CN106279338A - The extracting method of Momordica charantial - Google Patents
The extracting method of Momordica charantial Download PDFInfo
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- CN106279338A CN106279338A CN201610628088.0A CN201610628088A CN106279338A CN 106279338 A CN106279338 A CN 106279338A CN 201610628088 A CN201610628088 A CN 201610628088A CN 106279338 A CN106279338 A CN 106279338A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J17/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta(a)hydrophenanthrene skeleton
- C07J17/005—Glycosides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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Abstract
The invention discloses the extracting method of a kind of Momordica charantial, comprise the following steps: 1) Fructus Momordicae charantiae is dried after pulverizing, with its quality 2～the petroleum ether soak degreasing 2 of 3 times～3 hours；2) during the Fructus Momordicae charantiae powder after defat adds 70%～80% ethanol solution, with the microwave radiation exaraction 30 of power 400～800W～after 90 seconds, 55～65 DEG C of water-baths are extracted 1～3 hour and repeat 2～4 times, merge gained filtrate, filtrate reduced in volume；3) adding activated carbon decolorizing in concentrated solution, filter, filtrate passes through resin column, first with 10～20 DEG C of washing posts, then with 60%～65% ethanol elution, after eluent concentrating under reduced pressure reclaims ethanol, is spray-dried and obtains finished product.The present invention can be during extracting Momordica charantial, roguing of effectively decolouring, thus prepares the product that purity is high, condition is good.
Description
Technical field
The present invention relates to the preparation method of plant extract, having is the extracting method of a kind of Momordica charantial.
Background technology
Fructus Momordicae charantiae has another name called balsam pear, half Fructus Cucumis flexuosi, is cucurbitaceous plant.Stem, branch, petiole and bennet have draped over one's shoulders pubescence, the raw tendril of axil.Leaf
The diameter 3 to 12 centimetres of son, has 5 to 7 road palmate drastic cracks, and sliver ovalize, there is sawtooth outer.At the end of spring and the beginning of summer bloom, female
Male homophyletic, yellow.Fruit oblong, surface has most irregular strumae.Seed is hidden among Fleshy fruits, ripe
Time have redness capsule wrap up in.Fructus Momordicae charantiae is a kind of vegetable, and original producton location is usually torrid areas.In South Asia, Southeast Asia, China and Galle
All have than extra large archipelago and plant widely.The traditional Chinese medical science is thought, Fructus Momordicae charantiae bitter in the mouth, cold in nature；GUIXIN, lung, spleen, stomach warp.There is heat clearing away of relieving summer heat, solve
Poison, effect of stomach invigorating.It is mainly used in heating, heatstroke, dysentery, conjunctival congestion and pain of eye, malignant boil etc..Pharmacological research shows, Momordica charantial is
One of effective ingredient in Fructus Momordicae charantiae, has higher medicinal and health value, the further investigation of Momordica charantial and develop further by
Have broad prospects.At present, the method extracting the sweet glycoside of Fructus Momordicae charantiae has a lot, but decolorizing effect is undesirable during extracting, and produces
Product purity is the highest, is difficult to be suitable for the requirement of industrial sweeting agent.
Summary of the invention
The technical problem to be solved in the present invention is to provide the extracting method of a kind of Momordica charantial, with prepared good decolorizing effect,
The Momordica charantial extract that purity is high.
For solve above-mentioned technical problem, the present invention by the following technical solutions:
The extracting method of Momordica charantial, comprises the following steps: be dried after 1) Fructus Momordicae charantiae is pulverized, dried Fructus Momordicae charantiae powder is used it
Quality 2～the petroleum ether soak degreasing 2 of 3 times～3 hours；2) during the Fructus Momordicae charantiae powder after defat adds 70%～80% ethanol solution,
Liquid ratio is 40～50:1 (mL/g), and with the microwave radiation exaraction 30 of power 400～800W～after 90 seconds, 55～65 DEG C of water-baths carry
Taking 1～3 hour and repeat 2～4 times, the most all crossing leaching filtrate, finally merge gained filtrate, filtrate reduced in volume reclaims second
Alcohol；3) concentrated solution adds the activated carbon of its weight 0.5%～2%, stirring decolouring 10～20 minutes, mistake at 65～70 DEG C
Filter, filtrate passes through resin column, and first with 10～20 DEG C of washing posts, then with 60%～65% ethanol elution, eluent concentrating under reduced pressure returns
After receiving ethanol, it is spray-dried and obtains finished product.
In technique scheme:
Step 1) in, described dry mode is: stop with the microwave heating 20 that power is 800～1200W～after 30 seconds
Heating 20～30 seconds, circulation is carried out to moisture content≤6%.The boiling range of described petroleum ether is preferably 30～60 DEG C.
Step 2) in, it is preferred that the volumetric concentration of described ethanol solution is 75%, and liquid ratio is 45:1 (mL/g)；Described
The power 600W of microwave, after the time of microwave radiation exaraction is 60 seconds；The temperature of described water-bath is 60 DEG C, and extraction time is 2 little
Time and be repeated 3 times.
Step 3) in, it is preferred that the addition of described activated carbon is the 1% of concentrated solution weight；Described resin column uses
HP100 macroporous resin, filtrate is 1.5～2BV/h by the flow velocity of resin column.
The invention have the benefit that
The present invention is by multiple steps such as petroleum ether degreasing, microwave-assisted alcohol extraction, activated carbon decolorizing, resin column essence carry, energy
Enough during extracting Momordica charantial, effectively go the removal of impurity, thus prepare the Momordica charantial extract that purity is high, condition is good.
Detailed description of the invention
Below in conjunction with specific embodiment for instruction and explanation of the present invention.
Embodiment 1
The extracting method of Momordica charantial, comprises the following steps:
1) it is dried after Fructus Momordicae charantiae is pulverized, the dried Fructus Momordicae charantiae powder petroleum ether (boiling range 30～60 DEG C) of its quality 3 times is soaked
Bubble defat 2.5 hours；The mode being dried is: with stopping heating 25 seconds after the microwave heating that power is 1000W 25 seconds, circulation is carried out
To moisture content≤6%；
2) during the Fructus Momordicae charantiae powder after defat adds 75% ethanol solution, liquid ratio is 45:1 (mL/g), micro-with power 600W
After ripple assisted extraction 60 seconds, 60 DEG C of water-baths are extracted 2 hours and are repeated 3 times, and the most all cross leaching filtrate, finally merge gained filter
Liquid, filtrate reduced in volume reclaims ethanol；
3) adding the activated carbon of its weight 1% in concentrated solution, at 68 DEG C, stirring decolouring 15 minutes, filter, and filtrate is passed through
HP100 macroporous resin column, filtrate flow velocity is 1.8BV/h, first with 15 DEG C of washing posts, then uses 65% ethanol elution, and eluent reduces pressure
After concentration and recovery ethanol, it is spray-dried and obtains finished product.
Embodiment 2
The extracting method of Momordica charantial, comprises the following steps:
1) it is dried after Fructus Momordicae charantiae is pulverized, the dried Fructus Momordicae charantiae powder petroleum ether (boiling range 30～60 DEG C) of its quality 2 times is soaked
Bubble defat 2.5 hours；The mode being dried is: with stopping heating 20 seconds after the microwave heating that power is 1200W 20 seconds, circulation is carried out
To moisture content≤6%；
2) during the Fructus Momordicae charantiae powder after defat adds 75% ethanol solution, liquid ratio is 50:1 (mL/g), micro-with power 400W
After ripple assisted extraction 90 seconds, 65 DEG C of water-baths are extracted 3 hours and are repeated 4 times, and the most all cross leaching filtrate, finally merge gained filter
Liquid, filtrate reduced in volume reclaims ethanol；
3) adding the activated carbon of its weight 2% in concentrated solution, at 65 DEG C, stirring decolouring 20 minutes, filter, and filtrate is passed through
HP100 macroporous resin column, filtrate flow velocity is 2BV/h, first with 10 DEG C of washing posts, then uses 60% ethanol elution, and eluent decompression is dense
Retract after receiving ethanol, be spray-dried and obtain finished product.
Embodiment 3
The extracting method of Momordica charantial, comprises the following steps:
1) it is dried after Fructus Momordicae charantiae is pulverized, the dried Fructus Momordicae charantiae powder petroleum ether (boiling range 30～60 DEG C) of its quality 3 times is soaked
Bubble defat 2 hours；The mode being dried is: with stopping heating 30 seconds after the microwave heating that power is 800W 30 seconds, circulation carry out to
Moisture content≤6%；
2) during the Fructus Momordicae charantiae powder after defat adds 80% ethanol solution, liquid ratio is 40:1 (mL/g), micro-with power 800W
After ripple assisted extraction 60 seconds, 55 DEG C of water-baths are extracted 1 hour and are repeated 4 times, and the most all cross leaching filtrate, finally merge gained filter
Liquid, filtrate reduced in volume reclaims ethanol；
3) adding the activated carbon of its weight 0.5% in concentrated solution, at 70 DEG C, stirring decolouring 10 minutes, filter, and filtrate is led to
Crossing HP100 macroporous resin column, filtrate flow velocity is 2BV/h, first with 15 DEG C of washing posts, then uses 65% ethanol elution, and eluent reduces pressure
After concentration and recovery ethanol, it is spray-dried and obtains finished product.
Embodiment 4
The extracting method of Momordica charantial, comprises the following steps:
1) it is dried after Fructus Momordicae charantiae is pulverized, the dried Fructus Momordicae charantiae powder petroleum ether (boiling range 30～60 DEG C) of its quality 2 times is soaked
Bubble defat 3 hours；The mode being dried is: with stopping heating 25 seconds after the microwave heating that power is 900W 25 seconds, circulation carry out to
Moisture content≤6%；
2) during the Fructus Momordicae charantiae powder after defat adds 70% ethanol solution, liquid ratio is 50:1 (mL/g), micro-with power 400W
After ripple assisted extraction 60 seconds, 60 DEG C of water-baths are extracted 3 hours and are repeated 2 times, and the most all cross leaching filtrate, finally merge gained filter
Liquid, filtrate reduced in volume reclaims ethanol；
3) adding the activated carbon of its weight 2% in concentrated solution, at 70 DEG C, stirring decolouring 10 minutes, filter, and filtrate is passed through
HP100 macroporous resin column, filtrate flow velocity is 1.5BV/h, first with 20 DEG C of washing posts, then uses 60% ethanol elution, and eluent reduces pressure
After concentration and recovery ethanol, it is spray-dried and obtains finished product.
Claims (6)
1. the extracting method of Momordica charantial, it is characterised in that comprise the following steps:
1) it is dried after Fructus Momordicae charantiae is pulverized, by dried Fructus Momordicae charantiae powder its quality 2～the petroleum ether soak degreasing 2 of 3 times～3 hours；
2), during the Fructus Momordicae charantiae powder after defat adds 70%～80% ethanol solution, liquid ratio is 40～50:1 (mL/g), with power 400
～the microwave radiation exaraction 30 of 800W～after 90 seconds, 55～65 DEG C of water-baths are extracted 1～3 hour and repeat 2～4 times, the most all mistakes
Leaching filtrate, finally merges gained filtrate, and filtrate reduced in volume reclaims ethanol；
3) concentrated solution adds the activated carbon of its weight 0.5%～2%, stirring decolouring 10～20 minutes, mistake at 65～70 DEG C
Filter, filtrate passes through resin column, and first with 10～20 DEG C of washing posts, then with 60%～65% ethanol elution, eluent concentrating under reduced pressure returns
After receiving ethanol, it is spray-dried and obtains finished product.
Extracting method the most according to claim 1, it is characterised in that step 1) in, described dry mode is: use power
Be 800～1200W microwave heating 20～after 30 seconds stop heating 20～30 seconds, circulation carry out to moisture content≤6%.
Extracting method the most according to claim 1, it is characterised in that: step 1) in, the boiling range of described petroleum ether be 30～
60℃。
Extracting method the most according to claim 1, it is characterised in that: step 2) in, the volumetric concentration of described ethanol solution
Being 75%, liquid ratio is 45:1 (mL/g)；The power 600W of described microwave, after the time of microwave radiation exaraction is 60 seconds；Described
The temperature of water-bath is 60 DEG C, and extraction time is 2 hours and is repeated 3 times.
Extracting method the most according to claim 1, it is characterised in that: step 3) in, the addition of described activated carbon is dense
The 1% of contracting liquid weight.
Extracting method the most according to claim 1, it is characterised in that: step 3) in, described resin column uses HP100 macropore
Resin, filtrate is 1.5～2BV/h by the flow velocity of resin column.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610628088.0A CN106279338A (en) | 2016-08-03 | 2016-08-03 | The extracting method of Momordica charantial |
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| CN201610628088.0A CN106279338A (en) | 2016-08-03 | 2016-08-03 | The extracting method of Momordica charantial |
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| CN201610628088.0A Pending CN106279338A (en) | 2016-08-03 | 2016-08-03 | The extracting method of Momordica charantial |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106038666A (en) * | 2016-07-29 | 2016-10-26 | 桂林实力科技有限公司 | Bitter melon leaf extract and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101880303A (en) * | 2010-04-27 | 2010-11-10 | 南京泽朗农业发展有限公司 | Method for extracting bitter gourd saponin from bitter gourd |
| CN102030795A (en) * | 2010-11-30 | 2011-04-27 | 哈尔滨商业大学 | Cucurbitane momordicoside and preparation method thereof |
-
2016
- 2016-08-03 CN CN201610628088.0A patent/CN106279338A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101880303A (en) * | 2010-04-27 | 2010-11-10 | 南京泽朗农业发展有限公司 | Method for extracting bitter gourd saponin from bitter gourd |
| CN102030795A (en) * | 2010-11-30 | 2011-04-27 | 哈尔滨商业大学 | Cucurbitane momordicoside and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 唐彦君等: "微波辅助提取苦瓜总皂苷工艺条件优化", 《黑龙江八一农垦大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106038666A (en) * | 2016-07-29 | 2016-10-26 | 桂林实力科技有限公司 | Bitter melon leaf extract and application thereof |
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Application publication date: 20170104 |
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