CN106167297A - A kind of method and application preparing vegetable active hydrogen water based on biological engineering - Google Patents
A kind of method and application preparing vegetable active hydrogen water based on biological engineering Download PDFInfo
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Abstract
The invention goes out the method that one utilizes biological engineering " quasi-anaerobism, micro-fermentation " technique to produce plant hydrogen rich water.By activated plant active matter stock solution, produce reactive hydrogen, be dissolved in water, make plant hydrogen rich water.The characteristic parameter of plant hydrogen rich water includes: water temperature, activate duration, pH value, containing hydrogen concentration, oxidation-reduction potential." quasi-anaerobism, micro-fermentation " technique of the present invention, can be widely applied to the reactive hydrogen in activated plant body, forms the plant hydrogen rich water that performance is different.Every kind of plant all have can measure, can distinguish, comparable antioxidation quantitative characteristics, make the present invention likely move towards modernization for Chinese medicine, scientific, internationalization provides a kind of potential quantitative analysis tools.Utilize the technology of the present invention, can produce various types of plant hydrogen-rich carrier: hydrogen-rich fruit and vegerable, hydrogen-rich Chinese herbal medicine, the health care of hydrogen-rich grain class, health preserving, cosmetics.
Description
Technical field
The method that the invention belongs to prepare hydrogen rich water, a kind of side preparing vegetable active hydrogen water based on biological engineering
Method and application.
Background technology
Germany's promise recent years youngster steps on water and the legend story of France's Lu Erde holy water, initiation hydrogeneous of Nao grottoes mystery
Spring water has the Science Explorations upsurge of disease cure function and grinding of Japanese Scientists, the hydrogen molecule medical science of enterpriser's promotion and hydrogen rich water
System, promotes carrying out of biomedical all over the world and life and health expert's R&D work.Focus of concern is that universe accounts for
The 90% of total elemental composition, accounts for the protium of more than 80% in organism.The research of hydrogen biological effect has been found that hydrogen has
Selective neutralization hydroxy radical and the characteristic of peroxynitrite, be the active substance treating all kinds of oxidative damages, be
Excellent antioxidant.Therefore, hydrogen, at treatment disease, slow down aging, is promoted healthy, biosystem management aspects tool
There is the most tempting prospect.The most various artificial holy water arise at the historic moment, for carrying out scientific research further and providing people's health preserving
Health care provides multiple hydrogenation means.The birth of hydrogen molecule medical science and development also provide the unlimited broad hydrogen that relates to for enterpriser and produce
The market of industry and new opportunity, new challenge.At present, main have to hydrogen means or product following several: directly breathe hydrogen
(suction hydrogen)；Inject hydrogeneous normal saline (note hydrogen) and drink hydrogen rich water (drinking hydrogen).The most prevailing is to drink hydrogen rich water, compares peace
Entirely, convenient and effective.On market today, the hydrogen rich water of supply or the equipment of generation hydrogen rich water have three kinds: electrolytic hydrogen production water, metal
Magnesium and water react the hydrogen water produced and physics hydrogen manufacturing water (utilizing micro-nano bubble solubilising).It addition, physician finds, in human body
Containing endogenous hydrogen.The antibacterial of big enteral can produce hydrogen.Therefore, also occur in that another kind is to hydrogen mode: oral cellulose
Or modified starch is to induce the raw more hydrogen of big enteral Production by Bacteria, the purpose of reach to cure the disease (such as protecting liver sausage).Additionally, also
The oral hydrogen-rich capsule that useful veterinary antibiotics or other food are made, eats in human body, produces endogenous hydrogen.Make a general survey of presently commercially available
Hydrogen rich water, the overwhelming majority is in pure water, injects with physico-chemical method or generates hydrogen online, makes higher hydrogeneous of concentration
Water.Individual product, is with the drinking water having met water quality standard generally acknowledged, makes hydrogeneous good water.Such as: natural mineral spring hydrogen rich water.
Also have a patent " hydrogen-rich health beverage and preparation method thereof " (CN105105256A) unusual, be hydrogen is dissolved in water-soluble
In the nutrient liquid of property plant extract, make the composite beverage also comprising the nutrient useful to human body in addition to hydrogen.But
It is that the hydrogen in current so-called hydrogen rich water or hydrogen-rich beverage is all to be added to pure by hydrogen prepared by outside without any exception
In water or good water or nutritional solution, it is that the hydrogen prepared with chemistry or physico-chemical method, is not naturally-produced hydrogen by manually,
It is less endogenous hydrogen.Intuitively, the judgement of common-sense, made hydrogen rich water, hydrogeneous less than natural, naturally occurring
Water.Particularly potential curing the disease or aspect is drunk in health care as a kind of.Certainly, for medical research, in order to reduce it
The interference of his factor, using pure water or normal saline Hydrogenation is necessary, useful for hydrogen rich water.
It is known that the primary efficacy of reactive hydrogen is by eliminating " bad " free radical or poisonous free radical selectively
Prevent, protect mankind's human body and cell to damage from over oxidation.The biomedical achievement in research of hydrogen molecule is pointed out: active oxygen
The toxic radicals (hydroxyl radical free radical and peroxynitrite) produced is the main cause given rise to diseases, therefore,
Reactive hydrogen is a kind of maximally effective, antioxidant of having no side effect protecting health.The evolution of biosphere have passed through millions of
Year, from simple Cellular evolution to plant, animals and humans.Plant is in ground fixed growth, it is impossible to mobile, constantly through wind-engaging
The test of the harshest natural environment such as blowing rain to beat, the sun tans by the sun, ultraviolet irradiation.According to theory of evolution, survival of the fittest, discomfort person
The rule eliminated, leaves all kinds of plants grown the most on earth at present: trees, weeds, veterinary antibiotics, corn.They be with
The optimal winner that it bucket resists with ground.The animals or humans that can walk about, due to (he) can avoid by walking about escape
The injury of severe large natural environment, so congenitally not having the strongest oxidation resistance of plant.With the hydrogen unit in human body
As element, hydrogen is also plant endogenous hydrogen intrinsic, more more than animals and humans, more effective in plant, antioxidant.Day
The author of this doctor of medicine Mr. Lin Xiuguang-" water of life-hydrogen rich water toxin expelling " book, (Chinese translation the 44th in his works
Page) point out: " plant is all polyphenoils originally, and the big China fir of centuries in past or the more than one thousand years age of tree was once enjoyed fleeting fame.But either
Trees or flowers and plants are all the plants that will not move, they always accept dumbly ultraviolet irradiate brought fierce
The baptism of active oxygen.Even so, the most how to experience hundreds of more than one thousand years and come back to life？... their own has place
The ability of reason active oxygen ... regardless of plant, they are internal itself is owned by polyphenoils, in other words plant inherently
It it is polyphenoils.But ", in book or among publication disclosed in other the most further research plant be polyphenoils this
Proposition.More do not exclusively carry out research, verify with great many of experiments.So far, also rest in conjecture aspect.It is true that explore
The scientist of new forms of energy carried out numerous studies and experiment from the angle of the energy, it was demonstrated that certain plants can be by certain
Method, produces hydrogen, becomes one of new clean energy resource.The research of bio-hydrogen production technology is achieved with certain progress.Patent " utilizes
The preparation method (CN1176216C) of the fermented liquid for biologically producing hydrogen of straw " it is exactly a concrete application case.Demonstrate
There is hydrogen inside straw, hydrogen can be extracted as a kind of clear energy sources by fermentation process.The master of the present invention
Syllabus attempt to look for a kind of method exactly, plant can be disclosed generally there is non-oxidizability, this method is applicable to all of planting
Thing, is used for verifying " plant is all polyphenoils " this proposition.Meanwhile, this method can be used for preparing biological hydrogen rich water, a kind of
New to hydrogen mode.
Summary of the invention
Currently, with respect to utilizing the mechanism of biohydrogen, more existing primary explanation.Biohydrogen includes photosynthetic bacteria
Produce the product hydrogen means that hydrogen, bacterial anaerobic fermentation product hydrogen and first two method are used in mixed way.Immediate with the present invention is that anaerobism is thin
Bacterium fermentation and hydrogen production.Zymogenous bacteria produces the theory of hydrogen and can be used to the reference as the present invention, develops into a kind of new method.Become
Activate the means of the hydrogeneous inherent character of all plants.Existing open file elaborates the mechanism of fermentation and hydrogen production: according to formation
Zymogenous bacteria is divided into three major types by the electron donor of hydrogen: the special bacterial groups of anaerobism, amphimicrobian bacterial groups and desulfurization
Vibrio produces hydrogen under the conditions of without sulfur.Having found can be for the antibacterial of fermentation and hydrogen production kind more than 20.Personnel separate in a large number, sieve after deliberation
Find after choosing: substantially only have two kinds of antibacterials in action.That is, some strains of fusobacterium and Enterobacter.Zymogenous bacteria
By multiple Substrate hydrolysis preparing hydrogen under the catalytic action of hydrogenlyase or azotase.In the present invention, we are by Chinese herbal medicine, water
Fruits and vegetables and the aqueous solution of grain or active substance of plant aqueous solution are as substrate, because these plants are all by light
The Organic substance that cooperation can grow with conversion solar.Formic acid, lactic acid, glucose, starch, cellulose, each is comprised in these substrates
Plant the composition such as short-chain fatty acid and sulfide.Zymogenous bacteria under hydrogen catabolic enzyme catalytic action and substrate generation biochemical reaction, produces
Raw hydrogen.Practice finds, if strictly to carry out anaerobic biochemical reaction, it is necessary to filling nitrogen or other inertia in the reactor
Gas, all replaces away the oxygen of the inside.Under these conditions, complete reaction time the longest the longest, good some months,
Even more than 1 year.Inefficiency.But, the purpose that the present invention is to be reached, it is different from exploitation hydrogen energy source.Can not indiscriminately imitate existing
For developing the anaerobic ferment process of hydrogen energy source, it is necessary to improve, found the new technology meeting application claims.
It is emphasized that Activiation method to be invented, it should let nature take its course, genuine, need not artificially add other
Material, it is therefore an objective to can obtain measurand instinct, natural, real attribute.The sky that this natural activation method is obtained
So characteristic, can be used for comparing all plants and differentiating have universality.On the other hand, have specific characteristics, same is special
Property, with different plants, in character and tolerance, it is different, differentiated.Secondly as each plant must have
There is the attribute of many aspects, so when considering the general characteristic what feature describes and measures each monomer with, it is impossible to
Exhaustive, it should to concentrate on crucial points, prominent principal contradiction.The biomedical achievement in research of hydrogen molecule shows: by non-oxidizability
Power or containing reactive hydrogen number characterize the attribute of plant it appear that reasonably select.Catch this point, just caught
At all.The invention solves the problems that two large problems: first, how to activate out by the activation hydrogen of plant；Second, determine which is mainly joined
Number can represent the antioxidant characteristics of described plant.
The present invention proposes a solution: first determine that the liquid by comprising active components of plants is (the most former
Liquid) as object of study.Secondly, find a kind of method, the reactive hydrogen in above-mentioned stock solution can be ejected, be dissolved in the water,
Form plant hydrogen rich water.Finally, in the plant hydrogen rich water prepared by detection, characterize oxidation resistance Chief parameter--
Containing hydrogen concentration and oxidation-reduction potential.The preparation method of described stock solution has two kinds: laboratory method and industrial method.Experiment
Room method-and in drinkable water, put into plant according to a certain percentage, heat, boil 100 degrees Celsius, boil 30 minutes, natural
Cooling, obtains stock solution.Industrial method-first pulverized by plant high speed disintegrating machine, adds a certain proportion of drinkable water.So
After, separate with high speed centrifuge, after precipitation, take supernatant, obtain stock solution.Described Activiation method, is by the quasi-anaerobism of stock solution, micro-
The technique of fermentation is processed, and produces hydrogen, is dissolved in water, generate plant hydrogen rich water in stock solution.Described detection plant hydrogen rich water
Non-oxidizability parameter, refer to measure in hydrogen rich water the concentration C (H of the hydrogen dissolved2) and oxidation-reduction potential ORP.These are two years old
Individual parameter will be measured simultaneously.
The technique the most why using " quasi-anaerobism and micro-fermentation ".Usual employing already mentioned before by plant
Hydrogen can be produced by anaerobic fermentation.This technique will be by the oxygen in stock solution by nitrogen injection all outer rows.Then dark fermentation, warp
Spend two stages, be even more than 1 year up to the several months and could realize producing hydrogen continuously.We are not intended to produce continuously hydrogen, and
It is to generate hydrogen rich water.Target is different, and method is different.Facts have proved that the technique using the present invention is largely effective.As long as one day to several
It time just can complete whole activation process, generates the plant hydrogen rich water the highest containing hydrogen concentration and reduction potential.Why
Use micro-fermentation, be that acidity is the highest, can consume hydrogen due to degree of depth fermentation or excessive fermentation, the hydrogen making to have ejected dense
Degree gradually lowers.So attenuation degree must be controlled.At micro-fermentation stage, the reactive hydrogen concentration of generation just can reach maximum.
Owing to a large amount of plants are measured anti-oxidation characteristics, the process efficiency of the present invention is the highest, is very suitable for laboratory and gathers big
The needs of data.Meanwhile, also it is beneficial to be prepared expeditiously the large-scale industrial production of plant hydrogen rich water.
Owing to selecting to measure contained reactive hydrogen concentration and contained free electron quantity (OPR in the plant hydrogen rich water being activated
Current potential) characterize the underlying attribute-oxidation resistance of every kind of plant, and concentration and current potential all can be measured, and then, antioxygen
Change ability can quantify, it is achieved biometrics.Oxidation resistance height according to every kind of plant, identifies and distinguishes different plant
Function." label " to certain plant.This method can be used for all of plant, including Chinese herbal medicine, water fruits and vegetables and paddy
Thing.Accordingly, a large database concept comprising all plants can be set up.
Why it is absorbed in detection plant hydrogen rich water feature, goes out to send from hydrogen molecule biomedicine angle and consider a problem.
It is the factor having caught harm biological existence most critical, i.e. the source of ten thousand diseases is that the toxic radicals of activity in vivo oxygen generation is to carefully
Born of the same parents and the infringement of organ, and endogenous activity hydrogen is the jinx of maximally effective removing toxic radicals.Reactive hydrogen, elimination is utilized to have
Evil free radical, reaches people's vivo oxidation reduction optimum balance state, makes the mankind avoid the generation of many chronic diseases, or treatment is sent out
Raw multiple disease, makes human normal life, lets nature take its course, it is to avoid senilism, delaying aging, and life lengthening is healthy, beautiful, pleased
Live soon.Therefore, every kind of Chinese herbal medicine or food, melon and fruit, the feature of grain are identified by oxidation resistance, it should say it is reasonable
, useful.Certainly, it is desirable to test environment, test condition are wanted standardization, unitized.Such as；Standard pressure, standard temperature and
Standard back substrate concentration etc..
The Activiation method of concrete Laboratary type is；
1, get out measurand, it is necessary to without pesticide, pollute without fertilizer residue.If there being slight pollution to can not find again replacement
Thing, should be purged pesticide and fertilizer residue processing in advance.If measurand is root block, thick stem, nut and large-scale wooden
Element, first has to pulverize after drying, to the granular size after pulverizing without being strict with, less than 3m.These fresh and dried plants
Leaf, rhizome, fruit or their powder, using the substrate as stock solution.
2, regulate laboratory temperature to 25 DEG C, atmospheric pressure is 0.1MPa；All measuring instruments are calibrated.Including sky
Flat, timer, ORP tester, pH tester, jigger, thermometer, dissolved hydrogen concentration measurement instrument etc..
3, prepare a marmite, or stainless-steel pan, total measurement (volume) is not less than 600ml.Put into 6 grams of substrates-be equivalent to volume
1% mass percent of 600ml.As being dry material, such as Chinese herbal medicine and Folium Camelliae sinensis etc., quality should halve, i.e. 3 grams, 0.5% mass
Percentage ratio.As the case may be, mass percent rises to 3%.Then drinking water is filled, till filling it up with 600ml.With literary composition
Fire adds hot terrine, boils, and stops after decocting 30 minutes substrates.Here it is for the stock solution activated.
4, then take a glass graduate heat-resisting, that have 500ml graduation mark, 500ml graduation mark distance rim of a cup about 20 is public
Point.The above-mentioned boiled upper strata stock solution prepared is poured in measuring cup, till filling, does not interspaces.Use food fresh keeping while hot
Film stretches tight sealed beaker mouth, stands, natural cooling, make temperature from close to 100 degree be reduced to 25-30 spend time, open rapidly preservative film,
Pour out a part of stock solution, until 500ml graduation mark, reserve the space of about 20 centimeters, be full of fresh, clean, warm and humid sky
Gas.By this step process, it is known that: the space vessel wall of stock solution and the Bei Zhong the superiors about 20% all have passed through high temperature sterilization sterilization.
Air in space contains a small amount of oxygen and natural probiotic bacteria.It is again sealed off, is ready for " quasi-anaerobism, micro-fermentation ", activate
Stock solution, produces hydrogen.
5, the beaker filling stock solution of good seal is moved in insulating utensils, thermophore temperature is adjusted to 25-35 DEG C.Connect electricity
Source, starts activation technology program, keeps the temperature of liquid in glass constant.After being incubated 12 hours, start to measure the master of liquid
Want parameter.First sampling 20 milliliters, detects oxidation-reduction potential ORP, if nagative potential, illustrates that activating hydrogen is excited
Out, the measurement of other parameters can be carried out.Otherwise, it is not necessary that carry out the measurement of other parameters.Note carrying out at every sub-sampling
During measurement, sample will first cool to standard state 25 degrees Celsius.If not yet reaching maximum containing hydrogen concentration, continuing insulation and swashing
Live.Repeat this program once every 4～8 hours, detect and record one group of parameter, including: date, time, water temperature, during activation
Length, pH value, containing hydrogen concentration, oxidation-reduction potential (ORP), until the difference of adjacent two secondary data, stop when change is less than 2%.Special
Not measure and to record whole parameters in 24 hour that moment of insulation.During measurement, should by record every time containing hydrogen concentration
Mark with oxidation-reduction potential data pointwise, be depicted as curve, grasp variation tendency, find optimum point.
6, the hydrogeneous water of plant completing to activate is carried out hydrogen escape rate measurement, within every 15 minutes, surveys once, continuity 15 is little
Time stop, drawing with the time (minute) as abscissa, containing hydrogen density survey C (H2) and hydrogen that ORP current potential is vertical coordinate escape song
Line.
7, write out laboratory report.
8, by dark glass bottle or metal bottle, the plant hydrogen rich water that encapsulation is obtained, preserve in being placed in the refrigerator of 4 DEG C, with
Standby rear use.
9, during whole preparation stock solution and activation produce hydrogen, environment and vessel all necessary strict sterilization, sterilization, prevent miscellaneous
Bacterium pollutes.Operator's personal integrity GMP to be met code requirement.
Beneficial effects of the present invention
What the present invention proposed prepare the method for vegetable active hydrogen water based on biological engineering has an advantage highlighted below:
(1) efficiency is high；The time of the activated plant activation hydrogen that the present invention proposes is shorter, and from several hours to several days, the time was just
Activation process can be completed.Hydrogen technique is produced in contrast, will walk through two steps: the first step needs to prepare culture fluid (1-4 month), second step
Fermentation liquid to be prepared (1-4 month).Total duration was often beyond 1 year.The process of comparison production ferment, the time is longer, generally wants
545 days.Differ tens times to hundred times.
(2) low cost；In addition to decreasing activationary time and human cost, as long as properly selecting raw material, ensureing
On the premise of performance, cost is controlled prepared quite reasonable.We can utilize discarded green of the discarded veterinary antibiotics in kitchen and field
Color crops etc. make substrate, turn waste into wealth, make the agricultural plant hydrogen rich water of low cost.
(3) easy: easily to operate；The process of preparation plant hydrogen rich water, only includes making stock solution and natural fermentation activates former
Liquid the two step.Wherein, laboratory pattern preparation stock solution and and activate stock solution the most fairly simple.The stock solution system that industry manufactures
There are pulverizing and two stages of centrifugation although standby, but there is no great difficulty.Activation process is the most uncomplicated.Micro-fermentation is certainly
So, spontaneously carry out.Industrialized production is that automatization is carried out.After obtaining hydrogen rich water, measuring parameter is also repetitive work.
It is not difficult to operate.
(4) effective: to utilize natural plant raw material, the hydrogen rich water of preparation, in addition to containing abundant reactive hydrogen, also protect
Keep most of active substance that plant is intrinsic.Both Wheat Protein, has again the function of extra-nutrition.Effect contains than single
Hydrogen water to be got well.
(5) environmental protection, safety: raw material is all taken from natural plants, without harmful chemical residual.Micro-sweat will not change
Become the basis of plant, simply excite and strengthen its antioxidation.Hydrogen is to be produced by inside, belongs to the endogenous of plant
Hydrogen, is natural materials, safe to the human body.
(6) stable performance: the reactive hydrogen dissolved in plant hydrogen rich water is sufficiently stable, if allowing plant hydrogen rich water open exposure
In atmosphere, in ten several hours, it has almost no change containing hydrogen concentration and oxidation-reduction potential.As shown in Figure 1.
The anti-oxidation characteristics being not only to analyze various plants that has wide range of applications of the present invention provides a kind of particularly useful
Instrument, also for create the 4th kind give hydrogen methods, preparation plant hydrogen rich water provide theory and practice basis.Utilize the present invention's
Method, can prepare various antioxidant food, beverage, medicine and the cosmetology product taken for the mankind, open the wide world,
Create infinite commercial opportunities.On the other hand, hydrogen rich water is the most also having been widely used." a kind of Hydrogen-rich liquid plant is raw for patent
Long regulator and preparation method and application " (CN102657221 A) utilize potassium borohydride KBH4Preparation hydrogen rich water, only satisfies with hydrogen
With in solution the 0.1% of hydrogen, being equivalent to dissolved hydrogen concentration is 0.02ppm, then adds other nutrients, is prepared as plant regulation
Agent, can be used for spraying various crops, vegetables and fruits and many plants, and effect is especially good.Improve the yield of crops, extend fresh
The freshness date of flower, enhances Semen Tritici aestivi opposing infiltration and assists the ability compeled.Due to this province of plant hydrogen rich water prepared by our method
Just from plant, stable, safety, it is applied to crops, effect may be more notable, and cost can be lower.
The method of " quasi-anaerobism, micro-fermentation " activated plant reactive hydrogen of the present invention, a kind of more meaningful purposes, just
Be possible to move towards modernization for the Chinese medicine that China is traditional, scientific, internationalization provides a kind of potential, the most quantitative
Analytical tool.
Accompanying drawing explanation
Fig. 1 be the hydrogeneous concentration decline curve ratio of plant hydrogen rich water and common hydrogen rich water relatively.
As seen from Figure 1, curve 1 is to represent the dissolved hydrogen concentration of the common hydrogen rich water opened wide over time, 10
In individual hour, hydrogeneous lowering of concentration speed is quickly, by 2nd hour, only about the half of initial value, until 25 hours, dense
Degree almost drops to about the 6% of initial value.Curve 2 is the dissolved hydrogen concentration change in time representing the plant hydrogen rich water opened wide
Change, observed 15 hours, find the sufficiently stable containing hydrogen concentration of plant hydrogen rich water, have almost no change.This characteristic is favourable
In preserving, transporting and use.Certainly, long-term preservation hydrogen rich water yet suffers from hydrogen molecule and escapes out asking of chamber wall all-pervasively
Topic.Therefore, the same with common hydrogen rich water, when longer-term storage and transport, macromolecular material, metal or the glass of densification should be selected
Deng as packaging material.
Detailed description of the invention
Below by way of example explanation further details of to present disclosure.
Embodiment 1, utilizes the Activiation method of Laboratary type, activates and measure antioxidation parameter to 10 kinds of Chinese herbal medicine.
Result is listed in table 1.
Table 1
Embodiment 2, utilizes the Activiation method of Laboratary type, 10 kinds of fruit or vegetable is activated and measured antioxidation ginseng
Number.Result is listed in table 2.
Table 2
Embodiment 3, utilizes the Activiation method of Laboratary type, 10 kinds of grains is activated and measured antioxidation ginseng
Number.Result is listed in table 3.
Table 3
Embodiment 4, utilizes the Activiation method of Laboratary type, to roadside weeds, street corner veteran, discarded green vegetable and unknown miscellaneous flower
Carry out activating and measure antioxidation parameter.
Result is listed in table 4.
Table 4
The method utilizing the present invention to propose is can be seen that by above-described embodiment, can be special by the reactive hydrogen contained by any plant
Property ejects.The reactive hydrogen of this inherence is soluble in water, defines biological hydrogen rich water or claims plant hydrogen rich water.It is a kind of natural
, respond well antioxidant.Hydrogen methods can be given as the 4th kind.As can also be seen from Table 1, it is considered in Chinese medicine
The health preserving tonic valuable, drug effect is good, they are outstanding antioxidants.Such as, Herba Dendrobii, Radix Ginseng, Radix Glycyrrhizae and three
Seven etc..But, also some medical herbs, ordinary, is not expected, and is to have looked down upon them in fact.They have first-class antioxidation
Ability.Fructus Citri junoris that such as Pericarpium Citri Reticulatae or all of Fructus Citri tangerinae section comprise, Fructus Citri grandis, especially their skin, than meat more non-oxidizability.Phase
Instead, Cordyceps this propagandized into value and surpassed the valuable tonic of gold, his oxidation resistance is common.Might as well
Orange peel！Cordyceps please should be got off from altar, also its style.It is not unique, but has its counterpart, it is stated that this year Ministry of Public Health
Do so.Announced no longer to be classified as by Cordyceps senior tonic.From table 2-table 4 it is also seen that various gateway.Its
In some data cause the subversive conclusion with convention phase Bo.Such as, as can be seen from Table 4, green tea overnight contains high work
Property hydrogen and oxidation-reduction potential.This seems to remind us, and green tea overnight is goodies, has lost the most unfortunate.Have tea will drink every
Night tea！The most reasonable.Also find a common trait: when almost all of plant contains maximum concentration reactive hydrogen, all in weak
Acidity or subacidity.This point is different with common hydrogen rich water.Common hydrogen rich water is typically alkalescence or neutrality.Plant hydrogen rich water
Acidity, and the gastroenteric environment of the mankind is similar.Water temperature during activation is 25-35 degree, also close with the body temperature of the mankind.These numerals are seemingly
The condition generating endogenous hydrogen in the condition of explanation formation vegetable active hydrogen and human body is quite similar.Whether this means that plant is rich
Reactive hydrogen in hydrogen water and the material that the source hydrogen in human body is same character.If this conclusion is set up, drink plant hydrogen-rich
Water is equal to supplement, directly to human body, the natural endogenous hydrogen that oxidation resistance is extremely strong.Highly beneficial to health.In a word, I
The preliminary information that obtained by the present invention it appeared that many significant phenomenons, draw many useful inspirations.Necessarily refer to
Go out: owing to being limited by condition during experiment and actual measurement, measuring instrument is the simplest and the crudest, experiment or test environment non-standard
The impact of the unfavorable factors such as change, therefore, the 4 groups of data announced in the description of the invention are the knot of summary exemplary, first
Really, it is understood that there may be certain error even mistake, only for reference.Correct way should be set up the project by country, organizes special messenger, uses
Superior instrument records data accurately in normal conditions.Standard to be formulated, sets up the data base of specification, for research unit,
Enterprise, medical institutions, health care use.The Activiation method of vegetable active hydrogen, except having scientific research value, is also Instructing manufacture
New all kinds of hydrogen-rich products provide foundation and guide.Its range of application is quite varied.
The foregoing is only presently preferred embodiments of the present invention, the present invention can not be limited.All spirit in the present invention and former
Within then, any amendment made, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (5)
1. method and the application preparing vegetable active hydrogen water based on biological engineering, it is characterised in that: first have to preparation containing planting
Liquid-the fermenation raw liquid of thing active component, the preparation method of described fermenation raw liquid has two kinds: laboratory method and industrialization side
Method；Laboratory method-and in drinkable water, put into plant according to a certain percentage, heat, boil 100 degrees Celsius, boil 30 points
Clock, natural cooling, removes impurity, obtains fermenation raw liquid；Industrial method-first pulverized by plant high speed disintegrating machine, adds
A certain proportion of drinkable water, then, separates with high speed centrifuge, after precipitation, takes supernatant, obtain fermenation raw liquid；Use " accurate
Anaerobism and micro-fermentation " technique, make stock solution tepor ferment, activated plant reactive hydrogen, be dissolved in water, it is thus achieved that plant hydrogen rich water, tool
Body way is:
(1), getting out measurand, plant must pollute without pesticide or fertilizer residue, if measurand plant is root block, slightly
Stem, nut and large-scale lignin, first have to pulverize after drying, to the granular size after pulverizing without being strict with, less than 3m
, the leaf of these fresh and dried plants, rhizome, fruit or their powder, using the substrate as stock solution；
(2), regulating laboratory temperature to 25 DEG C, atmospheric pressure is 0.1MPa；All measuring instruments are calibrated, including balance,
Timer, ORP tester, pH tester, jigger, thermometer, dissolved hydrogen concentration measurement instrument etc.；
(3), preparing a marmite, or stainless-steel pan, total measurement (volume) is not less than 600ml, puts into 6 grams of substrates-be equivalent to volume 600ml
1% mass percent；As being dry material, such as Chinese herbal medicine and Folium Camelliae sinensis etc., quality should halve, i.e. 3 grams, 0.5% percent mass
Ratio, then fills drinking water, till filling it up with 600ml, adds hot terrine with slow fire, boils, and stops after decocting 30 minutes, makes and sends out
Ferment stock solution；
(4), preparing a glass graduate heat-resisting, that have 500ml graduation mark, 500ml graduation mark, will apart from rim of a cup about 20 centimeters
The above-mentioned boiled fermenation raw liquid prepared is poured in measuring cup, till filling, does not interspaces, uses food fresh keeping film phonograph seal while hot
Beaker mouth, stand, natural cooling, by temperature from close to 100 degree be reduced to 35 degree time, open rapidly preservative film, pour out a part
Stock solution, until 500ml graduation mark, reserves the space of about 20 centimeters, is full of fresh, clean, warm and humid air, contains in air
There are a small amount of oxygen and natural probiotic bacteria；It is again sealed off, is ready for " quasi-anaerobism, micro-fermentation "；
(5), moving in insulating utensils by the beaker filling stock solution of good seal, thermophore temperature is adjusted to 35 DEG C.Switch on power, open
Beginning activation technology program, keeps the temperature of liquid in glass constant, after being incubated 12 hours, starts to measure the main ginseng of liquid
Number；First sampling 20 milliliters, detects oxidation-reduction potential ORP, if nagative potential, illustrates that activating hydrogen is inspired
Come, the measurement of other parameters can be carried out, otherwise, it is not necessary that carry out the measurement of other parameters；Note surveying at every sub-sampling
During amount, sample will first cool to standard state 25 degrees Celsius, if not yet reaching maximum containing hydrogen concentration, continuing insulation and swashing
Live.Repeat this program once every 4～8 hours, detect and record one group of parameter, including: date, time, water temperature, during activation
Length, pH value, containing hydrogen concentration, oxidation-reduction potential (ORP), until the difference of adjacent twice hydrogeneous concentration data, when change is less than 2%
Stop, being important to measure and whole parameters in record 24 hour that moment of insulation；
(6), the plant hydrogen rich water completing to activate is carried out hydrogen escape rate measurement, within every 15 minutes, surveys once, continue 15 hours
Stop, drawing with the time (minute) as abscissa, survey C (H containing hydrogen density2) and hydrogen escape curve that ORP current potential is vertical coordinate；
(7), by dark glass bottle or metal bottle, the plant hydrogen rich water that encapsulation is obtained, preserve in being placed in the refrigerator of 4 DEG C；
(8), during whole preparation stock solution and activation produce hydrogen, environment and vessel all necessary strict sterilization, sterilization, prevents miscellaneous bacteria
Pollute, operator's personal integrity GMP to be met code requirement.
A kind of method and application preparing vegetable active hydrogen water based on biological engineering the most according to claim 1, its feature
It is: the green crop etc. that the veterinary antibiotics that kitchen can be utilized to discard and field are discarded makes substrate, makes the agriculture of low cost
Use plant hydrogen rich water.
A kind of method and application preparing vegetable active hydrogen water based on biological engineering the most according to claim 1, its feature
It is: during tepor fermentation, thermophore temperature is adjustable to 25 DEG C.
A kind of method and application preparing vegetable active hydrogen water based on biological engineering the most according to claim 1, its feature
Be: during preparation stock solution, put into 3% mass percent of 18 grams of substrates-be equivalent to volume 600ml, as being dry material, such as in
Medical herbs and Folium Camelliae sinensis etc., quality should halve, i.e. 9 grams, 1.5% mass percent.
A kind of method and application preparing vegetable active hydrogen water based on biological engineering the most according to claim 1, its feature
Being: the almost all of plant reactive hydrogen that is activated is when reaching maximum concentration, hydrogen rich water is all in faintly acid or subacidity.
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| WO2022082487A1 (en) * | 2020-10-21 | 2022-04-28 | 朱上翔 | Anti-aging composite nutrient containing coenzymes and bioactive hydrogen, preparation method therefor and application thereof |
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