CN106282035A - A kind of anti-saline and alkaline microbial manure and application - Google Patents

A kind of anti-saline and alkaline microbial manure and application Download PDF

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Publication number
CN106282035A
CN106282035A CN201610790475.4A CN201610790475A CN106282035A CN 106282035 A CN106282035 A CN 106282035A CN 201610790475 A CN201610790475 A CN 201610790475A CN 106282035 A CN106282035 A CN 106282035A
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microbial
microbial manure
soil
bacterial agent
fertilizer
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CN106282035B (en
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王晓辉
李莉莉
张鼎智
王玉坤
王东凯
吴琼
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Dong Xiaotang
Li Wudi
Sheng Jianwei
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Harbin Futai To Ze Biotechnology Co Ltd
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/145Fungal isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • C12R2001/80Penicillium
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • C05D9/02Other inorganic fertilisers containing trace elements
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2109/00MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation

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Abstract

The invention discloses a kind of anti-saline and alkaline microbial manure and application, belong to agricultural microbial manure technical field.Anti-saline and alkaline microbial manure provided by the present invention includes microbial bacterial agent, soil conditioner and trace element.Wherein, microbial bacterial agent accounts for the 80%~90% of microbial manure gross mass, and soil conditioner accounts for the 7%~12% of microbial manure gross mass, and trace element accounts for the 3%~7% of microbial manure gross mass;Described microbial bacterial agent comes penicillium and bacillus subtilis containing ratio.Microbial manure provided by the present invention and soil conditioner scientific compatibility, it is possible to effectively reduce the pH value of saline-alkali soil, improve soil physics environment, the most alternative chemical pesticide, effectively suppress the damping-off of crop.Further, the microbial manure being somebody's turn to do can be with non-pulse family nitrogen-fixing bacteria scientific compatibility.Can fixed nitrogen, phosphorus decomposing, release potassium and trace element, can subtract to fertilize 20% 30%, the not underproduction, do not pollute, protect existing cultivated land resource.

Description

A kind of anti-saline and alkaline microbial manure and application
Technical field
The present invention relates to a kind of anti-saline and alkaline microbial manure and application, belong to agricultural microbial manure technical field.
Background technology
Salt-soda soil refers to that salinity contained in soil has influence on the salt aggregation soil of plant growth.According to associating state religion Section's literary composition tissue and the incomplete statistics of the World Food Programme, global salt-soda soil area alreadys more than 9.5 hundred million hectares, wherein, China has just reached 99,130,000 hectares.China's alkaline earth and the formation of alkali-affected soil, the accumulation of the carbonate in major part and soil Relevant, salinization and alkalization is the highest, and serious salt affected soil regional plant is little to existence.
Although currently available technology discloses some resist saline and alkaline fertilizer, but during these chemical fertilizer, although Effect is obvious in a short time, but the chemical fertilizer fertile phase itself is short, loses efficacy fast, and fertility persistency is poor, simultaneously can be original to ecological environment The most fragile alkaline land soil produces secondary chemical contamination, and, consumption is big, easily causes soil compaction.
Summary of the invention
For solving above-mentioned technical problem, the invention provides a kind of anti-saline and alkaline microbial manure, the technical scheme taked As follows:
It is an object of the invention to provide a kind of strain for preparing anti-saline and alkaline microbial manure, it is characterised in that institute State strain and carry out penicillium (Penicillium bilaiae) for ratio, on July 20th, 2016, be preserved in and be positioned at Chaoyang, Beijing China Committee for Culture Collection of Microorganisms's common micro-organisms center of North Star West Road, district 1 institute 3, preserving number is CGMCC NO.12801。
Another object of the present invention is to provide a kind of anti-saline and alkaline microbial manure containing described strain, this fertilizer includes Microbial bacterial agent, soil conditioner and trace element replenisher, wherein, microbial bacterial agent accounts for the 80% of microbial manure gross mass ~90%, soil conditioner accounts for the 7%~12% of microbial manure gross mass, and trace element replenisher account for the total matter of microbial manure The 3%~7% of amount;Described microbial bacterial agent comes penicillium and bacillus subtilis containing ratio.
Preferably, microbial bacterial agent accounts for the 85% of microbial manure gross mass, and soil conditioner accounts for microbial total quality 10%, trace element replenisher account for the 5% of microbial manure gross mass.
Preferably, described microbial bacterial agent is by than carrying out penicillium and bacillus subtilis forms, and wherein, ratio carrys out penicillium sp mycoplasma Amount ratio: bacillus subtilis mass ratio=2:1:1~2.
Preferably, described soil conditioner is aluminum sulfate, polyvinylidene alcohol, polyacrylamide.
Preferably, described trace element replenisher include zinc sulfate and boric acid.
It is highly preferred that the mass ratio of zinc sulfate and boric acid is: zinc sulfate: boric acid=3:2.
Preferably, described fertilizer is by accounting for the microbial bacterial agent of fertilizer gross mass 85%, the soil conditioner of 10% and 5% Trace element forms, and wherein, microbial bacterial agent comprises ratio and comes penicillium and bacillus subtilis, and both mass ratioes are blueer or green than coming Mycete: bacillus subtilis=1:1.5;Described soil conditioner is aluminum sulfate;Described trace element replenisher be zinc sulfate and Boric acid, and the mass ratio of zinc sulfate and boric acid is: zinc sulfate: boric acid=3:2.
Preferably, dosage form be the viable count of granule or powder, granule and powder be 109cfu/g。
The above arbitrary fertilizer application in the repair process of salt-soda soil is also within protection scope of the present invention.
The beneficial effect that the present invention obtains:
1, the microbial manure of the present invention and soil conditioner scientific compatibility, it is possible to effectively reduce the pH value of saline-alkali soil, change Kind soil physics environment;
2, the chemical pesticide such as the alternative thiram of the microbial manure of the present invention and metalaxyl, effectively suppression crop is vertical withered Disease, especially rice seedling blight;
3, the microbial manure of the present invention can be with non-pulse family nitrogen-fixing bacteria scientific compatibility.Can fixed nitrogen, phosphorus decomposing, release potassium and trace unit Element, can subtract to fertilize 20% 30%, and the not underproduction is not polluted, protected existing cultivated land resource.
Accompanying drawing explanation
Fig. 1 is the impact on the rice shoot of rice seedling blight of the microbial manure of the present invention.
(a, the growing state of rice seedling before suing and labouring;B, applies the microbial manure of admixture soil;C, seedling after applying fertilizer 3 days The growing state of Seedling).
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be further described, but the present invention should not be limited by the examples.
Following example agents useful for same, material, method and instrument, without specified otherwise, be this area conventional reagent, material Material, method and instrument, those skilled in the art all can be obtained by commercial channel.
The preparation of embodiment 1 microbial manure
Inventor selects ratio to come penicillium, bacillus subtilis, Bacillus licheniformis and molten phosphorus penicillium oxalicum (Penicillium oxalium) is as the raw material bacterium of fertilizer microbial bacterial agent.And use aluminum sulfate as soil conditioner, The mixture that zinc sulfate and boric acid obtain according to the mass ratio of 6:4 is as trace element replenisher.The microbial-bacterial fertilizer made In microbial bacterial agent, the proportioning of each bacterium is as shown in table 1.
The composition of microbial inoculum in table 1 microbial-bacterial fertilizer
After preparing microbial bacterial agent according to the composition of table 1 and proportioning, then microbial bacterial agent is regulated with above-mentioned soil Agent and trace element replenisher are according to microbial bacterial agent 85%, soil adjustment agent 10%, the mass ratio of trace element replenisher 5% Granule or powder fertilizer is made after example mixing.
The improvement to soil pH of embodiment 2 microbial manure
Choose the alkaline land soil of the salt and alkali paddy rice Tanaka on affluence farm, Fuyu County, Heilongjiang Province, utilize embodiment 1 made Standby microbial-bacterial fertilizer carries out microbial-bacterial fertilizer and soil pH is improved experiment.Choose and utilize the microbial inoculum 1-6 of embodiment 1 to prepare Microbial manure, is respectively added to microbial inoculum 1-6 in soil according to the addition on 8 kilograms of every mu of ground, after 2 days, measures soil Earth pH the initial pH with soil contrast, and result is as shown in table 2.
The impact on alkaline land soil pH of table 2 microbial bacterial agent
Microbial inoculum 1 Microbial inoculum 2 Microbial inoculum 3 Microbial inoculum 4 Microbial inoculum 5 Microbial inoculum 6
Before interpolation 9.5 9.5 9.5 9.5 9.5 9.5
After interpolation 9.4 8.5 9.3 9.2 8.4 8.3
As known from Table 2, ratio is being taken the penicillium microbial inoculum (microbial inoculum 2,5 and 6) with bacillus subtilis mixed preparing to soil The improvement of earth pH is fairly obvious, and is only suitable for bacillus subtilis, or is coordinated with other phosphate solubilizing bacterias by bacillus subtilis and make With the most limited to the improvement effect of soil pH.And from the point of view of microbial inoculum 2,5 and 6, higher to soil than carrying out penicillium usage amount Improve effect the best.
The impact on rice yield of embodiment 3 microbial manure
In the present embodiment inventor select microbial inoculum 2 as the microbial inoculum of microbial manure, after preparing microbial-bacterial fertilizer, then profit Field experiment is carried out, to test the impact on rice yield of this bacterial manure with this bacterial manure.Heilongjiang Province's matter delivered to by the fertilizer prepared Amount supervisory detection academy carries out viable count detection, and wherein, in powder bacterial manure, every gram of fertilizer viable count reaches 6.24 hundred million, granule In bacterial manure, in every gram of bacterial manure, viable count reaches 2.7 hundred million.Inventor after preparing microbial inoculum, takes Fuyu County to rich by this microbial inoculum No. 0 salt-soda soil of pasture 11 team carries out rice cropping experiment.Experimental group is making according to per hectare 40Kg on the basis of normal fertilising Consumption adds microbial manure, and matched group the most normally applies fertilizer.Meanwhile, inventor normally executes with 90%, 80%, 70% and 60% Add microbial manure according to the usage amount of per hectare 40Kg on the basis of fertile amount and decrement matched group is set.After results, inventor Being contrasted the yield data of experimental group and comparison, result is as shown in table 3.
The impact on rice yield of table 3 microbial manure
As known from Table 3, from the point of view of the time of infertility, identical kind, identical soil fertility, the crop of microbe microbial inoculum is raw Long growth is preferably.Meanwhile, the crop anti-adversity ability using microbial-bacterial fertilizer is relatively strong, emerges neat, well developed root system, and capillary root system increases Many, full seed, per mu yield significantly improves.The output condition of inventor's decrement matched group to reducing dose has been also carried out surveying Fixed, result shows, the yield of the decrement matched group reducing fertilising 20% and 30% respectively reaches per mu yield 643Kg and 628Kg.This says Bright, in the case of the dose reducing 20%-30%, rice yield is close with the matched group yield of normal dose, therefore, The use of 20%~30% fertilizer can be reduced in the case of applying microbial-bacterial fertilizer.
The improvement to rice seedling blight of embodiment 4 microbial manure
In the development process of bacterial manure, inventor chances on this bacterial manure and has significant inhibitory action to rice seedling blight. The damping-off evil occurred during seedling cultivation of rice, microbial inoculum is mixed soil and is spread and return to Seedling Tanaka and give treatment to by inventor, and usage amount is every Square meter 100g bacterial manure, (see accompanying drawing 1, in figure, the place of arrow indication is to lack withered rice seedlings gradually polishing after three days as a result The place of damping-off occurs).
Although the present invention is open the most as above with preferred embodiment, but it is not limited to the present invention, any is familiar with this The people of technology, without departing from the spirit and scope of the present invention, can do various change and modification, the therefore protection of the present invention Scope should be with being as the criterion that claims are defined.

Claims (10)

1. the strain being used for preparing anti-saline and alkaline microbial manure, it is characterised in that described strain carrys out penicillium for ratio (Penicillium bilaiae), on July 20th, 2016, is preserved in and is positioned at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCC NO.12801.
2. the anti-saline and alkaline microbial manure containing strain described in claim 1, it is characterised in that include microbial bacterial agent, Soil conditioner and trace element replenisher, wherein, microbial bacterial agent accounts for the 80%~90% of microbial manure gross mass, soil Regulator accounts for the 7%~12% of microbial manure gross mass, trace element replenisher account for microbial manure gross mass 3%~ 7%;Described microbial bacterial agent comes penicillium and bacillus subtilis containing ratio.
3. the fertilizer described in claim 2, it is characterised in that microbial bacterial agent accounts for the 85% of microbial manure gross mass, soil Regulator accounts for the 10% of microbial total quality, and trace element replenisher account for the 5% of microbial manure gross mass.
4. the fertilizer described in claim 2, it is characterised in that described microbial bacterial agent is come penicillium and bacillus subtilis by ratio Composition, wherein, ratio carrys out penicillium mass ratio: bacillus subtilis mass ratio=2:1~1:2.
5. the fertilizer described in claim 2, it is characterised in that described soil conditioner is aluminum sulfate, polyvinyl alcohol, polyethylene acyl Amine.
6. the fertilizer described in claim 2, it is characterised in that described trace element replenisher include zinc sulfate and boric acid.
7. the fertilizer described in claim 6, it is characterised in that the mass ratio of zinc sulfate and boric acid is: zinc sulfate: boric acid=3:2.
8. the fertilizer described in claim 2, it is characterised in that by accounting for the microbial bacterial agent of fertilizer gross mass 85%, the soil of 10% Earth regulator and the trace element composition of 5%, wherein, microbial bacterial agent comprises ratio and comes penicillium and bacillus subtilis, both Mass ratio carry out penicillium for ratio: bacillus subtilis=1:1.5;Described soil conditioner is aluminum sulfate;Described trace element Supplement are zinc sulfate and boric acid, and the mass ratio of zinc sulfate and boric acid is: zinc sulfate: boric acid=3:2.
9. the fertilizer described in claim 2, it is characterised in that dosage form is granule or powder.
10. arbitrary fertilizer application in the repair process of salt-soda soil described in claim 2-9.
CN201610790475.4A 2016-08-31 2016-08-31 A kind of salt resistance alkali microbial manure and application Active CN106282035B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113354477A (en) * 2021-07-28 2021-09-07 黑龙江神飞生物科技股份有限公司 Special compound fertilizer rich in microbial agent for soybeans and preparation method thereof
CN113545263A (en) * 2021-07-28 2021-10-26 青岛万慧源环保科技有限公司 Method for increasing yield of rice in saline-alkali soil

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CN105072915A (en) * 2013-03-20 2015-11-18 巴斯夫公司 Synergistic compositions comprising a bacillus subtilis strain and a biopesticide
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CN105072915A (en) * 2013-03-20 2015-11-18 巴斯夫公司 Synergistic compositions comprising a bacillus subtilis strain and a biopesticide
CN104909870A (en) * 2015-05-11 2015-09-16 张文 Saline-alkaline and hardened soil improvement and remediation agent
CN105348009A (en) * 2015-12-02 2016-02-24 合肥四方磷复肥有限责任公司 Compound amino acid microbial fertilizer for saline-alkali land and preparation method of fertilizer

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113354477A (en) * 2021-07-28 2021-09-07 黑龙江神飞生物科技股份有限公司 Special compound fertilizer rich in microbial agent for soybeans and preparation method thereof
CN113545263A (en) * 2021-07-28 2021-10-26 青岛万慧源环保科技有限公司 Method for increasing yield of rice in saline-alkali soil

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