CN106366185A - Device suitable for industrial egg yolk antibody preextraction - Google Patents
Device suitable for industrial egg yolk antibody preextraction Download PDFInfo
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- CN106366185A CN106366185A CN201610759138.9A CN201610759138A CN106366185A CN 106366185 A CN106366185 A CN 106366185A CN 201610759138 A CN201610759138 A CN 201610759138A CN 106366185 A CN106366185 A CN 106366185A
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- tank body
- tank
- interlayer
- stirring paddle
- valve
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000002969 egg yolk Anatomy 0.000 title claims abstract description 70
- 108010000912 Egg Proteins Proteins 0.000 title abstract description 8
- 102000002322 Egg Proteins Human genes 0.000 title abstract description 8
- 235000013345 egg yolk Nutrition 0.000 title abstract description 7
- 239000011229 interlayer Substances 0.000 claims abstract description 67
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 42
- 239000012530 fluid Substances 0.000 claims abstract description 25
- 230000008878 coupling Effects 0.000 claims abstract description 17
- 238000010168 coupling process Methods 0.000 claims abstract description 17
- 238000005859 coupling reaction Methods 0.000 claims abstract description 17
- 230000001105 regulatory effect Effects 0.000 claims abstract description 14
- 238000012806 monitoring device Methods 0.000 claims abstract description 12
- 238000003756 stirring Methods 0.000 claims description 61
- 230000008676 import Effects 0.000 claims description 58
- 239000007788 liquid Substances 0.000 claims description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 33
- 239000007921 spray Substances 0.000 claims description 26
- 238000003860 storage Methods 0.000 claims description 21
- 239000010410 layer Substances 0.000 claims description 18
- 229910000831 Steel Inorganic materials 0.000 claims description 16
- 239000010959 steel Substances 0.000 claims description 16
- 239000000284 extract Substances 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 15
- 239000007787 solid Substances 0.000 claims description 10
- 229910001220 stainless steel Inorganic materials 0.000 claims description 10
- 239000010935 stainless steel Substances 0.000 claims description 10
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 4
- 230000005499 meniscus Effects 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 18
- 238000000605 extraction Methods 0.000 abstract description 10
- 230000008569 process Effects 0.000 abstract description 7
- 230000008901 benefit Effects 0.000 abstract description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 abstract 1
- 229910052739 hydrogen Inorganic materials 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 abstract 1
- 238000002604 ultrasonography Methods 0.000 abstract 1
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 20
- 239000000243 solution Substances 0.000 description 11
- 229960002446 octanoic acid Drugs 0.000 description 10
- 210000000214 mouth Anatomy 0.000 description 8
- 230000001954 sterilising effect Effects 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 description 6
- 238000012544 monitoring process Methods 0.000 description 5
- OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 238000005260 corrosion Methods 0.000 description 4
- 230000007797 corrosion Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000013049 sediment Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 208000031295 Animal disease Diseases 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000011091 antibody purification Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 235000021472 generally recognized as safe Nutrition 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 206010002198 Anaphylactic reaction Diseases 0.000 description 1
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000027312 Bursal disease Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 206010019799 Hepatitis viral Diseases 0.000 description 1
- 206010053425 Injection site swelling Diseases 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000036783 anaphylactic response Effects 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000003816 axenic effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 108010052522 livetin Proteins 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 201000001862 viral hepatitis Diseases 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000013316 zoning Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/02—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from eggs
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Sampling And Sample Adjustment (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
The invention belongs to the egg yolk antibody extraction field, and particularly discloses a device suitable for industrial egg yolk antibody preextraction. The device comprises a tank body I and an external subsystem thereof, and a tank body II and an external subsystem thereof, wherein the external subsystem of the tank body I comprises a tank exterior interlayer temperature regulating device I, and the external subsystem of the tank body II comprises a tank exterior interlayer temperature regulating device II, a tank interior fluid density ultrasound monitoring device, a tank body air pressure power device and a PH (potential of hydrogen) coupling reagent output device. The device suitable for the industrial egg yolk antibody preextraction has the advantages of being precise in structure, and sturdy and durable, facilitating large scale process standardization, achieving continuous operation, significantly improving preextraction efficiency of egg yolk antibodies, and reducing labor intensity.
Description
Technical field
The invention belongs to yolk antibody produces field and in particular to a kind of dress being suitable to extract before industrialization yolk antibody
Put.
Background technology
Yolk antibody (egg yolk antibodies, igy) is the immunoglobulin being present in birdss yolk, refers to
Igy is extracted from egg yolk by the specific antibody that laying hen produces and shifts and be stored in yolk after being subject to specific antigen immunity
And apply and be widely recognized in the preventing and treating of Animal diseases.Due to its have good therapeutic effect, higher specificity,
Safe noresidue, the advantage such as drug resistance bacterium and the big, process is simple of preparation amount that do not produce are in the research boom of Substitutes For Antibiotic
Show one's talent.Since being found first by klemperer from 1898, it has been widely used to the preventing and treating of livestock and poultry and disease is examined
The aspect such as disconnected.Yolk antibody is listed in " generally recognized as safe uses material by United States food and drag administration (fda)
(generally recognized as safe, gras) ", regards efficacy nutritive product as yolk antibody
(nutraceuticals).Generally, yolk antibody does not have toxic and side effects to animal, with yolk antibody treatment Animal diseases not
But avoid the shortcoming that serum is expensive, yield poorly, and the side effect that serum can be avoided to cause over the course for the treatment of, it is
A kind of up-and-coming invention immunoglobulin preparation.Therefore, yolk antibody is in the application of diseases of bird and livestock diagnosis, treatment and prevention
More and more.
But the premise that igy is widely used is how igy substantial amounts of in yolk to be extracted, to be purified.In recent years, domestic
Outer scholar reports the preparation method of many yolk antibodies, but improper, the particularly mesh of the selection due to extraction equipment and use
Front yolk antibody is produced to lack and is applied to scale, industrialization and standardized antibody producing corollary apparatus and so that antibody carries
Take and lead to antibody moiety to lose with later-period purification process, potency reduces, and its result is: on the one hand, causes antibody extraction cost
Improve；On the other hand, cause antibody injections difficult, be difficult to absorb, injection site swelling, anaphylaxiss serious, directly result in anti-
Body therapeutic effect is not good, has a strong impact on the using effect of antibody, significantly limit application in diseases prevention and treatment for the yolk antibody.
Content of the invention
For overcoming the shortcomings of that in existing yolk antibody extraction and industrialization continuous flow procedure, the purpose of the present invention is intended to carry
For a kind of device being suitable to and extracting before industrialization yolk antibody.
For achieving the above object, the technical scheme that the present invention takes is as follows:
A kind of device being suitable to extract before industrialization yolk antibody, this device includes tank body i(and for high-immunity yolk dilution and disappears
Poison) and its extraneous subsystem, tank body ii(are used for the acidifying layering of yolk liquid and antibody extracts) and its extraneous subsystem；
Tank body i bottom is provided with outlet plenum i and pedestal i；Tank body i top is provided with blind flange i, and blind flange i is connected with
Mounting flange i, mounting flange i are provided with yolk liquid import, thermometer jack i, lamp switch i, stirring motor i, distilled water in tank
Import, spray liquid import i and high-temperature steam import；It is provided with lamp i(in the tank being connected with lamp switch i in tank in tank body i to be resistant to
High Temperature High Pressure and corrosion) the shaft i that is connected with stirring motor i of the spray head i that is connected with spray liquid import i,
Shaft i lower end is provided with stirring paddle i；Tank body i outer wall is provided with interlayer i, and interlayer i upper and lower ends are respectively equipped with interlayer import i and folder
Layer outlet i, tank body i outer wall is simultaneously provided with endoscope i, and endoscope i one end is located in interlayer i, the other end stretches out interlayer i；
The extraneous subsystem of tank body i includes tank outside sandwich temperature regulating device i, and described tank outside sandwich temperature regulating device i includes pump i and perseverance
Reservoir i(controllable temperature: 50 ~ 90 DEG C), interlayer outlet i, thermostatic water tank i, pump i, interlayer import i are contacted successively by pipeline and are formed
One closed cycle system；
Tank body ii bottom is provided with outlet plenum ii and pedestal ii；Tank body ii top is provided with blind flange ii, and blind flange ii coordinates
Be connected with mounting flange ii, mounting flange ii be provided with charging aperture, thermometer jack ii, lamp switch ii in tank, stirring motor ii,
Gas circuit mouth, spray liquid import ii and ph coupling reagent liquid import；It is provided with the tank being connected with lamp switch ii in tank in tank body ii
Interior lamp ii(can high temperature high voltage resistant and corrosion) the spray head ii that is connected with spray liquid import ii and stirring motor ii cooperation
The shaft ii connecting, shaft ii lower end is provided with stirring paddle ii；Tank body ii outer wall is provided with interlayer ii, and interlayer ii upper and lower ends are divided
It is not provided with interlayer import ii and interlayer outlet ii, tank body ii outer wall is simultaneously provided with endoscope ii, endoscope ii one end is located at folder
Layer ii is interior, the other end stretches out interlayer ii；
The extraneous subsystem of tank body ii includes tank outside sandwich temperature regulating device ii, fluid density ultrasonic monitoring device, tank body in tank
Pneumatic propelling plant and ph coupling reagent output device；
Described tank outside sandwich temperature regulating device ii includes pump ii and thermostatic water tank ii(controllable temperature: 0 ~ 37 DEG C), interlayer outlet ii, constant temperature
Water tank ii, pump ii, interlayer import ii are contacted successively by pipeline and form a closed cycle system；
In described tank, fluid density ultrasonic monitoring device includes ultrasonic transmitter-receiver, reflecting plate, signal adapter, time continue
Electrical equipment and electromagnetic valve, ultrasonic transmitter-receiver and reflecting plate are located at opposite sides in the middle part of outlet plenum ii, and electromagnetic valve is located at outlet
Chamber ii end, ultrasonic transmitter-receiver is contacted signal adapter, the time relay and electromagnetic valve successively by circuit, electromagnetic valve
Then be connected to storage tank i(by two bye-passes again and be used for storing yolk antibody and extract waste by-product) and storage tank ii(be used for
Extracting solution before storage yolk antibody), the pipeline that electromagnetic valve connects storage tank i is provided with valve i, and electromagnetic valve connects the pipeline of storage tank ii
It is provided with valve ii；
Described tank body Pneumatic propelling plant includes vacuum pump, manostat, pressure gauge, valve iii and air filter, and vacuum pump leads to
Cross pipeline and contact manostat, pressure gauge, valve iii and air filter successively to gas circuit mouth；
Described ph coupling reagent output device include ph sensor, ph controller, constant flow pump, effusion meter i, valve iv, valve v,
Reagent pot i and reagent pot ii, ph sensor is located on tank body ii outer wall, and ph sensor one end is located in interlayer ii, and the other end is stretched
Contacted successively ph controller, constant flow pump by circuit after going out interlayer ii, the input of constant flow pump is connected respectively by two bye-passes
Reagent pot i(stores acidifying solution) and reagent pot ii(storage caprylic acid reagent solution), the pipeline that reagent pot i connects constant flow pump is provided with
The pipeline that valve iv, reagent pot ii connect constant flow pump is provided with valve v, and the outfan of constant flow pump is then contacted flow by pipeline again
Meter i to ph coupling reagent liquid import；
Between tank body i and tank body ii, check-valves, effusion meter ii to charging aperture realization are contacted successively even by pipeline by outlet plenum i
Connect.
Preferably, between blind flange i and mounting flange i, it is equipped with pad between blind flange ii and mounting flange ii
And realize being connected by bolt hole and bolt.
Preferably, tank body i is circle tube tank body, and material is rustless steel, heatproof >=121 DEG C, pressure >=2 atmospheric pressures,
The blade diameter length ratio scope of tank body i is 1/5 ~ 1/3, and bottom is pyramidal structure, and angle of taper is 160 degree；Tank body ii is circle tube tank body,
Material is rustless steel, heatproof >=121 DEG C, pressure >=2 atmospheric pressures, and the blade diameter length ratio scope of tank body ii is 1/6 ~ 1/4, and bottom is
Pyramidal structure, angle of taper is 120 degree.
Preferably, outlet plenum ii cavity internal diameter is the 1/6 ~ 1/5 of tank body ii internal diameter, and outlet plenum ii cavity length is outlet plenum
2 ~ 2.5 times of ii cavity internal diameter.
Preferably, stirring paddle i and stirring paddle ii material are hard rustless steel and in meniscus shaped.
Preferably, if dried layer stirring paddle is symmetrically connected with by stainless steel solid pole on the shaft i above stirring paddle i
I', stirring paddle i' material is hard rustless steel, is shaped as rectangle and surface is evenly distributed with mesh, and mesh aperture is 3 ~ 8mm.
Preferably, the shaft i above stirring paddle i is symmetrically connected with two-layer stirring paddle by stainless steel solid pole
i'.
Preferably, if dried layer stirring is symmetrically connected with by stainless steel solid pole on the shaft ii above stirring paddle ii
Oar ii', stirring paddle ii' material is hard rustless steel, is shaped as rectangle.
Preferably, the shaft ii above stirring paddle ii is symmetrically connected with one layer of stirring paddle by stainless steel solid pole
ii'.
Preferably, the filter opening aperture of air filter is 0.2 ~ 0.22 μm.
Octanoic acid dissolubility extremely low (0.062g/l) in water, therefore in water-soluble substanceses, sad residual quantity is atomic.In doctor
Learn octanoic acid in industry to be widely used, do not have toxic and side effects to biology.Sad method is the igy extraction side being widely used at present
Method.The principle of sad method is to be carried out using the special chemical property of igy: under low ionic strength, ph value acid condition, octanoic acid
Can react with most of livetin, form irreversible precipitation, only igy does not react, and stays supernatant
In, it is centrifuged off precipitating, collect supernatant, then carry out follow-up antibody purification and preparation.
Apparatus of the present invention are exactly the technological requirement taking yolk antibody according to sad legal system, for extraction process before its antibody
Feature and design, fully meeting the item that industrialization carries out in extraction process before yolk antibody requires, be suitable to duck viral hepatitiss,
The extraction of the yolk antibodies such as jaundice viral disease, newcastle, bursal disease.
Beneficial effect:
1st, apparatus of the present invention have precise structure, sturdy and durable, the scale process standard of being easy to operates with, can serialization behaviour
Make, significantly improve extraction efficiency before yolk antibody, reduce the feature of labor intensity；
2nd, apparatus of the present invention also have the advantages that sealing is high, reduce external microbe or impurity pollution, guarantee product quality, with
When this device characteristic (sterilization of yolk automatic dilution, the transmission of fluid sterility air pressure, fluid density on-line monitoring, ph coupling reagent
Output) pole be beneficial to yolk antibody industrialization run；
3rd, in tank body ii, collect same tank body cool down in place, in place sterilizing, octanoic acid automatically add, temperature automatically controlled, mix and blend,
AUTOMATIC ZONING goes out that liquid etc. is multi-functional to improve antibody extraction ratio in one, and in later stage antibody purification procedures, antibody loss is few, promotes ovum
Yellow antibody extracts the standardization producing, and reduces the product quality shadow of the different batches caused by configuration of different size tank body
Ring；
4th, the technique productions application that apparatus of the present invention are extracted before being suitable to industrialization yolk antibody.Therefore, the design of apparatus of the present invention
Seem that particularly there is meaning for the work extracted before industrialization yolk antibody.
Brief description
The structural representation of Fig. 1: apparatus of the present invention；
Fig. 2: the overlooking the structure diagram of mounting flange i；
Fig. 3: the overlooking the structure diagram of mounting flange ii；
Description of reference numerals: 1- tank body i；1'- tank body ii；2- outlet plenum i；2'- outlet plenum ii；3- pedestal i；3'- pedestal ii；4-
Blind flange i；4'- blind flange ii；5- mounting flange i；5'- mounting flange ii；6- pad i；6'- pad ii；7- bolt hole
i；7'- bolt hole ii；8- bolt i；8'- bolt ii；9- yolk liquid import；101- thermometer jack i；101'- thermometer jack
ii；102- thermometer i；102'- thermometer ii；Lamp switch i in 11- tank；Lamp switch ii in 11'- tank；12- stirring motor i；
12'- stirring motor ii；13- distills water inlet；14- spray liquid import i；14'- spray liquid import ii；15- high-temperature steam import；
Lamp i in 16- tank；Lamp ii in 16'- tank；17- spray head i；17'- spray head ii；18- shaft i；18'- shaft ii；19- stirs
Mix oar i；19'- stirring paddle ii；20- stirring paddle i'；20'- stirring paddle ii'；21- interlayer i；21'- interlayer ii；22- interlayer import
i；22'- interlayer import ii；23- interlayer exports i；23'- interlayer exports ii；24- endoscope i；24'- endoscope ii；25- pump i；
25'- pump ii；26- thermostatic water tank i；26'- thermostatic water tank ii；27- charging aperture；28- gas circuit mouth；29-ph coupling reagent liquid import；
30- ultrasonic transmitter-receiver；31- reflecting plate；32- signal adapter；The 33- time relay；34- electromagnetic valve；35- storage tank i；
35'- storage tank ii；361- valve i；362- valve ii；363- valve iii；364- valve iv；365- valve v；37- vacuum pump；
38- manostat；39- pressure gauge；40- air filter；41-ph sensor；42-ph controller；43- constant flow pump；44- effusion meter
i；44'- effusion meter ii；45- reagent pot i；45'- reagent pot ii；46- check-valves.
Specific embodiment
With reference to the accompanying drawings and detailed description present disclosure is further described:
As shown in Fig. 1 ~ 3, a kind of device being suitable to extract before industrialization yolk antibody, this device includes tank body i 1(and exempts from for height
Yolk dilutes and sterilizes) and its extraneous subsystem, tank body ii 1'(are used for, and the acidifying of yolk liquid is layered and antibody extracts) and its outer
Boundary's subsystem；Tank body i 1 is circle tube tank body, and material is rustless steel, heatproof >=121 DEG C, pressure >=2 atmospheric pressures, tank
The blade diameter length ratio scope of body i 1 is 1/4, and bottom is pyramidal structure, and angle of taper is 160 degree；Tank body ii 1' is circle tube tank body,
Material is rustless steel, heatproof >=121 DEG C, pressure >=2 atmospheric pressures, and the blade diameter length ratio scope of tank body ii 1' is 1/4, and bottom is
Pyramidal structure, angle of taper is 120 degree；
Tank body i 1 bottom is provided with outlet plenum i 2 and pedestal i 3；Tank body i 1 top is provided with blind flange i 4, blind flange i 4
It is provided with mounting flange i 5, between blind flange i 4 and mounting flange i 5, be provided with pad i 6, blind flange i 4 and fixation
Four bolt hole i 7 are evenly equipped with blue i 5, pass through bolt i 8 between the two and realize being connected, mounting flange i 5 is provided with
Be inserted with thermometer i 102 in yolk liquid import 9, thermometer jack i 101(), lamp switch i 11 in tank, stirring motor i 12,
Distillation water inlet 13, spray liquid import i 14 and high-temperature steam import 15；It is provided with tank body i 1 and coordinate with lamp switch i 11 in tank
In the tank connecting, lamp i 16(can high temperature high voltage resistant and corrosion) the spray head i 17 that is connected with spray liquid import i 14 and
The shaft i 18 that stirring motor i 12 is connected, shaft i 18 lower end is provided with the hard rustless steel stirring paddle in meniscus shaped
On the shaft i 18 of i 19, stirring paddle i 19 top, two-layer stirring paddle i'20 is symmetrically connected with by stainless steel solid pole,
Stirring paddle i'20 material is hard rustless steel, is shaped as rectangle and surface is evenly distributed with mesh, and mesh aperture is 5mm；Tank
Body i 1 outer wall is provided with interlayer i 21, and interlayer i 21 upper and lower ends are respectively equipped with interlayer import i 22 and interlayer outlet i 23, tank body
Endoscope i 24 is simultaneously provided with i 1 outer wall, endoscope i 24 one end is located in interlayer i 21, the other end stretches out interlayer i 21；
The extraneous subsystem of tank body i 1 includes tank outside sandwich temperature regulating device i, and described tank outside sandwich temperature regulating device i includes pump i
25 and thermostatic water tank i 26(controllable temperature: 50 ~ 90 DEG C), interlayer outlet i 23, thermostatic water tank i 26, pump i 25, interlayer import i
22 are contacted successively by pipeline forms a closed cycle system；
Tank body ii 1' bottom is provided with outlet plenum ii 2' and pedestal ii 3', and outlet plenum ii 2' cavity internal diameter is in tank body ii 1'
The 1/5 of footpath, outlet plenum ii 2' cavity length is 2 times of outlet plenum ii 2' cavity internal diameter；Tank body ii 1' top is provided with sealing method
Blue ii 4', blind flange ii 4' are provided with mounting flange ii 5', are provided between blind flange ii 4' and mounting flange ii 5'
It is evenly equipped with four bolt hole ii 7' on pad ii 6', blind flange ii 4' and mounting flange ii 5', pass through spiral shell between the two
Bolt ii 8' realizes being connected, and mounting flange ii 5' is provided with charging aperture 27, is inserted with temperature in thermometer jack ii 101'(
Degree table i 102'), lamp switch ii 11', stirring motor ii 12', gas circuit mouth 28, spray liquid import ii 14' and ph coupling in tank
Reagent solution import 29；It is provided with tank body ii 1' that in the tank being connected with lamp switch ii 11' in tank, lamp ii 16'(can be high temperature resistant
High pressure and corrosion) the spray head ii 17' and stirring motor ii 12' that is connected with spray liquid import ii 14' is connected
Shaft ii 18', shaft ii 18' lower end is provided with hard rustless steel stirring paddle ii 19', stirring paddle ii in meniscus shaped
On shaft ii 18' above 19', one layer of stirring paddle ii'20', stirring paddle are symmetrically connected with by stainless steel solid pole
Ii'20' material is hard rustless steel, is shaped as rectangle；Tank body ii 1' outer wall is provided with interlayer ii 21', on interlayer ii 21'
Under be respectively arranged at two ends with interlayer import ii 22' and interlayer outlet ii 23', tank body ii 1' outer wall is simultaneously provided with endoscope ii
24', endoscope ii 24' one end is located in interlayer ii 21', the other end stretches out interlayer ii 21'；
The extraneous subsystem of tank body ii 1' includes tank outside sandwich temperature regulating device ii, fluid density ultrasonic monitoring device, tank in tank
Body Pneumatic propelling plant and ph coupling reagent output device；
Described tank outside sandwich temperature regulating device ii includes pump ii 25' and thermostatic water tank ii 26'(controllable temperature: 0 ~ 37 DEG C), interlayer goes out
Mouth ii 23', thermostatic water tank ii 26', pump ii 25', interlayer import ii 22' are contacted successively by pipeline and form a closed circulation
System；
In described tank fluid density ultrasonic monitoring device include ultrasonic transmitter-receiver 30, reflecting plate 31, signal adapter 32,
The time relay 33 and electromagnetic valve 34, ultrasonic transmitter-receiver 30 and reflecting plate 31 are located at relative two in the middle part of outlet plenum ii 2'
Side, electromagnetic valve 34 is located at outlet plenum ii 2' end, and ultrasonic transmitter-receiver 30 is contacted signal adapter successively by holding wire
32nd, the time relay 33 and electromagnetic valve 34, electromagnetic valve 34 is then connected to storage tank i 35(by two bye-passes again and is used for storing
Yolk antibody extract waste by-product) and storage tank ii 35'(be used for storing extracting solution before yolk antibody), electromagnetic valve 34 connect store up
The pipeline of tank i 35 is provided with valve i 361, and the pipeline that electromagnetic valve 34 connects storage tank ii 35' is provided with valve ii 362；
Described tank body Pneumatic propelling plant includes vacuum pump 37, manostat 38, pressure gauge 39, valve iii 363 and air filtration
Device 40(filter opening aperture is 0.2 μm), vacuum pump 37 is contacted manostat 38, pressure gauge 39, valve iii 363 and successively by pipeline
Air filter 40 is to gas circuit mouth 28；
Described ph coupling reagent output device includes ph sensor 41, ph controller 42, constant flow pump 43, effusion meter i 44, valve
Iv 364, valve v 365, reagent pot i 45 and reagent pot ii 45', ph sensor 41 is located on tank body ii 1' outer wall, and ph passes
Sensor 41 one end is located in interlayer ii 21', and the other end is contacted ph controller successively by holding wire after stretching out interlayer ii 21'
42nd, constant flow pump 43, the input of constant flow pump 43 connects reagent pot i 45(respectively by two bye-passes and stores acidifying solution) and reagent
Tank ii 45'(stores caprylic acid reagent solution), the pipeline that reagent pot i 45 connects constant flow pump 43 is provided with valve iv 364, reagent
The pipeline that tank ii 45' connects constant flow pump 43 is provided with valve v 365, and the outfan of constant flow pump 43 is then contacted stream by pipeline again
Gauge i 44 to ph coupling reagent liquid import 29；
Between tank body i 1 and tank body ii 1', check-valves 46, effusion meter ii 44' are contacted successively extremely by pipeline by outlet plenum i 2
Charging aperture 27 is realized connecting.
Work process:
1. first in gnotobasiss, the egg that height is exempted from being carried out shell sterilization recycles yolk separator to obtain without other miscellaneous
The complete yolk of matter, by the yolk of acquisition, in sterile plastic container, simple agitation processes yolk and makes yolk liquid standby；
2. valve i 361, valve ii 362, valve iii 363, valve iv 364, valve v 365 and mounting flange i 5 are closed
And all imports on mounting flange ii 5', open high-temperature steam import 15, make high temperature sterilize steam be introduced into tank body i 1, after
Through outlet plenum i 2, pipeline, tank body ii 1' is entered by charging aperture 27, and then carry out tank body i 1, tank body ii 1' and pipeline sterilizing work
Sequence；
3., after sterilization process, close outlet plenum i 2 when tank body i 1 tank temperature drops to 60 ~ 70 DEG C, by operation before tank body i 1
Pump provide power that through yolk liquid import 9 yolk liquid is input into tank body i 1, input finishes closing yolk liquid import 9；
4. still close outlet plenum i 2, open stirring motor i 12 and pump i 25 simultaneously, stirring motor i 12 drives shaft i 18
It is stirred, (ph value is aseptic for 4.0 ~ 6.0 for the synchronous faintly acid distilled water by certain volume (yolk liquid amass 3 ~ 5 times)
Distilled water) through, in distillation water inlet 13 input tank body i 1, stir speed (S.S.) is by slow-to-fast (90 rpm/min → 110 rpm/min)
Setting speed, pump i 25 synchronous will thermostatic water tank i 26 be heated to the hot water of predetermined set temperature (65 ~ 70 DEG C) through pipeline by
Interlayer import i 22 inputs into interlayer i 21, then exports i 23 through defeated time thermostatic water tank i 26 of pipeline by interlayer, controls a timing
Between (30 ~ 40min) and then reach the disinfective action of yolk diluent；
5. first open pump ii 25', pump ii 25' will freeze to predetermined set temperature (30 ~ 37 DEG C) in thermostatic water tank ii 26'
Warm water is inputted into interlayer ii 21' by interlayer import ii 22' through pipeline, then by interlayer outlet ii 23' through defeated time thermostatted water of pipeline
Case ii 26'.In tank body ii 1', temperature drops to closing pump ii 25' during technological requirement (30 ~ 37 DEG C), opens valve iii
363 and start vacuum pump 37, whne tank body ii 1' upper end pressure gauge 39 pointer point to high negative pressure when open going out of tank body ii 1'
Oral cavity ii 2', slightly opens yolk liquid import 9, and the yolk diluent after above-mentioned process just passes through charging by outlet plenum i 2 through pipeline
In mouth 27 input tank body ii 2'；
6. close vacuum pump 37, valve iii 363, yolk liquid import 9, outlet plenum i 2 and charging aperture 27, open stirring motor ii
12' and constant flow pump 43, open valve iv 364, shaft ii 18' uniform rotation, (1mol/l hydrochloric acid is adjusted acidifying solution
Axenic purification water, acidifying solution end ph value is 3.2 ~ 4.0) ph coupling reagent liquid import 29 constant speed is passed through through pipeline by reagent pot i 45
In input tank body ii 1', when liquid ph value to be mixed reaches setting value (5.0 ~ 5.5), signal is reached ph control by ph sensor 41
Device 42, through signal line traffic control and cuts out constant flow pump 43 by ph controller 42, cuts out valve iv 364 and stirring motor ii 12'；Again
Open pump ii 25', the cold water that pump ii 25' will freeze to predetermined set temperature (3 ~ 5 DEG C) in thermostatic water tank ii 26' is through pipeline
Inputted into interlayer ii 21' by interlayer import ii 22', then by interlayer outlet ii 23' through defeated time thermostatic water tank ii 26' of pipeline,
The temperature in tank body ii 1' that makes constantly regulate pump ii 25' continues to drop to the temperature required value of technique (3 ~ 5 DEG C) and both keep technique
Fix time (12 ~ 20h)；
7. the fluid in tank body ii 1' at the end of keeping technique given time has completed layering, and (lower floor is precipitation, upper strata is supernatant
Liquid), start tank in fluid density ultrasonic monitoring device, in tank fluid density ultrasonic monitoring device can on-line monitoring arrive be layered stream
The difference of body density, then signal adapter 32 different instruction will be converted into through the time relay according to the unlike signal that monitors
33 automatically control electromagnetic valve 34 and then automatically control different densities fluid from outlet plenum ii 2' end outflow tank body ii 1'；First
Open valve i 361, in tank, fluid density ultrasonic monitoring device can on-line monitoring make to control electricity to lower sediment segment fluid flow
Magnet valve 34 and then complete this fluid and enter storage tank i 35 through pipeline to carry out the work of interim storage, treats that lower sediment segment fluid flow is arranged
After going out tank body ii 1', electromagnetic valve 34 can accept instruction and cut out in time, close valve i 361 it is ensured that supernatant must not be discharged；
8. open stirring motor ii 12' and constant flow pump 43, open valve v 365, shaft ii 18' uniform rotation, caprylic acid
Reagent solution (the caprylic acid aqueous solution for concentration of volume percent 0.35 ~ 0.39%；This caprylic acid aqueous solution can be dissolved by caprylic acid
40 ~ 50 DEG C of warm water is prepared from) input of ph coupling reagent liquid import 29 constant speed is passed through through pipeline by reagent pot ii 45'
In tank body ii 1', when in tank body ii 1', supernatant ph value reaches setting value (4.0 ~ 4.5), signal is reached by ph sensor 41
Ph controller 42, through signal line traffic control and cuts out constant flow pump 43 by ph controller 42, cuts out valve v 365 and stirring motor ii
12' is static according to the technological requirement time (6 ~ 10h)；At the end for the treatment of technique set quiescent time, the fluid in tank body ii 1' is again
Secondary clearing (lower floor is precipitation, middle level is supernatant, top layer is float), starts fluid density ultrasonic monitoring device in tank, tank
Interior fluid density ultrasonic monitoring device can the fluid density to layering for the on-line monitoring difference, then by signal adapter 32 by root
It is converted into different instruction according to the unlike signal monitoring to automatically control electromagnetic valve 34 through the time relay 33 and then automatically control not
Flow out tank body ii 1' with density fluid from outlet plenum ii 2' end, lower sediment segment fluid flow discharges the operation of tank body ii 1'
With reference to 7., first close valve i 361 after lower sediment segment fluid flow is discharged and before the discharge of middle layer supernatant liquid and open valve ii
362, and then layer fluid smoothly enters storage tank ii 35' and is stored that (this step has completed to extract before yolk antibody temporarily in ensureing
Operation)；Same top layer float enters storage tank i 35 also dependent on same principle through pipeline and carries out interim storage；
9. two tank body cleanings and sterilizing: during two tank bodies cleanings, open spray liquid import i 14 and spray liquid import ii 14', will be clean
Water purification enters two tank bodies through spray head i 17 and spray head ii 17' and is carried out, and at the end for the treatment of two tank body cleanings, opens tank body
Pneumatic propelling plant, by the washings input tank body ii 1' in tank body i 1, is then turned on electromagnetic valve 34 by tank body ii 1' wash-in
Wash water to be discharged through pipeline input storage tank i 35；Carry out high-temp steam sterilizing after two tank body cleanings terminate, operational approach is same
②.
Last it is noted that above example is only in order to illustrate technical scheme and unrestricted, although reference
Preferred embodiment has been described in detail to the present invention, it will be understood by those within the art that, can be to the present invention's
Technical scheme is modified or equivalent, and without deviating from the spirit and scope of technical solution of the present invention, it all should be covered
In the middle of scope of the presently claimed invention.
Claims (10)
1. a kind of be suitable to industrialization yolk antibody before the device that extracts it is characterised in that: this device includes tank body i and its extraneous attached
Genus system, tank body ii and its extraneous subsystem；
Tank body i bottom is provided with outlet plenum i and pedestal i；Tank body i top is provided with blind flange i, and blind flange i is connected with
Mounting flange i, mounting flange i are provided with yolk liquid import, thermometer jack i, lamp switch i, stirring motor i, distilled water in tank
Import, spray liquid import i and high-temperature steam import；It is provided with lamp i and spray in the tank being connected with lamp switch i in tank in tank body i
The shaft i that the spray head i that pouring liquid import i is connected is connected with stirring motor i, shaft i lower end is provided with stirring paddle
i；Tank body i outer wall is provided with interlayer i, and interlayer i upper and lower ends are respectively equipped with interlayer import i and interlayer outlet i, same on tank body i outer wall
When be provided with endoscope i, endoscope i one end be located at interlayer i in, the other end stretch out interlayer i；
The extraneous subsystem of tank body i includes tank outside sandwich temperature regulating device i, and described tank outside sandwich temperature regulating device i includes pump i and perseverance
Reservoir i, interlayer outlet i, thermostatic water tank i, pump i, interlayer import i are contacted successively by pipeline and form a closed cycle system；
Tank body ii bottom is provided with outlet plenum ii and pedestal ii；Tank body ii top is provided with blind flange ii, and blind flange ii coordinates
Be connected with mounting flange ii, mounting flange ii be provided with charging aperture, thermometer jack ii, lamp switch ii in tank, stirring motor ii,
Gas circuit mouth, spray liquid import ii and ph coupling reagent liquid import；It is provided with the tank being connected with lamp switch ii in tank in tank body ii
The shaft ii that the spray head ii that interior lamp ii is connected with spray liquid import ii is connected with stirring motor ii, shaft
Ii lower end is provided with stirring paddle ii；Tank body ii outer wall is provided with interlayer ii, and interlayer ii upper and lower ends are respectively equipped with interlayer import ii and folder
Layer outlet ii, tank body ii outer wall is simultaneously provided with endoscope ii, and endoscope ii one end is located in interlayer ii, the other end stretches out interlayer
ii；
The extraneous subsystem of tank body ii includes tank outside sandwich temperature regulating device ii, fluid density ultrasonic monitoring device, tank body in tank
Pneumatic propelling plant and ph coupling reagent output device；
Described tank outside sandwich temperature regulating device ii includes pump ii and thermostatic water tank ii, interlayer outlet ii, thermostatic water tank ii, pump ii, folder
Layer import ii is contacted successively by pipeline and forms a closed cycle system；
In described tank, fluid density ultrasonic monitoring device includes ultrasonic transmitter-receiver, reflecting plate, signal adapter, time continue
Electrical equipment and electromagnetic valve, ultrasonic transmitter-receiver and reflecting plate are located at opposite sides in the middle part of outlet plenum ii, and electromagnetic valve is located at outlet
Chamber ii end, ultrasonic transmitter-receiver is contacted signal adapter, the time relay and electromagnetic valve successively by circuit, electromagnetic valve
Then it is connected to storage tank i and storage tank ii by two bye-passes again, the pipeline that electromagnetic valve connects storage tank i is provided with valve i, electromagnetism
The pipeline that valve connects storage tank ii is provided with valve ii；
Described tank body Pneumatic propelling plant includes vacuum pump, manostat, pressure gauge, valve iii and air filter, and vacuum pump leads to
Cross pipeline and contact manostat, pressure gauge, valve iii and air filter successively to gas circuit mouth；
Described ph coupling reagent output device include ph sensor, ph controller, constant flow pump, effusion meter i, valve iv, valve v,
Reagent pot i and reagent pot ii, ph sensor is located on tank body ii outer wall, and ph sensor one end is located in interlayer ii, and the other end is stretched
Contacted successively ph controller, constant flow pump by circuit after going out interlayer ii, the input of constant flow pump is connected respectively by two bye-passes
The pipeline that reagent pot i and reagent pot ii, reagent pot i connect constant flow pump is provided with valve iv, and reagent pot ii connects the pipe of constant flow pump
Road is provided with valve v, and the outfan of constant flow pump is then contacted effusion meter i to ph coupling reagent liquid import by pipeline again；
Between tank body i and tank body ii, check-valves, effusion meter ii to charging aperture realization are contacted successively even by pipeline by outlet plenum i
Connect.
2. be suitable to the device that extracts before industrialization yolk antibody as claimed in claim 1 it is characterised in that: blind flange i
It is equipped with pad and realize joining by bolt hole and bolt and mounting flange i between, between blind flange ii and mounting flange ii
Close and connect.
3. be suitable to the device that extracts before industrialization yolk antibody as claimed in claim 1 it is characterised in that: tank body i is circle
Tubular tank body, material is rustless steel, heatproof >=121 DEG C, pressure >=2 atmospheric pressures, and the blade diameter length ratio scope of tank body i is 1/5 ~ 1/
3, bottom is pyramidal structure, and angle of taper is 160 degree；Tank body ii is circle tube tank body, and material is rustless steel, heatproof >=121 DEG C,
Pressure >=2 atmospheric pressures, the blade diameter length ratio scope of tank body ii is 1/6 ~ 1/4, and bottom is pyramidal structure, and angle of taper is 120 degree.
4. be suitable to the device that extracts before industrialization yolk antibody as claimed in claim 3 it is characterised in that: outlet plenum ii chamber
Internal footpath is the 1/6 ~ 1/5 of tank body ii internal diameter, and outlet plenum ii cavity length is 2 ~ 2.5 times of outlet plenum ii cavity internal diameter.
5. be suitable to the device that extracts before industrialization yolk antibody as claimed in claim 1 it is characterised in that: stirring paddle i and
Stirring paddle ii material is hard rustless steel and is in meniscus shaped.
6. be suitable to the device that extracts before industrialization yolk antibody as claimed in claim 5 it is characterised in that: on stirring paddle i
If being symmetrically connected with dried layer stirring paddle i' by stainless steel solid pole on the shaft i of side, stirring paddle i' material is for hard not
Rust steel, is shaped as rectangle and surface is evenly distributed with mesh, and mesh aperture is 3 ~ 8mm.
7. be suitable to the device that extracts before industrialization yolk antibody as claimed in claim 6 it is characterised in that: on stirring paddle i
On the shaft i of side, two-layer stirring paddle i' is symmetrically connected with by stainless steel solid pole.
8. be suitable to the device that extracts before industrialization yolk antibody as claimed in claim 5 it is characterised in that: on stirring paddle ii
If being symmetrically connected with dried layer stirring paddle ii' by stainless steel solid pole on the shaft ii of side, stirring paddle ii' material is hard
Rustless steel, is shaped as rectangle.
9. be suitable to the device that extracts before industrialization yolk antibody as claimed in claim 8 it is characterised in that: on stirring paddle ii
On the shaft ii of side, one layer of stirring paddle ii' is symmetrically connected with by stainless steel solid pole.
10. be suitable to the device that extracts before industrialization yolk antibody as claimed in claim 1 it is characterised in that: air filtration
The filter opening aperture of device is 0.2 ~ 0.22 μm.
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| CN201610759138.9A CN106366185B (en) | 2016-08-30 | 2016-08-30 | A kind of device extracted before the Yolk antibody suitable for industrialization |
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|---|---|---|---|
| CN201610759138.9A CN106366185B (en) | 2016-08-30 | 2016-08-30 | A kind of device extracted before the Yolk antibody suitable for industrialization |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201634611U (en) * | 2009-11-11 | 2010-11-17 | 辽宁益康生物制品有限公司 | Yolk acidification and lamination antibody extracting tank |
| CN103509106A (en) * | 2013-10-08 | 2014-01-15 | 河南科技学院 | Method for extracting chicken egg-yolk antibody |
| CN105175536A (en) * | 2015-09-30 | 2015-12-23 | 大连理工大学 | Egg yolk antibody extraction method suitable for large-scale industrial production |
| CN105693854A (en) * | 2016-04-01 | 2016-06-22 | 重庆三杰众鑫生物工程有限公司 | Preparation method of refined yolk antibody against duck tembusu virus |
-
2016
- 2016-08-30 CN CN201610759138.9A patent/CN106366185B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201634611U (en) * | 2009-11-11 | 2010-11-17 | 辽宁益康生物制品有限公司 | Yolk acidification and lamination antibody extracting tank |
| CN103509106A (en) * | 2013-10-08 | 2014-01-15 | 河南科技学院 | Method for extracting chicken egg-yolk antibody |
| CN105175536A (en) * | 2015-09-30 | 2015-12-23 | 大连理工大学 | Egg yolk antibody extraction method suitable for large-scale industrial production |
| CN105693854A (en) * | 2016-04-01 | 2016-06-22 | 重庆三杰众鑫生物工程有限公司 | Preparation method of refined yolk antibody against duck tembusu virus |
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Application publication date: 20170201 Assignee: FUJIAN NINGDE BEIDI BIOTECHNOLOGY CO.,LTD. Assignor: FUJIAN BRADY PHARMACEUTICAL CO.,LTD. Contract record no.: X2023980054574 Denomination of invention: A device suitable for industrial pre extraction of yolk antibodies Granted publication date: 20190405 License type: Common License Record date: 20240304 |
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