CN102072893B - Fluorescent detection method of benzo (a) pyrene in edible oil - Google Patents
Fluorescent detection method of benzo (a) pyrene in edible oil Download PDFInfo
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Abstract
The invention discloses a fluorescent detection method of benzo (a) pyrene in edible oil, relates to a detection method of edible oil, and provides a fluorescent detection method of benzo (a) pyrene in edible oil, wherein the method is is simple, convenient and quick to operate, and the cost is low. The fluorescent detection method comprises the following steps: adding a dimethyl sulfoxide solvent in the edible oil; extracting and taking out the extracted product; s layering by standing or centrifuging; re-extracting the separated oil layer on the upper layer once, and collecting the dimethyl sulfoxide extraction liquid twice as liquid to be detected; plotting the liquid to be detected by a fluorescence spectrophotometer with derivative-constant energy isochronous scanning according to the following constant energy synchronizing fluorescence spectra conditions, and reading the fluorescence intensity of the benzo (a) pyrene by constant energy difference to identify the benzo (a) pyrene and measure roughly; and adding a second-order derivation function under the plotting conditions of the constant energy synchronizing fluorescence spectra to plot the derivative-constant energy synchronizing fluorescence spectra; reading the fluorescence intensity of the benzo (a) pyrene; and measuring the benzo (a) pyrene quantitatively by utilizing a continuous standard addition method.
Description
Technical field
The present invention relates to a kind of detection method of edible oil, especially relate to the fluorescence detection method of benzo in a kind of edible oil (a) pyrene.
Background technology
Palycyclic aromatic (PAHs) is one type of organic contaminant that extensively is present in environment and the varieties of food items, because it has strong lipophilicity, makes edible oil be more vulnerable to PAHs and pollutes.Grimmer and Hildebrandt disclose PAHs contents level in the vegetable oil to people first in nineteen sixty-eight.PAHs can be in relating to the oilseeds dry run because of incomplete combustion or pyrolysis combustion gas directly contact produce.Oilseeds also possibly pollute because of pollutants such as contact machine oil receive PAHs in processes such as mechanical harvest, transportation, processing.Once found in coconut oil that PAHs content was up to more than the 2000ppb.But, in some rural area, owing to lack dryer; In the harvest season, people often can see that the peasant is being covered with grain and oil raw material airings such as soybean on the bituminous pavement, in this case; In the product with these raw material production, must contain very high palycyclic aromatic material.
The edible oil matrix is complicated, and the concentration of PAHs is in the trace level, has potential multiple interfering material; Therefore before analyzing and testing, need do pre-service with enrichment component to be measured, to eliminate the interference of matrix to sample; To improve the sensitivity that detects, reduce detectability.So far, the PAHs preconditioning technique mainly contains the solid phase extraction and the novel supercritical CO of traditional liquid-liquid extraction method, high selectivity in the edible oil of reporting both at home and abroad
2Extractions etc., analyzing and testing combine coupling technique and gas chromatography and the flame ion or the mass spectral coupling technique of high performance liquid chromatography and fluorescence spectrum usually.These analytical approachs, not only pre-treatment process is loaded down with trivial details, time-consuming, and expensive, consumption solvent.Adopt chromatographic detection method to have the diluting effect of carrier inevitably, reduced sensitivity relatively, be not suitable for the popularization of method and the screening of testing agency of basic unit batch samples.
The constant-energy synchronous fluorescence spectroscopic methodology has characteristics such as high sensitivity, high selectivity, with the sensitivity that the coupling of derivative technique and constant-energy synchronous fluorescence technology can further improve the arrowband, makes spectrum simplify, reduce spectra overlapping, reduces the influence of scattered light; Make the bands of a spectrum characteristic more obvious, more help the discriminating and the detection of trace components in the potpourri.Measure in benzo (a) pyrene in the constant-energy synchronous fluorescence method, can utilize the selection of permanent energy difference, reduce even eliminate the interference of other fluorescent material, reduce requirement sample pre-treatments.
(Dimethyl sulfoxide DMSO) is a kind of organic compounds containing sulfur to dimethyl sulfoxide (DMSO), and molecular formula is (CH
3)
2SO, normal temperature down be the transparency liquid of no color or smell, and toxicity is extremely low, Heat stability is good, and most of organism such as ability is water-soluble, ethanol, propyl alcohol, benzene and chloroform are described as " alembroth ".But be insoluble to the aliphatic compound beyond the acetylene.As extraction solvent, it has following characteristics to dimethyl sulfoxide (DMSO) in the aromatic hydrocarbons extracting: (1) is high to the selectivity of aromatic hydrocarbon substance; (2) unrestrictedly miscible to aromatic hydrocarbon substance; (3) insoluble basically to lipoid material; (4) extraction temperature is low, and does not react with alkane, alkene, water; (5) no burn into is nontoxic; (6) available stripping process reclaims solvent; (7) selectivity strengthens benzo (a) pyrene fluorescence signal.
The applicant discloses benzo in a kind of tealeaves (a) pyrene in Chinese patent CN101876638A, benzo (k) is glimmering fear and fear in method for quick; Tealeaves is ground; Add the normal hexane ultrasonic extraction; Separate supernatant, add the dimethyl sulfoxide ultrasonic extraction, isolate the dimethyl sulfoxide extract and get testing sample solution; Use has the fluorospectrophotometer of derivative variable angle synchronous scanning function, according to benzo (a) pyrene, the non-linear variable angle synchronous fluorescent spectrum of the glimmering first order derivative of fearing and fearing of benzo (k) in the scanning pattern scanning testing sample solution; It is 84～87 peak value that benzo (a) pyrene reads scanning sequence, and benzo (k) is glimmering fears that to read scanning sequence be the peak value between 365～372, fears that to read scanning sequence be 930～1050 positive negative peak absolute value; Adopt continuous standard addition method or matrix coupling calibration curve, benzo (a) pyrene, the glimmering concentration of fearing and fearing of benzo (k) in the quantitative Analysis tealeaves solution to be measured.
Summary of the invention
The fluorescence detection method that the purpose of this invention is to provide benzo (a) pyrene in a kind of edible oil of simple and efficient to handle, cost.
The present invention includes following steps:
1) sample preparation: take by weighing edible oil in container, add dimethyl sulfoxide solvent, ultrasonic auxiliary extraction takes out standing demix or centrifugal layering, isolated upper strata oil reservoir re-extract 1 time, and the dimethyl sulfoxide (DMSO) extract of collecting 2 times is as liquid to be measured;
In step 1), the amount of said edible oil can be 0.5～2.0g, preferred 1.0g; The addition of said dimethyl sulfoxide (DMSO) can be 2～10mL, preferred 4mL; The output power of the said ultrasonic ultrasonoscope that adopts can be 250W; The time of said extraction can be 2～10min/ time, preferred 6min/ time; Said extraction times can be 2 times.
2) observational measurement: use the fluorospectrophotometer that has derivative-permanent energy synchronous scanning; Said liquid to be measured is surveyed and drawn by following constant-energy synchronous fluorescence spectrum condition, and permanent energy difference
reads the fluorescence intensity of benzo (a) pyrene it is differentiated and rough mensuration.
In step 2) in; The said method that reads the fluorescence intensity of benzo (a) pyrene can be: based on the constant-energy synchronous fluorescence spectroscopic methodology, by peak zero method read sample in zeroth order constant-energy synchronous fluorescence spectrum 390nm place fluorescence signal as the discriminating and rough mensuration of benzo (a) pyrene; Or read the derivative fluorescence intensity that sample is used as quantitative Analysis in the fluorescence intensity level at derivative-impossible energy synchronous fluorescent spectrum 401nm place; Said permanent energy difference
Preferred 1230cm
-1, in excitation wavelength, its wavelength scanning range should comprise this SPECTRAL REGION of 350～430nm, reads the fluorescence intensity of benzo (a) pyrene it is differentiated and rough mensuration.
3) quantitative measurment: in step 2) under the mapping condition of said constant-energy synchronous fluorescence spectrum; Add and put the second order derivation function; Carry out the mapping of derivative-constant-energy synchronous fluorescence spectrum, read the fluorescence intensity of benzo (a) pyrene, utilize continuous standard addition method the quantitative measurement of benzo (a) pyrene.
In step 3); The said method that reads the fluorescence intensity of benzo (a) pyrene can be: based on the constant-energy synchronous fluorescence spectroscopic methodology, by peak zero method read sample in zeroth order constant-energy synchronous fluorescence spectrum 390nm place fluorescence signal as the discriminating and rough mensuration of benzo (a) pyrene; Or read the derivative fluorescence intensity that sample is used as quantitative Analysis in the fluorescence intensity level at derivative-impossible energy synchronous fluorescent spectrum 401nm place.
The fluorospectrophotometer that has derivative-permanent energy synchronous scanning can adopt the multi-functional fluorospectrophotometer of MYF of PERKIN ELMER LS-50B fluorospectrophotometer, VARIAN ECLIPSE type fluorospectrophotometer and China like the U.S. etc.
The present invention is directed to the extraction and the existing deficiency of detection method of benzo (a) pyrene in the existing edible oil; Utilization is based on the derivative-advantage of constant-energy synchronous fluorescence technology and the characteristic of dimethyl sulfoxide (DMSO); Through selecting suitable solvent to combine to have the detection that detection method optionally realizes benzo (a) pyrene in the edible oil, purge processes such as reextraction, chromatography separation have been saved.Based on the derivative-advantage of constant-energy synchronous fluorescence technology and the characteristic of dimethyl sulfoxide (DMSO), set up benzo (a) pyrene in the ultrasonic assisted dimethyl sulfoxide extraction edible oil, detect the method for benzo (a) pyrene in conjunction with second derivative-constant-energy synchronous fluorescence method.Research shows that dimethyl sulfoxide (DMSO) is to analyze the fluorescence signal that solvent can selectivity enhanced sensitivity benzo (a) pyrene; The fluorescence signal that suppresses other palycyclic aromatic; Through selecting suitable energy difference; The constant-energy synchronous fluorescence technology can suppress the fluorescence signal of possibility interfering material, has greatly reduced the requirement to pre-treatment.The method for distilling of being set up has been simplified step greatly, need not saponification, concentrates, processes such as reextraction and chromatographic separation and purification, after sample only need pass through simple ultrasonic extraction, can pipette lower floor's dimethyl sulfoxide (DMSO) layer and be used for the constant-energy synchronous fluorescence method and detect.Can produce synergy in conjunction with dimethyl sulfoxide (DMSO) and derivative-constant-energy synchronous fluorescence technology, have the advantages such as accurate fast quantification that step is simple, analysis time short, selectivity is good, can realize trace benzo (a) pyrene in the edible oil.
Description of drawings
Fig. 1 is the zeroth order-constant-energy synchronous fluorescence spectrum of sunflower oil sample.In Fig. 1, horizontal ordinate is excitation wavelength (nm), and ordinate is relative intensity of fluorescence (a.u.); Permanent energy difference
(is seen arrow among Fig. 1) at the 390nm place and is the fluorescence peak of benzo (a) pyrene.
Fig. 2 is the second derivative-constant-energy synchronous fluorescence spectrum of sunflower oil sample.In Fig. 2, horizontal ordinate is identical with energy difference and Fig. 1, and ordinate is derivative fluorescence intensity (a.u.); Permanent energy difference
curve 1 is the spectrum of sample extraction liquid; Curve 2 to curve 5 is respectively to add benzo (a) pyrene standard solution 2ng/mL; 4ng/mL; 6ng/mL, the spectrum behind the 8ng/mL.Comparison by spectrogram can find out that benzo (a) pyrene peak value is bigger in second derivative-constant-energy synchronous fluorescence spectrum, and bands of a spectrum are narrower, not only can reduce the error of the value of reading, and can reduce the spectrum interference, improves sensitivity.
Fig. 3 is the standard addition method curve of sunflower oil sample.In Fig. 3, horizontal ordinate is mark-on concentration (ng/mL), and ordinate is derivative fluorescence intensity (a.u.).
Fig. 4 is the zeroth order-constant-energy synchronous fluorescence spectrum of blending stock sample.
Fig. 5 is the second derivative-constant-energy synchronous fluorescence spectrum of blending stock sample.
Fig. 6 is the standard addition method curve of blending stock sample.
Fig. 7 is the zeroth order-constant-energy synchronous fluorescence spectrum of coconut oil (BCR-458 standard reference materials) sample.
Fig. 8 is the second derivative-constant-energy synchronous fluorescence spectrum of coconut oil (BCR-458 standard reference materials) sample.
Fig. 9 is the standard addition method curve of coconut oil (BCR-458 standard reference materials) sample.
Embodiment
Following examples will combine accompanying drawing that the present invention is further described.
Embodiment 1: sunflower oil: the sunflower oil sample that takes by weighing 0.5g is in the serum bottle of 25mL; Add the 4mL dimethyl sulfoxide (DMSO); 59kHz, ultrasonic extraction 6min under the condition of 250W takes out standing demix; Isolated upper strata oil reservoir extracts once under similarity condition again, and the dimethyl sulfoxide (DMSO) extract of collecting twice is as liquid to be measured.Pipette the conventional quartzy fluorescent samples pond of 2mL solution to fluorophotometer, carry out the mapping of constant-energy synchronous fluorescence spectrum.Instrument parameter is provided with as follows: permanent energy difference
scans initial wavelength 260nm in excitation wavelength; Wavelength scanning range 220nm.Must be like zeroth order-constant-energy synchronous fluorescence spectrum of Fig. 1, benzo (a) the pyrene spectrum peak at the 390nm place among Fig. 1 can be used for the discriminating of benzo (a) pyrene and measures with rough.
Embodiment 2: similar with embodiment 1, its difference is to add puts the second order derivation function, obtains second derivative as shown in Figure 2-constant-energy synchronous fluorescence spectrum.Then adding 4 μ L concentration in the sample solution of 2mL is 1mg/L benzo (a) pyrene standard solution, carries out the mapping of second derivative-constant-energy synchronous fluorescence spectrum, mark-on 4 times.Adopt peak zero method to read the signal strength values of sample in the 401nm place; As the derivative fluorescence intensity that quantitative Analysis is used, mapping adds curve like the standard of Fig. 3, and the linear fit equation that standard adds curve is Y=405.64+173.03X; Related coefficient is 0.9999, and is linear good.Getting sunflower oil sample extraction liquid benzo (a) pyrene content thus is 2.34 μ g/L, and the content that is scaled benzo in the sunflower oil (a) pyrene then is 37.4 μ g/kg.Can be found out that by Fig. 2 benzo (a) pyrene relative peak is bigger in second derivative-constant-energy synchronous fluorescence spectrum, bands of a spectrum are narrow, can reduce the error of the value of reading, and can reduce spectrum and disturb, and strengthen the resolution characteristic of characteristic spectrum fine structure, improve sensitivity.
Embodiment 3: blending stock: take by weighing 0.5g blending stock sample in the serum bottle of 25mL; Add the 4mL dimethyl sulfoxide solvent; 59kHz, the ultrasound condition of 250W be extraction 6min down, takes out standing demix; Isolated upper strata oil reservoir extracts once under similarity condition again, and the dimethyl sulfoxide (DMSO) extract of collecting twice is as liquid to be measured.Pipette the conventional quartzy fluorescent samples pond of 2mL solution to fluorophotometer, carry out the mapping of constant-energy synchronous fluorescence spectrum.Instrument parameters are set as follows: Constant energy difference
scanning start excitation wavelength 260nm; wavelength scan range of 220nm.Obtain zeroth order as shown in Figure 4-constant-energy synchronous fluorescence spectrum.Add and put the second order derivation function, obtain second derivative as shown in Figure 5-constant-energy synchronous fluorescence spectrum.Adopt continuous standard addition method quantitative, data read mode and adopt peak zero method, and mapping adds curve like the standard of Fig. 6.The linear fit equation that standard adds curve is Y=170.2+302.3X, and related coefficient is 0.9999, and is linear good.Get benzo (a) pyrene that the blending stock extract contains 0.563 μ g/L thus, the content that is scaled benzo in the blending stock (a) pyrene then is 9.01 μ g/kg.
Embodiment 4: coconut oil: the coconut oil sample that takes by weighing 1.0g is in the 25mL serum bottle; Add the 4mL dimethyl sulfoxide (DMSO); At 59kHz, the ultrasound condition of 250W is extraction 6min down, takes out standing demix; Isolated upper strata oil reservoir extracts once under similarity condition again, and the dimethyl sulfoxide (DMSO) extract of collecting twice is as liquid to be measured.Pipette the conventional quartzy fluorescent samples pond of 2mL solution to fluorophotometer, carry out the mapping of constant-energy synchronous fluorescence spectrum.Instrument parameters are set as follows: Constant energy difference
scanning start excitation wavelength 260nm; wavelength scan range of 220nm.Obtain constant-energy synchronous fluorescence spectrum as shown in Figure 7.Add and put the second order derivation function, obtain second derivative as shown in Figure 8-constant-energy synchronous fluorescence spectrum.Adopt continuous standard addition method quantitative, data read mode and adopt peak zero method, and mapping adds curve like the standard of Fig. 9.The linear fit equation that standard adds curve is Y=29.8+288.3X, and related coefficient is 0.9994, and is linear good.Get benzo (a) pyrene that coconut oil sample extraction liquid contains 0.102 μ g/L thus, the content that is scaled benzo in the coconut oil (a) pyrene then is 0.816 μ g/kg.
Embodiment 5: coconut oil: (BCR 458 standard reference materialss; Benzo (a) pyrene content reference value is 0.93 μ g/kg) take by weighing 2.0g coconut oil sample in the serum bottle of 25mL, add the 4mL dimethyl sulfoxide (DMSO), 59kHz; The ultrasound condition of 250W is extraction 6min down; Take out standing demix, isolated upper strata oil reservoir extracts under similarity condition 1 time again, and the dimethyl sulfoxide (DMSO) extract of collecting 2 times is as liquid to be measured.Pipette the conventional quartzy fluorescent samples pond of 2mL solution to fluorophotometer, carry out the mapping of constant-energy synchronous fluorescence spectrum.Instrument parameter is provided with as follows: permanent energy difference
adds puts the second order derivation function.Mapping derivative-constant-energy synchronous fluorescence spectrum is measured by standard addition method.Recording benzo in the coconut oil (a) pyrene content is 0.96 μ g/kg.
Embodiment 6: peanut oil: take by weighing 0.5g peanut oil in the serum bottle of 25mL; Add the 2mL dimethyl sulfoxide (DMSO); 59kHz, the ultrasound condition of 250W be extraction 10min down, takes out standing demix; Isolated upper strata oil reservoir extracts once under similarity condition again, and the dimethyl sulfoxide (DMSO) extract of collecting twice is as liquid to be measured.Pipette the conventional quartzy fluorescent samples pond of 2mL solution to fluorophotometer, carry out the mapping of constant-energy synchronous fluorescence spectrum.Instrument parameter is provided with as follows: permanent energy difference
adds puts the second order derivation function.Mapping derivative-constant-energy synchronous fluorescence spectrum is measured by standard addition method, measure in the peanut oil sample benzo (a) pyrene content be 821 μ g/kg.
Embodiment 7: sunflower oil: take by weighing the 0.5g sunflower oil in the serum bottle of 25mL; Add the 10mL dimethyl sulfoxide (DMSO); 59kHz, the ultrasound condition of 250W be extraction 2min down, takes out standing demix; Isolated upper strata oil reservoir extracts once under similarity condition again, and the dimethyl sulfoxide (DMSO) extract of collecting twice is as liquid to be measured.Pipette the conventional quartzy fluorescent samples pond of 2mL solution to fluorophotometer, carry out the mapping of constant-energy synchronous fluorescence spectrum.Instrument parameter is provided with as follows: permanent energy difference
adds puts the second order derivation function.Mapping derivative-constant-energy synchronous fluorescence spectrum, and measure by standard addition method, measure in the sunflower oil sample benzo (a) pyrene content be 36.5 μ g/kg.
Embodiment 8: peanut oil: take by weighing 1.0g peanut oil in the serum bottle of 25mL; Add the 5mL dimethyl sulfoxide (DMSO); At 59kHz, the ultrasound condition of 250W is extraction 8min down, takes out standing demix; Isolated upper strata oil reservoir extracts once under similarity condition again, and the dimethyl sulfoxide (DMSO) extract of collecting twice is as liquid to be measured.Pipette the conventional quartzy fluorescent samples pond of 2mL solution to fluorophotometer, carry out the mapping of constant-energy synchronous fluorescence spectrum.Instrument parameter is provided with as follows: permanent energy difference
adds puts the second order derivation function.Mapping derivative-constant-energy synchronous fluorescence spectrum, and by the standard addition method measurement.Measure in the peanut oil benzo (a) pyrene content be 1.99 μ g/kg.
Claims (9)
1. the fluorescence detection method of benzo (a) pyrene in the edible oil is characterized in that may further comprise the steps:
1) sample preparation: take by weighing edible oil in container, add dimethyl sulfoxide solvent, ultrasonic auxiliary extraction takes out standing demix or centrifugal layering, isolated upper strata oil reservoir re-extract 1 time, and the dimethyl sulfoxide (DMSO) extract of collecting 2 times is as liquid to be measured;
2) observational measurement: use the fluorospectrophotometer that has derivative-permanent energy synchronous scanning; Said liquid to be measured is surveyed and drawn by following constant-energy synchronous fluorescence spectrum condition, and permanent energy difference
reads the fluorescence intensity of benzo (a) pyrene it is differentiated and rough mensuration;
3) quantitative measurment: in step 2) under the mapping condition of said constant-energy synchronous fluorescence spectrum; Add and put the second order derivation function; Carry out the mapping of derivative-constant-energy synchronous fluorescence spectrum, read the fluorescence intensity of benzo (a) pyrene, utilize continuous standard addition method the quantitative measurement of benzo (a) pyrene.
2. the fluorescence detection method of benzo (a) pyrene is characterized in that in step 1) in the edible oil as claimed in claim 1, and the amount of said edible oil is 0.5～2.0g; The addition of said dimethyl sulfoxide (DMSO) is 2～10mL.
3. the fluorescence detection method of benzo (a) pyrene in the edible oil as claimed in claim 2, the amount that it is characterized in that said edible oil is 1.0g; The addition of said dimethyl sulfoxide (DMSO) is 4mL.
4. the fluorescence detection method of benzo (a) pyrene is characterized in that in step 1) the output power of the said ultrasonic ultrasonoscope that adopts is 250W in the edible oil as claimed in claim 1.
5. the fluorescence detection method of benzo (a) pyrene is characterized in that in step 1) in the edible oil as claimed in claim 1, and the time of said extraction is 2～10min/ time.
6. the fluorescence detection method of benzo (a) pyrene in the edible oil as claimed in claim 5, the time that it is characterized in that said extraction is 6min/ time.
7. the fluorescence detection method of benzo (a) pyrene in the edible oil as claimed in claim 1; It is characterized in that in step 2) in; The said method that reads the fluorescence intensity of benzo (a) pyrene is: based on the constant-energy synchronous fluorescence spectroscopic methodology, by peak zero method read sample in zeroth order constant-energy synchronous fluorescence spectrum 390nm place fluorescence signal as the discriminating and rough mensuration of benzo (a) pyrene; Or
Read the derivative fluorescence intensity that sample is used as quantitative Analysis in the fluorescence intensity level at derivative-constant-energy synchronous fluorescence spectrum 401nm place.
8. the fluorescence detection method of benzo (a) pyrene is characterized in that in step 2 in the edible oil as claimed in claim 1) in, said permanent energy difference
Be 1230cm
-1
9. the fluorescence detection method of benzo (a) pyrene in the edible oil as claimed in claim 1; It is characterized in that in step 3); The said method that reads the fluorescence intensity of benzo (a) pyrene is: based on the constant-energy synchronous fluorescence spectroscopic methodology, by peak zero method read sample in zeroth order constant-energy synchronous fluorescence spectrum 390nm place fluorescence signal as the discriminating and rough mensuration of benzo (a) pyrene; Or
Read the derivative fluorescence intensity that sample is used as quantitative Analysis in the fluorescence intensity level at derivative-constant-energy synchronous fluorescence spectrum 401nm place.
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| CN102914523B (en) * | 2011-08-04 | 2015-12-02 | 湖北理工学院 | A kind of synchronous fluorimetry measuring pyrene in the fish gill |
| CN106370635B (en) * | 2016-08-31 | 2021-04-06 | 厦门斯坦道科学仪器股份有限公司 | Method, equipment and system for quantitatively detecting content of polar components in edible oil |
| CN109580318A (en) * | 2018-12-11 | 2019-04-05 | 江南大学 | A kind of method of distributing Solid Phase Extraction processing edible oil sample detection benzo [a] pyrene |
| CN110045047A (en) * | 2019-05-23 | 2019-07-23 | 开化县检验检测研究院 | A kind of method of liquid-liquid micro-extraction processing edible vegetable oil sample detection benzo [a] pyrene |
| CN112557362B (en) * | 2020-12-04 | 2022-08-23 | 厦门大学 | Synchronous fluorescence spectrum detection method using LED light source as continuous wave excitation light source |
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| JP2002168788A (en) * | 2000-11-29 | 2002-06-14 | Takashi Inaga | DETECTION METHOD AND DEVICE OF BENZO[a]PYRENE |
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| JP2002168788A (en) * | 2000-11-29 | 2002-06-14 | Takashi Inaga | DETECTION METHOD AND DEVICE OF BENZO[a]PYRENE |
| RU2236916C1 (en) * | 2003-06-30 | 2004-09-27 | Индивидуальный Предприниматель Без Образования Юридического Лица Ващенко Юрий Ефимович | Method of determining weight concentration of benzo-a-pyrene in soil |
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