constant current LED driving chip, an adjustable resistor R1, and a light emitting diode D1 that emits light with a fixed wavelength of 450nm, for example; the pulse width modulation signal is loaded on the enable pin (EN) of the driving chip, so that simple brightness control (dimming function) can be realized, namely the duty ratio of the pulse width modulation signal is changed to adjust the size of the output current. The highest driving current of each LED can reach 100mA, the current matching precision reaches 0.3%, and compared with the traditional method of driving the LEDs through the voltage of series resistors, the LED driving circuit has more stable brightness and higher efficiency. The light source has the characteristics of low power consumption, high efficiency, high stability and the like. Although the embodiment of fig. 5 is to emit light with a wavelength of 450nm by using LEDs, the embodiment is not limited to this, and other types of light sources, such as deuterium lamps and xenon lamps, may be used to emit light with a wavelength of 450 nm.
Fig. 6 shows a photoelectric conversion, I/V conversion module that can be used in an embodiment of the present invention. As shown in fig. 6, the module is composed of a low-bias high-input-impedance high-gain precision operational amplifier U3, a photodiode D2, resistors R2, R3 and R4; after the photodiode D2 has completed the photoelectric conversion of the fluorescent signal, the resulting current signal is converted into a corresponding voltage signal by an I/V conversion module (current/voltage converter), which has different amplification factors for the ac and dc signals, specifically, the dc amplification factor VDC=IindX (R2+ R4); and its AC amplification factor VAC=IinaX R2(1+ R4/R3), wherein IindRepresenting a direct input current, IinaRepresenting the ac input current.
Those skilled in the art can understand that after the received fluorescent signal is subjected to the aforementioned photoelectric conversion and I/V conversion, a conventional amplifier, an ac/dc converter and a digital-to-analog converter can be selected according to actual needs to continue signal processing until a digital signal capable of representing the intensity of the received fluorescent signal is obtained and sent to an STM32 processor as an input to obtain the polar component content of the edible oil to be measured.
Before detection, the sample does not need to be pretreated or any reagent is added, the whole detection process only needs about 10s, and all non-professionals can easily control the operation of the instrument, so the oil quality monitoring instrument can be widely applied to oil quality monitoring in real life.
General procedure for detecting content of polar components in edible oil
The following steps can be generally performed in sequence according to the quantitative determination of the polar component content in the edible oil of the present invention: the first step is sampling, and the concrete operation is to shake up an edible oil sample to be detected, take about 20mL of the sample to pour into a disposable sample cup to be detected, and at the moment, place the oil sample in a shading device (such as a shading cup or a shading black cover); the second step is detection, specifically, the fluorescence detector is vertically inserted into a sample cup, a detection button of the instrument is clicked, the detection is started, 5-10s of detection results of the polar component content given by the instrument are waited, the detection is finished, and the detection results can be sent to a data control terminal such as a mobile phone or a tablet personal computer through a Bluetooth communication module and displayed on the data control terminal; the third step is cleaning, which is specifically performed by cleaning the fluorescence detector with a detergent or wiping the fluorescence detector with a paper towel.
The invention has the following characteristics:
1. by adopting the fluorescence principle, a fluorescence detector is directly inserted into the oil sample in the form of a probe, and the content of the polar components in the oil sample is quantitatively detected.
2. The detection system is designed based on the fluorescence principle, comprises a detection induction end and a data control terminal such as a mobile phone or a tablet personal computer, and adopts an application program (app) installed on the data control terminal to connect the detection induction end and the data control terminal, so that the operation is simpler and more convenient.
The invention adopts the fluorescence optical principle, can avoid the complicated pretreatment such as sample purification and the like by selecting the specific excitation wavelength, does not need to use organic reagents and other auxiliary reagents, converts the fluorescence signal into a digital signal by utilizing the fluorescence property of substances contained in the edible oil and the characteristic that the edible oil with different polar component contents presents different fluorescence signals, visually presents the polar component contents of the edible oil by a built-in algorithm, and judges whether the contents exceed the standard or not.
It will be understood that the above embodiments are merely exemplary embodiments taken to illustrate the principles of the present invention, which is not limited thereto. It will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention, and these changes and modifications are also considered to be included in the scope of the invention.

Claims (4)

1. A method for quantitatively detecting the content of polar components in edible oil comprises the following steps:
exciting the edible oil with light of a specific wavelength;
collecting a fluorescent signal emitted by the edible oil under the excitation of the light with the specific wavelength;
obtaining a digital signal indicative of the intensity of the fluorescent signal; and
obtaining the content of the polar components of the edible oil corresponding to the digital signal for representing the intensity of the fluorescent signal based on the linear relation between the predetermined digital signal and the content of the polar components of the edible oil, wherein the linear relation between the predetermined digital signal and the content of the polar components of the edible oil is Y =0.062X-14.57, X is the value of the digital signal for representing the intensity of the fluorescent signal emitted by the edible oil under the excitation of the light with the specific wavelength, and Y is the value of the content of the polar components obtained by detecting the edible oil by using a national standard method; the specific wavelength is 450 nm; obtaining a digital signal indicative of the intensity of the fluorescent signal comprises:
converting the fluorescent signal into a corresponding alternating current signal;
converting the alternating current signals into corresponding direct current signals; and
converting the direct current telecommunications into corresponding digital signals, the corresponding digital signals being digital signals representing the intensity of the fluorescent signals;
the linear relation between the predetermined digital signal and the content of the polar components of the edible oil is obtained by firstly obtaining a plurality of groups of X values and Y values corresponding to the plurality of groups of X values and then carrying out linear fitting on the plurality of groups of X values and the plurality of groups of Y values.
2. An apparatus for quantitatively detecting the content of polar components in edible oil, comprising:
the excitation light source is used for emitting light with a specific wavelength to the edible oil;
a fluorescent signal receiving part for receiving a fluorescent signal emitted by the edible oil under the excitation of the light with the specific wavelength;
a signal processing section for processing the received fluorescence signal to obtain a digital signal representing an intensity of the fluorescence signal; and
a polar component content determining part, configured to obtain a polar component content of the edible oil corresponding to a digital signal representing an intensity of the fluorescent signal based on a linear relationship between a predetermined digital signal and the polar component content of the edible oil, where the linear relationship between the predetermined digital signal and the polar component content of the edible oil is Y =0.062X-14.57, where X is a value of the digital signal representing the intensity of the fluorescent signal emitted by the edible oil under excitation of the light with the specific wavelength, and Y is a polar component content value obtained by detecting the edible oil by a national standard method; the signal processing section includes:
a photoelectric conversion unit for converting the received fluorescent signal into a corresponding alternating current signal;
the alternating current-direct current conversion part is used for converting the alternating current signals into corresponding direct current signals; and
an analog-to-digital conversion part for converting the direct current signal into a corresponding digital signal, wherein the corresponding digital signal is a digital signal representing the intensity of the fluorescent signal; wherein the specific wavelength is 450 nm; or the linear relation between the predetermined digital signal and the content of the polar components of the edible oil is obtained by firstly obtaining a plurality of groups of X values and Y values corresponding to the plurality of groups of X values and then carrying out linear fitting on the plurality of groups of X values and the plurality of groups of Y values, or the equipment is handheld fluorescence detection equipment.
3. A system for quantitatively detecting the content of polar components in edible oil comprises:
the apparatus of claim 2, as a detection sensing terminal; and
the data control terminal is communicated with the detection induction end in a wireless communication mode;
the data control terminal receives a detection result of the content of the polar components of the edible oil from the detection induction terminal in a wireless communication mode, and displays the detection result of the content of the polar components of the edible oil, wherein the wireless communication mode is a Bluetooth communication mode, or wherein the detection induction terminal and the data control terminal are communicated in a wireless communication mode through an application program (app) installed on the data control terminal.
4. The system of claim 3, wherein the data control terminal comprises a cell phone or a tablet computer.
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CN106908426A (en) * 2017-02-04 2017-06-30 厦门斯坦道科学仪器股份有限公司 Specified plant oil safe screen is checked and surveys method, apparatus and system
CN107367468B (en) * 2017-08-03 2019-08-13 北京师范大学 Dissociate in a kind of edible oil fatty acid determination method
CN114460048B (en) * 2020-11-09 2024-03-22 中国科学院大连化学物理研究所 Method for measuring mass content of polar substances in edible oil by perovskite quantum dot fluorescence quenching method
CN115046936A (en) * 2022-03-25 2022-09-13 阿里云计算有限公司 Edible oil detection method and device

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Denomination of invention: Method, equipment, and system for quantitatively detecting the content of polar components in edible oil

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