CN106290943B - A kind of flow cytometer bus control device and method - Google Patents

A kind of flow cytometer bus control device and method Download PDF

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Publication number
CN106290943B
CN106290943B CN201610890460.5A CN201610890460A CN106290943B CN 106290943 B CN106290943 B CN 106290943B CN 201610890460 A CN201610890460 A CN 201610890460A CN 106290943 B CN106290943 B CN 106290943B
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data
pci
main control
memory
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CN106290943A (en
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娄小平
祝连庆
张文昌
刘锋
郭阳宽
董明利
孟晓辰
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Beijing Information Science and Technology University
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584Control arrangements for automatic analysers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1425Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429Signal processing
    • G01N15/1431Signal processing the electronics being integrated with the analyser, e.g. hand-held devices for on-site investigation

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  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The present invention provides a kind of flow cytometer bus control devices, it is characterised in that:Including sensor unit, data acquisition unit, PCI E interfaces unit, slave computer interface unit, main control unit and host computer data receipt unit.The optical signal received is converted to electric signal by the sensor unit according to certain correspondence, the data acquisition unit realizes conditioning to the electric signal that sensor unit exports, and convert analog signals into corresponding digital signal, finally the pulse parameter for characterizing cell characteristics is extracted, then by uplink data sending in the form of PCI E bus protocols, the PCI E interface units realize the topological structure of PCI E buses, the quantity of PCI E interfaces is extended, realizes that the PCI E buses between main control unit and data acquisition unit and slave computer interface unit connect.

Description

A kind of flow cytometer bus control device and method
Technical field
The present invention relates to the bus marco fields of flow cytometer, it is proposed that number between a kind of flow cytometer systems module According to interactive bus mastering mode.
Background technology
The principle of flow cytometer is that single cell suspension enters streaming exposure cell after specific fluorescence dye dyes, and is being flowed The analysis site of formula exposure cell, laser irradiation to cell occur scattering and refraction, launch scattering light, the fluorescein entrained by cell It is excited and launches fluorescence, forward scattering light (FSC) and side scattered light (SSC).By electrooptical device by fluorescence and Scattering light is converted into electric signal, and forward scattering light is converted into electric signal by photodiode, and side scattered light passes through photoelectricity times Increasing is converted into electric signal, and fluorescence is then collected by concentrator, and the fluorescence of different colours turns to different photoelectricity times by double-colored reflective mirror Increase pipe detector, fluorescence signal is converted into electric signal, scattered light signal and fluorescence signal are after amplification, using digitization Processing inputs computer and stores, analyzed according to the scattering light and fluorescence of cell.
The degree of modularity of flow cytometer is very high, have sensor unit, data acquisition unit, PCI-E interface unit, under Position machine interface unit, main control unit, host computer data receipt unit, the respective self-contained module of each unit, at this stage fluidic cell Instrument multi parameter analysis is fast-developing, and analysis classification speed is accelerated, and the data volume of data acquisition unit is increasing, to bus bandwidth More stringent requirements are proposed, and conventional flow cytometer bus is using parallel bus such as VME, and bus bandwidth is limited, expansible board Quantity is few, and signal wire is more, and wiring difficulty increases.
Therefore, it is necessary to a kind of flow cytometer bus mastering mode device and method that can effectively solve the above problems.
Invention content
According to an aspect of the invention, there is provided a kind of flow cytometer bus control device, which is characterized in that described Bus control device includes multiple sensor units, the multiple data being correspondingly connected with the multiple sensor unit acquisition list Member, PCI-E interface unit, slave computer interface unit, main control unit and host computer data receipt unit, wherein the sensor The optical signal received is converted to electric signal by unit according to certain correspondence;The data acquisition unit is to sensor list The electric signal of member output realizes conditioning, and converts analog signals into corresponding digital signal, finally to characterization cell characteristics Pulse parameter extracts, then by uplink data sending in the form of PCI-E bus protocols;The PCI-E interface unit is real The topological structure of existing PCI-E buses, extends the quantity of PCI-E interface, realizes main control unit and data acquisition unit and slave computer PCI-E buses connection between interface unit;PCI-E Data Format Transforms are slave computer bus by the slave computer interface unit Format realizes that the instruction to main control unit controls;The main control unit realize the control received and dispatched to PCI-E bus datas and according to Certain agreement is communicated with host computer data receipt unit;The host computer data receipt unit is with main control unit according to one Fixed agreement is communicated, and realizes the reception of data and the transmission of various control instructions.
Preferably, the sensor unit is used in flow cytometer detection process and cell or the relevant scattering of microballoon Light and fluorescence signal carry out opto-electronic conversion, are detected using different sensors according to the feature of all kinds of optical signals and intensity.
Preferably, the data acquisition unit is used to improve the simulation electric impulse signal that sensor unit exports, By the electric impulse signal conditioning of wide dynamic characteristic to can be used for the simulation electric impulse signal of subsequent processing analysis, data acquisition is single Member carries out analog/digital conversion to the simulation electric impulse signal after conditioning, and the simulation electric impulse signal after conditioning is converted to digital arteries and veins Signal is rushed, data acquisition unit carries out calculation processing to the digital pulse signal, realizes the pulse of characterization cell or microballoon feature The extraction of parameter.
Preferably, the PCI-E interface unit by main control unit, data acquisition unit and slave computer interface unit according to master Slave pattern connects into PCI-E bus topolopies.
Preferably, the data pulse parameter of each data acquisition unit is stored in data buffer unit according to certain format, presses It is transferred in the memory of main control unit through PCI-E ascendings by the PCI-E interface of data acquisition unit according to the mode of DMA;Respectively The control command of circuit-switched data collecting unit is passed through in such a way that memory is read, memory is write by the PCI-E interface of data acquisition unit PCI-E interface unit is transferred to main control unit;The data of the slave computer interface unit by memory read, memory write in the way of by PCI-E interface in lower computer unit is sent to main control unit through PCI-E interface unit.
Preferably, the main control unit master realizes that data acquisition unit and slave computer interface unit are straight in a manner of principal and subordinate The data interaction connect.The main control unit is sent in such a way that memory is read, memory is write to data acquisition unit and slave computer interface The scattering light and fluorescence parameter of each data acquisition unit are stored in one piece of company by control command, main control unit in a manner of DMA Main control unit handles the data of this block memory in continuous memory, and extraction relevant parameter is transferred to host computer data by Ethernet and connects Unit is received, during data acquisition unit and slave computer interface unit are proposed in a manner of message signal interrupt to the CPU of main control unit It is disconnected, it obtains interrupt vector CPU and can transfer interrupt service routine to be the unit service that request is interrupted.
Preferably, the PCI-E protocol instructions of main control unit are converted to corresponding slave computer by the slave computer interface unit Protocol instructions, the slave computer protocol instructions mode realize instruction and the numbers such as the control of each main control unit 104, sensor states monitoring According to transmission.
According to another aspect of the present invention, it provides a kind of for the total of such as above-mentioned flow cytometer bus control device Line control method includes the following steps:
Single sensor unit carries out photoelectric conversion to multipath light signal, and selects difference according to the intensity properties of optical signal Detection device of the sensor of type or model as optical signal;
Sensor unit is corresponded with data acquisition unit, and individual data collecting unit may be implemented and sensor unit The parameter extraction of the identical photo-signal channel number of middle number of sensors;
The pulse parameter for being stored in data buffer zone of data acquisition unit processing passes through PCI-E interface unit and master control list Realize that data transmission realizes the transmission of control command in a manner of memory read-write in a manner of DMA between member;
PCI-E interface unit completes the bus topolopy of PCI-E buses in the form of interchanger;
Main control unit, by the way that control command is transmitted to PCIe slave computer interfaces, is then solved by way of memory read-write Analysis.
Flow cytometer bus control device according to the present invention may be implemented to be based on three generations's IO bus standard PCIe buses Control, it is with the obvious advantage in terms of bandwidth with scalability.
It should be appreciated that aforementioned description substantially and follow-up description in detail are exemplary illustration and explanation, it should not As the limitation to the claimed content of the present invention.
Description of the drawings
With reference to the attached drawing of accompanying, the more purposes of the present invention, function and advantage are by the as follows of embodiment through the invention Description is illustrated, wherein:
Fig. 1 is the structural schematic diagram according to the flow cytometer bus control device of the present invention;
Fig. 2 is two kinds of exemplary block diagrams according to the data acquisition unit of the present invention;
Fig. 3 is the data acquisition unit PCI-E bus interface schematic diagrames according to the present invention;
Fig. 4 is the data acquisition unit PCI-E bus marco schematic diagrames according to the present invention;
Fig. 5 is more capture card control flow charts according to the main control unit of the present invention.
Specific implementation mode
Hereinafter, the embodiment of the present invention will be described with reference to the drawings.In the accompanying drawings, identical reference numeral represents identical Or similar component or same or like step.
By reference to exemplary embodiment, the purpose of the present invention and function and the side for realizing these purposes and function Method will be illustrated.However, the present invention is not limited to exemplary embodiment as disclosed below;Can by different form come It is realized.The essence of specification is only to aid in the detail of the various equivalent modifications Integrated Understanding present invention.
It is described in detail for combination schematic diagram of the present invention, when describing the embodiments of the present invention, for purposes of illustration only, indicating The sectional view of device architecture can disobey general proportion and make partial enlargement, and the schematic diagram is example, should not be limited herein The scope of protection of the invention processed.In addition, three-dimensional space that should be comprising length, width and depth in actual fabrication.
Fig. 1 is the structural schematic diagram of flow cytometer bus control device according to the present invention, streaming according to the present invention Cell instrument bus control device 100 includes first sensor unit 101, second sensor unit 102,3rd sensor unit 103, by HDMI physical interfaces by the first data acquisition unit 105, the second data acquisition unit 106, third data acquire single Member 107 is connected with first sensor unit 101, second sensor unit 102,3rd sensor unit 103 respectively.Master control list First 104, first data acquisition unit 105, the second data acquisition unit 106, third data acquisition unit 107 and slave computer connect Mouthful unit 108 is inserted into PCI-E interface unit 109 by PCI-E interface, and main control unit 104 passes through ethernet netting twine and upper Machine data receipt unit 110 connects.
The control based on three generations's IO bus standard PCIe buses of the flow cytometer bus control device of the present invention, in band It is wide with scalability in terms of it is with the obvious advantage.
To achieve the above objectives, specifically, first sensor unit 101, second sensor unit 102,3rd sensor The optical signal received is converted to electric signal, the first data acquisition unit by unit 103 according to certain correspondence respectively 105, the second data acquisition unit 106, third data acquisition unit 107 are respectively to first sensor unit 101, second sensor The electric signal that unit 102,3rd sensor unit 103 export realizes conditioning, and converts analog signals into corresponding number letter Number, finally the pulse parameter for characterizing cell characteristics is extracted, then pulse parameter from each data acquisition unit PCIe interface uplink.
First data acquisition unit 105, the second data acquisition unit 106,107 respective PCI- of third data acquisition unit E interface realize between main control unit 104 and data acquisition unit and main control unit 104 and slave computer interface unit 108 data and Instruct the transmission according to PCI-E bus protocols.
PCI-E Data Format Transforms are that slave computer bus format is realized to main control unit 104 by slave computer interface unit 108 Instruction control, and various feedback informations are converted into PCI-E data formats and are transmitted to main control unit 104.
PCI-E interface unit 109 realizes the topological structure that PCI-E interface is realized with interchanger (Switch), extends PCI-E The quantity of interface realizes that the PCI-E buses between main control unit 104 and data acquisition unit and slave computer interface unit 108 connect It connects.
Main control unit 104 realizes the control received and dispatched to PCI-E bus datas and according to certain agreement and host computer data Receiving unit 110 is communicated.
Host computer data receipt unit 110 is communicated with main control unit 104 according to certain agreement, realizes connecing for data The transmission of receipts and various control instructions.
PCI-E Data Format Transforms are that slave computer bus format is realized to master control list by the slave computer interface unit 108 The instruction control of member 104;
Preferably, data acquisition unit, will be wide for improving the simulation electric impulse signal that sensor unit exports The electric impulse signal conditioning of dynamic characteristic is that can be used for the simulation electric impulse signal of subsequent processing analysis, the data acquisition unit pair Simulation electric impulse signal after conditioning carries out analog/digital conversion, and the simulation electric impulse signal after conditioning is converted to digit pulse letter Number, data acquisition unit carries out calculation processing to the digital pulse signal, realizes the pulse parameter of characterization cell or microballoon feature Extraction.Pulse parameter is stored in data buffer storage unit and according to the control instruction of main control unit 104 by the data acquisition unit Pulse parameter is transferred to 104 corresponding data storage areas of main control unit through the PCI-E interface on data acquisition unit.
Preferably, first data acquisition unit 105, the second data acquisition unit 106, third data acquisition unit 107 are used for carrying out opto-electronic conversion with the relevant scattering light of cell or microballoon and fluorescence signal in flow cytometer detection process, It is detected using different sensors according to the feature of all kinds of optical signals and intensity.
It is detected in general, the sensors such as photodiode, avalanche diode relatively can be used by force in scattered light signal;It is glimmering Optical signal is relatively weak to be detected using photomultiplier (PMT).According to the difference of flow cytomery performance, sensor The possible more than one of unit, above three sensor unit and corresponding three data acquisition units are also only the one of the present invention A embodiment.Include more than one sensor in each sensor unit, each sensor is detected optical signal all the way.It passes Sensor cell is corresponded with subsequent data acquisition unit.
Preferably, PCI-E interface unit 109 is by main control unit 104, data acquisition unit and slave computer interface unit 108 PCI-E bus topolopies are connected into according to master slave mode.Main control unit 104 is the ends RC, and data acquisition unit and slave computer connect Mouth unit 108 is the ends EP, which, which can be a part for main control unit 104, can also individually become one Absolute construction.
Preferably, the data pulse parameter of each circuit-switched data collecting unit is stored in data buffer unit according to certain format, It is transferred to the memory of main control unit 104 through PCI-E ascendings by the PCI-E interface of data acquisition unit in the way of DMA In.The control command of each circuit-switched data collecting unit is in such a way that memory is read, memory is write by the PCI-E interface of data acquisition unit It is transferred to main control unit 104 through PCI-E interface unit 109.The data of slave computer interface by memory read, memory write in the way of by PCI-E interface in lower computer unit is sent to main control unit 104 through PCI-E interface unit 109.
Preferably, main control unit 104 mainly realizes data acquisition unit and slave computer interface unit in a manner of principal and subordinate 108 direct data interactions.The main control unit 104 is in such a way that memory is read, memory is write to data acquisition unit and slave computer Interface sends control command, and main control unit 104 is deposited the scattering light and fluorescence parameter of each data acquisition unit in a manner of DMA The data that main control unit 104 in one piece of continuous memory handles this block memory are put into, extraction relevant parameter is transmitted by Ethernet To host computer data receipt unit 110.Data acquisition unit and slave computer interface unit 108 are in a manner of message signal interrupt It proposes to interrupt to the CPU of main control unit 104, obtains interrupt vector CPU and can transfer interrupt service routine to be the list that request is interrupted Meta-service.
Preferably, the PCI-E protocol instructions of main control unit 104 are converted to corresponding bottom by slave computer interface unit 108 Machine protocol instructions, common slave computer protocol mode have RS232,485, eCan etc., which mainly realizes pair Instructions and the transmission of data such as control, the sensor states monitoring of main control unit 104.
According to another aspect of the present invention, a kind of flow cytometer bus control method is additionally provided, is included the following steps:
Single sensor unit carries out photoelectric conversion to multipath light signal, and selects difference according to the intensity properties of optical signal Detection device of the sensor of type or model as optical signal;
Sensor unit is corresponded with data acquisition unit, and individual data collecting unit may be implemented and sensor unit The parameter extraction of the identical photo-signal channel number of middle number of sensors;
The pulse parameter for being stored in data buffer zone of data acquisition unit processing passes through PCI-E interface unit 109 and master Realize that data transmission realizes the transmission of control command in a manner of memory read-write in a manner of DMA between control unit 104;
PCI-E interface unit 109 completes the bus topolopy of PCI-E buses in the form of interchanger;
Main control unit 104 by way of memory read-write by the way that control command is transmitted to PCIe slave computer interfaces, then Parsing.
Fig. 2 is two kinds of exemplary block diagrams of data acquisition unit according to the present invention.In wherein Fig. 2 (a), data acquisition Unit is sequentially connected including analog/digital conversion module, simulates electric pulse conditioning unit, parameter extraction module and data buffer storage unit; In Fig. 2 (b), data acquisition unit is sequentially connected including simulating electric pulse conditioning unit, analog-to-digital conversion module, parameter extraction mould Block, data buffer storage unit.The optical signal received is converted to electric signal, data by sensor unit according to certain correspondence Collecting unit realizes conditioning to the electric signal that sensor unit exports, and converts analog signals into corresponding digital signal, connects It and the pulse parameter for characterizing cell characteristics is extracted, the parameter of extraction is stored in data buffer storage list according to certain format Member, the PCI-E interface module of data acquisition unit is in the form of DMA the pulse parameter in buffer unit from the ends EP PCIe interface Transmit upstream to control unit.
Fig. 3 is the PCI-E bus interface schematic diagrames of data acquisition unit according to the present invention.The PCI- of data acquisition unit E interface designs in fpga chip, and by ram in slice, dma control logic sends component, the application layer modules such as receiving part, with And transaction layer, link layer, physical layer are constituted, and form a complete PCI-E bus interface.
Fig. 4 is according to the PCI-E bus marco schematic diagrames of the data acquisition unit of the present invention, the PCI- of data acquisition unit After E interface electrification reset, TLP packets are received, the type of TLP packets is judged according to packet header type, is write if it is memory, according to reception Address, judgement are DMA write commands or the control command that is sent to data collecting card, and control life is then parsed if it is control command The work for enabling Control card, if it is command dma, then according to the TLP packets received, with parsing destination address, the source of DMA Location, data length, then according to dma information tissue, one or more memory writes TLP packets, by the pulse parameter in data buffer storage Data are sent to control unit, and after all data are sent, the memory that message signal interrupt is sent to control module writes number According to packet, it is then back to.If TLP, which is memory, reads data packet, it is organized into data after the data of appropriate address are taken out It completes packet and is sent to main control unit 104, then directly terminate to return.
After 108 electrification reset of slave computer interface unit, TLP data packets are received, the class of TLP packets is judged according to packet header type Type is write if it is memory, and according to the address of reception, control command is sent to data collecting card, and reading then basis if it is memory connects The address received sends the data in 108 register of slave computer interface unit to main control unit 104, is then back to.
Fig. 5 is according to more capture card control flow charts of the main control unit 104 of the present invention, and system initializes polylith after starting Then capture card unit waits for host computer unit message, then carry out PCI-E write orders if it is order or PCI-E reads life It enables, at this moment if port does not have occupied direct execution write operation.If executing DMA readings, directly controls program and send phase Command dma is closed, the completion of interrupt latency data acquisition unit dma operation is opened simultaneously.It is then directly sent out if it is port read command Read command is sent, the completion data packet with data is then waited for.It is directly clear if receiving PCI-E and interrupting to interrupt, then handle phase Interrupt service routine is closed, is returned after the completion.
Host computer data receipt unit 110 is communicated with main control unit 104 according to certain agreement, realizes connecing for data The transmission of receipts and various control instructions.
Flow cytometer bus control device according to the present invention may be implemented to be based on three generations's IO bus standard PCIe buses Control, it is with the obvious advantage in terms of bandwidth with scalability.
Explanation in conjunction with the present invention disclosed here and practice, the other embodiment of the present invention is for those skilled in the art It all will be readily apparent and understand.Illustrate and embodiment is regarded only as being exemplary, true scope of the invention and purport are equal It is defined in the claims.

Claims (8)

1. a kind of flow cytometer bus control device, which is characterized in that the bus control device includes multiple sensor lists Member, multiple data acquisition units, PCI-E interface unit, the slave computer to connect one to one with the multiple sensor unit connect Mouth unit, main control unit and host computer data receipt unit, wherein
The optical signal received is converted to electric signal by the sensor unit according to certain correspondence;
The data acquisition unit includes sequentially connected analog/digital conversion module, simulation electric pulse conditioning unit, parameter extraction mould Block and data buffer storage unit, the data acquisition unit realizes conditioning to the electric signal that sensor unit exports, and simulation is believed Number be converted to corresponding digital signal, finally the pulse parameter for characterizing cell characteristics extracted, by the parameter of extraction according to Certain format is stored in data buffer unit, then by uplink data sending in the form of PCI-E bus protocols;
The PCI-E interface unit realizes the topological structure of PCI-E buses, extends the quantity of PCI-E interface, realizes main control unit PCI-E buses between data acquisition unit and slave computer interface unit are connect;
PCI-E Data Format Transforms are instruction of the slave computer bus format realization to main control unit by the slave computer interface unit Control, wherein after the PCI-E interface electrification reset of data acquisition unit, receive TLP packets, TLP packets are judged according to packet header type Type;
It is write if it is memory, according to the address of reception, judgement is DMA write command or the control sent to data collecting card life It enables, the work of control command Control card is then parsed if it is control command, if it is command dma, then according to the TLP received Packet, parses destination address, source address, the data length of DMA, then one or more memory is write according to dma information tissue TLP packets send the pulse parameter data in data buffer storage to control unit, after all data are sent, to control mould Block sends the memory data packets of message signal interrupt, is then back to;
If TLP, which is memory, reads data packet, it is organized into the completion packet with data after the data of appropriate address are taken out and sends To main control unit, then directly terminate to return;
After slave computer interface unit electrification reset, TLP data packets are received, the type of TLP packets is judged according to packet header type;
It is write if it is memory, according to the address of reception, control command is sent to data collecting card, reading then basis if it is memory connects The address received sends the data in slave computer interface unit register to main control unit, is then back to;
The main control unit realizes the control received and dispatched to PCI-E bus datas and according to certain agreement and host computer data receiver Unit is communicated;
The host computer data receipt unit is communicated with main control unit according to certain agreement, realizes the reception of data and each The transmission of kind control instruction.
2. flow cytometer bus control device according to claim 1, it is characterised in that:The sensor unit is used for Opto-electronic conversion is carried out with the relevant scattering light of cell or microballoon and fluorescence signal in flow cytometer detection process, according to all kinds of The feature and intensity of optical signal are detected using different sensors.
3. flow cytometer bus control device according to claim 2, it is characterised in that:The data acquisition unit is used It is improved in the simulation electric impulse signal exported to sensor unit, is available by the electric impulse signal conditioning of wide dynamic characteristic In subsequent processing analysis simulation electric impulse signal, the data acquisition unit to after conditioning simulation electric impulse signal carry out mould/ Simulation electric impulse signal after conditioning is converted to digital pulse signal by number conversion, and data acquisition unit believes the digit pulse Number carry out calculation processing, realize characterization cell or microballoon feature pulse parameter extraction.
4. flow cytometer bus control device according to claim 1, it is characterised in that:The PCI-E interface unit Main control unit, data acquisition unit and slave computer interface unit are connected into PCI-E bus topolopies according to master slave mode.
5. flow cytometer bus control device according to claim 1, it is characterised in that:The number of each data acquisition unit It is stored in data buffer unit according to certain format according to pulse parameter, is connect by the PCI-E of data acquisition unit in the way of DMA Mouth is transferred to through PCI-E ascendings in the memory of main control unit;The control command of each circuit-switched data collecting unit according to memory read, The mode that memory is write is transferred to main control unit by the PCI-E interface of data acquisition unit through PCI-E interface unit;The slave computer The data of interface unit are in such a way that memory is read, memory is write by the PCI-E interface in lower computer unit through PCI-E interface unit It is sent to main control unit.
6. flow cytometer bus control device according to claim 1, it is characterised in that:The main control unit master is with master From mode realize data acquisition unit and the direct data interaction of slave computer interface unit, which is read with memory, The mode that memory is write sends control command to data acquisition unit and slave computer interface, and main control unit will be each in a manner of DMA The scattering light and fluorescence parameter of a data acquisition unit are stored in main control unit in one piece of continuous memory and handle this block memory Data, extraction relevant parameter are transferred to host computer data receipt unit, data acquisition unit and slave computer interface by Ethernet Unit proposes to interrupt in a manner of message signal interrupt to the CPU of main control unit, and interruption clothes can be transferred by obtaining interrupt vector CPU Business program is the unit service that request is interrupted.
7. flow cytometer bus control device according to claim 1, it is characterised in that:The slave computer interface unit The PCI-E protocol instructions of main control unit are converted into corresponding slave computer protocol instructions, the slave computer protocol instructions mode is real Instructions and the transmission of data such as the now control of each main control unit 104, sensor states monitoring.
8. a kind of bus control method for flow cytometer bus control device as described in claim 1, including it is following Step:
Single sensor unit carries out photoelectric conversion to multipath light signal, and selects different type according to the intensity properties of optical signal Or detection device of the sensor of model as optical signal;
Sensor unit is corresponded with data acquisition unit, and individual data collecting unit may be implemented to pass with sensor unit The parameter extraction of the identical photo-signal channel number of sensor quantity;
Data acquisition unit processing be stored in the pulse parameter of data buffer zone by PCI-E interface unit and main control unit it Between realize that data transmission realizes the transmission of control command in a manner of memory read-write in a manner of DMA;
PCI-E interface unit completes the bus topolopy of PCI-E buses in the form of interchanger;
Main control unit, by the way that control command is transmitted to PCIe slave computer interfaces, is then parsed by way of memory read-write;Under PCI-E Data Format Transforms are that slave computer bus format realizes that the instruction to main control unit controls by position machine interface unit, wherein After the PCI-E interface electrification reset of data acquisition unit, TLP packets are received, the type of TLP packets is judged according to packet header type;
It is write if it is memory, according to the address of reception, judgement is DMA write command or the control sent to data collecting card life It enables, the work of control command Control card is then parsed if it is control command, if it is command dma, then according to the TLP received Packet, parses destination address, source address, the data length of DMA, then one or more memory is write according to dma information tissue TLP packets send the pulse parameter data in data buffer storage to control unit, after all data are sent, to control mould Block sends the memory data packets of message signal interrupt, is then back to;
If TLP, which is memory, reads data packet, it is organized into the completion packet with data after the data of appropriate address are taken out and sends To main control unit, then directly terminate to return;
After slave computer interface unit electrification reset, TLP data packets are received, the type of TLP packets is judged according to packet header type;
It is write if it is memory, according to the address of reception, control command is sent to data collecting card, reading then basis if it is memory connects The address received sends the data in slave computer interface unit register to main control unit, is then back to.
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