CN106367354A - Application of potato whole powder in preparing PDA (potato dextrose agar) medium - Google Patents
Application of potato whole powder in preparing PDA (potato dextrose agar) medium Download PDFInfo
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- CN106367354A CN106367354A CN201610740833.0A CN201610740833A CN106367354A CN 106367354 A CN106367354 A CN 106367354A CN 201610740833 A CN201610740833 A CN 201610740833A CN 106367354 A CN106367354 A CN 106367354A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/76—Undefined extracts from plants
Abstract
The invention discloses an application of potato whole powder in preparing a PDA (potato dextrose agar) medium, and belongs to the field of microbial culture mediums. The invention also discloses a potato whole powder medium, wherein the potato whole powder medium consists of the following components according to proportions: 10-20g of the potato whole powder, 2-10g of glucose, 12-20g of agar powder and 1000ml of water. The invention also discloses an application of the potato whole powder medium in fungal culture. According to the potato whole powder medium provided by the invention, such nutrient substances as starch, protein, vitamins, mineral substances and the like in potatoes are completely reserved, so that comprehensive nutrition is guaranteed, and edible fungi, on the potato whole powder medium, are high in mycelia growth speed and vigorous in growth; moreover, the potato whole powder medium provided by the invention is simple in preparation method and the preparation method is free from such processes as preparing potato juice and the like, so that a preparation time is greatly shortened; in addition, the potato whole powder medium provided by the invention is low in production cost; and the potato whole powder medium provided by the invention, since all ingredients are in the form of dry powder, are quite convenient to store and transport.
Description
Technical field
The invention belongs to microbiological culture media field is and in particular to application on preparing pda culture medium for the potato full-powder；
Further relate to a kind of potato full-powder culture medium, and the preparation method of this culture medium and purposes.
Background technology
Pda culture medium (potato dextrose agar (abbreviation of potato dextrose agar (medium))
It is a kind of conventional culture medium, be usually used in the funguses such as culture yeasts bacterium, mycete, edible fungi.Pda culture medium versatility is good, can be full
The basic growth of foot overwhelming majority edible fungi requires, but because of first potato juice to be produced in its preparation process, that is, takes fresh nothing
Go mouldy the Rhizoma Solani tuber osi no sprouting, and cleans peeling, is cut into small pieces, adds water well-done, and filtered through gauze produces potato juice；This extracting juice mistake
Journey not only makes that the preparation process of pda culture medium bothers very much, time-consuming, and a lot of battalion of Rhizoma Solani tuber osi during filtering extracting juice
Form and point all throw away, both caused significant wastage, also improved the production cost of pda culture medium.In fact, in Rhizoma Solani tuber osi
In dry, 76% can serve as the starch of excellent carbon source, and 7.2% can serve as the protein of excellent nitrogen source, but due to it
In water, dissolubility is low, it is opaque to easily cause culture medium, thus by filtering, their overwhelming majority is all thrown away.
Prepare cumbersome problem for pda culture medium, market occurs in that and directly pda medium component is condensed into
Solid, adds distilled water or deionized water during use, then heating for dissolving, and subpackage sterilizes, and so that the preparation of pda culture medium is become
Simply.These are condensed in the pda culture medium of solid and instead of traditional Rhizoma Solani tuber osi with potato starch or Rhizoma Solani tuber osi leaching powder
First medicine is opened in juice, potato dextrose agar (pda) culture medium that star Bioisystech Co., Ltd as extensive and profound in meaning in Beijing produces and Ningxia
(number of patent application is the mould medium of industry company limited: is all 201310594078.6) to leach powder using Rhizoma Solani tuber osi to replace horse
Bell potato juice, Rhizoma Solani tuber osi leaches the enzymatic hydrolysate that powder is Rhizoma Solani tuber osi, and predominantly microorganism culturing provides carbon source and nitrogen source.Rhizoma Solani tuber osi is soaked
Going out powder is with Rhizoma Solani tuber osi as raw material, and size-reduced, defibrination, dipping, extracting, saccharifying enzymolysis, proteolysiss, fine straining, cold nebulization are done
Dry form, ultra-fine, instant, light brown to auburn powder, there is hygroscopicity.And the difco of importtmPda culture medium is then
Instead of potato juice with potato starch.But, these pda culture medium still eliminate most of starch and the egg of Rhizoma Solani tuber osi
The nutrient substance such as white matter, nutritional labeling is not comprehensive.In addition, this kind of pda culture medium leaches powder due to potato starch or Rhizoma Solani tuber osi
Preparation cost high, the production cost causing produced pda culture medium solid portion is high, prices are rather stiff it is difficult to obtain
Extensively apply.
Potato full-powder is with fresh potato as raw material, cleaned, peeling, and section is precooked, cooling, steaming and decocting, smash to mud etc.
Technical process, then through piece bits shape or fine powdered product obtained from dehydrate, it is referred to as potato full-powder.Compared to Rhizoma Solani tuber osi
Leach powder and potato starch, in potato full-powder by contain in Rhizoma Solani tuber osi 76% starch, 7.2% protein, 0.5%
Fat, the nutrient substance such as vitamin and mineral substantially retain comprehensively, not only comprehensive nutrition and nutrient substance enrich, and do not have
Nutritious composition wastes, and the cost of production is relatively low.Potato full-powder be mainly used at present quick frozen food, puffed food, bread,
Bait, fast food and baby food etc..
Through retrieval, do not find the report that potato full-powder is applied on preparing pda culture medium.
Content of the invention
For existing pda culture medium preparation trouble, waste the shortcomings of potato nutritional material, high cost, price, this
Goal of the invention is to provide application on preparing pda culture medium for the potato full-powder.
Another object of the present invention is to provide a kind of potato full-powder culture medium.
The present invention the 3rd purpose is to provide the preparation method of above-mentioned potato full-powder culture medium.
The present invention the 4th purpose is to provide purposes on culture funguses for the above-mentioned potato full-powder culture medium.
The purpose of the present invention to be realized by following technical solution:
The invention provides application on preparing pda culture medium for the potato full-powder.
Described pda is the abbreviation of potato dextrose agar (potato dextrose agar).
Described potato full-powder refers to fresh potato as raw material, cleaned, peeling, and section is precooked, cooling, steams
Boil, the technical process such as smash to mud, then through piece bits shape or fine powdered product obtained from dehydrate, be referred to as potato full-powder.
Above-mentioned potato full-powder is easy to commercially obtain, and such as buys from United Food Co., Ltd. Fu Xinghua
Deng.
Present invention also offers a kind of potato full-powder culture medium, in described potato full-powder culture medium, contain Rhizoma Solani tuber osi
Quan Fen.
Above-mentioned potato full-powder culture medium, its constituent and its ratio are:
The weight of the potato full-powder described in above-mentioned potato full-powder culture medium is than preferably 13～18g.Excellent further
Elect 16g as.
The weight of the glucose described in above-mentioned potato full-powder culture medium is than preferably 5～8g.More preferably 6g.
The weight of the agar powder described in above-mentioned potato full-powder culture medium is than preferably 14～16g.More preferably
15g.
The preparation method of above-mentioned potato full-powder culture medium: weigh potato full-powder, glucose and agar according to weight ratio
Powder, mix homogeneously, it is subsequently adding in container (as triangular flask), is proportionally added into water, shakes up, seal up sealed membrane or kraft paper, 121
DEG C sterilizing 30min, standby.
The using method of above-mentioned potato full-powder culture medium: the potato full-powder culture medium of bacterium of just having been gone out, before solidification
(more than 40 DEG C), while hot aseptically, shaken well, pour in aseptic culture dish, for inoculation.The horse having solidified
Bell potato full powder culture medium, after heating fusing in microwave oven, with above step operation.
Potato full-powder, glucose and agar powder can also first be mixed by above-mentioned potato full-powder culture medium according to aforementioned proportion
Close uniformly, be condensed into solid, powdery thing.The solid, powdery thing of described potato full-powder culture medium can carry out factorial praluction, makes
The application of potato full-powder culture medium is easier.
The using method of the solid, powdery thing of above-mentioned potato full-powder culture medium, including proportionally by above-mentioned solid, powdery
Thing is added to the water, and shakes up, and seals up sealed membrane or kraft paper, 121 DEG C of sterilizing 30min.
Application on culture funguses for the above-mentioned potato full-powder culture medium.
Funguses described in above-mentioned application refer to yeast, mycete or edible fungi etc..
Edible fungi described in above-mentioned application refers to Lentinus Edodess, Pleurotus ostreatus, Auricularia, Auricularia polytricha (Mout) Sacc., Flammulina velutiper (Fr.) Sing, Agaricus bisporuss, white
Clever mushroom, Pleurotus eryngii, Pleurotus ferulae Lanzi or Pholiota nameko etc..
Compared with existing pda culture medium, the present invention has the advantage that and beneficial effect: the difco of (1) and importtm
Pda culture medium is compared with traditional pda culture medium, edible fungi mycelial growth rate in potato full-powder culture medium of the present invention
Hurry up, and grow vigorous；(2) potato full-powder used by potato full-powder culture medium of the present invention is by the starch in Rhizoma Solani tuber osi, albumen
The nutrition such as matter, vitamin and mineral all preserve, and not do not run off, comprehensive nutrition and abundant, can preferably meet edible fungi
Growth requires；(3) potato full-powder culture medium preparation of the present invention is simple, directly weighs required composition, mix homogeneously, sterilizing is
Can, eliminate Rhizoma Solani tuber osi peel, steaming and decocting and filter the process such as extracting juice, preparation time greatly shortens；(4) potato full-powder of the present invention
Culture medium replaces traditional potato juice with potato full-powder, and its production cost substantially reduces；And greatly reduce Rhizoma Solani tuber osi
The waste of raw material；(5) potato full-powder used by potato full-powder culture medium of the present invention, due to being dry powder, is difficult to go mouldy or goes out
Bud, is easy to preserve, the room temperature lower shelf-life is up to 15 years；And due to of many uses, manufacturer is numerous, wide material sources it is easy to
It is commercially available from market.
Brief description:
Fig. 1. Pleurotus ostreatus ccef89 is in difcotmMycelial growth photo in pda culture medium.
Fig. 2. Pleurotus ostreatus ccef89 mycelial growth photo in potato full-powder culture medium a.
Fig. 3. Pleurotus ostreatus ccef89 mycelial growth photo in potato full-powder culture medium b.
Fig. 4. Pleurotus ostreatus ccef89 mycelial growth photo in potato full-powder culture medium c.
Fig. 5. Lentinus Edodess 808 are in difcotmMycelial growth photo in pda culture medium.
Fig. 6. Lentinus Edodess 808 mycelial growth photo in potato full-powder culture medium a.
Fig. 7. Lentinus Edodess 808 mycelial growth photo in potato full-powder culture medium b.
Fig. 8. Lentinus Edodess 808 mycelial growth photo in potato full-powder culture medium c.
Fig. 9. Auricularia black 29 is in difcotmMycelial growth photo in pda culture medium.
Figure 10. Auricularia black 29 mycelial growth photo in potato full-powder culture medium a.
Figure 11. Auricularia black 29 mycelial growth photo in potato full-powder culture medium b.
Figure 12. Auricularia black 29 mycelial growth photo in potato full-powder culture medium c.
Figure 13. Auricularia polytricha (Mout) Sacc. Soviet Union hair 3 is in difcotmMycelial growth photo in pda culture medium.
Figure 14. Auricularia polytricha (Mout) Sacc. Soviet Union hair 3 mycelial growth photo in potato full-powder culture medium a.
Figure 15. Auricularia polytricha (Mout) Sacc. Soviet Union hair 3 mycelial growth photo in potato full-powder culture medium b.
Figure 16. Auricularia polytricha (Mout) Sacc. Soviet Union hair 3 mycelial growth photo in potato full-powder culture medium c.
Figure 17. Agaricus bisporuss w192 is in difcotmMycelial growth photo in pda culture medium.
Figure 18. Agaricus bisporuss w192 mycelial growth photo in potato full-powder culture medium a.
Figure 19. Agaricus bisporuss w192 mycelial growth photo in potato full-powder culture medium b.
Figure 20. Agaricus bisporuss w192 mycelial growth photo in potato full-powder culture medium c.
Figure 21. Flammulina velutiper (Fr.) Sing aura 19 is in difcotmMycelial growth photo in pda culture medium.
Figure 22. Flammulina velutiper (Fr.) Sing aura 19 mycelial growth photo in potato full-powder culture medium a.
Figure 23. Flammulina velutiper (Fr.) Sing aura 19 mycelial growth photo in potato full-powder culture medium b.
Figure 24. Flammulina velutiper (Fr.) Sing aura 19 mycelial growth photo in potato full-powder culture medium c.
Figure 25. Pleurotus eryngii 3898 is in difcotmMycelial growth photo in pda culture medium.
Figure 26. Pleurotus eryngii 3898 mycelial growth photo in potato full-powder culture medium a.
Figure 27. Pleurotus eryngii 3898 mycelial growth photo in potato full-powder culture medium b.
Figure 28. Pleurotus eryngii 3898 mycelial growth photo in potato full-powder culture medium c.
Figure 29. Pleurotus nebrodensis middle peasant No. 1 is in difcotmMycelial growth photo in pda culture medium.
Figure 30. Pleurotus nebrodensis middle peasant No. 1 mycelial growth photo in potato full-powder culture medium a.
Figure 31. Pleurotus nebrodensis middle peasant No. 1 mycelial growth photo in potato full-powder culture medium b.
Figure 32. Pleurotus nebrodensis middle peasant No. 1 mycelial growth photo in potato full-powder culture medium c.
Figure 33. Pholiota nameko early life 2 is in difcotmMycelial growth photo in pda culture medium.
Figure 34. Pholiota nameko early raw No. 2 mycelial growth photos in potato full-powder culture medium a.
Figure 35. Pholiota nameko early raw No. 2 mycelial growth photos in potato full-powder culture medium b.
Figure 36. Pholiota nameko early raw No. 2 mycelial growth photos in potato full-powder culture medium c.
Figure 37. Pleurotus ostreatus ccef89 is in difcotmMycelial growth photo in pda culture medium.
Figure 38. Pleurotus ostreatus ccef89 mycelial growth photo in potato full-powder culture medium d.
Figure 39. Pleurotus ostreatus ccef89 mycelial growth photo in potato full-powder culture medium e.
Figure 40. Pleurotus ostreatus ccef89 mycelial growth photo in potato full-powder culture medium f.
Figure 41. Lentinus Edodess 808 are in difcotmMycelial growth photo in pda culture medium.
Figure 42. Lentinus Edodess 808 mycelial growth photo in potato full-powder culture medium d.
Figure 43. Lentinus Edodess 808 mycelial growth photo in potato full-powder culture medium e.
Figure 44. Lentinus Edodess 808 mycelial growth photo in potato full-powder culture medium f.
Figure 45. Auricularia black 29 is in difcotmMycelial growth photo in pda culture medium.
Figure 46. Auricularia black 29 mycelial growth photo in potato full-powder culture medium d.
Figure 47. Auricularia black 29 mycelial growth photo in potato full-powder culture medium e.
Figure 48. Auricularia black 29 mycelial growth photo in potato full-powder culture medium f.
Figure 49. Auricularia polytricha (Mout) Sacc. Soviet Union hair 3 is in difcotmMycelial growth photo in pda culture medium.
Figure 50. Auricularia polytricha (Mout) Sacc. Soviet Union hair 3 mycelial growth photo in potato full-powder culture medium d.
Figure 51. Auricularia polytricha (Mout) Sacc. Soviet Union hair 3 mycelial growth photo in potato full-powder culture medium e.
Figure 52. Auricularia polytricha (Mout) Sacc. Soviet Union hair 3 mycelial growth photo in potato full-powder culture medium f.
Figure 53. Agaricus bisporuss w192 is in difcotmMycelial growth photo in pda culture medium.
Figure 54. Agaricus bisporuss w192 mycelial growth photo in potato full-powder culture medium d.
Figure 55. Agaricus bisporuss w192 mycelial growth photo in potato full-powder culture medium e.
Figure 56. Agaricus bisporuss w192 mycelial growth photo in potato full-powder culture medium f.
Figure 57. Flammulina velutiper (Fr.) Sing aura 19 is in difcotmMycelial growth photo in pda culture medium.
Figure 58. Flammulina velutiper (Fr.) Sing aura 19 mycelial growth photo in potato full-powder culture medium d.
Figure 59. Flammulina velutiper (Fr.) Sing aura 19 mycelial growth photo in potato full-powder culture medium e.
Figure 60. Flammulina velutiper (Fr.) Sing aura 19 mycelial growth photo in potato full-powder culture medium f.
Figure 61. Pleurotus eryngii 3898 is in difcotmMycelial growth photo in pda culture medium.
Figure 62. Pleurotus eryngii 3898 mycelial growth photo in potato full-powder culture medium d.
Figure 63. Pleurotus eryngii 3898 mycelial growth photo in potato full-powder culture medium e.
Figure 64. Pleurotus eryngii 3898 mycelial growth photo in potato full-powder culture medium f.
Figure 65. Pleurotus nebrodensis middle peasant No. 1 is in difcotmMycelial growth photo in pda culture medium.
Figure 66. Pleurotus nebrodensis middle peasant No. 1 mycelial growth photo in potato full-powder culture medium d.
Figure 67. Pleurotus nebrodensis middle peasant No. 1 mycelial growth photo in potato full-powder culture medium e.
Figure 68. Pleurotus nebrodensis middle peasant No. 1 mycelial growth photo in potato full-powder culture medium f.
Figure 69. Pholiota nameko early life 2 is in difcotmMycelial growth photo in pda culture medium.
Figure 70. Pholiota nameko early raw No. 2 mycelial growth photos in potato full-powder culture medium d.
Figure 71. Pholiota nameko early raw No. 2 mycelial growth photos in potato full-powder culture medium e.
Figure 72. Pholiota nameko early raw No. 2 mycelial growth photos in potato full-powder culture medium f.
Specific embodiment:
Below by way of example, the invention will be further described, but the present invention is not limited in any way.
The preparation of the potato full-powder culture medium of the different glucose content of embodiment 1
It is prepared as follows:
The composition of potato full-powder culture medium of the different glucose content of table 1 and its ratio
According to weighing potato full-powder (United Food Co., Ltd. Fu Xinghua's production), glucose and fine jade shown in table 1 respectively
Cosmetics, mix homogeneously, be then added in 3 different 1000ml triangular flasks, add 800ml water, shake up, seal up sealed membrane or
Kraft paper, 121 DEG C of sterilizing 30min.When not solidifying, pour culture dish into, culture medium 20- poured into by the culture dish of each diameter 9cm
25ml, solidification is stand-by.
The preparation of the potato full-powder culture medium of the different potato full-powder content of embodiment 2
It is prepared as follows:
According to weighing potato full-powder (United Food Co., Ltd. Fu Xinghua's production), glucose and fine jade shown in table 2 respectively
Cosmetics, mix homogeneously, be then added in 3 different 1000ml triangular flasks, add 800ml water, shake up, seal up sealed membrane or
Kraft paper, 121 DEG C of sterilizing 30min.When not solidifying, culture dish, culture medium 20- poured into by the culture dish of each diameter 9cm
25ml, solidification is stand-by.
The composition of potato full-powder culture medium of the different potato full-powder content of table 2 and its ratio
Embodiment 3 is in difcotmEdible fungi contrast test is cultivated on pda culture medium and potato full-powder culture medium
(1) test material:
1st, culture medium:
(1) the potato full-powder culture medium (numbering is a, b and c) prepared in embodiment 1.
(2)difcotmPda culture medium, is made by operating instruction.
(3) common pda culture medium, conventionally prepares.
2nd, edible mushroom strainses: Pleurotus ostreatus ccef89, Lentinus Edodess 808, Auricularia are black 29, Auricularia polytricha (Mout) Sacc. Soviet Union hair 3, Agaricus bisporuss
W192, Flammulina velutiper (Fr.) Sing are aura 19, Pleurotus eryngii 3898, Pleurotus nebrodensis middle peasant No. 1, Pholiota nameko early 9 kinds of edible mushroom strainses such as raw No. 2.Strain is equal
From INST OF AGRICULTURAL RESOURCES.
(2) test method:
Will be aura to black to Pleurotus ostreatus ccef89, Lentinus Edodess 808, Auricularia 29, Auricularia polytricha (Mout) Sacc. Soviet Union hair 3, Agaricus bisporuss w192, Flammulina velutiper (Fr.) Sing
19th, early 9 kinds of edible mushroom strainses such as raw No. 2 are lived in common pda culture medium for Pleurotus eryngii 3898, Pleurotus nebrodensis middle peasant No. 1, Pholiota nameko
Change, then use the card punch of diameter 5mm, in colony edge punching, make that cell age is identical, size identical truffle is seeded in this respectively
Invention potato full-powder culture medium (numbering a, b, c) and difcotmThe central authorities of pda culture medium, are sealed up with sealed membrane, each bacterial strain
3 repetitions.Again they are placed in same incubator and cultivate 7 days under the conditions of 24 DEG C, lucifuge.Observe colony diameter, and take pictures.
Result Pleurotus ostreatus (see Fig. 1～Fig. 4), Lentinus Edodess (see Fig. 5～Fig. 8), Auricularia (see Fig. 9～Figure 12), Auricularia polytricha (Mout) Sacc. are (see figure
13～Figure 16), Agaricus bisporuss (see Figure 17～Figure 20), Flammulina velutiper (Fr.) Sing (see Figure 21～Figure 24), Pleurotus eryngii (see Figure 25～Figure 28), white
9 kinds of edible fungi such as clever mushroom (see Figure 29～Figure 32), Pholiota nameko (see Figure 33～Figure 36) are in potato full-powder culture medium of the present invention
The speed of growth is substantially than in difcotmFast growth in pda culture medium.Illustrate that edible fungi is not only complete in Rhizoma Solani tuber osi of the present invention
In powder culture medium can normal growth, and ratio is in difcotmFast growth in pda culture medium.
Embodiment 4 is in difcotmEdible fungi contrast test is cultivated on pda culture medium and potato full-powder culture medium of the present invention
(1) test material:
1st, culture medium:
(1) the potato full-powder culture medium (numbering is d, e and f) prepared in embodiment 2.
(2)difcotmPda culture medium, is made by operating instruction.
(3) common pda culture medium, conventionally prepares.
2nd, edible mushroom strainses: Pleurotus ostreatus ccef89, Lentinus Edodess 808, Auricularia are black 29, Auricularia polytricha (Mout) Sacc. Soviet Union hair 3, Agaricus bisporuss
W192, Flammulina velutiper (Fr.) Sing are aura 19, Pleurotus eryngii 3898, Pleurotus nebrodensis middle peasant No. 1, Pholiota nameko early 9 kinds of edible mushroom strainses such as raw No. 2.Strain is equal
From INST OF AGRICULTURAL RESOURCES.
(2) test method: by black to Pleurotus ostreatus ccef89, Lentinus Edodess 808, Auricularia 29, Auricularia polytricha (Mout) Sacc. Soviet Union hair 3, Agaricus bisporuss
W192, Flammulina velutiper (Fr.) Sing are aura 19, Pleurotus eryngii 3898, Pleurotus nebrodensis middle peasant No. 1, Pholiota nameko early 9 kinds of edible mushroom strainses such as raw No. 2 common
Activate in pda culture medium；Then use the card punch of diameter 5mm, in colony edge punching, make that cell age is identical, size identical bacterium
Block is seeded in potato full-powder culture medium of the present invention (numbering d, e, f) and difco respectivelytmThe central authorities of pda culture medium, with sealing
Film is sealed up, 3 repetitions of each bacterial strain.Again they are placed in same incubator and cultivate 7 days under the conditions of 24 DEG C of room temperature, lucifuge.
Observe colony diameter, and take pictures.
Result Pleurotus ostreatus (see Figure 37～Figure 40), Lentinus Edodess (see Figure 41～Figure 44), Auricularia (see Figure 45～Figure 48), Auricularia polytricha (Mout) Sacc.
(see Figure 49～Figure 52), Agaricus bisporuss (see Figure 53～Figure 56), Flammulina velutiper (Fr.) Sing (see Figure 57～Figure 60), Pleurotus eryngii are (see Figure 61～figure
64), 9 kinds of edible fungi such as Pleurotus nebrodensis (see Figure 65～Figure 68), Pholiota nameko (see Figure 69～Figure 72) are cultivated in potato full-powder of the present invention
Energy normal growth on base, and its speed of growth is better than them in difcotmThe speed of growth in pda culture medium.Illustrate edible
Bacterium fast growth in potato full-powder culture medium of the present invention.
In sum, potato full-powder culture medium comprehensive nutrition of the present invention, growth in culture medium of the present invention for the edible fungi
Speed substantially fast growth in existing pda culture medium for the ratio, additionally, potato full-powder culture medium preparation method of the present invention
Simply, preparation speed is fast, cost of manufacture significantly reduces.
Claims (10)
1. application on preparing pda culture medium for the potato full-powder.
2. a kind of potato full-powder culture medium is it is characterised in that contain potato full-powder in described potato full-powder culture medium.
3. potato full-powder culture medium according to claim 2 is it is characterised in that its constituent and its ratio are:
4. potato full-powder culture medium according to claim 3 is it is characterised in that the weight ratio of described potato full-powder
For 13～18g.
5. potato full-powder culture medium according to claim 3 it is characterised in that described glucose weight than for 5～
8g.
6. potato full-powder culture medium according to claim 3 is it is characterised in that the weight of described agar powder is than for 14
～16g.
7. the preparation method of the arbitrary described potato full-powder culture medium of claim 3～6 is it is characterised in that claim according to weight ratio
Take potato full-powder, glucose and agar powder, mix homogeneously, be subsequently adding in container, be proportionally added into water, shake up, seal up envelope
Membrana oralis or kraft paper, 121 DEG C of sterilizing 30min, standby.
8. application on culture funguses for the arbitrary described potato full-powder culture medium of claim 1～6.
9. application according to claim 8 is it is characterised in that described funguses refer to yeast, mycete or edible fungi.
10. application according to claim 8 is it is characterised in that described edible fungi refers to Lentinus Edodess, Pleurotus ostreatus, Auricularia, hair
Auricularia, Flammulina velutiper (Fr.) Sing, Agaricus bisporuss, Pleurotus nebrodensis, Pleurotus eryngii, Pleurotus ferulae Lanzi or Pholiota nameko.
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| CN109362487A (en) * | 2018-12-12 | 2019-02-22 | 吕梁学院 | A kind of oyster cap fungus culture medium and preparation method thereof |
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| CN107048350A (en) * | 2017-04-06 | 2017-08-18 | 湖北大学知行学院 | The nutrition full power and its production method of a kind of hericium erinaceus fruiting body implantation potato |
| CN107048350B (en) * | 2017-04-06 | 2021-02-12 | 湖北大学知行学院 | Nutritional whole powder prepared by implanting hericium erinaceus sporocarp into potato and production method thereof |
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| CN109362487A (en) * | 2018-12-12 | 2019-02-22 | 吕梁学院 | A kind of oyster cap fungus culture medium and preparation method thereof |
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