CN106281336A - Heavy metal passivation compositions and application thereof - Google Patents
Heavy metal passivation compositions and application thereof Download PDFInfo
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- CN106281336A CN106281336A CN201510246592.XA CN201510246592A CN106281336A CN 106281336 A CN106281336 A CN 106281336A CN 201510246592 A CN201510246592 A CN 201510246592A CN 106281336 A CN106281336 A CN 106281336A
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Abstract
The invention discloses heavy metal species passivation compositions and an application thereof.This heavy metal passivation compositions, its effective ingredient is made up of zeolite, red mud and Si-K fertilizer.In described heavy metal passivation compositions, the mass parts of zeolite is 20-30 part, specially 25 parts；The mass parts of red mud is 20-30 part, specially 25 parts；The mass parts of described Si-K fertilizer is 5-15 part, specially 10 parts.The invention provides the heavy metal passivation compositions that a kind of effective ingredient is zeolite, red mud and Si-K fertilizer.Test shows, slag can significantly reduce the Cd content in plant.It is applicable to various crops and vegetables orchard crop, is particularly suited for reducing the content of heavy metal Cd in Oryza sativa L. and Plantula Brassicae chinensis, there is important using value.
Description
Technical field
The present invention relates to heavy metal species passivation compositions and an application thereof.
Background technology
In recent years, China's heavy metal pollution is further serious, present industry to agricultural transfer, city to transfer of rural,
Earth's surface is shifted to underground, upstream toward downstream transfer, and the situation of transfer from water and soil pollution to food chain, by progressively accumulate
Pollution is going into sudden, linksystem, zonal outburst stage.Heavy metal pollution of soil is to ecological environment, food
Product safety and health constitute a serious threat.
On April 18th, 2014, Environmental Protection Department and Ministry of Land and Resources issued national Soil Pollution Investigation publication,
Points for investigation position covers all ploughs, part forest land, meadow, unused land and construction land.Result shows, whole nation soil
Earth environmental aspect totally allows of no optimist, and wherein arable land heavy metal pollution is especially serious, soil point position exceeding standard rate reaches 19.4%,
Far above forest land (10.0%) and the exceeding standard rate of meadow (10.4%).Exceed standard in terms of situation from pollutant, cadmium, hydrargyrum, arsenic,
Copper, lead, chromium, zinc, 8 kinds of inorganic pollution object point position exceeding standard rate of nickel are respectively 7.0%, 1.6%, 2.7%, 2.1%,
1.5%, 1.1%, 0.9%, 4.8%, the most serious with cadmium pollution.
Cadmium pollution not only has a strong impact on plant growth, and by the health even life of the food chain harm mankind.Poisonous
Heavy metal pollution course in agricultural land soil system has the feature of disguise, chronicity and irreversibility, it has also become
" the stealthy killer " of health.Research shows, cadmium is not the element of a kind of needed by human.Population passes through food
The quantity taking in Cd has exceeded the 90% of total exposed amount, and wherein rice, Semen Tritici aestivi, vegetable and Mollusca food are to day
The contribution taking in total cadmium reaches 40%～85%, and cadmium is considered as the heavy metal maximum to health toxicity, international cancer research
Cd was set to human carcinogen as far back as 1993 by office (IARC) the most, can cause mankind's renal carcinoma, bladder cancer, and
Breast carcinoma and carcinoma of prostate.
Based on this situation, China's heavy metal pollution of soil problem is increasingly paid much attention to by people, and is become one
Individual by the global problem of extensive concern.The decontamination technology of heavy metal-polluted soil receives much concern.Controlling of heavy metal pollution of soil
Manage and mainly have method, microorganism and the phytoremediation technology low costs such as biology, physics and chemistry, environmental friendliness, but by
Little in its target organism Biomass, repair time is long, is not suitable for agricultural land soil；Physical method easily operates, but cost is relatively
Height, is only suitable in little scope；Chemical in situ deactivation method low cost, easily operate, though can not thoroughly remove in soil
Heavy metal, but be applicable to large-scale farmland, selecting suitable passivator is then the key of chemical method.
Passivation heavy metal-polluted soil typically refers to contain the bigger heavy metal of the toxicity such as lead, cadmium, chromium, hydrargyrum in soil from easily
The Crop being planted on soil changes over and is difficult to by this Crop, to reduce heavy metal in soil to crop
Toxicity, thus ensure person poultry safety.
Due to China's intensive vegetable filed area expanding day, the technical need solving soil in protected field heavy metal pollution is very urgent,
Development facility field of vegetables heavy metal pollution Control Technology and environmentally friendly product, for realize the intensive vegetable filed high yield of China,
Stable yields and the sustainable use of field of vegetables, ensure crop production safety, have important practice significance and wide application
Prospect, is an important technical demand of outskirts of a town resources conservation and environmental harmony type agricultural development, is to advance socialism
One important support technology of new countryside construction.
Summary of the invention
It is an object of the invention to provide heavy metal species passivation compositions and an application thereof.
The heavy metal passivation compositions that the present invention provides, its effective ingredient is made up of zeolite, red mud and Si-K fertilizer.
Above-mentioned heavy metal passivation compositions also can be only made up of described zeolite, red mud and Si-K fertilizer.
In above-mentioned heavy metal passivation compositions, the mesh number of zeolite is 100-200 mesh, specially 100 mesh；
The mesh number of described Si-K fertilizer is 80-200 mesh, specially 100 mesh.
The zeolite of described zeolite concretely following main chemical compositions: silicon dioxide 65～69%, aluminium sesquioxide
12～13%, calcium oxide 1.8～3.3%, potassium chloride 1.1～3.1%, sodium oxide 0.3～2.6%, iron sesquioxide 0.6～1.7%,
Magnesium oxide 0.1～1.0%, titanium dioxide 0.07～0.13%.This zeolite is purchased from Chengde great zeolite factory.
Described red mud is the contaminative waste residue discharged during aluminium industry extraction aluminium oxide；
Concrete, in described red mud containing silicon dioxide, calcium oxide, aluminium sesquioxide, iron sesquioxide, manganese oxide,
Sodium oxide, potassium oxide and titanium oxide；
The red mud of the following composition of described red mud: the weight/mass percentage composition of silicon dioxide is 20～23%, oxidation
The weight/mass percentage composition of calcium is 46～49%, and the weight/mass percentage composition of aluminium sesquioxide is 5～7%, the matter of iron sesquioxide
Amount percentage composition is 7～10%, and the weight/mass percentage composition of manganese oxide is 1.2～1.6%, and the weight/mass percentage composition of sodium oxide is
2.0～2.5%, the weight/mass percentage composition of potassium oxide is 0.2～0.4%, and the weight/mass percentage composition of titanium oxide is 2.5～3.0%；
The Si-K fertilizer of Si-K fertilizer concretely following main chemical compositions: potassium oxide 4.5%, silicon dioxide 28.9%, oxygen
Change calcium 32.0%, effective zinc 0.0002%, effective ferrum 0.00064%, effective manganese 0.00125%.
In described heavy metal passivation compositions, the mass parts of zeolite is 20-30 part, specially 25 parts；
The mass parts of red mud is 20-30 part, specially 25 parts；
The mass parts of described Si-K fertilizer is 5-15 part, specially 10 parts.
The huge sum of money that described heavy metal passivation compositions is concretely made up of zeolite, red mud and the Si-K fertilizer of following mass ratio
Belong to and be passivated compositions: 25:25:10,20:30:10,30:20:10,25:30:5,30:25:5 or 20:
25:15.
What the present invention provided prepares the method that described heavy metal is passivated compositions, comprises the steps: aforementioned each component
Mix according to proportioning, obtain described heavy metal passivation compositions.
Above-mentioned heavy metal passivation compositions, as the application in heavy metal deactivator, falls within protection scope of the present invention.
The method reduced in plant in content of beary metal that the present invention provides, comprises the steps: the cultivation described plant
Training substrate adds the heavy metal passivation compositions that the aforementioned present invention provides.
In said method, described heavy metal passivation compositions is 12.5g-20g with the mass ratio of described cultivation of plants substrate:
1kg；The quality of described cultivation of plants substrate in terms of the quality of dry ground, specially 15g:1kg.
Described plant is crops or vegetables orchard crop；
Described crops are specially Oryza sativa L.；
Described vegetables orchard crop concretely Plantula Brassicae chinensis.
Described heavy metal concretely cadmium.
Described cultivation matrix concretely soil matrix.
Described being incorporated as disposably adds.
Content of beary metal during content of beary metal specially reduces the plant of described plant in described reduction plant, specifically may be used
For reducing the content of beary metal in rice plant or reducing the content of beary metal in Plantula Brassicae chinensis plant, can be more specifically to reduce
Content of beary metal in the rice plant of tillering stage and/or jointing stage.
The invention provides the heavy metal passivation compositions that a kind of effective ingredient is zeolite, red mud and Si-K fertilizer.Test table
Bright, slag can significantly reduce the Cd content in plant.It is applicable to various crops and vegetables orchard crop, especially
Be applicable to reducing the content of heavy metal Cd Oryza sativa L. and Plantula Brassicae chinensis, there is important using value.
Accompanying drawing explanation
Fig. 1 difference heavy metal resistance control material processes the impact on Plantula Brassicae chinensis Cd；
Fig. 2 difference heavy metal resistance control material processes the impact on Oryza sativa L. Cd in tillering stage；
Fig. 3 difference heavy metal resistance control material processes the impact on jointing stage Oryza sativa L. Cd；
In above-mentioned Fig. 1-Fig. 3, different letters all represent significant difference (P < 0.05).
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further elaborated, but the present invention is not limited to following example.Institute
Method of stating is conventional method if no special instructions.Described raw material the most all can be from open commercial sources
?.
Embodiment 1, the different heavy metal passivating material impact on Plantula Brassicae chinensis plant Cd
This test uses method for potted, test to enter outside Inst. of Plant Nutrition &. Resource, Beijing City Academy of Agricultural &. Fo greenhouse
OK.
Being moisture soil for examination soil, basis physicochemical property is moisture content 18.26%, pH7.44, rapid available phosphorus 8.64mg/kg,
Available potassium 95.01mg/kg, organic 0.728%, ammonium nitrogen 6.08mg/kg, nitrate nitrogen 46.29mg/kg, full nitrogen 0.023%.
5 process are divided in test, and the most different Cd hinders controls material, combination formula and comparison: zeolite, red mud,
Si-K fertilizer, combination formula (zeolite+red mud+Si-K fertilizer) and CK；It is repeated 4 times.
Zeolite is the zeolite powder of mistake 100 mesh sieve that Chengde great zeolite factory produces.Main chemical compositions is: silicon dioxide
65～69%, aluminium sesquioxide 12～13%, calcium oxide 1.8～3.3%, potassium chloride 1.1～3.1%, sodium oxide 0.3～2.6%,
Iron sesquioxide 0.6～1.7%, magnesium oxide 0.1～1.0%, titanium dioxide 0.07～0.13%.Cross 100 mesh sieves.
Si-K fertilizer crosses 100 mesh sieves.Main chemical compositions is potassium oxide 4.5%, silicon dioxide 28.9%, calcium oxide 32.0%,
Effectively zinc 0.0002%, effective ferrum 0.00064%, effective manganese 0.00125%.
Red mud is the contaminative waste residue discharged during aluminium industry extraction aluminium oxide, crosses 100 mesh sieves.Its main chemical compositions
For silicon dioxide 20～23%, calcium oxide 46～49%, aluminium sesquioxide 5～7%, iron sesquioxide 7～10%, oxidation
Manganese 1.2～1.6%, sodium oxide 2.0～2.5%, potassium oxide 0.2～0.4%, titanium oxide 2.5～3.0%.
Examination is Plantula Brassicae chinensis (kind: blue or green No. 1 of China) for crop.Potted-plant container is external diameter 25cm, the plastics of high 30cm
Basin.Dress soil on April 25th, 2014 (every basin 4kg (dry weight)), add cadmium nitrate and cultivate (interpolation concentration is 100ppm).
Each process dose is consistent, is chicken manure 200g, carbamide 0.4g, diamidogen 0.8g, potassium chloride 0.8g.
Different disposal resistance control material addition is as follows: zeolite 25g, red mud 25g, Si-K fertilizer 10g, combination formula
60g (zeolite 25g, red mud 25g, Si-K fertilizer 10g；Three kinds of materials are sufficiently mixed), CK without.
Meanwhile, the Cd passivation effect research adjusting formula proportion has also been carried out in this test, and concrete setting is shown in Table 1.
The setting of table 1, different ratio formula and the Cd passivation effect on Plantula Brassicae chinensis
Sowing on May 12nd, 2014, June 1 transplanted, every basin 2-3 strain.
Each process field management and period of duration topdress holding unanimously.Gathering in the crops on June 30th, 2014, plant is dried,
Cd is measured after grinding.The assay method of Cd is atomic absorption spectrophotometer (flame method).
Data arrange in excel, and the Cd content balance of different disposal uses one factor analysis of variance, multiple comparisons side
Method is LSD (P < 0.05).
Result of the test:
As it is shown in figure 1, add resistance control material each process plant Cd content the most in various degree less than CK process,
But only formula processes and has reached significance level (P < 0.05), illustrates to be applied in combination zeolite, red mud and Si-K fertilizer more have
It is beneficial to reduce Cd content in Plantula Brassicae chinensis.
Table 1 is the formula of the adjustment simultaneously the carried out passivation effect to Plantula Brassicae chinensis Cd, finds that the formula of different ratio is equal
Significantly reduce the Cd content in plant, but effect best remain the heavy metal that the present invention shown in Fig. 1 provides
Passivation composite formula (Cd content is 9.11mg/kg), namely by the zeolite of mass ratio 25:25:10, red mud and
The heavy metal passivation compositions of Si-K fertilizer composition.
Embodiment 2, the different heavy metal passivating material impact on rice plant Cd
Materials and methods
This test uses method for potted, test to enter outside Inst. of Plant Nutrition &. Resource, Beijing City Academy of Agricultural &. Fo greenhouse
OK.Being Guiyang, the Hunan rice soil by cadmium pollution for examination soil, basis physicochemical property is full nitrogen 3.63g/kg, organic
79.4g/kg, alkali-hydrolyzable nitrogen 226mg/kg, available phosphorus 38.7mg/kg, available potassium 126mg/kg, pH6.13.Test sets
Putting and dose is identical with test one with resistance control material addition, difference is that cadmium preparation (bag is the most artificially added in this test
Include the test adjusting formula, arrange and be shown in Table 2).
It is Oryza sativa L. (kind: Japan is fine) for studying thing, sows on April 9th, 2014.May 6 in 2014
Day, dress was native, and every basin 5.7kg weight in wet base, dry weight 4kg, Potted-plant container is external diameter 25cm, the plastic tub of high 30cm.
Rice transplanting on May 8, every basin 4 pier, the strain of every pier 5.Each process field management and period of duration topdress holding unanimously.
Respectively fetching water respectively at tillering stage on June 17th, 2014 and jointing stage July 17 rice 1 stem and leaf pier, plant takes back experiment
Room is dried, and pulverizes, and measures Cd content after grinding.Data statistical approach is with embodiment 1.
Result of the test:
Different disposal rice plant of tillering stage Cd content is as in figure 2 it is shown, be used alone zeolite and do not reduce plant Cd content
Play a role, and red mud, Si-K fertilizer and combined treatment all significantly reduce the content (P < 0.05) of Cd.Comparatively speaking,
The best results of combination formula.And in the jointing stage, zeolite shows Cd resistance control effect significantly, but show before
Preferably red mud lost efficacy；Si-K fertilizer and formula process and then significantly reduce Cd content in plant, and the effect of the latter is
Good.
Thus illustrate, be used alone red mud or Si-K fertilizer, the consistent effect of Oryza sativa L. Cd be there may be unstability.
And the effect of Si-K fertilizer and formula combination is relatively stable, and the latter's best results.
The setting of table 2, different ratio formula and the Cd passivation effect on Oryza sativa L.
Table 2 adjusts formula passivation effects to Oryza sativa L. Cd for other carried out simultaneously, it is seen that no matter tillering stage still
Jointing stage, the formula of different ratio all significantly reduces the Cd content in plant, but effect best remain Fig. 2,3
Shown in formula (tillering stage, Cd content was 0.079mg/kg, and the jointing stage is 0.055mg/kg), namely by mass ratio
The heavy metal passivation compositions of the zeolite of 25:25:10, red mud and Si-K fertilizer composition.
Integrated embodiment 1 is it can be seen that the Cd of crop is hindered control effect by the heavy metal passivation compositions that the present invention provides
Preferably, and have more extensive, the more stable suitability.
Claims (10)
1. a heavy metal species passivation compositions, its effective ingredient is made up of zeolite, red mud and Si-K fertilizer.
Heavy metal the most according to claim 1 passivation compositions, it is characterised in that: described heavy metal passivation group
Compound is made up of described zeolite, red mud and Si-K fertilizer.
Heavy metal the most according to claim 1 and 2 passivation compositions, it is characterised in that: the mesh of described zeolite
Number is 100-200 mesh, specially 100 mesh；
The mesh number of described Si-K fertilizer is 80-200 mesh, specially 100 mesh；
In described red mud containing silicon dioxide, calcium oxide, aluminium sesquioxide, iron sesquioxide, manganese oxide, sodium oxide,
Potassium oxide and titanium oxide.
Heavy metal the most according to claim 3 passivation compositions, it is characterised in that: described heavy metal passivation group
In compound, the mass parts of zeolite is 20-30 part, specially 25 parts；
The mass parts of red mud is 20-30 part, specially 25 parts；
The mass parts of described Si-K fertilizer is 5-15 part, specially 10 parts.
5. in claim 1-4, arbitrary described heavy metal is passivated compositions as the application in heavy metal deactivator.
6. prepare a method for arbitrary described heavy metal passivation compositions in claim 1-4, comprise the steps:
Described each component arbitrary in claim 1-4 is mixed according to proportioning, obtains described heavy metal passivation compositions.
7. reduce the method in content of beary metal in plant, comprise the steps: at described cultivation of plants base
Matter adds arbitrary described heavy metal passivation compositions in claim 1-4.
Method the most according to claim 7, it is characterised in that: described heavy metal passivation compositions is planted with described
The mass ratio of the cultivation matrix of thing is 12.5g-20g:1kg；The quality of described cultivation of plants substrate is with the quality of dry ground
Meter, specially 15g:1kg.
9. according to described method arbitrary in claim 6-8, it is characterised in that: described plant is crops or vegetables and fruits
Class crop；
Described crops are specially Oryza sativa L.；
Described vegetables orchard crop is specially Plantula Brassicae chinensis.
10. according to described method arbitrary in claim 6-9, it is characterised in that: described heavy metal is cadmium；Or,
Described cultivation matrix is soil matrix；Or,
Described being incorporated as disposably adds.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108580529A (en) * | 2018-04-11 | 2018-09-28 | 毛强平 | A kind of restorative procedure of heavy-metal contaminated soil |
| CN110079330A (en) * | 2018-01-26 | 2019-08-02 | 助听产业株式会社 | Manufacture composition, soil conditioner and the soil improvement method of soil conditioner |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103567219A (en) * | 2013-11-15 | 2014-02-12 | 中国农业科学院农业资源与农业区划研究所 | Multi-functional composite conditioner for controlling cadmium-polluted soil and remediation method for soil cadmium pollution |
-
2015
- 2015-05-14 CN CN201510246592.XA patent/CN106281336A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103567219A (en) * | 2013-11-15 | 2014-02-12 | 中国农业科学院农业资源与农业区划研究所 | Multi-functional composite conditioner for controlling cadmium-polluted soil and remediation method for soil cadmium pollution |
Non-Patent Citations (2)
| Title |
|---|
| 王立群等: "《不同钝化剂和培养时间对Cd污染土壤中可交换态Cd的影响》", 《农业环境科学》 * |
| 赵凤兰等: "《硅钾肥对青菜产量品质和土壤养分影响研究》", 《地域研究与开发》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110079330A (en) * | 2018-01-26 | 2019-08-02 | 助听产业株式会社 | Manufacture composition, soil conditioner and the soil improvement method of soil conditioner |
| CN108580529A (en) * | 2018-04-11 | 2018-09-28 | 毛强平 | A kind of restorative procedure of heavy-metal contaminated soil |
| CN108580529B (en) * | 2018-04-11 | 2020-07-03 | 江苏爱佳福如土壤修复有限公司 | Remediation method for heavy metal contaminated soil |
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