CN106342687A - A rapid propagation method of Dendrobium candidum - Google Patents
A rapid propagation method of Dendrobium candidum Download PDFInfo
- Publication number
- CN106342687A CN106342687A CN201610760000.0A CN201610760000A CN106342687A CN 106342687 A CN106342687 A CN 106342687A CN 201610760000 A CN201610760000 A CN 201610760000A CN 106342687 A CN106342687 A CN 106342687A
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- China
- Prior art keywords
- dendrobium candidum
- medium
- herba dendrobii
- culture
- quickly breeding
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention is applicable to the field of plant culture technology improvement, and provides a method for rapid propagation of Dendrobium candidum, which comprises the following steps:A, configuration of effective medium: The B5 basic medium is used for culturing, 0.6 - 0.9mg / L NAA, 0.3 - 0.6mg / L6 BA and 210 - 300g / L potato juice are added and sterilized at 135 oC for 18min; Transferring the original bulb to the configured medium;C, culture conditions: the light time is 9-11 hours a day, the light intensity is 2500lx, and the temperature is 27 oC.Using a unique liquid culture formula for the quick and stable proliferation of protocorms, providing a short cycle, high rate of reproduction, low cost of seedling methods for the large-scale cultivation of Dendrobium candidum.
Description
Technical field
The invention belongs to biotechnology improvement areas, more particularly, to a kind of method for quickly breeding of Herba Dendrobii.
Background technology
Herba Dendrobii is listed in " first of Chinese nine big Herba mesonae chinensis ", since the Tang and Song Dynasty, has been listed in royal tribute always.Li Shizhen (1518-1593 A.D.)
Compendium of Material Medica is evaluated Herba Dendrobii " reinforcing YIN-essence benefit essence, thick the intestines and stomach, never sufficient in benefit, flat stomach gas, long muscle, Fructus Alpiniae Oxyphyllae remove frightened,
Make light of one's life by commiting suicide macrobiosis "；Among the people it is called " help Herba mesonae chinensis ".Additionally, the stem of Herba Dendrobii can clearing away heat and promoting production of body fluid, anti-inflammatory analgetic, clear and rich larynx
Throat, has good curative effect to treatment hoarse voice.
Herba Dendrobii is typically grown between height above sea level 100-3000 rice height, often grows nonparasitically upon another plant in tree above or on rock, happiness temperature
Warm, moistening and half shade environment, can not resist cold.18～30 DEG C of thermophilic of growth, trophophase is with 16～21 DEG C of more particularly suitable, rest periods
16～18 DEG C, evening temperature is 10～13 DEG C, and the temperature difference is maintained at 10～15 DEG C most preferably.Day temperature is more than 30 DEG C to stone
Less, winter temperature is not less than 10 DEG C to dry measure used in former times growth effect.Seedling is easily endured cold below 10 DEG C, and the environmental condition of growth is required
Harsh.The natural propagation ability of dendrobium officinale is low, poor growth.
The expanding propagation of Herba Dendrobii protocorm, is the main path of Herba Dendrobii breeding, protocorm is that seed tissue is cultivated
Come.Because of Seed storage limited time, do not reach the purpose of Immortalization, so, can only by the preservation of protocorm and expanding propagation Lai
Fast-propagation Herba Dendrobii.Solid medium is easy to be divided into protocorms, and then develops seedling, and do not reach protocorm
The preservation of stem and the purpose of Fast-propagation.
Content of the invention
It is an object of the invention to provide a kind of method for quickly breeding of Herba Dendrobii it is intended to solve problem.
The present invention is achieved in that a kind of method for quickly breeding of Herba Dendrobii, described method for quickly breeding include with
Lower step:
A, the effective culture medium of configuration: culture medium adopts b5 minimal medium, adds 0.6-0.9mg/lnaa, 0.3-0.6mg/l6-
Ba and 210-300g/l murphy juice, makes in 135 DEG C of sterilizing 18min；
B, protocorm is transferred in the culture medium having configured；
C, condition of culture: light application time is daily 9-11 hour, light intensity is 2500lx, and temperature is 27 DEG C.
The further technical scheme of the present invention is: in b5 minimal medium in described step a add 0.8mg/lnaa,
0.5mg/l6-ba and 280g/l murphy juice, makes in 135 DEG C of sterilizing 18min.
The further technical scheme of the present invention is: the light application time in described step c is daily 10 hours.
The invention has the beneficial effects as follows: using unique liquid culture based formulas, make Protocorm Multiplication fast and stable, be ferrum
The implant mass of skin Herba Dendrobii provides a kind of cycle is short, breeding potential method for culturing seedlings high, with low cost.Achieve Herba Dendrobii
Safe and efficient cultivation, not only improve the protection of the dendrobium officinale resource to disappearance in imminent danger, promote with Herba Dendrobii again
The development of the Chinese medicine enterprise of raw material, can bring good ecological effect, economic benefits and social effect.
Specific embodiment
The method for quickly breeding of the Herba Dendrobii that the present invention provides, details are as follows:
Step a, configures effective culture medium: culture medium adopts b5 minimal medium, adds 0.6-0.9mg/lnaa, 0.3-
0.6mg/l6-ba and 210-300g/l murphy juice, makes in 135 DEG C of sterilizing 18min；Wherein, add 0.8mg/lnaa,
0.5mg/l6-ba and 280g/l murphy juice, the culture medium made in 135 DEG C of sterilizing 18min is preferred plan.
Step b, protocorm is transferred in the culture medium having configured.
Step c, condition of culture: light application time is daily 9-11 hour, light intensity is 2500lx, and temperature is 27 DEG C.Wherein,
Light application time is to be within daily 10 hours preferred plan.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (3)
1. a kind of method for quickly breeding of Herba Dendrobii is it is characterised in that described method for quickly breeding comprises the following steps:
A, the effective culture medium of configuration: culture medium adopts b5 minimal medium, adds 0.6-0.9mg/lnaa, 0.3-0.6mg/l6-
Ba and 210-300g/l murphy juice, makes in 135 DEG C of sterilizing 18min；
B, protocorm is transferred in the culture medium having configured；
C, condition of culture: light application time is daily 9-11 hour, light intensity is 2500lx, and temperature is 27 DEG C.
2. method for quickly breeding according to claim 1 is it is characterised in that add in b5 minimal medium in described step a
Enter 0.8mg/lnaa, 0.5mg/l6-ba and 280g/l murphy juice, make in 135 DEG C of sterilizing 18min.
3. method for quickly breeding according to claim 2 is it is characterised in that the light application time in described step c is daily
10 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610760000.0A CN106342687A (en) | 2016-08-30 | 2016-08-30 | A rapid propagation method of Dendrobium candidum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610760000.0A CN106342687A (en) | 2016-08-30 | 2016-08-30 | A rapid propagation method of Dendrobium candidum |
Publications (1)
| Publication Number | Publication Date |
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| CN106342687A true CN106342687A (en) | 2017-01-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610760000.0A Pending CN106342687A (en) | 2016-08-30 | 2016-08-30 | A rapid propagation method of Dendrobium candidum |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107466864A (en) * | 2017-09-30 | 2017-12-15 | 中国科学院成都生物研究所 | A kind of method of dendrobium candidum vegetative propagation sprouting and rooting |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919123A (en) * | 2012-10-30 | 2013-02-13 | 大连斯扶特农业科技有限公司 | Rapid propagating method of dendrobium candidum |
| CN102948368A (en) * | 2012-11-13 | 2013-03-06 | 安徽新津铁皮石斛开发有限公司 | Tissue culture method of dendrobium candidum |
| CN103947549A (en) * | 2014-04-17 | 2014-07-30 | 厦门涌泉科技有限公司 | Rapid propagation method of dendrobium officinale |
| CN104509440A (en) * | 2014-12-22 | 2015-04-15 | 广西南宁桂知科技有限公司 | Culture medium for culturing dendrobium officinale tissue cultured seedling and method for preparing culture medium |
-
2016
- 2016-08-30 CN CN201610760000.0A patent/CN106342687A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919123A (en) * | 2012-10-30 | 2013-02-13 | 大连斯扶特农业科技有限公司 | Rapid propagating method of dendrobium candidum |
| CN102948368A (en) * | 2012-11-13 | 2013-03-06 | 安徽新津铁皮石斛开发有限公司 | Tissue culture method of dendrobium candidum |
| CN103947549A (en) * | 2014-04-17 | 2014-07-30 | 厦门涌泉科技有限公司 | Rapid propagation method of dendrobium officinale |
| CN104509440A (en) * | 2014-12-22 | 2015-04-15 | 广西南宁桂知科技有限公司 | Culture medium for culturing dendrobium officinale tissue cultured seedling and method for preparing culture medium |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107466864A (en) * | 2017-09-30 | 2017-12-15 | 中国科学院成都生物研究所 | A kind of method of dendrobium candidum vegetative propagation sprouting and rooting |
| CN107466864B (en) * | 2017-09-30 | 2019-10-08 | 中国科学院成都生物研究所 | A kind of method of dendrobium candidum vegetative propagation sprouting and rooting |
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Application publication date: 20170125 |