CN106281978A - A kind of combination type froth breaking oar and the miniature organism reactor of use combination type froth breaking oar - Google Patents
A kind of combination type froth breaking oar and the miniature organism reactor of use combination type froth breaking oar Download PDFInfo
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- CN106281978A CN106281978A CN201610643053.4A CN201610643053A CN106281978A CN 106281978 A CN106281978 A CN 106281978A CN 201610643053 A CN201610643053 A CN 201610643053A CN 106281978 A CN106281978 A CN 106281978A
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- ring
- froth breaking
- combination type
- breaking oar
- shaft
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
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- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
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- General Health & Medical Sciences (AREA)
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- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Mixers Of The Rotary Stirring Type (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of combination type froth breaking oar and use the miniature organism reactor of combination type froth breaking oar, this combination type froth breaking oar includes that blade, back-up ring, a pair blade are horizontally set on the outer ring of back-up ring, and blade place passes straight through the geometric center of back-up ring;Described miniature organism reactor includes tank body, shaft, cover, mechanical seal and stirring paddle;Shaft, through the centre bore of cover, is arranged in the inner chamber of tank body;Mechanical sealing linkage ring, stirring paddle are arranged on shaft;Shaft is additionally provided with combination type froth breaking oar.The combination type froth breaking oar of present invention offer and the miniature organism reactor of use combination type froth breaking oar, its compact conformation, rationally distributed, froth breaking oar uses fabricated structure with the back-up ring of mechanical sealing linkage ring, on the basis of ensureing defoaming function, it is effectively increased the coefficient of miniature organism reactor, simplifies tank inner structure simultaneously, convenient to operate, clean, reduce dead angle risk, more preferably meet the use requirement of microreactor.
Description
Technical field
The present invention relates to microreactor technical field, a kind of combination type froth breaking oar and use combination type froth breaking oar
Miniature organism reactor.
Background technology
In recent years, designative species has played increasing effect in national economy, have received more multimedia and
The concern of common people.The technology source of fermentable pharmacy is the strain of microorganism, and a good microorganism fungus kind is necessarily
The quality and quantity of the medicine that fermentable produces is determined in degree.And the High Throughput Screening Assay of strain is its performance improvement
One of important step.
At present, High Throughput Screening Assay obtains because its screening efficiency is high paying attention to and development day by day, and high flux screening needs
To complete by miniature organism reactor.
Froth breaking oar is the vitals of bioreactor, and its effect is to smash the ventilation of reactor endogenous cause of ill and the bubble of stirring formation
Foam, prevents foam from taking out of in reactor with aerofluxus, causes reactor to escape liquid.
Conventional bioreactor construction schematic diagram, as shown in Figure 1.Conventional bioreactor includes tank body, cover, stirs
Mix axle, mechanical sealing linkage ring, mechanical sealing static ring, stirring paddle, conventional back-up ring, the conventional froth breaking oar that is made up of blade and wheel hub.
Be positioned at the mechanical sealing linkage ring of tank interior, back-up ring, froth breaking oar are Split type structure, and back-up ring is arranged on for compression and stirs
Mix the mechanical sealing linkage ring on axle, cause mechanical sealing linkage ring and the stationary ring being positioned at cover locating slot mutually compresses, it is ensured that be anti-
Answer in device the sealing of gas to form tank pressure;Froth breaking oar is usually mounted on the shaft between liquid level and cover, according to liquid level
Height can carry out high high adjustment with foam situation, with control foam position height.
Owing to microreactor axial dimension requires less, as used traditional structure, the position of froth breaking oar will reduce, shadow
Ring the coefficient of microreactor, in the confined space, add the difficulty of operation and maintenance simultaneously.
Summary of the invention
It is an object of the invention to provide a kind of combination type froth breaking oar and use the miniature organism reactor of combination type froth breaking oar,
On the basis of not affecting defoaming function, effectively reduce the axial space of miniature organism reactor, it is simple to clean and safeguard, letter
Internal structure and the operation requirement of the microreactor for high flux screening are changed.
The technical scheme that the present invention provides is as follows:
A kind of combination type froth breaking oar, comprising: a pair blade and back-up ring, the pair of blade is horizontally set on outside back-up ring
Enclose, and the pair of blade place passes straight through the geometric center of back-up ring.
Concrete, a pair blade is arranged on the outer ring of back-up ring, its position level of relative, blade place pass straight through gear
The geometric center of circle.
It is further preferred that the bottom of described blade is provided with zigzag structure, or flat impeller structure, or orthogonal circle
Bar and the combinative structure of thin bar, to ensure good defoaming effect.
Concrete, the zigzag that blade bottom is used according to requirements, flat circular bar shape leaf, horizontal or round bar add
Bubble contact on liquid level in the form structures, with tank body such as dress montant, it is easier to poke bubble, thus in destroying tank body on liquid level
Bubble, it is ensured that good defoaming effect.
It is further preferred that the bottom of described blade flushes with the bottom of back-up ring.
Concrete, that the bottom of blade flushes with the bottom of back-up ring design, is effectively ensured combination type froth breaking oar processing technology
Uniformity, thus ensure the concordance of froth breaking paddle structure, to avoid multi-channel miniature reactor based on parallel test principle
Internal components physical dimension is inconsistent each other, and affects the test data of high flux screening.
Present invention also offers a kind of miniature organism reactor, it include tank body, shaft, cover, mechanical sealing linkage ring,
Mechanical sealing static ring and stirring paddle;Described mechanical sealing static ring is fixed on below cover;Shaft passes the centre bore of cover,
It is arranged in the inner chamber of tank body;Described mechanical sealing linkage ring, stirring paddle are arranged on shaft;Shaft is additionally provided with as
Aforesaid combination type froth breaking oar.
It is further preferred that described combination type froth breaking oar is arranged on the bottom of described mechanical sealing linkage ring, wherein, described group
Described mechanical sealing linkage ring is pressed to realize axial limiting in the top of the back-up ring on box-like froth breaking oar.
It is further preferred that the position that described combination type froth breaking oar is on described shaft is adjustable, with regulation to described machine
The compression degree of tool moving sealing ring.
It is further preferred that after described combination type froth breaking oar position on described shaft determines, pass through clamp screw
Described combination type froth breaking oar is fixed on described shaft by nail.
It is further preferred that after described combination type froth breaking oar position on described shaft determines, by wearing pin
Described back-up ring is fixed on described shaft by post, to be fixed on described shaft by described combination type froth breaking oar, fixes
Crush to described mechanical sealing linkage ring.
A kind of combination type froth breaking oar provided by the present invention and the miniature organism reactor using combination type froth breaking oar, energy
Enough bring at least one beneficial effect following:
(1) froth breaking oar employing fabricated structure, compact conformation, rationally distributed, it is ensured that the froth breaking of miniature organism reactor
Function.
(2) use combination type froth breaking oar, can effectively reduce the axial space of miniature organism reactor, meet microreactor
Use with operation requirement.The stirring system of standard biologic reactor, is by the back-up ring of mechanical sealing linkage ring with froth breaking oar each
Being independently arranged, and the tank body physical dimension of miniature organism reactor is less, especially tank inner height is inadequate, arrange two independent
Structure (back-up ring and froth breaking oar) just makes froth breaking oar position too low, reduces the coefficient of microreactor, simultaneously in the confined space
Inside add the difficulty of operation and maintenance.In the present invention, the back-up ring being used for compressing mechanical sealing linkage ring is integrated into froth breaking oar
Integrally, mechanical sealing linkage ring is both compressed by the back-up ring in combination type froth breaking oar, and back-up ring makees again the wheel hub use of froth breaking oar simultaneously;And
And blade also acts as froth breaking effect.Such structure just can both play the position-limiting action to mechanical sealing linkage ring, keeps away again simultaneously
The position exempting from froth breaking oar is too low, can effectively pulverize foam.Further, the two mutually integrated structure this most effectively reduces
The axial space requirement of miniature organism reactor.
Accompanying drawing explanation
Below by the way of the most understandable, accompanying drawings preferred implementation, to a kind of combination type froth breaking oar and
The above-mentioned characteristic, technical characteristic, advantage and the implementation thereof that use the miniature organism reactor of combination type froth breaking oar are entered one
Step explanation.
Fig. 1 is traditional bioreactor construction schematic diagram;
Fig. 2 is the structural representation of combination type a kind of embodiment of froth breaking oar of the present invention;
Fig. 3 is the structural representation that the present invention uses a kind of embodiment of miniature organism reactor of combination type froth breaking oar.
Drawing reference numeral illustrates:
1. back-up ring;2. blade;
3. shaft;4. tank body;5. stirring paddle;6. cover;7. mechanical sealing linkage ring;
8. tank body;9. cover;10. shaft;11. mechanical sealing linkage rings;12. stirring paddles;13. tradition back-up rings;14. tradition
Froth breaking oar.
Detailed description of the invention
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will comparison accompanying drawing explanation
The detailed description of the invention of the present invention.It should be evident that the accompanying drawing in describing below is only some embodiments of the present invention, for
From the point of view of those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to obtain other according to these accompanying drawings
Accompanying drawing, and obtain other embodiment.
For making simplified form, only schematically show part related to the present invention in each figure, they do not represent
It is as the practical structures of product.It addition, so that simplified form readily appreciates, some figure has identical structure or function
Parts, only symbolically depict one of them, or have only marked one of them.In this article, " one " not only represents
" only this ", it is also possible to represent the situation of " more than one ".
Fig. 1 is traditional bioreactor construction schematic diagram, and traditional bioreactor includes tank body 8, cover 9, stirring
Axle 10, mechanical sealing linkage ring 11, stirring paddle 12, tradition back-up ring 13, tradition froth breaking oar 14.
Be positioned at the traditional back-up ring 13 within tank body 8, tradition froth breaking oar 14 is Split type structure, and tradition back-up ring 13 sets for compression
Putting the mechanical sealing linkage ring 11 on shaft 10, cause mechanical sealing linkage ring 11 with the 9 stationary ring locating slots that are positioned at cover is quiet
Ring mutually compresses, it is ensured that in reactor, the sealing of gas forms tank pressure;Froth breaking oar is used for smashing the ventilation of tank endogenous cause of ill and is formed with stirring
Foam, prevent foam from causing in taking tank out of with aerofluxus and escape liquid, tradition froth breaking oar 14 be usually mounted between liquid level and cover 9
On shaft 10, high high adjustment can be carried out according to the height of liquid level with foam situation, to control foam position height.Back-up ring with disappear
Bubble oar relies on holding screw and laterallys secure in shaft from the hub positions of the two.And in microreactor, due to tank
Body physical dimension is little, and especially tank inner height is inadequate, arranges two wheel hubs and makes froth breaking oar position too low, and due to minisize reaction
Device axial dimension requires less, uses above-mentioned traditional structure, can reduce again the stocking volume of reactor, increase and clean and operation
Difficulty.
In the embodiment one of combination type froth breaking oar of the present invention, it includes a pair blade, back-up ring, wherein, a pair blade water
Put down the outer ring being arranged on back-up ring, and blade place passes straight through the geometric center of back-up ring, to realize symmetrical structure, thus ensures
The even results of froth breaking.By the structure of the present embodiment, it is possible to by the mechanical sealing linkage ring back-up ring in traditional structure and froth breaking oar
Unite two into one, effectively reduce time combination type froth breaking oar is applied in miniature organism reactor, to axial installing space size
Requirement, simplify the tank inner structure of reactor, it is simple to operate, clean, reduce dead angle risk simultaneously.
Preferably, on the basis of embodiment one, embodiments of the invention two are provided with zigzag knot in the bottom of blade
Structure, or flat impeller structure, or the combinative structure of orthogonal round bar and thin bar, to ensure good defoaming effect.
Preferably, on the basis of embodiment one, the bottom of the blade in embodiments of the invention three and the bottom of back-up ring
Flush, thus the uniformity of combination type froth breaking oar processing technology is effectively ensured, thus ensure the concordance of froth breaking paddle structure, to keep away
Exempt from multi-channel miniature reactor internal components physical dimension each other based on parallel test principle inconsistent, and affect high pass
The test data of amount screening.
In a concrete preferred embodiment of combination type froth breaking oar of the present invention, with reference to Fig. 2, combination type froth breaking oar bag
Including: blade 2, back-up ring 1, a pair blade 2 is horizontally set on the outer ring of back-up ring 1, and blade 2 place passes straight through in the geometry of back-up ring 1
The heart.The bottom of blade 2 is provided with zigzag structure, and the bottom of blade 2 flushes with the bottom of back-up ring 1.The combination type of the present invention
Froth breaking oar, on the premise of ensureing mixing effect, by back-up ring and the fabricated structure of blade, effectively reduces axial installation empty
Between size, thus when the combination type froth breaking oar of the present invention is applied in miniature organism reactor, by increasing capacitance it is possible to increase microreactor
Stocking volume, simplify the structure in reactor tank body, it is simple to operate, clean, reduce dead angle risk.
The invention also discloses a kind of miniature organism reactor.The miniature organism reactor occurred in the world at present is in structure
On generally include three classes: orifice-plate type microreactor (microtiter plate, MTP), based on existing shaking flask or fermentation tank prototype
The split type microreactor (miniature bioreactor, MBR) of micromation and Microfluid based Lab on a chip
(microfluid-based lab-on-a-chip).Although miniature organism reactor form is different, but all there is following functions
And feature: 1. multiparameter on-line checking function, can simultaneously pH value determination, DO (dissolved oxygen, dissolved oxygen), Pco2,
The important parameters such as OD (optical density, optical density);2. high throughput analysis function, integrated on a microreactor
Micro-fermentation tank number up to 6,12,24,48,96;3. volume is little, and its volume is generally less than 100mL, and have is the least extremely
5mL, micro-fluidic chip is then nL level scale.Additionally, also cost is low, reduce expensive raw materials consumption, reduce labor intensity etc.
Advantage.
In the embodiment one of miniature organism reactor of the present invention, miniature organism reactor includes tank body, shaft, tank
Lid, mechanical sealing linkage ring, mechanical sealing static ring and stirring paddle;Described mechanical sealing static ring is fixed on below cover;Shaft
Through the centre bore of cover, it is arranged in the inner chamber of tank body;Described mechanical sealing linkage ring, stirring paddle are arranged on shaft;?
Being additionally provided with combination type froth breaking oar as the aforementioned on shaft, wherein the structure of combination type froth breaking oar is: it include a pair blade,
Back-up ring, wherein, a pair blade is horizontally set on the outer ring of back-up ring, and blade place passes straight through the geometric center of back-up ring.This
The miniature organism reactor of bright offer, effectively can carry by simplifying the structure of froth breaking oar on the basis of meeting defoaming function
The high coefficient of miniature organism reactor, simplifies tank inner structure, it is simple to operate, clean, reduce dead angle risk simultaneously.
In the embodiment two of miniature organism reactor of the present invention, it is preferable that on the basis of above-described embodiment one, combination
Formula froth breaking oar is arranged on the bottom of mechanical sealing linkage ring, and it is solid that mechanical sealing linkage ring is pressed at the top of the back-up ring on combination type froth breaking oar
Fixed, to realize the axial limiting to mechanical sealing linkage ring.Back-up ring on combination type froth breaking oar is fixed on shaft by trip bolt
On, it can be adjusted flexibly according to spring compression degree needed for the axial accumulation mismachining tolerance of stirring system and mechanical sealing linkage ring
Position on shaft.
Preferably, combination type froth breaking oar position on shaft is adjustable, with the regulation pressure to described mechanical sealing linkage ring
Tight degree.After combination type froth breaking oar position on shaft determines, can be solid by combination type froth breaking oar by holding screw
On shaft.Can also stir so that combination type froth breaking oar is fixed on by back-up ring being fixed on shaft to wearing pin
Mix on axle, fix the crush to mechanical sealing linkage ring.
In a concrete preferred embodiment of miniature organism reactor of the present invention, with reference to Fig. 2, Fig. 3, miniature organism is anti-
Device is answered to include tank body 4, shaft 3, cover 6, mechanical sealing linkage ring 7 and stirring paddle 5;Shaft 3 is through the center of cover 6
Hole, is arranged in the inner chamber of tank body 4;Mechanical sealing linkage ring 7, stirring paddle 5 are arranged on shaft 3;Shaft 3 also sets up
Having combination type froth breaking oar, wherein, the structure of combination type froth breaking oar is: include blade 2, back-up ring 1, and a pair blade 2 is horizontally set on
The outer ring of back-up ring 1, blade 2 place passes straight through the geometric center of back-up ring 1.The bottom of blade 2 is provided with zigzag structure, and oar
The bottom of leaf 2 flushes with the bottom of back-up ring 1.Meanwhile, described combination type froth breaking oar is arranged on the bottom of mechanical sealing linkage ring 7, group
It is fixing that mechanical sealing linkage ring 7 is pressed at the top of the back-up ring 1 on box-like froth breaking oar.Wherein, combination type froth breaking oar is on shaft 3
Position is adjustable, with the regulation compression degree to mechanical sealing linkage ring 7.
In the present embodiment, the structure of stirring paddle can also use following structure: it includes wheel hub, disk and blade, oar
Leaf is structure as a whole with disk, and disk is fixed on the bottom of wheel hub.Blade, disk are the thin-wall metal of bend-resistance.Disk and wheel
Hub use rise connect, spot welding or bolt fastening connected mode.The middle part of disk is provided with under centre bore, central hole size and wheel hub
The excircle dimension in portion is equipped with.The quantity of blade is two to six, and it divides equally the outside at disk;Blade place plane and circle
Dish place plane is vertical.
The combination type froth breaking oar of present invention offer and the miniature organism reactor of use combination type froth breaking oar, its structure is tight
Gathering, rationally distributed, froth breaking oar uses fabricated structure with rotating ring back-up ring, on the basis of ensureing defoaming function, is effectively increased
The coefficient of miniature organism reactor, simplifies tank inner structure simultaneously, it is simple to operates, clean, reduce dead angle risk, the fullest
The foot use requirement of microreactor.
It should be noted that, above-described embodiment all can independent assortment as required.The above is only the preferred of the present invention
Embodiment, it is noted that for those skilled in the art, in the premise without departing from the principle of the invention
Under, it is also possible to making some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (7)
1. a combination type froth breaking oar, it is characterised in that including: a pair blade and back-up ring, the pair of blade is horizontally set on
The outer ring of back-up ring, and the pair of blade place passes straight through the geometric center of back-up ring.
Combination type froth breaking oar the most according to claim 1, it is characterised in that: the bottom of described blade is provided with zigzag knot
Structure, or flat impeller structure, or the combinative structure of orthogonal round bar and thin bar.
3. a miniature organism reactor, it is characterised in that: include that tank body, shaft, cover, mechanical sealing linkage ring, machinery are close
Envelope stationary ring and stirring paddle;Described mechanical sealing static ring is fixed on below cover;Shaft, through the centre bore of cover, is arranged on
In the inner chamber of tank body;Described mechanical sealing linkage ring, mechanical sealing static ring, stirring paddle are arranged on shaft;
Shaft is additionally provided with the arbitrary described combination type froth breaking oar of claim 1-2.
Miniature organism reactor the most according to claim 3, it is characterised in that: described combination type froth breaking oar is arranged on described
The bottom of mechanical sealing linkage ring, wherein, the top of the back-up ring on described combination type froth breaking oar press described mechanical sealing linkage ring with
Realize axial limiting.
Miniature organism reactor the most according to claim 3, it is characterised in that: described combination type froth breaking oar is in described stirring
Position on axle is adjustable, with the regulation compression degree to described mechanical sealing linkage ring.
Miniature organism reactor the most according to claim 5, it is characterised in that: when described combination type froth breaking oar stirs described
Mix after the position on axle determines, by holding screw, described combination type froth breaking oar is fixed on described shaft.
Miniature organism reactor the most according to claim 5, it is characterised in that: when described combination type froth breaking oar stirs described
Mix after the position on axle determines, by described back-up ring being fixed on described shaft, so that described combination type is disappeared wearing pin
Bubble oar is fixed on described shaft, fixes the crush to described mechanical sealing linkage ring.
Priority Applications (1)
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CN201610643053.4A CN106281978B (en) | 2016-08-08 | 2016-08-08 | A kind of combined type defoaming paddle and the miniature organism reactor using combined type defoaming paddle |
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CN201610643053.4A CN106281978B (en) | 2016-08-08 | 2016-08-08 | A kind of combined type defoaming paddle and the miniature organism reactor using combined type defoaming paddle |
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CN106281978A true CN106281978A (en) | 2017-01-04 |
CN106281978B CN106281978B (en) | 2019-04-09 |
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CN204981867U (en) * | 2015-09-09 | 2016-01-20 | 雅赛利(台州)制药有限公司 | Defoaming agitator |
CN105368704A (en) * | 2015-11-27 | 2016-03-02 | 上海日泰医药设备工程有限公司 | Fermentation tank |
CN205953998U (en) * | 2016-08-08 | 2017-02-15 | 上海国强生化工程装备有限公司 | Modular defoaming paddle and use combination formula defoaming paddle's miniature bioreactor |
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CN202139234U (en) * | 2011-06-30 | 2012-02-08 | 虞浩明 | Defoaming stirrer |
CN102373152A (en) * | 2011-10-18 | 2012-03-14 | 江苏浩特隆搅拌设备有限公司 | Stirrer combination for large-sized fermenting tank |
WO2015022852A1 (en) * | 2013-08-13 | 2015-02-19 | 三菱重工環境・化学エンジニアリング株式会社 | Defoaming device |
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