CN106290943A - A kind of flow cytometer bus control device and method - Google Patents
A kind of flow cytometer bus control device and method Download PDFInfo
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- 238000000034 method Methods 0.000 title claims description 12
- 230000003750 conditioning effect Effects 0.000 claims abstract description 16
- 230000003287 optical effect Effects 0.000 claims abstract description 16
- 230000005540 biological transmission Effects 0.000 claims description 14
- 238000004088 simulation Methods 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 238000000605 extraction Methods 0.000 claims description 10
- 239000004005 microsphere Substances 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 4
- 238000004148 unit process Methods 0.000 claims description 4
- 230000001174 ascending effect Effects 0.000 claims description 3
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- 238000004458 analytical method Methods 0.000 description 2
- 230000005622 photoelectricity Effects 0.000 description 2
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- 210000001367 artery Anatomy 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000013500 data storage Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 238000011527 multiparameter analysis Methods 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
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- G—PHYSICS
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- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1431—Signal processing the electronics being integrated with the analyser, e.g. hand-held devices for on-site investigation
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Abstract
The invention provides a kind of flow cytometer bus control device, it is characterised in that: include sensor unit, data acquisition unit, PCI E interface unit, slave computer interface unit, main control unit and host computer data receipt unit.The optical signal received is converted to the signal of telecommunication according to certain corresponding relation by this sensor unit, the signal of telecommunication that sensor unit is exported by this data acquisition unit realizes conditioning, and convert analog signals into corresponding digital signal, finally the pulse parameter characterizing cell characteristics is extracted, then with the form of PCI E bus protocol by uplink data sending, this PCI E interface unit realizes the topological structure of PCI E bus, the quantity of extension PCI E interface, it is achieved the PCI E bus between main control unit with data acquisition unit and slave computer interface unit is connected.
Description
Technical field
The present invention relates to the bus marco field of flow cytometer, it is proposed that number between a kind of flow cytometer systems module
According to mutual bus mastering mode.
Background technology
The principle of flow cytometer be single cell suspension through specific fluorescence dye dyeing laggard enter streaming exposure cell, at stream
The analysis site of formula exposure cell, laser is irradiated to cell and scattering and refraction occurs, launches scattered light, the fluorescein entrained by cell
It is excited and launches fluorescence, forward scattering light (FSC) and side scattered light (SSC).By electrooptical device by fluorescence and
Scattered light is converted into the signal of telecommunication, and forward scattering light is converted into the signal of telecommunication by photodiode, and side scattered light is by photoelectricity times
Increasing and be converted into the signal of telecommunication, fluorescence is then collected by condenser, and the fluorescence of different colours is turned to different photoelectricity times by double-color reverse light microscopic
Increase pipe detector, fluorescence signal is converted into the signal of telecommunication, scattered light signal and fluorescence signal after amplifying, then through datumization
Processing, input computer also stores, and scattered light and fluorescence according to cell are analyzed.
The degree of modularity of flow cytometer is the highest, have sensor unit, data acquisition unit, PCI-E interface unit, under
Position machine interface unit, main control unit, host computer data receipt unit, unit is each from becoming module, present stage fluidic cell
Instrument multi parameter analysis is fast-developing, analyzes classification speed and accelerates, and the data volume of data acquisition unit is increasing, to bus bandwidth
Having higher requirement, conventional flow cytometer bus uses the parallel bus such as VME, and bus bandwidth is limited, expansible board
Quantity is few, and holding wire is many, and wiring difficulty strengthens.
Accordingly, it would be desirable to a kind of flow cytometer bus mastering mode apparatus and method that can effectively solve the problems referred to above.
Summary of the invention
According to an aspect of the invention, it is provided a kind of flow cytometer bus control device, it is characterised in that described
Bus control device includes multiple data acquisition lists of multiple sensor unit connection corresponding with the plurality of sensor unit
Unit, PCI-E interface unit, slave computer interface unit, main control unit and host computer data receipt unit, wherein, described sensor
The optical signal received is converted to the signal of telecommunication according to certain corresponding relation by unit；Described data acquisition unit is to sensor list
The signal of telecommunication of unit's output realizes conditioning, and converts analog signals into corresponding digital signal, finally to characterizing cell characteristics
Pulse parameter is extracted, then with the form of PCI-E bus protocol by uplink data sending；Described PCI-E interface unit is real
The topological structure of existing PCI-E bus, the quantity of extension PCI-E interface, it is achieved main control unit and data acquisition unit and slave computer
PCI-E bus between interface unit connects；PCI-E Data Format Transform is slave computer bus by described slave computer interface unit
Form realizes the instruction to main control unit and controls；Described main control unit realize to PCI-E bus data transmitting-receiving control and according to
Certain agreement communicates with host computer data receipt unit；Described host computer data receipt unit and main control unit are according to one
Fixed agreement communicates, it is achieved the reception of data and the transmission of various control instruction.
Preferably, described sensor unit is used for scattering relevant to cell or microsphere during flow cytometer detects
Light and fluorescence signal carry out opto-electronic conversion, and feature and intensity according to all kinds of optical signals use different sensors to detect.
Preferably, described data acquisition unit is nursed one's health for the simulation electric impulse signal exporting sensor unit,
It is to can be used for the simulation electric impulse signal that subsequent treatment is analyzed, this data acquisition list by the electric impulse signal conditioning of wide dynamic characteristic
Unit carries out analog/digital conversion to the simulation electric impulse signal after conditioning, and the simulation electric impulse signal after conditioning is converted to numeral arteries and veins
Rushing signal, data acquisition unit carries out calculating process to this digital pulse signal, it is achieved characterize cell or the pulse of microsphere feature
The extraction of parameter.
Preferably, described PCI-E interface unit by main control unit, data acquisition unit and slave computer interface unit according to master
PCI-E bus topolopy is connected into from pattern.
Preferably, the data pulse parameter of each data acquisition unit leaves data buffer unit according to certain format, presses
Mode according to DMA is transferred in the internal memory of main control unit through PCI-E ascending by the PCI-E interface of data acquisition unit；Respectively
The mode that the control command of circuit-switched data collecting unit is read according to internal memory, internal memory is write is by the PCI-E interface warp of data acquisition unit
PCI-E interface unit is transferred to main control unit；The data of described slave computer interface unit are read according to internal memory, the mode write of internal memory by
PCI-E interface in lower computer unit is sent to main control unit through PCI-E interface unit.
Preferably, described main control unit master realizes data acquisition unit in the way of principal and subordinate and slave computer interface unit is straight
The data interaction connect.This main control unit is read by internal memory, internal memory sends to data acquisition unit and slave computer interface in the way of writing
Control command, scattered light and the fluorescence parameter of each data acquisition unit are stored in one piece of company in the way of DMA by main control unit
In continuous internal memory, main control unit processes the data of this block internal memory, and extraction relevant parameter is transferred to host computer data by Ethernet and connects
Receive unit, data acquisition unit, and slave computer interface unit in the way of CPU from message signal interrupt to main control unit propose in
Disconnected, obtain interrupt vector CPU can transfer interrupt service routine is the unit service that request is interrupted.
Preferably, the PCI-E protocol instructions of main control unit is converted to corresponding slave computer by described slave computer interface unit
Protocol instructions, described slave computer protocol instructions mode realize each main control unit 104 control, the instruction such as sensor states monitoring and number
According to transmission.
According to a further aspect in the invention, it is provided that a kind of total for flow cytometer bus control device described above
Line control method, comprises the following steps:
Single sensor unit carries out photoelectric conversion to multipath light signal, and selects difference according to the intensity properties of optical signal
The sensor of type or model is as the detection device of optical signal；
Sensor unit and data acquisition unit one_to_one corresponding, individual data collecting unit can realize and sensor unit
The parameter extraction of the photo-signal channel number that middle number of sensors is identical；
The pulse parameter being stored in data buffer zone that data acquisition unit processes is by PCI-E interface unit and master control list
In the way of DMA, realize data transmission between unit in the way of memory read-write, realize the transmission of control command；
PCI-E interface unit completes the bus topolopy of PCI-E bus with the form of switch；
Main control unit by control command is sent to PCIe slave computer interface, then solves by the way of memory read-write
Analysis.
Flow cytometer bus control device according to the present invention can realize based on three generations's IO bus standard PCIe bus
Control, with the obvious advantage in terms of bandwidth with extensibility.
Should be appreciated that aforementioned description substantially is exemplary illustration and explanation with follow-up detailed description, should not
It is used as the restriction of content claimed to the present invention.
Accompanying drawing explanation
With reference to the accompanying drawing enclosed, as follows by by embodiment of the present invention of the more purpose of the present invention, function and advantage
Description is illustrated, wherein:
Fig. 1 is the structural representation of the flow cytometer bus control device according to the present invention；
Fig. 2 is two kinds of exemplary block diagram of the data acquisition unit according to the present invention；
Fig. 3 is the data acquisition unit PCI-E EBI schematic diagram according to the present invention；
Fig. 4 is the data acquisition unit PCI-E bus marco schematic diagram according to the present invention；
Fig. 5 is many capture cards control flow chart of the main control unit according to the present invention.
Detailed description of the invention
Hereinafter, embodiments of the invention will be described with reference to the drawings.In the accompanying drawings, identical reference represents identical
Or similar parts, or same or similar step.
By with reference to one exemplary embodiment, the purpose of the present invention and function and for realizing the side of these purposes and function
Method will be illustrated.But, the present invention is not limited to one exemplary embodiment disclosed below；Can be come by multi-form
It is realized.The essence of description is only the detail helping the various equivalent modifications Integrated Understanding present invention.
Combine schematic diagram for the present invention to be described in detail, when describing the embodiment of the present invention in detail, for purposes of illustration only, represent
The profile of device architecture can be disobeyed general ratio and be made partial enlargement, and described schematic diagram is example, and it should not limit at this
The scope of protection of the invention processed.Additionally, the three-dimensional space of length, width and the degree of depth should be comprised in actual fabrication.
Fig. 1 is the structural representation of the flow cytometer bus control device according to the present invention, according to the streaming of the present invention
Cell instrument bus control device 100 includes first sensor unit the 101, second sensor unit the 102, the 3rd sensor unit
103, by HDMI physical interface by the first data acquisition unit 105, the second data acquisition unit 106, the 3rd data acquisition list
Unit 107 is connected with first sensor unit the 101, second sensor unit the 102, the 3rd sensor unit 103 respectively.Master control list
Unit's 104, first data acquisition unit the 105, second data acquisition unit the 106, the 3rd data acquisition unit 107 and slave computer connect
Mouthful unit 108 is inserted into PCI-E interface unit 109 by PCI-E interface, and main control unit 104 passes through Ethernet netting twine and upper
Machine data receipt unit 110 connects.
The control based on three generations's IO bus standard PCIe bus of the flow cytometer bus control device of the present invention, at band
Wide with extensibility in terms of with the obvious advantage.
For reaching object above, specifically, first sensor unit the 101, second sensor unit the 102, the 3rd sensor
The optical signal received is converted to the signal of telecommunication according to certain corresponding relation by unit 103 respectively, the first data acquisition unit
105, the second data acquisition unit the 106, the 3rd data acquisition unit 107 is respectively to first sensor unit the 101, second sensor
The signal of telecommunication of unit the 102, the 3rd sensor unit 103 output realizes conditioning, and converts analog signals into corresponding numeral letter
Number, finally the pulse parameter characterizing cell characteristics is extracted, then pulse parameter from each data acquisition unit
PCIe interface uplink.
First data acquisition unit the 105, second data acquisition unit the 106, the 3rd respective PCI-of data acquisition unit 107
E interface realize between main control unit 104 and data acquisition unit and main control unit 104 and slave computer interface unit 108 data and
Instruct the transmission according to PCI-E bus protocol.
PCI-E Data Format Transform is that slave computer bus format realizes main control unit 104 by slave computer interface unit 108
Instruction control, and various feedback informations be converted to PCI-E data form be sent to main control unit 104.
PCI-E interface unit 109 realization switch (Switch) realizes the topological structure of PCI-E interface, extends PCI-E
The quantity of interface, it is achieved the PCI-E bus between main control unit 104 and data acquisition unit and slave computer interface unit 108 is even
Connect.
Main control unit 104 realizes the control to the transmitting-receiving of PCI-E bus data and according to certain agreement and host computer data
Receive unit 110 to communicate.
Host computer data receipt unit 110 communicates according to certain agreement with main control unit 104, it is achieved connecing of data
Receive and the transmission of various control instruction.
PCI-E Data Format Transform is that slave computer bus format realizes master control list by described slave computer interface unit 108
The instruction of unit 104 controls；
Preferably, data acquisition unit is for nursing one's health, by width the simulation electric impulse signal of sensor unit output
The electric impulse signal conditioning of dynamic characteristic is to can be used for the simulation electric impulse signal that subsequent treatment is analyzed, this data acquisition unit pair
Simulation electric impulse signal after conditioning carries out analog/digital conversion, and the simulation electric impulse signal after conditioning is converted to digit pulse letter
Number, data acquisition unit carries out calculating process to this digital pulse signal, it is achieved characterize cell or the pulse parameter of microsphere feature
Extraction.Pulse parameter is left in data buffer storage unit the control instruction according to main control unit 104 by this data acquisition unit
Pulse parameter PCI-E interface on data acquisition unit is transferred to the corresponding data storage areas of main control unit 104.
Preferably, described first data acquisition unit the 105, second data acquisition unit the 106, the 3rd data acquisition unit
107 are used for the scattered light relevant to cell or microsphere and fluorescence signal during flow cytometer detects carries out opto-electronic conversion,
Feature and intensity according to all kinds of optical signals use different sensors to detect.
Generally, scattered light signal can use the most by force the sensor such as photodiode, avalanche diode to detect；Glimmering
Optical signal relatively weak employing photomultiplier tube (PMT) detects.According to the difference of flow cytomery performance, sensor
The possible more than one of unit, above three sensor unit and three corresponding data acquisition units are only the one of the present invention
Individual embodiment.Comprising more than one sensor in each sensor unit, a road optical signal is detected by each sensor.Pass
Sensor cell and subsequent data acquisition unit one_to_one corresponding.
Preferably, PCI-E interface unit 109 is by main control unit 104, data acquisition unit and slave computer interface unit 108
PCI-E bus topolopy is connected into according to master slave mode.Main control unit 104 is that RC end, data acquisition unit and slave computer connect
Mouth unit 108 is EP end, and this PCI-E interface unit 109 can be that a part for main control unit 104 can also individually become one
Absolute construction.
Preferably, the data pulse parameter of each circuit-switched data collecting unit leaves data buffer unit according to certain format,
It is transferred to the internal memory of main control unit 104 through PCI-E ascending by the PCI-E interface of data acquisition unit according to the mode of DMA
In.The mode that the control command of each circuit-switched data collecting unit is read according to internal memory, internal memory is write is by the PCI-E interface of data acquisition unit
It is transferred to main control unit 104 through PCI-E interface unit 109.The data of slave computer interface are read according to internal memory, the mode write of internal memory by
PCI-E interface in lower computer unit is sent to main control unit 104 through PCI-E interface unit 109.
Preferably, main control unit 104 mainly realizes data acquisition unit and slave computer interface unit in the way of principal and subordinate
108 direct data interactions.This main control unit 104 by internal memory read, internal memory write in the way of to data acquisition unit and slave computer
Interface transmitting control commands, scattered light and the fluorescence parameter of each data acquisition unit are deposited in the way of DMA by main control unit 104
It is put into main control unit 104 in one piece of continuous print internal memory and processes the data of this block internal memory, extract relevant parameter and transmitted by Ethernet
To host computer data receipt unit 110.Data acquisition unit, and slave computer interface unit 108 is in the way of message signal interrupt
Proposing to interrupt to the CPU of main control unit 104, obtain interrupt vector CPU can transfer interrupt service routine is the list that request is interrupted
Unit's service.
Preferably, the PCI-E protocol instructions of main control unit 104 is converted to corresponding bottom by slave computer interface unit 108
Machine protocol instructions, conventional slave computer protocol mode have RS232,485, eCan etc., it is right that this slave computer protocol mode mainly realizes
Instruction and the transmission of data such as the control of main control unit 104, sensor states monitoring.
According to a further aspect in the invention, additionally provide a kind of flow cytometer bus control method, comprise the following steps:
Single sensor unit carries out photoelectric conversion to multipath light signal, and selects difference according to the intensity properties of optical signal
The sensor of type or model is as the detection device of optical signal；
Sensor unit and data acquisition unit one_to_one corresponding, individual data collecting unit can realize and sensor unit
The parameter extraction of the photo-signal channel number that middle number of sensors is identical；
The pulse parameter being stored in data buffer zone that data acquisition unit processes is by PCI-E interface unit 109 and master
In the way of DMA, realize data transmission between control unit 104 in the way of memory read-write, realize the transmission of control command；
PCI-E interface unit 109 completes the bus topolopy of PCI-E bus with the form of switch；
Main control unit 104 by the way of memory read-write by control command being sent to PCIe slave computer interface, then
Resolve.
Fig. 2 is two kinds of exemplary block diagram of the data acquisition unit according to the present invention.Wherein in Fig. 2 (a), data acquisition
Unit is sequentially connected with and includes analog/digital conversion module, simulates electric pulse conditioning unit, parameter extraction module and data buffer storage unit；
In Fig. 2 (b), data acquisition unit is sequentially connected with and includes simulating electric pulse conditioning unit, analog-to-digital conversion module, parameter extraction mould
Block, data buffer storage unit.The optical signal received is converted to the signal of telecommunication, data according to certain corresponding relation by sensor unit
The signal of telecommunication that sensor unit is exported by collecting unit realizes conditioning, and converts analog signals into corresponding digital signal, connects
The pulse parameter to characterizing cell characteristics to extract, the parameter of extraction is stored in data buffer storage list according to certain form
Unit, the PCI-E interface module of data acquisition unit with the form of DMA the pulse parameter in buffer unit from EP end PCIe interface
Transmit upstream to control unit.
Fig. 3 is the PCI-E EBI schematic diagram of the data acquisition unit according to the present invention.The PCI-of data acquisition unit
E interface designs in fpga chip, by ram in slice, dma control logic, sends parts, receives the application layer module such as parts, with
And transaction layer, link layer, physical layer are constituted, form a complete PCI-E EBI.
Fig. 4 is the PCI-E bus marco schematic diagram of the data acquisition unit according to the present invention, the PCI-of data acquisition unit
After E interface electrification reset, receive TLP bag, judge the type of TLP bag according to packet header type, if internal memory is write, according to receive
Address, it is judged that be DMA write command or the control command sent to data collecting card, if control command then resolves control life
Make the work of Control card, if command dma, then according to the TLP bag received, parse the destination address of DMA, seedbed
Location, data length, then organize one or more internal memory to write TLP bag according to dma information, by the pulse parameter in data buffer storage
Data send to control unit, and after all of data are sent, the internal memory sending message signal interrupt to control module writes number
According to bag, it is then back to.If TLP reads packet for internal memory, then it is organized into band data after the data of appropriate address being taken out
Complete bag and be sent to main control unit 104, the most directly terminate to return.
After slave computer interface unit 108 electrification reset, receive TLP packet, judge the class of TLP bag according to packet header type
Type, if internal memory is write, according to the address received, to data collecting card transmitting control commands, if internal memory is read then according to connecing
The address received sends the data in slave computer interface unit 108 depositor to main control unit 104, is then back to.
Fig. 5 is many capture cards control flow chart of the main control unit 104 according to the present invention, initializes polylith after system start-up
Capture card unit, then waits host computer unit message, if order then carries out PCI-E write order, or PCI-E reads life
Order, if at this moment port does not has occupied direct execution write operation.If DMA to be performed reads, the most directly control program and send phase
Close command dma, open completing of interrupt latency data acquisition unit dma operation simultaneously.If port read command is the most directly sent out
Sending read command, then wait band data completes packet.Interrupting if receiving PCI-E, the most clear interruption, then processing phase
Close interrupt service routine, return after completing.
Host computer data receipt unit 110 communicates according to certain agreement with main control unit 104, it is achieved connecing of data
Receive and the transmission of various control instruction.
Flow cytometer bus control device according to the present invention can realize based on three generations's IO bus standard PCIe bus
Control, with the obvious advantage in terms of bandwidth with extensibility.
In conjunction with explanation and the practice of the present invention disclosed here, other embodiments of the present invention are for those skilled in the art
All it is easy to expect and understand.Illustrating and embodiment is to be considered only as exemplary, true scope and the purport of the present invention are equal
It is defined in the claims.
Claims (8)
1. a flow cytometer bus control device, it is characterised in that described bus control device includes multiple sensor list
Unit multiple data acquisition units of connection corresponding with the plurality of sensor unit, PCI-E interface unit, slave computer interface list
Unit, main control unit and host computer data receipt unit, wherein,
The optical signal received is converted to the signal of telecommunication according to certain corresponding relation by described sensor unit；
The signal of telecommunication that sensor unit is exported by described data acquisition unit realizes conditioning, and converts analog signals into corresponding
Digital signal, finally extracts the pulse parameter characterizing cell characteristics, then with the form of PCI-E bus protocol by up
Send data；
Described PCI-E interface unit realizes the topological structure of PCI-E bus, the quantity of extension PCI-E interface, it is achieved main control unit
It is connected with the PCI-E bus between data acquisition unit and slave computer interface unit；
PCI-E Data Format Transform is the slave computer bus format realization instruction to main control unit by described slave computer interface unit
Control；
Described main control unit realizes the control to the transmitting-receiving of PCI-E bus data and according to certain agreement and host computer data receiver
Unit communicates；
Described host computer data receipt unit communicates according to certain agreement with main control unit, it is achieved the reception of data and each
Plant the transmission of control instruction.
Flow cytometer bus control device the most according to claim 1, it is characterised in that: described sensor unit is used for
During flow cytometer detection, the scattered light relevant to cell or microsphere and fluorescence signal carry out opto-electronic conversion, according to all kinds of
The feature of optical signal and intensity use different sensors to detect.
Flow cytometer bus control device the most according to claim 2, it is characterised in that: described data acquisition unit is used
Nurse one's health in the simulation electric impulse signal that sensor unit is exported, be available by the electric impulse signal conditioning of wide dynamic characteristic
In subsequent treatment analyze simulation electric impulse signal, this data acquisition unit to conditioning after simulation electric impulse signal carry out mould/
Number conversion, is converted to digital pulse signal by the simulation electric impulse signal after conditioning, and this digit pulse is believed by data acquisition unit
Number carry out calculating process, it is achieved characterize the extraction of the pulse parameter of cell or microsphere feature.
Flow cytometer bus control device the most according to claim 1, it is characterised in that: described PCI-E interface unit
Main control unit, data acquisition unit and slave computer interface unit are connected into PCI-E bus topolopy according to master slave mode.
Flow cytometer bus control device the most according to claim 1, it is characterised in that: the number of each data acquisition unit
Leave data buffer unit according to pulse parameter according to certain format, connect by the PCI-E of data acquisition unit according to the mode of DMA
Mouth is transferred in the internal memory of main control unit through PCI-E ascending；The control command of each circuit-switched data collecting unit according to internal memory read,
The mode that internal memory is write is transferred to main control unit by the PCI-E interface of data acquisition unit through PCI-E interface unit；Described slave computer
The data of interface unit are read according to internal memory, the mode write of internal memory by the PCI-E interface in lower computer unit through PCI-E interface unit
It is sent to main control unit.
Flow cytometer bus control device the most according to claim 1, it is characterised in that: described main control unit master is with master
From mode realize data acquisition unit and the direct data interaction of slave computer interface unit.This main control unit with internal memory read,
The mode that internal memory is write is to data acquisition unit and slave computer interface transmitting control commands, and main control unit will be each in the way of DMA
The scattered light of individual data acquisition unit and fluorescence parameter are stored in main control unit in one piece of continuous print internal memory and process this block internal memory
Data, extract relevant parameter and are transferred to host computer data receipt unit, data acquisition unit, and slave computer interface by Ethernet
Unit proposes to interrupt to the CPU of main control unit in the way of message signal interrupt, obtains interrupt vector CPU and can transfer interruption clothes
Business program is the unit service that request is interrupted.
Flow cytometer bus control device the most according to claim 1, it is characterised in that: described slave computer interface unit
The PCI-E protocol instructions of main control unit is converted to corresponding slave computer protocol instructions, and described slave computer protocol instructions mode is real
Instruction and the transmission of data such as the most each main control unit 104 controls, sensor states monitoring.
8. for a bus control method for flow cytometer bus control device as claimed in claim 1, including following
Step:
Single sensor unit carries out photoelectric conversion to multipath light signal, and selects dissimilar according to the intensity properties of optical signal
Or the sensor of model is as the detection device of optical signal；
Sensor unit and data acquisition unit one_to_one corresponding, individual data collecting unit can realize passing in sensor unit
The parameter extraction of the photo-signal channel number that sensor quantity is identical；
The pulse parameter being stored in data buffer zone that data acquisition unit processes by PCI-E interface unit and main control unit it
Between in the way of DMA, realize data transmission in the way of memory read-write, realize the transmission of control command；
PCI-E interface unit completes the bus topolopy of PCI-E bus with the form of switch；
Main control unit by control command is sent to PCIe slave computer interface, then resolves by the way of memory read-write.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2015106621448 | 2015-10-14 | ||
| CN201510662144 | 2015-10-14 |
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| CN106290943A true CN106290943A (en) | 2017-01-04 |
| CN106290943B CN106290943B (en) | 2018-07-27 |
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| CN201610890460.5A Active CN106290943B (en) | 2015-10-14 | 2016-10-12 | A kind of flow cytometer bus control device and method |
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