CN106282013B - A kind of device convenient for microorganism ultraviolet mutagenesis - Google Patents
A kind of device convenient for microorganism ultraviolet mutagenesis Download PDFInfo
- Publication number
- CN106282013B CN106282013B CN201610815288.7A CN201610815288A CN106282013B CN 106282013 B CN106282013 B CN 106282013B CN 201610815288 A CN201610815288 A CN 201610815288A CN 106282013 B CN106282013 B CN 106282013B
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- CN
- China
- Prior art keywords
- resin
- kickboard
- bacterium solution
- beaker
- microorganism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Electromagnetism (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention discloses a kind of devices convenient for microorganism ultraviolet mutagenesis, pass through a built-in bacterium solution of uncovered beaker, one hollow resin kickboard can float over bacterium solution surface, the glass plate of resin kickboard upper surface can be uniformly distributed microbial cell and be irradiated convenient for ultraviolet light, stop irradiation of the ultraviolet light to bacterium solution simultaneously, iron cake is inlayed in resin kickboard, bacterium solution can be immersed under the attraction effect of magnet in magnet pad, bring mutant cell into bacterium solution.Resin kickboard can high pressure steam sterilization, beaker opening can with plastics sealer seal prevent living contaminants, convenient for sterile working, but convenient for ultraviolet light penetrate.The present invention improves bacterial strain bioactivity, improved strain character, creates new gene, new enzyme, novel protein, microorganism orthogenesis, directed enzyme evolution etc., screening is with new traits, new gene, the bacterial strain of new enzyme for microorganism ultraviolet mutagenesis.
Description
Technical field
The present invention relates to microbial apparatus fields, especially a kind of device convenient for microorganism ultraviolet mutagenesis.
Background technology
Microorganism spontaneous mutation frequency is too low, and spontaneous evolution is too slow, and ultraviolet mutagenesis can increase substantially microbial mutation
Frequency and evolutionary rate.It is a kind of random mutation caused by carrying out mutagenesis to microorganism using ultraviolet light, mutation effect majority is
Lethal or harmful.As long as but a mutation is beneficial, so that it may utilize the fireballing feature of microbial growth
The beneficial bacterial strain of mutation is expanded, so as to achieve the purpose that screen beneficial mutation.Existing equipment be difficult to integrated ultraviolet light irradiation,
The technologies such as mutagenesis, amplification, enrichment, reset.
The content of the invention
The purpose of the present invention is：In order to overcome the above-mentioned deficiency of the prior art, the present invention provides a kind of purple convenient for microorganism
The device of outside line mutagenesis, for microorganism ultraviolet mutagenesis, improve bacterial strain bioactivity, improved strain character, create new gene,
New enzyme, novel protein, microorganism orthogenesis, directed enzyme evolution etc..
The technical solution adopted by the present invention to solve the technical problems is：A kind of dress convenient for microorganism ultraviolet mutagenesis
It puts, including beaker (1), resin kickboard (5) and magnet pad (9), beaker (1) is cylinder, has bottom, opening, by glass construction, tree
Fat kickboard (5) is round pie, and diameter is 10 millimeters shorter than beaker (1) internal diameter, and resin kickboard (5) lower inner part inlays iron cake (7),
Upper outside surfaces mosaic glass plectane (4), iron cake (7) are irony, are inside resin kickboard (5) hollow (6), resin kickboard
(5) rest part is constructed by resin, 0.8-0.95 grams/cc of resin kickboard (5) global density, beaker (1) dress liquid training
It supports base and is inoculated with Spawn incubation into bacterium solution (3), resin kickboard (5) floats on liquid level (2), and magnet pad (9) is round pie, and surface is skin
Matter, the internal embedded magnet heart (8), magnetic core (8) be magnet matter, round pie.
The beneficial effects of the invention are as follows：Resin kickboard (5) can float on bacterium solution (3) liquid level (2) in beaker (1), so as to block
Ultraviolet light irradiates bacterium solution (3), and resin kickboard (5) density is less than water, and built-in iron cake (7) attracts resin convenient for magnetic core (8)
Kickboard (5) is immersed in liquid, and resin kickboard (5) upper surface is the nature of glass, can not only stop ultraviolet light, but also bacterium cell can be made uniform
Surface is distributed in, convenient for ultraviolet radiation mutagenesis, device is convenient for sterile working, and resin kickboard (5) material can high pressure steaming for resin
Vapour sterilizes, and adsorbable resin kickboard (5) the submergence bacterium solution (3) of magnet built in magnet pad (9), housing leather packing prevents from breaking up beaker (1),
The present invention is convenient for microorganism ultraviolet mutagenesis, and screening is with new traits, new gene, the bacterial strain of new enzyme.
Description of the drawings
The present invention is further described with reference to the accompanying drawings and examples.
Fig. 1 is the structure diagram of one embodiment of the invention.
1. beaker in figure, 2. liquid levels, 3. bacterium solutions, 4. glass plectanes, 5. resin kickboards, 6. is hollow, 7. iron cakes, 8. magnet
The heart, 9. magnet pads.
Specific embodiment
In Fig. 1, beaker (1) for it is cylindrical, have bottom, opening, a nature of glass, resin kickboard (5), round pie, external resin matter,
Inside is hollow (6), and iron cake (7), hollow another side external surface mosaic glass circle of (6) inner face are inlayed in hollow (6) inner face one side
Plate (4), 0.8-0.95 grams/cc of resin kickboard (5) global density, beaker (1) dress fluid nutrient medium are trained bacterium solution
(3), resin kickboard (5) floats on liquid level (2), magnet pad (9), round pie, the core embedded magnet heart (8).
The course of work of diagram embodiment of the present invention is：A. strain is inoculated into bacterium solution (3), is sealed with plastics sealer
Beaker (1) is open, shaking table culture to muddiness；B. beaker (1) is rested on magnet pad (9), resin kickboard (5) is attracted to soak by magnet
Not in bacterium solution (3), due to the immersional wetting of glass, a large amount of bacterium cells of adsorption；C. beaker (1) is moved away, magnet pad (9)
Attraction disappears, on resin kickboard (5) emersion liquid level (2)；D. beaker (1) is placed under ultraviolet lamp and irradiates mutagenesis；E. glass is justified
Under ultraviolet light irradiation gene mutation occurs for the bacterial cell on plate (4) surface；F. beaker (1) is rested on magnet pad (9), resin
Kickboard (5) is immersed in by magnet attraction in bacterium solution (3)；G. mutant cell enters in bacterium solution (3).
Claims (1)
1. a kind of device convenient for microorganism ultraviolet mutagenesis, including beaker (1), resin kickboard (5) and magnet pad (9), beaker
(1) it is cylinder, has bottom, opening, by glass construction, resin kickboard (5) is round pie, and diameter shorter than beaker (1) internal diameter 10 is in the least
Rice, resin kickboard (5) lower inner part inlay iron cake (7), upper outside surfaces mosaic glass plectane (4), and iron cake (7) is iron
Matter, internal resin kickboard (5) is hollow (6), and resin kickboard (5) rest part is constructed by resin, resin kickboard (5) global density
0.8-0.95 grams/cc, beaker (1) dress fluid nutrient medium inoculating strain is trained bacterium solution (3), and resin kickboard (5) floats on
Liquid level (2), magnet pad (9) be round pie, surface is cortex, the internal embedded magnet heart (8), magnetic core (8) be magnet matter, cake
Shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610815288.7A CN106282013B (en) | 2016-09-09 | 2016-09-09 | A kind of device convenient for microorganism ultraviolet mutagenesis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610815288.7A CN106282013B (en) | 2016-09-09 | 2016-09-09 | A kind of device convenient for microorganism ultraviolet mutagenesis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106282013A CN106282013A (en) | 2017-01-04 |
| CN106282013B true CN106282013B (en) | 2018-06-01 |
Family
ID=57709958
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610815288.7A Expired - Fee Related CN106282013B (en) | 2016-09-09 | 2016-09-09 | A kind of device convenient for microorganism ultraviolet mutagenesis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106282013B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112710528B (en) * | 2020-12-17 | 2023-02-21 | 深圳市职业病防治院 | Sister chromatid interchange differential staining auxiliary device and method |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4498785A (en) * | 1982-06-09 | 1985-02-12 | Techne Corporation | Floating magnetic stirrer for culture medium |
| KR20180004300A (en) * | 2013-07-19 | 2018-01-10 | 생-고뱅 퍼포먼스 플라스틱스 코포레이션 | Reciprocating fluid agitator |
| CN205528821U (en) * | 2015-12-31 | 2016-08-31 | 天津泰创生物科技有限公司 | Ultra -violet analysis appearance |
-
2016
- 2016-09-09 CN CN201610815288.7A patent/CN106282013B/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN106282013A (en) | 2017-01-04 |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180601 Termination date: 20200909 |
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| CF01 | Termination of patent right due to non-payment of annual fee |