CN106267306A - A kind of preparation method of antibiotic property bacteria cellulose film - Google Patents
A kind of preparation method of antibiotic property bacteria cellulose film Download PDFInfo
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- CN106267306A CN106267306A CN201610646439.0A CN201610646439A CN106267306A CN 106267306 A CN106267306 A CN 106267306A CN 201610646439 A CN201610646439 A CN 201610646439A CN 106267306 A CN106267306 A CN 106267306A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/402—Anaestetics, analgesics, e.g. lidocaine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
Abstract
The invention discloses the preparation method of a kind of antibiotic property bacteria cellulose film, belong to Bacterial cellulose technical field of membrane.The present invention carries out enzymatic saccharification to wheat stalk, nicotiamide is added in saccharifying, as luring the factor, again by high pressure along quick-fried process, preparation fermentation concentrated solution, activated by acetobacter xylinum subsequently, again with Nutrious fermented things such as concentrated solution, nutrient, chitosans, ferment, with nicotiamide for induction, film loading chitosan, it is prepared into antibiotic property bacteria cellulose film, compensate for the defect that conventional bacteria cellulose membrane anti-microbial property is the best, more can effectively make bacteria cellulose film keep long-acting bacteriostatic, there is wider application prospect.
Description
Technical field
The invention discloses the preparation method of a kind of antibiotic property bacteria cellulose film, belong to Bacterial cellulose membrane technology neck
Territory.
Background technology
Bacterial cellulose, as a kind of excellent biomaterial, has a process based prediction model of its uniqueness: bacterial fibers
Element has three-dimensional network-like structure, high retentiveness, water absorption and breathability, high-tensile and elastic modelling quantity.Numerous studies table
Bright, Bacterial cellulose has good biocompatibility and biodegradability, energy alleviating pain, promotes wound healing, does not contains
Toxicant.Simple Bacterial cellulose as dressing for skin abroad it has been reported that and have been used for clinic, and the most domestic right
Bacterial cellulose applied research in terms of dressing for skin also gets more and more.Bacterial cellulose itself has good water absorption,
Transudate and the metabolite of wound, and Bacterial cellulose can be continuously and effectively absorbed on the basis of ensureing biological safety
Also there is good breathability, promote the healing of wound.
Bacterial cellulose is the fiber substance that the carbohydrate of little molecule is formed through fermentable.With traditional plant
Cellulose has compared many premium properties, such as high-purity, high polymerization degree, noble and unsullied crystalline substance degree, high-hydrophilic, higher biocompatibility
Deng, can directly degrade at nature.Therefore, with Bacterial cellulose as raw materials in producing films, not only widened cellulosic material
Source, and thus obtained film strength is high, biodegradable, it is possible to meet the demand of current environmental protection packing business.Carefully
Fungin is as a kind of novel Microbe synthesis material, at food industry, biomedicine, papermaking, acoustics equipment and oil
Exploitation aspect has been obtained for being widely applied.Technology of preparing about Bacterial cellulose is the most much reported at present, mainly
Its application in terms of dressing is studied by traditional quiescent culture.The antibacterial obtained by the method for tradition quiescent culture is fine
The structure that dimension element film has is single, and have then causes bacteria cellulose film uneven due to employing machine cuts, and by traditional
The Bacterial cellulose film thickness that cultural method obtains can not be controlled effectively.Conventional bacteria cellulose membrane exists antibacterial at present
The defect that performance is the best, causes using limitation urgently to be resolved hurrily.
Summary of the invention
The technical problem that present invention mainly solves: the best for the most traditional bacteria cellulose film anti-microbial property, causes
Depositing the defect that in use there is significant ASIC limitation, the present invention carries out enzymatic saccharification to wheat stalk, in saccharifying
Add nicotiamide, as luring the factor, then by high pressure along quick-fried process, prepare fermentation concentrated solution, pass through acetobacter xylinum subsequently
Activation, with the Nutrious fermented thing such as concentrated solution, nutrient, chitosan, ferment, with nicotiamide for induction, film load shell gathers
Sugar, is prepared into antibiotic property bacteria cellulose film, compensate for the defect that conventional bacteria cellulose membrane anti-microbial property is the best, more can have
The bacteria cellulose film that makes of effect keeps long-acting bacteriostatic, has wider application prospect.
In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is:
(1) taking wheat stalk and put in baking oven, design temperature is 40～45 DEG C, is dried 1～2h, then pulverizes, and crosses 100 mesh
Sieve, obtains wheat stalk granule, by solid-to-liquid ratio 1:3, is that 10% nicotinamide soln puts into enzyme by wheat stalk granule and mass fraction
Solving in tank, using mass fraction is 30% sulfuric acid solution regulation pH to 5.5～6.0, stirs 40～45min with 150r/min；
(2) after above-mentioned stirring terminates stand 15～20min, then in enzymatic vessel add wheat stalk granular mass 2.2～
The mixed enzyme of 2.5%, design temperature is 35～45 DEG C, stirs enzymolysis 4～6h with 140r/min, naturally cools to room temperature subsequently,
Enzymolysis mixture is put in autoclave, boosts to 3～4MPa, pressurize 3～5min, it is depressurized to mark 1～3min subsequently
Quasi-atmospheric pressure discharging, collect discharging thing, and put in centrifuge, is centrifuged 10～15min with 3000r/min, collects supernatant,
It is further concentrated to the 45～48% of original volume, collects concentrated solution, standby；Described mixed enzyme is that xylanase and catalase are by matter
Amount mixes than 3:1；
(3) by inoculum concentration 10～12%, acetobacter xylinum is seeded to beef extract-peptone agar culture medium, is placed in constant-temperature table
In, design temperature is 30～35 DEG C, with 150rpm shaken cultivation 14～19h, subsequently by culture medium take out, use sterilized water with
The speed drip washing media surface of 4mL/min, to its surface sterile silk, is collected leacheate, is obtained bacterium solution；
(4) count by weight, take 40～50 parts of standby concentrated solutions of step (2), 18～22 parts of peptones, 14～16 parts of yeast
Extractum, 8～12 parts of chitosans, 2～3 parts of citric acids, 1～2 part of magnesium sulfate and 0.8～1.4 part of potassium dihydrogen phosphate, stir,
And sterilizing, obtain Nutrious fermented thing, Nutrious fermented thing is added to fermentation tank, by inoculum concentration 12～16%, to fermentation tank
The above-mentioned bacterium solution of middle addition, design temperature is 28～35 DEG C, and ventilation is 3～5vvm, standing for fermentation 3～6 days；
(5) after above-mentioned fermentation ends, use tweezers by the antibiotic property bacteria cellulose film on fermentation cylinder for fermentation mixture surface
Take out, be soaked in 1.5mol/L sodium hydroxide solution, after standing 1～2h at 88～94 DEG C, filtered while hot, use distillation
Water washing antibiotic property bacteria cellulose film is to neutral, subsequently by its natural air drying, and sterilizing, antibiotic property antibacterial is fine
Dimension element film.
The physical property of the present invention is: the antibiotic property Bacterial cellulose film thickness of gained of the present invention be 0.200～
0.400mm, membrane aperture is 10～12nm, and colibacillary antibiotic rate is reached 98～99%.
The invention has the beneficial effects as follows:
(1) bacteria cellulose film that the present invention obtains is more loose compared to its structure of bacteria cellulose film that tradition cultivation obtains,
Having good retentiveness and breathability as human body skin dressing, evenly, mechanical property is more preferable for film；
(2) the antibacterial cellulose film that the present invention prepares relatively conventional bacteria cellulose membrane antibiotic rate improves 6～8 times, and anti-microbial property is relatively
Good.
Detailed description of the invention
First taking wheat stalk and put in baking oven, design temperature is 40～45 DEG C, is dried 1～2h, then pulverizes, mistake
100 mesh sieves, obtain wheat stalk granule, by solid-to-liquid ratio 1:3, are that 10% nicotinamide soln is put by wheat stalk granule and mass fraction
Enter in enzymatic vessel, use mass fraction be 30% sulfuric acid solution regulation pH to 5.5～6.0, with 150r/min stirring 40～
45min；After above-mentioned stirring terminates stand 15～20min, then in enzymatic vessel add wheat stalk granular mass 2.2～
The mixed enzyme of 2.5%, design temperature is 35～45 DEG C, stirs enzymolysis 4～6h with 140r/min, naturally cools to room temperature subsequently,
Enzymolysis mixture is put in autoclave, boosts to 3～4MPa, pressurize 3～5min, it is depressurized to mark 1～3min subsequently
Quasi-atmospheric pressure discharging, collect discharging thing, and put in centrifuge, is centrifuged 10～15min with 3000r/min, collects supernatant,
It is further concentrated to the 45～48% of original volume, collects concentrated solution, standby；Described mixed enzyme is that xylanase and catalase are by matter
Amount mixes than 3:1；By inoculum concentration 10～12%, acetobacter xylinum is seeded to beef extract-peptone agar culture medium, juxtaposition
In constant-temperature table, design temperature is 30～35 DEG C, with 150rpm shaken cultivation 14～19h, culture medium is taken out subsequently, uses
Sterilized water, with the speed drip washing media surface of 4mL/min to its surface sterile silk, is collected leacheate, is obtained bacterium solution；By weight
Number meter, take 40～50 parts of standby concentrated solutions, 18～22 parts of peptones, 14～16 parts of yeast extracts, 8～12 parts of chitosans, 2～
3 parts of citric acids, 1～2 part of magnesium sulfate and 0.8～1.4 part of potassium dihydrogen phosphate, stir, and sterilizing, obtains Nutrious fermented
Thing, adds Nutrious fermented thing to fermentation tank, by inoculum concentration 12～16%, adds above-mentioned bacterium solution in fermentation tank, sets temperature
Degree is 28～35 DEG C, and ventilation is 3～5vvm, standing for fermentation 3～6 days；After above-mentioned fermentation ends, use tweezers by fermentation tank
The antibiotic property bacteria cellulose film on middle fermenting mixture surface takes out, and is soaked in 1.5mol/L sodium hydroxide solution,
After 88～94 DEG C stand 1～2h, filtered while hot, use distilled water wash antibiotic property bacteria cellulose film the most neutral, subsequently by it
Natural air drying, and sterilizing, antibiotic property bacteria cellulose film.
Example 1
First taking wheat stalk and put in baking oven, design temperature is 40 DEG C, is dried 1h, then pulverizes, and crosses 100 mesh sieves, obtains little
Wheat Straw granule, by solid-to-liquid ratio 1:3, is that 10% nicotinamide soln is put in enzymatic vessel by wheat stalk granule and mass fraction,
Using mass fraction is 30% sulfuric acid solution regulation pH to 5.5, stirs 40min with 150r/min；After above-mentioned stirring terminates quiet
Putting 15min, then the mixed enzyme of addition wheat stalk granular mass 2.2% in enzymatic vessel, design temperature is 35 DEG C, with 140r/
Min stirs enzymolysis 4h, naturally cools to room temperature subsequently, puts in autoclave by enzymolysis mixture, boosts to 3MPa, pressurize
3min, is depressurized to normal atmosphere discharging at 1min subsequently, collects discharging thing, and puts in centrifuge, with 3000r/min from
Heart 10min, collects supernatant, is further concentrated to the 45% of original volume, collects concentrated solution, standby；Described mixed enzyme is xylanase
Mix with catalase 3:1 in mass ratio；By inoculum concentration 10%, acetobacter xylinum is seeded to beef extract-peptone agar
Culture medium, is placed in constant-temperature table, and design temperature is 30 DEG C, with 150rpm shaken cultivation 14h, culture medium is taken out subsequently,
Use sterilized water with the speed drip washing media surface of 4mL/min to its surface sterile silk, collect leacheate, obtain bacterium solution；By weight
Amount number meter, takes 40 parts of standby concentrated solutions, 18 parts of peptones, 14 parts of yeast extracts, 8 parts of chitosans, 2 parts of citric acids, 1 part of sulfur
Acid magnesium and 0.8 part of potassium dihydrogen phosphate, stir, and sterilizing, obtains Nutrious fermented thing, adds Nutrious fermented thing to fermentation
In tank, by inoculum concentration 12%, adding above-mentioned bacterium solution in fermentation tank, design temperature is 28 DEG C, and ventilation is 3vvm, standing for fermentation
3 days；After above-mentioned fermentation ends, tweezers are used to be taken by the antibiotic property bacteria cellulose film on fermentation cylinder for fermentation mixture surface
Go out, be soaked in 1.5mol/L sodium hydroxide solution, after standing 1h at 88 DEG C, filtered while hot, use distilled water wash to resist
Bacterium property bacteria cellulose film is to neutral, subsequently by its natural air drying, and sterilizing, antibiotic property bacteria cellulose film.
The physical property of the present invention is: the antibiotic property Bacterial cellulose film thickness of gained of the present invention is 0.200mm, membrane aperture
For 10nm, colibacillary antibiotic rate is reached 98%.
Example 2
First taking wheat stalk and put in baking oven, design temperature is 43 DEG C, is dried 1.5h, then pulverizes, and crosses 100 mesh sieves,
Wheat stalk granule, by solid-to-liquid ratio 1:3, is that 10% nicotinamide soln puts into enzymatic vessel by wheat stalk granule and mass fraction
In, using mass fraction is 30% sulfuric acid solution regulation pH to 5.7, stirs 43min with 150r/min；After above-mentioned stirring terminates
Standing 17min, then the mixed enzyme of addition wheat stalk granular mass 2.4% in enzymatic vessel, design temperature is 40 DEG C, with
140r/min stirs enzymolysis 5h, naturally cools to room temperature subsequently, puts in autoclave by enzymolysis mixture, boost to
3.5MPa, pressurize 4min, be depressurized to normal atmosphere discharging at 2min subsequently, collects discharging thing, and puts in centrifuge, with
3000r/min is centrifuged 13min, collects supernatant, is further concentrated to the 47% of original volume, collects concentrated solution, standby；Described mixed enzyme
Mix for xylanase and catalase 3:1 in mass ratio；By inoculum concentration 11%, acetobacter xylinum is seeded to Carnis Bovis seu Bubali cream
Peptone agar culture medium, is placed in constant-temperature table, and design temperature is 33 DEG C, with 150rpm shaken cultivation 17h, and subsequently will training
Support base to take out, use sterilized water with the speed drip washing media surface of 4mL/min to its surface sterile silk, collect leacheate,
Bacterium solution；Count by weight, take 45 parts of standby concentrated solutions, 20 parts of peptones, 15 parts of yeast extracts, 10 parts of chitosans, 2.5 parts
Citric acid, 1.5 parts of magnesium sulfate and 1.0 parts of potassium dihydrogen phosphates, stir, and sterilizing, obtains Nutrious fermented thing, fermentation is sought
Supporting thing to add to fermentation tank, by inoculum concentration 14%, add above-mentioned bacterium solution in fermentation tank, design temperature is 31 DEG C, ventilation
For 4vvm, standing for fermentation 5 days；After above-mentioned fermentation ends, use tweezers by the antibiotic property on fermentation cylinder for fermentation mixture surface
Bacteria cellulose film takes out, and is soaked in 1.5mol/L sodium hydroxide solution, after standing 1.5h at 91 DEG C, and filtered while hot,
Use distilled water wash antibiotic property bacteria cellulose film to neutral, subsequently by its natural air drying, and sterilizing, antibacterial
Property bacteria cellulose film.
The physical property of the present invention is: the antibiotic property Bacterial cellulose film thickness of gained of the present invention is 0.300mm, membrane aperture
For 11nm, colibacillary antibiotic rate is reached 98.5%.
Example 3
First taking wheat stalk and put in baking oven, design temperature is 45 DEG C, is dried 2h, then pulverizes, and crosses 100 mesh sieves, obtains little
Wheat Straw granule, by solid-to-liquid ratio 1:3, is that 10% nicotinamide soln is put in enzymatic vessel by wheat stalk granule and mass fraction,
Using mass fraction is 30% sulfuric acid solution regulation pH to 6.0, stirs 45min with 150r/min；After above-mentioned stirring terminates quiet
Putting 20min, then the mixed enzyme of addition wheat stalk granular mass 2.5% in enzymatic vessel, design temperature is 45 DEG C, with 140r/
Min stirs enzymolysis 6h, naturally cools to room temperature subsequently, puts in autoclave by enzymolysis mixture, boosts to 4MPa, pressurize
5min, is depressurized to normal atmosphere discharging at 3min subsequently, collects discharging thing, and puts in centrifuge, with 3000r/min from
Heart 15min, collects supernatant, is further concentrated to the 48% of original volume, collects concentrated solution, standby；Described mixed enzyme is xylanase
Mix with catalase 3:1 in mass ratio；By inoculum concentration 12%, acetobacter xylinum is seeded to beef extract-peptone agar
Culture medium, is placed in constant-temperature table, and design temperature is 35 DEG C, with 150rpm shaken cultivation 19h, culture medium is taken out subsequently,
Use sterilized water with the speed drip washing media surface of 4mL/min to its surface sterile silk, collect leacheate, obtain bacterium solution；By weight
Amount number meter, take 50 parts of standby concentrated solutions, 22 parts of peptones, 16 parts of yeast extracts, 12 parts of chitosans, 3 parts of citric acids, 2 parts
Magnesium sulfate and 1.4 parts of potassium dihydrogen phosphates, stir, and sterilizing, obtains Nutrious fermented thing, adds Nutrious fermented thing to sending out
In ferment tank, by inoculum concentration 16%, adding above-mentioned bacterium solution in fermentation tank, design temperature is 35 DEG C, and ventilation is 5vvm, stands and sends out
Ferment 6 days；After above-mentioned fermentation ends, tweezers are used to be taken by the antibiotic property bacteria cellulose film on fermentation cylinder for fermentation mixture surface
Go out, be soaked in 1.5mol/L sodium hydroxide solution, after standing 2h at 94 DEG C, filtered while hot, use distilled water wash to resist
Bacterium property bacteria cellulose film is to neutral, subsequently by its natural air drying, and sterilizing, antibiotic property bacteria cellulose film.
The physical property of the present invention is: the antibiotic property Bacterial cellulose film thickness of gained of the present invention is 0.400mm, membrane aperture
For 12nm, colibacillary antibiotic rate is reached 99%.
Claims (1)
1. the preparation method of an antibiotic property bacteria cellulose film, it is characterised in that concrete preparation process is:
(1) taking wheat stalk and put in baking oven, design temperature is 40～45 DEG C, is dried 1～2h, then pulverizes, and crosses 100 mesh
Sieve, obtains wheat stalk granule, by solid-to-liquid ratio 1:3, is that 10% nicotinamide soln puts into enzyme by wheat stalk granule and mass fraction
Solving in tank, using mass fraction is 30% sulfuric acid solution regulation pH to 5.5～6.0, stirs 40～45min with 150r/min；
(2) after above-mentioned stirring terminates stand 15～20min, then in enzymatic vessel add wheat stalk granular mass 2.2～
The mixed enzyme of 2.5%, design temperature is 35～45 DEG C, stirs enzymolysis 4～6h with 140r/min, naturally cools to room temperature subsequently,
Enzymolysis mixture is put in autoclave, boosts to 3～4MPa, pressurize 3～5min, it is depressurized to mark 1～3min subsequently
Quasi-atmospheric pressure discharging, collect discharging thing, and put in centrifuge, is centrifuged 10～15min with 3000r/min, collects supernatant,
It is further concentrated to the 45～48% of original volume, collects concentrated solution, standby；Described mixed enzyme is that xylanase and catalase are by matter
Amount mixes than 3:1；
(3) by inoculum concentration 10～12%, acetobacter xylinum is seeded to beef extract-peptone agar culture medium, is placed in constant-temperature table
In, design temperature is 30～35 DEG C, with 150rpm shaken cultivation 14～19h, subsequently by culture medium take out, use sterilized water with
The speed drip washing media surface of 4mL/min, to its surface sterile silk, is collected leacheate, is obtained bacterium solution；
(4) count by weight, take 40～50 parts of standby concentrated solutions of step (2), 18～22 parts of peptones, 14～16 parts of yeast
Extractum, 8～12 parts of chitosans, 2～3 parts of citric acids, 1～2 part of magnesium sulfate and 0.8～1.4 part of potassium dihydrogen phosphate, stir,
And sterilizing, obtain Nutrious fermented thing, Nutrious fermented thing is added to fermentation tank, by inoculum concentration 12～16%, to fermentation tank
The above-mentioned bacterium solution of middle addition, design temperature is 28～35 DEG C, and ventilation is 3～5vvm, standing for fermentation 3～6 days；
(5) after above-mentioned fermentation ends, use tweezers by the antibiotic property bacteria cellulose film on fermentation cylinder for fermentation mixture surface
Take out, be soaked in 1.5mol/L sodium hydroxide solution, after standing 1～2h at 88～94 DEG C, filtered while hot, use distillation
Water washing antibiotic property bacteria cellulose film is to neutral, subsequently by its natural air drying, and sterilizing, antibiotic property antibacterial is fine
Dimension element film.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107982089A (en) * | 2017-12-04 | 2018-05-04 | 上海应用技术大学 | A kind of preparation method of the bacterial cellulose facial mask with bacteriostasis efficacy |
| CN113927965A (en) * | 2021-12-07 | 2022-01-14 | 江南大学 | Photosensitive antibacterial antioxidant composite preservative film based on nano bacterial cellulose and preparation method and application thereof |
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| CN101985641A (en) * | 2010-12-09 | 2011-03-16 | 东华大学 | Method for preparing bacterial cellulose by using wheat straw |
| CN104630311A (en) * | 2015-02-09 | 2015-05-20 | 江苏联海生物科技有限公司 | Method for synchronously producing straw nano-cellulose and bacterial cellulose by using sweet sorghum |
| CN105399988A (en) * | 2015-12-12 | 2016-03-16 | 常州大学 | Preparation method for cellulose/chitosan composite modified degradable antibacterial membrane |
| CN105734093A (en) * | 2016-02-24 | 2016-07-06 | 天津大学 | Preparation and application of ultra-thin bacterial cellulose membrane |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107982089A (en) * | 2017-12-04 | 2018-05-04 | 上海应用技术大学 | A kind of preparation method of the bacterial cellulose facial mask with bacteriostasis efficacy |
| CN113927965A (en) * | 2021-12-07 | 2022-01-14 | 江南大学 | Photosensitive antibacterial antioxidant composite preservative film based on nano bacterial cellulose and preparation method and application thereof |
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