A kind of anti-saline and alkaline microbial manure and application
Technical field
The present invention relates to a kind of anti-saline and alkaline microbial manure and application, belong to agricultural microbial manure technical field.
Background technology
Salt-soda soil refers to that salinity contained in soil has influence on the salt aggregation soil of plant growth.According to associating state religion
Section's literary composition tissue and the incomplete statistics of the World Food Programme, global salt-soda soil area alreadys more than 9.5 hundred million hectares, wherein,
China has just reached 99,130,000 hectares.China's alkaline earth and the formation of alkali-affected soil, the accumulation of the carbonate in major part and soil
Relevant, salinization and alkalization is the highest, and serious salt affected soil regional plant is little to existence.
Although currently available technology discloses some resist saline and alkaline fertilizer, but during these chemical fertilizer, although
Effect is obvious in a short time, but the chemical fertilizer fertile phase itself is short, loses efficacy fast, and fertility persistency is poor, simultaneously can be original to ecological environment
The most fragile alkaline land soil produces secondary chemical contamination, and, consumption is big, easily causes soil compaction.
Summary of the invention
For solving above-mentioned technical problem, the invention provides a kind of anti-saline and alkaline microbial manure, the technical scheme taked
As follows:
It is an object of the invention to provide a kind of strain for preparing anti-saline and alkaline microbial manure, it is characterised in that institute
State strain and carry out penicillium (Penicillium bilaiae) for ratio, on July 20th, 2016, be preserved in and be positioned at Chaoyang, Beijing
China Committee for Culture Collection of Microorganisms's common micro-organisms center of North Star West Road, district 1 institute 3, preserving number is CGMCC
NO.12801。
Another object of the present invention is to provide a kind of anti-saline and alkaline microbial manure containing described strain, this fertilizer includes
Microbial bacterial agent, soil conditioner and trace element replenisher, wherein, microbial bacterial agent accounts for the 80% of microbial manure gross mass
~90%, soil conditioner accounts for the 7%~12% of microbial manure gross mass, and trace element replenisher account for the total matter of microbial manure
The 3%~7% of amount;Described microbial bacterial agent comes penicillium and bacillus subtilis containing ratio.
Preferably, microbial bacterial agent accounts for the 85% of microbial manure gross mass, and soil conditioner accounts for microbial total quality
10%, trace element replenisher account for the 5% of microbial manure gross mass.
Preferably, described microbial bacterial agent is by than carrying out penicillium and bacillus subtilis forms, and wherein, ratio carrys out penicillium sp mycoplasma
Amount ratio: bacillus subtilis mass ratio=2:1:1~2.
Preferably, described soil conditioner is aluminum sulfate, polyvinylidene alcohol, polyacrylamide.
Preferably, described trace element replenisher include zinc sulfate and boric acid.
It is highly preferred that the mass ratio of zinc sulfate and boric acid is: zinc sulfate: boric acid=3:2.
Preferably, described fertilizer is by accounting for the microbial bacterial agent of fertilizer gross mass 85%, the soil conditioner of 10% and 5%
Trace element forms, and wherein, microbial bacterial agent comprises ratio and comes penicillium and bacillus subtilis, and both mass ratioes are blueer or green than coming
Mycete: bacillus subtilis=1:1.5;Described soil conditioner is aluminum sulfate;Described trace element replenisher be zinc sulfate and
Boric acid, and the mass ratio of zinc sulfate and boric acid is: zinc sulfate: boric acid=3:2.
Preferably, dosage form be the viable count of granule or powder, granule and powder be 109cfu/g。
The above arbitrary fertilizer application in the repair process of salt-soda soil is also within protection scope of the present invention.
The beneficial effect that the present invention obtains:
1, the microbial manure of the present invention and soil conditioner scientific compatibility, it is possible to effectively reduce the pH value of saline-alkali soil, change
Kind soil physics environment;
2, the chemical pesticide such as the alternative thiram of the microbial manure of the present invention and metalaxyl, effectively suppression crop is vertical withered
Disease, especially rice seedling blight;
3, the microbial manure of the present invention can be with non-pulse family nitrogen-fixing bacteria scientific compatibility.Can fixed nitrogen, phosphorus decomposing, release potassium and trace unit
Element, can subtract to fertilize 20% 30%, and the not underproduction is not polluted, protected existing cultivated land resource.
Accompanying drawing explanation
Fig. 1 is the impact on the rice shoot of rice seedling blight of the microbial manure of the present invention.
(a, the growing state of rice seedling before suing and labouring;B, applies the microbial manure of admixture soil;C, seedling after applying fertilizer 3 days
The growing state of Seedling).
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be further described, but the present invention should not be limited by the examples.
Following example agents useful for same, material, method and instrument, without specified otherwise, be this area conventional reagent, material
Material, method and instrument, those skilled in the art all can be obtained by commercial channel.
The preparation of embodiment 1 microbial manure
Inventor selects ratio to come penicillium, bacillus subtilis, Bacillus licheniformis and molten phosphorus penicillium oxalicum
(Penicillium oxalium) is as the raw material bacterium of fertilizer microbial bacterial agent.And use aluminum sulfate as soil conditioner,
The mixture that zinc sulfate and boric acid obtain according to the mass ratio of 6:4 is as trace element replenisher.The microbial-bacterial fertilizer made
In microbial bacterial agent, the proportioning of each bacterium is as shown in table 1.
The composition of microbial inoculum in table 1 microbial-bacterial fertilizer
After preparing microbial bacterial agent according to the composition of table 1 and proportioning, then microbial bacterial agent is regulated with above-mentioned soil
Agent and trace element replenisher are according to microbial bacterial agent 85%, soil adjustment agent 10%, the mass ratio of trace element replenisher 5%
Granule or powder fertilizer is made after example mixing.
The improvement to soil pH of embodiment 2 microbial manure
Choose the alkaline land soil of the salt and alkali paddy rice Tanaka on affluence farm, Fuyu County, Heilongjiang Province, utilize embodiment 1 made
Standby microbial-bacterial fertilizer carries out microbial-bacterial fertilizer and soil pH is improved experiment.Choose and utilize the microbial inoculum 1-6 of embodiment 1 to prepare
Microbial manure, is respectively added to microbial inoculum 1-6 in soil according to the addition on 8 kilograms of every mu of ground, after 2 days, measures soil
Earth pH the initial pH with soil contrast, and result is as shown in table 2.
The impact on alkaline land soil pH of table 2 microbial bacterial agent
|
Microbial inoculum 1 |
Microbial inoculum 2 |
Microbial inoculum 3 |
Microbial inoculum 4 |
Microbial inoculum 5 |
Microbial inoculum 6 |
Before interpolation |
9.5 |
9.5 |
9.5 |
9.5 |
9.5 |
9.5 |
After interpolation |
9.4 |
8.5 |
9.3 |
9.2 |
8.4 |
8.3 |
As known from Table 2, ratio is being taken the penicillium microbial inoculum (microbial inoculum 2,5 and 6) with bacillus subtilis mixed preparing to soil
The improvement of earth pH is fairly obvious, and is only suitable for bacillus subtilis, or is coordinated with other phosphate solubilizing bacterias by bacillus subtilis and make
With the most limited to the improvement effect of soil pH.And from the point of view of microbial inoculum 2,5 and 6, higher to soil than carrying out penicillium usage amount
Improve effect the best.
The impact on rice yield of embodiment 3 microbial manure
In the present embodiment inventor select microbial inoculum 2 as the microbial inoculum of microbial manure, after preparing microbial-bacterial fertilizer, then profit
Field experiment is carried out, to test the impact on rice yield of this bacterial manure with this bacterial manure.Heilongjiang Province's matter delivered to by the fertilizer prepared
Amount supervisory detection academy carries out viable count detection, and wherein, in powder bacterial manure, every gram of fertilizer viable count reaches 6.24 hundred million, granule
In bacterial manure, in every gram of bacterial manure, viable count reaches 2.7 hundred million.Inventor after preparing microbial inoculum, takes Fuyu County to rich by this microbial inoculum
No. 0 salt-soda soil of pasture 11 team carries out rice cropping experiment.Experimental group is making according to per hectare 40Kg on the basis of normal fertilising
Consumption adds microbial manure, and matched group the most normally applies fertilizer.Meanwhile, inventor normally executes with 90%, 80%, 70% and 60%
Add microbial manure according to the usage amount of per hectare 40Kg on the basis of fertile amount and decrement matched group is set.After results, inventor
Being contrasted the yield data of experimental group and comparison, result is as shown in table 3.
The impact on rice yield of table 3 microbial manure
As known from Table 3, from the point of view of the time of infertility, identical kind, identical soil fertility, the crop of microbe microbial inoculum is raw
Long growth is preferably.Meanwhile, the crop anti-adversity ability using microbial-bacterial fertilizer is relatively strong, emerges neat, well developed root system, and capillary root system increases
Many, full seed, per mu yield significantly improves.The output condition of inventor's decrement matched group to reducing dose has been also carried out surveying
Fixed, result shows, the yield of the decrement matched group reducing fertilising 20% and 30% respectively reaches per mu yield 643Kg and 628Kg.This says
Bright, in the case of the dose reducing 20%-30%, rice yield is close with the matched group yield of normal dose, therefore,
The use of 20%~30% fertilizer can be reduced in the case of applying microbial-bacterial fertilizer.
The improvement to rice seedling blight of embodiment 4 microbial manure
In the development process of bacterial manure, inventor chances on this bacterial manure and has significant inhibitory action to rice seedling blight.
The damping-off evil occurred during seedling cultivation of rice, microbial inoculum is mixed soil and is spread and return to Seedling Tanaka and give treatment to by inventor, and usage amount is every
Square meter 100g bacterial manure, (see accompanying drawing 1, in figure, the place of arrow indication is to lack withered rice seedlings gradually polishing after three days as a result
The place of damping-off occurs).
Although the present invention is open the most as above with preferred embodiment, but it is not limited to the present invention, any is familiar with this
The people of technology, without departing from the spirit and scope of the present invention, can do various change and modification, the therefore protection of the present invention
Scope should be with being as the criterion that claims are defined.