CN106342901A - Preparation method of rooting agent for flowering plum - Google Patents

Preparation method of rooting agent for flowering plum Download PDF

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Publication number
CN106342901A
CN106342901A CN201610689095.1A CN201610689095A CN106342901A CN 106342901 A CN106342901 A CN 106342901A CN 201610689095 A CN201610689095 A CN 201610689095A CN 106342901 A CN106342901 A CN 106342901A
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preparation
flowering plum
cellulose
growing agent
enzymolysis
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CN201610689095.1A
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孙国胜
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Jiangsu Oasis Horticulture Co Ltd
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Jiangsu Oasis Horticulture Co Ltd
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Priority to CN201610689095.1A priority Critical patent/CN106342901A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/03—Algae

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides a preparation method of rooting agent for flowering plum. The preparation method includes steps of taking kelp, cleaning and drying the kelp; adding water, cellulose and protease in the kelp; mixing, heating and performing enzymolysis andenzyme deactivation; then adding potassium sulphate and sodium bicarbonate in the enzymatic hydrolysate; heating and preserving temperature; cooling, and condensing material liquid; adjusting pH value of concentrated liquor to 6.0-7.0; at last, adding gelatin, lecithin, wood fiber, cellulose, nicotinamide, amino acid, chitosan, and nicotinamide; dispersing and obtaining the rooting agent. The rooting agent can obviously improve the mean rooting number and cutting survival number of cutting slips of the flowering plum.

Description

A kind of preparation method of flowering plum root-growing agent
Technical field
The invention belongs to field of plant growing technology is and in particular to a kind of preparation method of flowering plum root-growing agent.
Background technology
Flowering plum (prunus triloba) belongs to machaka for Rosaceae Fructus Persicae, is thick with leaves, and spends numerous color gorgeous, and environment is fitted Stress is very strong, is the important sight flowering shrub in north of China spring gardens, and its propagation method mainly adopts plant division and grafting 2 at present The mode of kind.But the survival rate of flowering plum cuttings cuttage is not generally high, taking root, slow and rooting rate is not high, is to hinder flowering plum large area One of principal element of expanding propagation.
At present, the widely used hormone promoting plant establishment and growth regulator are a lot of both at home and abroad, mainly have indole second Sour (iaa), indolebutyric acid (iba), naphthalene acetic acid (naa), gibberellins (gibberellin), the basic element of cell division (gytokinins), Abscisic acid (aba) and abt series root-inducing powder etc..These phytohormone have following weak point: (1) poorly water-soluble.As indole Acetic acid, indolebutyric acid, naphthalene acetic acid, most of gibberellins, abt root-inducing powder etc. are insoluble in water or are slightly soluble in water.During use Need first with dilute with water again after ethanol dissolving, or be allowed to into salt with alkali and be dissolved in water.This not only adds use cost, and Preparation steps are made to increase.(2) natural content low it is difficult to be separated to enough application quantity.Such as 3 tons Brassica oleracea L. var. botrytis L. blades only extract Go out 3 milligrams of heteroauxings, content is part per billion.Per kilogram Semen sojae atricolor contains 14 milligrams of abscisic acid, and content is 14/1000000ths. The content of the zeatin of one of the basic element of cell division is about 1/100000000th.
Content of the invention
The technical problem solving: the purpose of the present invention is to overcome the deficiencies in the prior art and provide a kind of flowering plum with taking root The preparation method of agent, the number of averagely taking root that gained root-growing agent is remarkably improved flowering plum cutting survives number with cutting.
Technical scheme: a kind of preparation method of flowering plum root-growing agent, comprise the following steps:
Step 1, takes Thallus Laminariae (Thallus Eckloniae), cleans, dries, and in parts by weight, adds water 10-12 part, cellulase in 1 part of Thallus Laminariae (Thallus Eckloniae) 0.05-0.1 part and proteinase-10 .05-0.1 part, mixing, it is heated to 40-50 DEG C of enzymolysis 2-4h, enzyme denaturing, obtain enzymolysis solution；
Step 2, in parts by weight, adds potassium sulfate 0.05-0.1 part, sodium bicarbonate 0.01-0.05 part in enzymolysis solution, It is heated to 80-90 DEG C of insulation 3-5h, let cool, feed liquid is concentrated, obtain concentrated solution；
Step 3, adjusts concentrated solution ph to 6.0-7.0, adds gelatin 0.01-0.05 part in parts by weight, lecithin 0.02-0.04 part, wood fibre 0.02-0.05 part, cellulose 0.01-0.03 part, nicotiamide 0.01-0.05 part, aminoacid 0.01-0.05 part, shitosan 0.01-0.05 part, nicotiamide 0.02-0.05 part, dispersion, obtain final product.
Further, in step 1, enzymolysis is in co2Carry out in atmosphere.
Further, in step 2, feed liquid being concentrated into volume-diminished is 15-20%.
Further, in step 3, dispersion condition is 1000-1200rpm, 10-15min.
Further, described cellulose is carboxymethyl cellulose or hydroxypropyl cellulose.
Further, described aminoacid is one of leucine, tryptophan, aspartic acid, arginine or cysteine.
Beneficial effect: the number of averagely taking root that the root-growing agent of the present invention is remarkably improved flowering plum cutting survives number with cutting.
Specific embodiment
Embodiment 1
A kind of preparation method of flowering plum root-growing agent, comprises the following steps:
Step 1, takes Thallus Laminariae (Thallus Eckloniae), cleans, dries, and in parts by weight, adds 10 parts of water, cellulase 0.05 in 1 part of Thallus Laminariae (Thallus Eckloniae) Part and proteinase-10 .05 part, mixing, it is heated to 40 DEG C of enzymolysis 4h, enzyme denaturing, obtain enzymolysis solution；
Step 2, in parts by weight, adds 0.05 part of potassium sulfate, 0.01 part of sodium bicarbonate in enzymolysis solution, is heated to 80 DEG C Insulation 5h, lets cool, feed liquid is concentrated, obtain concentrated solution；
Step 3, adjusts concentrated solution ph to 6.0-7.0, add 0.01 part of gelatin in parts by weight, 0.02 part of lecithin, 0.02 part of wood fibre, 0.01 part of cellulose, 0.01 part of nicotiamide, 0.01 part of aminoacid, 0.01 part of shitosan, nicotiamide 0.02 Part, dispersion, obtain final product.
Wherein, in step 1, enzymolysis is in co2Carry out in atmosphere；In step 2, feed liquid being concentrated into volume-diminished is 15%； In step 3, dispersion condition is 1000rpm, 15min；Described cellulose is carboxymethyl cellulose；Described aminoacid is leucine.
Embodiment 2
A kind of preparation method of flowering plum root-growing agent, comprises the following steps:
Step 1, takes Thallus Laminariae (Thallus Eckloniae), cleans, dries, and in parts by weight, adds 10 parts of water, cellulase 0.07 in 1 part of Thallus Laminariae (Thallus Eckloniae) Part and proteinase-10 .06 part, mixing, it is heated to 45 DEG C of enzymolysis 3h, enzyme denaturing, obtain enzymolysis solution；
Step 2, in parts by weight, adds 0.07 part of potassium sulfate, 0.04 part of sodium bicarbonate in enzymolysis solution, is heated to 85 DEG C Insulation 4h, lets cool, feed liquid is concentrated, obtain concentrated solution；
Step 3, adjusts concentrated solution ph to 6.0-7.0, add 0.03 part of gelatin in parts by weight, 0.02 part of lecithin, 0.04 part of wood fibre, 0.02 part of cellulose, 0.04 part of nicotiamide, 0.03 part of aminoacid, 0.03 part of shitosan, nicotiamide 0.04 Part, dispersion, obtain final product.
Wherein, in step 1, enzymolysis is in co2Carry out in atmosphere；In step 2, feed liquid being concentrated into volume-diminished is 18%； In step 3, dispersion condition is 1100rpm, 15min；Described cellulose is hydroxypropyl cellulose；Described aminoacid is tryptophan.
Embodiment 3
A kind of preparation method of flowering plum root-growing agent, comprises the following steps:
Step 1, takes Thallus Laminariae (Thallus Eckloniae), cleans, dries, and in parts by weight, adds 11 parts of water, cellulase 0.06 in 1 part of Thallus Laminariae (Thallus Eckloniae) Part and proteinase-10 .08 part, mixing, it is heated to 40 DEG C of enzymolysis 4h, enzyme denaturing, obtain enzymolysis solution；
Step 2, in parts by weight, adds 0.08 part of potassium sulfate, 0.02 part of sodium bicarbonate in enzymolysis solution, is heated to 80 DEG C Insulation 5h, lets cool, feed liquid is concentrated, obtain concentrated solution；
Step 3, adjusts concentrated solution ph to 6.0-7.0, add 0.04 part of gelatin in parts by weight, 0.03 part of lecithin, 0.03 part of wood fibre, 0.02 part of cellulose, 0.04 part of nicotiamide, 0.03 part of aminoacid, 0.02 part of shitosan, nicotiamide 0.04 Part, dispersion, obtain final product.
Wherein, in step 1, enzymolysis is in co2Carry out in atmosphere；In step 2, feed liquid being concentrated into volume-diminished is 15%； In step 3, dispersion condition is 1000rpm, 15min；Described cellulose is carboxymethyl cellulose；Described aminoacid is arginine.
Embodiment 4
A kind of preparation method of flowering plum root-growing agent, comprises the following steps:
Step 1, takes Thallus Laminariae (Thallus Eckloniae), cleans, dries, and in parts by weight, adds 12 parts of water, 0.1 part of cellulase in 1 part of Thallus Laminariae (Thallus Eckloniae) With proteinase-10 .1 part, mix, be heated to 50 DEG C of enzymolysis 2h, enzyme denaturing, obtain enzymolysis solution；
Step 2, in parts by weight, adds 0.1 part of potassium sulfate, 0.05 part of sodium bicarbonate in enzymolysis solution, is heated to 90 DEG C Insulation 3h, lets cool, feed liquid is concentrated, obtain concentrated solution；
Step 3, adjusts concentrated solution ph to 6.0-7.0, add 0.05 part of gelatin in parts by weight, 0.04 part of lecithin, 0.05 part of wood fibre, 0.03 part of cellulose, 0.05 part of nicotiamide, 0.05 part of aminoacid, 0.05 part of shitosan, nicotiamide 0.05 Part, dispersion, obtain final product.
Wherein, in step 1, enzymolysis is in co2Carry out in atmosphere；In step 2, feed liquid being concentrated into volume-diminished is 20%； In step 3, dispersion condition is 1200rpm, 10min；Described cellulose is carboxymethyl cellulose；Described aminoacid is cysteine.
Collection life in 1,2 years, the full top stalwartness flowering plum branch of terminal bud is cutting, takes long 6-8cm.With 0.5% height Potassium manganate solution is sterilized, after put it in embodiment 1 to 4 gained and process about 24h, and entirely insert in and sterilized in loam, observe Its situation of taking root.Compared with 90 μ g/ml naphthalene acetic acids, every group processes 100, make 2 repetitions.Test temperature is 15-25 DEG C, fits Preferably humidity is 70-80%.Take out after 3 months and observe its situation of taking root, result is as follows:
Comparison Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4
Take root number/bar 7 10 9 11 10
The longest root length/cm 0.3 0.5 0.6 0.5 0.6
Average root length/cm 0.23 0.45 0.51 0.55 0.49
Total root length/cm 1.8 2.7 3.2 3.5 2.9
Cuttage survival rate/% 53 82 84 87 83
As seen from the above table, the root-growing agent of the present invention be remarkably improved flowering plum cutting averagely take root number and cutting survive Number.

Claims (6)

1. a kind of preparation method of flowering plum root-growing agent it is characterised in that: comprise the following steps:
Step 1, takes Thallus Laminariae (Thallus Eckloniae), cleans, dries, and in parts by weight, adds water 10-12 part, cellulase 0.05- in 1 part of Thallus Laminariae (Thallus Eckloniae) 0.1 part and proteinase-10 .05-0.1 part, mixing, it is heated to 40-50 DEG C of enzymolysis 2-4h, enzyme denaturing, obtain enzymolysis solution；
Step 2, in parts by weight, adds potassium sulfate 0.05-0.1 part, sodium bicarbonate 0.01-0.05 part, heating in enzymolysis solution To 80-90 DEG C of insulation 3-5h, let cool, feed liquid is concentrated, obtain concentrated solution；
Step 3, adjusts concentrated solution ph to 6.0-7.0, adds gelatin 0.01-0.05 part in parts by weight, lecithin 0.02- 0.04 part, wood fibre 0.02-0.05 part, cellulose 0.01-0.03 part, nicotiamide 0.01-0.05 part, aminoacid 0.01- 0.05 part, shitosan 0.01-0.05 part, nicotiamide 0.02-0.05 part, dispersion, obtain final product.
2. flowering plum root-growing agent according to claim 1 preparation method it is characterised in that: in step 1 enzymolysis be co2Carry out in atmosphere.
3. flowering plum root-growing agent according to claim 1 preparation method it is characterised in that: in step 2, feed liquid is dense Being reduced to volume-diminished is 15-20%.
4. flowering plum root-growing agent according to claim 1 preparation method it is characterised in that: dispersion condition in step 3 For 1000-1200rpm, 10-15min.
5. flowering plum root-growing agent according to claim 1 preparation method it is characterised in that: described cellulose be carboxylic first Base cellulose or hydroxypropyl cellulose.
6. flowering plum root-growing agent according to claim 1 preparation method it is characterised in that: described aminoacid be bright ammonia One of acid, tryptophan, aspartic acid, arginine or cysteine.
CN201610689095.1A 2016-08-18 2016-08-18 Preparation method of rooting agent for flowering plum Pending CN106342901A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109251085A (en) * 2018-08-31 2019-01-22 镇江鑫源达园艺科技有限公司 A kind of preparation method of shallot taking root liquid
CN117918140A (en) * 2024-03-25 2024-04-26 海南红芯椰子研究开发有限公司 Cutting rooting propagation method for oleander

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103183567A (en) * 2013-04-01 2013-07-03 张元宝 Liquid bacterial agent for rooting
CN103694035A (en) * 2013-12-02 2014-04-02 沃太能源南通有限公司 Preparation method of sustained released type nutrient for soilless culture of cucumbers
CN103694034A (en) * 2013-12-02 2014-04-02 沃太能源南通有限公司 Preparation method of compound fertilizer dedicated to ginkgo trees by using blue-green algae as base material
CN104151080A (en) * 2014-08-26 2014-11-19 威海市世代海洋生物科技有限公司 Preparation method of plant nutrient solution

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103183567A (en) * 2013-04-01 2013-07-03 张元宝 Liquid bacterial agent for rooting
CN103694035A (en) * 2013-12-02 2014-04-02 沃太能源南通有限公司 Preparation method of sustained released type nutrient for soilless culture of cucumbers
CN103694034A (en) * 2013-12-02 2014-04-02 沃太能源南通有限公司 Preparation method of compound fertilizer dedicated to ginkgo trees by using blue-green algae as base material
CN104151080A (en) * 2014-08-26 2014-11-19 威海市世代海洋生物科技有限公司 Preparation method of plant nutrient solution

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109251085A (en) * 2018-08-31 2019-01-22 镇江鑫源达园艺科技有限公司 A kind of preparation method of shallot taking root liquid
CN117918140A (en) * 2024-03-25 2024-04-26 海南红芯椰子研究开发有限公司 Cutting rooting propagation method for oleander

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Application publication date: 20170125