CN106366171A - Pilot scale production process and production line of white zein - Google Patents
Pilot scale production process and production line of white zein Download PDFInfo
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- CN106366171A CN106366171A CN201611104803.7A CN201611104803A CN106366171A CN 106366171 A CN106366171 A CN 106366171A CN 201611104803 A CN201611104803 A CN 201611104803A CN 106366171 A CN106366171 A CN 106366171A
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Abstract
The invention discloses a pilot scale production process and production line of white zein. According to the production process, corn protein powder and ethanol water solution are mixed; then, dynamic multi-stage continuous reverse flow ultrasonic extraction is performed; extracts are firstly filtered by a scraper type filter to remove large particle impurity ingredients such as corn protein powder in the extraction solution, are then filtered by a ceramic membrane to remove filter slag of pectin fiber and the like, are next filtered through an ultra-nanofiltration membrane so that micromolecular substances such as maize yellow pigments can be separated from the zein; the zein solution is concentrated through the ultra-nanofiltration membrane, and ethanol solvents are recovered; next, vacuum concentration condensation is performed for recovering the ethanol for a second time; meanwhile, water is added for precipitating and separating out zein wet powder; the zein wet powder is dried and crushed to obtain the white zein product. The produced white zein has high quality, no solvent residue and green and health effects; the added value of the corn protein powder is improved; the integral development and utilization of the corn protein powder are facilitated.
Description
Technical field
The present invention relates to a kind of production technology of white zein and its production line, belong to zein and add
Work technical field.
Background technology
Zein is main storage protein in Semen Maydiss, accounts for the 45% of Semen Maydiss total protein content.It has uniqueness
Dissolubility, water insoluble or anhydrous alcohols, but the alcohol solution of volume fraction 60%～95% can be dissolved in.According to dissolving
Zein is divided into four classes (α, β, γ and δ) by property and the big I of molecular mass.Zein is by special amino
Acid composition, has substantial amounts of hydrophobic amino acid in its molecule, lack the amino of acid, alkaline and polar group that can be powered
Acid, but containing more sulfur-containing amino acid；Its special Amino acid group has been shaped as special functional character, as stronger moisture absorption
Property, thermostability, resistance to lipid and film property.The bioactive peptide produced using it, can be used as the additive of high protein content medical products
Deng being widely used in the industry such as food, medicine, textile and paper.
However, existing industrialization zein produces mainly with Zein powder as raw material, aquiferous ethanol extracts, many
Pigment (beta-carotene, phylloxanthin and zeaxanthin using extraction, while zein is extracted, in raw material
Deng) and smell substance also dissolution therewith, lead to zein finished product to assume yellow, colourity does not reach answering of international market
With requiring, limit the application in food and medicine field for the zein.Comparatively speaking, white zein has
Broader market prospect.Another factor limiting the development of food pharmaceutical grade zein is to produce zein
Required amount of alcohol is big, and is difficult to reclaim, and leads to high cost.
At present, the production technology of existing related patent and document report white zein, but all rest on
Laboratory stage, not yet carries out pilot scale and large-scale production.In our seminar's previous experiments room research and utilization membrane separation techniques
From the method (patent cn201610358358.0) of Zein powder separation and Extraction corn yellow OB and zein, be by
Zein powder is mixed with ethanol water after pulverizing, supersound extraction under the conditions of lucifuge；Centrifugation after supersound extraction finishes obtains
Supernatant；Then supernatant is carried out membrance separation, obtain corn yellow OB solution and zein solution；Corn yellow OB
Solution is corn yellow OB product through one-tenth red oil concentrated in vacuo；Zein solution is through washing, precipitating, do
Dry, the white solid obtaining is zein.In zein product, protein content is calculated as 90% with mass fraction
~ 93%, significantly larger than commercially available domestic zein product (protein content 83.26%)；Zein yield be 40% ~
46%.Patent of the present invention is on the basis of our seminars's previous experiments achievement (patent cn201610358358.0), right further
Ultrasonic extraction device condition is optimized and pilot plant test with factors such as membrane aperture.
Pilot plant test is in order that scientific achievement complies with market and industrialized requirement, reduces conversion risk, improves conversion ratio,
And the batch methods trial production carrying out.The purpose of this process is checking, improves, and improves laboratory result or theoretical result,
Eliminate various uncertain factors, obtain the relevant technique of product, equipment correlation technique reliability data, parameter and its intermediate products
Quality controllable touchstone, is allowed to be matched with other correlation techniques, is consistent with produce reality, with social need phase one
Cause, so that new technique is complied with production, new product is successfully introduced to the market.Pilot plant test is that scientific and technological achievement turns to the productivity
The necessary links changed, the success or failure of achievement industrialization depend primarily on the success or failure of pilot scale, and therefore, pilot plant test is critically important.And for
For production technology and product quality require higher food stage and the zein of pharmaceutical grade, more should set up one
Pilot production line, this has extremely important function and significance for zein large-scale production.
Content of the invention
The purpose of the present invention be for prior art produce zein high cost, product colour partially yellow the problems such as,
A kind of production technology of the white zein that ethanol is carried out with secondary recovery, low production cost is provided；
It is a further object to provide a kind of production line of white zein, to realize the molten egg of White corn alcohol
White large-scale production.
A kind of production technology of white zein that the present invention provides, is by Zein powder and ethanol water
Carry out dynamic multi-level continuous flow upstream supersound extraction under the conditions of lucifuge after mixing；Extract is first through duplex scraper type filter just mistake
Filter the large granular impurity composition such as Zein powder in extraction solution, then remove pectin fiber therein etc. through ceramic membrane filter
Filtering residue, is then made corn yellow OB and small-molecule substance be separated with zein through super nanofiltration membrane, obtains jade
Rice prolamin liquid；Zein liquid passes through super nanofiltration membrance concentration again and reclaims alcohol solvent, then through condensation concentrated in vacuo
Secondary recovery ethanol, the zein wet-milling of aqueous precipitation precipitation simultaneously；Zein wet-milling is vacuum dried, powder
Broken, vacuum packaging, that is, obtain white zein product.
The percentage by volume 60% ~ 95% of ethanol water, Zein powder is 1:3 ~ 1 with the solid-to-liquid ratio of ethanol water:
25kg/l.
Continuous flow upstream ultrasonic extraction process is: using dynamic three-level continuous flow upstream supersound extraction, controls Extracting temperature to be 30
DEG C ~ 60 DEG C, supersonic frequency is 10khz ~ 30khz, and extraction time is 15min ~ 120min；Stir speed (S.S.) is 30 ~ 150 revs/min.
Duplex scraper type filter prefiltration technique is: filter screen mesh number be 60 ~ 120 mesh, filter pressure be -0.02mpa ~
0.1mpa, stir speed (S.S.) is 70 ~ 120 revs/min.
Ceramic membrane filter technique is: the membrane aperture of ceramic membrane filter is 50 ~ 100um, and pressure is 0 ~ 10bar, and temperature is 20
℃~100℃.
Super nanofiltration membrane technique is: super nanofiltration membrane aperture is 10kda ~ 80kda, and pressure is 0 ~ 40bar, and temperature is 20 DEG C
~100℃.
Super NF membrane protein concentration technique is: super nanofiltration membrane aperture is 360da, pressure limit 0 ~ 40bar, and temperature is 20 DEG C
~ 40 DEG C,
Vacuum concentration process is: 30 DEG C ~ 45 DEG C of thickening temperature, pressure be -0.02mpa ~ 0.1mpa, concentration time be 10 min ~
180 min, the alcohol concentration being concentrated into concentrated solution is 40%, and add water precipitation zein.
Drying process is: baking temperature be 30 DEG C ~ 60 DEG C, pressure be 0.2kpa ~ 0.9kpa, drying time be 60 min ~
360 min.
Disintegrating process is: Semen Maydiss alcohol zein powder being ground under the conditions of 20 DEG C ~ 50 DEG C 100 ~ 200 mesh is molten
Egg albumen powder.
Vacuum packaging: package temperature is 20 DEG C ~ 50 DEG C, vacuum pressure is 0.1pa ~ 0.1mpa, pumpdown time 1s ~ 30s,
70 DEG C ~ 90 DEG C of heat-sealing temperature, seals time 1s ~ 10s.
The production technology of corresponding white zein, its pilot production line includes charging system, extraction system, filtration
System, concentration systems, vacuum dryer, pulverizer and vacuum packing machine.Company between the structure to each system and system below
Connect and cooperation is described in detail.
Charging system, including solvent blend tank, the entrance of solvent blend tank is provided with solvent filter, to being added to solvent
The ethanol solution of blend tank is filtered.Online solvent strength analyzer and solvent rheometer are installed in solvent blend tank,
Line solvent strength analyzer is used for measuring the concentration of etoh solvent in solvent blend tank, and solvent rheometer is used for measuring to be adjusted from solvent
Distribution tank is added to the volume amount extracting solvent in circulating tank.Solvent blend tank passes through centrifugal pump by the solvent in solvent blend tank
Pump into extraction system.
Extraction system, including countercurrent extraction tank, is provided with agitating device, is provided with outside countercurrent extraction tank in countercurrent extraction tank
The ultrasound reactor of UNICOM is so that adverse current is extracted in and carries out under ultrasound condition therewith.Ultrasound reactor can adopt energy gathering type
Ultrasound reactor.In order to improve extraction efficiency, countercurrent extraction tank adopts three-level continuous countercurrent extracting tank, and every stage countercurrent extracts
Pass through underflow membrane pump UNICOM, afterbody countercurrent extraction tank is connected with filtration system by underflow membrane pump between tank.
Filtration system include successively scraper type filter, coarse filtration liquid temporary storage tank, purpose ceramic-film filter, ceramic membrane filtrate keep in
Tank, super nano-filtration membrane filter, super nanofiltration liquid albumen temporary storage tank, super nanofiltration liquid pigment temporary storage tank seven part；Wherein scraper type filters
Device is two scraper type filters, and the liquid outlet of scraper type filter pumps into coarse filtration liquid temporary storage tank by underflow membrane pump；Coarse filtration
The liquid of liquid temporary storage tank pumps into purpose ceramic-film filter by underflow membrane pump；The filtrate of purpose ceramic-film filter passes through high pressure centrifugal pump
Pump into ceramic membrane filtrate temporary storage tank；The thing of valve and pipeline and super nano-filtration membrane filter is passed through in the bottom of ceramic membrane filtrate temporary storage tank
Material entrance point connects, and the solution at super nano-filtration membrane filter material inlet end pumps in super nanofiltration membrane component by underflow membrane pump,
Feed liquid is made to enter membrane filtration；The penetrating fluid port of export solution of super nano-filtration membrane filter pumps into super nanofiltration liquid pigment by high pressure centrifugal pump
Temporary storage tank, the feed liquid membrane concentrated solution outlet end solution of super nano-filtration membrane filter pumps into super nanofiltration liquid albumen by underflow membrane pump
Temporary storage tank；Valve is passed through in the bottom of super nanofiltration liquid albumen temporary storage tank and pipeline is connected with super nanofiltration liquid protein concentration equipment.
Concentration systems include super nanofiltration liquid protein concentration equipment, protein concentrated solution temporary storage tank, solvent recovery temporary storage tank, scraper plate
Vacuum concentration equipment, spiral winding pipe condenser, ethanol reclaim temporary storage tank and albumen separates out seven parts of equipment；Wherein super nanofiltration
The penetrating fluid port of export solution of the white concentrator of liquid eggs pumps into solvent recovery temporary storage tank by high pressure centrifugal pump, and its feed liquid membrane is dense
Contracting liquid port of export solution pumps into protein concentrated solution temporary storage tank by underflow membrane pump；Protein liquid in protein concentrated solution temporary storage tank leads to
Cross underflow membrane pump and pump into scraper plate vacuum concentration equipment；The bottom of scraper plate vacuum concentration equipment connects albumen by underflow membrane pump
Precipitation equipment, its top connects spiral winding pipe condenser by pipeline, and the ethanol that spiral winding pipe condenser reclaims is through high pressure
Centrifugal pump pumps into ethanol and reclaims temporary storage tank.
In sum, the present invention compared with prior art has the following characteristics that
1st, the present invention is combined with membrane separation technique using dynamic multi-level continuous flow upstream ultrasound assisted extraction technique and produces the molten egg of Semen Maydiss alcohol
In vain, the zein product color produced is white, and quality height is not so that the application of zein is limited by its color
System, by broader applications in food and pharmaceuticals industry, significantly improves the added value of Zein powder, is conducive to Zein powder
Comprehensive development and utilization；
2, the invention discloses a kind of pilot production line of white zein, have filled up the life of white zein
The blank of producing line.This production line carries out secondary recovery it is achieved that the reusable edible of solvent to the etoh solvent using, it is to avoid
Solvent, to environment, reduces the production cost of zein, makes no molten in Zein powder product simultaneously
Agent remains, green health.
Brief description
Fig. 1 is the topology layout figure of pilot production line charging system of the present invention and extraction system；
Fig. 2 is the topology layout figure of pilot production line extraction system three-level continuous countercurrent extracting tank of the present invention；
Fig. 3 is the topology layout figure of scraper type filter and coarse filtration liquid temporary storage tank in pilot production line filtration system of the present invention；
Fig. 4 is purpose ceramic-film filter, the topology layout figure of ceramic membrane filtrate temporary storage tank in pilot production line filtration system of the present invention；
Fig. 5 is super nano-filtration membrane filter, super nanofiltration liquid albumen temporary storage tank and super nanofiltration in pilot production line filtration system of the present invention
The topology layout figure of liquid pigment temporary storage tank；
Fig. 6 is that in pilot production line concentration systems of the present invention, super nanofiltration liquid protein concentration equipment, super nanofiltration liquid protein concentrated solution are temporary
Deposit the topology layout figure of tank and solvent recovery temporary storage tank；
Fig. 7 is pilot production line concentration systems middle scraper vacuum concentration equipment of the present invention, spiral winding pipe condenser, ethanol reclaim
Temporary storage tank and the topology layout figure of albumen precipitation equipment.
Specific embodiment
Scale up test technique and production line below by the white zein to the present invention for the specific embodiment are made
Further illustrate.
Embodiment 1
(1) solvent blend tank and solvent allotment
The entrance of solvent blend tank 1 is provided with solvent filter, and the ethanol solution being added to solvent blend tank is filtered.Molten
Online solvent strength analyzer and solvent rheometer are installed in matchmaker's blend tank, online solvent strength analyzer is used for measuring solvent
The concentration of etoh solvent in blend tank, solvent rheometer is used for mensure and is added to solvent extraction circulating tank from solvent blend tank
Volume amount.Solvent in solvent blend tank is pumped into extraction system by centrifugal pump 2 by solvent blend tank.
Solvent is allocated: in solvent blend tank, adds ethanol and water, ethanol water is deployed into percentage by volume is
80% solution.
(2) three-level continuous countercurrent extracting tank and its extraction process
Countercurrent extraction tank adopts three-level continuous countercurrent extracting tank 3, and passes through underflow membrane pump 6 UNICOM between every stage countercurrent extraction pot,
Afterbody countercurrent extraction tank is connected with filtration system by underflow membrane pump.In every stage countercurrent extraction pot, agitating device is installed
4, the ultrasound reactor 5 of UNICOM therewith is installed so that adverse current is extracted in and carries out under ultrasound condition outside countercurrent extraction tank.Ultrasonic
Ripple reactor can adopt energy-gathered ultrasonic reactor.
Every grade of extraction process: add Zein powder 15kg in countercurrent extraction tank, according still further to Zein powder and ethanol
Solution pumps into 80% ethanol water 225l with the solid-to-liquid ratio of 1:15kg/l.It is then turned on the stirring of energy-gathered ultrasonic reactor to extract,
Carry out supersound extraction under lucifuge airtight condition, Extracting temperature is 45 DEG C, supersonic frequency is 19.73khz, and extraction time is
60min；Stir speed (S.S.) is 50 revs/min.
(3) scraper type filter and prefiltration
Using two scraper type filters 7, coarse filtration is carried out to the extracting solution of continuous flow upstream supersound extraction tank, molten to remove extraction
The large granular impurity composition such as Zein powder in liquid.The filtrate of the liquid outlet of scraper type filter is pumped into by underflow membrane pump 6
Coarse filtration liquid temporary storage tank 8.
Prefiltration: scraper type strainer screen mesh number is 80 mesh, filter pressure is -0.085mpa, scraper type filter
Scraper speed is 90 revs/min.
(4) purpose ceramic-film filter and filtration
Purpose ceramic-film filter 9 is adopted the liquid in coarse filtration liquid temporary storage tank to be filtered to remove fiber and the fruit extracted in solution
The impurity such as glue.The liquid of coarse filtration liquid temporary storage tank 8 is pumped into purpose ceramic-film filter 9 by underflow membrane pump 6 filtered.Filtrate is led to
Cross high pressure centrifugal pump and pump into ceramic membrane filtrate temporary storage tank 10.
Ceramic membrane filter: the membrane aperture of ceramic membrane filter is 50um, pressure is 2bar, and temperature is 24 DEG C.
(5) super nano-filtration membrane filter and filtration
Using super nano-filtration membrane filter 11, the liquid of ceramic membrane filtrate temporary storage tank 10 is filtered, made pigment and the molten egg of Semen Maydiss alcohol
In vain separately.Open the valve of ceramic membrane filtrate temporary storage tank 10 bottom so as in liquid enter excess of imports nano-filtration membrane filter 11 and carry out
Filter.The liquid of the penetrating fluid port of export of super nano-filtration membrane filter 11 pumps into super nanofiltration liquid pigment temporary storage tank 13 by centrifugal pump 2；
It is temporary that the liquid at the feed liquid membrane concentrated solution outlet end of super nano-filtration membrane filter 11 pumps into super nanofiltration liquid albumen by underflow membrane pump 6
Deposit tank 12.Valve is passed through in the bottom of super nanofiltration liquid albumen temporary storage tank 12 and pipeline is connected with super nanofiltration liquid protein concentration equipment 14.
Super nanofiltration is filtered: the membrane aperture of super nanofiltration separation equipment is 10kda, and pressure is 8bar, and temperature is 36 DEG C.
(6) super nanofiltration liquid protein concentration equipment and protein concentration are reclaimed with ethanol
Using super nanofiltration liquid protein concentration equipment 14, the zein solution in super nanofiltration liquid albumen temporary storage tank 12 is carried out
Concentrate.Open the valve of super nanofiltration liquid albumen temporary storage tank 12 bottom so as in zein liquid to enter excess of imports nanofiltration liquid dense
Contracting equipment 14.The penetrating fluid port of export of super nanofiltration liquid protein concentration equipment 11 pumps into solvent recovery temporary storage tank 16 by centrifugal pump 2
Reclaimed, the liquid at its feed liquid membrane concentrated solution outlet end pumps into protein concentrated solution temporary storage tank 15 by underflow membrane pump 6.
Protein concentration is reclaimed with ethanol: the membrane aperture of super nanofiltration liquid protein concentration equipment is 360da, pressure 8bar, temperature
For 26 DEG C, zein is concentrated, reclaim ethanol simultaneously.
(7) scraper plate vacuum concentration equipment and albumen is concentrated in vacuo
Using scraper plate vacuum concentration equipment 17, the liquid in protein concentrated solution temporary storage tank 15 is carried out concentrated in vacuo.By protein concentration
Protein liquid in liquid temporary storage tank 15 pumps into scraper plate vacuum concentration equipment 17, scraper plate vacuum concentration equipment 17 by underflow membrane pump 6
Top by pipeline connect spiral winding pipe condenser 18, spiral winding pipe condenser reclaim ethanol cooling after through centrifugal pump
2 pump into ethanol recovery temporary storage tank 19 recycles.
Concentrate: temperature is 35 DEG C, pressure is -0.085mpa, the time is 60 min, and being concentrated into alcohol concentration in concentrated solution is
Pump into albumen when 40% and separate out equipment.
(8) it is vacuum dried
Zein wet-milling is dried, baking temperature is 50 DEG C, pressure is 0.2kpa, the time is 120 min, is dried to obtain
5.925kg.
(9) pulverize
By zein powder under the conditions of 30 DEG C, it is ground into the zein powder of 120 mesh.
(10) it is vacuum-packed
By the vacuum packaging of zein powder end, package temperature is room temperature, and vacuum pressure is 0.1pa, pumpdown time 2 s,
70 DEG C of heat-sealing temperature, seals time 5s.
The zein yield of above-mentioned production line and technique productions is 39.5%, and its protein content is 89.25%, warp
Detect its colourity l value 56.25(l value table black and white), a value is red green for 3.28(a value table), b value is blue yellow for 3.65(b value table).
Embodiment 2
The structure of production line is same as Example 1 with arrangement.Concrete controlling of production process is as follows:
(1) solvent allotment: in solvent blend tank, add ethanol and water, it is 90% that ethanol water is deployed into percentage by volume
Solution.
(2) extract: add Zein powder 15kg in countercurrent extraction tank, according still further to Zein powder and ethanol solution with
The solid-to-liquid ratio of 1:8kg/l pumps into ethanol water 120l.It is then turned on the stirring of energy-gathered ultrasonic reactor to extract, airtight in lucifuge
Under the conditions of carry out supersound extraction, Extracting temperature be 50 DEG C, supersonic frequency be 25khz, extraction time be 45min；Stir speed (S.S.) is
100 revs/min.
(3) prefiltration: extract is pumped into the scraper type filter of piece-rate system, filter screen mesh number is 60 mesh, filter pressure
For -0.1mpa, the scraper speed of scraper type filter is 120 revs/min.
(4) ceramic membrane filter: the membrane aperture of ceramic membrane filter is 80um, pressure is 1.5bar, and temperature is 26 DEG C.
(5) super nanofiltration is filtered: the membrane aperture of super nanofiltration separation equipment is 15kda, and pressure is 5bar, and temperature is 38 DEG C.
(6) protein concentration and ethanol reclaim: the membrane aperture of super nanofiltration liquid protein concentration equipment is 360da, and pressure is 5bar,
Temperature is 28 DEG C, carries out ethanol recovery.
(7) concentrating: super nanofiltration filtrate enters scraper plate vacuum concentration equipment and concentrates, thickening temperature is 45 DEG C, pressure is-
0.02mpa, concentration time is 30 min, and being concentrated into alcohol concentration in concentrated solution is to pump into albumen when 40% to separate out equipment, in albumen
Precipitation equipment aqueous precipitation separates out zein wet-milling.The ethanol being concentrated to give is reclaimed simultaneously.
(8) it is dried: baking temperature is 45 DEG C, pressure is 0.5kpa, drying time is 180 min, obtain 5.37kg white
Zein dry powder.
(9) pulverize: by zein powder under the conditions of 35 DEG C, be ground into the zein powder of 150 mesh.
(10) pack: by the vacuum packaging of zein powder end, package temperature is room temperature, and vacuum pressure is 0.5pa, takes out
Vacuum time 10s, 80 DEG C of heat-sealing temperature, seal time 10s.
The zein yield of above-mentioned production line and technique productions is 35.8%, and its protein content is 91.65%, warp
Detect its colourity l value 50.32(l value table black and white), a value is red green for 4.86(a value table), b value is blue yellow for 8.92(b value table).
Embodiment 3
The structure of production line is same as Example 1 with arrangement.Concrete controlling of production process is as follows:
(1) solvent allotment: in solvent blend tank, add ethanol and water, it is 85% that ethanol water is deployed into percentage by volume
Solution.
(2) extract: add Zein powder 15kg in countercurrent extraction tank, according still further to Zein powder and ethanol solution with
The solid-to-liquid ratio of 1:10kg/l pumps into ethanol water 150l.It is then turned on the stirring of energy-gathered ultrasonic reactor to extract, close in lucifuge
Carry out supersound extraction, Extracting temperature is 40 DEG C, supersonic frequency is 15khz, extraction time is 90min under the conditions of closing；Stir speed (S.S.)
For 80 revs/min.
(3) prefiltration: extract is pumped into the scraper type filter of piece-rate system, filter screen mesh number is 100 mesh, filter pressure
For -0.02mpa, the scraper speed of scraper type filter is 100 revs/min.
(4) ceramic membrane filter: the membrane aperture of ceramic membrane filter is 100um, pressure is 1bar, and temperature is 28 DEG C.
(5) super nanofiltration is filtered: the membrane aperture of super nanofiltration separation equipment is 10kda, pressure limit 8bar, and temperature is 36 DEG C.
(6) protein concentration and ethanol reclaim: the membrane aperture of super nanofiltration liquid protein concentration equipment is 360da, and pressure is 8bar,
Temperature is 27 DEG C, carries out ethanol recovery.
(7) concentrating: super nanofiltration filtrate enters scraper plate vacuum concentration equipment and concentrates, thickening temperature is 40 DEG C, pressure is-
0.1mpa, concentration time is 45 min, and being concentrated into alcohol concentration in concentrated solution is to pump into albumen when 40% to separate out equipment, in albumen
Precipitation equipment aqueous precipitation separates out zein wet-milling.The ethanol being concentrated to give is reclaimed simultaneously.
(8) it is dried: baking temperature is 40 DEG C, pressure is 0.8kpa, drying time is 150 min.
(9) pulverize: by zein powder under the conditions of 30 DEG C, be ground into the zein powder of 120 mesh.
(10) pack: by the vacuum packaging of zein powder end, package temperature is room temperature, and vacuum pressure is 0.5pa,
Pumpdown time 10 s, 90 DEG C of heat-sealing temperature, seal time 2s.
The zein yield of above-mentioned production line and technique productions is 36.2%, and its protein content is 90.53%, warp
Detect its colourity l value 48.65(l value table black and white), a value is red green for 2.15(a value table), b value is blue yellow for 5.87(b value table).
In above-mentioned production line the electrical equipment such as all of motor, barrier film underflow pump and centrifugal pump be required to explosion-proof.
Claims (10)
1. a kind of production technology of white zein, be Zein powder is mixed with ethanol water after in lucifuge bar
Carry out dynamic multi-level continuous flow upstream supersound extraction under part；Extract first removes through scraper type filter prefiltration and extracts in solution
Large granular impurity composition, then remove pectin fiber therein through ceramic membrane filter, then make maize yellow through super nanofiltration membrane
Element and small-molecule substance are separated with zein, obtain zein liquid；Zein liquid passes through again
Super nanofiltration membrance concentration simultaneously reclaims alcohol solvent, then through condensation secondary recovery ethanol concentrated in vacuo, the Semen Maydiss of aqueous precipitation precipitation simultaneously
Alcohol soluble protein wet-milling；Zein wet-milling drying, pulverizes, and vacuum packaging obtains white zein product.
2. white zein as claimed in claim 1 production technology it is characterised in that: the volume hundred of ethanol water
Fraction 60% ~ 95%, Zein powder is 1:3 ~ 1:25kg/l with the solid-to-liquid ratio of ethanol water.
3. as claimed in claim 1 a kind of production technology of white zein it is characterised in that: dynamic multi-level is continuously inverse
Stream ultrasonic extraction process is: using dynamic three-level continuous flow upstream supersound extraction, Extracting temperature is 30 DEG C ~ 60 DEG C, and supersonic frequency is
10khz ~ 30khz, extraction time is 15min ~ 120min；Stir speed (S.S.) is 30 ~ 150 revs/min.
4. as claimed in claim 1 a kind of production technology of white zein it is characterised in that: prefiltration technique is:
Scraper type strainer screen mesh number be 60 ~ 120 mesh, filter pressure be -0.02mpa ~ 0.1mpa, stir speed (S.S.) be 70 ~ 120 turns/
Point.
5. as claimed in claim 1 a kind of production technology of white zein it is characterised in that: ceramic membrane filter technique
For: the membrane aperture of ceramic membrane filter is 50 ~ 100um, and pressure is 0 ~ 10bar, and temperature is 20 DEG C ~ 100 DEG C.
6. as claimed in claim 1 a kind of production technology of white zein it is characterised in that: super nanofiltration membrane work
Skill is: super nanofiltration membrane aperture is 10kda ~ 80kda, and pressure is 0 ~ 40bar, and temperature is 20 DEG C ~ 100 DEG C.
7. as claimed in claim 1 a kind of production technology of white zein it is characterised in that: super nanofiltration membrance concentration work
Skill is: super nanofiltration membrane aperture is 360da, pressure limit 0 ~ 40bar, and temperature is 20 DEG C ~ 40 DEG C.
8. as claimed in claim 1 a kind of production technology of white zein it is characterised in that: vacuum concentration process
For: 30 DEG C ~ 45 DEG C of thickening temperature, pressure is -0.02mpa ~ 0.1mpa, and concentration time is 10 min ~ 180 min, is concentrated into dense
In contracting liquid, alcohol concentration is 40%, zein concentrated solution is pumped into albumen and separates out equipment, add water in albumen precipitation equipment
Separate out zein wet-milling；The ethanol simultaneously obtaining concentration condensation is reclaimed.
9. realize as claimed in claim 1 a kind of production line of the production technology of white zein it is characterised in that: bag
Include charging system, extraction system, filtration system, concentration systems, vacuum dryer, pulverizer and vacuum packing machine；
Wherein, charging system includes solvent blend tank (1), and the entrance of solvent blend tank is provided with solvent filter；Solvent is allocated
Tank is connected with extraction system by centrifugal pump；
Extraction system includes countercurrent extraction tank (3), is provided with agitating device (4) in countercurrent extraction tank, installs outside countercurrent extraction tank
There is the ultrasound reactor (5) of UNICOM therewith；Countercurrent extraction tank is connected with filtration system by underflow membrane pump (6)；
Filtration system includes two scraper type filters (7), coarse filtration liquid temporary storage tank (8), purpose ceramic-film filter (9), pottery successively
Membrane filtration liquid temporary storage tank (10), super nano-filtration membrane filter (11), super nanofiltration liquid albumen temporary storage tank (12), super nanofiltration liquid pigment temporary storage tank
(13) seven parts；The liquid outlet of wherein scraper type filter (7) pumps into coarse filtration liquid temporary storage tank (8) by underflow membrane pump (6)；
The liquid of coarse filtration liquid temporary storage tank pumps into purpose ceramic-film filter (9) by underflow membrane pump (6)；The filtrate of purpose ceramic-film filter (9)
Ceramic membrane filtrate temporary storage tank (10) is pumped into by high pressure centrifugal pump；Valve and pipe are passed through in the bottom of ceramic membrane filtrate temporary storage tank (10)
Line is connected with the material inlet end of super nano-filtration membrane filter (11)；The penetrating fluid port of export solution of super nano-filtration membrane filter (11) leads to
Cross high pressure centrifugal pump and pump into super nanofiltration liquid pigment temporary storage tank (13), the feed liquid membrane concentrated solution outlet end of super nano-filtration membrane filter is molten
Liquid pumps into super nanofiltration liquid albumen temporary storage tank (12) by underflow membrane pump (6)；The bottom of super nanofiltration liquid albumen temporary storage tank (12) leads to
Cross valve and pipeline to be connected with super nanofiltration liquid protein concentration equipment (14)；
Concentration systems include super nanofiltration liquid protein concentration equipment (14), protein concentrated solution temporary storage tank (15), solvent recovery temporary storage tank
(16), scraper plate vacuum concentration equipment (17), spiral winding pipe condenser (18), ethanol recovery temporary storage tank (19) and albumen separate out and set
Standby (20) seven parts；The penetrating fluid port of export solution of wherein super nanofiltration liquid protein concentration equipment (14) passes through centrifugal pump (2) pump
Enter solvent recovery temporary storage tank (16), its feed liquid membrane concentrated solution outlet end solution pumps into protein concentration by underflow membrane pump (6)
Liquid temporary storage tank (15)；It is concentrated in vacuo that protein liquid in protein concentrated solution temporary storage tank (15) pumps into scraper plate by underflow membrane pump (6)
Equipment (17)；The bottom of scraper plate vacuum concentration equipment (17) connects albumen by underflow membrane pump (6) and separates out equipment (20), its top
Portion connects spiral winding pipe condenser (18) by pipeline, and the ethanol that spiral winding pipe condenser reclaims is through high pressure centrifugal pump (2)
Pump into ethanol and reclaim temporary storage tank (19).
10. as claimed in claim 9 a kind of production line producing white zein technique it is characterised in that: extract system
The countercurrent extraction tank of system is three-level continuous countercurrent extracting tank, and passes through underflow membrane pump UNICOM, finally between every stage countercurrent extraction pot
One-level countercurrent extraction tank is connected with filtration system by underflow membrane pump.
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| CN109836483A (en) * | 2017-11-28 | 2019-06-04 | 中国石油天然气股份有限公司 | The process units and production method of zeins |
| CN114488937A (en) * | 2022-01-29 | 2022-05-13 | 山东凯斯达机械制造有限公司 | Alcohol extraction protein production control system |
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