CN205874387U - Stirring rake and use miniature bioreactor of this stirring rake - Google Patents
Stirring rake and use miniature bioreactor of this stirring rake Download PDFInfo
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- CN205874387U CN205874387U CN201620850544.1U CN201620850544U CN205874387U CN 205874387 U CN205874387 U CN 205874387U CN 201620850544 U CN201620850544 U CN 201620850544U CN 205874387 U CN205874387 U CN 205874387U
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- disk
- stirring paddle
- stirring rake
- blade
- wheel hub
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Abstract
The utility model discloses a stirring rake and use miniature bioreactor of this stirring rake, a stirring rake, it includes wheel hub, disc and paddle, paddle and disc structure as an organic whole, the lower part at wheel hub is fixed to the disc. The utility model provides a pair of the stirring rake can bring following at least a beneficial effect: (1 )The stirring rake adopts the connected mode that rises and connect, effectively avoids conventional argon arc to weld the metal that brings and melts the defective problem of stream, has effectively guaranteed the dynamic balance ability of stirring rake, guarantees stirring rake pivoted stationarity, (2 )The stirring rake size of processing is accurate, has avoided causing the size difference after the stirring paddle leaf shaping because of the nondeterminacy of manufacture process, has reduced the influence to cultivation process and experimental result, is favorable to being used for miniature bioreactor to carry out the parallel test.
Description
Technical field
This utility model relates to microreactor stirring technique field, a kind of stirring paddle and use the micro-of this stirring paddle
Type bioreactor.
Background technology
In recent years, designative species has played increasing effect in national economy, receive more multimedia and
The concern of common people.The technology source of fermentable pharmacy is the strain of microorganism, and a good microorganism fungus kind is necessarily
The quality and quantity of the medicine that fermentable produces is determined in degree.And the High Throughput Screening Assay of strain is its performance improvement
One of important step.
At present, High Throughput Screening Assay obtains because its screening efficiency is high paying attention to and development day by day.And high flux screening,
During the micro updating reactor of laboratory amplifies research step by step to a liter stage reactor, need the miniature life through milliliter level
Thing reactor completes.
At biological technical field, existing lab scale, pilot scale and production-scale reactor, major part uses mechanical agitation system
System, to realize momentum transmission, heat transmission, quality transmission and the course of reaction in reactor.Mechanical agitation system uses motor
Drive shaft, drive stirring paddle and shaft synchronous rotary.Therefore, stirring system especially stirring paddle, is that stirring-type is biological
The vitals of reactor.
The most traditional stirring paddle uses blade, disk to form with wheel hub, and material uses stainless steel material to subtract as far as possible
Few metallic material corrosion leachable impact on bioprocesses.Blade, disk use stainless steel welded each other with wheel hub
The mode of conventional argon protection welding processes.
For the stirring paddle of miniature organism reactor, for reducing the interference stirred amplifying result of study as far as possible, also need
Dimension scale with reference to normal stirring paddle is designed and processes.Owing to disk is little with the size of blade, material is thin,
As used conventional argon arc welding, welding difficulty is relatively big, and easily causing welding effect, undesirable (melt flow affects overall dimensions, structure chi
Very little it is difficult to control to), on the one hand affect the dynamic balance performance of stirring paddle, and then affect the service life of stirring system；On the other hand,
The parallel test method that high flux screening is used, can cause each reactor because of the manufacturing variation of blade in each reactor
The bubble that interior dependence stirring paddle realizes is pulverized and is mixed with oxygen supply, medium and the index generation difference such as shearing force suffered by thalline, thus
Affect incubation based on parallel test scheme and experimental result, reduce the effectiveness of experimental data.
Utility model content
The purpose of this utility model is to provide a kind of stirring paddle and uses the miniature organism reactor of this stirring paddle, wherein stirs
The stirring paddle size mixing oar is accurate, it is to avoid because the uncertainty of manufacture process causes the size difference after stirrer paddle molding,
Reduce the impact on incubation and experimental result, be advantageously used for miniature organism reactor and carry out parallel test.
The technical scheme that this utility model provides is as follows:
A kind of stirring paddle, including: wheel hub, disk and blade, blade is structure as a whole with disk, and disk is fixed on wheel hub
Bottom.
It is further preferred that described blade, disk be can the thin-sheet metal of bending, with beneficially disk, the processing of blade
Molding.
Concrete, blade, the processing of disk use line excision forming, use thin-sheet metal, beneficially line cutting molybdenum wire
Select, facilitate the processing and forming of blade, disk；After using the processing of line excision forming, need bending and molding, i.e. by blade by water
Flat setting is bent into and is vertically arranged, and to expand the region of stirred fluid, reaches good mixing effect.
It is further preferred that described disk and wheel hub use rise connect, spot welding, thread connecting mode, these connected modes are easy
In control, the deficiency that welding manner brings, the problem i.e. avoiding metal melt flow defect so can be prevented effectively from.
Concrete, that disk and wheel hub are used easily controllable fixing and connected mode, connect as risen, spot welding, screw thread etc.
Mode, compared with welding manner, has certain advantage, and it can avoid the problem of metal melt flow defect, thus ensure that circle
Dish physical dimension, it is to avoid size distortion during machine-shaping, thus affect the design of the testing program in later stage.
It is further preferred that the middle part of described disk is provided with centre bore, outside central hole size is with wheel hub lower boss
Circle dimensional fits is arranged, and is fixed well ensureing that disk is accurately positioned with wheel hub and is connected.
Concrete, the centre bore of disk is equipped with the cylindrical of wheel hub lower boss, advantageously ensures that both positioning accurates
Degree and connect effect, i.e. in disk and wheel hub connection procedure, blade is accurately positioned by disk and wheel hub, it is ensured that stir
Mix the precision of paddle blade structure and size, to realize the concordance between each blade, thus be advantageously used for miniature organism reaction
Device carries out parallel test.
It is further preferred that the quantity of described blade be two leaves to or six leaves, its symmetry divides equally the outside at disk；Blade
Place plane and disk place plane vertically form flat leaf oar, it is possible to shape has a certain degree becomes oblique leaf oar.
Concrete, the outside of disk is provided with two to six, and it is uniformly arranged on the outside of disk；Blade and disk warp
Crossing after line cutting is integrally machined molding, blade and disk, on a horizontal plane, after line excision forming, need blade by level
Vertical or the certain angle position of position bending, is so conducive to blade to be fully contacted with the liquid of tank interior, it is ensured that stirring
The mixing effect of oar.
This utility model additionally provides a kind of miniature organism reactor, and it includes tank body, shaft, cover, froth breaking oar；Disappear
Bubble oar, through the centre bore of cover, is arranged in the inner chamber of tank body, and is arranged on shaft；Shaft is additionally provided with as
Aforesaid stirring paddle.
It is further preferred that described stirring paddle is arranged at described shaft one end close to tank base.
A kind of stirring paddle provided by this utility model and the miniature organism reactor using this stirring paddle, it is possible to bring
At least one beneficial effect below:
(1) stirring paddle uses easily controllable connection fixed form, connect as risen, spot welding, the mode such as screw thread, be prevented effectively from weldering
The problem of the metal melt flow defect that tape splicing comes, effectively reduces the unbalance dynamic quality of stirring paddle, it is ensured that stirring paddle is at miniature organism
The uniformity that reactor rotates；
(2) stirring paddle is rational in infrastructure, it is to avoid the stirring paddle difference impact on microbial cultivation process Yu result, favorably
In carrying out high flux bacterial screening by parallel experimental design scheme, improve the effectiveness of experimental data.
Accompanying drawing explanation
Below by the way of the most understandable, accompanying drawings preferred implementation, to a kind of stirring paddle and use should
Above-mentioned characteristic, technical characteristic, advantage and the implementation thereof of the miniature organism reactor of stirring paddle are further described.
Fig. 1 is the stirring paddle structural representation of this utility model；
Fig. 2 is disk and the paddle blade structure schematic diagram (non-bending) of this utility model；
Fig. 3 is the miniature organism structure of reactor schematic diagram using stirring paddle using this utility model to provide.
Drawing reference numeral illustrates:
1. wheel hub；2. disk；3. blade；4. tank body；5. shaft；6. cover；7. froth breaking oar；8. stirring paddle.
Detailed description of the invention
In order to be illustrated more clearly that this utility model embodiment or technical scheme of the prior art, accompanying drawing will be compareed below
Detailed description of the invention of the present utility model is described.It should be evident that the accompanying drawing in describing below is only of the present utility model one
A little embodiments, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to according to these
Accompanying drawing obtains other accompanying drawing, and obtains other embodiment.
For making simplified form, only schematically show the part relevant to this utility model in each figure, they are not
Represent its practical structures as product.It addition, so that simplified form readily appreciates, some figure has identical structure or merit
The parts of energy, only symbolically depict one of them, or have only marked one of them.In this article, " one " not only
Represent " only this ", it is also possible to represent the situation of " more than one ".
Fig. 1 is the structural representation of the embodiment one of this utility model stirring paddle, comprising: wheel hub 1, disk 2 and blade
3, wherein, blade 3 is structure as a whole with disk 2, and disk 2 is fixed on the bottom of wheel hub 1.
In another preferred embodiment of this utility model stirring paddle, blade, disk are can the thin-sheet metal of bending, it is easy to
Disk, the machine-shaping of blade.
In the further embodiment of this utility model stirring paddle, disk and wheel hub use easily controllable fixing and connect
Connect mode, connect as risen, spot welding, the mode such as screw thread, so can be prevented effectively from the deficiency that welding manner brings, i.e. avoid metal
The problem of melt flow defect.
In the another preferred embodiment of this utility model stirring paddle, the middle part of disk is provided with centre bore, centre bore chi
Very little and wheel hub bottom excircle dimension is equipped with, and is fixed well ensureing that disk is accurately positioned with wheel hub and is connected.
Fig. 2 is disk and the embodiment of paddle blade structure schematic diagram (non-bending) of this utility model, and blade 3 with disk 2 is
Integrative-structure, it uses line cutting mode to be formed；The quantity of blade 3 is four, and it divides equally the outside at disk 2, stirs to reduce
Mix the unbalance dynamic quality of oar, it is ensured that the rotational stability of stirring paddle；Blade 3 place plane is vertical with disk 2 place plane, this
Sample, beneficially blade are fully contacted with the liquid of tank interior, it is ensured that the mixing effect of stirring paddle.
The manufacturing process of the stirring paddle that this utility model provides is as follows:
First, choose the processing plate of disk 2 and blade 3, use the mode of line cutting, cut four according to design size
Centre bore in the middle part of blade 3 and disk 2；
Then, blade 3 is become vertical direction by horizontal direction knob, wherein, the direction of rotation phase of the blade 3 that position is relative
Instead；
Finally, use the method connect that rises, the external cylindrical surface of the centre bore in the middle part of disk 2 with wheel hub 1 bottom is connected fixing.
The invention also discloses a kind of miniature organism reactor.The miniature organism reactor occurred in the world at present exists
Three classes are generallyd include: orifice-plate type microreactor (microtiter plate, MTP), based on existing shaking flask or fermentation tank in structure
The split type microreactor (miniature bioreactor, MBR) of prototype micromation and Microfluid based Lab on a chip
(microfluid-based lab-on-a-chip).Although miniature organism reactor form is different, but all there is following functions
And feature: 1. multiparameter on-line checking function, can simultaneously pH value determination, DO (dissolved oxygen, dissolved oxygen), Pco2,
The important parameters such as OD (optical density, optical density)；2. high throughput analysis function, integrated on a microreactor
Micro-fermentation tank number up to 6,12,24,48,96；3. volume is little, and its volume is generally less than 100mL, and have is the least extremely
5mL, micro-fluidic chip is then nL level scale.Additionally, also cost is low, reduce expensive raw materials consumption, reduce labor intensity etc.
Advantage.
In the embodiment one of this utility model miniature organism reactor, miniature organism reactor include tank body, shaft,
Cover, froth breaking oar；Froth breaking oar, through the centre bore of cover, is arranged in the inner chamber of tank body, and is arranged on shaft；In stirring
Being additionally provided with stirring paddle on axle, wherein the structure of stirring paddle is: it includes wheel hub, disk and blade, and blade is integrated with disk
Structure, disk is fixed on the bottom of wheel hub.
In the embodiment two of this utility model miniature organism reactor, it is preferable that on the basis of above-described embodiment one,
Stirring paddle is arranged at described shaft one end close to tank base, it is simple to be stirred action, and stirring system is effectively ensured
Stable, the mixing effect of system operating uniform.
In a concrete preferred embodiment of this utility model miniature organism reactor, with reference to Fig. 1, Fig. 3, miniature life
Thing reactor includes tank body 4, shaft 5, cover 6, froth breaking oar 7；Froth breaking oar 7, through the centre bore of cover 6, is arranged on tank body 4
Inner chamber in, and be arranged on shaft 5；Being additionally provided with stirring paddle 8 on shaft 5, stirring paddle is fixed on by trip bolt
On shaft, stirring paddle 8 is arranged at shaft 5 close to the one end bottom tank body 4.Wherein, the structure of stirring paddle 8 is: its bag
Including wheel hub 1, disk 2 and blade 3, blade 3 is structure as a whole with disk 2, and disk 2 is fixed on the bottom of wheel hub 1.Blade 3, disk
2 is can the thin-sheet metal of bending.Disk 2 uses, with wheel hub 1, the connected mode connect that rises.The middle part of disk 2 is provided with centre bore, in
Central hole size is equipped with the excircle dimension of wheel hub 1 bottom.The quantity of blade 3 is four, and it divides equally the outside at disk 2；
Blade 3 place plane is vertical with disk 2 place plane.
In the present embodiment, the quantity of stirring paddle 8 is one, can arrange the quantity of stirring paddle 8 flexibly according to experiment needs.
In the present embodiment, the structure of froth breaking oar can also use following structure: it includes disk, back-up ring, pair of discs
Being horizontally set on the outer ring of back-up ring, disk place passes straight through the geometric center of back-up ring.The bottom of disk is provided with zigzag knot
Structure, and the bottom of disk flushes with the bottom of back-up ring.Meanwhile, described combination type froth breaking oar is arranged under mechanical sealing linkage ring
Portion, the top of the back-up ring on combination type froth breaking oar is pressed mechanical sealing linkage ring and is fixed.
A kind of stirring paddle provided by this utility model, it is possible to bring at least one beneficial effect following:
(1) stirring paddle uses the connected mode connect that rises, the problem being prevented effectively from the metal melt flow defect that welding brings, effectively
Reduce the unbalance dynamic quality of stirring paddle, it is ensured that the stability that stirring paddle rotates；
(2) stirring paddle is rational in infrastructure, it is to avoid between the stirring paddle of different reactor mutually difference to incubation with
The impact of experimental result, is conducive to improving the effectiveness of high flux bacterial screening experimental data.
It should be noted that, above-described embodiment all can independent assortment as required.Froth breaking oar in miniature organism reactor
Also the structure similar with this utility model can be taked to design.The above is only preferred implementation of the present utility model, it should
Point out, for those skilled in the art, on the premise of without departing from this utility model principle, it is also possible to do
Going out some improvements and modifications, these improvements and modifications also should be regarded as protection domain of the present utility model.
Claims (7)
1. a stirring paddle, it is characterised in that including: wheel hub, disk and blade, blade is structure as a whole with disk, and disk is solid
It is scheduled on the bottom of wheel hub.
Stirring paddle the most according to claim 1, it is characterised in that: described blade, disk are can the thin-sheet metal of bending.
Stirring paddle the most according to claim 1, it is characterised in that: described disk and wheel hub use to rise and connect or spot welding or screw thread
Mode connects.
Stirring paddle the most according to claim 1, it is characterised in that: the middle part of described disk is provided with centre bore, centre bore
Size is equipped with the excircle dimension of wheel hub bottom.
5. according to the arbitrary described stirring paddle of claim 1-4, it is characterised in that: the quantity of described blade is 2-6 part, and it is divided equally
Outside at disk；Blade place plane is vertical with disk place plane.
6. a miniature organism reactor, it is characterised in that: include tank body, shaft, cover, froth breaking oar；
Froth breaking oar, through the centre bore of cover, is arranged in the inner chamber of tank body, and is arranged on shaft；
Shaft is additionally provided with the arbitrary described stirring paddle of claim 1-5.
Miniature organism reactor the most according to claim 6, it is characterised in that: described stirring paddle is arranged at described shaft
One end close to tank base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620850544.1U CN205874387U (en) | 2016-08-08 | 2016-08-08 | Stirring rake and use miniature bioreactor of this stirring rake |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620850544.1U CN205874387U (en) | 2016-08-08 | 2016-08-08 | Stirring rake and use miniature bioreactor of this stirring rake |
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| Publication Number | Publication Date |
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| CN205874387U true CN205874387U (en) | 2017-01-11 |
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| CN201620850544.1U Active CN205874387U (en) | 2016-08-08 | 2016-08-08 | Stirring rake and use miniature bioreactor of this stirring rake |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085821A (en) * | 2016-08-08 | 2016-11-09 | 上海国强生化工程装备有限公司 | A kind of stirring paddle and use the miniature organism reactor of this stirring paddle |
-
2016
- 2016-08-08 CN CN201620850544.1U patent/CN205874387U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085821A (en) * | 2016-08-08 | 2016-11-09 | 上海国强生化工程装备有限公司 | A kind of stirring paddle and use the miniature organism reactor of this stirring paddle |
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